Search Results (153)

Porcine circoviruses are significant pathogens that affect swine populations worldwide, with implications for animal health and productivity. While PCV2 is well-documented, particularly due to widespread vaccination programs, PCV3 is less understood, and its epidemiological impact is still under investigation. This study screened for PCV2 and PCV3 in pigs and wild boars across Portugal to assess their prevalence. Also, nucleotide sequence determination was performed to evaluate the genetic diversity of these viruses. Stool samples from 160 pigs belonging to different groups (quarantine, nursery, fattening and adult pigs), as well as organ samples from 120 hunted wild boars, were analyzed. Samples were collected from twelve of the eighteen mainland Portuguese districts with positive cases being detected in nine of them. Pigs had a lower prevalence of PCV2 (1.9%) than PCV3 (11.2%), but the opposite was true in wild boars (76.7% for PCV2 and 55.0% for PCV3). The lower PCV2 prevalence in pigs can be attributed to the PCV2 vaccination program implemented. Additionally, these viruses were significantly more prevalent in wild boars (90.8% were infected with at least one of the viruses) than in domestic pigs (only 12.5%). This significant difference highlights the impact of the controlled environment in pig farms on disease prevention in contrast to the higher exposure risks faced by wild boars in their natural habitat. Compared to a previous study from 2023, we observed a slight decrease in the percentage of positive cases for both PCV2 and PCV3. Phylogenetic analysis of sequences obtained by Sanger sequencing allowed us to conclude that the samples from domestic pigs belong to the PCV2a and PCV3c clades, in contrast to the PCV2-positive cases detected in domestic pigs in 2023 that were classified in the PCV2d genotype. Conversely, samples from wild boars belong to the PCV2d and PCV3a clades. These results reveal genotype differences between wild and domestic pigs and shifts from 2023 to 2024. Our findings provide some information about the circulation of these viruses and emphasize the importance of vaccination and continued monitoring for a deeper understanding of their epidemiology to mitigate potential risks to swine health and production. Full article

Background/Objectives: Population aging and the rise in chronic diseases challenge healthcare systems to adopt person-centered approaches, especially in palliative care (PC), where symptom management remains limited. Physiotherapy plays a key role in alleviating discomfort but faces inconsistent integration in Portugal due to lack of recognition. Variations in intervention methods hinder uniform care delivery, limiting timely patient access to comfort-focused treatments and knowledge. This study aims to deepen the understanding of physiotherapy’s role in Portuguese PC to improve its integration into teams and enhance patient access to comfort care. Methods: This study used a descriptive qualitative approach with online focus groups (FG), guided by Krueger and Casey’s methodology and adhering to the COREQ checklist. A non-probabilistic convenience sample of physiotherapists working in palliative care across mainland Portugal and the islands was selected based on inclusion criteria. Three FGs were planned with up to ten participants each. However, due to availability and attendance issues, only 15 physiotherapists participated: 5 in FG1 (in-hospital PC units), 6 in FG2 (inpatient units), and 4 in FG3, the minimum appropriate number from community-based units. Results: Physiotherapy plays a crucial yet underrecognized role in PC, emphasizing the need for its full integration into care teams rather than reliance on late, on-call referrals. Techniques such as positioning, mobilization, pain and dyspnea relief, adapted exercises, massage, music therapy, and emotional support are employed. Conventional physiotherapy tools are used and personalized according to the patient’s context, duration, setting, dosage, and individual needs. Conclusions: Physiotherapy should be recognized as a fundamental part of PC, contributing not only to the prolongation of life but also to ensuring comfort and dignity for patients and their families. To achieve this, its role within multidisciplinary teams must be strengthened and supported by regulations that guarantee access and the formal integration of physiotherapists. However, a significant gap remains in patients’ regular access to comfort-focused interventions at the appropriate time, perhaps due to the considerable variation in physiotherapy practices depending on the patient and care setting, which presents a challenge for knowledge development both in Portugal and globally. Full article

Background: The nursing practice environment significantly influences nurses’ job satisfaction, turnover, and burnout; therefore, it is essential to promote favorable environments to ensure the retention of qualified professionals. Improving the nursing practice environment is a low-cost organizational strategy associated with satisfaction, retaining professionals, and reducing burnout. The aim of this study was to assess the relationship between the nursing practice environment and job satisfaction, turnover, and burnout among primary healthcare nurses in Portugal. Methods: A descriptive, cross-sectional, and correlational study was carried out based on data from the RN4CAST Portugal Project. The Nurse Survey Instrument (Core Nurse Survey) of the RN4Cast Project (2018) was used for data collection. The sample consisted of 1059 nurses from fifty-five health center groups in mainland Portugal, fifteen health centers in the Autonomous Region of Madeira, and six health centers in the Autonomous Region of the Azores. Results: Primary healthcare nurses in Portugal rated the nursing practice environment as unfavorable or mixed, with an average ($\overline{\mathrm{x}}$) of 2.5 (standard deviation (SD) = 0.4), which is associated with lower job satisfaction, with an average of 2.0 (SD = 0.4), moderate intention to leave, with 40.3%, and low levels of burnout, with an average of 1.6 (SD = 0.8). There was also a negative correlation between the nursing practice environment and burnout (r = −0.28) and its dimensions. Emotional exhaustion (r = −0.35) represents the individual dimension of stress and physical exhaustion, corresponding to feelings regarding the depletion of emotional and physical resources, depersonalization (r = −0.18) represents the interpersonal context dimension of burnout, and a lack of personal accomplishment (r = −0.15) represents the self-assessment dimension of burnout and refers to feelings of incompetence and a lack of confidence and self-efficacy at work. Conclusions: The quality of the work environment is associated with greater job satisfaction and a reduction in burnout. For this reason, improving the work environment has therefore been associated with increased job satisfaction and reduced burnout among primary healthcare nurses, promoting nurse retention and the well-being of healthcare teams. Full article

Historical earthquake records are crucial for analyzing high-intensity earthquakes that occur over long periods. Since good instrumental data only date back to 1980, there are gaps in our knowledge, and qualitative assessments remain essential to expand our knowledge and integrate more information into the number of variables under analysis. This study examined the hydrological and hydrogeological effects of the 1755 Lisbon earthquake, focusing on regions near rivers and proposing new insights for intensity scales. This information is relevant for seismic risk management and mitigation, to be discussed in regional and national territorial planning strategies. Mapping revealed that most phenomena occurred along the Porto–Tomar tectonic fault, with some extending to other probable faults or geological contrasts. A comparative chart between existing intensity scales and the proposed descriptors highlights agreements and discrepancies, emphasizing the need for more detailed descriptors for intensity levels below X for river-related phenomena. The proposed descriptors include a flow increase with course alterations (intensities VI–VIII), flow suppression and eventual reset (intensity VIII or higher), abnormal current agitation and vertical wave movements (intensities VI–VIII), and cloudy (turbid) water (intensities V–VIII). This work also highlights the need to cross-reference data and the complexity of establishing correlations between effects, ancient descriptions, and descriptors for these intensity scales. Full article

The Portuguese forest plays a crucial role in maintaining ecological balance and fostering socio-economic sustainability within rural areas. Nonetheless, it is currently facing significant challenges due to the increasing intensity and frequency of forest fires observed in recent decades. The deterioration of traditional agricultural practices, the proliferation of monocultures, and alterations in land use patterns have significantly exacerbated these challenges. Consequently, the landscape has undergone considerable transformations, resulting in a decline in biodiversity and a weakening of local economies. This study examines land use in mainland Portugal from 1995 to 2018, utilising data on land occupation, land cover, and burnt areas from the Institute for Nature Conservation and Forests. The cartographic analysis of three periods—1995, 2007, and 2018—along with the fire data recorded between 1996 and 2018, enabled the observation of changes in the predominant land use and land cover (LULC) classes, particularly among forests, scrubland, and agricultural areas. The results highlight a significant increase in forested areas, especially eucalyptus, as well as urbanisation, while scrubland and agricultural areas have decreased. Using specific LULC level 4, and burnt (BA) and unburnt (NB) areas, temporary crops decreased substantially (−14% NB/−4% BA 1995–2007; −23% NB by 2018). Eucalyptus showed strong continuous growth (16% NB/35% BA 1995–2007; 23% NB/47% BA 2007–2018). Maritime pine suffered severe losses, especially in burnt areas (−42%/−28%). Cork oak remained stable (1–4% growth). Other oaks showed minimal changes. Bushes (scrubland) declined sharply post-2007 (−31% BA/−6% NB). The most significant transformation occurred between 1995 and 2007, particularly in the south of Portugal, where wildfires promoted the replacement of maritime pine with eucalyptus, a species that offers greater profitability, leading to agricultural abandonment in burned areas. Full article

Background/Objectives: Poultry meat is a popular and nutritious food, valued for its high protein content and healthy fat profile. However, like other animal products, it can contain pharmaceutical residues, including coccidiostats, antimicrobials commonly used to prevent parasitic infections caused by Eimeria species. While most monitoring focuses on raw meat, it is important to understand how these compounds behave during cooking to assess potential health risks better and ensure food safety. Methods: This study examined how five different cooking methods (roasting, grilling, and microwaving, beer and wine marinating) affect the levels of eight coccidiostat residues in 45 samples of poultry muscle collected from a supermarket located in the center of mainland Portugal from May to July 2024. After applying different cooking procedures, ionophore and synthetic coccidiostat residue levels were measured using solid–liquid extraction followed by ultrahigh-performance liquid chromatography with tandem mass spectrometry (UHPLC-MS/MS). Results are expressed as percentages of the original concentrations: 100% indicates stability, values above 100% suggest a relative increase (often due to moisture loss), and values below 100% reflect a decrease, likely from heat degradation. Results: Roasting, grilling, and microwaving all increased residue concentrations—up to 198.5%, 180.1%, and 158.4%, respectively. In contrast, marinating meat in wine or beer before cooking reduced residues to 73.1% and 72.0%, suggesting a mitigating effect. The initial concentration also influenced the outcome: samples fortified at the maximum residue limit (MRL) had an overall higher mean concentration after cooking (148.3%,) than those fortified at twice the MRL (2 MRL), which averaged 124.5%. Conclusions: These results show that cooking can significantly alter coccidiostat residue levels depending on the cooking procedures and initial concentration. Ongoing monitoring and further research are essential to better understand how cooking affects these residues and their by-products. This knowledge is key to improving food safety practices and refining consumer health risk assessments. Full article

The present contribution addresses the Heat Waves (HWs) which occurred in Portugal’s mainland during the first 24 years of the XXI century: the number of HWs, their frequency, duration and geographic localization, among other impacts, are described. In a complementary perspective, due to the significant impacts of the 2003 HW, specifically in terms of mortality, a more detailed analysis of this event is performed. For the present analysis, HWs were identified using a modified version of the Heat Wave Duration Index (HWDI) proposed by the World Meteorological Organization (WMO). During the XXI century, between 2001 and 2024, 95 HWs occurred in the Portuguese mainland. In 2024, eight HWs occurred, followed by 2009, 2015 and 2017, with seven HWs each; in terms of monthly distribution, August (17) and May (16) displayed the highest values. HWs are now included in the World Health Organization agenda of natural hazards, enhancing the importance of these events. It is time to start considering HWs and their significant impacts as an important issue, especially in countries with older populations, like Portugal. Full article

A LoRaWAN application architecture comprises three functional components: (i) nodes, which convert and wirelessly transmit data as LoRaWAN messages; (ii) gateways, which receive and forward these transmissions; and (iii) network servers, which process the received data for application delivery. The nodes convert data into LoRaWAN messages and transmit them wirelessly with the hope that one or more LoRaWAN gateway will receive the messages successfully. Then, the gateways pass on the received messages to a distant network server, where various processing steps occur before the messages are forwarded to the end application. If none of the gateways can receive the messages, then they will be lost. Although this default behaviour is suitable for some applications, there are others where ensuring messages are successfully delivered at a higher rate would be helpful. One such scenario is the application in this paper: monitoring maritime vessels and fishing equipment in offshore environments characterised by intermittent or absent shore connectivity. To address this challenge, the Custodian project was initiated to develop a maritime monitoring solution with enhanced connectivity capabilities. Two additional features are especially welcome in this scenario. The most important feature is the transmission of messages created in offshore areas to end users who are offshore, regardless of the unavailability of the ground network server. An example would be fishermen who are offshore and wish to position their fishing equipment, also offshore, based on location data transmitted from nodes via LoRaWAN, even when both entities are far away from the mainland. The second aspect concerns the potential use of gateway-to-gateway communications, through gateways on various ships, to transmit messages to the coast. This setup enables fishing gear and fishing vessels to be monitored from the coast, even in the absence of a direct connection. The functional constraints of conventional commercial gateways necessitated the conceptualisation and implementation of C-Mesh, a novel relay architecture that extends LoRaWAN functionality beyond standard protocol implementations. The C-Mesh integrates with the Custodian ecosystem, alongside C-Beacon and C-Point devices, while maintaining transparent compatibility with standard LoRaWAN infrastructure components through protocol-compliant gateway emulation. Thus, compatibility with both commercially available nodes and gateways and those already in deployment is guaranteed. We provide a comprehensive description of C-Mesh, describing its hardware architecture (communications, power, and self-monitoring abilities) and data processing ability (filtering duplicate messages, security, and encryption). Sea trials carried out on board a commercial fishing vessel in Sesimbra, Portugal, proved C-Mesh to be effective. Location messages derived from fishing gear left at sea were received by an end user aboard the fishing vessel, independently of the network server on land. Additionally, field tests demonstrated that a single C-Mesh deployment functioning as a signal repeater on a vessel with an antenna elevation of $15m$ above sea level achieved a quantifiable coverage extension of 13 km (representing a 20% increase in effective transmission range), demonstrating the capacity of C-Mesh to increase LoRaWAN’s coverage. Full article

Deep convective clouds, such as towering cumulus and Cumulonimbus, can endanger lives and property, also being a major hazard to aviation. This study presents the convective index (IndexCON) used operationally at the Portuguese Meteorological Watch Office. Moreover, IndexCON is evaluated against lightning and precipitation data for two years, between January 2022 and December 2023, over mainland Portugal and its surrounding areas. This index combines several European Center for Medium-Range Weather Forecasts (ECMWF) prognostic variables, such as stability indices, cloud water content, relative humidity and vertical velocity, using a fuzzy-logic approach. IndexCON performs well in the warm season (May–October), with a probability of detection (POD) of 70%, a false alarm ratio (FAR) of 30% and a probability of false detection (POFD) less than 5%, leading to a Critical Success Index (CSI) above 0.55. However, IndexCON performs worse in the cold season (November–April), when dynamical drivers are more relevant, mainly due to overestimating the convective activity, resulting in CSI and Heidke Skill Score (HSS) values below 0.3. Optimizing the membership functions partially reduces this overestimation. Finally, the added value of IndexCON was illustrated in detail for a thunderstorm episode, using satellite products, lightning and precipitation data. Full article

This study examines exceptional maximum temperature (T_max) events in mainland Portugal (1980–2024) using ERA5-Land reanalysis data at 1012 locations. To assess changes in the occurrence and temperature excess of exceptional events across two 22-year subperiods (or phases), percentile-based thresholds were adopted. An inventive severity heatmap is used to illustrate exceptional T_max changes between the two phases, which constitutes an addition to climate change research. Locations are categorised in the heatmap according to whether they experienced (i) more occurrences and more temperature excess, (ii) more occurrences but less temperature excess, (iii) fewer occurrences but more temperature excess, or (iv) fewer occurrences and less temperature excess. From the historical (1980–2002) to the contemporary (2002–2024) phase, results indicate a significant increase in the severity of extreme heat, particularly in central and southern Portugal, with over 90% of locations exhibiting a rise in exceptional event occurrences. While the Student’s t-test indicated significant differences in both occurrence and temperature excess between the phases, Sen’s slope estimation showed steady upward trends. The results point to crucial regions in the interior and southern Portugal that have warmed the most, posing growing threats to agriculture, human health, and water resources. Although slight cooling trends were observed in a few northern and central coastal regions, the overall pattern highlights an increase in extreme heat. This research is particularly relevant given the recent changes in exceptional T_max identified in Portugal, aligning with broader climate change patterns and trends. Full article

The commitment to technical and scientific innovation for appropriate interventions in buildings with wattle and daub walls requires a comprehensive and authentic approach to pre-existing structures. This involves an in-depth study of traditional heritage, aiming for durable, sustainable, and economically viable repair solutions. Most studies on wattle and daub constructions have focused on northern Portugal, given the prevalence of this building technique in the region. However, there is a knowledge gap regarding the presence and specific characteristics of these structures in the central and southern regions of mainland Portugal, as well as in the Autonomous Regions of the Azores and Madeira. This research broadens the geographical scope, encompassing the analysis of 30 buildings, providing complementary data to the existing literature and contributing to a broader understanding of the structural and functional behaviour of wattle and daub walls. The information obtained about traditional materials and construction methods is essential to guide interventions that respect the historical and cultural character of the buildings, while also promoting sustainability and economic feasibility. The results confirmed the presence of wattle and daub constructions in the various areas investigated, highlighting significant diversity in the materials used, both in the timber structures and in the infill and rendering mortars. This variability was observed not only between regions with similar characteristics but also in buildings within the same locality, reflecting local adaptations and the richness of traditional craftsmanship. These findings provide a solid foundation for future interventions and encourage the appreciation of the cultural heritage associated with this construction technique. Full article

Rural fires are becoming increasingly impactful, not only due to their rapid and intense spread but also because of the growing number of people affected. In Portugal, numerous remote housing clusters are difficult to access and are primarily occupied by seniors or individuals with disabilities. These areas are situated in high-risk wildfire zones. Therefore, it is crucial to document the locations of the individuals who are most at risk to ensure that relief efforts can be focused on and directed towards them in case of emergency. The aim of this paper is to propose an inventory plan for communities at risk of wildfire, requiring the involvement of parish councils. The plan involves the use of simple and cost-free georeferencing tools (such as Mymaps of Google) to enable these organizations to assist in municipal emergency planning and facilitate the exchange of real-time information. The outcomes of this paper will be demonstrated in three parishes in mainland Portugal, where the key benefits and limitations were identified. The goal is to enhance the project for potential replication in other areas. Full article

Bats and their ectoparasites play a crucial role in understanding the ecology and transmission of vector-borne pathogens, yet these dynamics remain poorly studied in Portugal. This study aimed to investigate the molecular occurrence of vector-borne bacteria (Anaplasma spp., Bartonella spp., Ehrlichia spp., and Rickettsia spp.) and protozoa (Babesia spp. and Theileria spp.) in ectoparasites of cave-dwelling bats. Bats were sampled from two caves in Portugal, and their ectoparasites included wing mites (Spinturnix myoti), ticks (Ixodes simplex), and bat flies (Penicillidia conspicua and Nycteribia schmidlii). Molecular analyses revealed the presence of Bartonella spp. in S. myoti and N. schmidlii. Phylogenetic inference based on the gltA gene positioned the detected genotypes close to those previously reported in bats and Nycteribiidae flies in Europe, Asia, and Africa. Notably, no DNA from Anaplasmataceae, Rickettsia spp., or piroplasmids was detected. The prevalence of S. myoti was high, with all examined bats being infested, showing notable differences in ectoparasite diversity concerning sex and cave-specific location. These findings suggest that host behavior, environmental conditions, and ectoparasite lifecycles play critical roles in shaping pathogen transmission dynamics. This study advances the understanding of bat ectoparasite–pathogen interactions in a region with limited data and highlights the need for continued research to assess the zoonotic potential and ecological impacts of the Bartonella genotypes detected herein. Full article

With the rising frequency and severity of wildfires that cause significant threats to ecosystems, public health and livelihoods, it is essential to have tools for evaluating and monitoring their impacts and the effectiveness of policy initiatives. This paper presents the development and implementation of a new calculation pipeline integrated with a web-based platform designed to provide georeferenced data on the burn severity of wildfires in mainland Portugal. The platform integrates a modular architecture that comprises a module in R and Google Earth Engine to compute standardized satellite-derived datasets on observed/historical severity for burned areas, integrated with a web portal module to facilitate the access, search, visualization, and downloading of the generated data. The platform provides open-access, multisource data from satellite missions, including MODIS, Landsat-5, -7, and -8, and Sentinel-2. It offers multitemporal burn severity products, covering up to 12 months post-fire, and incorporates three severity indicators, the delta NBR, relative difference NBR, and relativized burn ratio, derived from Normalized Burn Ratio (NBR) quarterly median composites. The platform’s modular and scalable framework also allows the integration of more spectral indices, burn severity indicators, and other wildfire perimeter databases. These design features also enable the platform to adapt to other contexts or regions beyond its current scope and regularly update burn severity products. Results from exploratory data analyses revealed the ability of satellite-based severity products to diagnose trends, assess interannual variability, and enable regional comparisons of burn severity, providing a basis for further research. In the face of climate change and societal challenges, the platform aims to support decision-making processes by providing authorities with standardized and updated information while promoting public awareness of wildfire challenges and, ultimately, contributing to the sustainability of rural landscapes. Full article

The current article seeks to present and discuss the research results as part of the ‘Magenta’ Project, which focuses on mapping whether and how nationality has been an impact factor in the context of domestic work in Portugal during the COVID-19 pandemic. This quantitative study sought to compile and organize statistical data showing the effects of COVID-19 on domestic workers in the mainland and island regions of Portugal throughout 2021. Due to the extent of the research, this article focuses only on analyzing the results that show statistically significant associations between the pandemic and the variables of different nationalities in domestic work in Portugal. Full article