Search Results (49)

This study presents a strategy for enhancing hydrogel formation through SpyCatcher-mediated conjugation of nanoscale scaffold proteins. We demonstrate that SpyCatcher can facilitate hydrogel assembly with various nano-scaffolds of diverse structural configurations. By conjugating one, two, or three SpyCatcher units to the P9 protein nanoscaffold, hydrogel yield was substantially increased, allowing for the simultaneous co-immobilization of a larger number of enzymes. Characterization using cell-free biosynthesis, electron microscopy, and rheological analysis revealed that the resulting SpyCatcher-mediated nanoscaffold hydrogels exhibit soft solid-like behavior, high elasticity, and an “ink-bottle” pore morphology, which collectively promote and regulate enzymatic activity. Notably, hydrogels crosslinked via the P9 scaffold with two SpyCatcher units showed the most balanced properties, leading to a 149% increase in pyruvic acid production. These findings not only advance the efficient design of hydrogels for enzyme co-immobilization but also provide a foundation for developing more sophisticated models and expanding the scope of biocatalytic systems. Full article

The Erdaoling Mining area, located in Inner Mongolia, NW China, is recognized for its considerable potential in coalbed methane (CBM) exploration and development. However, the complex structures in this region have significant influences on coal reservoir characteristics, particularly pore structure features. This study focuses on the No. 2 coal seam of the Middle Jurassic Yan’an Formation. Three structural patterns were classified based on the existing structural characteristics of the study area. Coal samples of No. 2 coal seam were collected from different structural positions, and were subjected to low-temperature CO₂ adsorption (LTCO₂A), low-temperature N₂ adsorption/desorption (LTN₂A), low-field nuclear magnetic resonance (LF-NMR), and scanning electron microscopy (SEM) experiments, so that the structural controlling effects on pore structure would be revealed. Quantitative analysis results indicate that in terms of asymmetric syncline, from the limb to the core, the total porosity and movable fluid porosity of the coal decreased by 1.47% and 0.31%, respectively, reaching their lowest values at the core. Meanwhile, the dominant pore type shifted from primarily one-end closed pores to “ink-bottle” pores, indicating increased pore complexity. In the fold-thrust structure, the micropore specific surface area, micropore volume, mesopore specific surface area, mesopore volume, and total porosity show clear correlations with variations in coal seam structure. These parameters all reach their maximum values in the fault-cut zone at the center of the syncline, measuring 268.26 m²/g, 0.082 cm³/g, 0.601 m²/g, 1.262 cm³/g, and 4.2%, respectively. Simple pore types, like gas pores and vesicular pores, were identified in the syncline limbs, while open pores, “ink-bottle” pores, and complex multiporous types were mainly developed at fault locations, indicating that faults significantly increase the complexity of coal reservoir pore types. For the broad and gentle syncline and small-scale reverse fault combination, porosity exhibits a decreasing trend from the syncline limbs toward the core. Specifically, the mesopore specific surface area and movable fluid porosity increased by 52.24% and 43.69%, respectively, though no significant effect on micropores was observed. The syncline core in this structural setting developed normal gas pore clusters and tissue pores, with no occurrence of highly complex or heterogeneous pore types, indicating that neither the broad gentle syncline nor the small-scale faulting significantly altered the pore morphology. Comparatively, the broad and gentle syncline and small-scale reverse fault combination was determined to exert the strongest modification on pore structures of coal reservoir, followed by the asymmetric syncline, while the broad syncline alone demonstrated minimal influence. Full article

Background: The incidence of chemical ocular trauma after accidentally instilling the “wrong” eyedrops is still frequent, but cases resulting from stamper ink refills are rare. Case Presentation: A 73-year-old man presented to our emergency department with a history of inadvertently instilling stamper ink refill into both eyes (BEs) instead of artificial tears. Immediate irrigation and evaluation were performed. The initial visual acuity (VA) was 0.4 in the right eye (RE) and 0.8 in the left eye (LE). Slit lamp examination showed edema palpebra with periocular blue staining in BEs, chemotic conjunctiva with a much darker color in the RE than the LE, and epithelial defects with a positive fluorescein test in BEs. A diagnosis of bilateral corneal abrasion and chemotic conjunctiva was established. Ten hours after the emergency visit, RE VA decreased to 0.2, and corneal edema was found during the follow-up examination. Medications including levofloxacin antibiotic, sodium hyaluronate, sodium chloride, combined polymyxin sulfate–neomycin sulfate and dexamethasone eyedrops, mefenamic acid, and ascorbic acid tablets were prescribed. The RE corneal edema still occurred, and the endothelial cell count was 1952 and 987 cells/mm² in the RE and LE at the one-week follow-up. After three weeks, corneal edema had fully resolved, and the VA was 0.4 and 0.8 in the RE and LE, respectively. Conclusions: This case report adds to the spectrum of the continuing problem of chemical ocular trauma after mistakenly instilling the eyedrops. Promoting and changing to different packages for non-ophthalmic products in plastic bottles mimicking eyedroppers is essential to minimize these injuries. Full article

Shale pore architecture governs gas storage capacity, permeability, and production potential in reservoirs. Therefore, this study systematically investigates the pore structure features and influencing factors of the Niutitang Formation shale from the QX1 well in northern Guizhou using field emission scanning electron microscopy (FE-SEM), high-pressure mercury intrusion (HPMI), low-temperature nitrogen adsorption (LTNA), and nuclear magnetic resonance (NMR) experiments. The results show that ① The pore size of the QX1 well’s Niutitang Formation shale is primarily in the nanometer range, with pore types including intragranular pores, intergranular pores, organic matter pores, and microfractures, with the former two types constituting the primary pore network. ② Pore shapes are plate-shaped intersecting conical microfractures or plate-shaped intersecting ink bottles, ellipsoidal, and beaded pores. ③ The pore size distribution showed a multi-peak distribution, predominantly mesopores, followed by micropores, with the fewest macropores. ④ The fractal dimension D₁ > D₂ indicates that the shale pore system is characterized by a rough surface and some connectivity of the pore network. ⑤ Carbonate mineral abundances are the main controlling factors affecting the pore structure of shales in the study area, and total organic carbon (TOC) content also has some influence, while clay mineral content shows negligible statistical correlation. Full article

The development of deep coalbed methane (buried depth > 2000 m) in the Yan’an block of Ordos Basin is limited by low permeability, the pore structure of the coal reservoir, and the gas–water occurrence relationship. It is urgent to clarify the key control mechanism of pore structure on gas migration. In this study, based on high-pressure mercury intrusion (pore size > 50 nm), low-temperature N₂/CO₂ adsorption (0.38–50 nm), low-field nuclear magnetic resonance technology, fractal theory and Pearson correlation coefficient analysis, quantitative characterization of multi-scale pore–fluid system was carried out. The results show that the multi-scale pore network in the study area jointly regulates the occurrence and migration process of deep coalbed methane in Yan’an through the ternary hierarchical gas control mechanism of ‘micropore adsorption dominant, mesopore diffusion connection and macroporous seepage bottleneck’. The fractal dimensions of micropores and seepage are between 2.17–2.29 and 2.46–2.58, respectively. The shape of micropores is relatively regular, the complexity of micropore structure is low, and the confined space is mainly slit-like or ink bottle-like. The pore-throat network structure is relatively homogeneous, the difference in pore throat size is reduced, and the seepage pore shape is simple. The bimodal structure of low-field nuclear magnetic resonance shows that the bound fluid is related to the development of micropores, and the fluid mobility mainly depends on the seepage pores. Pearson’s correlation coefficient showed that the specific surface area of micropores was strongly positively correlated with methane adsorption capacity, and the nanoscale pore-size dominated gas occurrence through van der Waals force physical adsorption. The specific surface area of mesopores is significantly positively correlated with the tortuosity. The roughness and branch structure of the inner surface of the channel lead to the extension of the migration path and the inhibition of methane diffusion efficiency. Seepage porosity is linearly correlated with gas permeability, and the scale of connected seepage pores dominates the seepage capacity of reservoirs. This study reveals the pore structure and ternary grading synergistic gas control mechanism of deep coal reservoirs in the Yan’an Block, which provides a theoretical basis for the development of deep coalbed methane. Full article

The complexity of the pore system hindered our understanding of the storage and transport properties of organic-rich shales, which in turn brought challenges to the efficient exploration and development of shale oil and gas. This study, based on elemental, mineralogical, petrographic, and petrophysical approaches, attempts to reveal the pore structure and fractal characteristics of a suite of Permian shales collected from the northeastern Sichuan Basin, China. The results showed that meso-pores make up the main proportion of the total pore volume in the Permian shale in this study; most of the pore size distribution patterns for micro pores and meso-macropores are bimodal. Pores related to clay minerals, organic matter pores, and intragranular dissolution pores are the main storage spaces in these shales. In these samples, ink-bottle pores dominate, with some slit and wedge-shaped ones developed. The morphology of the pores in the studied shales is mainly ink-bottle pores, with some slit-shaped and wedge-shaped pores. The fractal dimension D₂ is greater than D₁, indicating that the homogeneity of pore space is stronger than that of the specific surface area. Quartz in Permian shales inhibits the development of macro- and mesopore spaces and enhances pore heterogeneity, while clay minerals facilitate the development of macro- and mesopore spaces and attenuate pore heterogeneity. The organic matter content shows a negative impact on the macropore volume due to the stripped occurrence and matrix filling. This study has a vital significance for current exploration and development of shale gas in Permian strata in the Sichuan Basin and offers insights for Permian shales in other basins all over the world. Full article

A cheap, environmentally friendly, easily scalable post-treatment of Na-ZSM-5 (Si/Al molar ratio = 20 or 30) via electron-beam irradiation to produce hierarchical H-ZSM-5 as a propylene-increasing fluid catalytic cracking additive was performed. Higher specific surface areas and highly accessible porous systems were obtained among the irradiated samples. A combination of ²⁷Al, ¹H magic angle spinning nuclear magnetic resonance and NH₃-temperature-programmed desorption methods showed that upon irradiation, some of the framework’s tetrahedral Al atoms were removed as non-framework Al atoms via flexible coordination with Si-OH groups (either framework or non-framework defects), thus increasing the H-ZSM-5 acidity and stability during hydrothermal dealumination. The enhanced selectivity and stability toward propylene production over the irradiated H-ZSM-5 samples were attributed to the integration of the reserved population of medium acid sites into the highly accessible hierarchical network. N₂ adsorption–desorption isotherm data showed that the Si-rich H-ZSM-5 samples possessed an obvious ink-bottle-shaped micro-mesopore network and a greater degree of disordered orientation of the straight pore systems toward the exterior surfaces. Micro-activity test data suggested that with an increasing Si/Al ratio, the H-ZSM-5 additives lost some extent of their cracking activity due to the constricted hierarchical pore network toward the exterior surface but gained more stability and selectivity for propylene due to the reserved medium acid sites. Full article

In order to better understand the micropore structure of shale reservoir in Jiyang Depression, permeability damage test, low temperature nitrogen adsorption and scanning electron microscopy (SEM) were carried out on six cores in the target block. The adsorption isotherms were analyzed by Frenkel–Halsey–Hill (FHH) model, and the fractal dimensions of different layers were calculated. The results show that the shale pore system is mainly composed of organic nanopores, inorganic nanopores and micro-fractures. The inorganic pores are mainly distributed around or inside the mineral particles, while microcracks are commonly found between mineral particles or at the organic–mineral interface. Organic pores are located within or between organic particles. The results of nitrogen adsorption show that the shale pores are mainly H2/H3 hysteresis loops with wedge, plate or ink bottle shapes. The pore structure is highly complex, and the fractal dimension is high. The mean D1 fractal dimension, which represents pore surface roughness, is 2.3788, and the mean D2 fractal dimension, which represents pore structure complexity, is 2.7189. The fractal dimension is positively correlated with specific surface area and total pore volume and negatively correlated with average pore radius. The permeability damage rates of the N layer, B layer, and F layer are 17.39%, 20.2%, and 21.6%, respectively. The contact Angle of the core decreases with the increase in water skiing time. In this study, the micropore structure of different formations in Jiyang Depression is compared and analyzed, which provides valuable insights for the optimization and differentiated development of shale oil and gas resources. Full article

The dosage of mineral powder has a complex influence on the compressive strength of self-compacting concrete, among which the pore structure is a key determining factor. This study investigated the effects of different dosages of mineral powder (0%, 5%, 10%, 20%, and 30%) on the workability, mechanical properties, and pore distribution in C80 self-compacting concrete. The research results show that an appropriate dosage of mineral powder (0–20%) can significantly improve the spreadability and fluidity of C80 self-compacting concrete. This phenomenon is mainly attributed to the shape effect and micro-aggregate effect of mineral powder, which improve the fluidity of concrete, reduce the viscosity of the paste, and thereby increase the spreadability and gap-passing rate. By testing the BSD-PS1/2 series fully automatic specific surface area and pore size analyzer, we found that there are columnar pores and ink bottle-shaped pores in C80 self-compacting concrete, as well as a small amount of plate-like slit structures. Our observations with an SEM scanning electron microscope revealed that the width of micro-cracks and micro-holes is between 1 and 5 μm and the diameter is between 3 and 10 μm. These microstructures may have an impact on the mechanical properties of the structure. By applying fractal theory and low-temperature liquid nitrogen adsorption tests, this study revealed the relationship between the fractal characteristics of internal pores in C80 self-compacting concrete and the dosage of mineral powder. The results show that with the increase in mineral powder dosage, the fractal dimension first decreases and then increases, reflecting the change rule of the complexity of pore structure first decreasing and then increasing. When the dosage of mineral powder is about 20%, the compressive strength of SCC reaches the maximum value, and this dosage range should be considered in engineering design. Full article

Heterogeneity of pore and fracture structures has become an important factor affecting the migration of methane and water in coal reservoirs. However, controlling factors of pore and fracture structure collected from coal seam 10 in Taliqike Formation, Kubai Coalfield, Xinjiang need to be studied. In this paper, carbon dioxide adsorption, cryogenic liquid nitrogen, and high-pressure mercury intrusion, as well as coal microscopic components, were used to study pore volumes and characterize pore diameter distribution heterogeneity. By the theory of single weight and multiple fractal formations, the heterogeneity of the pore fracture structure of coal reservoir is expressed, and the influencing factors of the heterogeneity of the pore fracture structure and the pore volume are also discussed. The results are as follows. (1) Micro-pore distribution presents a distinct bidirectional state, with the main peak at approximately 0.6 nm and 0.85 nm. R_o,max has an obvious influence on micro-pore volume. The single-fractal dimension of micro-pore is not affected by a micro-pore volume but is influenced by other factors such as R_o,max and microscopic composition. The heterogeneity of the low-value area controls the heterogeneity of micro-pore diameter distribution. (2) For lower R_o,max samples, mesopores of these samples are ink bottle-shaped pores, and the pore connectivity is poor. In contrast, meso-pore of higher thermal evolution coal samples are mostly simple pores, such as parallel plates. The main mesopores are 10–100 nm pores, accounting for 75% of the total meso-pore volume. For the single fractal dimension, D₁ is greater than D₂, which also shows that the heterogeneity of a pore structure greater than 4 nm is much stronger than that of a pore structure less than 4 nm in these samples. (3) For lower R_o,max samples, double S-shaped curves with distinct hysteresis loop are obtained, while samples of higher R_o,max samples show parallel curves, suggesting that macro-pore of this type of sample develops parallel plate-like pore. There is a positive relationship between D₋₁₀–D₀ and D₋₁₀–D₁₀, while D₀–D₁₀ and D₋₁₀–D₀ have a weak correlation. With the increase of 2–10 nm pore volume, pore distribution heterogeneity of lower value area (D₋₁₀–D₀) weakens. This indicates that pore volume is an important factor affecting the multifractal variation. Full article

The geological structure of interbedded shale oil reservoirs is complex, later characterized by high reservoir heterogeneity and diverse reservoir spaces. These distinctive features are primarily attributed to their unique source–storage configuration. This paper comprehensively investigates the pore structure characteristics and controlling factors, which are beneficial for realizing efficient and sustainable resource utilization. The pore structure characteristics and main control factors of interbedded shale oil in the Heshuinan (HSN) area of the Ordos Basin are studied by analyzing thin sections and scanning them under an electron microscope, and using XRD analysis, a high-pressure mercury injection, a constant-rate mercury injection, and a nitrogen adsorption method. The influence of sedimentation and diagenesis on the pore structure is analyzed. Research shows that the interbedded shale oil reservoirs of the Triassic Chang 7 in the HSN area have an average porosity of 8.47% and an average permeability of 0.74 × 10⁻³ μm². The reservoirs are classified as typical ultra-low porosity, ultra-low permeability reservoirs. The various pore types in the study area are mainly residual intergranular pores and feldspar dissolution pores. The pores are mostly in the shape of parallel slits and ink-bottle-shaped. The pore-throat radii range from 0.02 μm to 200 μm. Sedimentation and diagenesis jointly control the pore structure in the study area. Sedimentation determines the material foundation of the study area. Diagenesis affects later pore development. Early compaction greatly reduces the intergranular pores, but the chlorite envelope reduces the influence of compaction to some extent. The compacted residual intergranular pores are further reduced by clay minerals, carbonate minerals, and siliceous minerals. Late dissolution promotes pore enlargement, which is the key to the formation of high-quality reservoirs. Furthermore, on this basis, this paper outlines the genetic mechanism of the Chang 7 high-quality reservoir in the HSN area to provide guidance for the exploration and development of interbedded shale oil and gas. Full article

Carbon dioxide (CO₂) storage in sandstones is vital for enhancing oil/gas recovery and reducing CO₂ emissions. The introduction of CO₂ into sandstone reservoirs leads to chemical reactions between CO₂ and minerals present in sandstone, which changes the pore structure of the sandstone reservoir. Herein, tight sandstone samples from the Coal-Measure Strata of the Shanxi Formation in the Huxiang area, Henan Province, were selected for simulation in this experimental study under supercritical CO₂ (ScCO₂)–H₂O treatment in reservoir conditions. Further, mercury intrusion porosimetry and low-pressure nitrogen adsorption/desorption methods were used to analyze the evolution of the pore structures of tight sandstones, and the mechanism of pore structure evolution was discussed. The results show that pore volumes and specific surface areas in the micropores and transitional pores decreased after the ScCO₂–H₂O treatment, while those in the mesopores and macropores increased. In the micropores and transitional pores, some of the pores changed from open pores and ink-bottle-shaped pores to semi-closed pores after the ScCO₂–H₂O treatment, and the pore morphology became narrower, which might have deteriorated the pore connectivity. A pore structure evolution model of ScCO₂–H₂O-treated tight sandstones was proposed. The evolution of pore structure is a result of the synergistic effect of pore enlargement caused by mineral dissolution and secondary mineral precipitation, which together play a controlling role in pore structure evolution. This study is conducive to understanding the pore structure evolution under ScCO₂–H₂O treatment and implementing CO₂ storage and enhancing oil/gas recovery in sandstone reservoirs. Full article

The mechanical properties of High-Performance Self-Compacting Concrete (HPSCC) are strongly influenced by its pore structure, but the impact of varying water–binder ratios (W/C) on this relationship remains unclear. To address this, the present study investigates HPSCC with W/C ratios ranging from 0.19 to 0.23, aiming to elucidate the connection between pore structure, fractal characteristics, and mechanical performance. Through a combination of compressive strength testing, low-temperature nitrogen adsorption, and Scanning Electron Microscopy (SEM) observations, this study reveals key insights. First, compressive strength initially increases with a decreasing W/C ratio but plateaus beyond W/C = 0.21, identifying an optimal range for balancing strength and workability. Second, the pore structure of HPSCC is characterized by cylindrical, ink-bottle, and planar interstitial pores, with significant fractal characteristics. Notably, the fractal dimension decreases as the W/C ratio increases, indicating reduced pore complexity and improved homogeneity. Finally, a strong linear correlation (R² > 0.9) between the W/C ratio, fractal dimension, and compressive strength provides a predictive tool for assessing HPSCC performance. This study concludes that the internal pore structure is a critical determinant of HPSCC strength, and the identified optimal W/C ratio range offers guidance for mixture designs. Additionally, fractal dimension analysis emerges as a novel method to evaluate HPSCC’s microstructural quality, enabling predictions of long-term performance and durability. These findings contribute to the scientific basis for designing high-performance concrete materials with improved mechanical properties and durability. Full article

With the evolution of unconventional oil and gas exploration concepts from source rocks and reservoirs to carrier beds, the inter-layer sandstone carrier bed within marine–continental transitional shale strata has emerged as a significant target for oil and gas exploration. The inter-layer sandstone is closely associated with the source rock and differs from conventional tight sandstone in terms of sedimentary environment, matrix composition, and the characteristics of reservoir microscopic pore development. Preliminary exploration achievements display that the inter-layer sandstone is plentiful in gas content and holds promising prospects for exploration and development. Consequently, it is essential to investigate the gas-rich accumulation theory specific to the inter-layer sandstone reservoir in transitional facies. Pore development characteristics and heterogeneity are crucial aspects of oil and gas accumulation research, as they influence reservoir seepage performance and capacity. This paper employs total organic carbon analysis, X-ray diffraction, rock thin section examination, field emission scanning electron microscopy, physical analysis, high-pressure mercury intrusion analysis, gas adsorption experiments, and fractal theory to explore the reservoir development characteristics of the sandstone samples from the Upper Permian marine–continental transitional facies Longtan Formation in the southern Sichuan Basin. It also attempts to combine high-pressure mercury intrusion analysis and gas adsorption experiments to describe the structural and fractal characteristics of pores at different scales in a segmented manner. The findings reveal that the sandstone type of the Longtan Formation is mainly lithic sandstone. The pore size distribution of the sandstone primarily falls below 30 nm and above 1000 nm, with the main pore types being inter-granular pores and micro-fractures in clay minerals. The pore volume and specific surface area are largely attributed to the micropores and mesopores of clay minerals. The pore morphology is complex, exhibiting strong heterogeneity, predominantly characterized by slit-like and ink bottle-like features. Notably, there are discernible differences in reservoir structural characteristics and homogeneity between muddy sandstone and non-muddy sandstone. The pore morphology is complex, exhibiting strong heterogeneity, predominantly characterized by slit-like and ink bottle-like features. Notably, there are discernible differences in reservoir structural characteristics and homogeneity between muddy sandstone and non-muddy sandstone. Full article

Shale gas is a prospective cleaner energy resource and the exploration and development of shale gas has made breakthroughs in many countries. Structure deformation is one of the main controlling factors of shale gas accumulation and enrichment in complex tectonic areas in southern China. In order to estimate the shale gas capacity of structurally deformed shale reservoirs, it is necessary to understand the systematic evolution of organic pores in the process of structural deformation. In particular, as the main storage space of high-over-mature marine shale reservoirs, the organic matter pore system directly affects the occurrence and migration of shale gas; however, there is a lack of systematic research on the fractal characteristics and deformation mechanism of organic pores under the background of different tectonic stresses. Therefore, to clarify the above issues, modular automated processing system (MAPS) scanning, low-pressure gas adsorption, quantitative evaluation of minerals by scanning (QEMSCAN), and focused ion beam scanning electron microscopy (FIB-SEM) were performed and interpreted with fractal and morphology analyses to investigate the deformation mechanisms and structure of organic pores from different tectonic units in Silurian Longmaxi shale. Results showed that in stress concentration areas such as around veins or high-angle fractures, the organic pore length-width ratio and the fractal dimension are higher, indicating that the pore is more obviously modified by stress. Under different tectonic backgrounds, the shale reservoir in Weiyuan suffered severe denudation and stronger tectonic compression during burial, which means that the organic pores are dominated by long strip pores and slit-shaped pores with high fractal dimension, while the pressure coefficient in Luzhou is high and the structural compression is weak, resulting in suborbicular pores and ink bottle pores with low fractal dimension. The porosity and permeability of different forms of organic pores are also obviously different; the connectivity of honeycomb pores with the smallest fractal dimension is the worst, that of suborbicular organic pores is medium, and that of long strip organic pores with the highest fractal dimension is the best. This study provides more mechanism discussion and case analysis for the microscopic heterogeneity of organic pores in shale reservoirs and also provides a new analysis perspective for the mechanism of shale gas productivity differences in different stress–strain environments. Full article