Search Results (11,119)

The exponential increase in photovoltaic panel (PV) waste highlights the urgent need to develop efficient and sustainable recycling processes. It is estimated that by 2030, 8 million tons of PV modules will reach their end-of-life stage, posing a significant environmental challenge and requiring the development of green technologies for resource recovery. This study assessed the performance of imidazolium-based ionic liquids (ILs) as “designer solvents” for the selective leaching of copper and silver from disused PV panels. Specifically, four quaternary imidazolium salts were evaluated: [Bmim]HSO₄, [Emim]HSO₄, [Bmim]Cl, and [Emim]Cl. Leaching tests were conducted on silicon wafers containing 0.28% Ag and 0.19% Cu under varying temperatures (25, 50, and 80 °C), IL concentrations (20% and 60% v/v), and hydrogen peroxide (H₂O₂) dosages (0% and 3% v/v) as an oxidizing agent. The results identified [Bmim]HSO₄ as the most effective leaching agent. The system achieved a maximum copper extraction of 96.70% at 60% v/v concentration and 80 °C. For silver, the highest extraction of 45.13% was obtained using [Bmim]HSO₄ at 20% v/v and 80 °C. The addition of H₂O₂ was crucial, demonstrating a clear synergistic effect with the imidazolium-based ILs by promoting oxidative dissolution. These findings confirm that imidazolium-based ionic liquids represent a promising and environmentally friendly alternative for the recovery of high-value metals in the circular economy of photovoltaic recycling. Full article

The safety and stability of onboard Liquid Hydrogen (LH₂) storage systems depend strongly on gas–liquid two-phase flow, heat transfer, and phase change under sloshing; however, the coupled influence of filling ratio and sloshing on thermo-hydrodynamic behavior remains underexplored. We develop a Volume of Fluid (VOF)-based two-phase Computational Fluid Dynamics (CFD) model in ANSYS Fluent to quantify interfacial dynamics, pressure response, and temperature-field evolution in LH₂ tanks subjected to sinusoidal acceleration for filling ratios from 10% to 90%. Increasing the filling ratio strengthens net condensation in the ullage and thus intensifies depressurization. As the filling ratio increases from 10% to 90%, the pressure reduction over the 2.0 s sloshing process increases from 0.418 kPa to 2.410 kPa, and the corresponding initial depressurization rate rises from 0.209 to 1.205 kPa s⁻¹. Free-surface motion decreases with filling ratio: at 10%, large interface excursions can induce gas-cavity formation and splashing, increasing the risk of intermittent propellant supply, whereas at 90% the interface is constrained and oscillations are suppressed. Higher filling ratios lead to faster ullage cooling and larger temperature oscillations. The liquid warms modestly, and its warming rate decreases nonlinearly with filling ratio, consistent with the larger effective thermal mass at higher fillings. Overall, the obtained mechanistic understanding can support the engineering design of onboard LH₂ tanks, including filling-ratio selection and thermal-management optimization under sloshing conditions. Full article

Venous thromboembolism (VTE) significantly impacts lung adenocarcinoma outcomes, yet current predictive tools lack precision. We investigated plasma extracellular vesicle (EV) mRNA as a liquid biopsy source to identify a pro-thrombotic molecular profile in VTE patients. Within a prospective cohort of 260 patients, we performed a retrospective nested case–control study, matching 10 VTE cases with 11 thrombosis-free controls. Plasma EV-RNA was analyzed via high-throughput sequencing. Differentially expressed genes (DEGs) were integrated with functional enrichment and explored across public non-cancer VTE datasets, buffy coat samples, and cell lines. RNA-seq identified 483 DEGs within the VTE patient EV compartment, predominantly linked to neutrophil degranulation (NETosis), inflammation, and coagulation. We identified a set of EV-associated candidate genes (SELP, ELANE, MYL9, DNASE1L3) distinguishing cancer-associated thrombosis from non-malignant VTE, along with transcripts (TFPI, FCGR2A) selectively enriched within the EV compartment relative to circulating blood cells. P-selectin (SELP) was the only significantly increased marker, providing the strongest complementary support at the protein level. This molecular state was detectable prior to the occurrence of VTE. Plasma EVs capture a multicellular mRNA profile, reflecting the systemic immunothrombotic activation in lung adenocarcinoma. Despite sample size limitations, these findings should be considered exploratory and hypothesis-generating, but they suggest the EV-derived mRNA in combination with circulating markers such as SELP may provide a framework for future studies aimed at improving risk stratification. Full article

Rising demand for high-performance battery thermal management systems (BTMSs) has rendered single-mode cooling insufficient for advanced lithium-ion batteries (LIBs) in new energy vehicles (NEVs), particularly under high discharge rates. This study proposes a synergistic hybrid BTMS integrating composite phase-change material (CPCM)–aluminum foam with liquid cooling to enhance thermal regulation of cylindrical battery modules under 5 C discharge conditions. Multiple liquid-cooled plate (LCP) configurations, including serpentine, straight, and leaf-shaped designs, together with different flow channel topologies (FCTs), were systematically investigated and optimized. The effects of coolant flow speed (CFS) and ambient temperature were also analyzed. Results indicate that the optimized leaf-shaped LCP with FCT #2 delivers superior performance, limiting the maximum temperature to 309.98 K, reducing temperature difference by 7.6%, and decreasing pressure drop by 88.79% compared to the serpentine configuration. Increasing CFS improves heat dissipation and delays PCM melting, although it raises pressure losses. Furthermore, the proposed system maintains a cell-to-cell temperature difference below 0.51 K, indicating excellent thermal uniformity. Compared to a CPCM-only system, the hybrid BTMS reduces peak temperature by 8.81 K under elevated ambient conditions (309.15 K), demonstrating strong potential for reliable and efficient thermal management in demanding operating environments. Full article

Background: The growing complexity of liquid biopsy in precision oncology demands a structured classification framework that can accommodate its expanding multi-omic scope. As the field has matured from early Tumor Microemboli research—focused on multicellular clusters of circulating tumor cells (CTCs) that drive high-efficiency metastasis—to the broader systemic analysis of the “Tumor Microenvironment” (TME) encompassing malignant and non-malignant components, the need for a hierarchical taxonomy has become evident. Objective: To integrate these diverse data streams into a coherent clinical framework, a multi-tiered classification system is needed. This review proposes a foundational roadmap that formally distinguishes the systemic ecosystem from its physical and functional subsets and highlights their clinical utility in therapeutic decision-making. Proposed Taxonomy: We advocate for the adoption of Circulating Tumor Microenvironment (cTME) as the inclusive term for the systemic environment, encompassing non-cellular factors such as ctDNA, extracellular vesicles, and biophysical attributes. Conversely, physical cellular clusters should be strictly classified as Circulating Tumor Emboli (CTE). Crucially, we define Circulating Tumor-Associated Cells (C-TACs) as the functional cellular subset within the cTME, encompassing single CTCs, CTE, and supporting non-malignant cells like CTECs and CAFs. Clinical Applications: Establishing this distinction allows for the seamless integration of molecular profiling (NGS) and functional assays. We highlight emerging evidence that C-TACs may serve as the primary substrate for Chemo-Response Profiling (CRP), with early proof-of-concept studies reporting high concordance with clinical outcomes that still await independent prospective confirmation. Furthermore, preliminary evidence suggests that identifying these functional units, particularly perioperative CTE, may help predict the efficacy of adjuvant chemotherapy in early-stage malignancies, although this remains to be confirmed in prospective studies. Conclusions: Adopting this unified taxonomy may help advance precision oncology. By recognizing the cTME as the superordinate ecosystem and C-TACs as its functional executors, clinicians may be better positioned to interpret multi-modal liquid biopsy data, providing a conceptual roadmap for integrating these technologies into platforms for personalized cancer management. We emphasize that this framework is intended to be hypothesis-generating and that its clinical applications require prospective validation before routine adoption. Full article

Background/Objectives: Immune checkpoints are critical regulatory pathways that maintain peripheral tolerance and prevent autoimmunity. Among these, the programmed death-1/programmed death-ligand 1 (PD-1/PD-L1) axis serves as a major inhibitory pathway that terminates T cell responses. While protein-based checkpoint blockade (ICB) targeting this axis has revolutionized clinical cancer therapy, its clinical efficacy is frequently limited by low response rates, immune-related adverse events (irAEs), and the emergence of adaptive resistance. To break through these bottlenecks, genetic interruption has emerged as a high-precision alternative to modulate the PD-1/PD-L1 pathway at the nucleotide level. Methods: A comprehensive systematic review of literature was performed across major databases (PubMed, Web of Science), with a focus on high quality studies published up to 2026. Results: Direct genomic disruption via CRISPR/Cas9 and post-transcriptional silencing through RNA interference can effectively neutralize inhibitory signaling at its source. Recent advances demonstrate that targeting upstream regulatory nodes—including metabolic checkpoints (e.g., lactate metabolism) and biophysical mechanisms (e.g., liquid–liquid phase separation)—provides superior transcriptional control over PD-L1. Furthermore, engineering CAR-T cells with multiplex gene editing (e.g., TCR/B2M/PD-1 knockout) or localized scFv secretion significantly enhances antitumor potency while reducing systemic toxicity. Innovations in organ-targeted lipid nanoparticles and stimuli-responsive biomimetic carriers further address the delivery barriers in solid tumors. Conclusions: Gene therapy provides a high-precision platform for PD-1/PD-L1 modulation, offering a viable strategy to overcome adaptive resistance. Future clinical application depends on the refinement of safer editing tools, such as base editing, and the standardization of intelligent delivery systems to ensure controllable and scalable cancer immunotherapy. Full article

Rapid detection of foodborne pathogenic and spoilage microorganisms is critical for ensuring food safety and quality in liquid matrices. While Raman tweezers spectroscopy (RTS) enables label-free single-cell analysis, its application in high-throughput inline inspection faces a fundamental bottleneck: high flow rates required for efficiency induce severe motion blur and low signal-to-noise ratios (SNR), which blind automated control systems and destabilize optical trapping. To overcome this, we present a Spatiotemporal Video-Enhanced Raman Tweezers (SVERT) system integrating a deceleration-optimized microfluidic chip with a deep learning-based visual feedback loop. We propose a Local–Global Unified Denoising Network (LGU-Net) tailored to recover high-fidelity bacterial structures from low-SNR video streams, achieving a deterministic processing latency of ~0.49 ms. Experimental results demonstrate that SVERT improves the optical trapping success rate from 21.27% ± 2% to 91.47% ± 1.8% compared to raw video input, enabling a four-fold increase in spectral acquisition efficiency. Leveraging the acquired high-quality dataset, we achieved a classification accuracy of 96.74% across four bacterial species of relevance to food safety and quality. Crucially, we validated the system’s practical robustness by successfully isolating and tracking trace E. coli in an unpurified commercial beverage. This capability to effectively mitigate natural background interference demonstrates the system’s promising potential to be expanded for broader applications in liquid food safety screening. Full article

A surface tension pump, a type of passive pumping method, can generate a gentle and low-flow liquid transport in microchannels without external equipment or tubing, even under microgravity conditions. However, its applicability is limited for long-term operation with large liquid volumes due to its reliance on phenomena specific to small liquid volumes. To overcome this limitation, we developed an automatic reagent dispensing system enabling intermittent replenishment of the inlet reservoir in microfluidic devices. The system achieved high positional repeatability, with a maximum error below 781 µm, which was sufficient for operation within the inlet well used in this study. Initial flow-rate characterization demonstrated that the flow behavior could be adjusted through the dispensed droplet volume. The system was further evaluated through an 18 h automated cell-culture experiment, showing cell-retention performance comparable to that obtained by manual medium replenishment. These results demonstrate the feasibility of using automated intermittent replenishment to extend the operating duration of passive pumping systems. Full article

Solar-driven interfacial evaporation (SIE) has emerged as a transformative, off-grid technology that confines heat at the air–liquid interface, enabling high-efficiency vapor generation for decentralized water purification. Here, we present a comprehensive and critical review of the field, charting its evolution from fundamental photothermal principles to integrated multifunctional systems. We first elucidate the thermodynamics of interfacial heat localization and the resultant enhancement in evaporation efficiency. We then systematically analyze material innovation strategies—including broadband-absorbing photothermal agents and tailored evaporator architectures—designed to overcome persistent challenges such as salt crystallization, fouling, and thermal losses. Moving beyond freshwater production, we highlight emerging pathways for extending SIE platforms toward water–energy cogeneration, selective resource recovery, and zero-liquid-discharge wastewater treatment. We further identify and objectively assess the key bottlenecks that currently hinder the transition from laboratory-scale prototypes to real-world deployment, with a focus on long-term material robustness under harsh environments, adaptability to fluctuating water chemistries, and techno-economic viability. Finally, we outline forward-looking research directions, including stimulus-responsive smart evaporators, elucidation of multi-field coupling mechanisms, and the establishment of standardized performance evaluation protocols. This review aims to provide both a tutorial for newcomers and a critical assessment for experienced researchers, offering a balanced perspective on the current state-of-the-art and a roadmap for translating SIE from academic research into sustainable, impactful technologies. Full article

Liquid storage tanks are widely used in sectors such as water treatment, oil and gas, food processing, and chemical manufacturing. Knowing the exact amount of liquid in a tank is essential for ensuring safety, preventing spills, and optimizing process control; therefore, the liquid level in a tank must be maintained at a precise reference point. This is where liquid level control for tanks becomes crucial and constitutes a fundamental problem in the industrial sector due to nonlinearities, multivariable coupling, and stochastic disturbances. Given the drawbacks of available control methods, such as classical Model Predictive Control (MPC), which are highly dependent on model accuracy and struggle to reject complex stochastic noise, predicting random disturbances represents a major technological challenge. A new approach is proposed to specifically address the problem and challenge of the four-tank system, where water levels in two lower tanks must be controlled by two pumps, often with varying delays and significant parameter disturbances. To establish a relationship between expected performance and MPC parameters, this approach uses a novel hybrid nonlinear MPC, Extended State Observer, and Physics-Informed Neural State Estimation (NMPC-ESO-PINSE) architecture. A Physics-Informed Neural State Estimation (PINSE) layer, chosen for its learning capacity, is designed to filter sensor noise by applying Bernoulli’s physical laws, while an Extended State Observer (ESO) is integrated to capture and compensate for unmodeled uncertainties in the process. Finally, a proposed hybrid (NMPC-ESO-PINSE) strategy leverages these clean, physically consistent state estimations to solve a non-convex optimization problem via Sequential Quadratic Programming (SQP), computing optimal pump voltages. Extensive numerical simulations demonstrate the superior resilience of this decoupled framework against parametric drifts and continuous noise sequences, yielding a +27.36% reduction in global Root Mean Square Error (RMSE) compared to standard NMPC, accelerating the closed-loop settling time to 15.2 s, and restricting transient overshoot to just 0.18%. Full article

A silk fibroin/cellulose inverse-opal photonic crystal composite with robust mechanical properties was fabricated by blending a silk fibroin solution with methylcellulose, utilizing a 3D poly(methyl methacrylate) (PMMA) photonic crystal array as a template, via sequential infiltration, curing, and etching processes. Leveraging the intrinsic water sensitivity of both silk fibroin and methylcellulose, the resulting composite exhibits exceptional moisture-sensing capabilities across gaseous, liquid, and solid phases. Specifically, for atmospheric humidity, the film delivers a distinct optical response to a relative humidity variation in merely 5%. In liquid systems, owing to the material’s excellent affinity for low-polarity organic solvents and the disruptive effect of highly polar solvents (e.g., water) on the photonic periodic structure, the structural color of the film can sensitively report trace water contents down to 0.025%. Furthermore, in solid matrices, the composite enables the precise detection of not only free water but also water of crystallization. Full article

Cystic fibrosis (CF) is a genetic disorder caused by dysfunction of the CFTR chloride ion channel. Progress in molecular understanding and therapy development relies on advanced cellular models and robust assays for evaluating CFTR function. This review traces the evolution of in vitro models, from primary and immortalized cell lines to patient-specific induced pluripotent stem cells (iPSCs) and complex three-dimensional systems. These advanced models, including air-liquid interface (ALI) cultures, organoids, and microfluidic organ-on-a-chip platforms, enable recapitulation of tissue architecture, cellular heterogeneity, and key pathological features such as impaired mucociliary clearance and chronic inflammation. A critical component of CF research is the accurate functional assessment of CFTR activity. We compare established high-resolution techniques (patch-clamp, Ussing chamber) with high-throughput screening assays, including fluorescence quenching of halide-sensitive YFP assay and organoid swelling tests. The article provides a framework for choosing the most appropriate CFTR functional assay tailored to specific research goals. Full article

To reveal the nonlinear instability mechanism by which the three-degree-of-freedom rotor system of a magnetic-liquid double suspension bearing transforms from stable suspension to periodic vibration, a nonlinear dynamic model considering electromagnetic suspension force, hydrostatic supporting force, rotor unbalance force, and liquid film resistance is established. The equilibrium point of the system is linearized, and the Hopf bifurcation boundary is determined using the Routh–Hurwitz criterion. Numerical simulations are then carried out to investigate the effects of the initial current i₀, supply flow rate q₀, and different initial disturbances on the displacement time histories, phase trajectories, and spatial phase trajectories of the rotor. The results show that, under the given system parameters, the Hopf bifurcation boundary is 0.61 A for the initial current and 9.62 × 10⁻⁵ m³/s for the supply flow rate. Current variation mainly affects electromagnetic stiffness and nonlinear electromagnetic force, whereas flow rate variation primarily changes the hydrostatic load capacity and oil film damping characteristics. Under different initial disturbances, the system may exhibit amplitude attenuation, recovery to stable suspension, or finite amplitude periodic vibration. Experimental results show good agreement with numerical simulations in terms of frequency spectra, displacement time histories, and phase trajectories, thereby verifying the effectiveness of the proposed three-degree-of-freedom dynamic model and Hopf bifurcation analysis method. The results can provide theoretical guidance for parameter matching, stability evaluation, and self-excited vibration suppression of magnetic-liquid double suspension bearings. Full article

The vitreous body is not only a transparent filling material of the posterior segment; it is a soft, hydrated, and biologically active matrix that supports structural, optical, and biochemical homeostasis. Vitrectomy therefore leaves a functional deficit that current substitutes only partly address. Intraocular gases, silicone oils, and perfluorocarbon liquids remain essential surgical tools, but they mainly provide mechanical tamponade and do not reproduce native viscoelasticity, diffusion control, or protection against oxidative and inflammatory stress. This review considers vitreous replacement as a functional biomaterials challenge. We discuss native vitreous physiology, the limitations of present tamponade agents, and emerging bioengineered substitutes designed to create a more physiological intravitreal environment. Particular attention is given to hydrogel and polymer-based systems, especially hyaluronic acid-based and in situ crosslinked platforms, which are being developed to combine optical clarity, injectability, soft mechanical support, controlled degradation, and favorable tissue interaction. We also emphasize the need for standardized preclinical testing of swelling, enzymatic stability, drug diffusion, rheology, and long-term biocompatibility. Although next-generation materials may move the field beyond passive space filling, manufacturing reproducibility, regulatory validation, chronic safety, and cautious early-phase trials remain major translational barriers. Full article

Ionic liquid-based localized high-concentration electrolytes, leveraging their intrinsically nonflammable safety characteristics and wide electrochemical windows, have emerged as strong contenders for next-generation lithium metal battery electrolytes. However, because such systems are anion-rich, the electrolyte bulk phase tends to form solvation structures dominated by bulky anionic clusters along with an excess of free anions, which triggers persistent and uncontrollable anion decomposition at the interphase. To address this issue, we adopt a strategy of constructing a compressed solvation structure by introducing a weakly interacting chlorinated diluent (TeCA), which helps form a compact solvation environment and alleviates excessive anion decomposition at electrode interphases. In this work, 1,1,2,2-tetrachloroethyl acetate (TeCA) was introduced as a weakly coordinating chlorinated diluent into an ionic-liquid localized high-concentration electrolyte (LHCE) to regulate the Li⁺-FSI⁻ solvation environment. By combining Raman spectroscopy, molecular dynamics simulations, and electrochemical characterization, the TeCA-LHCE system was found to exhibit altered ion-cluster configurations, improved oxidation tolerance, and enhanced interfacial stability under high-voltage conditions. The as-prepared TeCA-LHCE electrolyte presents improved electrochemical performance in comparison with TTE-LHCE and the baseline electrolyte (BE). The Li||Cu half-cell employing TeCA-LHCE achieved a high Coulombic efficiency above 99% over 500 cycles and formed a uniform and dense lithium deposition layer without obvious dendritic growth. When paired with a high-loading NCM811 cathode (10 mg cm⁻²), the TeCA-LHCE-based Li||NCM811 full cell delivered significantly improved cycling stability and rate capability under a high cutoff voltage of 4.3 V. Full article