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12 pages, 1617 KiB  
Article
Genomic Analysis of Reproductive Trait Divergence in Duroc and Yorkshire Pigs: A Comparison of Mixed Models and Selective Sweep Detection
by Changyi Chen, Yu He, Juan Ke, Xiaoran Zhang, Junwen Fei, Boxing Sun, Hao Sun and Chunyan Bai
Vet. Sci. 2025, 12(7), 657; https://doi.org/10.3390/vetsci12070657 - 11 Jul 2025
Viewed by 358
Abstract
This study aimed to investigate population genetic differences related to reproductive traits between Duroc and Yorkshire (Dutch Large White) pigs using two approaches: linear mixed models that dissect additive and dominant effects, and selective sweep analysis. (1) Methods: Genome-wide single-nucleotide polymorphism (SNP) data [...] Read more.
This study aimed to investigate population genetic differences related to reproductive traits between Duroc and Yorkshire (Dutch Large White) pigs using two approaches: linear mixed models that dissect additive and dominant effects, and selective sweep analysis. (1) Methods: Genome-wide single-nucleotide polymorphism (SNP) data of 3917 Duroc and 3217 Yorkshire pigs were analyzed. The first principal component (PC1) was used as a simulated phenotype to capture population-level variance. Additive and dominant genetic effects were partitioned and evaluated by using the combination of the linear mixed models (LMM) and ADDO’s algorithm (LMM + ADDO). In parallel, selective sweep signals were detected using fixation index (FST) and nucleotide diversity (θπ) analyses. A comparative assessment was then conducted between the LMM + ADDO and the selective sweep analysis results. Significant loci were annotated using quantitative trait loci (QTL) databases and the Ensembl genome browser. (2) Results: There are 39040 SNPs retained after quality control. Using the LMM + ADDO framework with PC1 as a simulated phenotype, a total of 632 significant SNPs and 184 candidate genes were identified. Notably, 587 SNPs and 171 genes were uniquely detected by the LMM + ADDO method and not among loci detected by the top 5% of FST and θπ values. Key candidate genes associated with litter size included HSPG2, KAT6B, SAMD8, and LRMDA, while DLGAP1, MYOM1, and VTI1A were associated with teat number traits. (3) Conclusions: This study demonstrates the power of integrating additive and dominant effect modeling with population genetics approaches for the detection of genomic regions under selection. The findings provide novel insights into the genetic architecture of reproductive traits in pigs and have practical implications for understanding the inheritance of complex traits. Full article
(This article belongs to the Special Issue Future Perspectives in Pig Reproductive Biotechnology)
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20 pages, 1461 KiB  
Article
Cu and Pb Co-Contamination Accelerates the Decomposition Rate of Litter from Invasive Aquatic Plant Eichhornia crassipes (Mart.) Solms and the Effect Increases with Its Invasion Degree
by Yizhuo Du, Yingsheng Liu, Xiaoxuan Geng, Yue Li, Chuang Li, Yulong Zhang, Congyan Wang and Daolin Du
Horticulturae 2025, 11(7), 768; https://doi.org/10.3390/horticulturae11070768 - 2 Jul 2025
Viewed by 241
Abstract
Invasive and native plants can coexist in the same ecosystem. Thus, the fallen leaves of invasive and native plants can be mixed, which can lead to co-decomposition. Invasive plants can create microenvironmental conditions conducive to their invasion process by influencing soil physicochemical properties, [...] Read more.
Invasive and native plants can coexist in the same ecosystem. Thus, the fallen leaves of invasive and native plants can be mixed, which can lead to co-decomposition. Invasive plants can create microenvironmental conditions conducive to their invasion process by influencing soil physicochemical properties, soil nutrient contents, and soil enzymatic activities through litter decomposition by released metabolites. Heavy metal contamination may affect the litter decomposition of invasive plants. This study was designed to elucidate the effects of the mono- and co-decomposition of the leaves of the invasive aquatic plant Eichhornia crassipes (Mart.) Solms (Common Water Hyacinth) and the native aquatic plant Nymphaea tetragona Georgi (Pygmy Water-Lily) on soil physicochemical properties, soil nutrient contents, and soil enzymatic activities under the mono- and co-contamination of Cu and Pb. This study was conducted over a six-month period using a polyethylene litter bag experiment. The type of heavy metals may be the most significant factor influencing the differences in the decomposition rate between E. crassipes and N. tetragona. The co-contamination of Cu and Pb increased the decomposition rate of the leaves of E. crassipes and the decomposition rate also increased as the invasion degree of E. crassipes increased relative to N. tetragona. The co-decomposition of the leaves of the two aquatic plants showed an antagonistic response under the mono-contamination of Pb and the control, but presented a synergistic response under the mono-contamination of Cu and the co-contamination of Cu and Pb, regardless of the invasion degree of E. crassipes. Soil enzymatic activities, especially the activities of polyphenol oxidase and cellulase, may be a significant factor influencing the litter decomposition of the two aquatic plants. Consequently, heavy metal contamination may affect the invasion process of E. crassipes with regard to the regulation of the released metabolites during the decomposition process, and this is specifically modulated by the type of heavy metals. Full article
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22 pages, 2625 KiB  
Article
Leaf Litter Mixtures in Guam: Decomposition Synergism and Antagonism of Two Endangered Tree Species
by Thomas E. Marler
Ecologies 2025, 6(3), 47; https://doi.org/10.3390/ecologies6030047 - 1 Jul 2025
Viewed by 567
Abstract
Leaf litter traits among tree species exert a direct influence on spatiotemporal nutrient turnover and an indirect influence by shifting the decomposition dynamics of leaf litter mixtures including other sympatric species. Cycas micronesica and Serianthes nelsonii are two Mariana Island tree species that [...] Read more.
Leaf litter traits among tree species exert a direct influence on spatiotemporal nutrient turnover and an indirect influence by shifting the decomposition dynamics of leaf litter mixtures including other sympatric species. Cycas micronesica and Serianthes nelsonii are two Mariana Island tree species that are endangered, and developing a greater understanding of the influence of these trees on biogeochemistry may improve information-based conservation decisions. The objectives of this study were to quantify the influence of mixing the leaf litter of these species with 12 sympatric forest plants to determine the additive and nonadditive influences on decomposition. The C. micronesica litter was collectively antagonistic when litter mixtures were incubated in a mesocosm study and a field litterbag study, and the response was similar among the included species. The S. nelsonii litter was collectively synergistic among the same mixed species, and the response was dissimilar among the included species. The contributions of these two threatened tree species to spatiotemporal diversity in biogeochemistry are dissimilar and considerable. These findings indicate that species recovery efforts for these two species are of paramount importance for maintaining Mariana Island ecological integrity and native biodiversity by sustaining their contributions to ecosystem services. Full article
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23 pages, 3927 KiB  
Article
Effects of the Light-Felling Intensity on Hydrological Processes in a Korean Pine (Pinus koraiensis) Forest on Changbai Mountain in China
by Qian Liu, Zhenzhao Zhou, Xiaoyang Li, Xinhai Hao, Yaru Cui, Ziqi Sun, Haoyu Ma, Jiawei Lin and Changcheng Mu
Forests 2025, 16(7), 1050; https://doi.org/10.3390/f16071050 - 24 Jun 2025
Viewed by 218
Abstract
(1) Background: Understanding how forest management practices regulate hydrological cycles is critical for sustainable water resource management and addressing global water crises. However, the effects of light-felling (selective thinning) on hydrological processes in temperate mixed forests remain poorly understood. This study comprehensively evaluated [...] Read more.
(1) Background: Understanding how forest management practices regulate hydrological cycles is critical for sustainable water resource management and addressing global water crises. However, the effects of light-felling (selective thinning) on hydrological processes in temperate mixed forests remain poorly understood. This study comprehensively evaluated the impacts of light-felling intensity levels on three hydrological layers (canopy, litter, and soil) in mid-rotation Korean pine (Pinus koraiensis) forests managed under the “planting conifer and preserving broadleaved trees” (PCPBT) system on Changbai Mountain, China. (2) Methods: Hydrological processes—including canopy interception, throughfall, stemflow, litter interception, soil water absorption, runoff, and evapotranspiration—were measured across five light-felling intensity levels (control, low, medium, heavy, and clear-cutting) during the growing season. The stand structure and precipitation characteristics were analyzed to elucidate the driving mechanisms. (3) Results: (1) Low and heavy light-felling significantly increased the canopy interception by 18.9%~57.0% (p < 0.05), while medium-intensity light-felling reduced it by 20.6%. The throughfall was significantly decreased 10.7% at low intensity but increased 5.3% at medium intensity. The stemflow rates declined by 15.8%~42.7% across all treatments. (2) The litter interception was reduced by 22.1% under heavy-intensity light-felling (p < 0.05). (3) The soil runoff rates decreased by 56.3%, 16.1%, and 6.5% under the low, heavy, and clear-cutting intensity levels, respectively, although increased by 27.1% under medium-intensity activity (p < 0.05). (4) The monthly hydrological dynamics shifted from bimodal (control) to unimodal patterns under most treatments. (5) The canopy processes were primarily driven by precipitation, while litter interception was influenced by throughfall and tree diversity. The soil processes correlated strongly with throughfall. (4) Conclusions: Low and heavy light-felling led to enhanced canopy interception and reduced soil runoff and mitigated flood risks, whereas medium-intensity light-felling supports water supply during droughts by increasing the throughfall and runoff. These findings provide critical insights for balancing carbon sequestration and hydrological regulation in forest management. Full article
(This article belongs to the Section Forest Hydrology)
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20 pages, 2190 KiB  
Article
Plastic Pollution and Framework Towards Sustainable Plastic Waste Management in Nigeria: Case Study
by Martha Ogechi Chilote and Hom Nath Dhakal
Environments 2025, 12(6), 209; https://doi.org/10.3390/environments12060209 - 18 Jun 2025
Viewed by 1003
Abstract
Plastic pollution and its environmental consequence are on the rise globally. In Nigeria, the proliferation of plastic bottle and sachet water (PBSW) manufacturing companies in various parts of the country has led to an increase in plastic waste generation. Existing studies have identified [...] Read more.
Plastic pollution and its environmental consequence are on the rise globally. In Nigeria, the proliferation of plastic bottle and sachet water (PBSW) manufacturing companies in various parts of the country has led to an increase in plastic waste generation. Existing studies have identified challenges and the critical need for the adoption of sustainable solutions to mitigate its adverse environmental impact, especially for developing countries. Therefore, the motivation for this study stems from the urgent need for a progressive shift in the studies focused on feasible solutions to the common challenges and strategies for implementation. This study aims to investigate the identified challenges of a lack of awareness and waste management of single-use plastics in Nigeria, towards achieving a circular economy of plastic waste whilst considering its socio-economic context. This study used a mixed method approach combining quantitative and qualitative data through interviews and questionnaires to investigate awareness on the impact of plastic pollution amongst key stakeholders in plastic waste management in the UNN. The potential of introducing a DRS in the sustainable collection of single-use plastic bottle and sachet water waste was also explored. The result reveals the perceived consequence of plastic pollution is short-term, at the level of mesoplastics, physically observed as plastic litter (68.2%), leading to a blockage of canals (65.0%), an excessive rate of flooding (19.1%) and other related issues; effective channels of creating awareness and educating the public on plastic pollution are social media (48.3%), school education (23.3%), mass media (21.7%), and others (6%). An implementation framework for sustainable plastic waste collection was developed from the research findings, adapting the Norwegian Deposit Return Scheme (DRS) to suit the current socio-economic context of the population. Additionally, awareness can be increased through targeted government policies that reward sustainable plastic waste management practices, public awareness campaigns, and the use of social media. Full article
(This article belongs to the Special Issue Editorial Board Members’ Collection Series: Plastic Contamination)
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18 pages, 526 KiB  
Article
The Impact of Social Media Activities on Marine and Coastal Litter in Cyprus
by Kemal Emirzade and Mehmet Fatih Huseyinoglu
Sustainability 2025, 17(11), 4821; https://doi.org/10.3390/su17114821 - 23 May 2025
Viewed by 528
Abstract
This study explores the role of social media in raising awareness, fostering community engagement, and supporting efforts to reduce marine and coastal litter in Cyprus. Focusing on campaigns led by community-based organizations such as Yesil Baris Hareketi, Teneke Cocuk, and the Spot Turtle [...] Read more.
This study explores the role of social media in raising awareness, fostering community engagement, and supporting efforts to reduce marine and coastal litter in Cyprus. Focusing on campaigns led by community-based organizations such as Yesil Baris Hareketi, Teneke Cocuk, and the Spot Turtle Project, the research highlights how digital platforms can mobilize public opinion and influence pro-environmental behaviors. Artificial intelligence (AI) algorithms particularly natural language processing (NLP) techniques were employed to analyze large volumes of social media data, enabling the detection of engagement patterns, sentiment dynamics, and thematic trends within environmental campaigns. A mixed-methods approach was adopted, combining social media content analysis, engagement metrics, and stakeholder interviews to provide a comprehensive view of the digital advocacy landscape. Findings suggest that social media plays a critical role in shaping public perceptions of marine litter; however, sustaining long-term behavioral change remains a significant challenge. The study offers practical recommendations for enhancing digital strategies, strengthening stakeholder collaboration, and integrating social media efforts with policy development and environmental education. Full article
(This article belongs to the Special Issue Innovative Research Methods for Sustainable Educational Development)
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17 pages, 2210 KiB  
Article
Exploring Microbial Diversity in Forest Litter-Based Fermented Bioproducts and Their Effects on Tomato (Solanum lycopersicum L.) Growth in Senegal
by Alexandre Mahougnon Aurel Zoumman, Paula Fernandes, Mariama Gueye, Clémence Chaintreuil, Laurent Cournac, Aboubacry Kane and Komi Assigbetse
Int. J. Plant Biol. 2025, 16(2), 55; https://doi.org/10.3390/ijpb16020055 - 23 May 2025
Viewed by 458
Abstract
Reducing the use of chemical inputs (fertilizers, pesticides) in agriculture while maintaining crop productivity is the main challenge facing sub-Saharan African family farming systems. The use of effective microorganisms (EM) is among the various innovative approaches for minimizing chemical inputs and the environmental [...] Read more.
Reducing the use of chemical inputs (fertilizers, pesticides) in agriculture while maintaining crop productivity is the main challenge facing sub-Saharan African family farming systems. The use of effective microorganisms (EM) is among the various innovative approaches for minimizing chemical inputs and the environmental impact of agricultural production and protecting soil health while enhancing crop yields and improving food security. This study sought to characterize the microbial biodiversity of local beneficial microorganisms (BMs) products from locally fermented forest litter and investigate their ability to enhance tomato plant growth and development. Beneficial microorganisms (BMs) were obtained by anaerobic fermentation of forest litter collected in four agroecological regions of Senegal mixed with sugarcane molasses and various types of carbon sources (groundnut shells, millet stovers, and rice bran in different proportions). The microbial community composition was analyzed using next-generation rDNA sequencing, and their effects on tomato growth traits were tested in greenhouse experiments. Results show that regardless of the litter geographical collection site, the dominant bacterial taxa in the BMs belonged to the phyla Firmicutes (27.75–97.06%) and Proteobacteria (2.93–72.24%). Within these groups, the most prevalent classes were Bacilli (14.41–89.82%), α-proteobacteria (2.83–72.09%), and Clostridia (0.024–13.34%). Key genera included Lactobacillus (13–65.83%), Acetobacter (8.91–72.09%), Sporolactobacillus (1.40–43.35%), and Clostridium (0.08–13.34%). Fungal taxa were dominated by the classes Leotiomycetes and Sordariomycetes, with a prevalence of the acidophilic genus Acidea. Although microbial diversity is relatively uniform across samples, the relative abundance of microbial taxa is influenced by the litter’s origin. This is illustrated by the PCoA analysis, which clusters microbial communities based on their litter source. Greenhouse experiments revealed that five BMs (DK-M, DK-G, DK-GM, NB-R, and NB-M) significantly (p < 0.05) enhanced tomato growth traits, including plant height (+10.75% for DK-G and +9.44% for NB-R), root length (+56.84–62.20%), root volume (+84.32–97.35%), root surface area (+53.16–56.72%), and both fresh and dry shoot biomass when compared to untreated controls. This study revealed that forest-fermented litter products (BMs), produced using litter collected from various regions in Senegal, contain beneficial microorganisms known as plant growth-promoting microorganisms (PGPMs), which enhanced tomato growth. These findings highlight the potential of locally produced BMs as an agroecological alternative to inorganic inputs, particularly within Senegal’s family farming systems. Full article
(This article belongs to the Section Plant–Microorganisms Interactions)
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13 pages, 1580 KiB  
Article
Effects of Mixed Addition of Fraxinus mandshurica Rupr. and Larix gmelinii (Rupr.) Kuzen. Litter on Nitrogen Mineralization in Dark Brown Soil of Northeast China
by Shixing Han, Xuesong Miao, Yandong Zhang and Hailong Sun
Forests 2025, 16(5), 842; https://doi.org/10.3390/f16050842 - 19 May 2025
Viewed by 367
Abstract
The changes in soil nitrogen mineralization rate induced by litter input can determine the availability of nitrogen for plant growth in the soil. In forest ecosystems, the mixing of different species of litter can alter the chemical properties of the litter, ultimately affecting [...] Read more.
The changes in soil nitrogen mineralization rate induced by litter input can determine the availability of nitrogen for plant growth in the soil. In forest ecosystems, the mixing of different species of litter can alter the chemical properties of the litter, ultimately affecting the rates of soil nitrogen transformation and cycling. In this study, litters with Fraxinus mandshurica Rupr. and Larix gmelinii (Rupr.) Kuzen. and mixed litter with Fraxinus mandshurica and Larix gmelinii were added to dark brown soil and incubated in the lab for 175 days at 25 °C. NH4+-N and NO3-N contents and nitrogen mineralization rates were periodically measured to explore the effect of mixed litter addition on soil nitrogen mineralization. The results showed that compared to Larix gmelinii litter, Fraxinus mandshurica litter demonstrates higher carbon, nitrogen, and phosphorus contents while exhibiting lower lignin and cellulose contents and lower C/N and lignin/N ratios. Soil inorganic nitrogen content showed a trend of initial decrease followed by an increase. At the end of the incubation, soil NH4+-N and NO3-N and the total inorganic nitrogen contents were 4.6–7.8 times, 2.2–3.4 times, and 2.9–4.3 times higher than the initial value, respectively. The soil nitrogen mineralization rate exhibited an initial rapid increase followed by stabilization. During days 7–28 of incubation, the nitrogen mineralization rates in litter addition treatments were lower than that in the control, while they were higher than that in the control during days 42–175. The soil nitrogen mineralization rate in the treatments with Fraxinus mandshurica litter and mixed litter were higher than those in the treatment with Larix gmelinii litter. The cumulative net nitrogen mineralization amounts in the Fraxinus mandshurica litter and mixed litter treatments were higher than those in the Larix gmelinii litter treatment, being 1.5 and 1.2 times those of the Larix gmelinii litter treatment, respectively. MBC and MBN presented a trend of first increasing and then decreasing, peaking on days 7 and 14 of incubation, respectively. Correlation analysis revealed that soil inorganic nitrogen content and nitrogen mineralization rate were positively correlated with the litter total nitrogen and soil microbial carbon and nitrogen and negatively correlated with litter C/N and lignin/N. The changes in soil inorganic nitrogen and nitrogen mineralization are primarily associated with soil microbial immobilization. Initially, in the treatments with litter addition, an increase in microbial biomass enhanced the immobilization of soil inorganic nitrogen. Subsequently, as litter mineralization progressed, the amount of litter decreased, leading to reduced microbial biomass and weakened immobilization. This study indicates that the interaction between litter types and soil microorganisms is the key factor affecting soil nitrogen mineralization process and soil mineral nitrogen content. These findings provide a scientific basis for soil fertility management in the forest ecosystems of Northeast China. Full article
(This article belongs to the Special Issue Forest Soil Microbiology and Biogeochemistry)
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21 pages, 4707 KiB  
Article
The Impact of Litter from Different Belowground Organs of Phragmites australis on Microbial-Mediated Soil Organic Carbon Accumulation in a Lacustrine Wetland
by Chong Chen, Yong Wang, Liu Yang, Yongen Min, Keming Yue, Sitong Lu, Hongfeng Bian, Xue Wang and Leilei Zhang
Microorganisms 2025, 13(5), 1146; https://doi.org/10.3390/microorganisms13051146 - 16 May 2025
Viewed by 434
Abstract
Although belowground litter decomposition critically influences lacustrine wetland soil carbon dynamics, the organ-specific microbial mechanisms driving soil organic carbon (SOC) accumulation remain unclear. Existing research has predominantly focused on aboveground litter, leaving a significant gap in the understanding of how roots and rhizomes [...] Read more.
Although belowground litter decomposition critically influences lacustrine wetland soil carbon dynamics, the organ-specific microbial mechanisms driving soil organic carbon (SOC) accumulation remain unclear. Existing research has predominantly focused on aboveground litter, leaving a significant gap in the understanding of how roots and rhizomes differentially regulate carbon cycling through microbial community assembly and survival strategies. This study took Phragmites australis (a plant characteristic of lacustrine wetland) as the research object and examined how decomposing belowground litter from different organs affects microbial-mediated SOC accumulation through a one-year in situ field incubation in Jingyuetan National Forest Park, Changchun City, Jilin Province, China. Our findings reveal that root litter exhibited the highest decomposition rate, which was accelerated by intermittent flooding, reaching up to 1.86 times that of rhizome. This process enriched r-strategist microbial taxa, intensified homogeneous selection, and expanded niche width, directly promoting SOC accumulation. Rhizome litter decomposition enhanced dispersal limitation, promoted K-strategist microbial dominance, and indirectly modulated SOC through soil acidification. Mixed-litter treatments significantly enhanced SOC accumulation (up to three times higher than single-litter treatments) through synergistic nutrient release (non-additive effects < 0.04) and reinforced microbial network interactions. SOC accumulation varied significantly with the flooding regime as follows: non-flooded > intermittent flooding > permanent flooding. This study provides new insights into the microbially driven mechanisms of plant-organ-specific decomposition in the carbon cycling of wetland ecosystems. Full article
(This article belongs to the Section Environmental Microbiology)
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27 pages, 7991 KiB  
Article
Estimation of the Total Carbon Stock of Dudles Forest Based on Satellite Imagery, Airborne Laser Scanning, and Field Surveys
by Kornél Czimber, Botond Szász, Norbert Ács, Dávid Heilig, Gábor Illés, Diána Mészáros, Gábor Veperdi, Bálint Heil and Gábor Kovács
Forests 2025, 16(3), 512; https://doi.org/10.3390/f16030512 - 14 Mar 2025
Cited by 2 | Viewed by 665
Abstract
We present our carbon stock estimation method developed for mixed coniferous and deciduous forests in the Hungarian hilly region, covering diverse site conditions. The method consists of four complex steps, integrating traditional field surveys with modern remote sensing and GIS. The first step [...] Read more.
We present our carbon stock estimation method developed for mixed coniferous and deciduous forests in the Hungarian hilly region, covering diverse site conditions. The method consists of four complex steps, integrating traditional field surveys with modern remote sensing and GIS. The first step involves comprehensive field data collection at systematically distributed sampling points. The second step is tree species mapping based on satellite image time series. The third step uses Airborne Laser Scanning to estimate aboveground biomass and derive the carbon stock of roots. The final step involves evaluating and spatially extending field and laboratory data on litter and humus from sampling points using geostatistical methods, followed by aggregating the results for the forest block and individual forest sub-compartments. New elements were developed and implemented into the complex methodology, such as aboveground biomass calculation with voxel aggregation and underground carbon stock spatial extension with EBK regression prediction. Additionally, we examined how the accuracy of our method, designed for a 200 m sampling grid, decreases as the distance between sampling points increases. Full article
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16 pages, 449 KiB  
Article
Supplementation with Tributyrin for Gestating Sows Reduces Stillborn Rate and Increases Litter Birth Weight
by Flávio de Aguiar Coelho, Ana Ligia Braga Mezzina, Ana Clara Rodrigues de Oliveira, Laya Kannan Silva Alves, Nadia de Almeida Ciriaco Gomes, Jorge Yair Perez-Palencia, Marli Arena Dionizio, Eduardo Machado Costa Lima, Soraia Viana Ferreira and Cesar Augusto Pospissil Garbossa
Vet. Sci. 2025, 12(3), 260; https://doi.org/10.3390/vetsci12030260 - 11 Mar 2025
Viewed by 1055
Abstract
This study evaluated the performance of gestating and lactating sows supplemented with tributyrin from gestation until the end of lactation. Additionally, it assessed the effects of this supplementation on pig growth performance during the nursery phase. A total of 148 commercial-line sows (DB90 [...] Read more.
This study evaluated the performance of gestating and lactating sows supplemented with tributyrin from gestation until the end of lactation. Additionally, it assessed the effects of this supplementation on pig growth performance during the nursery phase. A total of 148 commercial-line sows (DB90 Brasil; parity 1–4; initial weight: 201.7 ± 12.2 kg) were used during gestation and lactation, along with 180 weaned pigs. A randomized block design was utilized with two dietary treatments during gestation and lactation, and a 2 × 2 factorial arrangement during the nursery phase. Data on sow body composition and productivity during gestation and lactation, as well as piglet performance during nursery, were collected. Treatment effects and interactions were analyzed using ANOVA with the MIXED procedure of SAS Version 9.0 (SAS Inst. Inc., Cary, NC, USA). Sows fed tributyrin tended to weigh 3.05 kg more (p = 0.053) and had 6.51% less backfat accumulation prepartum (p = 0.099). Supplementation reduced stillborns by 35.47% (p = 0.032) and increased litter weight at birth by 10.14% (p = 0.018). Additionally, there was a trend toward an 18.73% reduction in weight variability (p = 0.053) with more piglets weighing ≥1.4 kg. Sow and litter performance during lactation were not affected. Piglets from sows supplemented with tributyrin showed a trend for higher feed intake immediately post-weaning (p = 0.056). Tributyrin supplementation during gestation reduced stillborn rates and increased litter weight at birth. Full article
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18 pages, 3108 KiB  
Article
Longitudinal Study on the Effect of Season and Weather on the Behaviour of Domestic Cats (Felis catus)
by Michelle Smit, Christopher J. Andrews, Ina Draganova, Rene A. Corner-Thomas and David G. Thomas
Animals 2025, 15(5), 637; https://doi.org/10.3390/ani15050637 - 22 Feb 2025
Viewed by 1842
Abstract
To date, little is known about seasonal changes in specific cat behaviours, and how these are affected by weather patterns. Using accelerometer data and a validated machine learning model, behaviours including being active, eating, grooming, littering, lying, scratching, sitting, and standing were quantified [...] Read more.
To date, little is known about seasonal changes in specific cat behaviours, and how these are affected by weather patterns. Using accelerometer data and a validated machine learning model, behaviours including being active, eating, grooming, littering, lying, scratching, sitting, and standing were quantified for seven research cats for a total of 13 weeks spread over one year, with weather data being collected simultaneously. Generalised linear mixed models were used to statistically test for seasonal differences in proportional behavioural data and how behaviour was affected by weather variables. Seasonal differences were found for time spent eating (p < 0.001), grooming (p < 0.001), littering (p = 0.037), lying (p < 0.001), scratching (p < 0.001), and sitting (p < 0.001) but not for active behaviours and standing (p > 0.05). A positive interaction effect of the temperature humidity wind index and daylength was found for time spent eating, lying, and standing (all p < 0.001), while it was negative for active (p < 0.001), grooming (p < 0.001), littering (p = 0.004), and sitting (p < 0.001). Rainfall negatively affected grooming (p = 0.023) and scratching (p = 0.037). These findings highlight seasonality in cat behaviours, influenced by weather conditions. Full article
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12 pages, 1218 KiB  
Article
Microbial Diversity Drives Decomposition More than Advantage of Home Environment—Evidence from a Manipulation Experiment with Leaf Litter
by Masoud M. Ardestani, Jaroslav Kukla, Tomáš Cajthaml, Petr Baldrian and Jan Frouz
Microorganisms 2025, 13(2), 351; https://doi.org/10.3390/microorganisms13020351 - 6 Feb 2025
Viewed by 1020
Abstract
Microbial diversity plays a crucial role in litter decomposition. However, the relationships between microbial diversity and substrate successional stage are the drivers of this decomposition. In this study, we experimentally manipulated microbial diversity and succession in post-mining soil. We used leaf litter samples [...] Read more.
Microbial diversity plays a crucial role in litter decomposition. However, the relationships between microbial diversity and substrate successional stage are the drivers of this decomposition. In this study, we experimentally manipulated microbial diversity and succession in post-mining soil. We used leaf litter samples from two forests of a post-mining site near Sokolov, Czech Republic: one alder plantation and one mixed forest with birch aspen and willow. Litter from each site was decomposed in the field for 3 and 12 months. The litter was X-ray sterilized and part of the litter was kept unsterilized to produce inoculum. Leaf litter samples of two different ages (3 and 12 months) from each site were each inoculated with litter of two different ages (3 and 12 months), using less and more diluted inoculum, producing two levels of microbial diversity. In each of these eight treatments, the bacterial community was then characterized by amplicon sequencing of the 16S rRNA gene and microbial respiration was used to assess the rate of decomposition. A significantly higher respiration (p < 0.05) was found for the litter inoculated with the higher level of microbial diversity. Higher respiration was also found for the younger litter compared to the older litter and both litter origins. This shows a reduction in microbial respiration with substrate age and inoculation diversity, suggesting that microbial diversity supports the decomposition of soil organic matter. Full article
(This article belongs to the Section Environmental Microbiology)
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13 pages, 3704 KiB  
Article
Physical Properties and Rheological Characteristics of Cigarette Butt-Modified Asphalt Binders
by Xinhe Hu, Xianglong Chen, Jie Yu, Gang Cheng, Yunxiao Yuan and Lizhou Zhang
Coatings 2025, 15(2), 170; https://doi.org/10.3390/coatings15020170 - 3 Feb 2025
Cited by 2 | Viewed by 801
Abstract
Cigarette butt (CB) waste is abundant and difficult to biodegrade, which is dangerous for both the environment and human health. The key reason CBs are littered is that people do not know much about the harm CBs pose to the environment. Recycling CBs [...] Read more.
Cigarette butt (CB) waste is abundant and difficult to biodegrade, which is dangerous for both the environment and human health. The key reason CBs are littered is that people do not know much about the harm CBs pose to the environment. Recycling CBs in infrastructure construction can help raise people’s awareness. To promote the recycling of CB waste, this paper aimed to determine the feasibility of using CBs as a modifier for asphalt binders. In this research, CBs were preprocessed and mixed with virgin asphalt binder as a fiber modifier. Comprehensive laboratory investigations, including a softening point test, viscosity test, storage stability test, and temperature sweep test, were performed, along with a frequency sweep test, to evaluate the performance of the modified samples. During this investigation, samples were prepared with 1%, 2%, 3%, and 4% CBs. The results of the CB-modified samples were compared with the sample consisting of fresh bitumen (0% fiber). The results show that the physical and rheological properties of bitumen with incorporated CBs improved significantly, and CBs could be used instead of virgin cellulose fiber as a fiber modifier. However, CB-modified asphalt reduced the storage stability and low-temperature performance of the samples. Further research should focus on improving the storage stability and low-temperature performance of CB-modified asphalt binders to facilitate their application in asphalt pavements. Full article
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18 pages, 5849 KiB  
Article
Growth, Productivity, and Nutrient Return of a Mixed Plantation of Fast-Growing Eucalyptus Hybrid and Acacia auriculiformis Trees in Thailand
by Jetsada Wongprom, Narinthorn Jumwong, Pattama Sangvisitpirom, Sapit Diloksumpun and La-ongdao Thaopimai
Forests 2025, 16(1), 182; https://doi.org/10.3390/f16010182 - 19 Jan 2025
Viewed by 1598
Abstract
Mixed-species plantations involving Eucalyptus and Acacia trees are an effective alternative for managing sustainable plantations. In this study, we evaluated the growth, productivity, nutrient return, and soil properties of a mixed Eucalyptus hybrid (Eucalyptus camaldulensis Dehnh. × E. urophylla S.T. Blake; E) and [...] Read more.
Mixed-species plantations involving Eucalyptus and Acacia trees are an effective alternative for managing sustainable plantations. In this study, we evaluated the growth, productivity, nutrient return, and soil properties of a mixed Eucalyptus hybrid (Eucalyptus camaldulensis Dehnh. × E. urophylla S.T. Blake; E) and Acacia auriculiformis A. Cunn. ex Benth. plantation (A) and Eucalyptus hybrid and A. auriculiformis plantations. The mixed Eucalyptus hybrid and A. auriculiformis plantation included three ratios at E33:A67, E50:A50, and E67:A33, while the Eucalyptus (E100) and A. auriculiformis (A100) plantations were established on degraded lands in the Had Wanakorn Forestry Research and Student Training Station, Prachuap Khiri Khan province, Thailand. Three replications within a plot size of 20 × 20 m2 were designed to plant Eucalyptus hybrid and A. auriculiformis seedlings at a spacing of 2 × 3 m2. The diameters at breast height (DBH) and height (H) of the Eucalyptus hybrid and A. auriculiformis were measured and monitored after planting for five years. The aboveground biomass of the five-year-old mixed and monoculture plantations was then estimated. Litterfall production and nutrient return from the mixed and monoculture plantations were measured for three years. In addition, soil samples at depths of 0–5, 5–10, and 10–20 cm were collected to analyze the soil’s chemical properties. Differences in growth, aboveground biomass, litterfall production, nutrient return, and soil properties were analyzed and tested using Tukey’s HSD. The results indicated that both the DBH and H of the Eucalyptus hybrid in the mixed and monoculture plantations were not significantly different (p > 0.05). Similarly, the DBH and H of A. auriculiformis in each treatment were also not significantly different (p > 0.05). However, the DBH and H of the Eucalyptus hybrid were higher than those of A. auriculiformis. The aboveground biomass for the mixed plantation ratios E50:A50, E100, E67:A33, and E33:A67 was not significantly different, while the stem biomass was the highest in E100. Litterfall production was influenced by the proportion of the Eucalyptus hybrid relative to A. auriculiformis, but the monoculture A100 plantation had the highest litter production. The nitrogen return estimated for the mixed plantation was between A100 and E100. Similarly, the total nitrogen in the topsoil (0–5 cm) of the mixed plantation was higher than that in the monoculture E100 plantation. These results indicate that mixing A. auriculiformis with Eucalyptus can improve soil nutrients and nutrient cycling and increase nutrient returns, suggesting that mixed plantations are an effective option for sustainable plantation management and can mitigate the negative environmental impacts of Eucalyptus monocultures. Full article
(This article belongs to the Special Issue Forest Stand and Biomass Management)
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