Search Results (120)

Microgravity (µg)-generated three-dimensional (3D) multicellular aggregates can serve as models of tissue and disease development. They are relevant in the fields of cancer and in vitro metastasis or regenerative medicine (tissue engineering). Driven by the 3R concept—replacement, reduction, and refinement of animal testing—µg-exposure of human cells represents a new alternative method that avoids animal experiments entirely. New Approach Methodologies (NAMs) are used in biomedical research, pharmacology, toxicology, cancer research, radiotherapy, and translational regenerative medicine. Various types of human cells grow as 3D spheroids or organoids when exposed to µg-conditions provided by µg simulating instruments on Earth. Examples for such µg-simulators are the Rotating Wall Vessel, the Random Positioning Machine, and the 2D or 3D clinostat. This review summarizes the most recent literature focusing on µg-engineered tissues. We are discussing all reports examining different tumor cell types from breast, lung, thyroid, prostate, and gastrointestinal cancers. Moreover, we are focusing on µg-generated spheroids and organoids derived from healthy cells like chondrocytes, stem cells, bone cells, endothelial cells, and cardiovascular cells. The obtained data from NAMs and µg-experiments clearly imply that they can support translational medicine on Earth. Full article

Lava tubes on Earth provide unique hydrogeological niches for life to proliferate. Orbital observations of the Martian surface indicate the presence of lava tubes, which could hold the potential for extant life or the preservation of past life within a subsurface environment protected from harsh conditions or weathering at the surface. Secondary minerals in lava tubes form as a combination of abiotic and biotic processes. Microbes colonize the surfaces rich in these secondary minerals, and their actions induce further alteration of the mineral deposits and host basalts. We conducted a biogeochemical investigation of basaltic lava tubes in the Medicine Lake region of northern California by characterizing the compositional variations in secondary minerals, organic compounds, microbial communities, and the host rocks to better understand how their biogeochemical signatures could indicate habitability. We used methods applicable to landed Mars missions, including Raman spectroscopy, X-ray diffraction (XRD), Laser-Induced Breakdown Spectroscopy (LIBS), and gas chromatography–mass spectrometry (GC-MS), along with scanning electron microscopy (SEM) and metagenomic DNA/RNA sequencing. The main secondary minerals, amorphous silicates, and calcite, formed abiotically from the cave waters. Two types of gypsum, large euhedral grains with halites, and cryptocrystalline masses near microbial material, were observed in our samples, indicating different formation pathways. The cryptocrystalline gypsum, along with clay minerals, was associated with microbial materials and biomolecular signatures among weathered primary basalt minerals, suggesting that their formation was related to biologic processes. Some of the genes and pathways observed indicated a mix of metabolisms, including those involved in sulfur and nitrogen cycling. The spatial relationships of microbial material, Cu-enriched hematite in the host basalts, and genetic signatures indicative of metal cycling also pointed to localized Fe oxidation and mobilization of Cu by the microbial communities. Collectively these results affirm the availability of bio-essential elements supporting diverse microbial populations on lava tube basalts. Further work exploring these relationships in lava tubes is needed to unravel the intertwined nature of abiotic and biotic interactions and how that affects habitability in these environments on Earth and the potential for life on Mars. Full article

Few studies have prospectively examined the joint effect of mercury (Hg) and omega-3 long-chain polyunsaturated fatty acids on gestational weight gain (GWG). This exploratory study included 120 women from the Environment and Reproductive Health (EARTH) study with preconception measurements of hair Hg and serum eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), followed through pregnancy. Linear regression was used to examine associations between hair Hg and GWG, while logistic regression estimated the odds of inadequate GWG based on National Academy of Medicine recommendations. In unadjusted models, higher hair Hg (≥1 vs. <1 ppm) was associated with lower GWG (β = −1.89; 95% CI: −3.70, −0.08) and increased odds of insufficient GWG (OR = 2.27; 95% CI: 1.00, 5.18). However, after multivariable adjustment including serum EPA + DHA, these associations were attenuated and became non-significant. A negative, though not statistically significant, association between hair Hg and GWG was observed among women in the lowest tertile of serum EPA + DHA (≥1 vs. <1 ppm: β = −3.26; 95% CI: −7.69, 1.17), whereas no such association was observed among those in higher tertiles (β [95% CI] = 0.44 [−4.21, 5.09] and −1.05 [−4.13, 2.02], respectively). Our findings suggest that the association between preconception Hg exposure and insufficient GWG may differ by serum EPA + DHA, but results require confirmation in cohorts with larger sample sizes. Full article

Addressing the challenge of synergistically optimizing shielding performance and mechanical properties in nuclear radiation shielding materials, this study designed and prepared as-cast Mg-12Dy-xSm-0.4Zr (x = 1, 2, 3) alloys by incorporating rare earth elements Dy and Sm, which possess high thermal neutron absorption cross-sections. The co-addition of Sm and Dy significantly refined the grains and promoted the precipitation of bone-like Mg₅(Sm,Dy) and Mg₄₁Sm₅ phases along grain boundaries. The alloys exhibited favorable mechanical properties, with ultimate tensile strength (UTS) reaching up to 194.6 MPa and elongation (EL) up to 10.9%. However, higher Sm content led to an increased amount of secondary phases at grain boundaries, resulting in stress concentration and a subsequent decline in both yield strength and elongation. Moreover, the combined addition of Dy and Sm markedly enhanced the thermal neutron shielding performance. Experimental results agreed well with Geant4 simulations, showing that both the neutron shielding rate and linear attenuation coefficient improved with increasing Sm content, demonstrating the positive role of Dy and Sm in neutron absorption. The developed alloy achieves simultaneous improvement in mechanical properties and neutron shielding capacity, providing valuable insights for the development of lightweight “function–structure integrated” radiation shielding materials for applications such as nuclear medicine and aerospace. Full article

Despite notable progress in cancer therapy, immune evasion remains a major obstacle to effective treatment outcomes. In the context of spaceflight, astronauts are exposed to unique environmental stressors—particularly microgravity and radiation—that profoundly affect cellular and immune homeostasis. Emerging evidence suggests that microgravity alters key cellular processes, including proliferation, apoptosis, adhesion, and oncogenic signaling pathways such as NF-κB and ERK1/2. Concurrently, microgravity (µg) disrupts immune regulation, potentially facilitating both tumor progression and treatment resistance. Of particular concern is the upregulation of human endogenous retroviruses (HERVs), especially HERV-K and HERV-W, under µg conditions, which may exacerbate inflammatory responses and immune system dysregulation. While some studies indicate that µg may impair tumor growth, others reveal enhanced immune evasion and reduced antitumor immunity. Importantly, insights from µg research extend beyond space medicine and provide translational opportunities for terrestrial oncology, including the development of physiologically relevant 3D tumor models for drug screening, the identification of mechano-sensitive pathways (FAK/RhoA, YAP/TAZ) as therapeutic targets, and novel immunotherapeutic strategies involving epigenetic modulation and checkpoint inhibition. This review critically examines the dual role of µg in modulating cancer progression and immune function. We synthesize findings on how µg shapes immune responses, alters tumor–immune system interactions, and impacts the efficacy of immunotherapeutic approaches. Finally, we highlight translational opportunities and challenges for optimizing cancer immunotherapy and precision oncology in both spaceflight and Earth-based environments. Full article

Silicon has a striking similarity to carbon and is found in plant cells. However, there is no specific role that has been assigned to silicon in the life cycle of plants. The amount of silicon in plant cells is species specific and can reach levels comparable to macronutrients. Silicon is used extensively in artificial intelligence, nanotechnology, and the digital revolution, and thus can serve as an informational molecule such as nucleic acids. The diverse potential of silicon to bond with different chemical species is analogous to carbon; thus, it can serve as a structural candidate similar to proteins. The discovery of large amounts of silicon on Mars and the moon, along with the recent development of enzyme that can incorporate silicon into organic molecules, has propelled the theory of creating silicon-based life. The bacterial cytochrome has been modified through directed evolution such that it could cleave silicon–carbon bonds in organo-silicon compounds. This consolidates the idea of utilizing silicon in biomolecules. In this article, the potential of silicon-based life forms has been hypothesized, along with the reasoning that autotrophic virus-like particles could be used to investigate such potential. Such investigations in the field of synthetic biology and astrobiology will have corollary benefits for Earth in the areas of medicine, sustainable agriculture, and environmental sustainability. Full article

Rare-earth elements (REEs), including lanthanides, scandium, and yttrium, are important for advanced technologies such as renewable energy systems, electronics, medical diagnostics, and precision agriculture. Despite their relative crustal abundance, REE extraction is impeded by complex geochemical behavior, dispersed distribution, and environmental challenges. This review presents a comprehensive overview of REE geochemistry, mineralogy, and major deposit types including carbonatites, alkaline igneous rocks, laterites, placer deposits, coal byproducts, and marine sediments. It also highlights the global distribution and economic potential of key REE projects. The integration of machine learning has further enhanced exploration by enabling deposit classification and geochemical modeling, especially in data-limited regions. Environmental and health challenges associated with REE mining, processing, and electronic waste (e-waste) recycling are studied, along with the expanding use of REEs in agriculture and medicine. Some recycling efforts offer promise for supply diversification, but significant technological and economic barriers remain. Ensuring a secure and sustainable REE supply will require integrated approaches combining advanced analytics, machine learning, responsible extraction, and coordinated policy efforts. The present review offers a general overview that can be useful for informing future studies and resource-related discussions. Full article

Locametz^®/Illuccix^®/Gozellix^TM (Novartis AG (Basel, Switzerland) and Telix Pharmaceuticals, Ltd. (Melbourne, Australia), all three [⁶⁸Ga]Ga-PSMA-11), Pylarify^®/Pylclari^® (Progenics Pharmaceuticals, Inc. (New York, USA) and Curium PET France SA (Paris, France), both [¹⁸F]DCFPyL), Radelumin^® (ABX GmbH (Radeberg, Germany), [¹⁸F]PSMA-1007), and Posluma^® (Blue Earth Diagnostics, Ltd. (Oxford, UK), [¹⁸F]rhPSMA-7.3) are four approved PSMA-PET imaging agents that have significantly advanced the diagnosis and management of prostate cancer. These agents offer a new level of precision and accuracy, enabling clinicians to detect prostate cancer with enhanced sensitivity. As a result, they play a critical role in improving detection, staging, and management, ultimately enhancing clinical outcomes for patients. Their use in routine clinical practice is expected to increase diagnostic precision and provide clearer pathways for personalized therapy. This review offers a comprehensive chemical, pharmaceutical, and medicinal overview, discusses comparative studies, and highlights additional highly relevant candidates for prostate cancer detection. Full article

Metals are natural components of the lithosphere, whose amounts and bioavailability are increasing in many areas due to their continuous release from both natural sources and intensive human activities. Some metals are essential or beneficial for living organisms, while others are non-essential and potentially toxic. When present at higher concentrations, even essential and beneficial metal ions can become harmful to all forms of life. Bacteria, unicellular organisms that have been exposed to metals since the earliest stages of life on Earth, have evolved metabolic pathways involving essential metals as well as diverse strategies to cope with metal toxicity. In the domain Bacteria, two main strategies have been identified: (i) metal exclusion, which includes cell wall sequestration and immobilization of metals in extracellular exopolysaccharides, siderophores, and other soluble microbial products, as well as (ii) metal tolerance, involving intracellular sequestration of metals (e.g., by metallothioneins, or low molecular weight thiols) as well as enzymatic conversion of metals to less toxic forms and/or its active efflux. Microorganisms possessing such adaptive traits are considered valuable agents for potential application in medicine, environmental sciences, and bioengineering (e.g., bioremediation and/or biomining). Full article

Marigold (Tagetes erecta L.), a crop of significant medicinal, ornamental, and economic value, faces severe industrialization challenges due to weed-induced yield losses (up to 60%). This study aims to identify safe and highly efficient herbicides for marigold, assess their effects on dominant weeds and crop safety, and provide a practical basis for large-scale cultivation. We evaluated 11 pre-emergence herbicides, 13 post-emergence herbicides, and agronomic practices (plastic mulch) through three field trials to optimize weed control, crop safety, and productivity. In Experiment 1, pre-emergence applications of pendimethalin (35% SC) and oxyfluorfen (240 g/L EC) under plastic mulch suppressed 85–99% of grass and broad-leaved weeds, elevating marigold yield to 1655.6 kg/667 m² and increasing lutein content by 10.7% compared to controls, with no phytotoxicity to subsequent wheat (Triticum aestivum L.)or broad beans (Vicia faba L.). Experiment 2 demonstrated that post-cultivation soil treatment with metolachlor · oxyfluorfen · pendimethalin (50% EC) enhanced weed suppression (47.8–53.6%) and yield (3.4% increase) while ensuring crop safety. Experiment 3 revealed that the post-emergence herbicides haloxyfop-P-methyl (108 g/L EC) and fomesafen (250 g/L SL) achieved over 92% reduction in grass weed biomass and over 75% reduction in broadleaf weed density, respectively, alongside a 6.1% yield improvement. Therefore, region-specific strategies are recommended based on local agronomic conditions: high-value production zones should adopt integrated systems combining plastic mulch with pre-emergence herbicides; arid lands with extended crop rotation intervals require pre-emergence herbicides after intertillage and earthing-up; labor-abundant regions can rotate targeted post-emergence herbicides to delay resistance evolution. This study provides data-driven optimization strategies for comprehensive weed management in marigold fields, offering practical solutions to enhance industrial productivity and ecological sustainability. Full article

Virginia mallow or Sida hermaphrodita (L.) Rusby (SH) is a perennial plant from the Malvaceae family (mallows) that is used for medicinal purposes, reducing soil erosion, cleaning soil, and most recently for energy production. The potential of sustainable lignocellulosic agro-waste is immense as it represents Earth’s most abundant organic compound. This paper explores fibers isolated from SH stems, a plant with significant industrial application potential, including technical textiles and biocomposites. The fibers were harvested in January, March, and November of 2020 and in January and March of 2021, and their yield, mechanical properties, moisture content, and density were thoroughly analyzed. The fiber yield showed slight variations depending on the harvest time, with consistent results observed across different years, suggesting stable productivity. The SH fibers demonstrated a favorable moisture content, making them suitable for storage and processing, and their density ranged between 1.52 and 1.58 g/cm³, comparable to that of other natural fibers. According to this research, the best mechanical properties were observed in the winter harvest. Furthermore, the high percentage of solid residue left after fiber extraction shows promise for sustainable utilization, primarily for biofuel production. This study underscores the versatility and sustainability of SH fibers, positioning them as a valuable resource for a wide range of industrial applications. Full article

The cultivation of Moringa oleifera Lam. (M. oleifera) has steadily increased over the past few decades, and interest in the crop continues to rise due to its unique multi-purpose properties. However, knowledge pertaining to its water use to guide decision-making in relation to the growth and management of this crop remains fairly limited. Since acquiring such information can be challenging using traditional in situ or remote sensing-based methods, particularly in resource-poor regions, this study aims to explore the potential of using the cloud-based Earth Engine Evapotranspiration Flux (EEFlux) model to quantify the water use of M. oleifera in a semi-arid region of South Africa. For this purpose, EEFlux estimates were acquired and compared with eddy covariance measurements between November 2022 and May 2023. The results of these comparisons demonstrated that EEFlux unsatisfactorily estimated ET, producing root mean square error, mean absolute error, and R² values of 2.03 mm d⁻¹, 1.63 mm d⁻¹, and 0.24, respectively. The poor performance of this model can be attributed to several factors such as the quantity and quality of the in situ data as well as inherent model limitations. While these results are less than satisfactory, EEFlux affords users a quick and convenient approach to extracting crucial ET and ancillary data. Subsequently, with further refinement and testing, EEFlux can potentially serve to provide a wide variety of users with an invaluable tool to guide and inform decision-making with regards to agricultural water use management, particularly those in resource-constrained environments. Full article

Chelidonium majus L. is a species with a wide medicinal use, commonly found in anthropogenically degraded habitats, forest edges, and urban parks. This study aimed to determine the chemical composition of the leaves, stems, and roots of Ch. majus and the soil in its rhizosphere in terms of the content of the main elements (Fe, Ca, P, Mg, Al, Na, K, S), trace elements and rare earth minerals (Ti, Mo, Ag, U, Au, Th, Sb, Bi, V, La, B, W, Sc, Tl, Se, Te, Ga, Cs, Ge, Hf, Nb, Rb, Sn, Ta, Zr, Y, Ce, In, Be, and Li), and their comparison in the parts analyzed. The study was conducted in five urban parks in southern Poland in a historically industrialized area. The results showed that Ca has the highest content among the macroelements. Its leaf content ranges from 24,700 to 40,700 mg·kg⁻¹, while in soil, it ranges from 6500 to 15,000 mg·kg⁻¹. In leaves, low values of Al (100–500 mg·kg⁻¹) and Na (100 mg·kg⁻¹) were found in comparison to the other elements tested, while high values of Al (5100–9800 mg·kg⁻¹) were found in soils. Among the macroelements in the Ch. majus stems, K showed the highest concentration (>100,000 mg·kg⁻¹), while the Ca content was 3–4 times lower in the stems than in the leaves. Rhizomes of Ch. majus accumulate the most K and Ca, in the range of 22,800–29,900 mg·kg⁻¹ and 5400–8900 mg·kg⁻¹, respectively. Fe and Al in all locations have higher values in the soil than in the tissues. In turn, the content of Ca, P, Mg, K, and S is higher in plants than in the soil. Determining the elemental content of medicinal plants is important information, as the plant draws these elements from the soil, and, at higher levels of toxicity, it may indicate that the plant should not be taken from this habitat for medicinal purposes. Full article

Moringa oleifera (LAM) is a multipurpose tree species with extensive pharmacological and ethnomedicinal properties. Production of important medicinal plants is facing decline under changing climatic conditions, which brings along exacerbated abiotic stresses like salinity and intraspecific competition, particularly high planting densities. Increasing plant density is seen as a strategy to increase production; however, the intraspecific competition and a lack of arable land limit productivity. Salinity has been estimated to harm approximately six percent of the Earth’s landmass. This leads to a loss of over 20% of agricultural output annually. These stressors can significantly curtail moringa’s growth and yield potential. Literature designates that Arbuscular Mycorrhizal Fungi (AMF), ubiquitous soil microorganisms forming symbiotic associations with plant roots, offer a promising avenue for mitigating these stresses. This narrative review aims to investigate the utilization of AMF to alleviate the detrimental effects of salinity and high planting density on Moringa oleifera. The different adaptive strategies M. oleifera undergoes to mitigate both stressors are explored. The review found that AMF inoculation enhances plant tolerance to these stressors by improving nutrient acquisition, water relations, and activating stress response mechanisms. By facilitating improved nutrient and water absorption, AMF enhance root architecture, modulate ROS scavenging mechanisms, and promote optimal biomass allocation, ensuring better survival in high-density plantings. Furthermore, AMF-mediated stress alleviation is linked to enhanced physiological efficiency, including increased chlorophyll content, root–shoot biomass balance, and ion homeostasis. This review is important because it could provide insights into a sustainable, natural solution for improving the resilience of Moringa oleifera under adverse environmental conditions, with potential applications in global agriculture and food security. Future research should prioritize identifying and characterizing moringa-specific AMF species and evaluate the long-term efficacy, feasibility, and economic viability of AMF application in real-world moringa cultivation systems to fully harness the potential of AMF in moringa cultivation. Full article

Parasitic diseases pose a serious threat to the health of humans and the steady development of livestock husbandry. Although there are certain drug-based treatment methods, with the widespread application of drugs, various parasites are gradually developing drug resistance. Natural products are highly favored by researchers due to their characteristics such as low toxicity, multi-target effects, and low risk of drug resistance. The ocean, as the largest treasure trove of biological resources on Earth, has a special ecosystem (high pressure, high salt, and low oxygen). This enables marine organisms to develop a large number of unique structures during their survival competition. So far, a variety of compounds, such as terpenoids, have been isolated from the algae. As potential drugs, these compounds have certain curative effects on various diseases, including tumors, parasitic diseases, Alzheimer’s disease, and tuberculosis. This paper systematically reviews and analyzes the current advances in research on the antiparasite effects of seaweed extracts. The primary objective of this research is to formulate a conceptual foundation for marine pharmaceutical exploration, focusing on the creation of innovative marine-based medicinal compounds to overcome the emerging problem of parasite resistance to conventional treatments. Full article