Search Results (7,798)

This study examines the added value of territorial cooperation within the LEADER approach, a key pillar of the EU’s rural development policy. Both interterritorial and transnational cooperation projects empower Local Action Groups (LAGs) to tackle common challenges through innovative and community-driven strategies. Drawing on over 3000 projects since 1994, LEADER cooperation has proven its ability to deliver tangible results—such as joint publications, pilot projects, and shared digital platforms—alongside intangible benefits like knowledge exchange, improved governance, and stronger social capital. By facilitating experiential learning and inter-organizational collaboration, cooperation enables stakeholders to work across territorial boundaries and build networks that respond to both national and transnational development issues. The interaction among diverse actors often fosters innovative responses to local and regional problems. Using a mixed-methods approach, including case studies of Italian LAGs, this research analyses the dynamics, challenges, and impacts of cooperation, with a focus on learning processes, capacity building, and long-term sustainability. Therefore, this study focuses not only on project outcomes but also on the processes and learning dynamics that generate added value through cooperation. The findings highlight how territorial cooperation promotes inclusivity, fosters cross-border dialogue, and supports the development of context-specific solutions, ultimately enhancing rural resilience and innovation. In conclusion, LEADER cooperation contributes to a more effective, participatory, and sustainable model of rural development, offering valuable insights for the broader EU cohesion policy. Full article

Amidst the escalating global challenges of deforestation and climate change, effective forest governance has become a critical global imperative. As a key actor in this arena, China presents a crucial case for understanding state-led environmental governance. This study addresses the thematic evolution and governance structure of China’s forest policy planning from 1980 to 2024. Grounded in multi-level governance (MLG) theory, we apply the Non-negative Matrix Factorization (NMF) topic model to a corpus of 1265 policy documents sourced from the PKULaw database, spanning four administrative levels from central to county. An analysis of 13 core policy themes reveals a significant transition, shifting from early regulatory development and resource utilization to a modern emphasis on ecological protection, scientific monitoring, financial support, and governance innovation. The findings delineate a complex governance architecture: a vertical division of labor (central guidance, local implementation), a horizontal model of inter-departmental interaction where specialized management coexists with comprehensive coordination, and adaptive governance reflecting regional heterogeneity. These results illuminate the dynamic evolution of power allocation, central–local relations, and synergy within China’s forest sector. This study not only provides new empirical evidence and an analytical framework for understanding China’s natural resource policy transition but also offers scientific insights for optimizing multi-level forest governance systems and enhancing policy synergy and efficacy. Full article

Understanding the geographic distribution of mammal species is essential for informed conservation planning, maintaining local ecosystem stability, and addressing research gaps, particularly in data-deficient regions. This study investigated the distribution and richness of 20 mammal species within Nyerere National Park (NNP), a large and understudied protected area in Southern Tanzania. We applied species distribution models (SDMs) using presence data collected through ground surveys between 2022 and 2024, combined with environmental variables derived from remote sensing, including land surface temperature, vegetation indices, soil moisture, elevation, and proximity to water sources and human infrastructure. Models were constructed using the Maximum Entropy (MaxEnt) algorithm, and performance was evaluated using the Area Under the Curve (AUC) metric, yielding high accuracy ranging from 0.81 to 0.97. Temperature (32.3%) and vegetation indices (23.4%) emerged as the most influential predictors of species distributions, followed by elevation (21.7%) and proximity to water (14.5%). Species richness, estimated using a stacked SDM approach, was highest in the northern and riparian zones of the park, identifying potential biodiversity hotspots. This study presents the first fine-scale SDMs for mammal species in Nyerere National Park, offering a valuable ecological baseline to support conservation planning and promote sustainable ecotourism development in Tanzania’s southern protected areas. Full article

Protein association and aggregation are fundamental processes that play critical roles in a variety of biological phenomena from cell signaling to the development of incurable diseases, including amyloidoses. Understanding the basic biophysical principles governing protein aggregation processes is of crucial importance for developing treatment strategies for diseases associated with protein aggregation, including sarcopenia, as well as for the treatment of pathological processes associated with the disruption of functional protein complexes. This work, using a set of methods such as atomic force microscopy (AFM), transmission electron microscopy (TEM), Fourier transform infrared spectroscopy (FTIR), and X-ray diffraction, as well as bioinformatics analysis, investigated the structures of complexes formed by titin and myosin-binding protein C (MyBP-C). TEM revealed the formation of morphologically ordered aggregates in the form of beads during co-incubation of titin and MyBP-C under close-to-physiological conditions (175 mM KCl, pH 7.0). AFM showed the formation of a relatively homogeneous film with local areas of relief change. Fluorimetry with thioflavin T, as well as FTIR spectroscopy, revealed signs of an amyloid-like structure, including a signal in the cross-β region. X-ray diffraction showed the presence of a cross-β structure characteristic of amyloid aggregates. Such structural features were not observed in the control samples of the investigated proteins separately. In sarcomeres, these proteins are associated with each other, and this interaction plays a partial role in the formation of a strong sarcomeric cytoskeleton. We found that under physiological ionic-strength conditions titin and MyBP-C form complexes in which an amyloid-like structure is present. The possible functional significance of amyloid-like aggregation of these proteins in muscle cells in vivo is discussed. Full article

Background: This study aims to evaluate the cost-effectiveness of providing the 23-valent pneumococcal polysaccharide vaccine (PPV23) free of charge versus self-paying vaccination among adults aged 60 years and older in Nanning, Guangxi, China. Methods: A decision tree–Markov model was developed to compare three strategies (government-funded free vaccination, self-funded vaccination, and no vaccination) over a 5-year time horizon. The model incorporated local epidemiological data and cost parameters, applying a 3% discount rate. Sensitivity analyses were conducted on key parameters, including vaccine effectiveness against pneumonia and pneumonia treatment costs. Results: The benefit–cost ratios for free and self-funded vaccination were 0.075 and 0.015, respectively, both below the cost-effectiveness threshold of 1. However, the free vaccination strategy resulted in a higher net benefit (USD 399,651.32) compared to the self-funded strategy (USD 222,594.14), along with a lower Incremental Cost-Effectiveness Ratio (ICER) (USD 1.47 per USD 0.14 of avoided disease cost). Although both strategies yielded benefit–cost ratios far below the conventional threshold of 1, the free strategy demonstrated relatively greater economic efficiency. Sensitivity analyses confirmed that vaccine effectiveness against pneumonia and treatment costs were key drivers of economic outcomes. Conclusions: While neither vaccination strategy achieved conventional cost-effectiveness benchmarks in this setting, the free PPV23 vaccination program demonstrated relatively greater economic efficiency compared to the self-funded approach; although neither strategy met the conventional cost-effectiveness thresholds, they should be considered for inclusion in regional health policy for older adults. Full article

The rapid development of deepfake technologies has led to the widespread proliferation of facial image forgeries, raising significant concerns over identity theft and the spread of misinformation. Although recent dual-domain detection approaches that integrate spatial and frequency features have achieved noticeable progress, they still suffer from limited sensitivity to local forgery regions and inadequate interaction between spatial and frequency information in practical applications. To address these challenges, we propose a novel forgery-aware guided spatial–frequency feature fusion network. A lightweight U-Net is employed to generate pixel-level saliency maps by leveraging structural symmetry and semantic consistency, without relying on ground-truth masks. These maps dynamically guide the fusion of spatial features (from an improved Swin Transformer) and frequency features (via Haar wavelet transforms). Cross-domain attention, channel recalibration, and spatial gating are introduced to enhance feature complementarity and regional discrimination. Extensive experiments conducted on two benchmark face forgery datasets, FaceForensics++ and Celeb-DFv2, show that the proposed method consistently outperforms existing state-of-the-art techniques in terms of detection accuracy and generalization capability. The future work includes improving robustness under compression, incorporating temporal cues, extending to multimodal scenarios, and evaluating model efficiency for real-world deployment. Full article

In response to the escalating global energy demands, the optimization of energy efficiency has emerged as a linchpin for sustainable development. This study considers the potential of energy conservation and emission reduction in one of the most economically vibrant and resource-intensive regions in China, the Yangtze River Economic Belt, encompassing 11 provinces and cities. The SBM-Undesirable model is used to measure the energy efficiency and analyze the temporal-spatial distribution. Moran’s I is employed to analyze the overall spatial pattern and local regional differences in energy efficiency. The systematic analysis shows that the temporal fluctuation exists in the development of energy efficiency, and the average of the Yangtze River Economic Belt exhibits a development pattern of “downstream > midstream > upstream” from the spatial perspective. The upstream region would require way more effort than others to decarbonize and improve efficiency. At the municipal level, the overall energy efficiency of 11 provinces and cities fails to reach an efficient state, and potential for improvement exists. Moreover, the potential model of energy conservation and emission reduction is constructed. We further explore the pathways of energy efficiency improvement for each region in the Yangtze River Economic Belt, including pathways of “High-Efficiency Type”, “High Emission Reduction Potential”, and “Extensive Development Type”. Full article

Against the backdrop of China’s “dual-carbon” initiative, this study innovatively applies a process-based life cycle assessment (PLCA) methodology, meticulously tracking energy and carbon flows across material production, transportation, and maintenance processes. By comparing six asphalt pavement maintenance technologies in Xinjiang, the research reveals that milling and resurfacing (MR) exhibits the highest energy consumption 250,809 MJ/10³ m²) and carbon emissions (15,095.67 kg CO₂/10³ m²), while preventive techniques like hot asphalt grouting reduce emissions by up to 87%. The PLCA approach uncovers a critical insight: 40–60% of total emissions originate from the raw material production phase, with cement and asphalt identified as primary contributors. This granular analysis, unique in regional road maintenance research, challenges traditional assumptions and emphasizes the necessity of upstream intervention. By contrasting reactive and preventive strategies, the study validates that early-stage maintenance aligns seamlessly with circular economy principles. Tailored to a local arid climate and vast transportation network, the study concludes that prioritizing preventive maintenance, adopting low-carbon materials, and optimizing logistics can significantly decarbonize road infrastructure. These region-specific strategies, underpinned by the novel application of PLCA, not only provide actionable guidance for local policymakers but also offer a replicable framework for sustainable road development worldwide, bridging the gap between scientific research and practical decarbonization efforts. Full article

Bovine papillomatosis, caused by a growing group of bovine papillomaviruses (BPVs), is a disease with benign proliferative lesions (papillomas) that may progress to malignancies due to immunological, environmental, or viral factors. This study investigated BPV type diversity in cattle from the Province Santo Domingo de Tsáchilas in Ecuador. Warty lesions were collected from 30 cattle across eight farms. Nucleic acids were extracted using a silicon dioxide-based method, and the partial L1 gene was amplified with PCR. DNA sequences were analyzed using maximum likelihood phylogenetics. Fifty-seven warty lesions yielded ten well-known BPV types: BPV1, BPV2, BPV4, BPV6, BPV8, BPV9, BPV10, BPV13, BPV14, and BPV42. Recently described viral types, BPV-CR2 from Costa Rica and BPV/BR-UEL08 from Brazil, were also detected, alongside a putative novel viral type, BPVEC2024-6-22.1—likely belonging to the genus Xipapillomavirus. This genus had the highest overall count. In contrast, Deltapapillomaviruses were found across all sampled farms. This study underscores BPV diversity in this localized region of Ecuador, and includes genotypes linked to cancers such as enzootic hematuria. The findings provide important epidemiological insights, contributing to vaccine development or immune therapy and improved disease management. Full article

Inhaled corticosteroids (ICSs) are widely used to manage chronic respiratory conditions such as asthma, chronic obstructive pulmonary disease (COPD), and human immunodeficiency virus (HIV). However, prolonged use of ICS is associated with the development of oropharyngeal candidiasis, a fungal infection primarily caused by Candida albicans, due to local immunosuppression in the oral cavity. The incidence of oropharyngeal candidiasis varies depending on geographic region, patient age, and comorbidities, with immunocompromised individuals, those with diabetes, and the elderly being particularly vulnerable. Key risk factors include high ICS doses, poor oral hygiene, and improper use of inhalers. Prevention is the cornerstone of managing oropharyngeal candidiasis associated with the chronic use of inhaled corticosteroids. Patient education on proper inhaler technique and oral hygiene is essential to reduce the risk of fungal overgrowth in the oral cavity. Additional preventive strategies include the use of spacers, mouth rinsing after inhalation, and proper denture care. In cases where these measures fail to prevent the infection, prompt detection and early intervention are crucial to prevent progression or recurrence. This narrative review aims to analyze the most effective prophylactic measures to prevent oropharyngeal candidiasis associated with the chronic use of inhaled corticosteroids, emphasizing patient education, oral hygiene, and proper use of inhalation devices. Full article

The agriculture sector is increasingly challenged to maintain productivity and sustainability amidst environmental, marketplace, and geopolitical pressures. While precision agriculture enhances physical production, the financial resilience of agricultural firms has been understudied. In this study, machine learning (ML) methods, including logistic regression (LR), decision trees (DTs), and artificial neural networks (ANNs), are employed to predict the bankruptcy risk for Central and Eastern European (CEE) farming firms. All models consistently showed high performance, with AUC values exceeding 0.95. DTs had the highest overall accuracy (95.72%) and F1 score (0.9768), LR had the highest recall (0.9923), and ANNs had the highest discrimination power (AUC = 0.960). Visegrad, Balkan, Baltic, and Eastern Europe subregional models featured economic and structural heterogeneity, reflecting the need for local financial risk surveillance. The results support the development of AI-based early warning systems for agricultural finance, enabling smarter decision-making, regional adaptation, and enhanced sustainability in the sector. Full article

The article presents a long-term assessment of the surface water quality of six small rivers in the East Kazakhstan region (Breksa, Tikhaya, Ulba, Glubochanka, Krasnoyarka, and Oba) based on hydrochemical monitoring data from the Kazhydromet State Enterprise for the period 2017–2024. A unified water quality classification system was applied, along with statistical methods, including multiple linear regression. The Glubochanka and Krasnoyarka rivers were identified as the most polluted (reaching classes 4–5), with multiple exceedances of Zn (up to 2.96 mg/dm³), Cd (up to 0.8 mg/dm³), and Cu (up to 0.051 mg/dm³). The most stable and highest water quality was recorded in the Oba River, where from 2021 to 2024, water consistently corresponded to Class 2. Regression models of water quality class as a function of time and annual precipitation were constructed to assess the influence of climatic factors. Statistical analysis revealed no consistent linear correlation between average annual precipitation and water quality (correlation coefficients ranging from −0.49 to +0.37), indicating a complex interplay between climatic and anthropogenic factors. Significant relationships were found for the Breksa (R² = 0.903), Glubochanka (R² = 0.602), and Tikhaya (R² = 0.555) rivers, suggesting an influence of temporal and climatic factors on water quality. In contrast, the Oba (R² = 0.130), Ulba (R² = 0.100), and Krasnoyarka (R² = 0.018) rivers exhibited low coefficients, indicating the predominance of other, likely local, sources of pollution. It was found that summer periods are characterized by the highest pollution due to low water flow, while episodes of acid runoff occur in spring. A decrease in pH below 7.0 was first recorded in 2023–2024 in the Ulba and Tikhaya rivers. Forecasts to 2030 suggest relative stability in water quality under current climatic conditions; however, by 2050, the risk of water quality deterioration is expected to rise due to increased precipitation and extreme weather events. This study presents, for the first time, a systematic long-term analysis of small rivers in the East Kazakhstan region, offering deeper insight into the dynamics of surface water quality and providing a scientific foundation for developing adaptive strategies for the protection and sustainable use of water resources under climate change and anthropogenic pressure. The results emphasize the importance of prioritizing rivers with high variability in water quality for regular monitoring and the development of adaptive conservation measures. The research holds strong applied significance for shaping a sustainable water use strategy in the region. Full article

This study investigates the spatial relationship between population distribution and tree cover loss in Nepal from 2000 to 2020, using satellite-based forest cover and population data along with statistical and geospatial analysis. Two statistical methods—linear regression (LR) and Geographically Weighted Regression (GWR)—were used to assess the influence of population on forest cover change. The correlation between total population and forest loss at the national level suggested little to no direct impact of population growth on forest loss. However, sub-national analysis revealed localized forest degradation, highlighting the importance of spatial and regional assessments to uncover land cover changes masked by national trends. While LR showed a weak national-level correlation, GWR revealed substantial spatial variation, with the coefficient of determination values increasing from 0.21 in 2000 to 0.59 in 2020. In some regions, local R² exceeded 0.75 during 2015 and 2020, highlighting emerging hotspot clusters where population pressure is strongly linked to deforestation, especially along major infrastructure corridors. Using very high-resolution spatial data enabled pixel-level analysis, capturing fine-scale deforestation patterns, and confirming hotspot accuracy. Overall, the findings emphasize the value of spatially explicit models like GWR for understanding human–environment interactions guiding targeted land use planning to balance development with environmental sustainability in Nepal. Full article

The lack of new materials with desired processability and functional characteristics remains a challenge for metal additive manufacturing (AM). Therefore, in this work, a new promising AISI 316L-based alloy with better performance compared to the commercially available one is developed via the laser powder bed fusion (L-PBF) process. Moreover, establishing process–structure–properties linkages is a critical point that should be evaluated carefully before adding newly developed alloys into the AM market. Hence, the current study investigates the influences of various process parameters on the as-built quality and microstructure of the newly developed alloy. The results revealed that increasing laser energy density led to reduced porosity and surface roughness, likely due to enhanced melting and solidification. Microstructural analysis revealed a uniform distribution of copper within the austenite phase without forming any agglomeration or secondary phases. Electron backscatter diffraction analysis indicated a strong texture along the build direction with a gradual increase in Goss texture at higher energy densities. Grain boundary regions exhibited higher local misorientation and dislocation density. These findings suggest that changing the process parameters of the L-PBF process is a promising method for developing tailored microstructures and chemical compositions of commercially available AISI 316L stainless steel. Full article

Despite the well-documented health risks of noise pollution, its impact remains overlooked mainly in life cycle assessment (LCA). This study introduces a methodological innovation by integrating both traffic and construction noise into the LCA framework for concrete construction, providing a more holistic and realistic evaluation of environmental and health impacts. By combining building information modeling (BIM) with LCA, the method automates material quantification and assesses both environmental and noise-related health burdens. A key advancement is the inclusion of health-based indicators, such as annoyance and sleep disturbance, quantified through disability-adjusted life years (DALYs). Two scenarios are examined: (1) a comparative analysis of concrete versus timber flooring and (2) end-of-life options (reuse vs. landfill). The results reveal that concrete has up to 7.4 times greater environmental impact than timber, except in land use. When noise is included, its contribution ranges from 7–33% in low-density regions (Darwin) and 62–92% in high-density areas (NSW), underscoring the critical role of local context. Traffic noise emerged as the dominant source, while equipment-related noise was minimal (0.3–1.5% of total DALYs). Timber slightly reduced annoyance but showed similar sleep disturbance levels. Material reuse reduced midpoint environmental impacts by 67–99.78%. Sensitivity analysis confirmed that mitigation measures like double glazing can cut noise-related impacts by 2–10% in low-density settings and 31–45% in high-density settings, validating the robustness of this framework. Overall, this study establishes a foundation for integrating noise into LCA, supporting sustainable material choices, environmentally responsible construction, and health-centered policymaking, particularly in noise-sensitive urban development. Full article