Search Results (8)

Modern mobility faces increasing challenges, like carbon-free transportation and the need for flexible transportation solutions. The U-Shift II project addresses these problems through a modular electric vehicle architecture, a drive unit (Driveboard) and a vehicle body (Capsule). This separation offers high flexibility in different use cases. Current architecture paradigms, like AUTOSAR, face limitations in cost and development speed. To address these issues, this paper introduces a hybrid software architecture that integrates signal-oriented architecture (e.g., CAN bus) with service-oriented architecture for enhanced flexibility. A integral component of the hybrid architecture is the dynamic link system, which bridges these architectures by dynamically integrating Capsule-specific components into the Driveboard software stack during runtime. The performance of the developed systen and its functionality were evaluated using a hardware setup integrated into a Driveboard prototype. The dynamic link aystem was evaluated including latency measurements, as well as functionality tests. Additionally, a cloud-based reconfiguration process enhances the versatility of the Driveboard by allowing for over-the-air software updates and resource allocation. The results show a promising hybrid, reconfigurable E/E architecture that aims to enable a robust transition towards a pure service-oriented architecture required in future electric autonomous vehicles. Full article

This paper introduces a novel portable multi-layer capsule-shaped triboelectric generator (CP-TEG), aimed at optimizing the performance of triboelectric generator technology in terms of miniaturization, modularity, and efficient energy collection. The CP-TEG utilizes a unique multi-layer, stacked structure and an elliptical cylindrical design to increase the effective frictional area and enhance power generation efficiency. Its portable design allows for flexible application in various environments and scenarios. Experimental results demonstrate that the CP-TEG can maintain stable and efficient electrical output under various motion amplitudes and frequencies, and it shows good adaptability to the direction of motion excitation. With a motion amplitude of 7 cm and a frequency of 1.94 Hz, the CP-TEG can charge a 220 μF capacitor to 1.3 V within 100 s. The power generation unit’s output voltage and current are more than three times higher than that of traditional single-layer contact-separation mode triboelectric devices. Particularly, its performance in harvesting energy from human motion underscores its effectiveness as a renewable energy solution for wearable devices. Through its innovative structural design and optimized working mechanism, the CP-TEG demonstrates excellent energy collection efficiency and application potential, offering new options for sustainable energy solutions and development. Full article

Novel vehicle concepts are needed to meet the requirements of resource-conserving and efficient mobility in the future, especially in urban areas. In the automated, driverless electric vehicle concept U-Shift, a new form of mobility is created by separating a vehicle into a drive module and a transport capsule. The autonomous driving module, the so-called Driveboard, is able to change the transport capsules independently and is therefore used to transport both people and goods. The wide range of possible capsules poses major challenges for the development of the Driveboard and the chassis in particular. A lifting actuator integrated into the chassis concept enables levelling and, thus, the raising and lowering of the Driveboard and the capsules to ground level. This means that no additional lifting devices are required for changing the capsules or for lowering them to the ground, e.g., for loading and unloading the capsules. To realise this mechanism simply and efficiently, a fully electromechanical actuator is designed and constructed. The actuator consists primarily of a profile rail guide, a steel cable winch, an electric motor, a housing that connects the subsystems and a locking mechanism. The electric motor is used to lift the vehicle and regulate the weight force-driven lowering of the vehicle. This paper describes the design of the actuator and shows the dimensioning of all main components according to the boundary conditions. Finally, the prototype model of the realised concept is presented. Full article

The rising antimicrobial resistance is particularly alarming for Acinetobacter baumannii, calling for the discovery and evaluation of alternatives to treat A. baumannii infections. Some bacteriophages produce a structural protein that depolymerizes capsular exopolysaccharide. Such purified depolymerases are considered as novel antivirulence compounds. We identified and characterized a depolymerase (DpoMK34) from Acinetobacter phage vB_AbaP_PMK34 active against the clinical isolate A. baumannii MK34. In silico analysis reveals a modular protein displaying a conserved N-terminal domain for anchoring to the phage tail, and variable central and C-terminal domains for enzymatic activity and specificity. AlphaFold-Multimer predicts a trimeric protein adopting an elongated structure due to a long α-helix, an enzymatic β-helix domain and a hypervariable 4 amino acid hotspot in the most ultimate loop of the C-terminal domain. In contrast to the tail fiber of phage T3, this hypervariable hotspot appears unrelated with the primary receptor. The functional characterization of DpoMK34 revealed a mesophilic enzyme active up to 50 °C across a wide pH range (4 to 11) and specific for the capsule of A. baumannii MK34. Enzymatic degradation of the A. baumannii MK34 capsule causes a significant drop in phage adsorption from 95% to 9% after 5 min. Although lacking intrinsic antibacterial activity, DpoMK34 renders A. baumannii MK34 fully susceptible to serum killing in a serum concentration dependent manner. Unlike phage PMK34, DpoMK34 does not easily select for resistant mutants either against PMK34 or itself. In sum, DpoMK34 is a potential antivirulence compound that can be included in a depolymerase cocktail to control difficult to treat A. baumannii infections. Full article

As wireless capsule endoscope (WCE) technology has advanced, various studies were published on WCEs with functional modules for the diagnosis and treatment of problems in the digestive system. However, when additional functional modules are added the physical size of the WCEs will increase, making them more difficult for patients to comfortably swallow. Moreover, there are limitations when it comes to adding multi-functional modules to the WCEs due to the size of the digestive tract itself. This article introduces a controllable modular capsule endoscope driven by an electromagnetic actuation (EMA) system. The modular capsules are divided into a driving capsule and a functional capsule. Capsules with different functions are swallowed in sequence and then recombination, transportation and separation functions are carried out under the control of the EMA system while in the stomach, this approach solves the size limitation issues faced by multi-functional capsule endoscopes. The recombination and separation functions make use of a characteristic of soft magnetic materials so that their magnetization direction can be changed easily. These functions are made possible by the addition of a soft magnet to the capsule together with the precise control of magnetic fields provided by the EMA system. Full article

Bacteriophages exert strong evolutionary pressure on their microbial hosts. In their lytic lifecycle, complete bacterial subpopulations are utilized as hosts for bacteriophage replication. However, during their lysogenic lifecycle, bacteriophages can integrate into the host chromosome and alter the host’s genomic make-up, possibly resulting in evolutionary important adjustments. Not surprisingly, bacteria have evolved sophisticated immune systems to protect against phage infection. Streptococcus pyogenes isolates are frequently lysogenic and their prophages have been shown to be major contributors to the virulence of this pathogen. Most S. pyogenes phage research has focused on genomic prophages in relation to virulence, but little is known about the defensive arsenal of S. pyogenes against lytic phage infection. Here, we characterized Phage A1, an S. pyogenes bacteriophage, and investigated several mechanisms that S. pyogenes utilizes to protect itself against phage predation. We show that Phage A1 belongs to the Siphoviridae family and contains a circular double-stranded DNA genome that follows a modular organization described for other streptococcal phages. After infection, the Phage A1 genome can be detected in isolated S. pyogenes survivor strains, which enables the survival of the bacterial host and Phage A1 resistance. Furthermore, we demonstrate that the type II-A CRISPR-Cas system of S. pyogenes acquires new spacers upon phage infection, which are increasingly detectable in the absence of a capsule. Lastly, we show that S. pyogenes produces membrane vesicles that bind to phages, thereby limiting the pool of phages available for infection. Altogether, this work provides novel insight into survival strategies employed by S. pyogenes to combat phage predation. Full article

Three new heterometallic [Cr^III₈Ni^II₆] coordination cubes of formulae [Cr^III₈Ni^II₆L₂₄(H₂O)₁₂](NO₃)₁₂ (1), [Cr^III₈Ni^II₆L₂₄(MeCN)₇(H₂O)₅](ClO₄)₁₂ (2), and [Cr^III₈Ni^II₆L₂₄Cl₁₂] (3) (where HL = 1-(4-pyridyl)butane-1,3-dione), were synthesised using the paramagnetic metalloligand [Cr^IIIL₃] and the corresponding Ni^II salt. The magnetic skeleton of each capsule describes a face-centred cube in which the eight Cr^III and six Ni^II ions occupy the eight vertices and six faces of the structure, respectively. Direct current magnetic susceptibility measurements on (1) reveal weak ferromagnetic interactions between the Cr^III and Ni^II ions, with J_Cr-Ni = + 0.045 cm⁻¹. EPR spectra are consistent with weak exchange, being dominated by the zero-field splitting of the Cr^III ions. Excluding wheel-like structures, examples of large heterometallic clusters containing both Cr^III and Ni^II ions are rather rare, and we demonstrate that the use of metalloligands with predictable bonding modes allows for a modular approach to building families of related polymetallic complexes. Compounds (1)–(3) join the previously published, structurally related family of [M^III₈M^II₆] cubes, where M^III = Cr, Fe and M^II = Cu, Co, Mn, Pd. Full article

Today commercial transport in urban areas faces major challenges. These include making optimal use of limited space, avoiding empty trips, meeting driver shortages as well as reducing costs and emissions such as CO₂, particulate matter and noise. The mutual acceleration and reinforcement of technological trends such as electrification, digitization and automation may enable new vehicle and mobility concepts that can meet these challenges. One possible vehicle concept is presented in this article. It is based on on-the-road modularization, i.e., a vehicle that can change different transport capsules during operation. The vehicle is divided into an electrically propelled autonomous drive unit and a transport unit. Standardized interfaces between these units enable the easy design of capsules for different uses, while the drive unit can be used universally. Business models and operating strategies that allow optimal use of this vehicle concept are discussed in depth in the article. First, the current situation is analyzed followed by a detailed description of an exemplary business model using a business model canvas. The operating strategies and logistics concepts are illustrated and compared with conventional concepts. Full article