Search Results (3,568)

Oxidative stress and inflammation play a key role in many diseases. This study evaluated the potential of bioactive compounds from Red-belted Bracket and Artist’s Bracket mushrooms to mitigate these processes. Multistep extraction yielded fractions with diversified composition (triterpenoids, polysaccharides) and bioactivities, including antioxidant properties and inhibition of pro-inflammatory enzymes. Both species were rich in triterpenoids: ethanolic extracts from Artist’s Bracket contained mainly ganoderenic and ganoderic acids (≈31 μg/g d.w.), while Red-belted Bracket extracts contained phenolic acids (≈20 μg/g d.w., mainly vanillic and chebulic acids) and triterpenoids (≈73 μg/g d.w., mainly forpinic and formipinic acids). The alkaline and ethanolic extracts exhibited the highest radical scavenging and reducing activities. Lipoxygenase was inhibited only by ethanolic extracts, with IC₅₀ values of 0.93 mg d.w./mL for Artist’s Bracket (mixed inhibition) and 0.62 mg d.w./mL for Red-belted Bracket (noncompetitive). Artist’s Bracket was also a potent source of xanthine oxidase inhibitors acting uncompetitively (IC₅₀ = 0.71, 1.39, and 2.06 mg d.w./mL for ethanolic, methanolic, and aqueous extracts, respectively). In contrast, Red-belted Bracket was less active (IC₅₀ = 3.84 mg d.w./mL, noncompetitive). In conclusion, these mushrooms, particularly their ethanolic extracts, are promising sources of compounds with antioxidant and anti-inflammatory activities, acting as effective inhibitors of lipoxygenase and xanthine oxidase. Full article

Freezing effectively extends the shelf life of food and maintains product quality by inhibiting microorganisms, enzyme activity, and chemical reactions. However, issues such as ice crystal formation, protein denaturation, lipid oxidation, and the low-temperature adaptability of psychrophilic microorganisms during the freezing process can directly affect the final quality of frozen foods. Among these, the size and distribution of ice crystals are key factors determining the extent of tissue damage. Therefore, this review aims to identify innovative and optimized freezing and frozen storage strategies. In order to save energy and improve product quality, various new technologies have emerged in recent years, such as ultrasonic-assisted freezing, high-pressure freezing, and magnetic-field-assisted freezing. This study systematically discusses the principles, applications, and impact mechanisms of these technologies on frozen foods. Furthermore, this study proposes the future development trends of frozen foods, filling the gap in the current food industry where there is a lack of systematic discussion and evaluation of frozen foods. It provides technical support and research directions for continuous development and innovation in the field of frozen foods. Full article

The global methane budget is largely driven by biogenic sources, many of which remain insufficiently characterized. Here, we investigated the community composition and seasonal dynamics of methanogenic and methanotrophic assemblages to elucidate the key contributors to methane cycling and the environmental factors shaping these processes in lake sediments of the Rila Mountains (Bulgaria). Methanogenic communities are primarily composed of Methanothrix, Methanosarcina, Methanobacterium and Methanoregula with summer peaks in Methanothrix and Methanoregula, and cold-season proliferation of Methanobacterium. Methanotrophic communities are dominated by representatives of the Pseudomonadota, including Crenothrix, Methylobacter, and Methylocystis with summer maxima observed for Crenothrix and Methylobacter. Methanosarcina and Methylocystis showed relatively stable abundances throughout the ice-free season. Ordination and correlation analyses revealed that temperature, pH, and carbon (organic and inorganic) concentration and lability emerged as the environmental drivers influencing on microbial communities, with seasonally variable effects on methane-cycling microorganisms. These findings provide a foundation for future research on methane cycling in alpine lake ecosystems of the Rila Mountains and contribute to improving predictions of methane emissions under changing climatic conditions. Full article

To address the urgent need for electronics operable in extremely high-temperature environments, this paper presents a novel three-terminal, dual-gate, lateral 4H-SiC n-channel depletion-mode junction field effect transistor (JFET) without a backside electrode. Featuring a fully planar electrode layout, the device eliminates the back-gate effect and significantly improves integration compatibility. Experimental results demonstrate stable DC operation up to 500 °C, with an intrinsic gain of 9.79 at room temperature and 6.01 at 500 °C. Comparison with TCAD simulations confirms excellent agreement in the key physical trends of threshold voltage drift and mobility degradation, though quantitative discrepancies are observed and attributed to process-induced parasitic effects such as non-ideal ohmic contacts and interface states. Analysis shows that the new structure broadens the channel depletion layer by optimizing the depletion profile, thereby suppressing channel-length modulation and improving both output resistance and gate control. This work not only provides an effective device platform for high-temperature 4H-SiC analog integrated circuits (ICs) but also deepens the understanding of process-performance correlations, offering clear guidance for process-oriented device optimization. The proposed structure serves as a foundation for developing fully planar, high-temperature 4H-SiC analog ICs with promising potential in aerospace, automotive, and energy exploration systems. Full article

To address the need for dynamic and static parameter testing of IGBT modules, an integrated test platform was developed. In production-line applications, consistency differences in key parameters between the upper and lower bridge arms of manufactured modules were found to be difficult to quantify using a single metric. To overcome this limitation, a multi-parameter consistency evaluation method was proposed. Based on more than 400 sets of production-level measurements obtained from newly manufactured IGBT modules from the same batch, eight key parameters, including E_on, E_off, dv/dt, di/dt, V_CES, I_CES, V_GEth and I_GES, were selected to construct paired samples of the upper and lower bridge arms. A dimensionless consistency index (CI) was introduced to quantify relative deviations between bridge arms, and consistency characteristics were analyzed using raw-parameter boxplots, CI boxplots, and paired scatter plots in terms of distribution, discrepancy quantification, and sample-wise pairing. The results show that different parameters exhibit different levels of distributional difference and relative deviation between the two bridge arms under identical test conditions, and that the three analyses reveal mutually consistent trends. The proposed method provides an effective basis for within-batch quality assessment, bridge-arm matching, abnormal sample identification, and production process troubleshooting. Full article

Treatment-refractory rectal cancer liver metastasis represents a major therapeutic challenge, particularly in the absence of actionable genomic biomarkers. Functional precision oncology approaches integrating genomic profiling with patient-derived organoid (PDO) drug testing may provide biologically informed therapeutic prioritization. A 50-year-old female patient with KRAS/TP53-mutant, microsatellite-stable (MSS) rectal adenocarcinoma refractory to FOLFIRINOX was enrolled. A liver metastasis from a treatment-refractory rectal cancer patient was processed to establish three-dimensional patient-derived organoids. Histopathological concordance was assessed using H&E and p53 immunohistochemistry. Comprehensive genomic profiling was performed using a 637-gene targeted next-generation sequencing panel, enabling detection of single-nucleotide variants, indels, copy number variations, microsatellite instability, and tumor mutational burden. Functional drug sensitivity profiling was conducted in parallel 2D and 3D platforms using a customized 17-agent panel, followed by exploratory combinatorial validation. The organoids demonstrated high phenotypic and genomic concordance with the parental tumor, preserving key driver alterations (KRAS^A146T, TP53^R175H, APC frameshifts, CCNE1 amplification), microsatellite stability, and low tumor mutational burden (TMB: 6.37 mut/Mb). Functional screening identified selective sensitivity to bevacizumab (IC₅₀: 0.130 μM), doxorubicin (IC₅₀: 0.570 μM), carboplatin (IC₅₀: 0.950 μM), and topotecan (IC₅₀: 1.600 μM) in the 3D organoid model, with consistent cross-platform validation. An exploratory combination assay further supported enhanced viability suppression under bevacizumab-based regimens. Critically, at the time of manuscript preparation, the patient demonstrated radiological disease stabilization under bevacizumab plus trastuzumab deruxtecan, consistent with the organoid-derived response profile. These findings highlight the capacity of integrated genomic and organoid-based profiling to uncover therapeutic vulnerabilities beyond standard biomarker assessment. This proof-of-concept case report study demonstrates the feasibility and translational relevance of an established organoid-based functional precision oncology platform for therapeutic prioritization in metastatic rectal cancer. Full article

There is an important debate about the appropriate policy measures for reducing greenhouse gas (GHG) emissions in the transport sector. Strong expansion of battery electric vehicles (BEVs) following a ban on the registration of new vehicles with internal combustion engines (ICEs) by 2035 is a prominent but controversial proposal. To evaluate achievable GHG emission reductions, it is essential to understand the temporal dynamics of such a fleet transition. This study provides a time-resolved, policy-oriented quantification of annual and cumulative lifecycle GHG emissions during this process. Therefore, it uses an annual simulation model to assess GHG emissions from vehicle production and use during the transition of Germany’s passenger car fleet between 2019 and 2060. The analysis compares an ICE registration ban by 2035 with alternative scenarios and evaluates the effects of electricity decarbonization, greener BEV production, and the supply of additional Zero Emission Fuels (ZEFs). This study reveals a substantial time lag of 10–20 years between changes in new vehicle registrations and effective emission reductions. Even with a complete ICE ban by 2035, annual GHG emissions decline by only 3.7% by 2030 relative to 2025, while cumulative emissions over this period fall by just 1.6%. Larger reductions occur later, reaching 39% in 2040, 77% in 2050, and 82% in 2060 compared with 2025; cumulative emissions until 2060 decrease by 45%. Without an ICE ban and with a 75% BEV share from 2035 onward, cumulative reductions fall to 34%. Introducing additional ZEFs equivalent to 10% of 2030 fuel demand increases this value to 41%, compensating for much of the lower BEV uptake. Full article

Conifer-derived essential oils have gained attention as versatile natural additives with potential applications in animal production, including influencing microbial processes and supporting environmental sustainability. This study aimed to characterize the chemical composition of selected conifer essential oils (EOs), evaluate their antimicrobial activity against rumen microorganisms in vitro, and assess the effects of Pinus sylvestris essential oil on rumen fermentation and methane production under in vitro and in vivo conditions. EOs from Thuja occidentalis, Cupressus sempervirens, Juniperus communis, Picea mariana, Pinus sylvestris, and Pinus pinaster were analyzed by GC–MS, and their inhibitory activity against selected rumen bacteria was determined by MIC and IC₅₀ assays. Based on these results, P. sylvestris oil was selected for fermentation experiments. Ninety-two volatile compounds were identified, with monoterpenes as the dominant constituents and α-pinene as the major compound in P. sylvestris oil. In vitro, P. sylvestris oil influenced fermentation in a dose-dependent manner without affecting ruminal pH. In vivo, ruminal pH, ammonia-related parameters, and total VFA concentration were not significantly affected by treatment, whereas several variables showed a significant effect of time. Temporal changes in VFA profiles suggested a transient adaptation of ruminal fermentation. Methane concentration was significantly (p < 0.01) reduced by Pinus sylvestris essential oil supplementation, with a decrease of approximately 28.7% after 14 days. These findings indicate that P. sylvestris EOs may serve as a promising natural modulator of rumen fermentation, although further studies are needed to optimize dosage and confirm long-term effects. Full article

The demand for eco-friendly photoprotection has increased due to the observed adverse effects of conventional UV filters on marine ecosystems. In this study, we developed a broad-spectrum, reef-friendly sunscreen emulsion containing Perilla frutescens seed extract. The extraction process was optimized using 95% ethanol maceration for one week, yielding the highest SPF of 22.61. Gas chromatography–mass spectrometry (GC-MS/MS) was used to identify linolenic acid (43.54%) as the predominant fatty acid. Cytotoxicity test results for HaCaT keratinocytes were used to confirm the extract’s safety, with an IC₅₀ of 12.9 mg/mL. The formulated sunscreen met environmental safety standards based on persistence, bioaccumulation, and toxicity (PBT) criteria. A clinical safety evaluation using a 24 h closed patch test (n = 17) demonstrated that the formulation induced no significant alterations in TEWL or erythema levels (p > 0.05), confirming its dermatological safety. In a clinical efficacy evaluation involving 30 volunteers, the formulation containing 1% perilla extract reduced both erythema values and the melanin index, with no statistically significant difference observed (p > 0.05). These findings demonstrate that P. frutescens seed extract is a potent bioactive ingredient for sustainable cosmeceuticals, offering effective sun protection while ensuring safety for both human skin and marine environments. Full article

Hard carbon has been widely recognized as the most commercially viable anode material for sodium-ion batteries (SIBs); however, its inherently low initial Coulombic efficiency (ICE), typically 60–90%, remains a critical bottleneck constraining practical full-cell deployment. While extensive research has addressed ICE optimization, existing reviews have predominantly focused on individual precursor types or isolated strategies, lacking a unified cross-precursor comparative framework. This review systematically deconstructs the complete causal continua—from chemical composition through carbonization trajectories and microstructural evolution to ultimate ICE outcomes—across five major precursor categories: biomass, synthetic resins, pitches, coal-based materials, and saccharides. An “SSA-closed pore–defect” three-parameter trade-off framework is proposed to elucidate the microstructural origins of precursor-dependent ICE divergences. Cross-categorical benchmarking reveals that resin-based precursors achieve the highest ICE (95%) through ultra-low specific surface area and extensive closed porosity, pitch-based systems deliver the most consistent ICE distribution (86–91%), and coal-derived carbons are confined to the lowest tier (78–85%). The differentiated efficacy of carbonization conditions and post-treatment strategies across precursor types is critically evaluated, demonstrating that optimal process selection is inextricably linked to precursor taxonomy. Building upon these analyses, a precursor selection decision roadmap targeting three application-specific ICE thresholds is constructed, providing actionable guidance for matching precursor–process combinations to industrial requirements. The comparative framework is grounded in 25 representative studies selected through explicit inclusion criteria (detailed in the Introduction), and its predictive utility is illustrated for emerging precursor candidates beyond the five canonical categories. This cross-precursor perspective offers a systematic reference for accelerating the commercialization of hard carbon anodes in SIBs. Full article

Corn tortillas are consumed daily in Mexico. Alkaline extrusion is an alternative process that generates nixtamalized tortillas and preserves more bioactive compounds. Chickpea germination-extrusion may enhance the bioactive compound content. The aim was to characterize the physicochemical and antioxidant/antidiabetic properties of functional tortillas of alkaline-extruded blue corn (TC) with germinated (TG) or germinated-extruded (TGE) desi-chickpea. Likewise, the effect of simulated gastrointestinal digestion (SGD) on the bioaccessibility of bioactive compounds (phenolics, soluble protein, peptides, anthocyanins, and isoflavones) was estimated. Antioxidant capacity/cellular activity was determined by ORAC (AoxC) and in the Caco-2 cell line (CAA), while antidiabetic potential by α-amylase inhibition. The supplementation with processed chickpeas (TG/TGE) increased protein, ash, and isoflavone content (p < 0.05) compared with TC. SGD (%) released (p < 0.05) bioactive compounds from tortillas, and their bioaccessibility was among 34–70%; noticeably low phenolic bioaccessibility in TG/TGE. The AoxC was higher in TG/TGE (p < 0.05) compared with TC; in contrast, CAA was higher in TC, and both increased after SGD. TG depicted the lowest amylase inhibition; after SGD, the IC₅₀ values were 62–72-fold lower in the digests than in the tortillas. These results suggest that functional tortillas with processed chickpeas enhance nutraceutical potential. Full article

The crushing failure of sea ice is a critical design issue for polar offshore structures and ship structures because ice-induced loads may generate pronounced local damage and dynamic responses. Accurately modelling this process remains challenging because ice crushing involves localized fragmentation, crack propagation, rubble accumulation, and repeated contact release. This paper presents a controlled numerical sensitivity study of level-ice crushing against a vertical structure using a coupled LS-DYNA framework that combines the Structured Arbitrary Lagrangian–Eulerian (S-ALE) formulation with the Cohesive Element Method (CEM). The study focuses on a benchmark-scale indentation configuration and examines how mesh topology, mesh size, and imposed indentation velocity affect the predicted fracture morphology and load-time histories. The results show that random triangular meshes better reproduce stochastic fragmentation and lateral flaking than regular triangular or quadrilateral meshes, while finer meshes reduce excessive load oscillations and provide more stable force histories. The velocity study indicates a transition from gradual crushing and fragment retention at lower velocities to more rapid brittle chipping and stronger dynamic fluctuations at higher velocities. A benchmark-level comparison with published ice-indentation simulations shows that the predicted peak line load is of the same order of magnitude as reference results. The proposed framework is therefore useful for investigating numerical sensitivities and failure-mode trends in ice-crushing simulations, although final design-load application requires further calibration and formal mesh-independence assessment. Full article

This work presents the design and implementation of a flexible RFID tag based on a biocompatible and environmentally friendly conductive polymer, PEDOT:PSS, which is deposited onto a polyimide/fabric substrate using screen-printing techniques. The complete system consists of a dipole antenna based on PEDOT:PSS and a compact inductive metallic loop on a separate flexible printed circuit board (PCB) designed to match the capacitive impedance of a commercial RFID chip. The modular architecture, with the integrated circuit (IC) mounted on a reusable PCB substrate, shows efficient power transfer while allowing for easy disassembly, recycling, and consequently circularity of the PEDOT:PSS antenna and IC. By leveraging biocompatible materials and additive manufacturing processes, the proposed approach contributes to the advancement of sustainable and low-impact wireless technologies, addressing environmental concerns in next-generation electronics. Full article

Background/Objectives: Antimicrobial resistance, an evolutionarily entrenched microbial capacity amplified by extensive antibiotic exposure, has increased the burden of difficult-to-treat infections caused by priority pathogens such as Klebsiella pneumoniae, Pseudomonas aeruginosa and Staphylococcus aureus. In this study, we assessed whether phytochemical-rich extracts from fully ripe Rosa canina pseudo-fruits (WF) and fully developed Colchicum autumnale flowers (CA) can provide combined antioxidant, antibacterial, and antibiofilm effects against multidrug-resistant clinical isolates. Methods: Plant materials were processed using seven extraction systems spanning non-polar to polar conditions (n-hexane, ethyl acetate, n-butanol, aqueous, 40% ethanol, 60% ethanol, and enzyme-assisted hydrolysis). Fractions were quantified for total phenolics, flavonoids, and tannins, evaluated for antioxidant capacity (DPPH and FRAP), tested for antibacterial activity (disc diffusion and MIC/MBC), and assessed for inhibition of early biofilm attachment. Differences among extraction methods and fractions were analyzed using standard comparative statistics (group comparisons across solvents/fractions), and relationships between chemical composition and bioactivity were examined using correlation-based analysis. Results: Extraction strategy emerged as the main determinant of bioactivity across endpoints. The WFE/ENZ fraction maximized phytochemical recovery (TPC 203.34 ± 11.55 mg GAE/g DW; TFC 35.67 ± 3.06 mg QE/g DW; TTC 53.00 ± 2.65 mg TAE/g DW) and showed strong antioxidant performance (DPPH IC₅₀ 33.60 ± 0.02 μg/mL; FRAP A₇₀₀ 1.90 ± 0.010 at 250 μg/mL). Antibacterial effects were strongest in polar fractions, particularly hydroethanolic and enzyme-assisted extracts, while n-hexane fractions were consistently weakest. Across eight clinical isolates and three reference strains, MIC values ranged from 0.04875 to 6.25 mg/mL for WF extracts and 0.0975–12.5 mg/mL for CA extracts. In the biofilm model, suppression of early attachment was most consistent for CAE/E60–ENZ and WFE/E40–E60–ENZ fractions. Conclusions: Correlation analysis indicated that antibacterial potency aligned primarily with flavonoid levels in R. canina pseudo-fruits and with tannin content in C. autumnale material. Overall, these results support hydroethanolic and enzyme-assisted extraction as rational strategies to enrich polyphenol-dense fractions with convergent antioxidant, antibacterial, and antibiofilm activity, reinforcing plant-derived matrices as a structured discovery space for developing complementary antimicrobial solutions beyond conventional antibiotics. Notably, this is among the first studies to evaluate the antibacterial potential of C. autumnale plant material in this context and to comprehensively assess R. canina pseudo-fruit extracts against multidrug-resistant clinical. Full article

Goat blood, a major slaughterhouse by-product, was systematically valorized into dual-function bioactive peptides through an optimized four-step process. Four blood preparations—whole blood (HB), anticoagulant-treated blood (HBS), red blood corpuscles (BC), and plasma (PM)—were subjected to heat pretreatment (90 °C, 15 min) and enzymatic hydrolysis. Neutrase hydrolysis of heat-pretreated whole blood at 8% substrate concentration for 4 h (HBN-8) yielded optimal protein recovery (44.38%) with dual ACE (88.24%) and DPP-IV (81.13%) inhibition. Ultrafiltration enriched bioactive peptides in the ≤3 kDa fraction (DPP-IV: 87.8%; ACE: 65.5%). LC-MS/MS de novo sequencing identified 14 novel peptide sequences (4–9 amino acids), with the most potent SEC fraction showing IC₅₀ values of 0.89 and 0.45 mg Leu eq./mL for DPP-IV and ACE inhibition, respectively. Critically, simulated gastrointestinal digestion enhanced rather than diminished bioactivity, with ACE inhibition increasing progressively to 60.91% at the intestinal phase, supported by predicted generation of bioactive fragments from parent sequences. Caco-2 assays confirmed peptide safety (100–1000 µg/mL) and demonstrated 10.47% transepithelial transport with retained dual inhibitory activities. This study establishes goat blood as a sustainable source of orally bioavailable, GI-stable peptides for the development of functional foods targeting hypertension and type 2 diabetes. Full article