Search Results (252)

Root rot disease severely threatens tropical fruit production, leading to plant mortality and reduced yields; however, the mechanisms of host defense responses and pathogen infection remain poorly understood. In this study, Fusarium acutatum was isolated from diseased Annona squamosa roots and identified through morphological features and ITS phylogeny (99.8% identity). Infection triggered a marked activation of antioxidant defenses, with elevated POD, SOD, PAL, PPO, and CAT activities. Transcriptomic and TMT-based quantitative proteomic analyses identified 23,791 and 74,403 differentially expressed genes (DEGs) and 367 and 609 differentially expressed proteins (DEPs) in root at 5 and 10 days post inoculation, respectively, relative to the control. These DEGs and DEPs were consistently enriched in pathways involving redox regulation, protein synthesis and processing, ubiquitin-mediated proteolysis, phenylpropanoid and flavonoid metabolism, cell wall remodeling, plant–pathogen interaction and MAPK signaling. Integrated transcriptomic–proteomic correlation analysis showed clear positive associations between key defense-related genes and proteins, suggesting that phenylpropanoid metabolism and reactive oxygen species (ROS) scavenging play central roles in resistance. Key genes such as CHI2, CHS, and CYP were strongly induced and validated by qPCR, supporting coordinated activation of the defense systems. Furthermore, F. acutatum exhibited upregulation of 50 pathogenic-related proteins, including 4 cell wall-degrading enzymes (e.g., CBH1, pectate lyase), 5 metabolic regulation or signal transduction enzymes (e.g., gabD, TPI, and ENO) and 3 potential effectors, suggesting coordinated pathogen strategies for host colonization. Collectively, this study provides comprehensive multi-omics insight into the molecular mechanisms underlying A. squamosa defense against F. acutatum and offers candidate targets supported by omics evidence, serving as a theoretical reference for the management of root rot. Full article

Urban health, well-being, and equity—core objectives of Sustainable Development Goals (SDGs 3, 10, and 11)—have become key themes in contemporary urban planning research and landscape research. While existing studies focus predominantly on quantitative assessment, environmental exposure, and human mobility, the historical origins of health-oriented urban green space planning remain insufficiently explored. Focusing on Lingnan area as a representative case, this research investigates the emergence of public green space in late Qing cities and its early contributions to urban health and spatial governance. Through a systematic examination of American and British Gardens at the Thirteen Factories in Guangzhou, the planned public green space system of the Shameen concession, and the municipal greening practices of neighboring Hong Kong and Macao, the study further analyzes Zhang Zhidong’s tree-lined boulevard project along Changdi avenue as a key instance of localized institutional adaptation. Drawing on late-Qing and Republican newspapers, nineteenth-century Western travelogs and reports, historical and contemporary studies and photo albums, the study finds the following: (1) the American and British Gardens marked the earliest emergence of health-oriented urban green space in Lingnan area; (2) the systematically planned green space network of the Shameen concession constituted a prototypical form of health-oriented urban green space planning; (3) the botanical gardens, street-tree systems, public parks, and institutionalized management practices in Hong Kong and Macao exerted a strong regional demonstrative influence on Guangzhou; (4) the street-tree planting along Changdi Avenue represented a localized absorption of foreign planning paradigms and marked the institutionalization of municipal greening in Guangzhou. Although these early practices did not yet form a modern healthy city planning framework at that time, they played a crucial role in improving urban sanitation, enhancing public space quality, and shaping urban order. By tracing the historical trajectory from transnational demonstration to local adaptation and institutional consolihdation, this study provides new insights into the historical foundations of health-oriented urban planning in China and contributes a long-term perspective to contemporary debates on healthy cities and nature-based urban interventions. Full article

We investigate the effect of financial openness on corporate resilience. Corporate resilience metrics refer to the processes through which firms respond to crises, encompassing the capabilities developed during adaptation, absorption, innovation, recovery, and development. Using dynamic difference-in-differences (DID) models and panel data on Chinese A-share listed firms from 2009 to 2022, we found that firms included in the Shanghai-Hong Kong and Shenzhen-Hong Kong Stock Connect (SSHK) lists exhibited higher levels of corporate resilience after the openness. Introducing foreign ownership and improving the quality of information disclosure are two plausible pathways through which financial openness can promote corporate resilience. At the same time, the degree of industry competition and level of external financing dependence moderate the results. Importantly, corporate resilience moderates the positive long-term effect of financial openness on firms’ total factor productivity (TFP). These findings highlight that fostering corporate resilience is not merely an outcome but a critical condition for translating financial integration into sustainable productivity gains, enlightening resilience-oriented policymaking in emerging markets undergoing reform. Full article

Understanding how long-term local climate zone (LCZ) dynamics interact with rapid urbanization and land surface temperature (LST) changes is essential for sustainable planning in megaregion-scale urban clusters. In this paper, we propose a multi-feature local sample transfer method to obtain LCZ maps from 2000 to 2020 in the Guangdong–Hong Kong–Macao Greater Bay Area (GBA) and then analyze spatiotemporal changes in LCZs and their impacts on surface thermal environments. Results show the following: (1) The proposed multi-feature local sample transfer approach significantly improves the efficiency of long-term LCZ mapping by greatly reducing the effort required for sample acquisition. (2) The built types (LCZ1–10) increased by 1.34% overall, with large low-rise (LCZ8) showing the greatest expansion (4.72%). The compact low-rise (LCZ3) was the only built type to decline, decreasing by 2.02%. (3) Urbanization has produced a contiguous warming core that expands outward from the central metropolitan zones, thereby promoting the UHI coalescence. (4) Dense trees (LCZA) and large low-rise (LCZ8) exerted the strongest influence on LST. Large low-rise (LCZ8) consistently exhibited the highest warming contribution in Foshan, Zhongshan, and Dongguan. In coastal cities including Shenzhen, Hong Kong, and Macao, the largest LST increases occurred when water (LCZG) areas were converted to bare rock or paved (LCZE) or cs (LCZ1–10). Overall, the results highlight the strong coupling between urbanization and surface heating, providing critical insights for urban climate adaptation and integrated land-use planning in rapidly urbanizing megaregions. Full article

Rat hepatitis E virus (rat HEV, Rocahepevirus genotype C1) represents a potential zoonotic threat, but its epidemiological and evolutionary characteristics in small mammals remain poorly understood, especially in regions with complex geography. Between 2022 and 2024, we collected 818 small mammals from seven border counties and cities in Yunnan, China. Rat HEV RNA was detected by RT-PCR, risk factors were assessed using binary logistic regression, and full genomes were sequenced for phylogenetic and molecular clock analysis. The overall prevalence of rat HEV was 6.23% (51/818), with significantly higher odds observed in Gengma and Heqing counties, in oriental house rat (Rattus tanezumi) and Chevrieri’s field mouse (Apodemus chevrieri), in residential habitats, and at mid-high altitudes (all p < 0.001). The 51 partial genomic sequences (RdRp gene) obtained in this study clustered within Rocahepevirus, forming two distinct subclades associated with host species. The two complete genomes, GS188 and GS197 from Rattus tanezumi, were classified as subtypes C1b and C1d, respectively. Bayesian analysis estimated that GS197 diverged from a closely related Rattus tanezumi-derived strain around 1998, while GS188 diverged from a lineage containing shrew and human strains around 1931. These findings reveal a relatively high prevalence and substantial genetic diversity of Rochepevirus in southwestern Yunnan, suggesting human-influenced transmission dynamics and a potential for cross-species infection. Full article

Insulin, a pivotal hormone synthesized by the pancreas and regulated through hepatic first-pass metabolism, plays an essential role in the management of diabetes. However, non-therapeutic exposure to insulin can lead to life-threatening hypoglycemia. The postmortem diagnosis of fatalities resulting from exogenous insulin presents numerous forensic challenges, including the disruption of pharmacokinetic evidence due to the rapid degradation of insulin after death and the lack of pathognomonic histopathological markers. These factors create significant obstacles in establishing medicolegal causality. Furthermore, the mechanisms underlying insulin overdose-induced injury to the pancreas and liver are poorly understood. This study aims to address these gaps by integrating standardized histopathology, precision laser microdissection, and advanced proteomics to systematically profile the global proteome and phosphoproteome of the liver and pancreas. Furthermore, it includes spatially resolved proteomic mapping of pancreatic microcompartments (islets versus acini) in models of insulin overdose. Comparative analysis with controls revealed dysregulated proteins and phosphorylation sites, along with perturbations in metabolic pathways, primarily affecting pancreatic exocrine and hepatic function. Cross-organ comparative analysis elucidated organ-specific alterations in proteins and phosphorylation sites, uncovering core functional perturbations in these vital organs. In conclusion, this study presents a multi-level proteomic resource that profiles insulin-overdosed rat models and provides insights into the core pathological and molecular signatures. Full article

Background: Population ageing is a global challenge, prompting ageing-in-place policies in Hong Kong to support community-dwelling older adults while reducing healthcare costs. Yet, their impact on health-related quality of life (HRQoL) remains underexplored amid Hong Kong’s long life expectancy and growing older population. Traditional surveys are costly and time-consuming, while routinely collected registration data offers a large, efficient source for health insights. This study uses enhanced administrative data to track HRQoL trajectories and inform policy. Methods: This is a prospective, open-ended longitudinal study, enrolling adults aged 50 or older from a collaborating non-governmental organization in Hong Kong’s Southern District. Data collection, started in February 2021, occurs annually via phone and face-to-face interviews by trained social workers and volunteers using a standardized questionnaire to assess individual (e.g., socio-demographics), environmental (e.g., social support via Lubben Social Network Scale-6), biological (e.g., chronic illnesses), functional (e.g., cognition via Montreal Cognitive Assessment), and HRQoL (e.g., EQ-5D-5L) factors. A secure online system links health and service use data (e.g., service utilization like community care visits). Analysis employs descriptive statistics, group comparisons, correlations, growth modelling to identify health trajectories, and structural equation modelling to test a revised quality-of-life framework. Sample size (projected 470–580 after two follow-ups from a 2321 baseline) is based on power calculations: 300–500 for latent class growth analysis (LCGA) class detection and 200–400 for structural equation modelling (SEM) fit (e.g., RMSEA < 0.06) at 80% power/α = 0.05, simulated via Monte Carlo with a 50–55% attrition. Discussion: This is the first longitudinal HRQoL study in Hong Kong using enhanced non-governmental organization (NGO) administrative data, integrating social–ecological and HRQoL models to predict trajectories (e.g., stable vs. declining mobility) and project care demands (e.g., increase in in-home care for frailty). Unlike prior cross-sectional or inpatient studies, it offers a scalable model for NGOs, informing ageing-in-place policy effectiveness and equitable geriatric care. Full article

Quantifying the regional source of long-lived ozone precursors (especially GHGs) transported to Hong Kong is hampered by sparse observational data and computational limitations. This study introduces an observation-driven analytical framework that integrates a tracer ratio (ethylbenzene/m,p-xylene), wind–source–distance correlations to constrain transport corridors, and inventory mapping to determine the province- and sector-specific contributions, operationalized by identifying transport periods from observations, classifying sources with diagnostic ratios into five emission categories, deriving seasonal weighting factors via frequency normalization, mapping high-resolution inventory classes to these categories to restructure sectoral inventories, and combining normalized provincial spatial weights with the restructured inventories to quantify cross-boundary CO₂ and CH₄ emissions by sector and region. High-resolution measurements were conducted at the Cape D’Aguilar Supersite (CDSS), which showed dominant wintertime regional transport with mean concentrations of 435.29 ± 7.64 ppm (CO₂) and 2083.45 ± 56.50 ppb (CH₄). Thirteen transport periods were quantitatively analyzed, and province–sector contributions were estimated. The dominant provincial contributors were Guangdong (20.66%), followed by Jiangxi (18.36%) and Zhejiang (11.15%). Motor vehicles (70%), fuel combustion (15%), and solvent use (10%) were the primary contributing sectors. The framework enables province- and sector-specific attribution under stated assumptions and provides a tool for measuring cross-boundary mitigation and developing air quality and climate strategies in monsoon-affected coastal cities. Full article

Early diagnosis of pediatric flatfoot is critical to provide effective and timely orthotic intervention to prevent long-term complications. Current methods like manual measurements or three-dimensional (3D) scanning are often not ideal for rapid screening. This study addresses this gap by developing a regression model to predict foot arch height (AH) among Chinese primary schoolchildren by using simple two-dimensional (2D) footprint parameters, thus enabling orthosis prescription in an accessible and timely manner. A correlational study is conducted with 57 Hong Kong children who are 8–12 years old with flatfeet. Anthropometric footprint measurements, including four footprint indexes, Clarke’s Angle (CA), Chippaux–Smirak Index (CSI), Staheli Index (SI), and Sztriter–Godunov index (KY) are obtained from 2D footprints, whereas the arch height (AH) is measured by a caliper. One-way ANOVA is used to identify the measurements associated with flatfoot severity. A stepwise regression analysis is also used to determine the key footprint predictors of AH. The results show that the footprint indexes, heel width, ball width, medial ball length, arch depth and arch breadth significantly differentiated flatfoot severity. The stepwise regression model explains 44% of the variance by identifying the CA, heel width (HW) and arch depth (AD) as the strongest predictors of AH. The CA, HW and AD measured from the 2D footprints can serve as practical predictors of AH in Chinese flatfooted children. This study offers a transformative, accessible tool for pediatric flatfoot screening. Unlike resource-intensive 3D scanning or clinical assessments, our approach uses simple 2D footprints to estimate AH. This enables rapid, preliminary screening in diverse settings, empowering individuals to identify potential issues and seek timely professional evaluation. By democratizing access to early detection, it streamlines referrals, reduces unnecessary complex assessments, and facilitates earlier, personalized orthotic intervention for improved long-term foot health. Full article

In recent years, deep learning-based methods for surface defect detection in steel strips have advanced rapidly. Nevertheless, existing approaches still face several challenges in practical applications, such as insufficient dimensionality of feature information, inadequate representation capability for single-modal samples, poor adaptability to few-shot scenarios, and difficulties in cross-domain knowledge transfer. To overcome these limitations, this paper proposes a multi-modal fusion framework based on graph neural networks for few-shot classification and detection of surface defects. The proposed architecture consists of three core components: a multi-modal feature fusion module, a graph neural network module, and a cross-modal transfer learning module. By integrating heterogeneous data modalities—including visual images and textual descriptions—the method facilitates the construction of a more efficient and accurate defect classification and detection model. Experimental evaluations on steel strip surface defect datasets confirm the robustness and effectiveness of the proposed method under small-sample conditions. The results demonstrate that our approach provides a novel and reliable solution for automated quality inspection of surface defects in the steel industry. Full article

Objective: Hexafluoropropylene oxide dimer acid (HFPO-DA), also known as GenX, is widely used globally, raising concerns about its safety and public health implications. However, its toxicity mechanism remains unclear. The purpose of this study was to develop a reliable method for detecting HFPO-DA in mice and to investigate its absorption, distribution, and impact on hepatic lipid metabolism. Method: HFPO-DA levels were measured in the serum and eight tissues of C57BL/6J mice after oral administration using ultra-performance liquid chromatography–tandem mass spectrometry (UPLC-MS/MS). Lipid metabolites in the liver were also detected and analyzed. Results: HFPO-DA was rapidly absorbed into the bloodstream and widely distributed throughout all tested tissues. It penetrated the blood–brain barrier, with the highest concentration in the liver; however, long-term effects on the lungs also warrant attention. HFPO-DA disrupted liver lipid metabolism, leading to acylcarnitine accumulation while lowering triglycerides and cholesterol. Conclusion: This study on the pharmacokinetics and tissue distribution of HFPO-DA in mice following oral exposure revealed that HFPO-DA exacerbates liver injury by altering hepatic lipid metabolism. These findings provide theoretical support for toxicological studies on the emerging environmental pollutant HFPO-DA. Full article

Glycerol Dialkyl Glycerol Tetraethers (GDGTs) are microbial membrane lipids that can provide crucial information for identifying organic carbon sources and understanding paleoenvironments. Despite numerous studies reporting the presence of GDGTs in various terrestrial and marine environments, there is a paucity of reports concerning GDGTs in mangrove wetlands that are characterized by unique hydrological conditions and disproportionately high accumulation rates of blue carbon (i.e., carbon sequestered in coastal ecosystems, where tidal flooding and anaerobic sediments facilitate exceptional long-term carbon storage). This study investigates GDGTs in 81 sediment samples from 5 sediment cores collected from three Asian mangrove wetlands in Bangladesh, Hong Kong, and Guangxi Province, China. The Hong Kong mangrove sediments had the highest GDGT concentration (370.18 ± 58.00 ng·g⁻¹ dws), followed by Bangladesh mangrove sediments (136.70 ± 41.70 ng·g⁻¹ dws), while Guangxi mangrove sediments had the lowest (100.80 ± 28.71 ng·g⁻¹ dws). All samples demonstrated high BIT index values (>0.8), low IIIa/IIa index values (0.09–0.19) and the predominance of tetramethylated brGDGTs (70.38 ± 2.21%), indicating that terrestrial inputs are the primary source of organic carbon. Despite overall low methylation index (MI) values (0.15–0.35) and GDGT-0/Cren ratios, deeper sediment samples in the lower part of HK exhibited GDGT-0/Cren > 2, likely reflecting enhanced contributions of methanogenic archaea under distinct redox conditions compared to upper sediments. This in situ production may complicate the application of GDGT-based paleo-proxies, as indicated by the substantial deviations between CBT’-pH (MBT’_5ME-temperature) and measured pH (instrumental temperature). The dominant bacterial phyla in the mangrove sediments of Guangxi and Bangladesh were Proteobacteria, Actinobacteriota, Chloroflexi, Acidobacteriota, and Firmicutes (>70% relative abundance). However, correlations between microbial community compositions and brGDGT isomers are different among sampling sites. Our study emphasizes that site- and depth-specific microbial activity may significantly contribute to organic matter cycling and the in situ production of GDGTs in mangrove sediments. These factors should be taken into account for organic carbon sequestration and the validity of GDGT-based paleo-proxies in mangrove wetlands. Full article

Range height indicator (RHI) scans are performed routinely by the long-range Doppler Light Detection and Ranging (LIDAR) systems at the Hong Kong International Airport (HKIA). This paper presents some novel observations of the airflow in the airport region by RHI scans that have not been reported in the literature in the past—namely, areas of reverse flow and vortex shedding in east to southeasterly winds; severe windshear in the easterly; a converging southerly flow; a descending northeasterly jet; and the undercutting of the southwesterly flow by sea breeze. Many of these flow features are associated with low-level windshear, supported by pilot windshear reports, and thus their observations have practical applications. The technical feasibility of forecasting these airflow features is also studied in this paper, and it is found that large eddy simulations based on a mesoscale meteorology model manage to capture these wind features most of the time, but the simulated headwind change is generally slightly smaller than observed. The results in this paper have application value for windshear alerting and forecasting for an airport situated in an area of complex terrain, and they should be of interest for further studies of mountain meteorology. Full article

Eczema is the most common skin disease among Hong Kong’s adults and children, affecting an estimated 30% of the total population. Western and Chinese medicinal ointments are the usual treatment for eczema. Conventional Western medicinal ointments are topical corticosteroids and non-steroidal agents. Eczema skin products include “Aveeno Parabens Lotion”, “Cerave Moisturizing Cream”, and “Cetaphil Lotion”. However, these are not a long-term solution for managing significant erythema. Chinese medicinal ointments are based on adjusting the formula, including the ingredients and amount, to address an individual’s skin condition and other factors that may be worsening symptoms. This approach aims to regulate the immune system and make it less reactive to environmental and food allergies. This approach is mainly for local topical use. The ingredients of eczema skin products should include Coptis chinensis Franch, Phellodendron chinense Schneid, Angelica sinensis (Oliv.) Diels, Rehmannia glutinosa Libosch, Curcuma longa L., and sesame oil. Chinese medicinal ointments are natural ingredients, personalized formulas, and concerned with holistic healing, while Western medicinal ointments provide fast-acting relief, targeted action, and a standardized dosage. Methods: Nine electronic databases, such as WanFang Data, PubMed, Science Direct, Scopus, Web of Science, Springer Link, SciFinder, and the China National Knowledge Infrastructure (CNKI), were searched mainly within the past twenty years and without any language restrictions. The inclusion criteria were the keywords “Western medicine and ointment”, “Chinese medicine and ointment”, and “Western and Chinese medicines and ointment”. Differences in effectiveness between Western and Chinese ointments were evaluated to determine if they had functions against eczema. This review included an analysis and summary of all relevant papers. Results: Western medicinal ointments are topical corticosteroids, and they exert their pharmacological activities via many mechanisms, including anti-inflammatory, immunosuppressive, antiproliferative, and vasoconstrictive effects on eczema. Similarly, Chinese medicinal ointments have the same pharmacological functions, but they may focus on the immune system for the treatment of inflammatory and skin conditions, including erythema, edema, dryness, desquamation, and callus exfoliation. Conclusion: Based on the clinical research, the effectiveness rate of integrated Chinese and Western medicines was 88%, which was greater than the 70% rate for using Western medicine alone to treat eczema. Western and Chinese medicinal ointments have different active ingredients with advantages and disadvantages for eczema or when acting as skin care products. The most important thing is knowing “How” to use Western and Chinese medicinal ointments properly, especially for some formulations of Chinese ointments. It may be beneficial to consider the pharmacokinetic studies of herbal ingredients, which offer personalized formulas tailored to individual body constitutions and conditions, as well as to emphasize holistic healing, addressing both symptoms and underlying imbalances in the body. Much more work needs to be carried out, such as safety assessments of these ointments for use as skin care products for eczema. Full article

In the design of operating procedures, structures, and control systems for agricultural machinery and equipment, it is necessary to fully consider data on the properties of relevant agricultural materials as the basis for research and design. Therefore, studying the physical properties of agricultural materials is of great significance. The basic physical parameters of agricultural materials include their shape, size, density, porosity, and moisture content. This study focuses on the triaxial dimensions, 1000-grain weight, moisture content, and tribological properties (sliding friction angle, natural repose angle) of the seeds of 16 varieties of small-seeded vegetables commonly grown in Hainan, including flowering Chinese cabbage, Chinese cabbage, lettuce, and leaf lettuce. Measurements were conducted using instruments such as a digital vernier caliper (Deli, Ningbo, China; accuracy 0.01 mm), an electronic balance (LICHEN, Shanghai, China; accuracy 0.001 g), a constant-temperature oven (Shangyi, Shanghai, China), and self-developed sliding friction angle and natural repose angle testers. Discrete element simulations were performed via EDEM 2021 software to validate the tribological properties by establishing particle models (spherical for flowering Chinese cabbage and Chinese cabbage; long–flat for lettuce and leaf lettuce) and instrument geometric models. Additionally, seed germinability (germination potential, germination rate, and germination speed) was tested using a constant-temperature incubation method. The results showed distinct differences between near-spherical and long–flat seeds in geometric characteristics, 1000-grain weight (2.27–3.06 g vs. 1.00–1.29 g), and tribological behavior (e.g., smaller natural repose angles for near-spherical seeds indicating better flowability). Plastic plates were identified as optimal for seed box guides due to lower sliding friction coefficients. EDEM 2021 simulations effectively verified the experimental data. High-germination-rate seeds (e.g., Hong Kong flowering Chinese cabbage, and Lifeng No.3 Chinese cabbage) were recommended for subsequent trials. These findings provide data support for the selection, design, and optimization of seed rope braiding machine components and sustainable seeding process. Full article