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22 pages, 3780 KiB  
Article
Taxonomic Diversity: Importance, Threats, and Status of Diatoms from Lowland Urban Springs (Northeast Poland)
by Wanessa Lewandowicz, Magdalena Grabowska, Agata Z. Wojtal, Katarzyna Puczko and Adam Więcko
Water 2025, 17(15), 2293; https://doi.org/10.3390/w17152293 (registering DOI) - 1 Aug 2025
Abstract
Springs are unique ecosystems found in lowland areas. In urban environments, these springs often have niches that are heavily transformed by human activity. In this study, we identified and compared the taxonomic diversity of diatom communities across various microhabitats—epilithon, epipelon, epipsammon, epibryon, and [...] Read more.
Springs are unique ecosystems found in lowland areas. In urban environments, these springs often have niches that are heavily transformed by human activity. In this study, we identified and compared the taxonomic diversity of diatom communities across various microhabitats—epilithon, epipelon, epipsammon, epibryon, and epixylon—within altered lowland urban springs. Our results revealed differences in diatom communities among the microhabitats, with the highest species richness observed in the epibryon. Notably, the presence of extremely rare species such as Amphora eximia, Caloneis aerophila, and Stauroneis muriella suggest that, even under urban conditions, springs continue to serve a refugial function for diatom diversity. These findings underscore the important role of urban springs in maintaining diatom diversity despite high anthropogenic pressure. We also assessed the ecological status of the springs using the Polish Multimetric Diatom Index (IO), which incorporates indicators of trophy, saprobity, and the abundance of reference species. All studied springs were classified as having very good ecological status. Full article
(This article belongs to the Special Issue Protection and Restoration of Freshwater Ecosystems)
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17 pages, 2032 KiB  
Article
The Impact of Hydrological Streamflow Drought on Pollutant Concentration and Its Implications for Sustainability in a Small River in Poland
by Leszek Hejduk, Ewa Kaznowska, Michał Wasilewicz and Agnieszka Hejduk
Sustainability 2025, 17(15), 6995; https://doi.org/10.3390/su17156995 (registering DOI) - 1 Aug 2025
Viewed by 62
Abstract
The paper presents the results of investigations into the relationship between selected water quality parameters and hydrological streamflow drought in a small river situated in the Mazovian Lowlands in Poland. As hydrological streamflow drought periods become more frequent in Poland, investigations about the [...] Read more.
The paper presents the results of investigations into the relationship between selected water quality parameters and hydrological streamflow drought in a small river situated in the Mazovian Lowlands in Poland. As hydrological streamflow drought periods become more frequent in Poland, investigations about the relationship between flow and water quality parameters can be an essential contribution to a better understanding of the impact of low flow on the status of water rivers. Data from a three-year study of a small lowland river along with significant agricultural land management was used to analyze the connection between low flows and specific water quality indicators. The separation of low-flow data from water discharge records was achieved using two criteria: Q90% (the discharge value from a flow duration curve) and a minimum low-flow duration of 10 days. During these periods, the concentration of water quality indicators was determined based on collected water samples. In total, 30 samples were gathered and examined for pH, suspended sediments, dissolved substances, hardness, ammonium, nitrates, nitrites, phosphates, total phosphorus, chloride, sulfate, calcium, magnesium, and water temperature during sampling. The study’s main aim was to describe the relation between hydrological streamflow droughts and chosen water quality parameters. The analysis results demonstrate an inverse statistically significant relationship between concentration and low-flow values for total hardness and sulfate. In contrast, there was a direct relationship between nutrient indicators, suspended sediment concentration, and river hydrological streamflow drought. Statistical tests were applied to compare the datasets between years, revealing statistical differences only for nutrient indicators. Full article
(This article belongs to the Topic Water Management in the Age of Climate Change)
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22 pages, 6172 KiB  
Article
Ethnomedicinal Properties of Wild Edible Fruit Plants and Their Horticultural Potential Among Indigenous Isan Communities in Roi Et Province, Northeastern Thailand
by Piyaporn Saensouk, Surapon Saensouk, Thawatphong Boonma, Auemporn Junsongduang, Min Khant Naing and Tammanoon Jitpromma
Horticulturae 2025, 11(8), 885; https://doi.org/10.3390/horticulturae11080885 (registering DOI) - 1 Aug 2025
Viewed by 144
Abstract
Wild edible fruit plants are integral to the cultural, nutritional, medicinal, and economic practices of Indigenous Isan communities in Roi Et Province, northeastern Thailand, a region characterized by plateau and lowland topography and a tropical monsoon climate. This study aimed to document the [...] Read more.
Wild edible fruit plants are integral to the cultural, nutritional, medicinal, and economic practices of Indigenous Isan communities in Roi Et Province, northeastern Thailand, a region characterized by plateau and lowland topography and a tropical monsoon climate. This study aimed to document the diversity, traditional uses, phenology, and conservation status of these species to inform sustainable management and conservation efforts. Field surveys and ethnobotanical interviews with 200 informants (100 men, 100 women; random ages) were conducted across 20 local communities to identify species diversity and usage patterns, while phenological observations and conservation assessments were performed to understand reproductive cycles and species vulnerability between January and December 2023. A total of 68 species from 32 families were recorded, with peak flowering in March–April and fruiting in May–June. Analyses of Species Use Value (0.19–0.48) and Relative Frequency of Citation (0.15–0.44) identified key species with significant roles in food security and traditional medicine. Uvaria rufa had the highest SUV (0.48) and RFC (0.44). Informant consensus on medicinal applications was strong for ailments such as gastrointestinal and lymphatic disorders. Economically important species were also identified, with some contributing notable income through local trade. Conservation proposed one species as Critically Endangered and several others as Vulnerable. The results highlight the need for integrated conservation strategies, including community-based initiatives and recognition of Other Effective area-based Conservation Measures (OECMs), to ensure the preservation of biodiversity, traditional knowledge, and local livelihoods. Full article
(This article belongs to the Section Medicinals, Herbs, and Specialty Crops)
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17 pages, 1204 KiB  
Article
The Great Wanderer: The Phylogeographic History of the Bicolor Pyramid Ant (Dorymyrmex bicolor Wheeler, 1906) in Central Veracruz, Mexico
by Maria Gómez-Lazaga and Alejandro Espinosa de los Monteros
Insects 2025, 16(8), 785; https://doi.org/10.3390/insects16080785 (registering DOI) - 31 Jul 2025
Viewed by 184
Abstract
The goal of phylogeography is to explain how microevolutionary forces shape the gene pool of a lineage into the geography. In this study we have evaluated the amount of genetic variation in 13 populations of Dorymyrmex bicolor distributed in a mountainous region in [...] Read more.
The goal of phylogeography is to explain how microevolutionary forces shape the gene pool of a lineage into the geography. In this study we have evaluated the amount of genetic variation in 13 populations of Dorymyrmex bicolor distributed in a mountainous region in Central Veracruz, Mexico. To do so, we sequenced fragments from the mitochondrial COI, COII, and nuclear LWRh genes. Segregated sites were found only at the mitochondrial markers, recovering a total of 21 different haplotypes. The nucleotide diversity ranged from 0 to 0.5% at the different sampling sites. Phylogenetic and spatial analyses of molecular variance revealed a weak but significant phylogeographic structure associated with lowland and mountainous zones. Molecular clock analysis suggests that radiation in the mountain area started 7500 years ago, whereas lineage radiation in the lowland started more recently, around 2700 years ago. The phylogeographic structure is incipient, with nests from lowlands more closely related to mountain nests than to other lowland nests, and vice versa. This seems to be consistent with a model of incomplete lineage sorting. The obtained patterns appear to be the result of restricted gene flow mediated by a complex topographic landscape that has been shaped by a dynamic geologic history. Full article
(This article belongs to the Special Issue Ant Population Genetics, Phylogeography and Phylogeny)
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14 pages, 1365 KiB  
Article
Molecular Genetic Basis of Reproductive Fitness in Tibetan Sheep on the Qinghai-Tibet Plateau
by Wangshan Zheng, Siyu Ge, Zehui Zhang, Ying Li, Yuxing Li, Yan Leng, Yiming Wang, Xiaohu Kang and Xinrong Wang
Genes 2025, 16(8), 909; https://doi.org/10.3390/genes16080909 - 29 Jul 2025
Viewed by 139
Abstract
Background: Complete environmental adaptation requires both survival and reproductive success. The hypoxic Qinghai-Tibet Plateau (>3000 m) challenges reproduction in indigenous species. Tibetan sheep, a key plateau-adapted breed, possess remarkable hypoxic tolerance, yet the genetic basis of their reproductive success remains poorly understood. [...] Read more.
Background: Complete environmental adaptation requires both survival and reproductive success. The hypoxic Qinghai-Tibet Plateau (>3000 m) challenges reproduction in indigenous species. Tibetan sheep, a key plateau-adapted breed, possess remarkable hypoxic tolerance, yet the genetic basis of their reproductive success remains poorly understood. Methods: We integrated transcriptomic and genomic data from Tibetan sheep and two lowland breeds (Small-tailed Han sheep and Hu sheep) to identify Tibetan sheep reproduction-associated genes (TSRGs). Results: We identified 165 TSRGs: four genes were differentially expressed (DEGs) versus Small-tailed Han sheep, 77 DEGs versus Hu sheep were found, and 73 genes were annotated in reproductive pathways. Functional analyses revealed enrichment for spermatogenesis, embryonic development, and transcriptional regulation. Notably, three top-ranked selection signals (VEPH1, HBB, and MEIKIN) showed differential expression. Murine Gene Informatics (MGI) confirmed that knockout orthologs exhibit significant phenotypes including male infertility, abnormal meiosis (male/female), oligozoospermia, and reduced neonatal weight. Conclusions: Tibetan sheep utilize an evolved suite of genes underpinning gametogenesis and embryogenesis under chronic hypoxia, ensuring high reproductive fitness—a vital component of their adaptation to plateaus. These genes provide valuable genetic markers for the selection, breeding, and conservation of Tibetan sheep as a critical genetic resource. Full article
(This article belongs to the Section Animal Genetics and Genomics)
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25 pages, 5461 KiB  
Article
Spaceborne LiDAR Reveals Anthropogenic and Biophysical Drivers Shaping the Spatial Distribution of Forest Aboveground Biomass in Eastern Himalayas
by Abhilash Dutta Roy, Abraham Ranglong, Sandeep Timilsina, Sumit Kumar Das, Michael S. Watt, Sergio de-Miguel, Sourabh Deb, Uttam Kumar Sahoo and Midhun Mohan
Land 2025, 14(8), 1540; https://doi.org/10.3390/land14081540 - 27 Jul 2025
Viewed by 328
Abstract
The distribution of forest aboveground biomass density (AGBD) is a key indicator of carbon stock and ecosystem health in the Eastern Himalayas, which represents a global biodiversity hotspot that sustains diverse forest types across an elevation gradient from lowland rainforests to alpine meadows [...] Read more.
The distribution of forest aboveground biomass density (AGBD) is a key indicator of carbon stock and ecosystem health in the Eastern Himalayas, which represents a global biodiversity hotspot that sustains diverse forest types across an elevation gradient from lowland rainforests to alpine meadows and contributes to the livelihoods of more than 200 distinct indigenous communities. This study aimed to identify the key factors influencing forest AGBD across this region by analyzing the underlying biophysical and anthropogenic drivers through machine learning (random forest). We processed AGBD data from the Global Ecosystem Dynamics Investigation (GEDI) spaceborne LiDAR and applied filtering to retain 30,257 high-quality footprints across ten ecoregions. We then analyzed the relationship between AGBD and 17 climatic, topographic, soil, and anthropogenic variables using random forest regression models. The results revealed significant spatial variability in AGBD (149.6 ± 79.5 Mg ha−1) across the region. State-wise, Sikkim recorded the highest mean AGBD (218 Mg ha−1) and Manipur the lowest (102.8 Mg ha−1). Within individual ecoregions, the Himalayan subtropical pine forests exhibited the highest mean AGBD (245.5 Mg ha−1). Topographic factors, particularly elevation and latitude, were strong determinants of biomass distribution, with AGBD increasing up to elevations of 2000 m before declining. Protected areas (PAs) consistently showed higher AGBD than unprotected forests for all ecoregions, while proximity to urban and agricultural areas resulted in lower AGBD, pointing towards negative anthropogenic impacts. Our full model explained 41% of AGBD variance across the Eastern Himalayas, with better performance in individual ecoregions like the Northeast India-Myanmar pine forests (R2 = 0.59). While limited by the absence of regionally explicit stand-level forest structure data (age, stand density, species composition), our results provide valuable evidence for conservation policy development, including expansion of PAs, compensating avoided deforestation and modifications in shifting cultivation. Future research should integrate field measurements with remote sensing and use high-resolution LiDAR with locally derived allometric models to enhance biomass estimation and GEDI data validation. Full article
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21 pages, 3397 KiB  
Article
Climate-Driven Habitat Shifts and Conservation Implications for the Submediterranean Oak Quercus pyrenaica Willd.
by Isabel Passos, Carlos Vila-Viçosa, João Gonçalves, Albano Figueiredo and Maria Margarida Ribeiro
Forests 2025, 16(8), 1226; https://doi.org/10.3390/f16081226 - 25 Jul 2025
Viewed by 1029
Abstract
Climate change poses a major threat to forests, impacting the distribution and viability of key species. Quercus pyrenaica Willd., a marcescent oak endemic to the Iberian Peninsula (Portugal and Spain) and southwestern France and a structural species in submediterranean forests, is particularly susceptible [...] Read more.
Climate change poses a major threat to forests, impacting the distribution and viability of key species. Quercus pyrenaica Willd., a marcescent oak endemic to the Iberian Peninsula (Portugal and Spain) and southwestern France and a structural species in submediterranean forests, is particularly susceptible to shifts in temperature and precipitation patterns. Aiming to assess its potential loss of suitable area under future climate scenarios, we developed high-resolution spatial distribution models to project the future habitat suitability of Q. pyrenaica under two climate change scenarios (SSP3-7.0 and SSP5-8.5) for the periods 2070 and 2100. Our model, which has an excellent predictive performance (AUC of 0.971 and a TSS of 0.834), indicates a predominantly northward shift in the potential distribution of the species, accompanied by substantial habitat loss in southern and lowland regions. Long-term potential suitable area may shrink to 42% of that currently available. This, combined with the limited natural dispersal capacity of the species, highlights the urgency of targeted management and conservation strategies. These results offer critical insights to inform conservation strategies and forest management under ongoing climate change. Full article
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21 pages, 4261 KiB  
Article
Seasonal Temperature and Precipitation Patterns in Caucasus Landscapes
by Mariam Elizbarashvili, Nazibrola Beglarashvili, Mikheil Pipia, Elizbar Elizbarashvili and Nino Chikhradze
Atmosphere 2025, 16(7), 889; https://doi.org/10.3390/atmos16070889 - 19 Jul 2025
Viewed by 722
Abstract
The Caucasus region, characterized by its complex topography and diverse climatic regimes, exhibits pronounced spatial variability in temperature and precipitation patterns. This study investigates the seasonal behavior of air temperature, precipitation, vertical temperature gradients, and inversion phenomena across distinct landscape types using observational [...] Read more.
The Caucasus region, characterized by its complex topography and diverse climatic regimes, exhibits pronounced spatial variability in temperature and precipitation patterns. This study investigates the seasonal behavior of air temperature, precipitation, vertical temperature gradients, and inversion phenomena across distinct landscape types using observational data from 63 meteorological stations for 1950–2022. Temperature trends were analyzed using linear regression, while vertical lapse rates and inversion layers were assessed based on seasonal temperature–elevation relationships. Precipitation regimes were evaluated through Mann-Kendall trend tests and Sen’s slope estimators. Results reveal that temperature regimes are strongly modulated by landscape type and elevation, with higher thermal variability in montane and subalpine zones. Seasonal temperature inversions are most frequent in spring and winter, especially in western lowlands and enclosed valleys. Precipitation patterns vary markedly across landscapes: humid lowlands show autumn–winter maxima, while arid and semi-arid zones peak in spring or late autumn. Some landscapes exhibit secondary maxima and minima, influenced by Mediterranean cyclones and regional atmospheric stability. Statistically significant trends include increasing cool-season precipitation in humid regions and decreasing spring rainfall in arid areas. These findings highlight the critical role of topography and landscape structure in shaping regional climate patterns and provide a foundation for improved climate modeling, ecological planning, and adaptation strategies in the Caucasus. Full article
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34 pages, 24111 KiB  
Article
Natural and Anthropic Constraints on Historical Morphological Dynamics in the Middle Stretch of the Po River (Northern Italy)
by Laura Turconi, Barbara Bono, Carlo Mambriani, Lucia Masotti, Fabio Stocchi and Fabio Luino
Sustainability 2025, 17(14), 6608; https://doi.org/10.3390/su17146608 - 19 Jul 2025
Viewed by 385
Abstract
Geo-historical information deduced from geo-iconographical resources, derived from extensive research and the selection of cartographies and historical documents, enabled the investigation of the natural and anthropic transformations of the perifluvial area of the Po River in the Emilia-Romagna region (Italy). This territory, significant [...] Read more.
Geo-historical information deduced from geo-iconographical resources, derived from extensive research and the selection of cartographies and historical documents, enabled the investigation of the natural and anthropic transformations of the perifluvial area of the Po River in the Emilia-Romagna region (Italy). This territory, significant in terms of its historical, cultural, and environmental contexts, for centuries has been the scene of flood events. These have characterised the morphological and dynamic variability in the riverbed and relative floodplain. The close relationship between man and river is well documented: the interference induced by anthropic activity has alternated with the sometimes-damaging effects of river dynamics. The attention given to the fluvial region of the Po River and its main tributaries, in a peculiar lowland sector near Parma, is critical for understanding spatial–temporal changes contributing to current geo-hydrological risks. A GIS project outlined the geomorphological aspects that define the considerable variations in the course of the Po River (involving width reductions of up to 66% and length changes of up to 14%) and its confluences from the 16th to the 21st century. Knowledge of anthropic modifications is essential as a tool within land-use planning and enhancing community awareness in risk-mitigation activities and strategic management. This study highlights the importance of interdisciplinary geo-historical studies that are complementary in order to decode river dynamics in damaging flood events and latent hazards in an altered river environment. Full article
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16 pages, 2323 KiB  
Article
Tree- and Stand-Scale Roost Selection and Partitioning by Bats Barbastella barbastellus Schreber, 1774 and Pipistrellus pygmaeus Leach, 1825 in a European Lowland Forest
by Alek Rachwald, Grzegorz Apoznański, Tomasz Oszako, Sandra Krzemińska, Ireneusz Ruczyński, Ewa Komar, Marcin Zegarek and Andrew Carr
Forests 2025, 16(7), 1189; https://doi.org/10.3390/f16071189 - 19 Jul 2025
Viewed by 309
Abstract
Our research focused on the roost preferences of two bat species in a forest environment. Throughout the Anthropocene, people have heavily altered the landscape. Forested habitation has declined, with remaining forests becoming fragmented and often deprived of old trees that provide shelter opportunities [...] Read more.
Our research focused on the roost preferences of two bat species in a forest environment. Throughout the Anthropocene, people have heavily altered the landscape. Forested habitation has declined, with remaining forests becoming fragmented and often deprived of old trees that provide shelter opportunities for bats. TReMs (tree-related microhabitats) are essential for forest-dwelling bat species as they provide an opportunity to roost and shelter. Following an infestation of Ips typographus L., the Białowieża Forest is saturated with dead spruce trees. We investigated roost selection in two forest-dwelling species, Barbastella barbastellus and Pipistrellus pygmaeus. To examine similarities or differences in roost selection between species, we radio-tracked 24 barbastelles and 13 soprano pipistrelles over three breeding seasons. We located a total of 48 barbastelle roosts and 15 pipistrelle roosts, together with the characteristics of the surroundings. We found that barbastelles select roosts almost exclusively in dead spruce trees (43/48), while pipistrelles selected roosts predominantly in live (n = 8) and dead (n = 5) broadleaved trees. Our results show that both bat species have clear differences in roost tree preference. In our study area, with an abundance of exfoliating bark, barbastelles showed a preference for roosting under flaking bark despite the availability of crevices within broadleaved trees. Our findings provide useful insight into forestry practices, highlighting the importance of standing dead trees. Full article
(This article belongs to the Special Issue Conservation and Restoration of Forest Biodiversity)
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20 pages, 3788 KiB  
Article
Assessing Forest Succession Along Environment, Trait, and Composition Gradients in the Brazilian Atlantic Forest
by Carem Valente, Renan Hollunder, Cristiane Moura, Geovane Siqueira, Henrique Dias and Gilson da Silva
Forests 2025, 16(7), 1169; https://doi.org/10.3390/f16071169 - 16 Jul 2025
Viewed by 372
Abstract
Tropical forests face increasing threats and are often replaced by secondary forests that regenerate after disturbances. In the Atlantic Forest, this creates fragments of different successional stages. The aim of this study is to understand how soil nutrients and light availability gradients influence [...] Read more.
Tropical forests face increasing threats and are often replaced by secondary forests that regenerate after disturbances. In the Atlantic Forest, this creates fragments of different successional stages. The aim of this study is to understand how soil nutrients and light availability gradients influence the species composition and structure of trees and regenerating strata in remnants of lowland rainforest. We sampled 15 plots for the tree stratum (DBH ≥ 5 cm) and 45 units for the regenerating stratum (height ≥ 50 cm, DBH < 5 cm), obtaining phytosociological, entropy and equitability data for both strata. Canopy openness was assessed with hemispherical photos and soil samples were homogenized. To analyze the interactions between the vegetation of the tree layer and the environmental variables, we carried out three principal component analyses and two redundancy analyses and applied a linear model. The young fragments showed good recovery, significant species diversity, and positive successional changes, while the older ones had higher species richness and were in an advanced stage of succession. In addition, younger forests are associated with sandy, nutrient-poor soils and greater exposure to light, while mature forests have more fertile soils, display a greater diversity of dispersal strategies, are rich in soil clay, and have less light availability. Mature forests support biodiversity and regeneration better than secondary forests, highlighting the importance of preserving mature fragments and monitoring secondary ones to sustain tropical biodiversity. Full article
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33 pages, 11613 KiB  
Article
Assessing and Mapping Forest Fire Vulnerability in Romania Using Maximum Entropy and eXtreme Gradient Boosting
by Adrian Lorenț, Marius Petrila, Bogdan Apostol, Florin Capalb, Șerban Chivulescu, Cătălin Șamșodan, Cristiana Marcu and Ovidiu Badea
Forests 2025, 16(7), 1156; https://doi.org/10.3390/f16071156 - 13 Jul 2025
Viewed by 562
Abstract
Understanding and mapping forest fire vulnerability is essential for informed landscape management and disaster risk reduction, especially in the context of increasing anthropogenic and climatic pressures. This study aims to model and spatially predict forest fire vulnerability across Romania using two machine learning [...] Read more.
Understanding and mapping forest fire vulnerability is essential for informed landscape management and disaster risk reduction, especially in the context of increasing anthropogenic and climatic pressures. This study aims to model and spatially predict forest fire vulnerability across Romania using two machine learning algorithms: MaxEnt and XGBoost. We integrated forest fire occurrence data from 2006 to 2024 with a suite of climatic, topographic, ecological, and anthropogenic predictors at a 250 m spatial resolution. MaxEnt, based on presence-only data, achieved moderate predictive performance (AUC = 0.758), while XGBoost, trained on presence–absence data, delivered higher classification accuracy (AUC = 0.988). Both models revealed that the impact of environmental variables on forest fire occurrence is complex and heterogeneous, with the most influential predictors being the Fire Weather Index, forest fuel type, elevation, and distance to human proximity features. The resulting vulnerability and uncertainty maps revealed hotspots in Sub-Carpathian and lowland regions, especially in Mehedinți, Gorj, Dolj, and Olt counties. These patterns reflect historical fire data and highlight the role of transitional agro-forested landscapes. This study delivers a replicable, data-driven approach to wildfire risk modelling, supporting proactive management and emphasising the importance of integrating vulnerability assessments into planning and climate adaptation strategies. Full article
(This article belongs to the Section Natural Hazards and Risk Management)
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20 pages, 6376 KiB  
Article
Analyses of MODIS Land Cover/Use and Wildfires in Italian Regions Since 2001
by Ebrahim Ghaderpour, Francesca Bozzano, Gabriele Scarascia Mugnozza and Paolo Mazzanti
Land 2025, 14(7), 1443; https://doi.org/10.3390/land14071443 - 10 Jul 2025
Viewed by 344
Abstract
Monitoring land cover/use dynamics and wildfire occurrences is very important for land management planning and risk mitigation practices. In this research, moderate-resolution imaging spectroradiometer (MODIS) annual land cover images for the period 2001–2023 are utilized for the twenty administrative regions of Italy. Monthly [...] Read more.
Monitoring land cover/use dynamics and wildfire occurrences is very important for land management planning and risk mitigation practices. In this research, moderate-resolution imaging spectroradiometer (MODIS) annual land cover images for the period 2001–2023 are utilized for the twenty administrative regions of Italy. Monthly MODIS burned area images are utilized for the period 2001–2020 to study wildfire occurrences across these regions. In addition, monthly Global Precipitation Measurement images for the period 2001–2020 are employed to estimate correlations between precipitation and burned areas annually and seasonally. Boxplots are produced to show the distributions of each land cover/use type within the regions. The non-parametric Mann–Kendall trend test and Sen’s slope are applied to estimate a linear trend, with statistical significance being evaluated for each land cover/use time series of size 23. Pearson’s correlation method is applied for correlation analysis. It is found that grasslands and woodlands have been declining and increasing in most regions, respectively, most significantly in Abruzzo (−0.88%/year for grasslands and 0.71%/year for grassy woodlands). The most significant and frequent wildfires have been observed in southern Italy, particularly in Basilicata, Apulia, and Sicily, mainly in grasslands. The years 2007 and 2017 experienced severe wildfires in the southern regions, mainly during July and August, due to very hot and dry conditions. Negative Pearson’s correlations are estimated between precipitation and burnt areas, with the most significant one being for Basilicata during the fire season (r = −0.43). Most of the burned areas were mainly within the elevation range of 0–500 m and the lowlands of Apulia. In addition, for the 2001–2020 period, a high positive correlation (r > 0.7) is observed between vegetation and land surface temperature, while significant negative correlations between these variables are observed for Apulia (r ≈ −0.59), Sicily (r ≈ −0.69), and Sardinia (r ≈ −0.74), and positive correlations (r > 0.25) are observed between vegetation and precipitation in these three regions. This study’s findings can guide land managers and policymakers in developing or maintaining a sustainable environment. Full article
(This article belongs to the Special Issue Integration of Remote Sensing and GIS for Land Use Change Assessment)
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22 pages, 2749 KiB  
Article
Genetic Diversity, Population Structure, and Historical Gene Flow Patterns of Nine Indigenous Greek Sheep Breeds
by Sofia Michailidou, Maria Kyritsi, Eleftherios Pavlou, Antiopi Tsoureki and Anagnostis Argiriou
Biology 2025, 14(7), 845; https://doi.org/10.3390/biology14070845 - 10 Jul 2025
Viewed by 430
Abstract
Ιn this study, we evaluated the genetic resources of nine Greek sheep breeds. The genotyping data of 292 animals were acquired from Illumina’s OvineSNP50 Genotyping BeadChip. The genetic diversity and inbreeding levels were evaluated using the observed and expected heterozygosity indices, the F [...] Read more.
Ιn this study, we evaluated the genetic resources of nine Greek sheep breeds. The genotyping data of 292 animals were acquired from Illumina’s OvineSNP50 Genotyping BeadChip. The genetic diversity and inbreeding levels were evaluated using the observed and expected heterozygosity indices, the FIS inbreeding coefficient, and runs of homozygosity (ROH). The genetic differentiation of breeds was assessed using the FST index, whereas their population structure was analyzed using admixture and principal components analysis (PCA). Historical recombination patterns and genetic drift were evaluated based on linkage disequilibrium, effective population sizes, and gene flow analysis to reveal migration patterns. PCA revealed distinct clusters mostly separating mountainous, insular, and lowland breeds. The FST value was the lowest between Serres and Karagouniko breeds (0.050). Admixture analysis revealed a genetic substructure for Serres and Kalarritiko breeds, while Chios, followed by Katsika, demonstrated the highest within-breed genetic uniformity. ROH analysis revealed low levels of inbreeding for all breeds. Genetic introgression from both Anatolia and Eastern Europe has been evidenced for Greek sheep breeds. The results also revealed that Greek sheep breeds maintain adequate levels of genetic diversity, without signs of excessive inbreeding, and can serve as valuable resources for the conservation of local biodiversity. Full article
(This article belongs to the Special Issue Genetic Variability within and between Populations)
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24 pages, 19652 KiB  
Article
How Do Natural Environmental Factors Influence the Spatial Patterns and Site Selection of Famous Mountain Temple Complexes in China? Quantitative Research on Wudang Mountain in the Ming Dynasty
by Yu Yan, Zhe Bai, Xian Hu and Yansong Wang
Land 2025, 14(7), 1441; https://doi.org/10.3390/land14071441 - 10 Jul 2025
Viewed by 237
Abstract
Ancient temple complexes in China’s mountainous landscapes exemplify a profound synthesis of environmental adaptation and cultural expression. This research investigates the spatial logic underlying the Wudang Mountain temple complex—a UNESCO World Heritage site—through integrated geospatial analysis of environmental factors. Using GIS-based modeling, GeoDetector, [...] Read more.
Ancient temple complexes in China’s mountainous landscapes exemplify a profound synthesis of environmental adaptation and cultural expression. This research investigates the spatial logic underlying the Wudang Mountain temple complex—a UNESCO World Heritage site—through integrated geospatial analysis of environmental factors. Using GIS-based modeling, GeoDetector, and regression analysis, we systematically assess how terrain, hydrology, climate, vegetation, and soil conditions collectively influenced site selection. The results reveal a clear hierarchical clustering pattern, with dense temple cores in the southwestern highlands, ridge-aligned belts, and a dominant southwest–northeast orientation that reflects intentional alignment with mountain ridgelines. Temples consistently occupy zones with moderate thermal, hydrological, and vegetative stability while avoiding geotechnical extremes such as lowland humidity or unstable slopes. Regression analysis confirms that site preferences vary across temple types, with soil pH, porosity, and bulk density emerging as significant influencing factors, particularly for cliffside temples. These findings suggest that ancient temple planning was not merely a passive response to sacred geography but a deliberate process that actively considered terrain, climate, soil, and other environmental factors. While environmental constraints strongly shaped spatial decisions, cultural and symbolic considerations also played an important role. This research deepens our understanding of how environmental factors influenced the formation of historical landscapes and offers theoretical insights and ecologically informed guidance for the conservation of mountain cultural heritage sites. Full article
(This article belongs to the Special Issue Natural Landscape and Cultural Heritage (Second Edition))
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