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16 pages, 288 KB  
Article
Impact of Natural Heat Stress on Pregnant Rabbits: Behavioral, Physiological, and Reproductive Changes and the Ameliorative Role of Curcumin and Vitamin D3
by Mahmoud Roshdy, Hassan A. Khalil, Doaa E. Saad, Mahmoud Kamal, Mostafa A. Ayoub, Yasser Alrauji and Mohamed Shehab-El-Deen
Vet. Sci. 2026, 13(5), 412; https://doi.org/10.3390/vetsci13050412 (registering DOI) - 22 Apr 2026
Abstract
This study evaluated the effects of heat stress on productive performance, physiology, reproduction, and oxidative status in pregnant New Zealand White (NZW) rabbit does, as well as the potential synergistic effects of curcumin and vitamin D3 (Cur + VD3) supplementation in [...] Read more.
This study evaluated the effects of heat stress on productive performance, physiology, reproduction, and oxidative status in pregnant New Zealand White (NZW) rabbit does, as well as the potential synergistic effects of curcumin and vitamin D3 (Cur + VD3) supplementation in alleviating these stress-induced impairments. Eighty multiparous does (12–18 months old) were assigned to a 2 × 2 factorial design involving two ambient temperatures (indoor vs. outdoor) and two supplementation levels (with or without Cur + VD3). Outdoor does experienced severe heat stress (THI = 33.22) compared to indoor thermal comfort conditions (THI = 25.13). The supplement (Cur + VD3) was administered orally at 1 mL/kg body weight. Heat stress significantly decreased body weight, milk yield, litter size, weight at weaning, and behavioral activity. Conversely, rectal temperature, respiration rate, and mortality increased. Supplementation with Cur + VD3 showed improved body weight, reproductive parameters, milk yield, and behavior, while reducing mortality (0% vs. 5%) compared to treatment without these additives. Physiologically, Cur + VD3 lowered rectal temperature and respiration rate. In conclusion, combined curcumin and vitamin D3 supplementation is an effective nutritional strategy to improve heat stress tolerance and maintain productivity in pregnant rabbits exposed to high ambient temperatures. Full article
29 pages, 2075 KB  
Article
Design and Deployment of an IoT-Based Digital Agriculture System in a Hydroponic Plant Factory
by Herrera-Arroyo Raul Omar, Moreno-Aguilera Cristal Yoselin, Coral Martinez-Nolasco, Víctor Sámano-Ortega, Mauro Santoyo-Mora and Martínez-Nolasco Juan José
Technologies 2026, 14(5), 247; https://doi.org/10.3390/technologies14050247 - 22 Apr 2026
Abstract
The incorporation of the Internet of Things (IoT) in indoor agricultural systems has become an essential tool for monitoring and analyzing environmental variables, contributing to more efficient decision-making. This article presents the design and implementation of an IoT-based digital agriculture system applied to [...] Read more.
The incorporation of the Internet of Things (IoT) in indoor agricultural systems has become an essential tool for monitoring and analyzing environmental variables, contributing to more efficient decision-making. This article presents the design and implementation of an IoT-based digital agriculture system applied to a Plant Factory (PF) for hydroponic vegetable cultivation using the Nutrient Film Technique (NFT). The objective of this study was to develop a system capable of effectively monitoring and controlling the environmental variables that directly influence the microclimate of a closed agricultural environment. The proposed system integrates a four-layer IoT architecture based on a MODBUS RS-485 communication bus, which allows for continuous data acquisition and the operation of multiple sensors and controlled devices. Additionally, user-oriented tools such as a human–machine interface (HMI), a web application, a mobile application and an automatic alert module were incorporated, enhancing accessibility and remote supervision. Experimental results showed stable control performance of ambient temperature (TA), relative humidity (RH), photoperiod, and photosynthetic photon flux density (PPFD), along with continuous monitoring of CO2 concentration. A 30-day validation experiment using Swiss chard (Beta vulgaris L. var. cicla) under controlled conditions was conducted. The results showed progressive plant development, with leaf area increasing from 15.17 cm2 to 690.39 cm2, plant height from 7 cm to 31 cm, fresh weight from 23 g to 171 g, and the number of leaves from 9 to 20. These results support the functional validity of the proposed system as a reliable platform for environmental monitoring and control in controlled-environment agriculture. Full article
(This article belongs to the Special Issue IoT-Enabling Technologies and Applications—2nd Edition)
32 pages, 1803 KB  
Article
Restorative Effects of Screen-Based Interactive Digital Multimedia in Urban Interiors: The Role of Feedback Intensity and Color Hue
by Shimeng Hao, Huanying Sun, Yisong Zhang and Hua Zhong
Sustainability 2026, 18(9), 4174; https://doi.org/10.3390/su18094174 - 22 Apr 2026
Abstract
Urban residents require space-efficient interventions to mitigate chronic stress. While indoor digital nature shows promise, the precise impact of interactive design parameters remains unclear. This study investigated how interactive feedback intensity (none, slow, fast) and color hue (neutral, warm, cool) influence psychological and [...] Read more.
Urban residents require space-efficient interventions to mitigate chronic stress. While indoor digital nature shows promise, the precise impact of interactive design parameters remains unclear. This study investigated how interactive feedback intensity (none, slow, fast) and color hue (neutral, warm, cool) influence psychological and physiological restoration. Following negative emotion induction, healthy participants engaged in within-subject conditions evaluated via multimodal assessments, including EEG, HRV, and subjective scales (PANAS, PRS, SAM/PAD). Results identified interactive feedback intensity as the primary driver of restoration. Specifically, fast feedback improved positive affect by up to 20.4% and reduced negative affect by 20.8% compared to passive self-restoration. Neurologically, interactive engagement was associated with elevated EEG alpha-band activity by up to 97.8% relative to standing controls, a pattern consistent with cortical relaxation. Furthermore, while physical interaction was uniformly associated with physiological indices broadly consistent with recovery, color hue significantly moderated subjective outcomes. Neutral and warm hues generated significantly higher overall perceived restorativeness (M = 73.18 and M = 70.14, respectively) than the self-restoration control (M = 61.26). Notably, neutral tones were uniquely associated with modest changes in HRV time-domain indices suggestive of parasympathetic autonomic modulation. These findings provide actionable, empirically validated guidelines for deploying responsive digital interventions to support mental well-being in dense urban interiors. Full article
42 pages, 2032 KB  
Perspective
The Therapeutic Home Environment for Chronic Diseases: A Transdisciplinary Ecosystem for Achieving Migraine Freedom and Managing Comorbid Anxiety, Insomnia, and Chronic Pain
by Dorothy Day Huntsman, Desiree Jenkinson and Grzegorz Bulaj
Healthcare 2026, 14(9), 1123; https://doi.org/10.3390/healthcare14091123 - 22 Apr 2026
Abstract
Home has been recognized as a health infrastructure through hospital-at-home, home care, and direct-to-consumer wellness and fitness products. However, the patient home environment has been largely overlooked by healthcare as a means to improve therapy outcomes for difficult-to-treat chronic conditions, such as migraine; [...] Read more.
Home has been recognized as a health infrastructure through hospital-at-home, home care, and direct-to-consumer wellness and fitness products. However, the patient home environment has been largely overlooked by healthcare as a means to improve therapy outcomes for difficult-to-treat chronic conditions, such as migraine; high-impact pain; and treatment-resistant depression, anxiety, or insomnia. Growing research evidence enables the formulation of a therapeutic home environment standard consisting of three pillars: biophilic design, indoor environmental quality, and intentional self-care spaces that serve as habit cues and foster sleep hygiene, stress management, relaxation, physical activity, and social interactions. Together, these environmental and behavioral interventions can transform real-world inputs into clinical benefits through autonomic, circadian, and emotional regulation. We also highlight the converging roles of self-management, self-efficacy, self-regulation, and self-compassion in sustaining patient engagement and healing at home. The applicability of the therapeutic home environment as an adjunct is illustrated in the case of chronic migraine, a debilitating neurological condition commonly associated with comorbidities. Current challenges in achieving migraine freedom with FDA-approved pharmacotherapies, neuromodulation devices, and digital health technologies are underscored by the high prevalence of refractory, chronic, episodic, and pediatric migraine. Perspectives on developing a personalized, multimodal cure for migraine are illustrated through a hypothetical drug + digital combination therapy comprising anti-CGRP drugs and an AI-powered digital health platform that promotes daily self-care practices within the therapeutic home environments. In conclusion, achieving sustained freedom from high-morbidity conditions requires end-to-end care ecosystems that integrate pharmacological, cognitive, behavioral, and environmental interventions into real-world settings. Full article
(This article belongs to the Special Issue Multidisciplinary Approaches to Chronic Disease Management)
25 pages, 5996 KB  
Article
Experimental and Numerical Simulation Studies on the Interface Characteristics Model of Loess and Bamboo Geogrid
by Xiaodong Liang, Guozhou Chen, Mingming Cao and Zibo Du
Appl. Sci. 2026, 16(8), 4055; https://doi.org/10.3390/app16084055 - 21 Apr 2026
Abstract
The widespread loess in western China poses significant challenges to transportation infrastructure construction due to its water sensitivity and collapsibility. This study investigates the interface mechanical properties of bamboo geogrid-reinforced loess under static loading through large-scale indoor pull-out tests and DEM–FDM coupled numerical [...] Read more.
The widespread loess in western China poses significant challenges to transportation infrastructure construction due to its water sensitivity and collapsibility. This study investigates the interface mechanical properties of bamboo geogrid-reinforced loess under static loading through large-scale indoor pull-out tests and DEM–FDM coupled numerical simulations. The effects of vertical stress, the pull-out rate, the number of transverse ribs, burial depth, and reinforcement material on interface behavior were systematically evaluated. Results show that peak pull-out force increases with vertical stress, the number of transverse ribs, and burial depth, with all curves exhibiting pronounced strain hardening followed by softening characteristics. The pull-out rate exhibits a non-monotonic effect, with peak resistance higher at both lower and higher rates compared to intermediate rates. Bamboo geogrids demonstrate substantially superior performance over geogrids, with approximately four times higher peak pull-out resistance and greater initial stiffness. Numerical analysis reveals increased porosity and decreased coordination number in the grid vicinity, the horizontal stratification of the slip rate along the reinforcement, and concentration of strong force chains ahead of transverse ribs, elucidating the model-derived mechanisms underlying the macroscopic reinforcement effects. The findings confirm that bamboo geogrids provide effective and sustainable reinforcement for loess subgrades, offering a scientific basis for environmentally friendly engineering applications in loess regions. Although potential long-term durability under field environmental conditions requires further verification, the superior mechanical interface performance demonstrated here positions treated bamboo geogrids as a promising sustainable reinforcement option. Full article
(This article belongs to the Section Civil Engineering)
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26 pages, 13965 KB  
Article
Experimental Characterization of a 3D-Printed Conformal Array Antenna for 2.4 GHz WiFi Backscatter
by Muhammed Yusuf Onay and Burak Dokmetas
Electronics 2026, 15(8), 1758; https://doi.org/10.3390/electronics15081758 - 21 Apr 2026
Abstract
This article presents the experimental characterization of a 3D-printed conformal 2×1 microstrip array antenna designed for 2.4 GHz WiFi backscatter applications in indoor IoT scenarios. Starting from a planar configuration, three conformal states (30, 60, and [...] Read more.
This article presents the experimental characterization of a 3D-printed conformal 2×1 microstrip array antenna designed for 2.4 GHz WiFi backscatter applications in indoor IoT scenarios. Starting from a planar configuration, three conformal states (30, 60, and 90) were realized to systematically evaluate the effect of bending. Detailed simulation and measurement results were obtained in terms of gain, efficiency, and radiation patterns, with the measured gain decreasing from 9.4 dBi in the flat case to 6.2 dBi at 90 bending. To evaluate the system-level impact of these measured gain variations, the measured power levels were incorporated into a TDMA-based WiFi backscatter link model, and the achievable bit transmission rate was assessed under practical indoor conditions, including line-of-sight (LoS), non-line-of-sight (NLoS), and residual interference effects. The main contribution of the work lies in combining the experimental validation of a fully 3D-printed RF-grade conformal antenna with a system-level WiFi backscatter assessment. The combined analytical–experimental results indicate that increasing curvature reduces the achievable maximum bit transmission rate and leads to earlier infeasibility under tighter quality of service (QoS) thresholds within the tested 2.4 GHz indoor WiFi backscatter conditions, suggesting that conformal geometry is an important design consideration for the studied setup. Full article
(This article belongs to the Section Microwave and Wireless Communications)
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19 pages, 7383 KB  
Article
Water Retention and Evaporation Dynamics of Mineral Growing Media for Indoor Horticulture Systems
by Jolan Schabauer, Erich Streit, Azra Korjenic, Jitka Peterková, Jiří Zach and Abdulah Sulejmanovski
Horticulturae 2026, 12(4), 501; https://doi.org/10.3390/horticulturae12040501 - 21 Apr 2026
Abstract
Mineral substrates for indoor horticulture systems critically determine plant water availability and irrigation demand. However, integrative assessments linking pore structure, water retention, and evaporation dynamics of commonly used mineral growing media remain scarce. A total of nine distinct mineral substrates were investigated: expanded [...] Read more.
Mineral substrates for indoor horticulture systems critically determine plant water availability and irrigation demand. However, integrative assessments linking pore structure, water retention, and evaporation dynamics of commonly used mineral growing media remain scarce. A total of nine distinct mineral substrates were investigated: expanded clay, expanded slate, pumice, perlite, zeolite, vermiculite, lava granules, brick chips, and clay granules. To assess the impact of granulometry, pumice was tested in three different grain sizes (1–3 mm, 4–7 mm, 7–14 mm), resulting in a total of 11 experimental samples. Samples were characterized using scanning electron microscopy (SEM), suction experiments, and evaporation tests at 30%, 50%, and 70% relative humidity (RH) at 23 °C. Bulk density ranged from <0.12 g·cm−3 (perlite, vermiculite) to >0.99 g·cm−3 (zeolite, brick chips), while volumetric water content varied from 11.0 vol.% (expanded clay) to 46.6 vol.% (vermiculite). Plant-available water content (AWC) ranged from 2.7 vol.% (expanded clay) to 30.9 vol.% (clay granules). These results demonstrate that pore interconnectivity, rather than total porosity, is the decisive driver of hydraulic performance. Finer pumice fractions increased water retention by ~16% compared to coarser fractions. All substrates exhibited a two-phase evaporation profile, with initial rates ranging from 1.9 to 5.6 g·h−1 at 30% RH. Clay granules showed the most temporally stable evaporation, with only a 37% rate reduction over 48 h, compared to 66% for perlite. While conducted under controlled laboratory conditions, these findings provide a quantitative basis for targeted substrate selection and blending to optimize root-zone hydration, irrigation efficiency, and hygrothermal performance in permanent indoor horticulture systems. Full article
(This article belongs to the Section Floriculture, Nursery and Landscape, and Turf)
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16 pages, 2151 KB  
Article
Energy Profiling of Solar-Powered Smart Hydroponic Systems in Kazakhstan
by Ali Serikov, Yerassyl Olzhabay, Alikhan Talipbayev, Damir Aidarkhanov and Annie Ng
Energies 2026, 19(8), 1994; https://doi.org/10.3390/en19081994 - 21 Apr 2026
Abstract
Kazakhstan, the largest landlocked country and the ninth-largest country by land area in the world, played a central role in the Soviet Virgin Lands campaign. However, decades of cultivation left the soil degraded and vulnerable to erosion. This legacy, along with worldwide water [...] Read more.
Kazakhstan, the largest landlocked country and the ninth-largest country by land area in the world, played a central role in the Soviet Virgin Lands campaign. However, decades of cultivation left the soil degraded and vulnerable to erosion. This legacy, along with worldwide water scarcity, drives the search for alternative farming methods such as hydroponics. This study investigates the feasibility of powering an indoor hydroponic system with photovoltaic (PV) technology in different regions of Kazakhstan. Three PV configurations, 16, 20, and 24 panels, were simulated in PVsyst (8.0.12) to meet the monthly energy demand of the system. The goal was to determine the minimum PV size and storage capacity for continuous year-round operation. Results showed that 16 panels were sufficient only from April to July, whereas 20- and 24-panel systems provided better reliability throughout the year. Optimal designs varied by region. For instance, those in the south, such as Turkistan, required smaller setups (6.8 kWp, 26 panels, 7 batteries), whereas those in the north, such as Akmola, needed larger ones (10.9 kWp, 42 panels, 10 batteries). Performance ratios ranged from 41% to 66% depending on the region. These results indicate that PV-powered hydroponic systems are feasible in Kazakhstan, although system configurations must be adapted to specific regional solar conditions. Full article
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20 pages, 3603 KB  
Article
Demand-Driven Ozone-Assisted Oxidation in a Recirculating Domestic Kitchen Hood: Experimental Evaluation and RSM Optimization
by Erdener Özçetin, Cenk İçöz and Adil Hasan Ünal
Appl. Sci. 2026, 16(8), 4022; https://doi.org/10.3390/app16084022 - 21 Apr 2026
Abstract
Cooking-related emissions represent a major contributor to indoor air pollution in residential kitchens, producing complex mixtures of volatile organic compounds (VOCs), odor-causing gases, oil vapors, particulate matter (PM2.5), and combustion-related pollutants (CO and NOx). In this study, a controlled [...] Read more.
Cooking-related emissions represent a major contributor to indoor air pollution in residential kitchens, producing complex mixtures of volatile organic compounds (VOCs), odor-causing gases, oil vapors, particulate matter (PM2.5), and combustion-related pollutants (CO and NOx). In this study, a controlled ozone-assisted oxidation approach was integrated into a recirculating (ductless) domestic kitchen hood equipped with a confined reaction chamber and experimentally evaluated under closed-loop operating conditions where treated air was returned to the indoor environment after post-treatment. A multivariate Response Surface Methodology (RSM) framework based on the Box–Behnken design was employed to quantify and optimize the coupled effects of temperature (20–30 °C), relative humidity (40–60%), ozone dosage (1–3 ppm within the confined reaction zone), and airflow rate (150–250 m3/h) on multi-pollutant removal performance. The results demonstrate that ozone assistance substantially improves the abatement of oxidation-sensitive pollutants, particularly VOCs and odor, while airflow rate strongly governs transport-dominated pollutants such as PM2.5 and oil vapors. In contrast, CO and NOx exhibited limited improvement, indicating that ozone-assisted oxidation alone is insufficient for comprehensive control of combustion-related gases under short-residence-time recirculating hood conditions. The main contribution of this work is the implementation of a demand-driven ozone management strategy, supported by dual ozone sensing for reaction-zone control and outlet safety verification, where ozone generation is activated only in the presence of reactive gaseous pollutants and automatically reduced or terminated once pollutant concentrations fall below predefined thresholds, minimizing unnecessary oxidant release. Residual ozone downstream of the reaction stage was continuously monitored to prevent excess ozone return to the occupied zone. Overall, the proposed closed-loop, feedback-controlled ozone-assisted recirculating range hood concept demonstrated device-level reductions in measured VOC/odor signals under controlled conditions, while also highlighting the need for complementary post-treatment components for particle- and combustion-related pollutants. However, the potential formation of secondary oxidation byproducts was not characterized in this study, and therefore the results should be interpreted with respect to device-level pollutant removal rather than comprehensive indoor air quality improvement. Full article
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24 pages, 550 KB  
Review
ISO 16000-8 and Ventilation Performance: A Critical Review
by Sascha Nehr and Julia Hurraß
Standards 2026, 6(2), 16; https://doi.org/10.3390/standards6020016 - 20 Apr 2026
Abstract
Standard 16000-8 of the International Organization for Standardization (ISO 16000-8) specifies the assessment of ventilation performance using age-of-air concepts and tracer gas techniques. Since its publication in 2007, ventilation systems and assessment practices have evolved considerably, driven by increased use of mixed-mode and [...] Read more.
Standard 16000-8 of the International Organization for Standardization (ISO 16000-8) specifies the assessment of ventilation performance using age-of-air concepts and tracer gas techniques. Since its publication in 2007, ventilation systems and assessment practices have evolved considerably, driven by increased use of mixed-mode and decentralized ventilation and advances in modeling and measurement technologies. This review examines how ISO 16000-8 can be modernized to harmonize with adjacent ventilation and indoor air quality standards while remaining applicable to contemporary systems and emerging approaches. A structured literature search of Web of Science and Google Scholar identified 76 studies (2007–2026) that engage with ISO 16000-8, age-of-air metrics, or tracer gas-based assessment. The literature was synthesized qualitatively using the framework of Preferred Reporting Items for Systematic reviews and Meta-Analyses (PRISMA), classifying studies into performance assessment, measurement–simulation convergence, and standardization discourse. The synthesis shows that while the conceptual foundations of ISO 16000-8 remain valid, assumptions of homogeneous mixing and steady-state conditions are often violated in real buildings, leading to inconsistent application of age-of-air indicators. Field and laboratory studies under point-source conditions demonstrate reduced ventilation effectiveness of 0.73–0.82 in classrooms and 0.5–1.4 in various indoor environments, instead of ≈1 for perfect mixing. Spatial heterogeneity is also observed in mixed-mode systems, with an efficiency around 0.5. In decentralized and façade-integrated systems, air exchange effectiveness deviates from theoretical expectations, indicating inhomogeneous air renewal and short-circuiting. Field measurements show configuration-dependent discrepancies in air exchange rates (e.g., carbon dioxide vs. perfluorocarbon tracer methods under varying door positions), while wind induces time-varying infiltration. Collectively, the literature demonstrates systematic violations of well-mixed and steady-state assumptions underpinning ISO 16000-8. Fragmentation between ventilation performance standards and indoor air quality regulation limits practical uptake. Emerging experimental, numerical, and data-driven methods complement ISO 16000-8, provided applicability domains and uncertainties are addressed. The review concludes that ISO 16000-8 should be modernized toward a harmonized, performance-based framework integrating diverse ventilation systems and assessment technologies. Full article
(This article belongs to the Section Building Standards)
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21 pages, 1094 KB  
Article
Empirical Measurement of Eucalyptus nitens Water Vapour Diffusion Resistivity at 23 °C and 50% RH
by Zahraa Al-Shammaa, Mark Dewsbury, Louise Wallis and Hartwig Künzel
Forests 2026, 17(4), 511; https://doi.org/10.3390/f17040511 - 20 Apr 2026
Abstract
Quantifying moisture transport through building envelope materials is vital for durability, energy efficiency, and healthy indoor environments. Water vapour diffusion resistivity (µ-value) is a key parameter for hygrothermal modelling, moisture control, and mould risk assessment. Globally, data for solid wood species are scarce, [...] Read more.
Quantifying moisture transport through building envelope materials is vital for durability, energy efficiency, and healthy indoor environments. Water vapour diffusion resistivity (µ-value) is a key parameter for hygrothermal modelling, moisture control, and mould risk assessment. Globally, data for solid wood species are scarce, and in Australia—despite the rising use of plantation-grown timber—critical hygrothermal properties remain undocumented. To close this gap, this study experimentally evaluated Eucalyptus nitens, a plantation-grown hardwood widely used in Australian construction. Solid-wood specimens prepared from industry-sourced boards were tested at 23 °C and 50% RH using both the wet-cup and dry-cup methods of the gravimetric technique. For wet-cup tests, µ-values ranged from 24 to 33; for dry-cup tests, µ-values ranged from 179 to 273, showing clear variability linked to differences in relative humidity. Experimental issues included surface cupping, sealing integrity, and extended equilibration time during dry-cup testing. These findings provide the first empirical µ-value dataset for E. Nitens under moderate-humidity conditions, delivering essential input parameters for hygrothermal models and supporting moisture-safe, energy-efficient design strategies for the broader construction sector. Full article
14 pages, 1708 KB  
Article
Establishment of Laboratory Bioassay System for Phyllotreta striolata Larvae and Screening of Novel Bt Cry Proteins
by Leqi Wang, Zhenyi Liu, Ivan M. Dubovskiy, Changlong Shu, Jie Zhang, Junjie Zhang, Wenmei Du and Qi Peng
Toxins 2026, 18(4), 191; https://doi.org/10.3390/toxins18040191 - 20 Apr 2026
Abstract
Phyllotreta striolata is a global pest of cruciferous vegetables, and controlling its soil-dwelling larvae is challenging. The lack of standardized larval bioassay methods hinders the screening of effective biocontrol agents. In this study, we established a stable and standardized laboratory-efficacy trial system for [...] Read more.
Phyllotreta striolata is a global pest of cruciferous vegetables, and controlling its soil-dwelling larvae is challenging. The lack of standardized larval bioassay methods hinders the screening of effective biocontrol agents. In this study, we established a stable and standardized laboratory-efficacy trial system for P. striolata larvae. Indoor rearing techniques were optimized for Brassica juncea var. foliosa and Brassica juncea var. megarrhiza were identified as the optimal host plants, with ideal oviposition conditions at 26–28 °C using black flannel substrate, and soil-cultured Brassica rapa var. pekinensis as the host plant. Based on these findings, a larval bioactivity assay was established using B. juncea var. megarrhiza slices on water-agar. This system maintained a natural larval mortality rate below 5% within 48 h, meeting the bioassay requirements. The reliability of the system was validated by evaluating the activity of the engineered Bacillus thuringiensis (Bt) strain G033A against larvae, where the LC50 value decreased from 23.013 mg/mL to 7.295 mg/mL with an extended treatment time (12–48 h). Using this standardized method, novel Cry proteins with high activity against P. striolata larvae were screened. Cry8Ca and Cry8Ga proteins exhibited LC50 values of 2.243 mg/mL and 1.649 mg/mL, respectively. Full article
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28 pages, 8935 KB  
Article
Wind-Sound Synergy and Fractal Design: Intelligent, Adaptive Acoustic Façades for High-Performance, Climate-Responsive Buildings
by Lingge Tan, Xinyue Zhang, Donghui Cui and Stephen Jia Wang
Buildings 2026, 16(8), 1615; https://doi.org/10.3390/buildings16081615 - 20 Apr 2026
Abstract
The building façade serves as the primary interface between the built environment and external climate, marking the transition from static regulation to dynamic response in climate-adaptive design. While existing research predominantly addresses periodic climatic elements such as temperature and solar radiation, the highly [...] Read more.
The building façade serves as the primary interface between the built environment and external climate, marking the transition from static regulation to dynamic response in climate-adaptive design. While existing research predominantly addresses periodic climatic elements such as temperature and solar radiation, the highly stochastic wind environment and its potential for internal acoustic problems remain systematically unexplored. This study investigates the acoustic modulation mechanism of building façades under dynamic wind conditions through a simulation-based methodology. The primary aim is to demonstrate the use of active control to mitigate the influence of fluctuating wind on the internal acoustic environment of buildings with open windows or semi-open boundaries, focusing on the coupling between stochastic wind fields and architectural acoustics in humid subtropical climates. We propose a wind-responsive adaptive acoustic façade system employing fractal geometry and configurable delay strategies, and develop a high-fidelity simulation framework to quantify how façade geometry and activation logic regulate acoustic parameters under varying wind conditions (1–8 m/s). Results indicate that: (1) support vector regression-based mapping of wind speed to delay strategies maintains key sound-field parameters (Lateral Fraction (LF), Speech Clarity (C50), and Early Decay Time to Reverberation Time ratio (EDT/RT30)) within 10% fluctuation across wind regimes; (2) fractal configurations achieve balanced wide-band (125 Hz–8 kHz) performance, with SPL fluctuation <3 dB, spectral tilt (+0.3 dB), and reverberation time slope <0.3; (3) configurational switching between column (high LF) and row (high C50) arrangements enables dynamic trade-off between spatial impression and speech clarity. This work establishes an integrated framework coupling wind dynamics, façade morphology, and acoustic modulation to regulate objective indoor acoustic parameters. Based on the simulated omnidirectional point-source model, the results show that key acoustic indicators remain stable across varying wind conditions, providing a theoretical and quantifiable basis for climate-responsive acoustic envelope design. Future work will include empirical prototype testing and listening tests to determine whether these simulated acoustic parameters translate into improved comfort and well-being for occupants. Full article
(This article belongs to the Special Issue Advanced Research on Improvement of the Indoor Acoustic Environment)
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15 pages, 1320 KB  
Article
An Exploratory Study of Airborne Fungal Contamination and Its Association with Microclimate Conditions as Regards Sustainable Zoo Development
by Mario Ostović, Ivica Pučko, Anamaria Ekert Kabalin, Danijela Horvatek Tomić, Sven Menčik, Željko Pavičić, Nevenka Rudan, Ingeborg Bata, Dijana Beneta and Kristina Matković
Sustainability 2026, 18(8), 4007; https://doi.org/10.3390/su18084007 - 17 Apr 2026
Viewed by 209
Abstract
Air quality management in zoological gardens plays a crucial role in their sustainable development. However, air quality in these settings remains understudied. In addition, previous research has largely focused on airborne microbial contamination merely in animal enclosures. This exploratory study provides preliminary insights [...] Read more.
Air quality management in zoological gardens plays a crucial role in their sustainable development. However, air quality in these settings remains understudied. In addition, previous research has largely focused on airborne microbial contamination merely in animal enclosures. This exploratory study provides preliminary insights into airborne fungal contamination alongside microclimate conditions in the visitor and worker areas of animal premises in the Zagreb Zoo. The study was performed in the Monkey House, Tropical House, Rainy Africa, and Bird House, as well as outdoors in fall. Fungi were identified based on macroscopic and microscopic examinations. Total culturable fungal concentration in indoor air ranged between 50 and 4.25 × 103 CFU/m3, and in outdoor air between 1.00 × 102 and 1.50 × 103 CFU/m3. Molds of eight genera and yeasts were isolated from the air. Both indoors and outdoors, the predominant genera were Cladosporium and Penicillium, and also genus Aspergillus indoors. Cladosporium spp. and Penicillium spp. concentrations, as well as total fungal concentration in the air, were on average, highest in Rainy Africa and Bird House, while the highest average Aspergillus spp. concentration was found in the Tropical House. Levels of Cladosporium spp., Penicillium spp., and Aspergillus spp. concentrations were associated with microclimate conditions. Study results suggest that the airborne fungal contamination may depend on the animals housed in the premises, and the design and management of the premises. Although total fungal concentration determined may not necessarily pose a health risk for exposed people, the qualitative composition of fungi signifies the importance of implementing good practices in zoo premises, including optimal microclimate conditions and effective ventilation. The results obtained also indicate the need for air quality monitoring, which concurs with zoo sustainability goals. Full article
(This article belongs to the Section Pollution Prevention, Mitigation and Sustainability)
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23 pages, 4828 KB  
Article
A Compact and Robust Framework for Multi-Condition Transient Pressure-Wave-Based Leakage Identification in District Heating Networks
by Chang Chang, Xiangli Li, Xin Jia and Lin Duanmu
Buildings 2026, 16(8), 1586; https://doi.org/10.3390/buildings16081586 - 17 Apr 2026
Viewed by 208
Abstract
Leakage identification in district heating networks is challenging because leakage-induced transient pressure waves often overlap with pressure disturbances triggered by routine operations such as valve regulation, pump speed variation, and emergency shut-off. In addition, the scarcity of high-quality labeled leakage samples limits the [...] Read more.
Leakage identification in district heating networks is challenging because leakage-induced transient pressure waves often overlap with pressure disturbances triggered by routine operations such as valve regulation, pump speed variation, and emergency shut-off. In addition, the scarcity of high-quality labeled leakage samples limits the robustness of data-driven models under small-sample conditions. To address these issues, this study proposes a compact and moderately interpretable framework for multi-condition identification from transient pressure-wave signals, integrating signal preprocessing, handcrafted statistical feature extraction, multiclass ReliefF-based feature selection, and class-wise generative adversarial network augmentation in the selected feature space. A dataset containing four representative conditions, namely leakage, valve regulation, pump speed regulation, and emergency valve shut-off, was constructed using an integrated indoor district heating network testbed. After Hampel-based spike suppression and zero-phase Butterworth band-pass filtering within 0.5 to 300 Hz, time- and frequency-domain statistical features were extracted, and a compact subset was selected by multiclass ReliefF. A class-wise generative adversarial network was then used to augment the training set in feature space, while all evaluations were performed strictly on real samples. The results show that feature-space augmentation improves robustness and generalization under operational disturbances and noise. Using random forest as the representative classifier, Accuracy and Macro-F1 increased from 0.960 to 0.985, while leakage recall improved from 0.920 to 0.980. Further comparisons confirmed that the ReliefF-selected subset outperformed representative alternatives such as LASSO and mRMR. Overall, the proposed framework provides an effective solution for distinguishing leakage events from operational disturbances and offers practical support for online monitoring and intelligent operation of district heating networks. Full article
(This article belongs to the Special Issue Building Physics: Towards Low-Carbon and Human Comfort)
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