Search Results (3,459)

The physiological relevance of in vitro models is limited because conventional two-dimensional cell culture systems are unable to replicate the structural and functional complexity of native tissues. Extracellular matrix (ECM)-mimetic hydrogels have become important platforms for tissue engineering applications. This work developed hybrid hydrogels that mimic important biochemical and mechanical characteristics of cardiac tissue by combining decellularized bovine pericardium-derived (dBP) ECM, gellan gum (GG), and spermine (SPM). Although dBP offers tissue-specific biological cues, processing compromises its mechanical integrity. This limitation was overcome by adding GG, whose ionic gelation properties were optimized using DMEM and SPM. The hydrogels’ mechanical, biological, physicochemical, and structural characteristics were all evaluated. Under physiologically simulated conditions, the formulations showed quick gelation and long-term stability; scanning electron microscopy revealed an interconnected, ECM-like porous microarchitecture. While uniaxial compression testing provided Young’s modulus values comparable to native myocardium, rheological analysis revealed a concentration-dependent increase in storage modulus with increasing SPM content. H9C2 cardiomyoblasts were used in cytocompatibility studies to confirm that cell viability, morphology, and cytoskeletal organization were all preserved. All of these findings support the potential application of dBP−GG−SPM hydrogels in advanced in vitro cardiac models by showing that they successfully replicate important characteristics of cardiac ECM. Full article

Smart agricultural systems need stable thermal environments for greenhouses, livestock housing, and on-farm processing. However, renewable heat sources such as solar collectors and heat pumps often cause fluctuations that challenge reliable operation. Thermal energy storage (TES)—particularly water-based sensible tanks, stratified reservoirs, and phase-change material (PCM) systems—provides an effective solution by decoupling heat supply and demand. In this review, tank-based TES technologies for agricultural applications, focusing on design, integration with renewable energy systems, and control strategies, are critically examined. Key performance aspects, including thermal stratification, state-of-charge estimation, and advanced predictive control, are analyzed to identify best practices and limitations. The review finds that sensible TES remains dominant in farm applications due to its low cost and durability, while latent (PCM/ice) and thermochemical storage provide a higher energy density and long-duration potential but are presently limited by material stability, system complexity, and cost. From an environmental perspective, TES contributes to reducing fossil fuel dependence, improving resource efficiency, lowering greenhouse gas emissions, and boosting the resilience of rural farming systems. Overall, TES is recognized as a key enabling technology for climate-smart, energy-efficient, and sustainable agricultural operations. However, remaining research gaps include long-term field validation, standardized performance metrics, and life-cycle environmental assessment. Full article

In provincial electricity markets where long-term contracts and spot trading coexist, multiple categories of imbalance funds arise from congestion, energy deviations and dual-track price differences, posing challenges to energy management systems (EMS) in terms of fair and robust settlement. This paper proposes an EMS-oriented framework to assess and diagnose alternative imbalance settlement mechanisms in a provincial dual-track market. First, a unified settlement model is developed that reconstructs key imbalance fund categories and allocates them to heterogeneous agents—thermal, renewable and storage units and different user groups—under a library of settlement rules. Second, a multi-scenario simulation platform is built, covering normal operation, tight supply and high-renewable-volatility conditions. Third, a multi-criteria evaluation scheme is designed to quantify economic efficiency, fairness, risk and renewable support for each mechanism–scenario combination. Finally, a category–agent two-dimensional diagnostic module is introduced to reveal misallocation patterns and the main money-transfer paths among fund categories and agent groups. A case study on a realistic provincial system shows that the proposed framework can distinguish mechanisms with better overall robustness, identify severe cross-subsidies in extreme scenarios and provide practical guidance for refining imbalance settlement parameters within EMS-driven market operations. Full article

We report on the study of the immobilization process of non-radioactive rhenium (Re), a chemical analogue of technetium-99 (⁹⁹Tc), in compounds based on magnesium potassium phosphate (MKP), as well as the possibility of enhancing their properties with iron-bearing additives/fillers. Powdered Re₂O₇ was used as the initial Re-containing source. Because of the solubility and high leachability of Tc (VII), which is also volatile at high temperatures, its immobilization for long-term storage and disposal poses a serious challenge to researchers. Taking this into account, low-temperature stabilization technology based on MKP, a cementitious material, is currently considered promising. We prepared experimental specimens based on Re-incorporated MKP matrices and analyzed their microstructure in detail using analytical methods of X-ray diffraction (XRD), Raman spectroscopy, scanning electron microscopy (SEM), and transmission electron microscopy (TEM). Considering that iron-bearing substances can reduce Tc (VII) to the lower-valence form Tc (IV), which is more stable, attention was also paid to evaluate the effect of fillers (Fe₂O₃, Fe₃O₄, Fe, FeS and blast furnace slag (BFS)) on strength, oxidation state, and water resistance (expressed as leaching cumulative concentration). The addition of fillers ensures the formation of denser compounds based on MKP after 28 days of curing under ambient conditions and increases their mechanical strength. The oxidation state of Re and the reduction from Re (VII) to Re (IV) was estimated using X-ray-absorption near-edge structure (XANES) analysis. Considering the Re leaching concentrations from tests using the ANS-16.1 standard in water, enhanced leachability indices (LI) for Re from MKP matrices were determined with the addition of iron-bearing fillers. Overall, the average LI values were greater than the minimum limit, indicating their acceptance for disposal recommended by the U.S. Nuclear Regulatory Commission. Full article

This paper presents the design, modeling and experimental validation of an on-grid photovoltaic system with self-consumption, sized for the sustainable supply of a water pumping station. The system, composed of 68 photovoltaic panels, uses an architecture based on a Boost DC-DC converter controlled by the Perturb and Observe algorithm, raising the operating voltage to a high-voltage DC bus to maximize the conversion efficiency. The study integrates dynamic performance analysis through simulations in the Simulink environment, testing the stability of the DC bus under sudden irradiance shocks, with rigorous experimental validation based on field production data. The simulation results, which indicate a peak DC power of approximately 34 kW, are confirmed by real monitoring data that records a maximum of 35 kW, the error being justified by the high efficiency of the panels and system losses. Long-term validation, carried out over three years of operation (2023–2025), demonstrates the reliability of the technical solution, with the system generating a total of 124.68 MWh. The analysis of energy flows highlights a degree of self-consumption of 60.08%, while the absence of chemical storage is compensated for by injecting the surplus of 49.78 MWh into the national grid, which is used as an energy buffer. The paper demonstrates that using the grid to balance night-time or meteorological deficits, in combination with a stabilized DC bus, represents an optimal technical-economic solution for critical pumping infrastructures, eliminating the maintenance costs of the accumulators and ensuring continuous operation. Full article

Kidney transplantation remains the ultimate treatment option for patients with end-stage renal disease. However, the global shortage in donor kidneys, exacerbated by challenges such as ischemia–reperfusion injury (IRI), reduces renal graft viability and negatively impacts post-transplant outcomes. Static cold storage, the gold standard of organ preservation, reduces metabolic demand but increases the risk of cold-induced mitochondrial dysfunction and IRI, especially in marginal kidneys. The introduction of machine perfusion techniques allows renal grafts and other solid organ grafts to be preserved at a wider range of temperatures. Organ preservation temperatures play an important role in determining post-transplant outcomes in the transplantation of the kidney and other transplantable solid organs. Therefore, determining the optimal preservation temperature may help increase organ utilization by avoiding unnecessary graft discards and increasing the safe use of marginal organs. This review discusses the impact of various preservation temperatures and methods of preservation on post-transplant outcomes in renal grafts and other organ grafts. Drawing from preclinical, clinical, and meta-analytic studies, we compare hypothermic (0–4 °C), moderate hypothermic (10 °C), subnormothermic (20–32 °C), normothermic (35–37 °C), and subzero preservation strategies, and cellular and molecular changes that occur in renal grafts and other solid organ grafts during preservation at these temperatures. Overall, temperature-controlled machine perfusion outperforms static preservation of renal grafts and other solid organ grafts from marginal and deceased donors, potentially expanding donor pools and improving long-term graft survival, and suggests the need for future research to determine optimal preservation temperature for renal grafts and other solid organ grafts to improve viability and post-transplant outcomes. Full article

Silicone-based implants have been widely used in breast reconstruction but have also been associated with poorly understood complications, including pathologic foreign body responses such as capsular contracture. In this study, we leveraged 3D-printing technology to generate silicone-based implants in a novel, anatomically relevant, prepectoral rat model. We used this model to evaluate the response to an extracellular matrix-based product: ovine-derived reinforced tissue matrix (RTM). Two-piece negative molds were developed through computer-aided design and 3D-printed. The molds were filled with various polydimethylsiloxane mixtures and dip-coated to fabricate implants. Implant material characterization revealed that the implants retained the original 3D-printed mold shape and qualitatively demonstrated a shell with an inner solid gel-like structure. Fabricated implants had smooth surfaces, as well as tunable features including implant stiffness (storage modulus). From initial studies in our rat model, placement of bilateral prepectoral implants allowed assessment of both muscle- and skin-facing capsules and were well-tolerated for at least 12 weeks. Comparison of the foreign body response between RTM-covered and uncovered (control) implants in this model revealed that the capsule thickness did not differ between groups at the 12-week endpoint. However, RTM reduced contractile fibroblasts (alpha-smooth muscle actin) and macrophages (Iba1) compared to the control. Our findings suggested that RTM may improve capsule quality by attenuating cells involved in fibrosis, even when total capsule thickness remains unchanged. However, these changes to cells involved in fibrosis were only observed at this early endpoint and may not predict long-term clinical outcomes. Full article

During the energy storage fracturing process of tight sandstone reservoirs, the pre-injection of fracturing fluid is used to supplement the formation energy, and the physical properties of rocks change under hydration. To reveal the damage mechanism of hydration on tight sandstone, the tight sandstone surrounding the Daqing Changyuan in the northern part of the Songliao Basin was taken as the research object. Through indoor static hydration experiments, combined with scanning electron microscopy (SEM), nuclear magnetic resonance (NMR), Nano-indentation experiments, and other methods, the evolution laws of rock micro-pore morphology, microfracture parameters, Young’s modulus, hardness, and other mechanical indicators under different hydration durations and soaking pressures were systematically explored. The research results show that the water–rock interaction of acidic slick water fracturing fluid significantly changes the mineral composition and microstructure of mudstone and sandstone, controls the development of induced fractures, and degrades the micro-mechanical properties of rocks, with significant lithological differences. In terms of mineral evolution, the soaking time causes the clay minerals in mudstone to increase by up to 12.0%, while pressure causes the carbonate minerals in sandstone to decrease by up to 23.3%. In terms of induced fracture development, the induced fracture widths of sandstone and mudstone under 30 MPa of pressure increase by 122.4% and 85.7%, respectively. The fracture width of mudstone shows a trend of “increasing first and then decreasing” with time, while that of sandstone decreases monotonically. In terms of micro-mechanical properties, after soaking for 168 h, the Young’s modulus of mudstone decreases by up to 66.9%, much higher than that of sandstone (29.5%), while the decrease in hardness of both is similar (58.3% and 59.8%); the mechanical parameters at the induced fractures are only 53.0% to 73.6% of those in the matrix area, confirming the influence of microstructural heterogeneity. This research provides a theoretical basis and data support for optimizing hydraulic fracturing parameters, evaluating wellbore stability, and predicting the long-term development performance in tight sandstone reservoirs. Full article

This study develops an integrated methodological framework coupling CMIP6 climate projections with a socio-economic-hydrological System Dynamics (SD) model to evaluate adaptation strategies for agricultural resilience. Applied to the Qazvin Plain aquifer in Iran, the model demonstrates high fidelity in capturing hydrological–human interactions, evidenced by a 97% correlation between simulated and observed groundwater levels. The system was developed using long-term meteorological drivers (1993–2024) and calibrated against observed socio-hydrological data for the period 2006–2024 and projected to 2062 under multiple CMIP6 scenarios, identifying SSP245 and SSP126 as the most accurate predictors for regional precipitation and temperature, respectively. Modeling outcomes indicate that aridity will intensify across all scenarios; specifically, under current water-use patterns, groundwater storage is projected to decline by 24.5%, 25.4%, and 27.6% by 2041 under SSP126, SSP245, and SSP585, respectively. However, the simulation reveals that integrating demand-side management with crop pattern optimization can stabilize the aquifer and boost agricultural value added by 7.4%. The findings further highlight that a 48% reduction in current groundwater withdrawals is essential to reach a sustainable threshold of 781 million m³. These quantitative insights suggest that while climatic pressures are increasing, human-driven management remains the decisive factor, provided that economic tools and smart monitoring are prioritized for long-term sustainability. Full article

Quantifying e-waste in Sub-Saharan Africa remains constrained by scarce data, weak institutional reporting, and the dominance of informal sector activity. We present the first nationwide assessment of e-waste generation and Random Forest-based national forecasting in Sierra Leone. A mixed-methods survey administered 6000 questionnaires across all 16 districts, targeting households, institutions, enterprises, and informal actors. The study documented devices in use, storage, and disposal across the following six categories: ICT, appliances, lighting, batteries, medical, and other electronics. Population growth and device adoption simulations were combined with lifespan distributions and a Random Forest model trained on survey and simulated historical data to construct e-waste flows and forecast quantities through to 2050, including disposal fate probabilities for repurposing versus discarding. The results showed sharp spatial disparities, with Western Urban (Freetown) averaging about 10 kg per capita compared to 1.8 kg per capita in rural areas. Long-term district patterns were highly concentrated: 50-year annual averages indicated that Western Area Urban contributes 15.3% of national totals, followed by Bo (12.7%) and Western Area Rural (12.1%), with the top five districts contributing 59.1%. By 2050, total national e-waste entering reuse and disposal pathways was projected to reach 23.4 kilo tons per year (kt yr⁻¹) with a 95% uncertainty interval (UI) of 11–42 kt yr⁻¹ (and a 99% interval extending to 50 kt yr⁻¹), corresponding to 0.9–3.4 kg/capita/year. Household appliances dominated total mass, ICT devices exhibited high reuse rates, and batteries showed minimal reuse despite high hazard potential. These findings provide critical evidence for e-waste policy, regulation, and infrastructure planning in data-scarce regions. Full article

Cryopreservation is a critical strategy for the long-term conservation of plant germplasm, particularly for clonally propagated crops, endangered species, and plants producing recalcitrant seeds. Hydrogel-based encapsulation systems can improve survival during ultra-low-temperature storage by providing mechanical protection, moderating dehydration, and regulating cryoprotectant uptake. Although calcium–alginate beads remain the traditional matrix for encapsulation–dehydration and encapsulation–vitrification, recent advances in biomaterials science have enabled the development of composite polysaccharide blends, protein-based matrices, synthetic polymer networks, macroporous cryogels, and functionalized hybrid hydrogels incorporating surfactants, antioxidants, or nanomaterials. These engineered systems provide improved control over water state, pore architecture, diffusion kinetics, and thermal behavior, thereby reducing cryoinjury and enhancing post-thaw recovery across diverse plant explants. This review synthesizes current knowledge on hydrogel platforms used in plant cryopreservation, with emphasis on how physicochemical properties influence dehydration dynamics, cryoprotectant transport, vitrification stability, and rewarming responses. Performance across major explant types is assessed, key limitations in existing materials and protocols are identified, and design principles for next-generation hydrogel systems are outlined. Future progress will depend on material standardization, integration with automated cryopreservation workflows, and the development of responsive hydrogel matrices capable of mitigating cryogenic stresses. Full article

Severe permeability reduction caused by drilling-fluid contamination has significantly impaired injectivity and deliverability in the K gas storage reservoir. This study aims to restore reservoir performance through the optimization and application of a composite acid system. A series of laboratory evaluations combined with core-flow experiments, continuous core scanning, and NMR T₂ analysis were conducted to assess acid performance and elucidate damage-removal mechanisms and pore–throat evolution. The results show that the optimized composite acid exhibits favorable compatibility, effective corrosion and precipitation control, a strong clay-stabilization capacity, and high permeability restoration. Core-scale experiments and NMR analyses indicate that the acid selectively removes near-wellbore and deep plugging while restoring pore–throat connectivity without inducing excessive dissolution or framework damage. Field application further confirms the laboratory findings, demonstrating substantial improvements in gas injection and production performance, along with enhanced reservoir energy retention and recovery. Overall, the proposed composite acid system provides an effective and practical solution for mitigating formation damage and improving the long-term injectivity and deliverability of gas storage reservoirs. Full article

Background/Objectives: Lansoprazole (LNS) is widely used to treat and prevent stomach and intestinal ulcers and as a proton pump inhibitor with low solubility and high permeability. Soluble soybean polysaccharides (SSPS) are well-known disintegrants in food processing but are rarely used in the pharmaceutical field. In this study, we included SSPS as a disintegrant in LNS formulation for pharmaceutical use to investigate the effect of SSPS on the characteristics, dissolution curve, and stability of LNS enteric coated pellets. Methods: The screening of multiple excipients in formulation optimized the release and stability profile of enteric LNS pellets. The final enteric coated LNS pellet containing SSPS were evaluated by LNS crystal form, release profile, and stability. Results: X-ray powder diffraction revealed that this new LNS pellet and commercial reference LNS pellet had similar crystal form by X-ray powder diffraction. Under both long-term and accelerated conditions, these new SSPS-containing LNS pellets had higher release rate and better acid resistance than reference marketed LNS pellets. Conclusions: Inclusion of SSPS in LNS formulation could increase the physicochemical stability of the enteric coated capsules after storage, providing the basis of SSPS for further development and utilization in pharmaceutical formulation as a promising excipient. Full article

Climate change is altering temperature and precipitation regimes in Argentina, with potential consequences for regeneration and persistence of forest tree species, emphasizing the importance of ex situ seed conservation. We evaluated interannual variation in seed traits, desiccation tolerance, storage behavior, and longevity of Cedrela balansae C. DC. and C. fissilis Vell. (Meliaceae), two endangered native species of subtropical rainforests in Argentina. Both species produced desiccation-tolerant seeds, independently of collection year, seed traits, or climatic conditions. Depending on the species, seed traits and longevity varied across years and showed strong relationships with temperature and precipitation, particularly during seed development. Cedrela balansae seeds are medium-lived seeds and have high longevity under standard seed banking conditions, suggesting strong potential for long-term ex situ conservation. Cedrela fissilis seeds are short-lived seeds and have high sensitivity to the storage environment. Correlations among climatic variables and seed traits and longevity parameters suggest that future warming and drying environments may shorten the window for germination and seedling establishment, with species-specific responses depending on climatic conditions during seed development. These results highlight the importance of climate effects in determining seed traits and seed longevity and emphasize the role of seed banking as a critical conservation strategy under climate change. Full article

The storage tank plays a key role in solar thermal installations, as thermal stratification allows high temperatures to be maintained in the upper region while keeping the return temperature to the collectors low. This study analyses the influence of thermal stratification on short- and long-term performance of solar domestic hot water systems using a multi-node storage tank model. An algorithm was developed to compute temperature profiles along the height of a storage tank operating under time-varying temperature and flow-rate conditions. Time courses of temperatures and heat fluxes in a solar domestic hot water system were determined. In addition, the seasonal variation in the optimal locations for supplying the tank with water from the solar collector was identified. Annual simulations were performed for the climate of Kraków (Poland) and the domestic hot water demand of a single-family household. The results show that the effect of the degree of stratification on solar fraction and solar efficiency is small. It was also demonstrated that the effect of thermal stratification within the tank on stabilizing the temperature of the produced water is more significant than the effect associated with increasing the tank volume. Full article