Search Results (339)

Ultraviolet B (UVB) radiation is a key factor in the overproduction of melanin in the skin. Melanocytes produce melanin through melanogenesis to protect the skin from UVB radiation-induced damage. However, excessive melanogenesis can lead to hyperpigmentation and increase the risk of malignant melanoma. Tyrosinase is the rate-limiting enzyme in melanogenesis; it catalyzes the oxidation of tyrosine to 3,4-dihydroxy-L-phenylalanine and subsequently to dopaquinone. Thus, inhibiting tyrosinase is a promising strategy for preventing melanogenesis and skin hyperpigmentation. White mulberry (Morus alba L.) is rich in antioxidants, and mulberry fruit extracts have been used as cosmetic skin-lightening agents. However, data on the capacity of mulberry fruit extracts to inhibit tyrosinase under UVB radiation-induced melanogenic conditions remain scarce, especially in an in vivo model. In this study, we evaluated the effects of a mulberry crude extract (MCE) on UVB radiation-induced melanogenesis in B16F10 melanoma cells and zebrafish embryos. The MCE significantly reduced tyrosinase activity and melanogenesis in a dose-dependent manner without inducing cytotoxicity. These effects are likely attributable to the antioxidant constituents of the extract. Our findings highlight the potential of this MCE as an effective tyrosinase inhibitor for the prevention of UVB radiation-induced skin hyperpigmentation. Full article

Considering the importance of the automotive industry, reducing the environmental impact of automotive component manufacturing is crucial. Additionally, lightening of the latter promotes a reduction in fuel consumption throughout the vehicle’s life cycle, limiting emissions. This study presents a comprehensive approach to optimizing and manufacturing a MacPherson steering knuckle using topology optimization (TO), additive manufacturing, and rapid investment casting (RIC). Static structural simulations confirmed the mechanical integrity of the optimized design, with stress and strain values remaining within the elastic limits of the SG A536 iron alloy. The TO process achieved a 30% reduction in mass, resulting in lower material use and production costs. Additive manufacturing of optimized geometry reduced resin consumption by 27% and printing time by 9%. RIC simulations validated efficient mold filling and solidification, with porosity confined to removable riser regions. Life cycle assessment (LCA) demonstrated a 27% reduction in manufacturing environmental impact and a 31% decrease throughout the component life cycle, largely due to vehicle lightweighting. The findings highlight the potential of integrated TO and advanced manufacturing techniques to produce structurally efficient and environmentally sustainable automotive components. This workflow offers promising implications for broader industrial applications that aim to balance mechanical performance with ecological responsibility. Full article

In the context of efforts to reduce greenhouse gas emissions in the building sector, the reintegration of traditional earthen construction into modern architectural and renovation practices offers a sustainable alternative. To address the mechanical and water-resistance limitations of adobe bricks, the use of agricultural waste—such as rice husk—is increasingly being explored. This experimental study evaluates the effects of rice husk addition on the mechanical, hygrothermal, and acoustic properties of adobe bricks. Two soil types—one siliceous and one calcareous—were combined with 1, 2, and 3 wt% rice husk to produce bio-based earthen bricks. The influence of rice husk was found to depend strongly on the soils’ mineralogical and granulometric characteristics. The most significant improvements were in hygrothermal performance: at 3 wt%, thermal conductivity was reduced by up to 35% for calcareous soil and 20% for siliceous soil, indicating enhanced insulation. Specific heat capacity also increased with husk content, suggesting better thermal inertia. The moisture buffering capacity, already high in raw soils, is further improved due to increased surface porosity. Mechanically, rice husk incorporation had mixed effects: a modest increase in compressive strength was observed in siliceous soil at 1 wt%, while calcareous soil showed slight improvement at 3 wt%. Acoustic performance remained low across all samples, with minimal gains attributed to limited macro-porosity. These findings highlight the importance of soil composition in optimizing rice husk dosage and suggest promising potential for rice husk-stabilized adobe bricks, especially in thermally demanding environments. Full article

Theobroma cacao L. (cocoa) pod husk, a byproduct of the chocolate industry, has potential for commercial applications due to its bioactive compounds. This study aimed to determine the phytochemical composition, biological activity, and clinical efficacy of a standardized extract. This study compared 80% ethanol (CE) and 80% ethanol acidified (CEA) as extraction solvents. The result indicated that CEA yielded higher total phenolic content (170.98 ± 7.41 mg GAE/g extract) and total flavonoid content (3.91 ± 0.27 mg QE/g extract) than CE. Liquid chromatography–tandem mass spectrometry (LC/MS/MS) identified various phenolic and flavonoid compounds. CEA demonstrated stronger anti-oxidant (IC₅₀ = 5.83 ± 0.11 μg/mL in the DPPH assay and 234.17 ± 4.01 mg AAE/g extract in the FRAP assay) compared to CE. Additionally, CEA exhibited anti-tyrosinase (IC₅₀ = 9.51 ± 0.01 mg/mL), anti-glycation (IC₅₀ = 62.32 ± 0.18 µg/mL), and anti-collagenase (IC₅₀ = 0.43 ± 0.01 mg/mL), nitric oxide (NO) production inhibitory (IC₅₀ = 62.68 μg/mL) activities, without causing toxicity to cells. A formulated lotion containing CEA (0.01–1.0% w/w) demonstrated stability over six heating–cooling cycles. A clinical study with 30 volunteers showed no skin irritation. The 1.0% w/w formulation (F4) improved skin hydration (+52.48%), reduced transepidermal water loss (−7.73%), and decreased melanin index (−9.10%) after 4 weeks of application. These findings suggest cocoa pod husk extract as a promising active ingredient for skin hydrating and lightening formulation. Nevertheless, further long-term studies are necessary to evaluate its efficacy in anti-aging treatments. Full article

As streaming platforms adopt artificial intelligence (AI)-powered subtitle systems to satisfy global demand for instant localization, the cognitive impact of these automated translations on viewers remains largely unexplored. This study used a web-based eye-tracking protocol to compare the cognitive load that GPT-4o-generated Arabic subtitles impose with that of professional human translations among 82 native Arabic speakers who viewed a 10 min episode (“Syria”) from the BBC comedy drama series State of the Union. Participants were randomly assigned to view the same episode with either professionally produced Arabic subtitles (Amazon Prime’s human translations) or machine-generated GPT-4o Arabic subtitles. In a between-subjects design, with English proficiency entered as a moderator, we collected fixation count, mean fixation duration, gaze distribution, and attention concentration (K-coefficient) as indices of cognitive processing. GPT-4o subtitles raised cognitive load on every metric; viewers produced 48% more fixations in the subtitle area, recorded 56% longer fixation durations, and spent 81.5% more time reading the automated subtitles than the professional subtitles. The subtitle area K-coefficient tripled (0.10 to 0.30), a shift from ambient scanning to focal processing. Viewers with advanced English proficiency showed the largest disruptions, which indicates that higher linguistic competence increases sensitivity to subtle translation shortcomings. These results challenge claims that large language models (LLMs) lighten viewer burden; despite fluent surface quality, GPT-4o subtitles demand far more cognitive resources than expert human subtitles and therefore reinforce the need for human oversight in audiovisual translation (AVT) and media accessibility. Full article

Facial aging is a complex process manifesting as skin hyperpigmentation, textural irregularities, and a diminished elasticity, hydration, and evenness of tone. The escalating demand for minimally invasive aesthetic interventions has driven the development of advanced hydrogel-based injectable formulations. This clinical study assessed the safety and efficacy of Hydragel A1, an injectable hydrogel containing hyaluronic acid (HA), niacinamide, and tranexamic acid (TXA), designed to simultaneously address multiple facets of facial skin aging. A cohort of 49 female participants underwent a series of objective and subjective assessments, including the Global Aesthetic Improvement Scale (GAIS), instrumental measurements (Antera 3D, Chromameter, Cutometer, Dermascan, Corneometer), and standardized photographic documentation at baseline (Day 0) and 14, 28, and 70 days post-treatment. The results demonstrated statistically significant improvements in skin hydration, texture, elasticity, and pigmentation following Hydragel A1 administration. Notably, no serious adverse events or significant injection site reactions were observed, confirming the favorable safety profile of the investigated device. Collectively, these findings underscore the potential of a combined HA, niacinamide, and TXA injectable formulation to provide a comprehensive approach to facial skin rejuvenation, effectively targeting multiple aging-related mechanisms. Full article

During processing and storage, vegetables often experience undesirable color changes, including fading, lightening, or yellowing and softening, due to browning (enzymatic and non-enzymatic) and chlorophyll degradation. These changes diminish commercial and nutritional value. Therefore, it is necessary to maintain vegetable color and improve the quality of vegetable-based dishes. To address these issues, it is a scientific and practical necessity to summarize and discuss existing strategies and innovative techniques. This review first highlights the mechanisms of vegetable browning. This review then provides a comprehensive overview of recent advances in methods for color preservation, focusing on underlying mechanisms and techniques for inhibiting color changes from physical, chemical, and biological perspectives. A review of innovative technologies suggests that effective color preservation in vegetables is achieved by inhibiting the conditions that lead to three unfavorable color change reactions: enzymatic browning, non-enzymatic browning, and chlorophyll degradation. Current research frequently employs combined approaches that integrate two or more techniques to mitigate these adverse color changes. Moreover, most of these methods could simultaneously inhibit the three reaction processes. Future research directions are proposed for in-depth investigations into the molecular mechanisms of color changes in vegetables and the impact of treatments on the nutritional value. Full article

The paper is devoted to the plasticization mechanisms of alkali-activated cement system Na₂O-CaO-Al₂O₃-SiO₂-H₂O. The fundamentals and basic factors determining the effectiveness of plasticizing surfactants for alkali-activated cement materials are discussed. The factors under consideration in the study were alkali-activated cement basicity (the content of granulated blast furnace slag), the anion of the alkaline component or activator, and the degree of dispersing of the cement particles in the system. The action effect of plasticizers was determined by finding the interrelation between the stability of its molecular structure, degree of adsorption, and molecular weight depending on mentioned basic factors. A systematic approach to the systematization of surfactants and their choice to be taken into consideration to control technology-related and physico-mechanical properties of alkali-activated cement-based heavyweight concretes, building mortars, and lightened grouts has been proposed. Full article

Bayesian networks (BNs) are popular models that represent complex relationships among variables. In the discrete case, these relationships can be quantified by conditional probability tables (CPTs). CPTs can be derived from data, but if data are not sufficient, experts can be involved to assess the probabilities in the CPTs through Structured Expert Judgment (SEJ). This is often a burdensome task due to the large number of probabilities that need to be assessed and the structured protocols that need to be followed. To lighten the elicitation burden, several methods have previously been developed to construct CPTs using a limited number of input parameters, such as InterBeta, the Ranked Nodes Method (RNM), and Functional Interpolation. In this study, the burden/accuracy trade-off of InterBeta is researched by applying the method to reconstruct previously elicited CPTs and simulated CPTs, first by comparing these CPTs to ones constructed using RNM and Functional Interpolation. After that, InterBeta extensions are proposed and tested, including an extra mean function (shifted geometric mean), the elicitation of additional middle rows, and the newly proposed extension ExtraBeta. InterBeta with parent weights is found to be the best-performing method, and the ExtraBeta extension is found to be promising and is proposed for further exploration. Full article

Thus far, the focus of research has been on employing perilla seed protein isolate (PSPI) to stabilize emulsions encapsulating hydrophobic substances, but there is a dearth of studies focusing on PSPI-stabilized double emulsions for encapsulating hydrophilic materials. This experiment investigated the environmental stability (thermal, ionic strength, and freeze–thaw stability) of PSPI-stabilized double emulsions encapsulating anthocyanins. During thermal stability experiments, the emulsion color lightened as the treatment temperature increased, whereas the microstructures of the emulsions exhibited no notable differences among the groups. The anthocyanin retention and antioxidant capacity decreased with increasing thermal treatment temperature. After thermal treatment, no creaming or separation was observed, and anthocyanin retention remained above 65% in all groups. Ionic strength exerted a certain influence on the stability of the emulsions, with droplet size increasing and anthocyanin retention dwindling as ionic strength intensified. At an ionic strength of 100 mmol/L, anthocyanin retention surpassed 70%. No delamination was observed at any of the ionic strengths. With the augmentation of freeze–thaw cycles, the emulsions darkened and yet remained unseparated, droplet size progressively increased, and anthocyanin retention progressively decreased. The findings indicate that the emulsions were environmentally stable and could serve as a reference for the development of related emulsions. Full article

Kojic acid (KA) is an economically important molecule, due to its functions as an anti-inflammatory, antifungal, and facial skin-lightening agent. Considering the wide application of this metabolite, it is essential to study processes that increase or improve its production. The objective of this study was to evaluate the effect of agitation on fungal KA production. To evaluate the effect of agitation on fungal KA production, liquid medium fermentation was carried out using batch bioreactors with a capacity of one liter. The Aspergillus oryzae strain was used, with glucose as the sole carbon source. Three experimental factors were evaluated: illumination (light or darkness), agitation type (no agitation, bubbling, and tangential), and time (0, 24, 48, 72, 96, 120, 144, 168 h). The evaluated variables included pH, product-to-biomass yield, protein content, reducing sugar consumption, and KA concentration. The bubbling level with light for 144 h showed the highest efficiency by producing 7.86 ± 2.21 g KA/L. The production of KA in liquid medium with the fungus A. oryzae requires bubbling conditions with light to achieve the best yields and production. The findings in this study provide insights into the influence of agitation conditions on KA biosynthesis and its potential for scaling up industrial fermentation. However, future work could investigate the metabolic and genetic mechanisms of this enhanced production to generate more efficient biotechnological applications for KA production. Full article

Hyperpigmentation can be prevented by regulating melanin synthesis through tyrosinase inhibition. As such, tyrosinase inhibitors like arbutin, kojic acid, and hydroquinone are commonly used for skin lightening. Recent studies suggest that certain pyrazole derivatives with tyrosinase activity may also have anticancer potential by influencing melanocyte transformation and tumor progression, positioning them as promising candidates for both cosmetic and therapeutic uses. The aim of this study was to evaluate the tyrosinase inhibitory activity of carbothioamidopyrazole derivatives. Inhibition was determined using the Dixon method, leveraging in silico molecular docking and circular dichroism (CD) spectroscopy to analyze fluorescence quenching. Carbothioamidopyrazole derivatives at the C-3 and C-5 positions in the pyrazole ring may be effective alternatives to traditional skin-lightening agents. These derivatives can induce structural changes in tyrosinase, thus altering its activity, and influence melanocyte transformation. Their dual action as tyrosinase inhibitors and potential anticancer agents makes them valuable for future research. Two compounds exhibited stronger inhibitory activity than kojic acid. Molecular docking suggests that these derivatives may block tyrosinase activity by preventing substrate access to its active site. These results underscore the potential of pyrazole derivatives for both cosmetic and therapeutic applications. Full article

In this study, two types of Cu-MOFs (Cu-TCPP and CuO-TCPP) with a two-dimensional layered porous structure were prepared via in situ polymerization using Cu²⁺, CuO, and TCPP as raw materials. Both Cu-MOFs exhibited peroxidase-like activity, capable of catalyzing the oxidation of TMB by H₂O₂ to form oxTMB, resulting in an absorption peak at 652 nm and a color change from colorless to blue. Subsequently, the addition of AA can reduce oxTMB back to TMB, causing the color of the system to lighten or become colorless. Based on this principle, a simple and rapid colorimetric method for AA detection was established and successfully applied to the detection of TAC in fruits and vegetables. The results showed that Cu-TCPP and CuO-TCPP had a large linear range of ascorbic acid detection of 0.01–100 mM (Cu-TCPP) and 0.05–100 mM (CuO-TCPP). This study not only provides a novel method for preparing nanozymes with peroxidase-like activity, but also offers a simple approach for analyzing the TAC of food. Full article

Magnoliaceae plants have high ornamental value, resulting in their widespread use in landscaping construction, and play a major role in the ecological functions of soil and water conservation. However, the landscape value of magnolias from the perspective of the water conservation capacity of the litter and soil layers is not yet fully understood; this restricts the popularization and application of Magnoliaceae plants in landscaping. In this study, we determined the characteristics of the litter thickness and mass, water absorption process, and soil water-holding capacity associated with five Magnoliaceae plants (Michelia shiluensis, M. crassipes, M. foveolata, M. maudiae, and M. odora). (1) The total litter thickness ranged from 2.29 to 5.58 cm, with M. crassipes achieving the highest value. The total litter mass for M. shiluensis (25.11 ± 2.58 t·ha⁻¹) was largely greater than that for the other magnolias. The mass of the un-decomposed litter (UL) layer was 1.31- to 3.82-fold larger than that of the semi-decomposed litter (SL). (2) The maximum water retention capacity (H_max) and effective water retention capacity (H_eff) of M. shiluensis were markedly larger than those of the other magnolias. (3) The W_max and W_eff of the UL layer were greater than those of the SL layer. (4) The soil bulk density varied from approximately 1.22 ± 0.08 g·cm⁻³ to 1.55 ± 0.08 g·cm⁻³, and the total soil porosity varied from 40.03 ± 3.44% to 46.42 ± 1.02%. The soil bulk density rose with an increasing soil depth, yet the total porosity was reduced. The soil water-holding capacity of the 0–30 cm soil layer varied from approximately 26.23 to 70.33 t·ha⁻¹, with soil near M. crassipes having the greatest water-holding capacity. The soil water infiltration recorded for M. crassipes was significantly higher than that of the other magnolias. The water conservation capacities associated with M. crassipes and M. shiluensis were the largest, which may suggest that these species are better at increasing rainfall interception, lightening splash erosion, and reducing surface runoff. Hence, we suggest that M. crassipes and M. shiluensis should be prioritized in landscaping applications. Full article

Background/Objectives: This study aimed to develop and evaluate an anti-pollution film-forming spray (FFS) containing coffee cherry pulp extract (FFS-CCS). The formulation was designed to create a protective skin barrier, improving skin health while defending against environmental pollutants. Its physical properties, dust resistance, stability, skin penetration, and clinical effectiveness were assessed to ensure optimal performance and safety. Methods: Various polymers and a ternary solvent system were used to enhance the stability and solubility of bioactive compounds from the coffee cherry pulp extract. The formulations were characterized based on appearance, film formation, viscosity, pH, spray uniformity, spray pattern, angle, film thickness, and particle adhesion. Stability testing was conducted under different storage conditions. Skin penetration was assessed using Franz diffusion cells with Strat-M^® membranes to simulate human skin. A single-blind, placebo-controlled trial with 42 participants was conducted over 60 days to evaluate the effects of FFS-CCS on skin hydration, tone, and wrinkle reduction. Clinical assessments were performed using a Corneometer, Mexameter, and Skin Visioscan. Results: The FFS1-CCS formulation, incorporating PVP K90 and a ternary solvent system, significantly improved the solubility, stability, and bioavailability of key bioactive compounds (chlorogenic acid, caffeine, and theophylline). Physical characterization confirmed uniform, transparent films with optimal viscosity and sprayability. Stability testing showed minimal degradation. Skin penetration and retention studies revealed enhanced retention of bioactive compounds with minimal systemic absorption. PVP K90, along with ethanol and propylene glycol, extended the compounds’ residence time on the skin, ensuring localized delivery. Clinically, FFS1-CCS significantly improved skin hydration, reduced roughness, lightened skin tone, and decreased erythema. Conclusions: The FFS1-CCS formulation utilizing PVP K90 significantly enhanced the stability, bioavailability, and skin retention of coffee cherry pulp extract, resulting in improved skin hydration, wrinkle reduction, and skin tone enhancement. These findings highlight the potential of coffee cherry pulp extract as a multifunctional, sustainable cosmeceutical ingredient, offering both anti-aging and environmental protection benefits, making it a promising solution for skincare applications. Full article