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19 pages, 1363 KiB  
Article
Non-Structural Carbohydrate Concentration Increases and Relative Growth Decreases with Tree Size in the Long-Lived Agathis australis (D.Don) Lindl.
by Julia Kaplick, Benjamin M. Cranston and Cate Macinnis-Ng
Forests 2025, 16(8), 1270; https://doi.org/10.3390/f16081270 (registering DOI) - 3 Aug 2025
Abstract
The southern conifer Agathis australis (D.Don) Lindl. is a large and long-lived species endemic to Aotearoa New Zealand. It is threatened due to past logging activities, pathogen attack and potentially climate change, with increasing severity and frequency of drought and heatwaves across its [...] Read more.
The southern conifer Agathis australis (D.Don) Lindl. is a large and long-lived species endemic to Aotearoa New Zealand. It is threatened due to past logging activities, pathogen attack and potentially climate change, with increasing severity and frequency of drought and heatwaves across its distribution. Like many large tree species, little is known about the carbon dynamics of this ecologically and culturally significant species. We explored seasonal variations in non-structural carbohydrates (NSCs) and growth in trees ranging from 20 to 175 cm diameter at breast height (DBH). NSCs were seasonally stable with no measurable pattern across seasons. However, we found growth rates standardised to basal area and sapwood area (growth efficiency) declined with tree age and stem NSC concentrations (including total NSCs, sugars and starch) all increased as trees aged. Total NSC concentrations were 0.3%–0.6% dry mass for small trees and 0.8%–1.8% dry mass for larger trees, with strong relationships between DBH and total NSC, sugar and starch in stems but not roots. Cumulative growth efficiency across the two-year study period declined as tree size increased. Furthermore, there was an inverse relationship between growth efficiency across the two-year study period and NSC concentrations of stems. This relationship was driven by differences in carbon dynamics in trees of different sizes, with trees progressing to a more conservative carbon strategy as they aged. Simultaneously declining growth efficiency and increasing NSC concentrations as trees age could be evidence for active NSC accumulation to buffer against carbon starvation in larger trees. Our study provides new insights into changing carbon dynamics as trees age and may be evidence for active carbon accumulation in older trees. This may provide the key for understanding the role of carbon processes in tree longevity. Full article
(This article belongs to the Section Forest Ecophysiology and Biology)
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26 pages, 15636 KiB  
Article
Influence of Sample Mass and Pouring Temperature on the Effectiveness of Thermal Analysis for Estimating Gray Iron Inoculation Potential
by Raymundo del Campo-Castro, Manuel Castro-Román, Edgar-Ivan Castro-Cedeno and Martín Herrera-Trejo
Materials 2025, 18(15), 3640; https://doi.org/10.3390/ma18153640 (registering DOI) - 2 Aug 2025
Abstract
Thermal analysis (TA) has been a valuable tool for controlling the carbon equivalent (CE) of cast irons. Additionally, this technique can provide enhanced control over melt quality, allowing for the avoidance of defects such as undesirable graphite morphology and the formation of carbides. [...] Read more.
Thermal analysis (TA) has been a valuable tool for controlling the carbon equivalent (CE) of cast irons. Additionally, this technique can provide enhanced control over melt quality, allowing for the avoidance of defects such as undesirable graphite morphology and the formation of carbides. To obtain the most valuable information from the TA, it is necessary to minimize the variations in the filling operation of the TA cups. However, the mass and pouring temperature of TA cups can vary in TA’s typical foundry operations. A design of experiments was performed to determine whether specific parameters of cooling curves used for quality control can distinguish the inoculation effect in the melt when the mass and the pouring temperature of TA cups are varied. The minimum temperature of the eutectic arrest proved to be a robust inoculation potential control parameter when variations in the cup’s mass were within a range of 268–390 g and were filled at any pouring temperature between 1235 and 1369 °C. Lighter cups under 268 g and poured at a low temperature are not suitable for controlling inoculation potential by TA; however, they remain helpful in controlling CE. These later cups are related to cooling times of less than 180 s, which can serve as a criterion for discarding unsuitable samples. A bimodal population of cell surfaces was revealed in the samples, with the population of small cells being proportionally more numerous in samples with lower TEmin values. Full article
13 pages, 4752 KiB  
Article
The Impact of Harvest Season on Oolong Tea Aroma Profile and Quality
by Chao Zheng, Shuilian Gao, Xiaxia Wang, Zhenbiao Yang, Junling Zhou and Ying Liu
Plants 2025, 14(15), 2378; https://doi.org/10.3390/plants14152378 (registering DOI) - 1 Aug 2025
Viewed by 24
Abstract
The impact of seasonality on the aroma quality of tea has been documented in various tea types, but not specifically in oolong tea. This study is the first to explore the complex relationships between seasonality, volatile compounds, and aroma quality in oolong tea. [...] Read more.
The impact of seasonality on the aroma quality of tea has been documented in various tea types, but not specifically in oolong tea. This study is the first to explore the complex relationships between seasonality, volatile compounds, and aroma quality in oolong tea. Using Headspace Solid-Phase Microextraction Gas Chromatography–Mass Spectrometry (HS-SPME-GC-MS)-based untargeted metabolomics, we analyzed 266 samples of Tieguanyin oolong tea. The data identified linalool, linalool oxides (trans-linalool oxide (furanoid) and trans-linalool oxide (pyranoid)), and their metabolites (diendiol I; hotrienol) as key seasonal discriminants. Four out of the top ten key differential compounds for distinguishing aroma scores were metabolites from fatty acid degradation, namely trans-3-hexenyl butyrate, trans-2-hexenyl hexanoate, hexyl hexanoate, and hexyl 2-methyl butyrate. Approximately one-fifth of the seasonal discriminant volatile compounds were significant in influencing aroma quality. Overall, the impact of seasonality on the aroma quality of finished Tieguanyin oolong tea is marginal. These findings enhance our understanding of the interplay between seasonal variations, volatile composition, and aroma quality in oolong tea. Full article
(This article belongs to the Special Issue Production, Quality and Function of Tea)
36 pages, 3621 KiB  
Review
Harnessing Molecular Phylogeny and Chemometrics for Taxonomic Validation of Korean Aromatic Plants: Integrating Genomics with Practical Applications
by Adnan Amin and Seonjoo Park
Plants 2025, 14(15), 2364; https://doi.org/10.3390/plants14152364 - 1 Aug 2025
Viewed by 219
Abstract
Plant genetics and chemotaxonomic analysis are considered key parameters in understanding evolution, plant diversity and adaptation. Korean Peninsula has a unique biogeographical landscape that supports various aromatic plant species, each with considerable ecological, ethnobotanical, and pharmacological significance. This review aims to provide a [...] Read more.
Plant genetics and chemotaxonomic analysis are considered key parameters in understanding evolution, plant diversity and adaptation. Korean Peninsula has a unique biogeographical landscape that supports various aromatic plant species, each with considerable ecological, ethnobotanical, and pharmacological significance. This review aims to provide a comprehensive overview of the chemotaxonomic traits, biological activities, phylogenetic relationships and potential applications of Korean aromatic plants, highlighting their significance in more accurate identification. Chemotaxonomic investigations employing techniques such as gas chromatography mass spectrometry, high-performance liquid chromatography, and nuclear magnetic resonance spectroscopy have enabled the identification of essential oils and specialized metabolites that serve as valuable taxonomic and diagnostic markers. These chemical traits play essential roles in species delimitation and in clarifying interspecific variation. The biological activities of selected taxa are reviewed, with emphasis on antimicrobial, antioxidant, anti-inflammatory, and cytotoxic effects, supported by bioassay-guided fractionation and compound isolation. In parallel, recent advances in phylogenetic reconstruction employing DNA barcoding, internal transcribed spacer regions, and chloroplast genes such as rbcL and matK are examined for their role in clarifying taxonomic uncertainties and inferring evolutionary lineages. Overall, the search period was from year 2001 to 2025 and total of 268 records were included in the study. By integrating phytochemical profiling, pharmacological evidence, and molecular systematics, this review highlights the multifaceted significance of Korean endemic aromatic plants. The conclusion highlights the importance of multidisciplinary approaches including metabolomics and phylogenomics in advancing our understanding of species diversity, evolutionary adaptation, and potential applications. Future research directions are proposed to support conservation efforts. Full article
(This article belongs to the Special Issue Applications of Bioinformatics in Plant Science)
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21 pages, 2332 KiB  
Article
Evaluation of Spent Catalyst from Fluid Catalytic Cracking in Fly Ash and Blast Furnace Slag Based Alkali Activated Materials
by Yolanda Luna-Galiano, Domigo Cabrera-Gallardo, Mónica Rodríguez-Galán, Rui M. Novais, João A. Labrincha and Carlos Leiva Fernández
Recycling 2025, 10(4), 149; https://doi.org/10.3390/recycling10040149 - 1 Aug 2025
Viewed by 142
Abstract
The objective of this work is to evaluate how spent catalyst from fluid catalytic cracking (SCFCC) affects the physical, mechanical and durability properties of fly ash (FA) and blast furnace slag (BFS)-based alkali-activated materials (AAMs). Recycling of SCFCC by integrating it in a [...] Read more.
The objective of this work is to evaluate how spent catalyst from fluid catalytic cracking (SCFCC) affects the physical, mechanical and durability properties of fly ash (FA) and blast furnace slag (BFS)-based alkali-activated materials (AAMs). Recycling of SCFCC by integrating it in a AAM matrix offers several advantages: valorization of the material, reducing its disposal in landfills and the landfill cost, and minimizing the environmental impact. Mineralogical, physical and mechanical characterization were carried out. The durability of the specimens was studied by performing acid attack and thermal stability tests. Mass variation, compressive strength and porosity parameters were determined to assess the durability. BFS- and FA-based AAMs have a different chemical composition, which contribute to variations in microstructure and physical and mechanical properties. Acid neutralization capacity was also determined to analyse the acid attack results. Porosity, including the pore size distribution, and the acid neutralization capacity are crucial in explaining the resistance of the AAMs to sulfuric acid attack and thermal degradation. Herein, a novel route was explored, the use of SCFCC to enhance the durability of AAMs under harsh operating conditions since results show that the compositions containing SCFCC showed lower strength decay due to the lower macroporosity proportions in these compositions. Full article
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16 pages, 3072 KiB  
Article
Process Development to Repair Aluminum Components, Using EHLA and Laser-Powder DED Techniques
by Adrienn Matis, Min-Uh Ko, Richard Kraft and Nicolae Balc
J. Manuf. Mater. Process. 2025, 9(8), 255; https://doi.org/10.3390/jmmp9080255 (registering DOI) - 31 Jul 2025
Viewed by 149
Abstract
The article presents a new AM (Additive Manufacturing) process development, necessary to repair parts made from Aluminum 6061 material, with T6 treatment. The laser Directed Energy Deposition (DED) and Extreme High-Speed Directed Energy Deposition (EHLA) capabilities are evaluated for repairing Al large components. [...] Read more.
The article presents a new AM (Additive Manufacturing) process development, necessary to repair parts made from Aluminum 6061 material, with T6 treatment. The laser Directed Energy Deposition (DED) and Extreme High-Speed Directed Energy Deposition (EHLA) capabilities are evaluated for repairing Al large components. To optimize the process parameters, single-track depositions were analyzed for both laser-powder DED (feed rate of 2 m/min) and EHLA (feed rate 20 m/min) for AlSi10Mg and Al6061 powders. The cross-sections of single tracks revealed the bonding characteristics and provided laser-powder DED, a suitable parameter selection for the repair. Three damage types were identified on the Al component to define the specification of the repair process and to highlight the capabilities of laser-powder DED and EHLA in repairing intricate surface scratches and dents. Our research is based on variation of the powder mass flow and beam power, studying the influence of these parameters on the weld bead geometry and bonding quality. The evaluation criteria include bonding defects, crack formation, porosity, and dilution zone depth. The bidirectional path planning strategy was applied with a fly-in and fly-out path for the hatching adjustment and acceleration distance. Samples were etched for a qualitative microstructure analysis, and the HV hardness was tested. The novelty of the paper is the new process parameters for laser-powder DED and EHLA deposition strategies to repair large Al components (6061 T6), using AlSi10Mg and Al6061 powder. Our experimental research tested the defect-free deposition and the compatibility of AlSi10Mg on the Al6061 substrate. The readers could replicate the method presented in this article to repair by laser-powder DED/EHLA large Al parts and avoid the replacement of Al components with new ones. Full article
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26 pages, 8845 KiB  
Article
Occurrence State and Genesis of Large Particle Marcasite in a Thick Coal Seam of the Zhundong Coalfield in Xinjiang
by Xue Wu, Ning Lü, Shuo Feng, Wenfeng Wang, Jijun Tian, Xin Li and Hayerhan Xadethan
Minerals 2025, 15(8), 816; https://doi.org/10.3390/min15080816 (registering DOI) - 31 Jul 2025
Viewed by 146
Abstract
The Junggar Basin contains a large amount of coal resources and is an important coal production base in China. The coal seam in Zhundong coalfield has a large single-layer thickness and high content of inertinite, but large particle Fe-sulphide minerals are associated with [...] Read more.
The Junggar Basin contains a large amount of coal resources and is an important coal production base in China. The coal seam in Zhundong coalfield has a large single-layer thickness and high content of inertinite, but large particle Fe-sulphide minerals are associated with coal seams in some mining areas. A series of economic and environmental problems caused by the combustion of large-grained Fe-sulphide minerals in coal have seriously affected the economic, clean and efficient utilization of coal. In this paper, the ultra-thick coal seam of the Xishanyao formation in the Yihua open-pit mine of the Zhundong coalfield is taken as the research object. Through the analysis of coal quality, X-ray fluorescence spectrometer test of major elements in coal, inductively coupled plasma mass spectrometry test of trace elements, SEM-Raman identification of Fe-sulphide minerals in coal and LA-MC-ICP-MS test of sulfur isotope of marcasite, the coal quality characteristics, main and trace element characteristics, macro and micro occurrence characteristics of Fe-sulphide minerals and sulfur isotope characteristics of marcasite in the ultra-thick coal seam of the Xishanyao formation are tested. On this basis, the occurrence state and genesis of large particle Fe-sulphide minerals in the ultra-thick coal seam of the Xishanyao formation are clarified. The main results and understandings are as follows: (1) the occurrence state of Fe-sulphide minerals in extremely thick coal seams is clarified. The Fe-sulphide minerals in the extremely thick coal seam are mainly marcasite, and concentrated in the YH-2, YH-3, YH-8, YH-9, YH-14, YH-15 and YH-16 horizons. Macroscopically, Fe-sulphide minerals mainly occur in three forms: thin film Fe-sulphide minerals, nodular Fe-sulphide minerals, and disseminated Fe-sulphide minerals. Microscopically, they mainly occur in four forms: flake, block, spearhead, and crack filling. (2) The difference in sulfur isotope of marcasite was discussed, and the formation period of marcasite was preliminarily divided. The overall variation range of the δ34S value of marcasite is wide, and the extreme values are quite different. The polyflake marcasite was formed in the early stage of diagenesis and the δ34S value was negative, while the fissure filling marcasite was formed in the late stage of diagenesis and the δ34S value was positive. (3) The coal quality characteristics of the thick coal seam were analyzed. The organic components in the thick coal seam are mainly inertinite, and the inorganic components are mainly clay minerals and marcasite. (4) The difference between the element content in the thick coal seam of the Zhundong coalfield and the average element content of Chinese coal was compared. The major element oxides in the thick coal seam are mainly CaO and MgO, followed by SiO2, Al2O3, Fe2O3 and Na2O. Li, Ga, Ba, U and Th are enriched in trace elements. (5) The coal-accumulating environment characteristics of the extremely thick coal seam are revealed. The whole thick coal seam is formed in an acidic oxidation environment, and the horizon with Fe-sulphide minerals is in an acidic reduction environment. The acidic reduction environment is conducive to the formation of marcasite and is not conducive to the formation of pyrite. (6) There are many matrix vitrinite, inertinite content, clay content, and terrigenous debris in the extremely thick coal seam. The good supply of peat swamp, suitable reduction environment and pH value, as well as groundwater leaching and infiltration, together cause the occurrence of large-grained Fe-sulphide minerals in the extremely thick coal seam of the Xishanyao formation in the Zhundong coalfield. Full article
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17 pages, 502 KiB  
Article
Influence of Various Cooking Methods on Selenium Concentrations in Commonly Consumed Seafood Species in Thailand
by Narisa Rueangsri, Kunchit Judprasong, Piyanut Sridonpai, Nunnapus Laitip, Jörg Feldmann and Alongkote Singhato
Foods 2025, 14(15), 2700; https://doi.org/10.3390/foods14152700 (registering DOI) - 31 Jul 2025
Viewed by 167
Abstract
Selenium (Se) is an important trace element in our body; however, food composition data remain limited due to analytical challenges and interferences. Seafood, abundant in Thailand, is recognized as a rich source of Se. This study aimed to expand knowledge on Se content [...] Read more.
Selenium (Se) is an important trace element in our body; however, food composition data remain limited due to analytical challenges and interferences. Seafood, abundant in Thailand, is recognized as a rich source of Se. This study aimed to expand knowledge on Se content in seafood prepared using traditional Thai cooking methods. Twenty seafood species were selected and prepared by boiling, frying, and grilling. Inductively Coupled Plasma–Triple Quadrupole–Mass Spectrometry (ICP-MS/MS) was used to analyze total Se contents in selected seafood species. Results revealed significant variation in Se content across species and cooking methods. The Indo-Pacific horseshoe crab showed the highest Se concentration, with fried samples reaching 193.9 μg/100 g. Se concentrations were in the range of 8.6–155.5 μg/100 g (fresh), 14.3–106.6 μg/100 g (boiled), 17.3–193.9 μg/100 g (fried), and 7.3–160.1 μg/100 g (grilled). Results found significant effects of species and cooking method on Se content (p < 0.05). Fried seafood exhibited the highest estimated marginal mean Se concentration (a 78.8 μg/100 g edible portion), significantly higher than other methods. True retention (%TR) of Se ranged from 40.4% to 100%, depending on species and method. Bigfin reef squid, wedge shell, and silver pomfret showed the highest %TR (100%), while splendid squid exhibited the lowest (52.5%). Significant interaction effects on %TR were also observed (p < 0.05). Fried seafood had the highest mean %TR (88.8%), followed by grilled (82.1%) and boiled (79.7%). These findings highlight the effects of both species and cooking method on Se retention, emphasizing the nutritional value of selected seafood in preserving bioavailable Se after cooking. Full article
(This article belongs to the Section Foods of Marine Origin)
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12 pages, 1641 KiB  
Article
Intraspecific Variations in Ecomorphological Functional Traits of Montane Stream-Dwelling Frogs Were Driven by Their Microhabitat Conditions
by Xiwen Peng, Da Kang, Guangfeng Chen, Suwen Hu, Zijian Sun and Tian Zhao
Animals 2025, 15(15), 2243; https://doi.org/10.3390/ani15152243 - 30 Jul 2025
Viewed by 200
Abstract
Understanding how habitat conditions drive morphological adaptations in animals is critical in ecology, yet amphibian studies remain limited. This study investigated intraspecific variation in ecomorphological traits of three montane stream-dwelling frogs (Quasipaa boulengeri, Amolops sinensis, and Odorrana margaratae) across [...] Read more.
Understanding how habitat conditions drive morphological adaptations in animals is critical in ecology, yet amphibian studies remain limited. This study investigated intraspecific variation in ecomorphological traits of three montane stream-dwelling frogs (Quasipaa boulengeri, Amolops sinensis, and Odorrana margaratae) across elevation gradients in Tianping Mountain, China. Using morphological measurements and environmental variables collected from ten transects, we analyzed functional traits related to feeding and locomotion and assessed their associations with microhabitat variables. Significant trait differences between low- and high-elevation groups were detected only in Q. boulengeri, with high-elevation individuals exhibiting greater body mass and shorter hindlimbs. Redundancy analysis demonstrated that microhabitat variables, particularly air humidity, flow rate, and rock coverage, were linked to trait variations. For example, air humidity and flow rate significantly influenced Q. boulengeri’s body and limb proportions, while flow rate affected A. sinensis’s snout and limb morphology. In addition, sex and seasonal effects were also associated with trait variations. These results underscore amphibians’ phenotypic plasticity in response to the environment and highlight the role of microhabitat complexity in shaping traits. By linking habitat heterogeneity to eco-morphology, this study advocates for conservation strategies that preserve varied stream environments to support amphibian resilience amid environmental changes. Full article
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16 pages, 686 KiB  
Article
Age- and Sex-Specific Reference Values for Handgrip Strength Among Healthy Tunisian Adolescents
by Souhail Bchini, Ismail Dergaa, Dhouha Moussaoui, Halil İbrahim Ceylan, Taoufik Selmi, Raul Ioan Muntean and Nadhir Hammami
Medicina 2025, 61(8), 1383; https://doi.org/10.3390/medicina61081383 - 30 Jul 2025
Viewed by 218
Abstract
Background and Objectives: Handgrip strength represents a critical indicator of physical fitness and nutritional status in adolescents, yet population-specific reference values remain limited in developing countries. Understanding age- and sex-specific variations is crucial for accurate clinical assessment and effective health monitoring. The objective [...] Read more.
Background and Objectives: Handgrip strength represents a critical indicator of physical fitness and nutritional status in adolescents, yet population-specific reference values remain limited in developing countries. Understanding age- and sex-specific variations is crucial for accurate clinical assessment and effective health monitoring. The objective of this study was to establish comprehensive reference values for handgrip strength in healthy Tunisian adolescents aged 13–19 years and examine sex and age group differences in these measures. Materials and Methods: This cross-sectional study was conducted between September 2024 and June 2025, involving a sample of 950 participants (482 males, 468 females) aged 13–19 years from northwest Tunisia. Handgrip strength was measured using standardized dynamometry protocols for both hands. Anthropometric measurements included height, weight, and body mass index. Percentile curves were generated using the LMS method, and correlations between handgrip strength and anthropometric variables were analyzed using Pearson correlation coefficients. Results: Males demonstrated significantly higher handgrip strength than females from age 13 onward (13 years: p = 0.021; 14–19 years: p ≤ 0.001). Effect sizes for sex differences were consistently large across age groups (Cohen’s d range: 0.53–2.09 for the dominant hand). Mean dominant handgrip strength ranged from 25.60 ± 7.73 kg to 47.60 ± 12.45 kg in males and 21.90 ± 6.13 kg to 28.40 ± 4.74 kg in females across age groups. After adjusting for body mass, sex differences remained significant between groups (13 years: p = 0.014; d= 1.5; 14–19 years: p ≤ 0.001; d: 1.71–3.12). Strong positive correlations emerged between handgrip strength and height (males: r = 0.748, females: r = 0.601), body mass (males: r = 0.659, females: r = 0.601), and body mass index (BMI) (males: r = 0.391, females: r = 0.461). Body mass and height emerged as the strongest predictors of handgrip strength in both sexes, while BMI showed a smaller but still significant contribution. Conclusions: This study provides the first comprehensive age- and sex-specific reference values for handgrip strength in Tunisian adolescents. Healthcare providers can utilize these percentile charts for the clinical assessment and identification of musculoskeletal fitness deficits. The results suggest its use in educational and clinical contexts. Full article
(This article belongs to the Section Sports Medicine and Sports Traumatology)
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26 pages, 11108 KiB  
Article
Warming in the Maternal Environment Alters Seed Performance and Genetic Diversity of Stylosanthes capitata, a Tropical Legume Forage
by Priscila Marlys Sá Rivas, Fernando Bonifácio-Anacleto, Ivan Schuster, Carlos Alberto Martinez and Ana Lilia Alzate-Marin
Genes 2025, 16(8), 913; https://doi.org/10.3390/genes16080913 (registering DOI) - 30 Jul 2025
Viewed by 242
Abstract
Background/Objectives: Global warming and rising CO2 concentrations pose significant challenges to plant systems. Amid these pressures, this study contributes to understanding how tropical species respond by simultaneously evaluating reproductive and genetic traits. It specifically investigates the effects of maternal exposure to [...] Read more.
Background/Objectives: Global warming and rising CO2 concentrations pose significant challenges to plant systems. Amid these pressures, this study contributes to understanding how tropical species respond by simultaneously evaluating reproductive and genetic traits. It specifically investigates the effects of maternal exposure to warming and elevated CO2 on progeny physiology, genetic diversity, and population structure in Stylosanthes capitata, a resilient forage legume native to Brazil. Methods: Maternal plants were cultivated under controlled treatments, including ambient conditions (control), elevated CO2 at 600 ppm (eCO2), elevated temperature at +2 °C (eTE), and their combined exposure (eTEeCO2), within a Trop-T-FACE field facility (Temperature Free-Air Controlled Enhancement and Free-Air Carbon Dioxide Enrichment). Seed traits (seeds per inflorescence, hundred-seed mass, abortion, non-viable seeds, coat color, germination at 32, 40, 71 weeks) and abnormal seedling rates were quantified. Genetic diversity metrics included the average (A) and effective (Ae) number of alleles, observed (Ho) and expected (He) heterozygosity, and inbreeding coefficient (Fis). Population structure was assessed using Principal Coordinates Analysis (PCoA), Analysis of Molecular Variance (AMOVA), number of migrants per generation (Nm), and genetic differentiation index (Fst). Two- and three-way Analysis of Variance (ANOVA) were used to evaluate factor effects. Results: Compared to control conditions, warming increased seeds per inflorescence (+46%), reduced abortion (−42.9%), non-viable seeds (−57%), and altered coat color. The germination speed index (GSI +23.5%) and germination rate (Gr +11%) improved with warming; combined treatments decreased germination time (GT −9.6%). Storage preserved germination traits, with warming enhancing performance over time and reducing abnormal seedlings (−54.5%). Conversely, elevated CO2 shortened GSI in late stages, impairing germination efficiency. Warming reduced Ae (−35%), He (−20%), and raised Fis (maternal 0.50, progeny 0.58), consistent with the species’ mixed mating system; A and Ho were unaffected. Allele frequency shifts suggested selective pressure under eTE. Warming induced slight structure in PCoA, and AMOVA detected 1% (maternal) and 9% (progeny) variation. Fst = 0.06 and Nm = 3.8 imply environmental influence without isolation. Conclusions: Warming significantly shapes seed quality, reproductive success, and genetic diversity in S. capitata. Improved reproduction and germination suggest adaptive advantages, but higher inbreeding and reduced diversity may constrain long-term resilience. The findings underscore the need for genetic monitoring and broader genetic bases in cultivars confronting environmental stressors. Full article
(This article belongs to the Special Issue Genetics and Breeding of Forage)
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16 pages, 2030 KiB  
Article
Study on Comb-Drive MEMS Acceleration Sensor Used for Medical Purposes: Monitoring of Balance Disorders
by Michał Szermer and Jacek Nazdrowicz
Electronics 2025, 14(15), 3033; https://doi.org/10.3390/electronics14153033 - 30 Jul 2025
Viewed by 219
Abstract
This article presents a comprehensive modeling and simulation framework for a capacitive MEMS accelerometer integrated with a sigma-delta analog-to-digital converter (ADC), with a focus on applications in wearable health and motion monitoring devices. The accelerometer used in the system is connected to a [...] Read more.
This article presents a comprehensive modeling and simulation framework for a capacitive MEMS accelerometer integrated with a sigma-delta analog-to-digital converter (ADC), with a focus on applications in wearable health and motion monitoring devices. The accelerometer used in the system is connected to a smartphone equipped with dedicated software and will be used to assess the risk of falling, which is crucial for patients with balance disorders. The authors designed the accelerometer with special attention paid to the specification required in a system, where the acceleration is ±2 g and the frequency is 100 Hz. They investigated the sensor’s behavior in the DC, AC, and time domains, capturing both the mechanical response of the proof mass and the resulting changes in output capacitance due to external acceleration. A key component of the simulation is the implementation of a second-order sigma-delta modulator designed to digitize the small capacitance variations generated by the sensor. The Simulink model includes the complete signal path from analog input to quantization, filtering, decimation, and digital-to-analog reconstruction. By combining MEMS+ modeling with MATLAB-based system-level simulations, the workflow offers a fast and flexible alternative to traditional finite element methods and facilitates early-stage design optimization for MEMS sensor systems intended for real-world deployment. Full article
(This article belongs to the Special Issue Wearable Sensors for Human Position, Attitude and Motion Tracking)
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19 pages, 10777 KiB  
Article
Improving Durability and Mechanical Properties of Silty Sand Stabilized with Geopolymer and Nanosilica Composites
by Mojtaba Jafari Kermanipour, Mohammad Hossein Bagheripour and Ehsan Yaghoubi
J. Compos. Sci. 2025, 9(8), 397; https://doi.org/10.3390/jcs9080397 - 30 Jul 2025
Viewed by 177
Abstract
This study investigates the effectiveness of geopolymer-based binders for the stabilization of silty sand, aiming to improve its strength and durability under cyclic environmental conditions. A composite binder consisting of Ground Granulated Blast-furnace Slag (GGBS) and Recycled Glass Powder (RGP), modified with nano [...] Read more.
This study investigates the effectiveness of geopolymer-based binders for the stabilization of silty sand, aiming to improve its strength and durability under cyclic environmental conditions. A composite binder consisting of Ground Granulated Blast-furnace Slag (GGBS) and Recycled Glass Powder (RGP), modified with nano poly aluminum silicate (PAS), was used to treat the soil. The long-term performance of the stabilized soil was evaluated under cyclic wetting–drying (W–D) conditions. The influence of PAS content on the mechanical strength, environmental safety, and durability of the stabilized soil was assessed through a series of laboratory tests. Key parameters, including unconfined compressive strength (UCS), mass retention, pH variation, ion leaching, and microstructural development, were analyzed using field emission scanning electron microscopy (FE-SEM) and energy-dispersive X-ray spectroscopy (EDS). Results revealed that GGBS-stabilized specimens maintained over 90% of their original strength and mass after eight W–D cycles, indicating excellent durability. In contrast, RGP-stabilized samples exhibited early strength degradation, with up to an 80% reduction in UCS and 10% mass loss. Environmental evaluations confirmed that leachate concentrations remained within acceptable toxicity limits. Microstructural analysis further highlighted the critical role of PAS in enhancing the chemical stability and long-term performance of the stabilized soil matrix. Full article
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24 pages, 11697 KiB  
Article
Layered Production Allocation Method for Dual-Gas Co-Production Wells
by Guangai Wu, Zhun Li, Yanfeng Cao, Jifei Yu, Guoqing Han and Zhisheng Xing
Energies 2025, 18(15), 4039; https://doi.org/10.3390/en18154039 - 29 Jul 2025
Viewed by 162
Abstract
The synergistic development of low-permeability reservoirs such as deep coalbed methane (CBM) and tight gas has emerged as a key technology to reduce development costs, enhance single-well productivity, and improve gas recovery. However, due to fundamental differences between coal seams and tight sandstones [...] Read more.
The synergistic development of low-permeability reservoirs such as deep coalbed methane (CBM) and tight gas has emerged as a key technology to reduce development costs, enhance single-well productivity, and improve gas recovery. However, due to fundamental differences between coal seams and tight sandstones in their pore structure, permeability, water saturation, and pressure sensitivity, significant variations exist in their flow capacities and fluid production behaviors. To address the challenges of production allocation and main reservoir identification in the co-development of CBM and tight gas within deep gas-bearing basins, this study employs the transient multiphase flow simulation software OLGA to construct a representative dual-gas co-production well model. The regulatory mechanisms of the gas–liquid distribution, deliquification efficiency, and interlayer interference under two typical vertical stacking relationships—“coal over sand” and “sand over coal”—are systematically analyzed with respect to different tubing setting depths. A high-precision dynamic production allocation method is proposed, which couples the wellbore structure with real-time monitoring parameters. The results demonstrate that positioning the tubing near the bottom of both reservoirs significantly enhances the deliquification efficiency and bottomhole pressure differential, reduces the liquid holdup in the wellbore, and improves the synergistic productivity of the dual-reservoirs, achieving optimal drainage and production performance. Building upon this, a physically constrained model integrating real-time monitoring data—such as the gas and liquid production from tubing and casing, wellhead pressures, and other parameters—is established. Specifically, the model is built upon fundamental physical constraints, including mass conservation and the pressure equilibrium, to logically model the flow paths and phase distribution behaviors of the gas–liquid two-phase flow. This enables the accurate derivation of the respective contributions of each reservoir interval and dynamic production allocation without the need for downhole logging. Validation results show that the proposed method reliably reconstructs reservoir contribution rates under various operational conditions and wellbore configurations. Through a comparison of calculated and simulated results, the maximum relative error occurs during abrupt changes in the production capacity, approximately 6.37%, while for most time periods, the error remains within 1%, with an average error of 0.49% throughout the process. These results substantially improve the timeliness and accuracy of the reservoir identification. This study offers a novel approach for the co-optimization of complex multi-reservoir gas fields, enriching the theoretical framework of dual-gas co-production and providing technically adaptive solutions and engineering guidance for multilayer unconventional gas exploitation. Full article
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33 pages, 3764 KiB  
Article
Cu2+ and Zn2+ Ions Affecting Biochemical Paths and DNA Methylation of Rye (Secale cereale L.) Anther Culture Influencing Plant Regeneration Efficiency
by Wioletta Monika Dynkowska, Renata Orłowska, Piotr Waligórski and Piotr Tomasz Bednarek
Cells 2025, 14(15), 1167; https://doi.org/10.3390/cells14151167 - 29 Jul 2025
Viewed by 124
Abstract
Rye regeneration in anther cultures is problematic and affected by albino plants. DNA methylation changes linked to Cu2+ ions in the induction medium affect reprogramming microspores from gametophytic to sporophytic path. Alternations in S-adenosyl-L-methionine (SAM), glutathione (GSH), or β-glucans and changes in [...] Read more.
Rye regeneration in anther cultures is problematic and affected by albino plants. DNA methylation changes linked to Cu2+ ions in the induction medium affect reprogramming microspores from gametophytic to sporophytic path. Alternations in S-adenosyl-L-methionine (SAM), glutathione (GSH), or β-glucans and changes in DNA methylation in regenerants obtained under different in vitro culture conditions suggest a crucial role of biochemical pathways. Thus, understanding epigenetic and biochemical changes arising from the action of Cu2+ and Zn2+ that participate in enzymatic complexes may stimulate progress in rye doubled haploid plant regeneration. The Methylation-Sensitive Amplified Fragment Length Polymorphism approach was implemented to identify markers related to DNA methylation and sequence changes following the quantification of variation types, including symmetric and asymmetric sequence contexts. Reverse-Phase High-Pressure Liquid Chromatography (RP-HPLC) connected with mass spectrometry was utilized to determine SAM, GSH, and glutathione disulfide, as well as phytohormones, and RP-HPLC with a fluorescence detector to study polyamines changes originating in rye regenerants due to Cu2+ or Zn2+ presence in the induction medium. Multivariate and regression analysis revealed that regenerants derived from two lines treated with Cu2+ and those treated with Zn2+ formed distinct groups based on DNA sequence and methylation markers. Zn2+ treated and control samples formed separate groups. Also, Cu2+ discriminated between controls and treated samples, but the separation was less apparent. Principal coordinate analysis explained 85% of the total variance based on sequence variation and 69% of the variance based on DNA methylation changes. Significant differences in DNA methylation characteristics were confirmed, with demethylation in the CG context explaining up to 89% of the variance across genotypes. Biochemical profiles also demonstrated differences between controls and treated samples. The changes had different effects on green and albino plant regeneration efficiency, with cadaverine (Cad) and SAM affecting regeneration parameters the most. Analyses of the enzymes depend on the Cu2+ or Zn2+ ions and are implemented in the synthesis of Cad, or SAM, which showed that some of them could be candidates for genome editing. Alternatively, manipulating SAM, GSH, and Cad may improve green plant regeneration efficiency in rye. Full article
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