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13 pages, 544 KiB  
Article
Normative Data for Vertical Jump Tests in Pre-School Children Aged 3 to 6 Years
by Vilko Petrić, Sanja Ljubičić and Dario Novak
Biomechanics 2025, 5(3), 56; https://doi.org/10.3390/biomechanics5030056 - 1 Aug 2025
Viewed by 434
Abstract
Background/Objectives: Vertical jump is considered a reliable and valid method of assessing the level of muscular power and coordination across one’s lifespan. The main aim of the present study was to establish sex- and age-normative data for vertical jump outcomes in pre-school [...] Read more.
Background/Objectives: Vertical jump is considered a reliable and valid method of assessing the level of muscular power and coordination across one’s lifespan. The main aim of the present study was to establish sex- and age-normative data for vertical jump outcomes in pre-school children. Methods: We recruited 411 boys and girls aged 3−6 years from four major cities in Croatia and Slovenia. Vertical jump was assessed with two tests: countermovement jump (CMJ) without and with arm swing using a reliable and valid Optojump measuring platform. Data were presented for the 5th, 15th, 25th, 50th (median), 75th, 90th, and 95th percentile. Results: No significant differences were observed in multiple vertical jump outcomes between boys and girls. The mean values for CMJ without and with arm swing between boys and girls were as follows: contact time (1.4 vs. 1.4 s/1.8 vs. 1.7 s), flight time (0.32 vs. 0.31 s/0.33 vs. 0.32), height (12.3 vs. 12.2 cm/13.0 vs. 12.5 cm), power (9.4 vs. 9.5 W/kg/9.3 vs. 9.1 W/kg), pace (0.7 vs. 0.7 steps/s/0.6 vs. 0.6 steps/s), reactive strength index (RSI; 0.10 vs. 0.09 m/s/0.08 vs. 0.08 m/s), and verticality (2.5 vs. 2.3/1.9 vs. 1.9). A gradual increase in all measures according to ‘age’ was observed (p for trend < 0.05). No significant ‘sex*age’ interaction was observed (p > 0.05). Conclusions: This is one of the first studies to provide sex- and age-normative data for complete vertical jump outcomes in pre-school children. These data will serve as an avenue for monitoring and tracking motor development in this sensitive period. Full article
(This article belongs to the Section Sports Biomechanics)
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17 pages, 2003 KiB  
Article
Effect of Caffeinated Chewing Gum on Maximal Strength, Muscular Power, and Muscle Recruitment During Bench Press and Back Squat Exercises
by Li Ding, Jue Liu, Yixuan Ma, Tze-Huan Lei, Mathew Barnes, Li Guo, Bin Chen, Yinhang Cao and Olivier Girard
Nutrients 2025, 17(15), 2455; https://doi.org/10.3390/nu17152455 - 28 Jul 2025
Viewed by 504
Abstract
Background/Objectives: This study aims to investigate the effects of caffeinated chewing gum on maximal strength, muscular power, and neural drive to the prime movers during bench press and back squat in resistance-trained men. Methods: Sixteen resistance-trained males participated in a double-blind, [...] Read more.
Background/Objectives: This study aims to investigate the effects of caffeinated chewing gum on maximal strength, muscular power, and neural drive to the prime movers during bench press and back squat in resistance-trained men. Methods: Sixteen resistance-trained males participated in a double-blind, randomized trial, chewing either caffeinated gum (4 mg/kg) or placebo gum on two separate occasions, seven days apart. After chewing for 5 min, participants performed a maximal strength test followed by muscular power assessments at 25%, 50%, 75%, and 90% of their one-repetition maximum (1RM), completing with 3, 2, 1, and 1 repetition (s), respectively, for bench press and back squat. Surface electromyography data were recorded for each repetition. Results: Caffeinated gum did not significantly improve one-repetition maximum (1RM) for bench press (p > 0.05), but increased mean frequency (MF) and median frequency (MDF) in anterior deltoid, pectoralis major, and biceps brachii (all p < 0.05) compared to placebo. For back squat, 1RM increased with caffeinated gum, along with higher MF and MDF in vastus medialis (all p < 0.05). Caffeinated gum also improved mean and peak velocities, and mean and peak power outputs at 25–75% 1RM during the bench press (all p < 0.05), along with elevated MDF in pectoralis major and biceps brachii (all p < 0.05). Similar improvements were seen in mean and peak velocities during the back squat at 25–90% 1RM (all p < 0.05), along with higher MF and MDF in vastus medialis and increased normalized root mean square activity in gluteus maximus (all p < 0.05). Conclusions: Caffeinated chewing gum (4 mg/kg) enhanced muscular power (25–75% 1RM) in the bench press and improved maximal strength and muscular power (25–90% 1RM) in the back squat by increasing muscle recruitment in resistance-trained men. Full article
(This article belongs to the Special Issue Energy Drink Effectiveness on Human Health and Exercise Performance)
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14 pages, 2603 KiB  
Article
Pulsed Electromagnetic Field (PEMF) Stimulation Increases Muscle Activity During Exercise in Sedentary People
by Aurelio Trofè, Alessandro Piras, Luca Breviglieri, Alessandra Laffi, Andrea Meoni and Milena Raffi
J. Funct. Morphol. Kinesiol. 2025, 10(2), 232; https://doi.org/10.3390/jfmk10020232 - 19 Jun 2025
Viewed by 1114
Abstract
Objectives: A pulsed electromagnetic field (PEMF) induces electric currents in biological tissue, enhancing muscle energy expenditure during heavy constant-load exercises. In this paper, we investigate the PEMF effect on muscular activation in male sedentary people. Methods: The surface electromyographic (EMG) activity of [...] Read more.
Objectives: A pulsed electromagnetic field (PEMF) induces electric currents in biological tissue, enhancing muscle energy expenditure during heavy constant-load exercises. In this paper, we investigate the PEMF effect on muscular activation in male sedentary people. Methods: The surface electromyographic (EMG) activity of the right leg’s vastus medialis (RVM) and biceps femoris (RBF) muscles was recorded and analyzed. The root mean square values were normalized to the peak amplitude observed during maximal voluntary contraction. Measurements were taken at baseline (stationary seated position), during warm-up (unloaded cycling), and throughout 15 min of constant-load exercise performed at moderate intensity. Subjects performed two experimental conditions, when PEMF was turned ON versus OFF. Results: No significant difference was found during the baseline. The analysis during warm-up showed significant differences between conditions (ON vs. OFF) for both muscles (RVM p = 0.019; RBF p < 0.001). The analysis during constant-load exercise showed significant differences between conditions (ON vs. OFF) for RVM only (p = 0.002). Conclusions: This study provides evidence that PEMF stimulation acutely enhances muscle activation, primarily in the vastus medialis, with a comparatively smaller effect on the biceps femoris during moderate-intensity cycling in sedentary young men. The observed increase in EMG activity suggests that PEMF may facilitate neuromuscular excitability and muscle recruitment, potentially through mechanisms related to calcium signaling and enhanced muscle perfusion. Full article
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19 pages, 4233 KiB  
Article
Muscle Recruitment and Asymmetry in Bilateral Shoulder Injury Prevention Exercises: A Cross-Sectional Comparison Between Tennis Players and Non-Tennis Players
by Maite Terré and Mònica Solana-Tramunt
Healthcare 2025, 13(10), 1153; https://doi.org/10.3390/healthcare13101153 - 15 May 2025
Viewed by 889
Abstract
Background/Objectives: Shoulder injuries are common in overhead sports like tennis due to repetitive unilateral movements that can lead to muscle imbalances. This study aimed to compare muscle recruitment and asymmetry during bilateral shoulder injury prevention exercises (performed with both arms simultaneously) in tennis [...] Read more.
Background/Objectives: Shoulder injuries are common in overhead sports like tennis due to repetitive unilateral movements that can lead to muscle imbalances. This study aimed to compare muscle recruitment and asymmetry during bilateral shoulder injury prevention exercises (performed with both arms simultaneously) in tennis players versus non-tennis athletes. Methods: Thirty-nine athletes (sixteen tennis players, twenty-three non-tennis athletes) performed two bilateral scapular retraction exercises at 45° and 90° shoulder abduction. Surface electromyography (sEMG) recorded the activation of the middle and lower trapezius. Root Mean Square (RMS), peak RMS and muscle symmetry indices were analyzed. Results: Tennis players showed significantly lower trapezius activation, especially during prone retraction at 90°. Muscle symmetry was slightly higher in tennis players at 90°, but asymmetry increased at 45°, suggesting angle-specific adaptations. Conclusions: Repetitive asymmetric loading in tennis may reduce the activation of scapular stabilizers and contribute to muscular imbalances. Including targeted bilateral exercises in training may help improve scapular muscle function and reduce injury risk in overhead athletes. Full article
(This article belongs to the Special Issue Common Sports Injuries and Rehabilitation)
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14 pages, 1379 KiB  
Article
Evaluation of Muscle Synergy Flexibility Induced by a Muscle Nerve Block in Chronic Stroke Patients
by Anthony Supiot, Nicolas Roche, Bastien Berret and Didier Pradon
Biomechanics 2025, 5(2), 27; https://doi.org/10.3390/biomechanics5020027 - 1 May 2025
Viewed by 731
Abstract
Background: Drug treatments for gait disorders in post-stroke patients aim to reduce muscular hyperactivity. The analysis of muscle activity is essential to help clinicians understand these disorders. This study aimed to quantify changes in muscle synergies before (PRE) and after (POST) a rectus [...] Read more.
Background: Drug treatments for gait disorders in post-stroke patients aim to reduce muscular hyperactivity. The analysis of muscle activity is essential to help clinicians understand these disorders. This study aimed to quantify changes in muscle synergies before (PRE) and after (POST) a rectus femoris nerve block. Methods: Gait analysis of 8 post-stroke patients before and immediately after nerve block. Muscle synergies were quantified from electromyographic signals. We have selected the account for variance, which indicates the effectiveness of the synergies, the recruitment selectivity index, which indicates the degree of multiplexing of the synergies, and the recruitment consistency index, which indicates the variability of the synergy activations across gait cycles. Results: A decrease in Variance Account For (VAF) is observed, showing a lack of robustness of the underlying muscle synergies between PRE and POST. We also note that spatial and temporal primitives result in different Index of Recruitment Selectivity (IRS) but similar Index of Recruitment Consistency (IRC) for PRE vs. POST. This shows that the synergies’ activations remain consistent across cycles but are more distributed in POST than in PRE. Conclusions: The motor nerve block has not created new muscle synergies of the paretic limb during gait but indicates that there is flexibility in motor organization. This method of quantification may enable clinicians to assess the motor adaptation potential of their post-stroke patients. Full article
(This article belongs to the Section Neuromechanics)
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12 pages, 493 KiB  
Article
Muscle Activation Differences Between CKCUEST and Modified CKCUEST: A Pilot Study
by Samuel Eloy Gutiérrez-Torre, Miguel Ángel Lozano-Melero, Maria Gómez-Jiménez and Daniel Manoso-Hernando
Healthcare 2025, 13(8), 922; https://doi.org/10.3390/healthcare13080922 - 17 Apr 2025
Viewed by 591
Abstract
Background/Objectives: The validity of shoulder orthopaedic tests to establish a diagnosis has recently been challenged. For this reason, functional tests, such as the Closed Kinetic Chain Upper Extremity Stability Test (CKCUEST), have started to be used in clinical settings. The aim of this [...] Read more.
Background/Objectives: The validity of shoulder orthopaedic tests to establish a diagnosis has recently been challenged. For this reason, functional tests, such as the Closed Kinetic Chain Upper Extremity Stability Test (CKCUEST), have started to be used in clinical settings. The aim of this study is to compare the electromyography (EMG) activity during the CKCUEST and the modified CKCUEST in a healthy adult population. Methods: Ten male (age: 26.6 ± 4.8) and ten female participants (age: 24.2 ± 6.0) were recruited from a university setting. The Edinburgh Handedness Inventory, the International Physical Activity Questionnaire and the percentage of activation of the maximum voluntary contraction of the infraspinatus, anterior deltoid and upper trapezius, of both upper limbs, throughout the CKCUEST and modified CKCUEST were analysed. Results: The percentage of activation of the infraspinatus (p < 0.01), anterior deltoid (p < 0.01) and upper trapezius (p < 0.01) in both sides was significantly higher in the CKCUEST compared to the modified CKCUEST. No differences were observed between laterality and the activation percentage of the infraspinatus (p > 0.05), anterior deltoid (p > 0.05) and upper trapezius (p > 0.05) in both sides during the CKCUEST. Conclusions: The results of this research showed a higher percentage of EMG activation during the CKCUEST compared to the modified CKCUEST in all the muscular structures analysed, regardless of the participants’ hemibody. Full article
(This article belongs to the Special Issue Physical Therapy and Rehabilitation in Sports)
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15 pages, 1447 KiB  
Article
Acute Physiological and Perceptual Responses to Six Body-Weight Squat Exercise Variations
by Daniel Santarém, Andreia Teixeira, António Amaral, Jaime Sampaio and Catarina Abrantes
Sensors 2025, 25(7), 2018; https://doi.org/10.3390/s25072018 - 23 Mar 2025
Viewed by 1885
Abstract
Adequate exercise prescription requires a deep understanding of the body’s response to exercise. This study explored the responses of heart rate (HR), muscle oxygen saturation (SmO2), and perceived exertion (RPE) during six body-weight squat exercise variations. A total of 15 recreationally [...] Read more.
Adequate exercise prescription requires a deep understanding of the body’s response to exercise. This study explored the responses of heart rate (HR), muscle oxygen saturation (SmO2), and perceived exertion (RPE) during six body-weight squat exercise variations. A total of 15 recreationally active participants (age: 28.2 ± 8.0 years; body mass: 71.1 ± 11.2 kg; height: 1.73 ± 0.08 m) were recruited. Six body-weight squat variations (deep, jumping, single-leg, uneven, unstable, and wall-sit) were randomly performed for 90 s. Results revealed that the jumping squat promoted a higher average and peak HR (165.3 ± 14.5 and 146.1 ± 14.8 bpm, respectively), and a lower average SmO2 and higher deoxygenation SmO2 in the soleus muscle (40.3 ± 15.4 and 46.0 ± 11.4%, accordingly). No differences were observed in recovery time or in the same SmO2 derived-parameters in the vastus lateralis muscle. The jumping variation promoted a greater response at a physiological level, both centrally, related to cardiovascular response, and peripherally, related to soleus SmO2. It was also the more demanding variation at both the overall and lower limb muscular level of RPE. This holistic view allows a precise identification of the response patterns in body-weight squat exercise variations to an acute session, with a training intervention providing additional information. Full article
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15 pages, 3962 KiB  
Article
Biomechanical Insights into Ski Mountaineering: Kinematics and Muscular Activation in Uphill Movements
by Lucia Donno, Carlalberto Francia, Filippo Motta, Antonella LoMauro, Carlo Gorla, Diego Scaccabarozzi, Marco Tarabini and Manuela Galli
Appl. Sci. 2025, 15(3), 1003; https://doi.org/10.3390/app15031003 - 21 Jan 2025
Viewed by 1521
Abstract
Ski mountaineering (Skimo) combines mountain climbing and skiing, with ascents on skis or by carrying them, followed by ski descents. Despite its popularity, the literature lacks integrated biomechanical analyses during indoor training, limiting evidence-based recommendations for training, injury prevention, and performance improvement in [...] Read more.
Ski mountaineering (Skimo) combines mountain climbing and skiing, with ascents on skis or by carrying them, followed by ski descents. Despite its popularity, the literature lacks integrated biomechanical analyses during indoor training, limiting evidence-based recommendations for training, injury prevention, and performance improvement in this sport. This study analyzed four Skimo athletes during uphill walking and running on a 9° inclined treadmill at 7 km/h and 8 km/h. Kinematics was assessed by an optoelectronic system, and surface electromyography recorded the muscular activity of twelve bilateral muscles (Erector Spinae, Rectus Abdominis, Rectus Femoris, Biceps Femoris, Tibialis Anterior, Gastrocnemius Lateralis). Rectus Femoris and Biceps Femoris co-activation supported knee stability during the load support phase, especially while running. Running at 8 km/h was the most demanding condition, with increased knee flexion throughout the task cycle. Switching from 7 to 8 km/h reduced the maximum extension and increased the maximum flexion of both hip and knee and required the Gastrocnemius’ recruitment in the swing phase. Regardless of task and speed, Rectus Abdominis and Erector Spinae played a key role in stabilizing the trunk. This study provided a biomechanical characterization of two motor gestures typical in Skimo, highlighting how task typology and velocity could influence kinematics and muscle activation. Full article
(This article belongs to the Special Issue Human Biomechanics and EMG Signal Processing)
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17 pages, 598 KiB  
Article
Muscular Performance and Blood Pressure After Different Pre-Strength Training Strategies in Recreationally Strength-Trained Women: Cross-Over Trial
by Estêvão Rios Monteiro, Linda S. Pescatello, Luis Leitão, Marcelo José Colonna de Miranda, Paulo H. Marchetti, Michelle Ribeiro Novaes, Gleisson da Silva Araújo, Victor Gonçalves Corrêa Neto and Jefferson da Silva Novaes
J. Cardiovasc. Dev. Dis. 2025, 12(1), 7; https://doi.org/10.3390/jcdd12010007 - 26 Dec 2024
Cited by 1 | Viewed by 1336
Abstract
Background: This study aimed to investigate the acute effects of different pre-ST strategies on muscular performance and blood pressure (BP) responses in recreationally strength-trained women. Methods: Twelve overweight women with normal BP were recruited and performed six experimental protocols in a randomized order: [...] Read more.
Background: This study aimed to investigate the acute effects of different pre-ST strategies on muscular performance and blood pressure (BP) responses in recreationally strength-trained women. Methods: Twelve overweight women with normal BP were recruited and performed six experimental protocols in a randomized order: (1) control protocol (CC), where BP was assessed without exercises performed; (2) ST; (3) foam rolling warm-up followed by ST (FR + ST); (4) specific warm-up followed by ST (SW + ST); (5) aerobic exercise followed by ST (AE + ST); and (6) stretching exercises followed by ST (SE + ST). ST consisted of three sets at 80% of 10 repetition maximum with a self-suggested rest interval between sets for bench press, back squat, bench press 45°, front squat, lat pull-down, leg press, shoulder press, and leg extension. Results: All experimental protocol had a lower total training volume, fatigue index, and repetitions performance in relation to ST (p < 0.05). No significant reduction was observed in systolic and diastolic BP for any protocol or exercise, although the effect size magnitudes ranged from trivial to large. Decreases in maximum repetitions, resistance to fatigue, and total training volume were performed before ST as warm-up strategies. However, these strategies indicated a clinical reduction in BP with a large and meaningful magnitude (effect size) in recreationally strength-trained women with normal to elevated BP. Conclusions: The results of this investigation may help to influence decision-making by practitioners who desire to elicit a post-exercise hypotension response in both subjects with normal BP and hypertension. Full article
(This article belongs to the Special Issue Cardiovascular Disease: Risk Factors and Prevention)
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11 pages, 559 KiB  
Article
Electromyographic Diagnostic Ranges Defining Temporomandibular Disorders and Healthy Individuals’ Results in Functional Clenching Index
by Grzegorz Zieliński and Michał Ginszt
J. Clin. Med. 2025, 14(1), 14; https://doi.org/10.3390/jcm14010014 - 24 Dec 2024
Cited by 2 | Viewed by 822
Abstract
Background: Temporomandibular disorders (TMDs) represent a significant public health issue, among which masticatory muscle pain is the most common. Current publications increasingly indicate surface electromyography (sEMG) as an effective diagnostic tool for muscle dysfunctions that may be employed in TMDs recognition. The [...] Read more.
Background: Temporomandibular disorders (TMDs) represent a significant public health issue, among which masticatory muscle pain is the most common. Current publications increasingly indicate surface electromyography (sEMG) as an effective diagnostic tool for muscle dysfunctions that may be employed in TMDs recognition. The objective of this study was to establish reference ranges for TMDs patients with masticatory muscle pain and healthy individuals in the electromyographic Functional Clenching Index (FCI) for the temporalis muscles (TAs) and masseter muscles (MMs). This research aimed to provide an additional diagnostic tool for TMDs patients. Methods: A total of 48 individuals (n = 30 women and n = 18 men) with the muscular painful form of TMDs were recruited alongside a numerically and gender-matched control group—healthy, pain-free controls. The Functional Clenching Indexwas calculated for both groups. Results: Data analysis revealed statistically significant differences with a very large effect size. Healthy individuals had higher FCI scores compared to those with TMDs. The healthy group exhibited higher threshold values compared to the TMDs group. Conclusions: For healthy individuals, the FCI ranges for TAs were between 58 and 145, while for MMs, between 72 and 210. Lower values may indicate muscle activation disorders and occur in patients with the painful, muscular form of TMDs. This is the first study to define reference ranges for electromyographic indices; therefore, caution is recommended, and the replication of this study on a larger and more culturally diverse sample is advised. Full article
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12 pages, 1695 KiB  
Technical Note
Personality Fuels the Fire: Predicting Firefighter Physical Readiness
by Annmarie Chizewski and Steven J. Petruzzello
Fire 2024, 7(12), 465; https://doi.org/10.3390/fire7120465 - 6 Dec 2024
Cited by 1 | Viewed by 1208
Abstract
Many firefighters have inadequate levels of physical fitness which can impair firefighting performance. Understanding the factors that influence exercise and fitness behaviors could help identify those less likely to stay physically fit. Methods: A sample (N = 45) of male recruit firefighters [...] Read more.
Many firefighters have inadequate levels of physical fitness which can impair firefighting performance. Understanding the factors that influence exercise and fitness behaviors could help identify those less likely to stay physically fit. Methods: A sample (N = 45) of male recruit firefighters were assessed during weeks 1 and 7 of a state firefighter academy. Measures included cardiovascular fitness, muscular fitness, performance on firefighter ability tasks, exercise intensity preference/tolerance, and extraversion. Results: Exercise intensity preference was directly related to physical fitness, which in turn was directly related to firefighter ability. Regression analyses showed that exercise intensity preference predicted variance in firefighter ability, and this relationship was statistically mediated by physical fitness levels. Firefighters with higher exercise intensity preference tended to have better physical fitness, which was associated with superior performance on firefighter tasks. Conclusions: These findings suggest that firefighters’ exercise intensity preferences play a key role in their physical fitness and, ultimately, their firefighting abilities. Assessing recruits’ exercise intensity preferences could help identify those who may struggle to maintain fitness, allowing for targeted interventions. Improving firefighters’ exercise intensity preference may be an effective strategy for enhancing their physical capabilities and job performance. Full article
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19 pages, 357 KiB  
Review
Low-Intensity Resistance Exercise in Cardiac Rehabilitation: A Narrative Review of Mechanistic Evidence and Clinical Implications
by Jemima Jansen, Paul W. Marshall, Jocelyne R. Benatar, Rebecca Cross, Tia K. Lindbom and Michael Kingsley
J. Clin. Med. 2024, 13(23), 7338; https://doi.org/10.3390/jcm13237338 - 2 Dec 2024
Cited by 4 | Viewed by 3210
Abstract
Cardiac rehabilitation, a multi-component intervention designed to mitigate the impact of cardiovascular disease, often underutilises low-intensity resistance exercise despite its potential benefits. This narrative review critically examines the mechanistic and clinical evidence supporting the incorporation of low-intensity resistance exercise into cardiac rehabilitation programmes. [...] Read more.
Cardiac rehabilitation, a multi-component intervention designed to mitigate the impact of cardiovascular disease, often underutilises low-intensity resistance exercise despite its potential benefits. This narrative review critically examines the mechanistic and clinical evidence supporting the incorporation of low-intensity resistance exercise into cardiac rehabilitation programmes. Research indicates that low-intensity resistance exercise induces hypertrophic adaptations by maximising muscle fibre activation through the size principle, effectively recruiting larger motor units as it approaches maximal effort. This activation promotes adaptation in both type I and II muscle fibres, resulting in comparable increases in myofibrillar protein synthesis and phosphorylation of key signalling proteins when compared to high-intensity resistance exercise. Low-intensity resistance exercise provides equivalent improvements in muscular strength and hypertrophy compared to high-intensity protocols while addressing barriers to participation, such as concerns about safety and logistical challenges. By facilitating engagement through a more accessible exercise modality, low-intensity resistance exercise might improve adherence rates and patient outcomes in cardiac rehabilitation. Additionally, the ability of low-intensity resistance exercise to address sarcopenia and frailty syndrome, significant determinants of cardiovascular disease progression, can enhance the recovery and overall quality of life for patients. This review establishes evidence-based recommendations for the inclusion of low-intensity resistance exercise in cardiac rehabilitation, offering a promising pathway to enhance the effectiveness of these programmes. Full article
(This article belongs to the Section Clinical Rehabilitation)
14 pages, 1315 KiB  
Article
Organ Tropism of Angiostrongylus vasorum Larval Stages in Infected African Giant Snails (Lissachatina fulica)
by Alena Dusch, Lisa Segeritz, Manfred Henrich, Anja Taubert and Carlos Hermosilla
Pathogens 2024, 13(11), 946; https://doi.org/10.3390/pathogens13110946 - 30 Oct 2024
Viewed by 1412
Abstract
Angiostrongylus vasorum is a metastrongyloid lungworm causing severe cardiovascular disease in domestic and wild animals. During its heteroxenous life cycle, A. vasorum requires obligate gastropod intermediate hosts. Little is known about A. vasorum larval organ tropism and development in gastropod intermediate hosts. Thus, [...] Read more.
Angiostrongylus vasorum is a metastrongyloid lungworm causing severe cardiovascular disease in domestic and wild animals. During its heteroxenous life cycle, A. vasorum requires obligate gastropod intermediate hosts. Little is known about A. vasorum larval organ tropism and development in gastropod intermediate hosts. Thus, the aim of this study was to analyze in vivo development of A. vasorum larval stages in experimentally infected African giant snails (Lissachatina fulica). Adult L. fulica (n = 26) were orally infected with A. vasorum-L1 and thereafter continuously euthanized. Gastropod organs were artificially digested and microscopically analyzed for the presence of A. vasorum larvae. Moreover, paraffin-fixed organs were investigated histologically for snail-borne innate immune response. In the current study, the success of L. fulica oral infection was demonstrated, thereby reaching larval recovery rates of up to 49.7%. During snail infection, an organ tropism of A. vasorum larvae was detected for the lungs and the foot muscular tissue. Overall, A. vasorum-driven gastropod innate immune reactions against larvae varied greatly. In some specimens, larvae were found effectively ensnared by recruited hemocytes, resulting in granuloma formation, whilst in others, hemocyte-mediated reactions were barely observed. Nevertheless, these evidences demand more studies on hemocyte-derived effector mechanisms against A. vasorum. Full article
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11 pages, 1809 KiB  
Article
Validation of Fixed Ultrasonography for Achilles Tendon Assessment: A Reliability Study
by Raquel Alabau-Dasi, Gabriel Dominguez-Maldonado, Ana Belen Ortega-Avila, Luis M. Gordillo-Fernandez, Mercedes Ortiz-Romero, Juan Manuel Melchor-Rodriguez and Gabriel Gijon-Nogueron
Diagnostics 2024, 14(19), 2221; https://doi.org/10.3390/diagnostics14192221 - 5 Oct 2024
Viewed by 1498
Abstract
Background: It is important to highlight the advantages of ultrasound in assessing muscular and tendinous behavior due to its non-invasive nature and capacity for dynamic studies. However, evaluating tendons via ultrasound can be challenging given the complexity of anisotropic phenomena related to collagen [...] Read more.
Background: It is important to highlight the advantages of ultrasound in assessing muscular and tendinous behavior due to its non-invasive nature and capacity for dynamic studies. However, evaluating tendons via ultrasound can be challenging given the complexity of anisotropic phenomena related to collagen fiber arrangement. This study aims to validate the reliability of fixed ultrasound compared to manual acquisition in measuring Achilles tendon thickness. Method: Twenty participants, six men and fourteen women, were recruited. Ultrasound was used to measure the Achilles tendon’s thickness at two specific points (4 and 6 cm from the calcaneal insertion of the Achilles tendon). The measurements were conducted by two examiners, one with previous experience and another without. Results: The measurements at 6 cm from the calcaneal insertion showed α = 0.996, α = 0.998 for measurements at 4 cm using manual acquisition, and α = 0.997 for measurements with fixed ultrasound at rest. For the weight-bearing and ankle dorsiflexion measurements, the reliability was excellent (α = 0.999 and α = 1.000). Conclusions: The findings demonstrated excellent reliability in the ultrasound measurements of the Achilles tendon’s thickness, even when performed by different evaluators and under load-bearing conditions. This study suggests the clinical utility of assessing anatomical structures under load, enhancing ultrasound’s applicability beyond the examination table. It is concluded that fixed ultrasound acquisition exhibits excellent reliability in measuring the Achilles tendon’s thickness, offering potential benefits for precise diagnosis of pathologies, planning surgical interventions, and reducing possible errors related to operator variability. Full article
(This article belongs to the Special Issue Clinical Diagnosis and Treatment of Chronic Pain, Second Edition)
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12 pages, 573 KiB  
Article
Association of Physical Performance with Mental and Physical Health-Related Quality of Life and Low Back Pain-Related Disabilities among Older Adults with Severe Obesity
by Munkh-Erdene Bayartai, Gabriella Tringali, Roberta De Micheli, Ana Lúcia Danielewicz and Alessandro Sartorio
J. Clin. Med. 2024, 13(18), 5614; https://doi.org/10.3390/jcm13185614 - 22 Sep 2024
Viewed by 2224
Abstract
Background: Low back pain is one of the most prevalent musculoskeletal problems and continues to be the leading cause of disabilities worldwide. The aim of this study was to cross-sectionally investigate the association of physical performance with mental and physical health-related quality of [...] Read more.
Background: Low back pain is one of the most prevalent musculoskeletal problems and continues to be the leading cause of disabilities worldwide. The aim of this study was to cross-sectionally investigate the association of physical performance with mental and physical health-related quality of life and low back pain-related disabilities among older adults with severe obesity. Methods: A total of 96 hospitalized older adults with severe obesity (45 males, 51 females, age: 69.7 ± 5.4 years; BMI: 43.7 ± 5.7 kg/m2) were recruited into the study. Physical performance, health-related quality of life, and low back pain-related disability were measured through physical performance tests, the 12-item short-form survey (SF-12), and the Oswestry disability index, respectively. Results: LBP-related disabilities, as well as physical health-related quality of life, were associated with all the physiological parameters measured by physical performance tests, including muscular strength, aerobic capacity, balance, and lower body flexibility (p < 0.05). In contrast, mental health-related quality of life was associated with fewer physiological parameters, such as primarily muscular strength (p < 0.05). Conclusions: These findings could provide important insights for developing rehabilitation strategies designed to improve LBP-related disabilities, as well as the physical and mental health-related quality of life, in older adults with severe obesity. Full article
(This article belongs to the Section Endocrinology & Metabolism)
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