Search Results (68)

The objective of this study was to investigate the temporal effectiveness of repetitive peripheral magnetic stimulation (rPMS) on lower-limb motor skill performance in individuals with chronic stroke. In this sham-controlled crossover study, we hypothesized that individuals with stroke who received rPMS would demonstrate improved motor skill performance after the stimulation and maintain this enhanced performance at 30 and 60 min after the stimulation. Sixteen participants performed a visuomotor ankle-tracking task at multiple time points following either rPMS or sham stimulation. rPMS, delivered to the tibialis anterior muscle, did not result in statistically significant changes in spatiotemporal (p = 0.725) or spatial error (p = 0.566) metrics at any post-stimulation time point. These findings suggest that a single session of rPMS does not lead to measurable improvements in lower-limb motor skill performance in individuals with stroke, underscoring the need to refine stimulation parameters and target populations in future protocols. Full article

Background: Lower limb motor output contributes to determining functional performance in many motor tasks. This study investigated the effects of non-invasive spinal cord transcutaneous stimulation (scTS) applied during an exercise-based priming protocol on lower limb muscle force and power generation. Methods: Twelve young, physically active male volunteers (age: 22.7 ± 2.1 years) participated in this randomized crossover, sham-controlled study. The maximal voluntary contraction and low-level torque steadiness of knee extensors, as well as the maximal explosive extension of lower limbs, were assessed before and after the priming protocol with scTS or sham stimulation over a total of four experimental sessions. Further, characteristics of evoked potentials to scTS related to spinal circuitry excitability were assessed in the supine position before and after the scTS priming protocol. The exercise component of the ~25 min priming protocol consisted of low-volume, low- and high-intensity lower limb motor tasks. Results: scTS priming protocol tended to increase or maintain maximum isometric torque during knee extension (4.7%) as well as peak force (0.2%) and rate of force development (6.0%) during explosive lower limb extensions, whereas sham priming protocol tended to decrease them (−4.3%, −3.3%, and −15.1%, respectively). This resulted in significant interactions (p = 0.001 to 0.018) and medium–large differences between scTS and sham protocols. These findings were associated with meaningful trends of some neurophysiological variables. Conversely, priming protocols did not affect low-level torque steadiness. Conclusions: scTS counteracted the unexpected fatigue induced by the exercise-based priming protocol, supporting lower limb performance during maximal efforts. Future studies are warranted to assess the implementation of scTS with optimized exercise-based priming protocols during training and rehabilitation programmes that include high-intensity neuromuscular efforts. Full article

Parkinson’s disease (PD) is a neurodegenerative disorder primarily defined by the deterioration of motor function and characterized by the loss of dopaminergic neurons in the nigrostriatal system. Although it is the second most prevalent disorder of the central nervous system, current treatments primarily focus on symptom management and modestly slowing disease progression, ultimately failing to preserve the long-term quality of life of a substantial proportion of affected individuals. Innovative therapies that can restore neuronal function have emerged, such as the use of the secretome of Mesenchymal Stem Cells (MSCs) due to their rich composition of bioactive molecules. This therapy exhibits robust paracrine activity that drives most of the self-renewal capacity, differentiation potential, and immune regulation of MSCs without presenting compatibility issues often associated with stem cell-based therapies. While conceptually appealing, the clinical application of this approach is still limited by the availability and proliferation capacity of MSCs, as it impacts not only secretome production but also its quality. Various protocols have been developed to enhance secretome action by adding various compounds to cell culture media, given the high environmental plasticity of MSCs. Some of the compounds already used are Caloric Restriction Mimetics (CRMs), molecules that mimic Caloric Restriction (CR) conditions, which have been demonstrated to extend lifespan and reduce age-related diseases in various organisms. While not sufficient to cure neurodegenerative disorders, these compounds may potentiate secretome efficiency by enhancing autophagy pathways and relieving oxidative stress burden from MSCs. Therefore, in this article, we aim to explore the effects of CRMs priming on MSCs and how it may help bridge existing gaps in regenerative therapies for PD. Full article

Introduction: Basketball performance requires not only intermittent high-intensity movements—such as sprinting, jumping, and rapid directional changes—but also rapid decision-making under cognitive and psychological stress. Transcranial direct current stimulation (tDCS) has emerged as a potential modality to enhance both physical and mental performance due to its capacity to modulate cortical excitability and promote synaptic plasticity. Although the broader literature suggests that tDCS can benefit motor performance and endurance across various sports, its specific impact on basketball remains underexplored. Methods: This scoping review aimed to summarize current evidence on the effects of tDCS in basketball. A comprehensive literature search was conducted across databases including PubMed/Medline, Google Scholar, and Cochrane, identifying studies published between January 2008 and February 2025. Only clinical trials investigating tDCS interventions in basketball players were included. Eleven articles met the inclusion criteria and were synthesized narratively, with a focus on stimulation parameters (site, duration, intensity) and performance outcomes (shooting accuracy, dribbling, sprinting, decision-making, fatigue). Results: The reviewed studies indicated that tDCS—particularly when applied over the motor cortex—was associated with moderate improvements in shooting accuracy, dribbling time, repeated-sprint performance, and decision-making under fatigue. Some studies reported delayed rather than immediate benefits, suggesting that tDCS may prime neural networks for enhanced learning and retention. However, not all findings were consistent; certain interventions produced minimal or no significant effects, especially regarding subjective mental fatigue and cognitive workload. The variability in electrode placements and stimulation protocols highlights the need for methodological standardization. Conclusions: Current evidence partially supports the potential of tDCS to improve specific performance domains in basketball, particularly in skill acquisition, neuromuscular efficiency, and decision-making. Nevertheless, the findings are limited by small sample sizes, heterogeneous protocols, and a lack of long-term follow-up. Future research should prioritize larger, multisite studies with standardized tDCS parameters and ecologically valid outcome measures to confirm the efficacy and practical relevance of tDCS in competitive basketball settings. Full article

Background/Objectives: When manipulating an object placed on the palm, the eyes and fingers move together. To perform this task precisely, coordination of the eyes and fingers is needed. Based on this view, the present study examined the three-way interaction among the central motor drives to the eyes, index finger, and little finger. Methods: Healthy male participants abducted the right index and/or little finger with or without concomitant saccadic eye movement to the right in response to a visual cue, while the forearm was in the pronated or supinated position. We measured the reaction time (RT), velocity, and amplitude of the eye movements, as well as the RT and amplitude of the electromyographic (EMG) responses in the prime movers for the independent and dependent finger movements. Results: The velocity, amplitude, and RT of the eye movement were not changed by the additional involvement of the finger movement, indicating that the central motor drive to the finger does not influence the eye motor excitability and central motor drive to the eyes. On the one hand, the RT of the finger was not changed by the eye movement, indicating that the central motor drive to the eyes does not influence the central motor drive to the finger muscle. On the other hand, the EMG amplitude in the first dorsal interosseous muscle at the movement onset decreased during the concomitant eye movement, indicating that the central motor drive to the eyes suppresses the motor excitability of the independent finger muscle. The RT increased and EMG amplitude decreased in one finger muscle when the other finger concurrently moved, indicating that the central motor drive to one finger muscle suppresses the motor excitability of and central motor drive to the other finger muscle. The change in the RT and EMG amplitude in one finger muscle caused by the concomitant execution of the other finger movement and/or eye movement varied with forearm position, indicating that forearm proprioception influences the interaction of the motor execution processes among the fingers and eyes. Conclusions: The central motor drive to the eyes or finger muscles suppresses the motor excitability of the other finger muscles and the central motor drive to that muscle, but the central motor drive to the finger muscles does not influence those for the eyes. Forearm proprioception influences the motor excitability of the finger muscle and central motor drive to that muscle. Full article

Background: Children with unilateral cerebral palsy (UCP) frequently experience limitations in locomotor abilities, attributable to a complex interplay of factors including muscle weakness and reduced joint mobility. Movement-based priming, such as cross-training (CT), has emerged as a potential intervention to enhance motor function in children with UCP. However, evidence of its efficacy remains limited. Objective: This study aimed to investigate the effect of CT—specifically, a strengthening-based unilateral priming protocol—on muscle strength, joint mobility, and locomotor abilities in children with UCP. Methods: Thirty-six children with UCP were enrolled in a randomized controlled trial. Participants were randomized into two groups: the control group (n = 18; underwent a tailored physical rehabilitation program) and the CT group (n = 18; participated in a CT program incorporating unilateral priming exercises targeting the less-affected lower followed by the same rehabilitation program administered to the control group). Dorsiflexor strength, ankle joint mobility, and locomotor ability assessed via the 10 m walk test (10 mWT), 6 min walk test (6 MWT), and timed up-and-go test (TUG) were evaluated pre- and post-intervention. Results: Significant moderate-to-large between-group differences were observed in dorsiflexor strength (p = 0.032, partial η² = 0.128), ankle mobility (p = 0.016, partial η² = 0.159), and locomotor ability (10 mWT [p = 0.017, partial η² = 0.157]; 6 MWT [p = 0.004, partial η² = 0.222]; TUG [p = 0.047, partial η² = 0.111]). The CT group demonstrated superior improvements concerning all outcomes. Conclusions: Unilateral priming through strengthening-based CT is a viable intervention for enhancing motor function in children with UCP, providing a promising complement to the current rehabilitation protocols. Full article

Background: Amyotrophic lateral sclerosis (ALS) is a multifactorial neurodegenerative disease characterized by the involvement of multiple pathways and mechanisms. The complexity of its pathophysiology is reflected in the diverse hypotheses relating to its underlying causes. Given this intricate interplay of processes, a combination therapy approach offers a promising strategy. Combination therapies have demonstrated significant success in treating complex diseases, where they aim to achieve synergistic therapeutic effects and reduce drug dosage. PrimeC is an oral combination treatment composed of a patented novel formulation consisting of specific and unique doses of two well-characterized drugs (ciprofloxacin and celecoxib). It aims to synergistically inhibit the progression of ALS by addressing key elements of its pathophysiology. Objectives: Demonstrating the synergistic effect of the PrimeC combination compared to each of its individual components, celecoxib and ciprofloxacin, and assessing its ability to improve the drug concentration profile and efficacy. Methods: The efficacy of the PrimeC combination was assessed in a survival assay using human induced pluripotent stem cell (iPSC)-derived motor neurons. Additionally, a drug profiling study was conducted, measuring drug levels in the brain and serum of C57BL mice treated with a single compound versus the combination. Results: Motor neurons modeling ALS treated with the PrimeC combination exhibited better survival rates compared to treatment with either individual compound alone. The enhanced efficacy of the combination was further supported by a drug concentration profiling study in rodents, demonstrating that the PrimeC combination resulted in increased ciprofloxacin concentrations in both brain tissue and serum—highlighting the optimized interaction and synergistic potential of its two comprising agents. Conclusions: Our findings support the potential of combination therapy as an effective strategy for ALS treatment. Specifically, the PrimeC combination demonstrated promising therapeutic effects, providing a strong rationale for its ongoing development as a targeted treatment for ALS. Full article

Background/Objectives: TDP-43 mutation-driven Amyotrophic Lateral Sclerosis (ALS) motor neuron disease is one of the most prominent forms (approximately 97%) in cases of sporadic ALS. Dysfunctional autophagy and lysosomal function are the prime mechanisms behind ALS. Mitoxantrone (Mito), a synthetic doxorubicin analog, is an inhibitor of DNA and RNA synthesis/repair via intercalating with nitrogenous bases and inhibiting topoisomerase II. The therapeutic potential of miRNAs associated with disease conditions has also been reported. This study explores the therapeutic potential of Mito along with miRNAs against mutated TDP-43 protein-induced proteinopathy in human-induced pluripotent stem cell (hiPSC)-derived human neural progenitor cells (hNPCs). Methods: HiPSCs mutated for TDP-43 were differentiated into hNPCs and used to explore the therapeutic potential of Mito at a concentration of 1 μM for 24 h (the identified non-cytotoxic dose). The therapeutic effects of Mito on miRNA expression and various cellular parameters such as mitochondrial dynamics, autophagy, and stress granules were assessed using the high-throughput Open Array technique, immunocytochemistry, flow cytometry, immunoblotting, and mitochondrial bioenergetic assay. Results: Mutated TDP-43 protein accumulation causes stress granule formation (G3BP1), mitochondrial bioenergetic dysfunction, SOD1 accumulation, hyperactivated autophagy, and ER stress in hNPCs. The mutated hNPCs also show dysregulation in six miRNAs (miR-543, miR-34a, miR-200c, miR-22, miR-29b, and miR-29c) in mutated hNPCs. A significant restoration of TDP-43 mutation-induced alterations could be witnessed upon the exposure of mutated hNPCs to Mito. Conclusions: Our study indicates that miR-543, miR-29b, miR-22, miR-200c, and miR-34a have antisense therapeutic potential alone and in combination with Mitoxantrone. Full article

Background: Occupational therapy (OT) interventions on activities of daily living (ADL), cognitive functions, and physical function in middle-aged and older people with chronic stroke. Methods: A systematic review search until November 2024 using five generic databases: PubMed/Medline, Web of Science, Scopus, ScienceDirect, and OT seeker. The PRISMA checklist, RoB 2 (Cochrane, London, UK), and GRADEpro (Evidence Prime Inc., Hamilton, CA) tools assessed the evidence’s methodological quality and certainty. The protocol was registered in PROSPERO (code: CRD42024568225). Results: Of 1733 records were identified across the databases, nine studies were analyzed using the PICOS format. The meta-analysis revealed significant improvements in independent performance of activities of daily living (ADL), as measured by the Canadian Occupational Performance Measure (COPM), in favor of the experimental groups (p = 0.03). No significant differences were found for the other variables analyzed. Conclusions: Performance on ADLs improved significantly according to the COPM, whereas no significant improvements in cognitive or physical function were observed among middle-aged and older chronic stroke survivors. Individual studies highlight the potential benefits of OT interventions that combine cognitive, motor, and technological approaches, such as virtual reality and brain stimulation. Full article

This paper presents the design and performance evaluation of an experimental platform that emulates the static and dynamic behavior of a 3 kW Wind Energy Conversion System (WECS). The platform includes a wind turbine emulator (WTE) using a separately excited DC motor (SEDCM) as the prime mover, coupled with a grid-connected doubly-fed induction generator (DFIG). This setup enables comprehensive laboratory studies of a WECS without the need for large-scale field installations. A novel inertia compensation strategy is implemented to ensure the SEDCM accurately replicates the power and torque characteristics of a real wind turbine across various wind profiles. The DFIG was chosen for its high efficiency at variable wind speeds and its reduced power converter requirements compared to other generators. The control strategy for the DFIG is detailed, highlighting its performance and seamless integration within the system. Unlike most studies focusing on generators connected to simple loads, this research considers a grid-connected system, which introduces additional challenges and requirements. This study thoroughly investigates the grid-connected converter, addressing specific demands for grid connection and ensuring compliance with grid standards. Experimental results validate the effectiveness of the emulator, demonstrating its potential as a key tool for optimizing wind turbine control strategies and real-time algorithm validation, and enhancing the performance and reliability of renewable energy systems. Full article

The binding of small molecules to sarcomeric myosin can elicit powerful effects on the chemomechanical cycle, making them effective therapeutics in the clinic and research tools at the benchtop. However, these myotropes can have complex effects that act on different phases of the crossbridge cycle and which depend on structural, dynamic, and environmental variables. While small molecule binding sites have been identified crystallographically and their effects on contraction studied extensively, small molecule-induced dynamic changes that link structure–function are less studied. Here, we use molecular dynamics simulations to explore how omecamtiv mecarbil (OM), a cardiac myosin-specific myotrope, alters the coordinated dynamics of the lever arm and the motor domain in the pre-powerstroke state. We show that the lever arm adopts a range of orientations and find that different lever arm orientations are accompanied by changes in the hydrogen bonding patterns near the converter. We find that the binding of OM to myosin reduces the conformational heterogeneity of the lever arm orientation and also adjusts the average lever arm orientation. Finally, we map out the distinct conformations and ligand–protein interactions adopted by OM. These results uncover some structural factors that govern the motor domain–tail orientations and the mechanisms by which OM primes the pre-powerstroke myosin heads. Full article

This paper investigated a new speed regulator using an adaptive transitioning policy iteration learning technique for the variable reluctance motor (VRM) drive. A transitioning strategy is used in this unique scheme to handle the nonlinear behavior of the VRM by using a series of learning centers, each of which is an individual local learning controller at linear operational location that grows throughout the system’s nonlinear domain. This improved control technique based on an adaptive dynamic programming algorithm is developed to derive the prime solution of the infinite horizon linear quadratic tracker (LQT) issue for an unidentified dynamical configuration with a VRM drive. By formulating a policy iteration algorithm for VRM applications, the speed of the motor shows inside the machine model, and therefore the local centers are directly affected by the speed. Hence, when the speed of the rotor changes, the parameters of the local centers grid would be updated and tuned. Additionally, a multivariate transition algorithm has been adopted to provide a seamless transition between the Q-centers. Finally, simulation and experimental results are presented to confirm the suggested control scheme’s efficacy. Full article

Aim: The aim was to evaluate the effect of body actions on learning process, particularly semantic memory capabilities in drug-naïve children with attention deficit hyperactivity disorder (ADHD). Method: Thirty children had to listen to a story which was repeated three times in a row and then a fourth time five minutes later. After each listen, the child was asked what she/he remembered from the story. The whole sample was split randomly into three subgroups of equal IQ (mean 102.2 ± 12.7), age (mean age 8 ± 0.6 years), sex (ratio female to male 1:5) and severity of ADHD symptoms (34.2 ± 7.4); a G1 “Freeze” subgroup, which implied listening to the story while sitting on a chair without moving; a G2 “Minimal” subgroup, which implied listening to the story while sitting on a chair but free movement was allowed; a G3 “Prescribed movement” subgroup, which implied listening to the story standing up, while copying the experimenter movements that mimicked the actions told in the story. Results: Although our sample was limited in size, interestingly, children in the G3 subgroup showed the highest short-term semantic memory retention compared to G1. In all subgroups, repetition allowed an increase in performance. Conclusions: Our exploratory findings stress the positive role of movement in children with ADHD to increase semantic memorization. Hyperactivity may counteract the deficit of memorization related to attention impairment in children with ADHD. Our results may encourage parents or teachers to allow children with ADHD to move around during short-term memory-retention tasks. Full article

Thyrotropin-releasing hormone (TRH; pGlu-His-Pro-NH2) is an intercellular signal produced mainly by neurons. Among the multiple pharmacological effects of TRH, that on food intake is not well understood. We review studies demonstrating that peripheral injection of TRH generally produces a transient anorexic effect, discuss the pathways that might initiate this effect, and explain its short half-life. In addition, central administration of TRH can produce anorexic or orexigenic effects, depending on the site of injection, that are likely due to interaction with TRH receptor 1. Anorexic effects are most notable when TRH is injected into the hypothalamus and the nucleus accumbens, while the orexigenic effect has only been detected by injection into the brain stem. Functional evidence points to TRH neurons that are prime candidate vectors for TRH action on food intake. These include the caudal raphe nuclei projecting to the dorsal motor nucleus of the vagus, and possibly TRH neurons from the tuberal lateral hypothalamus projecting to the tuberomammillary nuclei. For other TRH neurons, the anatomical or physiological context and impact of TRH in each synaptic domain are still poorly understood. The manipulation of TRH expression in well-defined neuron types will facilitate the discovery of its role in food intake control in each anatomical scene. Full article

Background: Gambling Disorder (GD) is a bio-psycho-social disorder resulting from the interaction of clinical, cognitive, and affective factors. Impulsivity is a crucial factor in addiction studies, as it is closely linked to cognitive distortions in GD by encompassing impulsive choices, motor responses, decision-making, and cognitive biases. Also, emotions, mood, temperament, and affective state are crucial in developing and maintaining GD. Gambling can be used as a maladaptive coping strategy to avoid or escape problems and distress. Methods: The aim of the present study is to explore differences in personality traits and emotion regulation of people suffering from GD, substance-dependent gamblers (SDGs), and healthy controls (HCs). Additionally, the study proposes a new experimental task: the “Gambling Affective Task” (GAT) to investigate the influence of affective priming on risk-taking behaviors. Results: Our findings indicate that participants placed lower bets following positive priming. Additionally, SDGs wagered significantly higher amounts than HCs, regardless of priming type. In general, participants exhibited longer response times after positive priming trials, compared to negative and neutral priming trials. These findings suggest that experiencing positive emotions can act as a protective factor by delaying and lengthening gambling behaviors. By comparing gamblers with and without substance comorbidity, we can gain insight into the exclusive factors of GD and improve our understanding of this disorder. Conclusions: By elucidating the impact of emotional states on risk-taking, the research also provides new insights into the prevention and treatment of GD. Full article