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Communication

54 Citations

12,425 Views

19 Pages

Direct Injection of CRISPR/Cas9-Related mRNA into Cytoplasm of Parthenogenetically Activated Porcine Oocytes Causes Frequent Mosaicism for Indel Mutations

Masahiro Sato,
Miyu Koriyama,
Satoshi Watanabe,
Masato Ohtsuka,
Takayuki Sakurai,
Emi Inada,
Issei Saitoh,
Shingo Nakamura and
Kazuchika Miyoshi

Int. J. Mol. Sci.2015, 16(8), 17838-17856;https://doi.org/10.3390/ijms160817838

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3 August 2015

Some reports demonstrated successful genome editing in pigs by one-step zygote microinjection of mRNA of CRISPR/Cas9-related components. Given the relatively long gestation periods and the high cost of housing, the establishment of a single blastocys...

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Article

7 Citations

5,558 Views

19 Pages

Efficient Generation of Somatic Cell Nuclear Transfer-Competent Porcine Cells with Mutated Alleles at Multiple Target Loci by Using CRISPR/Cas9 Combined with Targeted Toxin-Based Selection System

Masahiro Sato,
Kazuchika Miyoshi,
Shingo Nakamura,
Masato Ohtsuka,
Takayuki Sakurai,
Satoshi Watanabe,
Hiroaki Kawaguchi and
Akihide Tanimoto

Int. J. Mol. Sci.2017, 18(12), 2610;https://doi.org/10.3390/ijms18122610

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4 December 2017

The recent advancement in genome editing such a CRISPR/Cas9 system has enabled isolation of cells with knocked multiple alleles through a one-step transfection. Somatic cell nuclear transfer (SCNT) has been frequently employed as one of the efficient...

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Article

10 Citations

6,018 Views

15 Pages

The Combinational Use of CRISPR/Cas9 and Targeted Toxin Technology Enables Efficient Isolation of Bi-Allelic Knockout Non-Human Mammalian Clones

Satoshi Watanabe,
Takayuki Sakurai,
Shingo Nakamura,
Kazuchika Miyoshi and
Masahiro Sato

Int. J. Mol. Sci.2018, 19(4), 1075;https://doi.org/10.3390/ijms19041075

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4 April 2018

Recent advances in genome editing systems such as clustered regularly interspaced short palindromic repeats/CRISPR-associated protein-9 nuclease (CRISPR/Cas9) have facilitated genomic modification in mammalian cells. However, most systems employ tran...

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