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35 pages, 30831 KB  
Article
Construction of Multi-Functional Composite Resilient Ecological Networks in High-Density Cities
by Hui Li, Jiaheng Du, Wanqi Guo, Qing Xu, Jinli Zhu, Zhenzhou Xu and Wei Gao
Land 2026, 15(6), 1097; https://doi.org/10.3390/land15061097 - 21 Jun 2026
Viewed by 247
Abstract
The rapid development of high-density cities has triggered severe ecological challenges, including habitat fragmentation, urban heat island (UHI) effects, and conflicting demands for public recreation. Traditional ecological networks (ENs) often focus only on “source” landscapes while neglecting degraded “sink” areas. This bias limits [...] Read more.
The rapid development of high-density cities has triggered severe ecological challenges, including habitat fragmentation, urban heat island (UHI) effects, and conflicting demands for public recreation. Traditional ecological networks (ENs) often focus only on “source” landscapes while neglecting degraded “sink” areas. This bias limits the ability of planners to resolve complex spatial conflicts. Therefore, the primary aim of this study is to develop a robust spatial planning framework that mitigates urban ecological conflicts and enhances regional resilience. To achieve this, we constructed a composite ecological network (CEN) for the high-density city of Guangzhou that harmonizes bird habitat conservation, thermal regulation, and cultural recreation. We combined the MaxEnt model, morphological spatial pattern analysis (MSPA), and circuit theory to identify functional “sources” and “sinks” across these three dimensions. Next, using complex network theory, we optimized the CEN and evaluated its structural robustness using low degree addition (LDA) and low betweenness addition (LBA) strategies. The results indicate the following: (1) The CEN effectively captured the complex mosaic landscape of the city. (2) Single-objective networks displayed distinct spatial differences—the recreational network formed a dispersed web of 242 corridors, while habitat and climate networks remained highly clustered. (3) The integrated CEN generated 1137 multi-layered corridors, creating a vital green skeleton to support species dispersal, mitigate UHI effects, and improve cultural access. (4) Optimization simulations verified that the LBA strategy provided the highest stability against targeted attacks by balancing network connectivity with local aggregation. Ultimately, this framework offers a highly adaptable planning tool for dense cities, providing precise spatial guidance to overcome ecological bottlenecks and harmonize urban growth with ecosystem resilience. Full article
(This article belongs to the Special Issue Ecology of the Landscape Capital and Urban Capital—Second Edition)
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28 pages, 22867 KB  
Article
Quantifying Categorical Information Loss in Forest Compositional Mapping: Implications for the Accuracy of Forest Assessment in Lualaba Province (DR Congo)
by Médard Mpanda Mukenza, John Kikuni Tchowa, Felana Nantenaina Ramalason, Heritier Khoji Muteya, Jan Bogaert, Yannick Useni Sikuzani and Jean-François Bastin
Remote Sens. 2026, 18(12), 1979; https://doi.org/10.3390/rs18121979 - 14 Jun 2026
Viewed by 225
Abstract
Forests of Lualaba Province (DR Congo) form a compositionally complex mosaic of dry dense forest, gallery forest, and Miombo woodland. Yet, categorical land-cover maps impose discrete boundaries on these inherently continuous vegetation gradients, systematically discarding subpixel compositional information critical for forest monitoring and [...] Read more.
Forests of Lualaba Province (DR Congo) form a compositionally complex mosaic of dry dense forest, gallery forest, and Miombo woodland. Yet, categorical land-cover maps impose discrete boundaries on these inherently continuous vegetation gradients, systematically discarding subpixel compositional information critical for forest monitoring and carbon accounting. The magnitude of this information loss at the landscape scale, however, remains largely unquantified. In this study, we train a Multi-Output Neural Network (MONN) using Sentinel-2 spectral and textural predictors (2025) to estimate the proportional cover of three forest types across the province. Model performance is benchmarked against a normalised Random Forest (RF) using spatial block cross-validation. Categorical information loss is quantified pixel-wise using two complementary metrics, dominant class proportion and Shannon compositional entropy, alongside a derived interpretive quantity, categorical information loss. The MONN slightly outperformed RF (R2 = 0.648 vs. 0.630; RMSE = 0.224 vs. 0.229), yet the results reveal a fundamentally heterogeneous landscape structure. The mean dominant-class proportion was only 56.2%, indicating that categorical maps discard, on average, 43.8% of compositional information per pixel. Only 7.9% of forested pixels exceeded the 75% dominance threshold, while Shannon entropy reached 74.1% of its theoretical maximum, indicating that forest types coexist in near-equal proportions across most pixels. This renders categorical attribution structurally inadequate for most of the forested landscape. Across 92.1% of forested pixels, no single forest type achieved clear dominance. These results show that compositional mixing is the dominant structural condition of the landscape, and that compositional mapping is essential for representing tropical forest structure in heterogeneous drylands. By formally quantifying categorical information loss at the landscape scale, this study shows that continuous compositional mapping converts this structural ambiguity into a spatially explicit ecological signal, with direct implications for monitoring vegetation dynamics and biodiversity, suggesting a structural source of error in carbon stock estimation in tropical dry forests that warrants empirical validation. Full article
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19 pages, 3629 KB  
Article
Habitat-Associated Dietary Plasticity in the Japanese Weasel (Mustela itatsi): Fecal Analysis in a Floodplain Wetland and Comparative Synthesis
by Shufan Qiao, Kaoru Suzuki, Masato Yoshikawa, Chris Newman and Yayoi Kaneko
Animals 2026, 16(11), 1720; https://doi.org/10.3390/ani16111720 - 4 Jun 2026
Viewed by 703
Abstract
The range of food resources consumed by opportunistic carnivores is shaped by habitat-specific availability and productivity. However, baseline data on the diet of the Japanese weasel (Mustela itatsi) in managed wetland habitats remain scarce. Firstly, we conducted a diet study examining [...] Read more.
The range of food resources consumed by opportunistic carnivores is shaped by habitat-specific availability and productivity. However, baseline data on the diet of the Japanese weasel (Mustela itatsi) in managed wetland habitats remain scarce. Firstly, we conducted a diet study examining Japanese weasel scats from the Watarase-yusuichi wetland (WYW) (September 2024–August 2025). Based on 103 fecal samples, we calculated seasonal frequency of occurrence (FO) for 16 food categories and estimated their relative volume (RV) using the point-frame method. Surprisingly, plant material and fruit seeds were eaten frequently across seasons, and fruit seeds constituted a major component of scat volume, particularly in summer. Secondly, we compared baseline WYW data with published datasets from two contrasting aquatic habitats (a suburban river area and a rice paddy landscape) using harmonized categories and multivariate analyses. Diet composition differed markedly among study sites, and the PERMANOVA analysis indicated a significant effect of sampling site (R2 = 0.643, p = 0.001) with a weaker seasonal effect (R2 = 0.208, p = 0.038), with no significant pairwise differences between seasons (all p ≥ 0.300). Although cross-site comparisons should be interpreted cautiously because datasets were collected in different decades (1998, 2018, and 2024–2025), these findings support habitat-associated trophic plasticity in the Japanese weasel, with dietary shifts across habitats and seasons apparently reflecting variation in available food resources. In managed floodplain wetlands such as WYW, maintaining a mosaic of reed beds, ponds, and channels may help sustain dietary flexibility and population persistence in this species. Full article
(This article belongs to the Section Ecology and Conservation)
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20 pages, 3829 KB  
Article
Vegetation Mosaic Effects on Soil Microbial Community Structure and Enzyme Functioning in Relation to Nutrient Heterogeneity in a Mountainous Ecotone
by Gang Lei, Yang Yang, Wenting Li, Tian Chen and Lianghua Qi
Plants 2026, 15(11), 1672; https://doi.org/10.3390/plants15111672 - 29 May 2026
Viewed by 671
Abstract
Vegetation mosaics characterize mountainous agroforestry ecosystems, yet how their spatial configuration shapes soil microbial assembly and functions remains unresolved. This study investigated how mosaic elements (monocultures, shrublands, and ecotones) drive microbial communities and enzyme activities across a forest–shrubland–farmland mosaic in western Hunan, China. [...] Read more.
Vegetation mosaics characterize mountainous agroforestry ecosystems, yet how their spatial configuration shapes soil microbial assembly and functions remains unresolved. This study investigated how mosaic elements (monocultures, shrublands, and ecotones) drive microbial communities and enzyme activities across a forest–shrubland–farmland mosaic in western Hunan, China. Nutrient stoichiometry, microbial biomass (PLFA), and six enzyme activities were analyzed via variance partitioning, partial least squares regression, and ordination analysis. Fungal biomass dominated, peaking in ecotones and showing the lowest values in monocultures and shrublands. Microbial assembly was regulated by soil nutrients (31%) rather than soil texture (15%). Fungi (variable importance in projection, VIP = 1.287) and bacteria (VIP = 1.003) were key drivers, indicating distinct functional compartmentalization: fungi drove oxidative enzymes, whereas bacteria mediated nutrient cycling. Actinomycetes and total PLFA acted as secondary drivers, with VIP values of 0.932 and 0.939, respectively. Soil organic matter, dissolved organic carbon, silt content, and available nitrogen were key abiotic predictors. Collectively, vegetation configuration regulates soil functioning via nutrient-mediated microbial assembly and functional differentiation across mosaic elements. These findings underscore the role of landscape heterogeneity in sustaining soil fertility, suggesting that protecting ecotones and maintaining mosaic complexity should be prioritized in mountainous agroforestry management to enhance soil ecological functioning under global land-use change. Full article
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36 pages, 10342 KB  
Article
Rural Landscapes Under Real Estate Pressure: The Overflowing City
by Maria Rosa Trovato, Chiara Minioto, Salvatore Giuffrida and Ludovica Nasca
Real Estate 2026, 3(2), 5; https://doi.org/10.3390/realestate3020005 - 18 May 2026
Viewed by 296
Abstract
This research examines how the relationship between cities and rural areas has evolved in light of the profound transformation affecting rural areas of high landscape value, which has been driven by the expansion opportunities granted to the real estate sector by urban planning [...] Read more.
This research examines how the relationship between cities and rural areas has evolved in light of the profound transformation affecting rural areas of high landscape value, which has been driven by the expansion opportunities granted to the real estate sector by urban planning regulations. The role of the landscape dimension in interpreting the relationship between territorial wealth and landscape value is considered, based on the convergence of two complementary disciplinary perspectives on territory: land planning and valuation science. Against this backdrop, and with a view to containing the progressive contamination of rural and agricultural heritage by the real estate sector, this study proposes a structured observation, valuation, interpretation, and regulatory tool to support the development of territorial planning in areas significantly characterized in terms of rural landscape value. The proposed tool is based on evidence regarding the phenomenon of building expansion in the agricultural territory of a municipality in southeastern Sicily, where favorable conditions for the development of the building sector exist, such as the vastness of the municipal territory and extensive farming as the mainstay of agricultural activity. This wider sub-regional area has also received attention due to the over-tourism phenomenon that has occurred in its cities of art. The evaluation approach experienced is a value-based representation of the evolution of this process over three observation periods: 2000, 2007, and 2012, relating the quantitative observation of the building expansion to the connected qualitative impact on rural landscape. It is the result of coordinating a large set of data in a hierarchical model of indices that converge to construct a synthetic index of rural landscape resilience. This achievement is based on the linguistic progression of “lexicon”, “semantics”, “syntax”, and “pragmatics”, each of which robustly supports “observation”, “valuation”, “interpretation”, and “planning”, respectively. The final stage is based on the convergence of explanatory indices, which are developed by coordinating evidence and assessments (factual and value judgements). This stage enables the proposal of a constraints system that supports a modus vivendi between the interests of the real estate sector and the values of the rural landscape in such a rich and fragile area. Full article
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13 pages, 4175 KB  
Article
Habitat Structure Outweighs Monastic Legacy in Shaping Bird Assemblages
by Łukasz Jankowiak, Kinga Piórkowska, Michał Polakowski, Sebastian Michałowski and Michał Szkudlarek
Animals 2026, 16(10), 1534; https://doi.org/10.3390/ani16101534 - 17 May 2026
Viewed by 764
Abstract
Sacred natural sites are often considered potential refugia for biodiversity in human-dominated landscapes, but their effects can be confounded by present-day habitat structure. We tested whether extant Cistercian monasteries in western Poland influence breeding-bird assemblages at two spatial scales by conducting standardized 5 [...] Read more.
Sacred natural sites are often considered potential refugia for biodiversity in human-dominated landscapes, but their effects can be confounded by present-day habitat structure. We tested whether extant Cistercian monasteries in western Poland influence breeding-bird assemblages at two spatial scales by conducting standardized 5 min point counts during two visits at 234 stations across 23 plots and comparing Cistercian plots with environmentally matched Control and Post-Cistercian plots. We recorded 133 breeding-bird species, numerically dominated by widespread farmland and synanthropic taxa. Neither plot category nor station placement within versus outside monastery grounds explained variation in Shannon diversity or rarefied species richness. In contrast, both diversity metrics increased with contemporary landscape complexity, especially along gradients from arable land toward grasslands and urban habitats and with increasing heterogeneous agriculture, while community composition was significantly associated with current landcover structure. These findings indicate that present-day habitat structure, rather than monastic legacy, is the main driver of breeding-bird diversity in this system. Conservation and land-use policy in agricultural regions should therefore prioritize the maintenance and restoration of heterogeneous landscapes, including mosaics of semi-natural habitat elements. Full article
(This article belongs to the Section Birds)
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19 pages, 50286 KB  
Article
The Joint Effects of Habitat Types and Surrounding Landscape Patterns on the Diversity of True Bugs in Southwest China
by Shutong Gao, Zhixing Lu, Xiang Zhang, Qiao Li and Youqing Chen
Insects 2026, 17(5), 497; https://doi.org/10.3390/insects17050497 - 13 May 2026
Viewed by 365
Abstract
Understanding the drivers of arthropod community diversity is essential for effective biodiversity conservation, particularly in human-dominated landscapes. True bugs (Hemiptera) are morphologically and ecologically diverse, differing in feeding strategy, specialization, and mobility, and thus occupy a wide range of habitats. We sampled true [...] Read more.
Understanding the drivers of arthropod community diversity is essential for effective biodiversity conservation, particularly in human-dominated landscapes. True bugs (Hemiptera) are morphologically and ecologically diverse, differing in feeding strategy, specialization, and mobility, and thus occupy a wide range of habitats. We sampled true bugs using sweep nets across 257 plots in the Xishuangbanna Biodiversity Priority Area, Southwest China, including natural forests, planted forests, cultivated lands, and complex habitats comprising mosaics of these land-use types. We quantified morphological traits and assessed how habitat type and surrounding landscape structure at multiple spatial scales influence taxonomic and functional diversity. Species richness and abundance were lowest in cultivated lands, whereas functional diversity remained relatively consistent across habitats. Landscape composition and configuration significantly shaped community structure, with effects varying by habitat type and spatial scale. Overall, landscape heterogeneity promoted species richness and abundance, while connectivity and fragmentation showed contrasting effects depending on habitat context. Complex habitats and forests generally supported higher diversity under heterogeneous and well-connected landscapes. These results demonstrate that habitat characteristics and landscape structure jointly regulate true bug diversity, highlighting the need for habitat-specific landscape management that enhances heterogeneity and connectivity in natural and complex habitats while reducing isolation and simplification in human-modified systems. Full article
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20 pages, 13993 KB  
Review
Farmland Abandonment and High Nature Value Farming in Mediterranean Landscapes: Plant Biodiversity Outcomes and Biocultural Trade-Offs
by Alexandra D. Solomou
Land 2026, 15(5), 793; https://doi.org/10.3390/land15050793 - 8 May 2026
Viewed by 291
Abstract
Mediterranean rural landscapes are globally important biocultural systems in which long-standing low-intensity farming, grazing, terracing, and agroforestry have historically maintained fine-grained habitat mosaics and high vascular-plant diversity. This review adopts a systematic scoping approach focused on farmland abandonment and High Nature Value (HNV) [...] Read more.
Mediterranean rural landscapes are globally important biocultural systems in which long-standing low-intensity farming, grazing, terracing, and agroforestry have historically maintained fine-grained habitat mosaics and high vascular-plant diversity. This review adopts a systematic scoping approach focused on farmland abandonment and High Nature Value (HNV) farming, with a search window extending from 1996 to April 2026. Following PRISMA 2020 and PRISMA-ScR principles, the review maps evidence on plant richness, diversity indices, floristic turnover, indicator species, and vegetation trajectories across Mediterranean agricultural and semi-natural systems. The literature shows that abandonment does not produce a single biodiversity outcome. Early abandonment can maintain or temporarily increase local richness through overlap among remnant grassland species, annuals, and colonizing shrubs, whereas prolonged abandonment more often drives shrub encroachment, woody dominance, and losses of open-habitat specialists. By contrast, HNV systems such as extensive grazing, agro-pastoral mosaics, wood pastures, and dehesa/montado-like agroforestry more consistently maintain habitat interfaces, beta diversity, and plant assemblages associated with cultural landscape continuity. The review argues that biodiversity-sensitive policy in the Mediterranean should move beyond the binary of abandonment versus conservation, and instead support landscape-specific combinations of passive succession, targeted grazing, agroforestry renewal, and rural livelihood viability. Full article
(This article belongs to the Special Issue Rural Space: Between Renewal Processes and Preservation)
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24 pages, 3352 KB  
Article
Integrating Land Use and Poaching Impacts for Sustainable Wildlife Management in the Atlantic Forest of Misiones, Argentina
by Delfina Sotorres, Carina F. Argüelles, Orlando M. Escalante, Miguel A. Rinas and Karen E. DeMatteo
Sustainability 2026, 18(9), 4329; https://doi.org/10.3390/su18094329 - 27 Apr 2026
Viewed by 832
Abstract
Misiones, Argentina, holds one of the largest remnants of the Atlantic Forest, with almost 1.4 million hectares of native forest, representing a critical landscape for sustainable biodiversity conservation. However, connectivity across this ecoregion is increasingly threatened by habitat conversion, landscape fragmentation, and poaching [...] Read more.
Misiones, Argentina, holds one of the largest remnants of the Atlantic Forest, with almost 1.4 million hectares of native forest, representing a critical landscape for sustainable biodiversity conservation. However, connectivity across this ecoregion is increasingly threatened by habitat conversion, landscape fragmentation, and poaching pressures that extend beyond protected area boundaries, undermining long-term sustainability of wildlife populations. Using conservation detection dogs, we located, collected, and genetically confirmed 198 scats belonging to four game species: 20 lowland tapir (Tapirus terrestris), 72 white-lipped peccary (Tayassu pecari), 55 collared peccary (Pecari tajacu), and 51 Azara’s agouti (Dasyprocta azarae). Analyses examining species-specific habitat associations emphasized the importance of extending inference beyond point locations to encompass species’ home ranges, with native forest consistently identified as a key component of habitat use. The high prevalence of scats in mosaics of human-modified habitats outside protected areas, especially along their borders, underscores the importance of managing these areas as part of a broader sustainable landscape matrix. While native forest fragments outside of protected areas may serve as important refugia supporting species persistence, their contribution to sustainable management depends on reducing poaching pressure across these landscapes. There is an urgent need to expand antipoaching efforts beyond protected areas and across the Atlantic Forest in the Green Corridor of Misiones while preventing ongoing deforestation and the expansion of monoculture plantations. Achieving sustainable wildlife management in this region will require integrated strategies that promote sustainable land use, conservation planning, and rural development. Full article
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26 pages, 14980 KB  
Article
Dynamic Conflict Footprints and Land-System Transformation in Large-Scale Mining: Evidence from Las Bambas, Peru
by Soledad Espezúa, Rodrigo Caballero, Álvaro Talavera and Luciano Stucchi
Land 2026, 15(5), 698; https://doi.org/10.3390/land15050698 - 22 Apr 2026
Cited by 1 | Viewed by 532
Abstract
Socio-environmental conflicts in mining regions are often examined through political, economic, or social lenses, while the role of land-system transformation remains less integrated into quantitative analysis. This study examines the co-evolution of socio-environmental conflict and territorial change in Las Bambas (Apurímac, Peru) as [...] Read more.
Socio-environmental conflicts in mining regions are often examined through political, economic, or social lenses, while the role of land-system transformation remains less integrated into quantitative analysis. This study examines the co-evolution of socio-environmental conflict and territorial change in Las Bambas (Apurímac, Peru) as a socio-territorial process. Annual conflict records from the Peruvian Ombudsman’s Office (2007–2024) were combined with annual land-cover data from MapBiomas. Yearly conflict influence zones were reconstructed from reported affected communities and geographic features using buffered spatial entities and concave hull polygons. Clustering methods (K-medoids, DBSCAN, and agglomerative hierarchical clustering) and FP-Growth association rule mining were applied to 23 unique conflicts consolidated from the original records and encoded with 10 root causes. The most intense conflict phases were accompanied by measurable landscape transformations, including the emergence of mining-related land cover from 2012 onward, sustained loss of high-Andean natural vegetation, expansion of agricultural mosaics, urban growth along the Apurímac–Cusco corridor, and hydrological alterations in wetlands and headwaters. Three conflict typologies were identified, with unfulfilled company commitments emerging as the most recurrent co-occurring grievance. The dynamic polygon approach offers a replicable framework for linking conflict records with land-system change in extractive regions. Full article
(This article belongs to the Section Land Systems and Global Change)
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17 pages, 567 KB  
Review
A Scoping Review of the Relationship Between Play and Learning Beyond Preschool
by Jaydene Barnes, Tonia Gray and Christine Woodrow
Educ. Sci. 2026, 16(4), 633; https://doi.org/10.3390/educsci16040633 - 16 Apr 2026
Viewed by 1159
Abstract
Internationally, there are increased pressures for primary schools to meet academic curriculum outcomes primarily driven by performance metrics and targets. Sitting alongside this context are competing concerns for the decline in children’s play opportunities to bolster their overall health and wellbeing. Adopting play-based [...] Read more.
Internationally, there are increased pressures for primary schools to meet academic curriculum outcomes primarily driven by performance metrics and targets. Sitting alongside this context are competing concerns for the decline in children’s play opportunities to bolster their overall health and wellbeing. Adopting play-based pedagogies in primary schools can infuse more play into children’s lives whilst meeting curriculum outcomes. Despite the perceived importance of play during childhood, play-based pedagogies are still mostly positioned as legitimate pedagogical approaches in prior to school settings. Given this landscape, this research seeks to understand contemporary educational research of play-based pedagogies in primary schools by conducting a scoping review. Through presenting a narrative account of the literature, and synthesising these ideas into broader themes, the research identified that there remains international interest in play-based pedagogies in the primary years of school but despite this, questions surrounding its legitimacy remain. This review and subsequent discussion surface potential next steps including a recommendation to increase empirical research on the adoption of play-based pedagogies in schools with consideration of using a ’Mosaic approach’ to data collection, as well as research focusing on the active and intentional role of the teacher. Lastly, as a way forward, the research brings to light the potential of creating a ‘space’ for the merging of two knowledge systems from two often siloed approaches to education—early childhood and primary—to create a new pathway. Such a pathway has potential to support continuity of learning, student engagement, children’s health, and wellbeing. Full article
(This article belongs to the Special Issue Learning Through Play: Reimagining Pedagogies in Early Childhood)
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21 pages, 11364 KB  
Article
Severity-Driven Assessment of Greenhouse Gas Emissions from Large Mediterranean Wildfires Using Remote Sensing and Vegetation Mosaics
by Helena van den Berg Sesma, Edgar Lorenzo-Sáez, Victoria Lerma-Arce, Jose-Vicente Oliver-Villanueva and Mauricio Acuna
Fire 2026, 9(4), 167; https://doi.org/10.3390/fire9040167 - 14 Apr 2026
Viewed by 1629
Abstract
Estimating wildfire greenhouse gas (GHG) emissions in Mediterranean landscapes is challenging due to heterogeneous fuel mosaics and limited scalability of field-based approaches. This study presents a Geographic Information System (GIS) based framework that integrates land-cover data, pre-fire biomass estimates, fire severity mapping, and [...] Read more.
Estimating wildfire greenhouse gas (GHG) emissions in Mediterranean landscapes is challenging due to heterogeneous fuel mosaics and limited scalability of field-based approaches. This study presents a Geographic Information System (GIS) based framework that integrates land-cover data, pre-fire biomass estimates, fire severity mapping, and established emission factors to produce spatially explicit estimates of biomass consumption and GHG emissions. Fire severity was derived from multitemporal Sentinel-2 imagery using the differenced Normalized Burn Ratio (ΔNBR) and combined with land-cover information to define vegetation–severity classes for emission estimation. A key innovation is the identification of co-occurring vegetation types within the same spatial units, allowing emissions to be quantified across vegetation mixtures rather than single classes, providing a more realistic representation of Mediterranean forests. Applied to the 2022 Bejis wildfire, pre-fire biomass within the burned area was 673,601 tons. Coniferous forests dominated, but co-occurrence with shrubland and herbaceous layers produced the highest emission contributions, highlighting the role of vegetation interactions. Total emissions were estimated at 625,938 tons of equivalent CO2, and comparison with large-scale datasets (CAMS Global Fire Assimilation System, Global Fire Emissions Database) shows general coherence. This severity-driven, vegetation-explicit framework demonstrates robust potential for quantifying wildfire emissions across heterogeneous Mediterranean landscapes, though uncertainties remain due to pre-defined biomass, burning efficiency, emission factors, assumptions in fire severity mapping, and limited field validation. The approach can support improved regional GHG inventories and wildfire management strategies. Full article
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24 pages, 2964 KB  
Review
Semi-Natural Dry Grasslands in Decline: A Review of Characteristics, Threats and Conservation Challenges
by Justyna Wielgos and Mariusz Kulik
Diversity 2026, 18(4), 216; https://doi.org/10.3390/d18040216 - 8 Apr 2026
Viewed by 1069
Abstract
In Europe, the most valuable grasslands are semi-natural ecosystems maintained by long-term extensive human management, particularly pastoralism, and therefore do not represent climax vegetation. According to the Natura 2000 habitat interpretation manual (EUR-28), key habitats include xerothermic grasslands of Festuco-Brometalia (code 6210*) on [...] Read more.
In Europe, the most valuable grasslands are semi-natural ecosystems maintained by long-term extensive human management, particularly pastoralism, and therefore do not represent climax vegetation. According to the Natura 2000 habitat interpretation manual (EUR-28), key habitats include xerothermic grasslands of Festuco-Brometalia (code 6210*) on calcareous soils and sandy grasslands of Koelerion glaucae (code 6120*) on poor substrates. Only 10–15% of their area in the EU has favorable conservation status. The main threat is secondary succession and encroachment (83.94%), caused by abandonment of traditional management (81.75%). Without mowing or grazing, dominant grasses replace rare species, followed by shrubs and trees. Other pressures include intensive agriculture (75.18%), habitat loss and fragmentation (69.34%), climate change (37.96%), invasive species (23.36%) and urbanization (14.60%). Multiple threats often co-occur, so cumulative percentages exceed 100%. The most effective conservation method is restoring or maintaining extensive grazing, particularly with local sheep and goat breeds. Grazing limits succession, increases structural diversity and promotes seed dispersal, creating a mosaic of microhabitats that enhances biodiversity. Effective protection requires landscape-scale actions, limiting urban development, and long-term support for farmers under the Common Agricultural Policy. Increasing public awareness of the ecological and cultural value of these ecosystems is also essential. Full article
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20 pages, 1189 KB  
Review
The Feasibility of Developing a Universal SARS-CoV-2 Vaccine
by Mohammed Asaad, Mohamed O. Mustafa, Yaman Al-Haneedi, Lina Shalaby, Rania shams Eldin, Yasar Mohamedahmed, Hadi M. Yassine, Abdallah M. Abdallah and Mohamed M. Emara
Vaccines 2026, 14(3), 259; https://doi.org/10.3390/vaccines14030259 - 13 Mar 2026
Viewed by 2117
Abstract
As SARS-CoV-2 continues to evolve with increased transmissibility and immune evasion, the need for vaccines that provide broader and more durable protection has become increasingly urgent. The extensive research spurred by the pandemic has accelerated the development of diverse vaccine platforms, including mRNA, [...] Read more.
As SARS-CoV-2 continues to evolve with increased transmissibility and immune evasion, the need for vaccines that provide broader and more durable protection has become increasingly urgent. The extensive research spurred by the pandemic has accelerated the development of diverse vaccine platforms, including mRNA, DNA, virus-like particles (VLPs), recombinant proteins, and mosaic mono- and polyvalent vaccines. While several of these platforms have reached regulatory approval and widespread clinical employment, others remain under evaluation or in various stages of clinical development. These vaccines have significantly reduced infection rates, severe disease, and hospitalizations, particularly among high-risk group. Nevertheless, the ongoing emergence of novel variants and subvariants has challenged the efficacy of both existing and newly developed vaccines. This evolving landscape underscores the urgent need for a universal SARS-CoV-2 vaccine platform capable of providing comprehensive and long-lasting immunity. In this review, we evaluate current and emerging strategies for SARS-CoV-2 universal vaccine development, with a focus on antigen design, breadth of immune protection, and clinical feasibility. Attention is given to various universal vaccine platforms such as the mosaic polyvalent spike construct, multi-epitope vaccines targeting the receptor-binding domain (RBD), and approaches centered on the conserved S2 subunit of the spike protein. We also discuss strategies leveraging additional conserved viral proteins and T helper (Th) and cytotoxic T lymphocyte (CTL) epitopes from across coronaviruses. By highlighting the advances in these areas, this review provides a framework to guide the rational design of next-generation universal vaccines capable of delivering broad and durable protection against SARS-CoV-2 variants. Full article
(This article belongs to the Collection COVID-19 Vaccine Development and Vaccination)
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17 pages, 33308 KB  
Article
Mapping of Threatened Vereda Wetlands in the Brazilian Midwest Using a Domain-Specific U-Net
by Jeaneth Machicao, Alexandre Augusto Barbosa, Leandro O. Salles, Peter Mann Toledo, Pedro Luiz P. Corrêa, Luiz Flamarion B. Oliveira, Rosane Garcia Collevatti, Eduardo Barroso de Souza and Jean Pierre H. B. Ometto
Remote Sens. 2026, 18(5), 791; https://doi.org/10.3390/rs18050791 - 5 Mar 2026
Viewed by 617
Abstract
The palm swamp landscapes, particularly the Vereda wetlands and their associated swamp gallery forests (VED.SGF), comprise essential yet threatened ecosystems within the Brazilian Cerrado. In addition to supporting significant portions of biodiversity, they provide critical ecosystem services such as storing and filtering excess [...] Read more.
The palm swamp landscapes, particularly the Vereda wetlands and their associated swamp gallery forests (VED.SGF), comprise essential yet threatened ecosystems within the Brazilian Cerrado. In addition to supporting significant portions of biodiversity, they provide critical ecosystem services such as storing and filtering excess rainwater and serving as major carbon reservoirs in organic soils. These wetlands are directly linked to the drainage systems of the headwaters of the main Cerrado river basins, which together account for about two-thirds of Brazil’s hydrographic basins. Mapping and managing VED.SGF ecosystems through remote sensing present major challenges addressed in this first study. Their narrow, dendritic, and complex tabular spatial pattern, often elongated along watersheds on scales of hundreds of kilometers, suffering distortions due to human impact, and the limited amount of annotated data make segmentation particularly challenging. Existing deep learning (DL) methods, typically pre-trained on natural images, struggle to capture the spectral and spatial intricacies of these ecosystems. This study introduces a trained-from-scratch U-Net model supported by field-based experimental procedures to ensure high-quality wetland annotations. The resulting dataset covers approximately 7300 km2 in western Bahia and provides domain-specific weights tailored to remote sensing applications. Using high-resolution (4.6 m) RGB mosaics, the model was trained, validated, and tested to establish a reproducible and scalable pipeline. The proposed method achieved robust results in an independent test area of 8040 km2, with a mean IoU of 0.728, F1-score of 0.843, and Cohen’s Kappa of 0.837. These results demonstrate consistent performance and strong generalization to new areas, establishing a scientifically reliable baseline that situates the model competitively within the current state of the art. By releasing both the model weights and annotated dataset, this study provides valuable resources to advance future research on mapping and monitoring these unique and strategic wetland ecosystems. Full article
(This article belongs to the Special Issue Intelligent Remote Sensing for Wetland Mapping and Monitoring)
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