Search Results (801)

Mango peels have great potential for upcycling in the food industry. This study addressed important knowledge gaps regarding mango peel drying, namely, the effect of drying on mango peels’ bound phenolics, and the impact of prior freezing on the composition of hot air-dried mango peels. Hence, the effect of freeze drying (FD) (0.10 mbar; −63 °C (condenser temperature); 25 °C (shelf temperature); 96 h), hot air drying (HAD) (65 °C; 48 h), and HAD preceded by freezing (FZ + HAD) (−20 °C; 30 days) on mango peels’ composition, antioxidant capacity, and technological properties was evaluated. Drying did not affect fiber content; however, it caused slight modifications in carbohydrate composition of fiber. Regarding antioxidant compounds, FD, HAD, and FZ + HAD reduced vitamin C by 9%, 53%, and 71%, respectively. FD preserved all free phenolics, while HAD and FZ + HAD decreased most of them, with reductions ranging from 20 to 42% and 17 to 71%, respectively. However, FD, HAD, and FZ + HAD reduced 9, 2, and 6 of the 10 bound phenolics identified, respectively, and decreased their antioxidant capacity. Finally, all identified carotenoids were reduced by FZ + HAD, whereas FD and HAD decreased only violaxanthin. Regarding technological properties, FD showed the highest and lowest oil and water absorption capacities. In conclusion, these findings demonstrated that prior freezing exacerbated the loss of antioxidants during HAD. Full article

Façades account for approximately 15–20% of a building’s embodied carbon, making them a key target for material decarbonization. While bio-composites are increasingly explored for façade insulation, cladding systems remain dominated by carbon-intensive materials such as aluminum and fiber-reinforced polymers (FRPs). This paper presents findings from a study investigating the use of food-waste-derived bulk fillers in bio-composite materials for façade cladding applications. Several food-waste streams, including hazelnut and pistachio shells, date seeds, avocado and mango pits, tea leaves, and brewing waste, were processed into fine powders (<0.125 μm) and combined with a furan-based biobased thermoset resin to produce flat composite sheets. The samples were evaluated through mechanical testing (flexural strength, stiffness, and impact resistance), water absorption, freeze–thaw durability, and optical microscopy to assess microstructural characteristics before and after testing. The results reveal substantial performance differences between waste streams. In particular, hazelnut and pistachio shell fillers produced bio-composites suitable for façade cladding, achieving flexural strengths of 62.6 MPa and 53.6 MPa and impact strengths of 3.42 kJ/m² and 1.39 kJ/m², respectively. These findings demonstrate the potential of food-waste-based bio-composites as low-carbon façade cladding materials and highlight future opportunities for optimization of processing, supply chains, and material design. Full article

Comprehensive characterization of the mango peel volatilome is essential to revealing its aromatic potential and enabling its revalorization as a natural flavoring. The volatile profile of Mangifera indica L. var. Osteen peels at three ripening stages (green, ripe, overripe) was analyzed before and after thermal drying (45 °C, 18 h): an unavoidable stabilization step for valorization applications. HS–SPME/GC–MS enabled the identification of 76 volatile compounds across different key aroma-contributing families: monoterpenes, sesquiterpenes, alcohols, aldehydes, ketones, esters, furanics and norisoprenoids. The ripening stage significantly influenced the qualitative and quantitative volatilome in fresh samples but drying heavily reduced those differences. Multivariate analyses confirmed that the drying process is the dominant factor shaping the stabilized peels’ volatilome. These findings underscore the industrial relevance of this side-stream: regardless of ripening stage, mango peels can be uniformly stabilized to be upcycled into aroma-rich ingredients. It simplifies raw material sourcing and supports food waste revalorization strategies in flavor and fragrance developments. Full article

Mango is the second most widely cultivated tropical fruit in the world. Its harvesting mainly relies on manual labor. During the harvest season, the hot weather leads to low working efficiency and high labor costs. Current research on automatic mango harvesting mainly focuses on locating the fruit stem harvesting point, followed by stem clamping and cutting. However, these methods are less effective when the stem is occluded. To address these issues, this study first acquires images of four mango varieties in a mixed cultivation orchard and builds a dataset. Mango detection and occlusion-state classification models are then established based on YOLOv11m and YOLOv8l-cls, respectively. The detection model achieves an AP_0.5–0.95 (average precision at IoU = 0.50:0.05:0.95) of 90.21%, and the accuracy of the classification model is 96.9%. Second, based on the mango growth characteristics, detected mango bounding boxes and binocular vision, we propose a spatial localization method for the mango grasping point. Building on this, a mango-grasping and stem-cutting end-effector is designed. Finally, a mango harvesting robot system is developed, and verification experiments are carried out. The experimental results show that the harvesting method and procedure are well-suited for situations where the fruit stem is occluded, as well as for fruits with no occlusion or partial occlusion. The mango grasping success rate reaches 96.74%, the stem cutting success rate is 91.30%, and the fruit injury rate is less than 5%. The average image processing time is 119.4 ms. The results prove the feasibility of the proposed methods. Full article

Mango seed kernels, an underutilized by-product of the mango pulping industries, are a rich supplier of metabolites, specifically phenolic and flavonoid compounds. These compounds have potential health benefits. The present study aims to optimize the solvent-assisted conditions for polyphenol extraction from mango seed kernels by using the Box–Behnken design (BBD) and response surface methodology (RSM). Moreover, the effect of the solvent-to-solid ratio (5:1 to 25:1, mL/g), extraction temperature (30–70 °C), and extraction time (60–120 min) on the polyphenol yield was investigated. The optimal conditions of a solvent-to-solid ratio of 12 (mL/g), extraction temperature of 53 °C, and extraction time of 97 min showed the maximum yield of dried extract. In optimal conditions, the extract contained a total phenolic content of 110.02 ± 0.50 mg gallic acid equivalent (GAE)/g, total flavonoids of 24.58 ± 0.09 mg quercetin equivalent (QE)/g, 64.21 ± 0.12% inhibition of DPPH, and 53.25 ± 0.23% ABTS radical scavenging. Moreover, the extract at 500 mg/mL concentration showed the highest anti-bacterial activity against pathogenic bacteria of Escherichia coli and Staphylococcus aureus. Gallic acid, mangiferin, rutin, ferulic acid, cinnamic acid, and quercetin were noted in mango seed kernel extract obtained at optimal extraction conditions. Overall, a rapid and optimal methodology is reported for extracting, identifying, and quantifying polyphenols from mango seed kernels. Full article

Post-harvest losses of ‘Cruiser’ cantaloupe reach ~15% in arid regions of Mexico, representing substantial wasted water and embedded greenhouse-gas emissions. This study presents an open-access, low-temperature maceration protocol for converting cosmetically rejected fruit into a carotenoid-rich spirit at rural scale. A 5-day maceration at 20 °C (15% pulp, 20% v/v ethanol) preserved color, β-carotene, and antioxidant capacity over 90 days of storage. Shelf-life predictions beyond this period are model-based and require long-term validation. Trained assessors characterized the beverage with a favorable aromatic profile driven by fruity esters and floral terpenes. Life-cycle results indicated lower cradle-to-gate impacts than reference mango spirits, and composting of pomace generated a mature soil amendment. A simplified techno-economic scenario suggests potential for rural processing but excludes taxation, licensing, and regulatory compliance; thus, economic feasibility cannot yet be confirmed. Overall, this study provides a proof-of-concept pathway for valorizing cantaloupe waste through low-temperature maceration and identifies critical analytical, regulatory, and economic aspects needed for future scale-up. Full article

Perfluoroalkyl and polyfluoroalkyl substances (PFASs) have emerged as contaminants of global concern due to their persistence and potential health risks. PFASs pose potential pollution risks in mango cultivation and production. This study investigated pollution characteristics and conducted a comprehensive risk assessment of PFASs in soil–mango systems within the Nanfan District of Hainan, China. The results revealed that total PFAS concentrations in soil ranged from 0.18 to 1.07 ng/g, with PFHpA and PFHxA accounting for 24.9% and 21.0%, respectively. Total PFAS concentrations in mangoes ranged from 0.0019 to 0.0201 ng/g wet weight, where PFHxA and PFHpA accounted for 44.02% and 30.28%, respectively. For all PFASs, the bioaccumulation factor (BAF) in mangoes was <1, indicating limited transfer from soil to fruits. Regarding PFAS contamination sources, long-range atmospheric transport may serve as the primary pathway for PFAS contamination in soil and mangoes. Risk assessments indicated minimal ecological and dietary exposure risks, with soil ecological risk quotients (RQs) below 0.01 and edible exposure RQs below 1. This study highlights the unique contribution of short-chain PFAS to the quality and safety of tropical agricultural products and provides critical data for the safety regulation of PFASs in soil–fruit systems. Full article

This study investigated the potential of scale-up mango leaf extract (MLE) as a treatment for diabetes, a global public health concern. MLE was prepared by boiling in water, yielding 12.07% (w/w), with a bioactive mangiferin content of 165.67 ± 10.88 μg/g in the crude powder. Mechanistically, MLE demonstrated a hypoglycemic effect by stimulating glucagon-like peptide-1 (GLP-1) secretion in NCI-H716 L-cells. This occurred through activation of the MAPK signaling pathway, evidenced by increased p-ERK1/2, p-p38, and p-c-Jun expression, and the Wnt signaling pathway, shown by increased β-catenin and decreased GSK-3β and Axin1 expression, consistent with molecular docking. In a type 2 diabetic rat model, MLE administration (40 mg/kg) significantly reduced metabolic parameters, including fasting blood glucose (FBG), body weight, cholesterol (CHOL), triglycerides (TGs), and HbA1c. Notably, MLE lowered serum insulin and the HOMA-IR index, and reduced serum dipeptidyl peptidase-IV (DPP-IV) levels, resulting in increased serum GLP-1, comparable to the drug sitagliptin. These findings suggest that MLE has great potential to lower blood glucose by inducing GLP-1 secretion via MAPKs and Wnt signaling pathways, positioning it as a promising candidate for alternative diabetes treatment or development as a dietary supplement. Full article

Recent research has focused on yogurts supplemented with plant-derived and apiculture ingredients to enhance functional properties. This study evaluates the symbiotic potential of provitamin A carotenoids, dietary fiber, and oligosaccharides from carrots, mangoes, and honeydew honey in probiotic-enriched bioyogurt. Formulations were assessed during fermentation (45 °C ± 1 °C for 5 h) and refrigerated storage (4 °C ± 1 °C for 21 days). Probiotic and starter culture viability was determined using pour-plate counts on MRS agar. Physicochemical parameters including pH, titratable acidity, total soluble solids, water-holding capacity, and antioxidant metrics (total phenolics and carotenoids) were analyzed. After 21 days of storage, the probiotic culture (VEGE 092) reached 10.26 log CFU/mL and the starter culture (YOFLEX) achieved 8.66 log CFU/mL, maintaining therapeutic thresholds. Total carotenoid content increased significantly (p < 0.05) from 2.15 to 3.96 µg β-carotene/g, indicating synergistic interactions between lactic acid bacteria and plant-derived bioactive compounds. These findings demonstrate that combining plant-derived carotenoids, prebiotic fibers, and honeydew oligosaccharides effectively maintains probiotic viability and enhances antioxidant stability throughout fermentation and refrigerated storage, supporting the development of functional dairy products with improved nutritional profiles. Full article

Anthocyanins are important secondary metabolites that impart color to fruits, and their biosynthesis is regulated by light. AP2/ERF transcription factors represent one of the largest TF families in plants and play pivotal roles in regulating plant growth and development, secondary metabolism, and stress responses. However, their comprehensive profile in mango (Mangifera indica L.) and their role in mango anthocyanin biosynthesis remain largely unclear. In this study, genome-wide identification and analysis of the AP2/ERF gene family in mango were conducted. A total of 240 family members were identified and classified into five subfamilies. Phylogenetic tree, conserved motif, and gene structure analyses revealed high conservation within the same subfamily and significant divergence among different subfamilies. Synteny analysis indicated that segmental and tandem duplication events played a major role in the expansion of the MiAP2/ERF family. Organ-specific expression profiles based on RNA-seq data uncovered the expression patterns of MiAP2/ERF genes in different plant organs. Furthermore, RNA-seq analyses related to light-induced anthocyanin accumulation, including preharvest “bagging–debagging” treatment and postharvest UV-B/white light and blue light treatments, identified a subset of MiAP2/ERF genes with significant light-responsive trends. The expression patterns of six blue-light-induced MiAP2/ERF genes were validated by means of qPCR. In summary, this study provides a comprehensive theoretical characterization of the AP2/ERF family in mango and reveals its potential role in light-induced anthocyanin accumulation, thereby establishing a solid theoretical foundation for subsequent investigations into gene functions and molecular mechanisms. Full article

Accurate geospatial inventories of fruit orchards are essential for precision horticulture and food security, yet Pakistan lacks consistent spatial datasets at district and tehsil levels. This study presents the first national-scale, object-based Random Forest (RF) framework for orchard delineation and yield estimation by integrating multi-temporal Sentinel-2 imagery on Google Earth Engine (GEE) with high-resolution Pakistan Remote Sensing Satellite-1 (PRSS-1) data. Among the tested classifiers, RF achieved the highest performance on Sentinel-2 data (Overall Accuracy (OA) = 79.0%, kappa (κ) = 0.78), outperforming Support Vector Machines (OA = 74.5%, κ = 0.74) and Gradient Boosting Decision Trees (OA = 73.8%, κ = 0.73), with statistical significance confirmed (McNemar’s χ², p < 0.01). Integrating RF with Object-Based Image Analysis (OBIA) on PRSS-1 imagery further enhanced boundary precision (OA = 92.6%, κ = 0.89), increasing Producer’s and User’s accuracies to 90.4% and 91.5%, and increasing Intersection-over-Union (IoU) from 0.71 to 0.86 (p < 0.01). Regression-based yield modeling using field-observed data revealed that mean- and median vegetation index aggregations provided the most stable predictions (R² = 0.77–0.79; RMSE = 72–105 kg tree⁻¹), while extreme-value models showed higher errors (R² = 0.46–0.56; RMSE > 560 kg tree⁻¹). The resulting multisensory geospatial inventory of citrus and mango orchards establishes a scalable, transferable, and operationally viable framework for orchard mapping yield forecasting, and resource planning, demonstrating the strategic value of national satellite assets for food security monitoring in data-scarce regions. Full article

Background/Objectives: Insufficient dietary fiber intake contributes to gut microbiota dysbiosis, systemic inflammation, and the onset of obesity-related metabolic disorders. Agro-industrial by-products have emerged as sustainable sources to restore microbial and metabolic balance. This study aimed to evaluate the effects of a mango bagasse- and peel-based confectionery (MC) on gut microbiota composition, short-chain fatty acids (SCFAs), and hepatic gene expression in Wistar rats fed either a standard diet (STD) or a high-fat diet (HFD). Methods: Twenty-four rats were randomly assigned to four groups (STD, MC-STD, HFD, MC-HFD) and treated for 11 weeks. Eating behavior, body composition, microbiota composition, SCFAs, and hepatic transcriptomics were evaluated. Results: MC supplementation did not significantly alter weight gain or SCFA levels but shifted clustering patterns in principal component analysis, indicating a distinct dietary response. Microbiota analysis revealed a trend toward lower relative abundances of obesogenic species such as Phascolarctobacterium faecium and Ruminococcus torques, while Intestimonas butyriciproducens and Anaerostipes hadrus were modulated according to diet type. Transcriptomic profiling demonstrated consistent downregulation of lipid metabolism–related genes (Cyp4a14, Hmgcs1, Cyp51, Fads1), linked to PPAR signaling pathways. Conclusions: MC supplementation may beneficially modulate the gut–liver axis and highlights the nutritional potential of fruit by-products as functional ingredients to promote metabolic health under high-fat dietary conditions. Full article

Biological control using beneficial microbes offers a sustainable alternative to chemical fungicides for managing postharvest diseases. This study reports the isolation and characterization of Bacillus amyloliquefaciens AsL-1 from the latex of Alstonia scholaris (L.) R. Br., unconventional ecological niche. The cell-free supernatant (CFS) of AsL-1 showed strong antifungal activity, inhibiting the growth of Colletotrichum musae (48.89 ± 0.57%), Glomerella cingulata (52.22 ± 0.00%), Fusarium graminearum (47.78 ± 0.57%), and Colletotrichum gloeosporioides (47.78 ± 0.00%) in vitro. Microscopy revealed that the CFS disrupted fungal development by blocking conidial germination and appressorium formation, and in C. gloeosporioides caused melanization defects linked to reduced virulence. In vivo tests on mango fruit confirmed that AsL-1 significantly decreased anthracnose lesion size and disease incidence. Protein analyses (SDS-PAGE, gel overlay, and LC-MS/MS) identified two antifungal proteins (24 and 16 kDa), corresponding to β-1,3-1,4-glucanase and flagellin. The detected β-1,3-1,4-glucanase activity indicates its role in degrading fungal cell walls and interfering with melanin biosynthesis pathways essential for pathogenicity. Overall, these findings highlight B. amyloliquefaciens AsL-1 as a promising protein-based biocontrol agent and show that latex-associated microbes may serve as valuable sources of new antifungal strategies. Full article

The mango (Mangifera indica L.) is a commonly cultivated tropical fruit across the globe. It is known to be rich in carotenoids, polyphenols, and vitamins, compounds that largely account for its nutritional and medicinal properties. Although the beneficial effects of mango phytochemicals have been widely documented, virtually no studies have investigated extracellular vesicles (EVs) originating from mango fruit. In the presented work, we developed a workflow combining differential centrifugation, filtration, and size-exclusion chromatography for the isolation of EVs from mango pulp. The isolates were characterized in accordance with the guidelines of the International Society of Extracellular Vesicles recommendations. The optimized size-exclusion chromatography column, packed with Sepharose CL-6B beads, enabled the recovery of a high-quality EV fraction, which was characterized in terms of physicochemical properties. Additionally, proteomic analysis identified 1084 proteins, many of which are associated with antioxidant, antimicrobial, and anti-inflammatory functions. These findings provide the first comprehensive characterization of mango-derived EVs and suggest that they may contribute to the biological activity traditionally attributed to mango consumption. Full article

Mango (Mangifera indica L.) is a tropical fruit tree characterized by vigorous growth and high fruit production, making it one of Peru’s main export crops. However, its extensive vegetative development requires substantial space, limiting productivity per unit area. This study evaluated the effects of rootstock and interstock combinations on agronomic traits and fruit biometrics, highlighting the potential of interstocks to modulate tree vigor in mango orchards of Peru’s dry forest region. A total of 216 trees were established using ‘Chulucanas’ and ‘Chato’ as rootstocks and ‘Chulucanas,’ ‘Chato,’ ‘Irwin,’ and ‘Julie’ as interstocks, apically grafted with the ‘Kent’ cultivar, with a spacing of 6.0 m × 6.0 m. Tree performance was assessed after 10 years during the 2017–2019 growing seasons in Piura, Peru, under a randomized complete block design (2 × 4 factorial). The combination of the ‘Chulucanas’ rootstock with ‘Chulucanas’ and ‘Julie’ interstocks reduced tree height by 10.94% and 11.70%, respectively, facilitating orchard management and potentially increasing planting density. Yield varied significantly among growing seasons, with a 15% reduction in 2017 attributed to El Niño–Southern Oscillation (ENSO)-related increases in temperature and rainfall that affected flowering and fruit set. These results underscore the importance of cultivar selection and climate-adaptive strategies to sustain mango productivity in regions prone to climatic variability. Full article