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14 pages, 280 KiB  
Article
Effects of Dietary Puffed Jujube Powder on Growth Performance, Apparent Digestibility, and Meat Quality of Hainan Black Goats
by Yi Zhang, Jianzhi Shi, Jiapeng Wang, Keke Li, Xianzheng Qiao, Dong Chen, Tingting Dong, Yuanxiao Li, Yushu Zhang and Renlong Lv
Animals 2025, 15(15), 2306; https://doi.org/10.3390/ani15152306 - 6 Aug 2025
Abstract
This study was conducted to investigate the effects of puffed jujube powder (PJP) supplementation in the diet on the slaughter characteristics, growth performance, meat quality, and serum antioxidant capacity of Hainan Black (HB) goats. Twenty-four healthy male HB goats, three months old with [...] Read more.
This study was conducted to investigate the effects of puffed jujube powder (PJP) supplementation in the diet on the slaughter characteristics, growth performance, meat quality, and serum antioxidant capacity of Hainan Black (HB) goats. Twenty-four healthy male HB goats, three months old with an initial body weight of 15.12 ± 3.67 kg, were randomly divided into three groups: the 10% PJP group (basal diet plus 10% PJP); the 20% PJP group (basal diet plus 20% PJP); and the control group (basal diet only). After a 10-day adaptation period, a feeding trial was carried out for 90 days in an ad libitum diet environment. The results show that the final body weight of the 20% PJP group was markedly higher (p < 0.05) than that of the control group (22.58 ± 0.94 kg vs. 20.45 ± 1.01 kg). The average daily gain of the 20% PJP group was 83.44 ± 1.78 g/d, which was substantially greater (p < 0.05) than the 59.22 ± 2.13 g/d of the control group. The feed intake of the 20% PJP group was 713.10 ± 4.54 g/d, notably higher (p < 0.05) than the 498.20 ± 4.33 g/d of the control group. In terms of slaughter characteristics, the carcass weight of the 20% PJP group was 13.99 ± 1.22 kg, considerably heavier (p < 0.05) than the 11.79 ± 1.38 kg of the control group. The muscle weight of the 20% PJP group was 11.43 ± 1.42 kg, distinctly greater (p < 0.05) than the 9.59 ± 1.99 kg of the control group. The slaughter rate of the 20% PJP group was 42.41%, showing a notable increase (p < 0.05) compared with the 37.42% of the control group, and the net meat rate of the 20% PJP group was 34.65%, with a significant rise (p < 0.05) compared with the 30.43% of the control group. Regarding serum antioxidant capacity and meat quality, the activities of serum antioxidant enzymes, superoxide dismutase (SOD) and catalase (CAT), were conspicuously increased (p < 0.05) in the 20% PJP group. The meat shear force of the 20% PJP group was decreased by 12.9%, and the cooking loss was improved by 8.9% in comparison with the control group. In conclusion, the supplementation of 20% PJP in the diet was demonstrated to enhance the growth performance, improve the meat quality, and boost the antioxidant status of HB goats, thus presenting a feasible strategy for optimizing tropical goat production systems. Full article
(This article belongs to the Section Animal Nutrition)
17 pages, 1097 KiB  
Review
Natural Feed Additives in Sub-Saharan Africa: A Systematic Review of Efficiency and Sustainability in Ruminant Production
by Zonaxolo Ntsongota, Olusegun Oyebade Ikusika and Thando Conference Mpendulo
Ruminants 2025, 5(3), 36; https://doi.org/10.3390/ruminants5030036 - 6 Aug 2025
Abstract
Ruminant livestock production plays a crucial role in the agricultural systems of Sub-Saharan Africa, significantly supporting rural livelihoods through income generation, improved nutrition, and employment opportunities. Despite its importance, the sector continues to face substantial challenges, such as low feed quality, seasonal feed [...] Read more.
Ruminant livestock production plays a crucial role in the agricultural systems of Sub-Saharan Africa, significantly supporting rural livelihoods through income generation, improved nutrition, and employment opportunities. Despite its importance, the sector continues to face substantial challenges, such as low feed quality, seasonal feed shortages, and climate-related stresses, all of which limit productivity and sustainability. Considering these challenges, the adoption of natural feed additives has emerged as a promising strategy to enhance animal performance, optimise nutrient utilisation, and mitigate environmental impacts, including the reduction of enteric methane emissions. This review underscores the significant potential of natural feed additives such as plant extracts, essential oils, probiotics, and mineral-based supplements such as fossil shell flour as sustainable alternatives to conventional growth promoters in ruminant production systems across the region. All available documented evidence on the topic from 2000 to 2024 was collated and synthesised through standardised methods of systematic review protocol—PRISMA. Out of 319 research papers downloaded, six were included and analysed directly or indirectly in this study. The results show that the addition of feed additives to ruminant diets in all the studies reviewed significantly (p < 0.05) improved growth parameters such as average daily growth (ADG), feed intake, and feed conversion ratio (FCR) compared to the control group. However, no significant (p > 0.05) effect was found on cold carcass weight (CCW), meat percentage, fat percentage, bone percentage, or intramuscular fat (IMF%) compared to the control. The available evidence indicates that these additives can provide tangible benefits, including improved growth performance, better feed efficiency, enhanced immune responses, and superior meat quality, while also supporting environmental sustainability by reducing nitrogen excretion and decreasing dependence on antimicrobial agents. Full article
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20 pages, 346 KiB  
Review
Dietary Strategies in the Prevention of MASLD: A Comprehensive Review of Dietary Patterns Against Fatty Liver
by Barbara Janota, Karolina Janion, Aneta Buzek and Ewa Janczewska
Metabolites 2025, 15(8), 528; https://doi.org/10.3390/metabo15080528 - 4 Aug 2025
Viewed by 298
Abstract
Understanding the components of the diet, food groups, and nutritional strategies that help prevent MASLD (Metabolic Dysfunction-Associated Steatotic Liver Disease) is essential for identifying dietary behaviors that can stop the progression of this condition, which currently affects over one-quarter of the global population. [...] Read more.
Understanding the components of the diet, food groups, and nutritional strategies that help prevent MASLD (Metabolic Dysfunction-Associated Steatotic Liver Disease) is essential for identifying dietary behaviors that can stop the progression of this condition, which currently affects over one-quarter of the global population. This review highlights the importance of including antioxidant nutrients in the diet, such as vitamins C and E, CoQ10, and polyphenolic compounds. It also emphasizes substances that support lipid metabolism, including choline, alpha-lipoic acid, and berberine. Among food groups, it is crucial to choose those that help prevent metabolic disturbances. Among carbohydrate-rich foods, vegetables, fruits, and high-fiber products are recommended. For protein sources, eggs, fish, and white meat are preferred. Among fat sources, plant oils and fatty fish are advised due to their content of omega-3 and omega-6 fatty acids. Various dietary strategies aimed at preventing MASLD should include elements of the Mediterranean diet or be personalized to provide anti-inflammatory compounds and substances that inhibit fat accumulation in liver cells. Other recommended dietary models include the DASH diet, the flexitarian diet, intermittent fasting, and diets that limit fructose and simple sugars. Additionally, supplementing the diet with spirulina or chlorella, berberine, probiotics, or omega-3 fatty acids, as well as drinking several cups of coffee per day, may be beneficial. Full article
(This article belongs to the Special Issue Metabolic Dysregulation in Fatty Liver Disease)
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18 pages, 309 KiB  
Article
Effects of Adding Hydroxytyrosol to the Diet of Pigs in the Nursery Phase on Growth Performance, Biochemical Markers, and Fatty Acid Profile
by Rafael Domingos Augusto Rofino, Cassio Antonio Ficagna, Taeline Zamboni, Bruna Klein, Enrico A. Altieri, Kevin E. O’Connor, Reeta Davis, Margaret Walsh, Fernando de Castro Tavernari, Marcel Manente Boiago, Aleksandro Schafer da Silva and Diovani Paiano
Animals 2025, 15(15), 2268; https://doi.org/10.3390/ani15152268 - 1 Aug 2025
Viewed by 255
Abstract
This study evaluated the effects of dietary hydroxytyrosol (HT) addition on piglets during the nursery phase across two experiments. In the first, 72 weaned male piglets (~26 days old, 7.3 ± 0.5 kg) were assigned to one of four diets containing 0, 5, [...] Read more.
This study evaluated the effects of dietary hydroxytyrosol (HT) addition on piglets during the nursery phase across two experiments. In the first, 72 weaned male piglets (~26 days old, 7.3 ± 0.5 kg) were assigned to one of four diets containing 0, 5, 10, or 50 mg HT/kg feed. Growth performance, serum biochemistry, histological and behavioral parameters, and meat lipid profiles were assessed. In the second study, the apparent digestibility of diets containing 0, 25, or 50 mg HT/kg feed was evaluated using 15 male piglets (21.5 ± 1.5 kg) through total excreta collection. Results revealed that HT influenced serum glucose and gamma-glutamyl transferase, histological inflammation, and active behaviors. HT modified lipid profiles, reduced capric, lauric, linolenic, arachidonic, cis-5,8,11,14,17-eicosapentaenoic fatty acid concentrations, and increased the nervonic acid profile. The digestibility of dry matter, organic matter, energy, and protein increased with HT use up to 50 mg/kg of feed. These findings demonstrate that HT positively impacts piglet efficiency, changing the fatty acid profile with increased nervonic acid, highlighting its potential as a dietary additive for improving nursery pig production. Full article
(This article belongs to the Section Animal Nutrition)
19 pages, 3251 KiB  
Article
Effects of Dietary Cinnamaldehyde Supplementation on Growth Performance, Serum Antioxidant Capacity, Intestinal Digestive Enzyme Activities, Morphology, and Caecal Microbiota in Meat Rabbits
by Dongjin Chen, Yuxiang Lan, Yuqin He, Chengfang Gao, Bin Jiang and Xiping Xie
Animals 2025, 15(15), 2262; https://doi.org/10.3390/ani15152262 - 1 Aug 2025
Viewed by 210
Abstract
Cinnamaldehyde (CA) is a potential substitute for antibiotic growth promoters in animal breeding. In this study, we investigated its effects as a dietary supplement on growth performance, serum antioxidant capacity, intestinal digestive enzyme activities, intestinal morphology, and caecal microbiota in meat rabbits. Weaned [...] Read more.
Cinnamaldehyde (CA) is a potential substitute for antibiotic growth promoters in animal breeding. In this study, we investigated its effects as a dietary supplement on growth performance, serum antioxidant capacity, intestinal digestive enzyme activities, intestinal morphology, and caecal microbiota in meat rabbits. Weaned meat rabbits (n = 450) were randomly assigned to five groups, Groups A, B, C, D, and E, and fed 0, 50, 100, 150, and 200 mg/kg CA diets, respectively, for 47 days. Biological samples including serum (antioxidants), duodenal/caecal content (enzymes), intestinal tissue (morphology), and caecal digesta (microbiota) were collected at day 47 postweaning for analysis. Groups C and D showed significantly higher final body weights than Group A, with Group D (150 mg/kg CA) demonstrating superior growth performance including 11.73% longer duodenal villi (p < 0.05), 28.6% higher microbial diversity (p < 0.01), and 62% lower diarrhoea rate versus controls. Digestive enzyme activity as well as serum antioxidant capacity increased with increasing CA dose, Microbiota analysis revealed CA increased fibre-fermenting Oscillospiraceae (+38%, p < 0.01) while reducing Ruminococcaceae (−27%, p < 0.05). Thus, dietary CA supplementation at 150 mg/kg was identified as the optimal CA dose for improving meat rabbit production. These findings highlight CA as a functional feed additive for promoting sustainable rabbit production. Full article
(This article belongs to the Section Animal Nutrition)
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15 pages, 524 KiB  
Systematic Review
Association Between Maternal Diet and Frequency of Micronuclei in Mothers and Newborns: A Systematic Review
by Anny Cristine de Araújo, Priscila Kelly da Silva Bezerra do Nascimento, Marília Cristina Santos de Medeiros, Raul Hernandes Bortolin, Ricardo Ney Cobucci and Adriana Augusto de Rezende
Nutrients 2025, 17(15), 2535; https://doi.org/10.3390/nu17152535 - 1 Aug 2025
Viewed by 224
Abstract
Background/Objectives: The effect of diet on maternal and infant genetic levels has been reported in the literature. Diet-associated DNA damage, such as the presence of micronuclei (MN), may be related to an increased risk of developing chronic diseases such as cancer. There is [...] Read more.
Background/Objectives: The effect of diet on maternal and infant genetic levels has been reported in the literature. Diet-associated DNA damage, such as the presence of micronuclei (MN), may be related to an increased risk of developing chronic diseases such as cancer. There is particular concern regarding this damage during pregnancy, as it may affect the newborn (NB). Thus, this review aims to summarize the primary evidence of the impact of diet on the frequency of MN in the mother–infant population. Methods: Five databases (PubMed, Embase, Web of Science, Scopus, and ScienceDirect) were used to search for observational studies. Google Scholar and manual searching were required to perform the “gray literature” search. Results: The search strategy retrieved 1418 records. Of these, 13 were read in full and 5 were included in the review. Most studies were of the cohort type (n = 4) and were carried out in the European region. A total of 875 pregnant women and 238 newborns were evaluated. Despite insufficient evidence to confirm that diet changes the frequency of MN, the included studies found possible effects from the consumption of fried red meat and processed meats and the adequate consumption of vegetables and polyunsaturated fats. Conclusions: Future research is needed in order to understand the effects of diet on genetic stability and to obtain evidence to help plan public policies on food and nutrition or reinforce protective dietary patterns for this and future generations. Full article
(This article belongs to the Section Nutrition in Women)
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15 pages, 1391 KiB  
Article
Valorization of Food By-Products: Formulation and Evaluation of a Feed Complement for Broiler Chickens Based on Bonito Fish Meal and Única Potato Peel Flour
by Ashley Marianella Espinoza Davila and Rebeca Salvador-Reyes
Resources 2025, 14(8), 125; https://doi.org/10.3390/resources14080125 - 1 Aug 2025
Viewed by 259
Abstract
Restaurants and open markets generate considerable quantities of organic waste. Converting these residues into poultry feed ingredients offers a sustainable disposal route. This study aimed to evaluate the nutritional and sensory viability of a novel feed complement formulated from Bonito fish meal ( [...] Read more.
Restaurants and open markets generate considerable quantities of organic waste. Converting these residues into poultry feed ingredients offers a sustainable disposal route. This study aimed to evaluate the nutritional and sensory viability of a novel feed complement formulated from Bonito fish meal (Sarda chiliensis chiliensis) and Única potato peel flour (Solanum tuberosum L. cv. Única). This study was conducted in three phases: (i) production and nutritional characterization of the two by-product flours; (ii) formulation of a 48:52 (w/w) blend, incorporated into broiler diets at 15%, 30%, and 45% replacement levels over a 7-week trial divided into starter (3 weeks), grower (3 weeks), and finisher (1 week) phases; and (iii) assessment of growth performance (weight gain, final weight, and feed conversion ratio), followed by a sensory evaluation of the resulting meat using a Check-All-That-Apply (CATA) analysis. The Bonito fish meal exhibited 50.78% protein, while the Única potato peel flour was rich in carbohydrates (74.08%). The final body weights of broiler chickens ranged from 1872.1 to 1886.4 g across treatments, and the average feed conversion ratio across all groups was 0.65. Replacing up to 45% of commercial feed with the formulated complement did not significantly affect growth performance (p > 0.05). Sensory analysis revealed that meat from chickens receiving 15% and 45% substitution levels was preferred in terms of aroma and taste, whereas the control group was rated higher in appearance. These findings suggest that the formulated feed complement may represent a viable poultry-feed alternative with potential sensory and economic benefits, supporting future circular-economy strategies. Full article
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19 pages, 573 KiB  
Article
Dietary Habits and Obesity in Middle-Aged and Elderly Europeans—The Survey of Health, Ageing, and Retirement in Europe (SHARE)
by Manuela Maltarić, Jasenka Gajdoš Kljusurić, Mirela Kolak, Šime Smolić, Branko Kolarić and Darija Vranešić Bender
Nutrients 2025, 17(15), 2525; https://doi.org/10.3390/nu17152525 - 31 Jul 2025
Viewed by 240
Abstract
Background/Objectives: Understanding the impact of dietary habits in terms of obesity, health outcomes, and functional decline is critical in Europe’s growing elderly population. This study analyzed trends in Mediterranean diet (MD) adherence, obesity prevalence, and grip strength among middle-aged and elderly Europeans [...] Read more.
Background/Objectives: Understanding the impact of dietary habits in terms of obesity, health outcomes, and functional decline is critical in Europe’s growing elderly population. This study analyzed trends in Mediterranean diet (MD) adherence, obesity prevalence, and grip strength among middle-aged and elderly Europeans using data from the Survey of Health, Ageing and Retirement in Europe (SHARE). Methods: Data from four SHARE waves (2015–2022) across 28 countries were analyzed. Dietary patterns were assessed through food frequency questionnaires classifying participants as MD-adherent or non-adherent where adherent implies daily consumption of fruits and vegetables and occasional (3–6 times/week) intake of eggs, beans, legumes, meat, fish, or poultry (an unvalidated definition of the MD pattern). Handgrip strength, a biomarker of functional capacity, was categorized into low, medium, and high groups. Body mass index (BMI), self-perceived health (SPHUS), chronic disease prevalence, and CASP-12 scores (control, autonomy, self-realization, and pleasure evaluated on the 12-item version) were also evaluated. Statistical analyses included descriptive methods, logistic regressions, and multiple imputations to address missing data. Results: A significant majority (74–77%) consumed fruits and vegetables daily, which is consistent with MD principles; however, the high daily intake of dairy products (>50%) indicates limited adherence to the MD, which advocates for moderate consumption of dairy products. Logistic regression indicated that individuals with two or more chronic diseases were more likely to follow the MD (odds ratio [OR] = 1.21, confidence interval [CI] = 1.11–1.32), as were those individuals who rated their SPHUS as very good/excellent ([OR] = 1.42, [CI] = 1.20–1.69). Medium and high maximal handgrip were also strongly and consistently associated with higher odds of MD adherence (Medium: [OR] = 1.44, [CI] = 1.18–1.74; High: [OR] = 1.27, [CI] = 1.10–1.48). Conclusions: The findings suggest that middle-aged and older adults are more likely to adhere to the MD dietary pattern if they have more than two chronic diseases, are physically active, and have a medium or high handgrip. Although an unvalidated definition of the MD dietary pattern was used, the results highlight the importance of implementing targeted dietary strategies for middle-aged and elderly adults. Full article
(This article belongs to the Special Issue Food Insecurity, Nutritional Status, and Human Health)
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18 pages, 1863 KiB  
Article
A Daily Accumulation Model for Predicting PFOS Residues in Beef Cattle Muscle After Oral Exposure
by Ian Edhlund, Lynn Post and Sara Sklenka
Toxics 2025, 13(8), 649; https://doi.org/10.3390/toxics13080649 - 31 Jul 2025
Viewed by 566
Abstract
Per- and polyfluoroalkyl substances (PFAS) have been found worldwide in water, soil, plants, and animals, including humans. A primary route of exposure for humans and animals to PFAS is through the diet and drinking water. Perfluorooctane sulfonate (PFOS), a long-chain PFAS with a [...] Read more.
Per- and polyfluoroalkyl substances (PFAS) have been found worldwide in water, soil, plants, and animals, including humans. A primary route of exposure for humans and animals to PFAS is through the diet and drinking water. Perfluorooctane sulfonate (PFOS), a long-chain PFAS with a relatively long half-life, has been associated with adverse health effects in humans and laboratory animals. There are few toxicokinetic studies on PFOS in domestic livestock raised for human food consumption, which are critical for assessing human food safety. This work aimed to develop a simple daily accumulation model (DAM) for predicting PFOS residues in edible beef cattle muscle. A one-compartment toxicokinetic model in a spreadsheet format was developed using simple calculations to account for daily PFAS into and out of the animal. The DAM was used to simulate two case studies to predict resultant PFOS residues in edible beef cattle tissues. The results demonstrated that the model can reasonably predict PFOS concentrations in beef cattle muscle in a real-world scenario. The DAM was then used to simulate dietary PFOS exposure in beef cattle throughout a typical lifespan in order to derive a generic bioaccumulation factor. The DAM is expected to work well for other PFAS in beef cattle, PFAS in other livestock species raised for meat, and other chemical contaminants with relatively long half-lives. Full article
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15 pages, 288 KiB  
Article
Association of Dietary Sodium-to-Potassium Ratio with Nutritional Composition, Micronutrient Intake, and Diet Quality in Brazilian Industrial Workers
by Anissa Melo Souza, Ingrid Wilza Leal Bezerra, Karina Gomes Torres, Gabriela Santana Pereira, Raiane Medeiros Costa and Antonio Gouveia Oliveira
Nutrients 2025, 17(15), 2483; https://doi.org/10.3390/nu17152483 - 29 Jul 2025
Viewed by 249
Abstract
Introduction: The sodium-to-potassium (Na:K) ratio in the diet is a critical biomarker for cardiovascular and metabolic health, yet global adherence to recommended levels remains poor. Objectives: The objective of this study was to identify dietary determinants of the dietary Na:K ratio and its [...] Read more.
Introduction: The sodium-to-potassium (Na:K) ratio in the diet is a critical biomarker for cardiovascular and metabolic health, yet global adherence to recommended levels remains poor. Objectives: The objective of this study was to identify dietary determinants of the dietary Na:K ratio and its associations with micronutrient intake and diet quality. Methods: An observational cross-sectional survey was conducted in a representative sample of manufacturing workers through a combined stratified proportional and two-stage probability sampling plan, with strata defined by company size and industrial sector from the state of Rio Grande do Norte, Brazil. Dietary intake was assessed using 24 h recalls via the Multiple Pass Method, with Na:K ratios calculated from quantified food composition data. Diet quality was assessed with the Diet Quality Index-International (DQI-I). Multiple linear regression was used to analyze associations of Na:K ratio with the study variables. Results: The survey was conducted in the state of Rio Grande do Norte, Brazil, in 921 randomly selected manufacturing workers. The sample mean age was 38.2 ± 10.7 years, 55.9% males, mean BMI 27.2 ± 4.80 kg/m2. The mean Na:K ratio was 1.97 ± 0.86, with only 0.54% of participants meeting the WHO recommended target (<0.57). Fast food (+3.29 mg/mg per serving, p < 0.001), rice, bread, and red meat significantly increased the ratio, while fruits (−0.16 mg/mg), dairy, white meat, and coffee were protective. Higher Na:K ratios were associated with lower intake of calcium, magnesium, phosphorus, and vitamins C, D, and E, as well as poorer diet quality (DQI-I score: −0.026 per 1 mg/mg increase, p < 0.001). Conclusions: These findings highlight the critical role of processed foods in elevating Na:K ratios and the potential for dietary modifications to improve both electrolyte balance and micronutrient adequacy in industrial workers. The study underscores the need for workplace interventions that simultaneously address sodium reduction, potassium enhancement, and overall diet quality improvement tailored to socioeconomic and cultural contexts, a triple approach not previously tested in intervention studies. Future studies should further investigate nutritional consequences of imbalanced Na:K intake. Full article
(This article belongs to the Special Issue Mineral Nutrition on Human Health and Disease)
28 pages, 1387 KiB  
Article
Metagenomic Analysis of Ready-to-Eat Foods on Retail Sale in the UK Identifies Diverse Genes Related to Antimicrobial Resistance
by Edward Haynes, Roy Macarthur, Marc Kennedy, Chris Conyers, Hollie Pufal, Sam McGreig and John Walshaw
Microorganisms 2025, 13(8), 1766; https://doi.org/10.3390/microorganisms13081766 - 29 Jul 2025
Viewed by 162
Abstract
Antimicrobial Resistance (AMR), i.e., the evolution of microbes to become resistant to chemicals used to control them, is a global public health concern that can make bacterial diseases untreatable. Inputs including antibiotics, metals, and biocides can create an environment in the agrifood chain [...] Read more.
Antimicrobial Resistance (AMR), i.e., the evolution of microbes to become resistant to chemicals used to control them, is a global public health concern that can make bacterial diseases untreatable. Inputs including antibiotics, metals, and biocides can create an environment in the agrifood chain that selects for AMR. Consumption of food represents a potential exposure route to AMR microbes and AMR genes (ARGs), which may be present in viable bacteria or on free DNA. Ready-to-eat (RTE) foods are of particular interest because they are eaten without further cooking, so AMR bacteria or ARGs that are present may be consumed intact. They also represent varied production systems (fresh produce, cooked meat, dairy, etc.). An evidence gap exists regarding the diversity and consumption of ARGs in RTE food, which this study begins to address. We sampled 1001 RTE products at retail sale in the UK, in proportion to their consumption by the UK population, using National Diet and Nutrition Survey data. Bacterial DNA content of sample extracts was assessed by 16S metabarcoding, and 256 samples were selected for metagenomic sequencing for identification of ARGs based on consumption and likely bacterial DNA content. A total of 477 unique ARGs were identified in the samples, including ARGs that may be involved in resistance to important antibiotics, such as colistin, fluoroquinolones, and carbapenems, although phenotypic AMR was not measured. Based on the incidence of ARGs in food types, ARGs are estimated to be present in a high proportion of average diets. ARGs were detected on almost all RTE food types tested (48 of 52), and some efflux pump genes are consumed in 97% of UK diets. Full article
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14 pages, 893 KiB  
Article
Unhealthy Ultra-Processed Food, Diet Quality and Adherence to the Mediterranean Diet in Children and Adolescents: The DELICIOUS Project
by Francesca Giampieri, Alice Rosi, Evelyn Frias-Toral, Osama Abdelkarim, Mohamed Aly, Achraf Ammar, Raynier Zambrano-Villacres, Juancho Pons, Laura Vázquez-Araújo, Nunzia Decembrino, Alessandro Scuderi, Alice Leonardi, Lorenzo Monasta, Fernando Maniega Legarda, Ana Mata, Adrián Chacón, Pablo Busó and Giuseppe Grosso
Foods 2025, 14(15), 2648; https://doi.org/10.3390/foods14152648 - 28 Jul 2025
Viewed by 343
Abstract
Background: Western dietary patterns worldwide are increasingly dominated by energy-dense, nutrient-deficient industrial foods, often identified as ultra-processed foods (UPFs). Such products may have detrimental health implications, particularly if nutritionally inadequate. This study aimed to examine the intake of unhealthy UPFs among children and [...] Read more.
Background: Western dietary patterns worldwide are increasingly dominated by energy-dense, nutrient-deficient industrial foods, often identified as ultra-processed foods (UPFs). Such products may have detrimental health implications, particularly if nutritionally inadequate. This study aimed to examine the intake of unhealthy UPFs among children and adolescents from five Mediterranean countries (Italy, Spain, Portugal, Egypt, and Lebanon) involved in the DELICIOUS project and to assess the association with dietary quality indicators. Methods: A survey was conducted with a sample of 2011 parents of children and adolescents aged 6 to 17 years to evaluate their dietary habits. Diet quality was assessed using the Youth Healthy Eating Index (Y-HEI), the KIDMED index to determine adherence to the Mediterranean diet, and compliance with national dietary guidelines. Results: Increased UPF consumption was not inherently associated with healthy or unhealthy specific food groups, although children and adolescents who consumed UPF daily were less likely to exhibit high overall diet quality and adherence to the Mediterranean diet. In all five countries, greater UPF intake was associated with poorer compliance with dietary recommendations concerning fats, sweets, meat, and legumes. Conclusions: Increased UPF consumption among Mediterranean children and adolescents is associated with an unhealthy dietary pattern, possibly marked by a high intake of fats, sweets, and meat, and a low consumption of legumes. Full article
(This article belongs to the Special Issue Food Habits, Nutritional Knowledge, and Nutrition Education)
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15 pages, 465 KiB  
Article
Ultra-Processed Food Intake as an Effect Modifier in the Association Between Depression and Diabetes in Brazil: A Cross-Sectional Study
by Yunxiang Sun, Poliana E. Correia, Paula P. Teixeira, Bernardo F. Spiazzi, Elisa Brietzke, Mariana P. Socal and Fernando Gerchman
Nutrients 2025, 17(15), 2454; https://doi.org/10.3390/nu17152454 - 28 Jul 2025
Viewed by 738
Abstract
Background/Objectives: Recent studies linked a diet rich in ultra-processed foods (UPFs) with depression and diabetes. Although common risk factors, such as aging, are defined for both diseases, how UPFs are associated with the bidirectional relationship between them is not known. This study aimed [...] Read more.
Background/Objectives: Recent studies linked a diet rich in ultra-processed foods (UPFs) with depression and diabetes. Although common risk factors, such as aging, are defined for both diseases, how UPFs are associated with the bidirectional relationship between them is not known. This study aimed to investigate whether UPF intake modifies the association between depression and diabetes within the Brazilian adult population. Methods: This cross-sectional analysis utilized data from the 2019 Brazilian National Health Survey, involving over 87,000 adults (aged 18–92 years). Participants provided self-reported data on diabetes and depression diagnoses, dietary habits (assessed by qualitative FFQ), as well as demographic, and socioeconomic variables. Multivariate logistic regression models were used to evaluate the associations, employing two classification methods—UPF1 and UPF2—based on different thresholds of weekly consumption, for high/low UPF intake. Analyses were stratified by age groups to identify variations in associations. Results: There was a significant association between depression and diabetes, especially among participants with high UPF consumption. Models adjusted by demographic characteristics, as well as meat and vegetable consumptions, demonstrated elevated odds ratios (ORs) for diabetes among individuals with depression consuming high levels of UPF, compared to those with a low UPF intake (OR: 1.258; 95% CI: 1.064–1.489 for UPF1 and OR: 1.251; 95% CI: 1.059–1.478 for UPF2). Stratified analysis by age further amplified these findings, with younger individuals showing notably stronger associations (non-old adult group OR: 1.596; 95% CI: 1.127–2.260 for UPF1, and OR: 6.726; 95% CI: 2.625–17.233 for UPF2). Conclusions: These findings suggest that high UPF intake may influence the relationship between depression and diabetes, especially in younger adults. Future longitudinal studies are warranted to establish causality, investigate underlying biological mechanisms, and examine whether improving overall nutrient intake through dietary interventions can reduce the co-occurrence of depression and diabetes. Full article
(This article belongs to the Special Issue Ultra-Processed Foods and Chronic Diseases Nutrients)
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16 pages, 1127 KiB  
Article
Effects of Corn–Soybean Meal-Based Fermented Feed Supplementation on Growth Performance, Meat Quality, Fatty Acid Profiles, Nutritional Values, and Gut Microbiota of Lean-Type Finishing Pigs
by Jiao Song, Xin Wang, Yuhan Cao, Yue He and Ye Yang
Foods 2025, 14(15), 2641; https://doi.org/10.3390/foods14152641 - 28 Jul 2025
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Abstract
This research investigated the impact of corn–soybean meal-based fermented feed on the growth performance, pork quality, and fatty acid profiles of lean-type finishing pigs. A total of 80 lean-type growing DLY (Duroc × Landrace–Yorkshire) pigs were randomly assigned to 2 groups, with 5 [...] Read more.
This research investigated the impact of corn–soybean meal-based fermented feed on the growth performance, pork quality, and fatty acid profiles of lean-type finishing pigs. A total of 80 lean-type growing DLY (Duroc × Landrace–Yorkshire) pigs were randomly assigned to 2 groups, with 5 replicates of 8 pigs per pen. The pigs in control group (CON group) were fed a basal diet, while the pigs in fermented feed group (FF group) were fed a diet supplemented with 10% fermented feed. The experimental period lasted 70 days. Results exhibited that pigs in FF group had a significant increase in final body weight and average daily gain (ADG) (p < 0.05) and had a significant decrease in the feed-to-gain ratio (F/G) (p < 0.05). The FF group also exhibited significant promotion in muscle intramuscular fat content, marbling score, and meat color and significantly reduced the meat shear force and drip loss (p < 0.05). Serum analysis indicated that fermented feed significantly elevated blood glucose, total cholesterol, triglyceride levels, and serum hormones such as insulin, leptin, and IGF-1 (p < 0.05). Additionally, fermented feed significantly elevated the levels of polyunsaturated fatty acids (PUFAs) and monounsaturated fatty acids (MUFAs), whereas it decreased the saturated fatty acids (SFAs) contents (p < 0.05). The fermented feed also significantly enhanced pork nutritional values (p < 0.05). The fermented feed increased the expression of IGF-1, SREBP1c, PDE3, PPARγ, SCD5, and FAT/CD36 mRNA (p < 0.05). Furthermore, microbial 16S rDNA analysis uncovered that FF supplementation significantly reduced the Campilobacterota phylum abundance, while increasing the genus abundances of Clostridium_sensu_stricto, norank_f_Oscillospiraceae, unclassified_c_Clostridia, and V9D2013 (p < 0.05). In summary, the results indicated that the microbial fermented feed exhibited the regulation effects on pork quality and nutritional values of lean-type pigs through regulating lipid metabolism and gut microbial composition. Full article
(This article belongs to the Section Food Nutrition)
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19 pages, 2828 KiB  
Review
Microbial Proteins: A Green Approach Towards Zero Hunger
by Ayesha Muazzam, Abdul Samad, AMM Nurul Alam, Young-Hwa Hwang and Seon-Tea Joo
Foods 2025, 14(15), 2636; https://doi.org/10.3390/foods14152636 - 28 Jul 2025
Viewed by 416
Abstract
The global population is increasing rapidly and, according to the United Nations (UN), it is expected to reach 9.8 billion by 2050. The demand for food is also increasing with a growing population. Food shortages, land scarcity, resource depletion, and climate change are [...] Read more.
The global population is increasing rapidly and, according to the United Nations (UN), it is expected to reach 9.8 billion by 2050. The demand for food is also increasing with a growing population. Food shortages, land scarcity, resource depletion, and climate change are significant issues raised due to an increasing population. Meat is a vital source of high-quality protein in the human diet, and addressing the sustainability of meat production is essential to ensuring long-term food security. To cover the meat demand of a growing population, meat scientists are working on several meat alternatives. Bacteria, fungi, yeast, and algae have been identified as sources of microbial proteins that are both effective and sustainable, making them suitable for use in the development of meat analogs. Unlike livestock farming, microbial proteins produce less environmental pollution, need less space and water, and contain all the necessary dietary components. This review examines the status and future of microbial proteins in regard to consolidating and stabilizing the global food system. This review explores the production methods, nutritional benefits, environmental impact, regulatory landscape, and consumer perception of microbial protein-based meat analogs. Additionally, this review highlights the importance of microbial proteins by elaborating on the connection between microbial protein-based meat analogs and multiple UN Sustainable Development Goals. Full article
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