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18 pages, 2436 KiB  
Article
Leveraging IGOOSE-XGBoost for the Early Detection of Subclinical Mastitis in Dairy Cows
by Rui Guo and Yongqiang Dai
Appl. Sci. 2025, 15(15), 8763; https://doi.org/10.3390/app15158763 (registering DOI) - 7 Aug 2025
Abstract
Subclinical mastitis in dairy cows poses a significant challenge to the dairy industry, leading to reduced milk yield, altered milk composition, compromised animal health, and substantial economic losses for dairy farmers. A model based on the XGBoost algorithm, optimized with an Improved GOOSE [...] Read more.
Subclinical mastitis in dairy cows poses a significant challenge to the dairy industry, leading to reduced milk yield, altered milk composition, compromised animal health, and substantial economic losses for dairy farmers. A model based on the XGBoost algorithm, optimized with an Improved GOOSE Optimization Algorithm (IGOOSE), is presented in this work as an innovative approach for predicting subclinical mastitis in order to overcome these problems. The Dairy Herd Improvement (DHI) records of 4154 cows served as the model’s original foundation. A total of 3232 samples with 21 characteristics made up the final dataset, following extensive data cleaning and preprocessing. To overcome the shortcomings of the original GOOSE algorithm in intricate, high-dimensional problem spaces, three significant enhancements were made. First, an elite inverse strategy was implemented to improve population initialization, enhancing the algorithm’s balance between global exploration and local exploitation. Second, an adaptive nonlinear control factor was added to increase the algorithm’s stability and convergence speed. Lastly, a golden sine strategy was adopted to reduce the risk of premature convergence to suboptimal solutions. According to experimental results, the IGOOSE-XGBoost model works better than other models in predicting subclinical mastitis, especially when it comes to recognizing somatic cell scores, which are important markers of the illness. This study provides a strong predictive framework for managing the health of dairy cows, allowing for the prompt identification and treatment of subclinical mastitis, which enhances the efficiency and quality of milk supply. Full article
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26 pages, 674 KiB  
Article
Toward Standardised Construction Pipeline Data: Conceptual Minimum Dataset Framework
by Elrasheid Elkhidir, James Olabode Bamidele Rotimi, Tirth Patel, Taofeeq D. Moshood and Suzanne Wilkinson
Buildings 2025, 15(15), 2797; https://doi.org/10.3390/buildings15152797 (registering DOI) - 7 Aug 2025
Abstract
The construction industry is a cornerstone of New Zealand (NZ)’s economic growth, yet strategic infrastructure planning is constrained by fragmented and inconsistent pipeline data. Despite the increasing availability of construction pipeline datasets in NZ, their limited clarity, interoperability, and standardisation impede effective forecasting, [...] Read more.
The construction industry is a cornerstone of New Zealand (NZ)’s economic growth, yet strategic infrastructure planning is constrained by fragmented and inconsistent pipeline data. Despite the increasing availability of construction pipeline datasets in NZ, their limited clarity, interoperability, and standardisation impede effective forecasting, policy development, and investment alignment. These challenges are compounded by disparate data structures, inconsistent reporting formats, and semantic discrepancies across sources, undermining cross-agency coordination and long-term infrastructure governance. To address this issue, the study begins by assessing the quality of four prominent pipeline datasets using Wang and Strong’s multidimensional data quality framework. This evaluation provides a necessary foundation for identifying the structural and semantic barriers that limit data integration and informed decision-making. The analysis examines four dimensions of data quality: accessibility, intrinsic quality, contextual relevance, and representational clarity. The findings reveal considerable inconsistencies in data fields, classification systems, and levels of detail across the datasets. Building on these insights, this study also develops a conceptual minimum dataset (MDS) framework comprising three core thematic categories: project identification, project characteristics, and project budget and timing. The proposed conceptual MDS includes unified data definitions, standardised reporting formats, and semantic alignment to enhance cross-platform usability and data confidence. This framework applies to the New Zealand context and is designed for replication in other jurisdictions, supporting the global push toward open, high-quality infrastructure data. The study contributes to the construction informatics and infrastructure planning by offering a practical solution to a critical data governance issue and introducing a transferable methodology for developing minimum data standards in the built environment to enable more informed, coordinated, and evidence-based decision-making. Full article
(This article belongs to the Special Issue Big Data and Machine/Deep Learning in Construction)
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21 pages, 4368 KiB  
Article
Damage Mechanism Characterization of Glass Fiber-Reinforced Polymer Composites: A Study Using Acoustic Emission Technique and Unsupervised Machine Learning Algorithms
by Jorge Palacios Moreno, Hadi Nazaripoor and Pierre Mertiny
J. Compos. Sci. 2025, 9(8), 426; https://doi.org/10.3390/jcs9080426 - 7 Aug 2025
Abstract
Recent advancements in composite materials design have made glass fiber-reinforced polymer composites (GFRPC) a viable choice for a wide range of engineering and industrial applications. Although GFRPCs boast attractive characteristics such as low specific mass and high specific mechanical strength, identifying and characterizing [...] Read more.
Recent advancements in composite materials design have made glass fiber-reinforced polymer composites (GFRPC) a viable choice for a wide range of engineering and industrial applications. Although GFRPCs boast attractive characteristics such as low specific mass and high specific mechanical strength, identifying and characterizing damage mechanisms in these materials is challenging. Several scientific studies have examined the root causes of GFRPC failure using various methods, including non-destructive techniques and learning algorithms. Despite this, ongoing investigations aim to accurately detect mechanical defects in GFRPCs. This study explores the use of non-destructive testing (NDT) combined with unsupervised learning algorithms to identify and classify damage mechanisms in GFRPCs. The NDT method employed in this study is acoustic emission (AE), which identifies waveforms associated with various failure mechanisms during testing. These waveforms are categorized using unsupervised learning methods such as principal component analysis (PCA) and self-organizing maps. PCA selects the most appropriate AE descriptors for distinguishing between different damage mechanisms, while the self-organizing maps algorithm performs clustering analysis and classifies failure mechanisms. Scanning electron microscope images of the observed failures are provided to sup-port the findings derived from AE data. Full article
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21 pages, 2090 KiB  
Article
The Dynamic Evolution of Industrial Electricity Consumption Linkages and Flow Path in China
by Jinshi Wei
Energies 2025, 18(15), 4203; https://doi.org/10.3390/en18154203 - 7 Aug 2025
Abstract
An in-depth investigation into the evolutionary characteristics, transmission mechanisms, and optimization pathways of electricity consumption linkages across China’s industrial sectors highlights their substantial theoretical and practical significance in achieving the “dual carbon” goals and advancing high-quality economic development. This study investigates the structural [...] Read more.
An in-depth investigation into the evolutionary characteristics, transmission mechanisms, and optimization pathways of electricity consumption linkages across China’s industrial sectors highlights their substantial theoretical and practical significance in achieving the “dual carbon” goals and advancing high-quality economic development. This study investigates the structural characteristics and developmental trends of electricity consumption linkages across China’s industrial sectors using an enhanced hypothetical extraction method. The analysis draws on national input–output tables and sector-specific electricity consumption data during the period from 2002 to 2020. Key transmission routes between industrial sectors are identified through path analysis and average path length calculations. The findings reveal that China’s industrial electricity consumption structure is marked by notable scale expansion and differentiation. The magnitude of inter-sectoral electricity flows continues to grow steadily. The evolution of these linkages exhibits clear phase-specific patterns, while the intensity of electricity consumption connections across sectors shows pronounced heterogeneity. Furthermore, the transmission path analysis revealed differentiated characteristics of electricity influence transmission, with generally shorter internal paths within sectors, significant cross-sectoral transmission differences, and manufacturing demonstrating good transmission accessibility with moderate path distances to major sectors. These insights provide a robust foundation for designing differentiated energy conservation policies, as well as for optimizing the overall structure of industrial electricity consumption. Full article
(This article belongs to the Special Issue Sustainable Energy Futures: Economic Policies and Market Trends)
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19 pages, 371 KiB  
Review
Human Breast Milk as a Biological Matrix for Assessing Maternal and Environmental Exposure to Dioxins and Dioxin-like Polychlorinated Biphenyls: A Narrative Review of Determinants
by Artemisia Kokkinari, Evangelia Antoniou, Kleanthi Gourounti, Maria Dagla, Aikaterini Lykeridou, Stefanos Zervoudis, Eirini Tomara and Georgios Iatrakis
Pollutants 2025, 5(3), 25; https://doi.org/10.3390/pollutants5030025 - 7 Aug 2025
Abstract
(1) Background: Dioxins and dioxin-like polychlorinated biphenyls (dl-PCBs) are persistent organic pollutants (POPs), characterized by high toxicity and strong lipophilicity, which promote their bioaccumulation in human tissues. Their detection in breast milk raises concerns about early-life exposure during lactation. Although dietary intake is [...] Read more.
(1) Background: Dioxins and dioxin-like polychlorinated biphenyls (dl-PCBs) are persistent organic pollutants (POPs), characterized by high toxicity and strong lipophilicity, which promote their bioaccumulation in human tissues. Their detection in breast milk raises concerns about early-life exposure during lactation. Although dietary intake is the primary route of maternal exposure, environmental pathways—including inhalation, dermal absorption, and residential proximity to contaminated sites—may also significantly contribute to the maternal body burden. (2) Methods: This narrative review examined peer-reviewed studies investigating maternal and environmental determinants of dioxin and dl-PCB concentrations in human breast milk. A comprehensive literature search was conducted in PubMed, Scopus, and Web of Science (2000–2024), identifying a total of 325 records. Following eligibility screening and full-text assessment, 20 studies met the inclusion criteria. (3) Results: The included studies consistently identified key exposure determinants, such as high consumption of animal-based foods (e.g., meat, fish, dairy), living near industrial facilities or waste sites, and maternal characteristics including age, parity, and body mass index (BMI). Substantial geographic variability was observed, with higher concentrations reported in regions affected by industrial activity, military pollution, or inadequate waste management. One longitudinal study from Japan demonstrated a declining trend in dioxin levels in breast milk, suggesting the potential effectiveness of regulatory interventions. (4) Conclusions: These findings highlight that maternal exposure to dioxins is influenced by identifiable environmental and behavioral factors, which can be mitigated through public health policies, targeted dietary guidance, and environmental remediation. Breast milk remains a critical bioindicator of human exposure. Harmonized, long-term research is needed to clarify health implications and minimize contaminant transfer to infants, particularly among vulnerable populations. Full article
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25 pages, 2458 KiB  
Article
Numerical Analysis of Heat Transfer in a Double-Pipe Heat Exchanger for an LPG Fuel Supply System
by Seongwoo Lee, Younghun Kim, Ancheol Choi and Sungwoong Choi
Energies 2025, 18(15), 4179; https://doi.org/10.3390/en18154179 - 6 Aug 2025
Abstract
LPG fuel supply systems are increasingly important for improving energy efficiency and reducing carbon emissions in the shipping industry. The primary objective of this research is to investigate the heat transfer phenomena to enhance the thermal performance of double-pipe heat exchangers (DPHEs) in [...] Read more.
LPG fuel supply systems are increasingly important for improving energy efficiency and reducing carbon emissions in the shipping industry. The primary objective of this research is to investigate the heat transfer phenomena to enhance the thermal performance of double-pipe heat exchangers (DPHEs) in LPG fuel supply systems. This study investigates the heat transfer performance of a glycol–steam double-pipe heat exchanger (DPHE) within an LPG fuel supply system under varying operating conditions. A computational model and methodology were developed and validated by comparing the numerical results with experimental data obtained from commissioning tests. Additionally, the effects of turbulence models and parametric variations were evaluated by analyzing the glycol–water mixing ratio and flow direction—both of which are critical operational parameters for DPHE systems. Numerical validation against the commissioning data showed a deviation of ±2% under parallel-flow conditions, confirming the reliability of the proposed model. With respect to the glycol–water mixing ratio and flow configuration, thermal conductance (UA) decreased by approximately 11% in parallel flow and 13% in counter flow for every 20% increase in glycol concentration. Furthermore, parallel flow exhibited approximately 0.6% higher outlet temperatures than counter flow, indicating superior heat transfer efficiency under parallel-flow conditions. Finally, the heat transfer behavior of the DPHE was further examined by considering the effects of geometric characteristics, pipe material, and fluid properties. This study offers significant contributions to the engineering design of double-pipe heat exchanger systems for LPG fuel supply applications. Full article
(This article belongs to the Collection Advances in Heat Transfer Enhancement)
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22 pages, 3079 KiB  
Review
Progress in Caking Mechanism and Regulation Technologies of Weakly Caking Coal
by Zhaoyang Li, Shujun Zhu, Ziqu Ouyang, Zhiping Zhu and Qinggang Lyu
Energies 2025, 18(15), 4178; https://doi.org/10.3390/en18154178 - 6 Aug 2025
Abstract
Efficient and clean utilization remains a pivotal development focus within the coal industry. Nevertheless, the application of weakly caking coal results in energy loss due to the caking property, thereby leading to a waste of resources. This paper, therefore, concentrates on the caking [...] Read more.
Efficient and clean utilization remains a pivotal development focus within the coal industry. Nevertheless, the application of weakly caking coal results in energy loss due to the caking property, thereby leading to a waste of resources. This paper, therefore, concentrates on the caking property, offering insights into the relevant caking mechanism, evaluation indexes, and regulation technologies associated with it. The caking mechanism delineates the transformation process of coal into coke. During pyrolysis, the active component generates the plastic mass in which gas, liquid, and solid phases coexist. With an increase in temperature, the liquid phase is diminished gradually, causing the inert components to bond. Based on the caking mechanism, evaluation indexes such as that characteristic of char residue, the caking index, and the maximal thickness of the plastic layer are proposed. These indexes are used to distinguish the strength of the caking property. However, they frequently exhibit a poor differentiation ability and high subjectivity. Additionally, some technologies have been demonstrated to regulate the caking property. Technologies such as rapid heating treatment and hydrogenation modification increase the amount of plastic mass generated, thereby improving the caking property. Meanwhile, technologies such as mechanical breaking and pre-oxidation reduce the caking property by destroying agglomerates or consuming plastic mass. Full article
(This article belongs to the Special Issue Advanced Clean Coal Technology)
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22 pages, 1177 KiB  
Article
An Empirical Study on the Impact of Financial Technology on the Profitability of China’s Listed Commercial Banks
by Xue Yuan, Chin-Hong Puah and Dayang Affizzah binti Awang Marikan
J. Risk Financial Manag. 2025, 18(8), 440; https://doi.org/10.3390/jrfm18080440 - 6 Aug 2025
Abstract
This paper selects 50 listed commercial banks in China from 2012 to 2023 as research samples, and employs the fixed effects model and Hansen’s threshold regression method to systematically examine the impact mechanism and non-linear characteristics of FinTech development on the profitability of [...] Read more.
This paper selects 50 listed commercial banks in China from 2012 to 2023 as research samples, and employs the fixed effects model and Hansen’s threshold regression method to systematically examine the impact mechanism and non-linear characteristics of FinTech development on the profitability of commercial banks. The key findings are summarized as follows: (1) FinTech significantly undermines the overall profitability of commercial banks by reshaping the competitive landscape of the industry and intensifying the technology substitution effect. This is primarily reflected in the reduction in traditional interest income and the erosion of market share in intermediary business. (2) Heterogeneity analysis indicates that large state-owned banks and joint-stock banks experience more pronounced negative impacts compared to small and medium-sized banks. (3) Additional research findings reveal a significant single-threshold effect between FinTech and bank profitability, with a critical value of 4.169. When the development level of FinTech surpasses this threshold, its inhibitory effect diminishes substantially, suggesting that after achieving a certain degree of technological integration, commercial banks may partially alleviate external competitive pressures through synergistic effects. This study offers crucial empirical evidence and theoretical support for commercial banks to develop differentiated technology strategies and for regulatory authorities to design dynamically adaptable policy frameworks. Full article
(This article belongs to the Section Financial Technology and Innovation)
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14 pages, 7543 KiB  
Article
Production of Transgenic Silkworm Using Anti-Serum Against Diapause Hormone in Diapause Strains of Silkworm, Bombyx mori
by Keiro Uchino, Megumi Sumitani, Tetsuya Iizuka and Hideki Sezutsu
Int. J. Mol. Sci. 2025, 26(15), 7604; https://doi.org/10.3390/ijms26157604 - 6 Aug 2025
Abstract
In general, the silkworm, Bombyx mori, has a diapause trait in its eggs. Therefore, transgenic silkworm can be produced by embryonic microinjection using eggs laid by a non-diapause strain in B. mori. In this study, we performed microinjection using eggs of diapause [...] Read more.
In general, the silkworm, Bombyx mori, has a diapause trait in its eggs. Therefore, transgenic silkworm can be produced by embryonic microinjection using eggs laid by a non-diapause strain in B. mori. In this study, we performed microinjection using eggs of diapause strains which have good characteristics for industrial use, such as a big cocoon, thin and smooth silk, and tolerance against disease due to the growing industrial use of transgenic silkworms. For the conversion of egg diapause traits from diapause to non-diapause types, we used anti-serum against the diapause hormone of B. mori (BmDH), which was injected into maternal pupae, producing non-diapause eggs at a high rate. Finally, we attempted microinjection using three diapause strains with different voltinism (i.e., number of generations of an organism in a year) and were able to successfully produce transgenic silkworms in all three of them, demonstrating that our method is applicable to a wide range of silkworm strains with a diapause trait. Full article
(This article belongs to the Collection Feature Papers in “Molecular Biology”)
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30 pages, 8483 KiB  
Article
Research on Innovative Design of Two-in-One Portable Electric Scooter Based on Integrated Industrial Design Method
by Yang Zhang, Xiaopu Jiang, Shifan Niu and Yi Zhang
Sustainability 2025, 17(15), 7121; https://doi.org/10.3390/su17157121 - 6 Aug 2025
Abstract
With the advancement of low-carbon and sustainable development initiatives, electric scooters, recognized as essential transportation tools and leisure products, have gained significant popularity, particularly among young people. However, the current electric scooter market is plagued by severe product similarity. Once the initial novelty [...] Read more.
With the advancement of low-carbon and sustainable development initiatives, electric scooters, recognized as essential transportation tools and leisure products, have gained significant popularity, particularly among young people. However, the current electric scooter market is plagued by severe product similarity. Once the initial novelty fades for users, the usage frequency declines, resulting in considerable resource wastage. This research collected user needs via surveys and employed the KJ method (affinity diagram) to synthesize fragmented insights into cohesive thematic clusters. Subsequently, a hierarchical needs model for electric scooters was constructed using analytical hierarchy process (AHP) principles, enabling systematic prioritization of user requirements through multi-criteria evaluation. By establishing a house of quality (HoQ), user needs were transformed into technical characteristics of electric scooter products, and the corresponding weights were calculated. After analyzing the positive and negative correlation degrees of the technical characteristic indicators, it was found that there are technical contradictions between functional zoning and compact size, lightweight design and material structure, and smart interaction and usability. Then, based on the theory of inventive problem solving (TRIZ), the contradictions were classified, and corresponding problem-solving principles were identified to achieve a multi-functional innovative design for electric scooters. This research, leveraging a systematic industrial design analysis framework, identified critical pain points among electric scooter users, established hierarchical user needs through priority ranking, and improved product lifecycle sustainability. It offers novel methodologies and perspectives for advancing theoretical research and design practices in the electric scooter domain. Full article
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20 pages, 772 KiB  
Review
Treatment of Refractory Oxidized Nickel Ores (ONOs) from the Shevchenkovskoye Ore Deposit
by Chingis A. Tauakelov, Berik S. Rakhimbayev, Aliya Yskak, Khusain Kh. Valiev, Yerbulat A. Tastanov, Marat K. Ibrayev, Alexander G. Bulaev, Sevara A. Daribayeva, Karina A. Kazbekova and Aidos A. Joldassov
Metals 2025, 15(8), 876; https://doi.org/10.3390/met15080876 - 6 Aug 2025
Abstract
The increasing depletion of high-grade nickel sulfide deposits and the growing demand for nickel have intensified global interest in oxidized nickel ores (ONOs), particularly those located in Kazakhstan. This study presents a comprehensive review of the mineralogical and chemical characteristics of ONOs from [...] Read more.
The increasing depletion of high-grade nickel sulfide deposits and the growing demand for nickel have intensified global interest in oxidized nickel ores (ONOs), particularly those located in Kazakhstan. This study presents a comprehensive review of the mineralogical and chemical characteristics of ONOs from the Shevchenkovskoye cobalt–nickel ore deposit and other Kazakhstan deposits, highlighting the challenges they pose for conventional beneficiation and metallurgical processing. Current industrial practices are analyzed, including pyrometallurgical, hydrometallurgical, and pyro-hydrometallurgical methods, with an emphasis on their efficiency, environmental impact, and economic feasibility. Special attention is given to the potential of hydro-catalytic leaching as a flexible, energy-efficient alternative for treating low-grade ONOs under atmospheric conditions. The results underscore the necessity of developing cost-effective and sustainable technologies tailored to the unique composition of Kazakhstani ONOs, particularly those rich in iron and magnesium. This work provides a strategic framework for future research and the industrial application of advanced leaching techniques to unlock the full potential of Kazakhstan’s nickel resources. Full article
(This article belongs to the Section Extractive Metallurgy)
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24 pages, 2540 KiB  
Article
Classification Framework for Hydrological Resources for Sustainable Hydrogen Production with a Predictive Algorithm for Optimization
by Mónica Álvarez-Manso, Gabriel Búrdalo-Salcedo and María Fernández-Raga
Hydrogen 2025, 6(3), 54; https://doi.org/10.3390/hydrogen6030054 - 6 Aug 2025
Abstract
Given the urgent need to decarbonize the global energy system, green hydrogen has emerged as a key alternative in the transition to renewables. However, its production via electrolysis demands high water quality and raises environmental concerns, particularly regarding reject water discharge. This study [...] Read more.
Given the urgent need to decarbonize the global energy system, green hydrogen has emerged as a key alternative in the transition to renewables. However, its production via electrolysis demands high water quality and raises environmental concerns, particularly regarding reject water discharge. This study employs an experimental and analytical approach to define optimal water characteristics for electrolysis, focusing on conductivity as a key parameter. A pilot water treatment plant with reverse osmosis and electrodeionization (EDI) was designed to simulate industrial-scale pretreatment. Twenty water samples from diverse natural sources (surface and groundwater) were tested, selected for geographical and geological variability. A predictive algorithm was developed and validated to estimate useful versus reject water based on input quality. Three conductivity-based categories were defined: optimal (0–410 µS/cm), moderate (411–900 µS/cm), and restricted (>900 µS/cm). Results show that water quality significantly affects process efficiency, energy use, waste generation, and operating costs. This work offers a technical and regulatory framework for assessing potential sites for green hydrogen plants, recommending avoidance of high-conductivity sources. It also underscores the current regulatory gap regarding reject water treatment, stressing the need for clear environmental guidelines to ensure project sustainability. Full article
(This article belongs to the Special Issue Advances in Hydrogen Production, Storage, and Utilization)
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38 pages, 2180 KiB  
Review
Ternary Choline Chloride-Based Deep Eutectic Solvents: A Review
by Abdulalim Ibrahim, Marc Mulamba Tshibangu, Christophe Coquelet and Fabienne Espitalier
ChemEngineering 2025, 9(4), 84; https://doi.org/10.3390/chemengineering9040084 - 6 Aug 2025
Abstract
Ternary choline chloride-based deep eutectic solvents (TDESs) exhibit unique physicochemical properties, including lower viscosities, lower melting points, higher thermal stabilities, and enhanced solvations compared to binary deep eutectic solvents (BDESs). Although BDESs have been widely studied, the addition of a third component in [...] Read more.
Ternary choline chloride-based deep eutectic solvents (TDESs) exhibit unique physicochemical properties, including lower viscosities, lower melting points, higher thermal stabilities, and enhanced solvations compared to binary deep eutectic solvents (BDESs). Although BDESs have been widely studied, the addition of a third component in TDESs offers opportunities to further optimize their performance. This review aims to evaluate the physicochemical properties of TDESs and highlight their potential applications in sustainable industrial processes compared to BDESs. A comprehensive analysis of the existing literature was conducted, focusing on TDES properties, such as phase behavior, density, viscosity, pH, conductivity, and the effect of water, along with their applications in various fields. TDESs demonstrated superior physicochemical characteristics compared to BDESs, including improved solvation and thermal stability. Their applications in biomass conversion, CO2 capture, heavy oil upgrading, refrigeration gases, and as solvents/catalysts in organic reactions show significant promise for enhancing process efficiency and sustainability. Despite their advantages, TDESs face challenges including limited predictive models, potential instability under certain conditions, and scalability hurdles. Overall, TDESs offer significant potential for advancing sustainable and efficient chemical processes for industrial applications. Full article
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24 pages, 1777 KiB  
Article
Development of a Bacterial Lysate from Antibiotic-Resistant Pathogens Causing Hospital Infections
by Sandugash Anuarbekova, Azamat Sadykov, Dilnaz Amangeldinova, Marzhan Kanafina, Darya Sharova, Gulzhan Alzhanova, Rimma Nurgaliyeva, Ardak Jumagaziyeva, Indira Tynybayeva, Aikumys Zhumakaeva, Aralbek Rsaliyev, Yergali Abduraimov and Yerkanat N. Kanafin
Microorganisms 2025, 13(8), 1831; https://doi.org/10.3390/microorganisms13081831 - 6 Aug 2025
Abstract
Biotechnological research increasingly focuses on developing new drugs to counter the rise of antibiotic-resistant strains in hospitals. This study aimed to create bacterial lysates from antibiotic-resistant pathogens isolated from patients and medical instruments across hospital departments. Identification was performed based on morphological, cultural, [...] Read more.
Biotechnological research increasingly focuses on developing new drugs to counter the rise of antibiotic-resistant strains in hospitals. This study aimed to create bacterial lysates from antibiotic-resistant pathogens isolated from patients and medical instruments across hospital departments. Identification was performed based on morphological, cultural, and biochemical characteristics, as well as 16S rRNA gene sequencing using the BLAST algorithm. Strain viability was assessed using the Miles and Misra method, while sensitivity to eight antibacterial drug groups and biosafety between cultures were evaluated using agar diffusion. From 15 clinical sources, 25 pure isolates were obtained, and their phenotypic and genotypic properties were studied. Carbohydrate fermentation testing confirmed that the isolates belonged to the genera Escherichia, Citrobacter, Klebsiella, Acinetobacter, Pseudomonas, Staphylococcus, Haemophilus, and Streptococcus. The cultures exhibited good viability (109–1010 CFU/mL) and compatibility with each other. Based on prevalence and clinical significance, three predominant hospital pathogens (Klebsiella pneumoniae 12 BL, Pseudomonas aeruginosa 3 BL, and Acinetobacter baumannii 24 BL) were selected to develop a bacterial lysate consortium. Lysates were prepared with physical disruption using a French press homogenizer. The resulting product holds industrial value and may stimulate the immune system to combat respiratory pathogens prevalent in Kazakhstan’s healthcare settings. Full article
(This article belongs to the Special Issue Antimicrobial Resistance: Challenges and Innovative Solutions)
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19 pages, 3100 KiB  
Review
Casein-Based Biomaterials: Fabrication and Wound Healing Applications
by Nikolay Estiven Gomez Mesa, Krasimir Vasilev and Youhong Tang
Molecules 2025, 30(15), 3278; https://doi.org/10.3390/molecules30153278 - 5 Aug 2025
Abstract
Casein, the main phosphoprotein in milk, has a multifaceted molecular structure and unique physicochemical properties that make it a viable candidate for biomedical use, particularly in wound healing. This review presents a concise analysis of casein’s structural composition that comprises its hydrophobic and [...] Read more.
Casein, the main phosphoprotein in milk, has a multifaceted molecular structure and unique physicochemical properties that make it a viable candidate for biomedical use, particularly in wound healing. This review presents a concise analysis of casein’s structural composition that comprises its hydrophobic and hydrophilic nature, calcium phosphate nanocluster structure, and its response to different pH, temperature, and ionic conditions. These characteristics have direct implications for its colloidal stability, including features such as gelation, swelling capacity, and usability as a biomaterial in tissue engineering. This review also discusses industrial derivatives and recent advances in casein biomaterials based on different fabrication types such as hydrogels, electrospun fibres, films, and advanced systems. Furthermore, casein dressings’ functional and biological attributes have shown remarkable exudate absorption, retention of moisture, biocompatibility, and antimicrobial and anti-inflammatory activity in both in vivo and in vitro studies. The gathered evidence highlights casein’s versatile bioactivity and dynamic molecular properties, positioning it as a promising platform to address advanced wound dressing challenges. Full article
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