Search Results (131)

Understanding the soil–plant transfer of both essential and non-essential elements is crucial for evaluating the crop nutritional quality, environmental interactions, and food safety. This study delivered a multiyear and multielement assessment under field conditions of the element uptake, translocation, and accumulation in raspberry (Rubus idaeus L.), based on data collected over two growing seasons (2024–2025) in two contrasting Romanian agroecosystems. Two commercial cultivars (Opal and Delniwa) were investigated under fertilized and unfertilized conditions. The concentrations of essential macroelements such as Ca, Mg, Na, and K, as well as trace elements (Li and Sr), were determined in soils and fruits using ICP-OES and AAS. The soil–fruit transfer was quantified through the transfer factor, assisted by a robust statistical framework which integrated spatial–temporal variability and non-parametric analysis. The results highlighted two contrasting accumulation regimes. The essential macroelements revealed a dynamic uptake pattern driven by the physiological demand, soil availability, and fertilization. K exhibited the highest transfer capacity, while Ca had a restricted translocation to the fruits, due to the intrinsic transport limitations. On the other hand, Li and Sr revealed a constrained accumulation, characterized by low concentrations, weak responsiveness to fertilization, and a strong dependence on the soil geochemical background and interannual dilution processes. The spatial variability between the cultivation sites and year-to-year changes in the dilution intensity was evidenced as the dominant driver of the transfer efficiency, while the varietal differences had a secondary but detectable role, mainly for the Ca–Sr discrimination. Overall, the results evidenced that the multielement accumulation in the raspberries was governed by the interplay between the soil geochemistry, physiological transport constraints, and environmental variability. Furthermore, the research provided a field-based, multiyear evidence supporting improved soil management, cultivar selection, as well as the strategies that may increase the fruit nutritional quality while minimizing the trace element risks. Full article

Yucca decipiens is a native species from arid and semi-arid regions with emerging nutritional and biotechnological potential. This study evaluated its proximate composition, elemental profile determined by inductively coupled plasma mass spectrometry (ICP-MS), and growth performance under nursery conditions. Proximate analysis revealed a high dietary fiber content in leaves (58.93%) and higher carbohydrate levels in stems (28.83%). Free amino acid content was significantly higher in stems (2.75 g histidine equivalents kg⁻¹) than in leaves (1.76 g kg⁻¹). Multi-elemental profiling (63 elements) showed organ-specific accumulation patterns, with essential macro- and micronutrients predominantly concentrated in leaves, including potassium (28,334 ppm) and calcium (15,345 ppm), while iron was the most abundant trace element in stems (1253 ppm). Principal component analysis (PCA) revealed clear organ-specific mineral partitioning between leaves and stems, indicating differentiated physiological roles and potential selective biomass utilization. Growth assessment conducted over a two-year period demonstrated steady biomass accumulation and good adaptive performance under nursery conditions. Overall, the results highlight the emerging nutritional and agroindustrial relevance of Yucca decipiens for applications in semi-arid environments. Full article

Background/Objectives: Endometrial cancer (EC) is a multifactorial disease influenced by metabolic, hormonal, and environmental factors. Trace and macroelements play a critical role in cellular homeostasis, oxidative stress, and tumor progression; however, their relationship with EC grading and clinical characteristics remains insufficiently understood. Methods: This study evaluated the concentrations of selected macro- and trace elements (Na, K, Ca, P, Mg, Mn, Cu, Zn, Be, As, Cr, Mo, Ti, Tl, Pb) in patients with endometrial cancer (G1–G3) and a control group (C). Elemental analysis was performed using inductively coupled plasma optical emission spectrometry (ICP-OES). Associations between elemental concentrations and clinicopathological variables, including age, body mass index (BMI), menopausal status, diabetes, and smoking, were assessed using appropriate statistical tests, including ANOVA with Tukey’s post hoc analysis and Student’s t-test. Multivariate regression analysis was performed to identify independent predictors of elemental alterations. Results: Significant differences in elemental concentrations were observed across EC grading. Higher-grade tumors were associated with increased levels of Ca, P, Mg, and Mn, while Na and K showed a decreasing trend with tumor progression. No statistically significant differences were observed for Zn, Ti, Tl, or Pb across histological grades. Stratified analyses demonstrated that clinical and metabolic factors had a limited and selective impact on elemental profiles. Age and BMI were associated with minor variations in selected elements, whereas menopausal status, diabetes, and smoking showed predominantly non-significant or inconsistent effects. Multivariate analysis identified histological grade as the primary determinant of elemental alterations, while other variables exhibited weaker or element-specific associations. Conclusions: Elemental homeostasis in endometrial cancer is primarily associated with tumor progression rather than systemic metabolic or lifestyle factors. Changes in Ca-, P-, Mg-, and Mn-related pathways may reflect tumor-driven metabolic reprogramming, whereas most trace elements remain relatively stable. These findings suggest that elemental profiling may provide insight into EC biology, although its clinical utility requires further investigation. Full article

The elemental composition of the vitreous humor may reflect physiological and pathological processes occurring in the eye. The objective of this study was to provide a complex multielemental analysis of human vitreous humor. Vitreous humor samples (n = 57) were collected post-mortem during autopsies. Inductively coupled plasma mass spectrometry (ICP-MS) was employed to quantify micro-, trace-, ultra-trace, and toxic elements. The study showed the occurrence of elements at the ppm (Na, K, P, Ca, Mg), ppb (Al, Rb, Zn, Fe, Sr, Cu), and ppt (Ce, La, Nd, Tb) levels. Hierarchical clustering using Ward’s method and k-means analysis revealed four distinct clusters, including two major clusters representing the baseline macro- and microelement profile characteristic for the studied population. Correlations between elements revealed statistically significant (p < 0.05) positive and negative correlations between elements with (I) chemical similarity Ce-La, Cs-Rb, Rb-K, Ca-P, Zn-Cu, and Cs-K; (II) a possible common environmental origin, Cd-P, and Rb-P; (III) involvement in similar biological processes as K-P; and (iv) a common geochemical origin and similar biological functions, i.e., Se-Zn. The study identified several quantitative trends in the demographic and medical characteristics of the participants. Alcohol users had significantly higher Zn concentrations than non-alcohol users; women had significantly higher Ca concentrations than men; higher BMI correlated positively with Cs and negatively with Be and Cr levels; and Cu, Sb, Cd, Se, and Ca concentrations increased with age. The presence of several toxic and potentially toxic elements was identified in the vitreous body: Al (>10 ppb); Cd, Cr, Pb, Ni, Mn; and Ba (<10 ppb); As, Hg, Sb, Tl, Bi, Be (<1 ppb). The study showed that, within a given geographic region, the accumulation profiles of toxic metals are quite homogeneous, indicating common sources of exposure. Full article

Sustainable management of vineyard residues through biochar production requires balancing carbon stability with agronomically relevant nutrient functionality. Pyrolysis temperature controls this trade-off by affecting carbon condensation and micronutrient availability. This study aimed to determine how pyrolysis temperatures (300 and 600 °C) govern this trade-off in vineyard-trimming biochars. The motivation focuses on optimizing carbon storage while maintaining micronutrient availability. Biochars were produced by slow pyrolysis at 300 and 600 °C for 1 h and characterized using proximate and elemental analyses, total macro- and micronutrient determination, and DTPA extraction to evaluate potentially bioavailable trace elements. The results showed that increasing temperature from 300 to 600 °C reduced yield (45.15 to 32.30%) and volatile matter (40.33 to 16.50%), while increasing fixed carbon from 55.37 to 77.33% and total carbon from 66.49 to 77.89%. Atomic ratios (H/C: 0.67 to 0.31; O/C: 0.32 to 0.18) confirmed enhanced carbon condensation at 600 °C. Regarding nutrients, although total Mn, Fe, Cu, and Zn concentrations declined at higher temperatures, their potentially bioavailable fractions (operationally defined as extractable with the chelating agent DTPA showed element-specific redistribution; Fe, Cu, and Zn extractability increased, while Mn decreased. These findings reveal a temperature-driven trade-off between carbon sequestration and micronutrient release. Full article

Despite global efforts to reduce landfill use for municipal waste, many sites remain active, and older closed sites still require management, particularly regarding leachate. Landfill leachate contains varying levels of organic and inorganic pollutants, generated through biological and physicochemical processes following water infiltration. Its complex composition—including COD, inorganic macro-components, heavy metals, and xenobiotics—necessitates effective treatment technologies to enable safe discharge into surface waters. This study compares low-cost, eco-sustainable adsorbents for the removal of ammonium, trace elements (Cd, Be, Fe, Cu, Ni, Pb, Cr, As, Sn, Sb, Se), and color (as an indirect measure of organic compounds) from urban landfill leachate. In more detail, six biochars from different biomass feedstocks and pyro-gasification conditions as well as natural chabazite and synthetic zeolite 13X (FAU-type) were investigated. After characterization, biochars were characterized and adsorption performance was assessed. Removal performance was comparatively evaluated after 24 h batch contact under fixed experimental conditions. Results showed that gasified biochars achieved high removal efficiency for metals and color but were ineffective for ammonium. Instead, both zeolites demonstrated efficient ammonium removal (~50%) but were less efficient for metals, reflecting the mechanism-driven selectivity of the adsorbents studied. Finally, a principal component analysis (PCA) revealed correlations between biochar physicochemical properties and contaminant retention, providing insight into key factors governing adsorption and informing the design of sustainable leachate treatment strategies. Full article

Maize silage can play a key role in policies aimed at stabilising local energy systems, as it constitutes a critical renewable feedstock for European biogas plants. By providing a dense and predictable source of chemical energy, it supports balance and reliability in the agricultural energy sector. To convert this potential into stable energy production, operators require kinetic models that translate routine silage quality indicators into concrete guidance for digester operation and control. Therefore, the aim of this article was to evaluate the batch kinetics of anaerobic digestion (AD) of maize silage and to select an adequate model for describing biochemical methane potential (BMP) profiles and associated energy recovery in the context of start-up, organic loading rate (OLR), hydraulic retention time (HRT) and feedstock preparation. Ten batches of silage (A–J) were examined, covering a realistic range of pH, electrical conductivity (EC), dry and volatile solids, ash, protein–fat–fibre fractions, fibre composition (NDF, ADF and ADL), derived fractions (hemicellulose, cellulose, and residual organic matter (OM)), C/N ratio and macro-/micronutrient profiles, including trace elements relevant to methanogenesis (Ni, Co, Mo, and Se). BMP tests were carried out in batch mode, and the resulting curves were fitted using the modified Gompertz and a first-order kinetic model. Methane yields of approx. 100–120 m³ CH₄/Mg fresh matter (FM) and 336–402 m³ CH₄/Mg volatile solids (VS), with CH₄ contents of 52–57% v/v, were typical for energy-grade maize silage. Kinetic and energetic behaviours were governed mainly by residual OM and hemicellulose (shortening the lag phase and increasing the maximum methane production rate), the ADL/cellulose ratio (controlling the slower hydrolytic tail), EC and Na/Cl/S (extending the lag phase), and C/N together with Ni/Co/Mo/Se (stabilising methanogenesis). The modified Gompertz model reproduced BMP curves with a pronounced lag phase and asymmetry more accurately (lower error and better information criterion values), and its parameters directly support start-up design, OLR ramp-up and energetic performance optimisation in bioenergy reactors. The novelty of this work lies in combining batch BMP tests, comparative kinetic modelling and detailed silage characterisation to establish quantitative links between kinetic parameters and routine maize silage quality indicators that are directly relevant for biogas plant operation and renewable energy production. Full article

Clementine, mandarin, and orange peels, which are usually discarded, can serve as promising, sustainable dietary supplements with beneficial compositions, as demonstrated in this study. Citrus peels are low in ash, fat, and protein, but high in moisture, fibre, sugar, and polyunsaturated fatty acids (PUFAs) (up to 60%). They contain high levels of omega-3 and omega-6 fatty acids, up to 30% each, making them a good health-promoting source, as shown by the values of nutritional indices as follows: PUFA/saturated fatty acid (SFA) (1.94 to 2.30), monounsaturated fatty acid (MUFA)/SFA (0.39 to 0.84), and PUFA/MUFA (2.37 to 5.82). Essential macro elements (K > Ca > Mg > S > P > Na) and trace elements (Fe > Zn > Mn > Cu > Cr > Mo > Co > Se) are unevenly distributed among the peels, along with non-essential elements, with Al (37 to 51 mg/kg) and Sr (17 to 30 mg/kg) predominating. Rare elements in food, such as V and W, are found up to 41 and 79 µg/kg respectively, followed by Nb > Ga > Y > Ge (5 to 11 µg/kg). Although citrus peels have a nutrient-dense composition, their monitoring must be ensured before inclusion in the common diet, particularly regarding non-essential elements, as for most of them the reference doses are not established and they could be harmful to human health. Full article

Thanks to its valuable nutritional value and captivating flavour, tahini, an oily paste made from sesame seeds, has recently become popular outside of Middle Eastern cuisine. However, alongside valuable and balanced levels of lipids, proteins, sterols, and minerals, this product may contain various contaminants, including toxic and potentially toxic elements. The aim of this study was therefore to evaluate the quality and safety of seven brands of commercial tahini. To this end, the proximate composition and the fatty acid and sterol profiles were determined. Moreover, the atherogenicity index (AI) and thrombogenicity index (TI) were also assessed. The elemental composition was screened, and the uptake percentage of each element was evaluated. The percentages of saturated (SFAs), mono- (MUFAs), and poly- (PUFAs) fatty acids fell within the following ranges, respectively: 15.44–17.14%, 37.93–43.36%, and 38.51–45.14%. The order of abundance of macro-elements for most samples was P > K > Ca > Mg > Na. Significant concentrations of essential trace elements were found in the tahini samples, including Zn, Fe, Mn, Cu and Se. As regards toxic elements, only one brand appears to exceed the maximum limits for Cd and Pb specified in the European Regulation. However, a low intake of most inorganic elements was obtained from the consumption of 1 g of tahini per day. Full article

The placenta, a temporary organ that connects mother and child for nutrient and metabolite exchange, becomes medical waste after birth but can provide valuable metabolic insights. Thirty-three placenta samples were analyzed using ICP-OES to determine concentrations of ten elements, including macro-, micro-, trace, and heavy metals. Results were compared with maternal and neonatal data, including Apgar scores, maternal age, and blood parameters. Correlations were found between elements (e.g., Ca–Mg, Fe–Zn, and Mn–Cu) and between mineral levels and maternal or infant parameters (e.g., Ca–RBC, Mn–Hb, Cu–PLT, and Cu–UA Pi). No quantifiable heavy metals were detected, nor associations with smoking, gestational diabetes, preterm birth, birth weight, or Apgar scores. Findings suggest that maintaining proper blood morphology and preventing anemia in pregnancy requires attention not only to iron but also to Ca²⁺, Mg²⁺, and Mn²⁺ levels. Manganese and copper assessment may be beneficial for diagnostic purposes in pregnant women. Further large-scale tissue studies are recommended, including comprehensive maternal–fetal health data such as Doppler velocimetry of placental vessels. Full article

Metallomics, which studies the role of metals in biological processes, is crucial for understanding the impact of elements on human health. It requires an integrated approach combining quantitative and functional methods, supported by advanced analytical techniques. A comprehensive understanding of metallomics considers the accumulation, speciation, and distribution of metals. In recent decades, an increasing number of studies have focused on determining metal levels in human tissues, particularly in the context of chronic diseases and developmental disorders. Levels of macro- and microelements, both essential and toxic, play a fundamental role in both physiological and pathological processes. Given the increasing health risks associated with addictions such as smoking, understanding the mechanisms of toxicity based on metallomic studies is crucial. This literature review synthesizes current advances in analytical techniques used to determine trace elements in biological samples, the accumulation of toxic metals, and the disruption of element homeostasis in tobacco smokers. The aim of this study is to identify key risks from tobacco-related metal exposure, thereby providing a deeper understanding of the long-term health consequences. The obtained results may constitute the basis for future directions of metallomic research. Full article

With rapid advancements in large language models for natural language processing, their role in semantic-aware process mining is growing. We study semantics-aware process mining, where decisions must reflect both event logs and textual rules. We propose an online, adaptive multi-agent framework that operates over a single knowledge base shared across three tasks—semantic next-activity prediction (S_NAP), trace-level semantic anomaly detection (T_SAD), and activity-level semantic anomaly detection (A_SAD). The approach has three key elements: (i) cross-task corroboration at retrieval time, formed by pooling in-domain and out-of-domain candidates to strengthen coverage; (ii) feedback-to-index calibration that converts user correctness/usefulness into propensity-debiased, smoothed priors that immediately bias recall and first-stage ordering for the next query; and (iii) stability controls—consistency-aware scoring, confidence gating with failure-driven query rewriting, task-level trust regions, and a sequential rule to select the relevance–quality interpolation. We instantiate the framework with Mistral-7B-Instruct, Llama-3-8B, GPT-3.5, and GPT-4o and evaluate it using macro-F1. Compared to in-context learning, our framework improves S_NAP, T_SAD, and A_SAD by 44.0%, 15.6%, and 7.1%, respectively, and attains the best overall profile against retrieval-only and correction-centric baselines. Ablations show that removing index priors causes the steepest degradation, cross-task corroboration yields consistent gains—most visibly on S_NAP—and confidence gating preserves robustness to difficult inputs. The result is immediate serve-time adaptivity without heavy fine-tuning, making semantic process analysis practical under drift. Full article

Growing health consciousness drives demand for convenient, nutrient-dense snacks. This study evaluates five Sukkari date-mixed-nut bar formulations (DNB1–DNB5; date/nut ratios 40:60–80:20) through comprehensive biochemical and nutritional analyses. Macronutrient profiling showed that higher date ratios increased moisture and carbohydrates, whereas higher nut ratios enhanced protein, fat, and caloric density. Mineral assays revealed progressive increases in calcium, phosphorus, magnesium, and trace elements as date content decreased. The assessment of phytochemicals and antioxidants demonstrated that total phenolics, flavonoids, and radical-scavenging activities peaked in nut-rich bars, declining by ~50% in date-rich bars, underscoring nuts’ dominant antioxidant role. HPLC profiling identified catechol and vanillic acid as the major phenolics, with optimal release and retention at the 60:40 ratio (DNB3). Amino acid (AA) analysis confirmed positive correlations between nut content and total/essential AAs; DNB1–DNB2 achieved favorable essential-to-nonessential AA ratios (0.56–0.59) and higher protein quality indices. Fatty acid (FA) composition analysis revealed that oleic acid was identified as the major constituent across all formulations, coupled with optimal omega-6/omega-3 ratios. GC-MS analysis identified a total of 31 volatiles, mainly benzene derivatives and FA methyl esters. Results also revealed that notable variations attributed to different date/nut ratios significantly alter aroma profiles, with DNB3 yielding the most remarkable diversity of health-associated volatiles. Results from PCA and hierarchical clustering suggest that a single dominant dimension (PC1, 94.47% variance) governs compositional differences among the five date bar formulations, reflecting deliberate variation in ingredient proportions. The evidence suggests that DNB3’s (60:40 Sukkari date to mixed nut ratio) delivers balanced macro-nutrients, robust antioxidants, and diverse bioactives, positioning it as a health-promoting functional snack, aligning with its suitability for athletes, clinical nutrition applications, and health-conscious populations. These findings support the commercial development of optimized date-nut bars as nutrient-dense functional snacks, and future work should focus on scale-up production, shelf-life stability, and assessing in vivo bioavailability. Full article

To investigate the geographical and species differences regarding mineral element content of camel milk, this research used camel milk from the Tacheng, Altay, and Ili regions of Xinjiang and cow milk, goat milk, and horse milk from the Tacheng region as subjects. The contents of 22 mineral elements were measured using inductively coupled plasma mass spectrometry (ICP-MS) and inductively coupled plasma optical emission spectrometry (ICP-OES). The results showed that the contents of macro elements Ca, P, K, and Na in camel milk were significantly higher than those in other milk sources (p < 0.01). The contents of trace elements such as Se, Sr, and Ni were very significantly higher than those in other milk sources (p < 0.01). The content of 12 mineral elements in camel milk was very significantly higher than in other types of milk (p < 0.01). Principal component analysis (PCA) and factor analysis emphasized the relationship between element distribution and different milk sources, and the linear discriminant analysis (LDA) model could identify the species type of milk. Geographical analysis indicated that trace elements such as Sr, Ni, and Cr were highly significantly enriched in Tacheng camel milk (p < 0.01). The established LDA model achieved traceability of the geographical origin of Xinjiang camel milk. This research reveals the mineral nutritional advantages of camel milk and its geographical differentiation patterns, providing theoretical support for exploring the functional properties of camel milk and for identifying species and regions through minerals. It is important to promote the upgrading of the specialty dairy product industry. Full article

This study focuses on the utilization of ash and slag waste from coal combustion for the production of ceramic construction materials. Detailed chemical and granulometric analyses were performed to determine the multicomponent composition of ash and slag, highlighting its dependence on particle size fractions. The macro- and microelement contents of fresh and aged ash and slag, as well as the coal fuel, were assessed. Significant amounts of SiO₂ (up to 54%), Al₂O₃ (27.5%), Fe₂O₃ (7%), and CaO (6.5%) were found, along with trace elements potentially hazardous to the environment, including Pb, Cu, Mo, and Y. Storage was shown to increase the concentrations of several elements (Pb, Cu, Ga, and Y) due to physicochemical weathering and pollutant migration. Based on comprehensive experimental data, criteria for evaluating ash and slag as raw materials were developed, and new qualitative and quantitative characteristics were identified, demonstrating their feasibility for use in construction material production. These results provide a foundation for systematic monitoring and environmentally responsible utilization of ash and slag waste. Full article