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Keywords = hydrodynamic size expansion

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25 pages, 5906 KB  
Article
Hydrodynamic Efficiency and Wake Interactions in Fish School Swimming
by Haoran Huang, Zhenming Yang, Junkai Liu, Jianhua Pang, Zongduo Wu, Hangyu Wen and Shunjun Li
Biomimetics 2026, 11(4), 278; https://doi.org/10.3390/biomimetics11040278 - 17 Apr 2026
Viewed by 777
Abstract
The mechanism by which fish enhance hydrodynamic performance through collective swimming is a research hotspot in the field of underwater bionic robots. This study employs the Immersed Boundary-Lattice Boltzmann Method (IB-LBM) to conduct numerical simulations on a two-dimensional, single-degree-of-freedom (1-DOF) autonomous propulsion bionic [...] Read more.
The mechanism by which fish enhance hydrodynamic performance through collective swimming is a research hotspot in the field of underwater bionic robots. This study employs the Immersed Boundary-Lattice Boltzmann Method (IB-LBM) to conduct numerical simulations on a two-dimensional, single-degree-of-freedom (1-DOF) autonomous propulsion bionic fish swarm. It systematically investigates the effects of swarm size and inter-individual spacing on swimming speed and cost of transport (CoT) under two typical configurations: series and parallel arrangements. Findings reveal that hydrodynamic benefits are highly dependent on the spatiotemporal evolution of flow field structures. In the series configuration, an optimal spacing range of 1.5 L to 2.0 L exists within the school, where the “wake capture” effect is pronounced. Trailing fish achieve a maximum speed increase of approximately 41.1% while significantly reducing energy consumption. However, as spacing increases to 2.5 L, the cooperative gain for front and middle-row individuals rapidly diminishes, and the lead fish even experiences significant performance loss. Uniquely, the trailing fish in the four-fish formation exhibits distinct flow field reorganization and performance recovery at the 4.5 L trailing position. In the parallel formation, the “channel effect” and “blocking effect” of the fluid dominate. The study identifies 0.4 L laterally as the critical instability spacing under the investigated kinematic regime, where strong destructive interference causes a sharp deterioration in individual swimming performance. Additionally, the parallel formation exhibits pronounced positional differentiation. Central individuals, constrained by dual lateral flow fields, experience restricted lateral wake expansion and accelerated energy dissipation, resulting in significantly weaker escape capabilities from low-speed conditions compared to marginal individuals. The vortex-dynamic mechanism revealed herein provides theoretical foundations for formation control in multi-fish biomimetic cooperative systems. Full article
(This article belongs to the Section Biomimetics of Materials and Structures)
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18 pages, 4322 KB  
Article
Experimental Investigation of the Local Scour Characteristics of Pipelines Crossing Rivers
by Qian Yang and Qinghua Yang
Water 2026, 18(7), 821; https://doi.org/10.3390/w18070821 - 30 Mar 2026
Viewed by 499
Abstract
Accurate prediction of burial depth and suspended length for oil and gas pipelines crossing rivers is critical for ensuring structural integrity. Systematic flume experiments were employed to examine local scour under varying hydrodynamic conditions, emphasizing relationships between scour hole expansion rate and flow [...] Read more.
Accurate prediction of burial depth and suspended length for oil and gas pipelines crossing rivers is critical for ensuring structural integrity. Systematic flume experiments were employed to examine local scour under varying hydrodynamic conditions, emphasizing relationships between scour hole expansion rate and flow velocity, water depth, and pipe diameter. Bedload transport predominantly governs riverbed evolution and scour hole development. Larger pipe diameters significantly reduce scour hole formation beneath the pipeline. Vertical expansion rate peaks immediately upon initial erosion, then progressively declines due to canalized flow, while cumulative scour depth continues increasing. Vertical dynamics at the pipe bottom conform to a first-order dynamic response equation, yielding a normalized time-dependent scour depth equation. Ultimate scour depth is collectively influenced by hydraulic parameters, pipe diameter, and sediment characteristics. Dimensionless correlations among scour depth, relative sediment size, and Froude number (Fr) were established via Gauss–Seidel iteration. Horizontal expansion exhibits distinct regimes: single-phase dominates at Fr > 0.6, whereas a secondary phase emerges at Fr ≤ 0.6. Integrating experimental data with empirical vertical expansion models, we propose a comprehensive horizontal scour expansion calculation model. These findings provide substantive insights into scour evolution mechanics and directly inform safety assessments for river-crossing pipelines. Full article
(This article belongs to the Section Hydraulics and Hydrodynamics)
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22 pages, 18934 KB  
Article
Morphological Controlling Factors of Braided River Reservoir Based on Delft3D Sedimentary Numerical Simulation: Application to Ordos Basin, China
by Jinbu Li, Kanglong Wang, Fuping Li, Zhixin Ma, Xinqiang Liu and Yuming Liu
Processes 2025, 13(11), 3661; https://doi.org/10.3390/pr13113661 - 12 Nov 2025
Cited by 1 | Viewed by 833
Abstract
To reveal the regulatory mechanisms and differences in sensitivity of hydrodynamic forces and sediment parameters to the sedimentary evolution of braided river channel bars, this study takes the Sulige Gas Field as a case study and conducts 21 sets of sedimentary numerical simulation [...] Read more.
To reveal the regulatory mechanisms and differences in sensitivity of hydrodynamic forces and sediment parameters to the sedimentary evolution of braided river channel bars, this study takes the Sulige Gas Field as a case study and conducts 21 sets of sedimentary numerical simulation experiments using the controlled variable method. The three parameters of discharge, slope gradient, and sediment grain size were fixed, while the target variable was adjusted iteratively. After the river reaches a steady state, quantitative statistics of the area and length-width ratio of 547 identified channel bars are carried out, and sensitivity evaluation is performed by combining principal component analysis and multiple linear regression. The results show that the sedimentary evolution of braided rivers follows a unified evolutionary law, the evolution of channel bars is synergistically regulated by parameter combinations. Under the action of single factors, an increase in discharge promotes the axial extension and scale expansion of channel bars; an increase in grain size enhances the morphological stability of channel bars; slope gradient controls the erosion-deposition balance through gravitational potential energy. The parameter sensitivity is ranked as slope gradient, discharge, sediment grain size. Full article
(This article belongs to the Special Issue Applications of Intelligent Models in the Petroleum Industry)
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15 pages, 2907 KB  
Article
Flexible Concentration Gradient Droplet Generation via Partitioning–Recombination in a Shear Flow-Driven Multilayer Microfluidic Chip
by Linkai Yu, Qingyang Feng, Yifan Chen, Yongji Wu, Haizhen Sun, Hao Yang and Lining Sun
Symmetry 2025, 17(6), 826; https://doi.org/10.3390/sym17060826 - 26 May 2025
Cited by 2 | Viewed by 1593
Abstract
Concentration gradient generation plays a pivotal role in advancing applications across drug screening, chemical synthesis, and biomolecular studies, yet conventional methods remain constrained by labor-intensive workflows, limited throughput, and inflexible gradient control. This study presents a novel multilayer microfluidic chip leveraging shear flow-driven [...] Read more.
Concentration gradient generation plays a pivotal role in advancing applications across drug screening, chemical synthesis, and biomolecular studies, yet conventional methods remain constrained by labor-intensive workflows, limited throughput, and inflexible gradient control. This study presents a novel multilayer microfluidic chip leveraging shear flow-driven partitioning–recombination mechanisms to enable the flexible and high-throughput generation of concentration gradient droplets. The chip integrates interactive upper and lower polydimethylsiloxane (PDMS) layers, where sequential fluid distribution and recombination are achieved through circular and radial channels while shear forces from the oil phase induce droplet formation. Numerical simulations validated the dynamic pressure-driven concentration gradient formation, demonstrating linear gradient profiles across multiple outlets under varied flow conditions. The experimental results revealed that the shear flow mode significantly enhances mixing uniformity and droplet generation efficiency compared to continuous flow operations, attributed to intensified interfacial interactions within contraction–expansion serpentine channels. By modulating hydrodynamic parameters such as aqueous- and oil-phase flow rates, this system achieved tunable gradient slopes and droplet sizes, underscoring the intrinsic relationship between flow dynamics and gradient formation. The proposed device eliminates reliance on complex channel networks, offering a compact and scalable platform for parallelized gradient generation. This work provides a robust framework for optimizing microfluidic-based concentration gradient systems, with broad implications for high-throughput screening, combinatorial chemistry, and precision biomolecular assays. Full article
(This article belongs to the Special Issue Symmetry/Asymmetry in Micro/Nanofluidic Devices and Applications)
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19 pages, 519 KB  
Article
Applicability of Hydrodynamics in the Hadronic Phase of Heavy-Ion Collisions
by Ronald Scaria, Captain R. Singh and Raghunath Sahoo
Physics 2025, 7(2), 13; https://doi.org/10.3390/physics7020013 - 18 Apr 2025
Viewed by 1763
Abstract
The hadronic phase and its dynamics in relativistic heavy-ion collisions are topics of immense discussion. The hadronic phase contains various massive hadrons with an abundance of the lightest hadron, i.e., π-mesons (pions). In this paper, we consider that pions are in the [...] Read more.
The hadronic phase and its dynamics in relativistic heavy-ion collisions are topics of immense discussion. The hadronic phase contains various massive hadrons with an abundance of the lightest hadron, i.e., π-mesons (pions). In this paper, we consider that pions are in the thermal equilibrium in the hadronic phase and use second-order viscous hydrodynamics for a medium of massive pions to obtain its expansion to the boundary of the kinetic freeze-out. We achieve the kinetic freeze-out boundary with the Knudsen number Kn>1 limit. When this condition is met, hydrodynamics expansion breaks down, and the mean free path becomes sufficiently large in comparison with the system size so that the particle yields are preserved. Further, we investigate the effect of the massive fluid on the resonance particle yields, including re-scattering and regeneration, along with the natural decay widths of the resonances. The resonances can play an essential role in determining the characteristics of the hadronic phase as they have sufficiently small lifetimes, which may be comparable to the hadronic phase lifetime. In the current study, we predict the hadronic phase lifetime, which is further used to determine the K*(892)0/K, ϕ(1020)/K, and ρ(770)0/π yield ratios at the kinetic freeze-out. We calculate these ratios as a function of charged particle multiplicity and transverse momentum and compare the findings with experimental data. Our calculations qualitatively agree with the experimental data, indicating a possible hydrodynamical evolution of the hadronic phase. Full article
(This article belongs to the Section High Energy Physics)
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25 pages, 19856 KB  
Article
Two-Dimensional Wave Interaction with a Rigid Body Floating near the Marginal Ice Zone
by Bingbing Wan, Yuyun Shi and Zhifu Li
J. Mar. Sci. Eng. 2024, 12(2), 272; https://doi.org/10.3390/jmse12020272 - 1 Feb 2024
Cited by 4 | Viewed by 1741
Abstract
The interaction problem of waves with a body floating near the marginal ice zone is studied, where the marginal ice zone is modeled as an array of multiple uniformly sized floating ice sheets. The linear velocity potential theory is applied for fluid flow, [...] Read more.
The interaction problem of waves with a body floating near the marginal ice zone is studied, where the marginal ice zone is modeled as an array of multiple uniformly sized floating ice sheets. The linear velocity potential theory is applied for fluid flow, and the thin elastic plate mode is utilized to describe the ice sheet deflection. A hybrid method is used to solve the disturbed velocity potential; i.e., around the floating body, a boundary integral equation is established, while in the domain covered by ice sheets, the velocity potential is expanded into an eigenfunction series, and in the far-field with a free surface, a similar eigenfunction expansion is used to satisfy the radiation condition. The boundary integral equation and the coefficients of the eigenfunction expansions are solved together based on the continuous conditions of pressure and velocity on the interface between the sub-domains. Extensive results for the equivalent Young’s modulus of the ice sheet array and hydrodynamic force on the body are provided, and the effect of individual ice sheet length as well as wave parameters are investigated in detail. Full article
(This article belongs to the Section Ocean Engineering)
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1836 KB  
Article
Evaluation of conventional fluid mechanic theory in small channels with singularity
by Sid Ali Si Salah, Abdelwahid Azzi and El Ghalia Filali
Int. J. Thermofluid Sci. Technol. 2023, 10(4), 100403; https://doi.org/10.36963/IJTST.2023100403 - 30 Nov 2023
Viewed by 70
Abstract
This study employs numerical simulations using the combined control volume finite element method (CVFEM) to analyze 2D steady, incompressible laminar flow over a microscale backward facing step within a horizontal duct. The investigation focuses on the impact of Reynolds number (Red) [...] Read more.
This study employs numerical simulations using the combined control volume finite element method (CVFEM) to analyze 2D steady, incompressible laminar flow over a microscale backward facing step within a horizontal duct. The investigation focuses on the impact of Reynolds number (Red) and expansion ratio (ER) on flow behavior, aiming to assess the applicability of conventional hydrodynamics at the microscale. The findings reveal that, for an expansion ratio of 2, the flow structure transforms progressively with varying Reynolds numbers in both laminar and transitional flow regimes. Notably, three recirculation zones develop downstream of the step, two along the lower wall and one along the upper wall. The primary recirculation zone's size expands as Reynolds number increases, contracting when a third recirculation zone emerges on the lower wall (Red ≥ 950). The study successfully matches its numerical predictions with experimental observations from larger-scale backward-facing steps for Reynolds numbers up to 500, maintaining two-dimensional flow characteristics. Furthermore, the computed velocity profiles align closely with experimental outcomes, except for Red = 1000, where the experimental flow shifts to three-dimensionality. The study also examines loss coefficients (Ke), revealing substantially higher values than conventional macro systems for Reynolds numbers above 200. However, for lower Reynolds numbers, the loss coefficient varies accordingly. For expansion ratios of 1.5, 2.0, and 2.5, fluid flow properties such as pressure, Poiseuille number, and friction factors exhibit good agreement with macroscale theory for fully developed laminar flow (Red ≤ 500). Full article
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11 pages, 2308 KB  
Article
An Innovative Sensor-Based Approach for Evaluating Performance of Flotation Circuit at the Expansion of Toromocho Copper Mine
by Wei Zhang, Zhiyong Tan, Tengfei Li, Xiaoqiang Guan, Shiqing Zhou, Haibin Li and Chao Wang
Processes 2023, 11(4), 1230; https://doi.org/10.3390/pr11041230 - 16 Apr 2023
Cited by 3 | Viewed by 2565
Abstract
Chinalco’s Toromocho mine, located in the Morococha district of Peru, treated 117,200 mtpd ores during last 10 years. It is currently undergoing expansion, treating approximately 52,740 mtpd of chalcopyrite ore since 2021. As with the commissioning of most large plants, the metallurgical performance [...] Read more.
Chinalco’s Toromocho mine, located in the Morococha district of Peru, treated 117,200 mtpd ores during last 10 years. It is currently undergoing expansion, treating approximately 52,740 mtpd of chalcopyrite ore since 2021. As with the commissioning of most large plants, the metallurgical performance levels produced after stability was achieved were below the design criteria; more specifically, the Cu overall recoveries were 80–82% compared with the design value of 85%, and the final concentrate Cu grades were 20–23% compared with the design value of 24% over the past 2 years. It is clear that the copper losses in the fine size fraction (<10 µm) were due to unoptimized hydrodynamics. To overcome this obstacle and improve overall performance, an innovative approach for flotation circuit evaluation was designed and set up, to improve understanding of the nature of the mineral losses, benchmark the cell hydrodynamics as a platform to improve flotation cell operation, and link the circuit setup and operation to gas dispersion characteristics. These needs were fulfilled by the developments in gas dispersion sensor technology and process measurement methodology of CRIST. Effectively utilizing the sensor technology, it was observed that the deficient recovery of the Toromocho expansion plant was due to poor recovery of <10 μm Cu, as a result of a lack of small bubbles (<1 mm) compared to the pilot circuit. The resulting data highlight the potential for recovery improvement (increased kinetics) via bubble size reduction and increased air rate. Full article
(This article belongs to the Special Issue Flow of Bubbles and Particles in the Flotation Process)
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14 pages, 3204 KB  
Communication
Facile Synthesis of Light-Switchable Polymers with Diazocine Units in the Main Chain
by Shuo Li, Katrin Bamberg, Yuzhou Lu, Frank D. Sönnichsen and Anne Staubitz
Polymers 2023, 15(5), 1306; https://doi.org/10.3390/polym15051306 - 5 Mar 2023
Cited by 9 | Viewed by 3631
Abstract
Unlike azobenzene, the photoisomerization behavior of its ethylene-bridged derivative, diazocine, has hardly been explored in synthetic polymers. In this communication, linear photoresponsive poly(thioether)s containing diazocine moieties in the polymer backbone with different spacer lengths are reported. They were synthesized in thiol-ene polyadditions between [...] Read more.
Unlike azobenzene, the photoisomerization behavior of its ethylene-bridged derivative, diazocine, has hardly been explored in synthetic polymers. In this communication, linear photoresponsive poly(thioether)s containing diazocine moieties in the polymer backbone with different spacer lengths are reported. They were synthesized in thiol-ene polyadditions between a diazocine diacrylate and 1,6-hexanedithiol. The diazocine units could be reversibly photoswitched between the (Z)- and (E)-configurations with light at 405 nm and 525 nm, respectively. Based on the chemical structure of the diazocine diacrylates, the resulting polymer chains differed in their thermal relaxation kinetics and molecular weights (7.4 vs. 43 kDa) but maintained a clearly visible photoswitchability in the solid state. Gel permeation chromatography (GPC) measurements indicated a hydrodynamic size expansion of the individual polymer coils as a result of the ZE pincer-like diazocine switching motion on a molecular scale. Our work establishes diazocine as an elongating actuator that can be used in macromolecular systems and smart materials. Full article
(This article belongs to the Special Issue Stimuli-Responsive Polymers)
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20 pages, 10474 KB  
Article
Design and Investigation of a 3D-Printed Micro-Fluidized Bed
by Yi Zhang, Kheng-Lim Goh, Yuen-Ling Ng, Yvonne Chow and Vladimir Zivkovic
ChemEngineering 2021, 5(3), 62; https://doi.org/10.3390/chemengineering5030062 - 13 Sep 2021
Cited by 10 | Viewed by 5453
Abstract
Micro-fluidized bed has aroused much attention due to its low-cost, intensified-process and fast-screening properties. In this paper, a micro-fluidized bed (15 × 15 mm in cross-section) was designed and fabricated with the use of the stereolithography printing technique, for the investigation of bubbles’ [...] Read more.
Micro-fluidized bed has aroused much attention due to its low-cost, intensified-process and fast-screening properties. In this paper, a micro-fluidized bed (15 × 15 mm in cross-section) was designed and fabricated with the use of the stereolithography printing technique, for the investigation of bubbles’ hydrodynamics and comparison of the solids (3D-printed particles VS fungal pellets) fluidization characteristics. In a liquid–gas system, bubble flow regime started from mono-dispersed homogeneous regime, followed by poly-dispersed homogeneous regime, transition bubble regime and heterogeneous bubble regime with increasing gas flowrates from 3.7 mL/min to 32.7 mL/min. The impacts from operating parameters such as gas flowrate, superficial liquid velocity and gas sparger size on bubble size, velocity and volume fraction have been summarized. In liquid–solid fluidization, different solid fluidization regimes for both particles bed and pellets bed were identified. From the bed expansion results, much higher Umf of 7.8 mm/s from pellets fluidization was observed compared that of 2.3 mm/s in particles fluidization, because the hyphal structures of fungal pellets increased surface friction but also tended to agglomerate. The similar R–Z exponent n (5.7 and 5.5 for pellets and particles, respectively) between pellets and particles was explained by the same solid diameter, but much higher Ut of 436 µm/s in particles bed than that of 196 µm/s in pellets bed is a consequence of the higher density of solid particles. This paper gives insights on the development of MFB and its potential in solid processing. Full article
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15 pages, 2876 KB  
Article
CFD Modeling and Simulation of the Hydrodynamics Characteristics of Coarse Coal Particles in a 3D Liquid-Solid Fluidized Bed
by Jian Peng, Wei Sun, Haisheng Han and Le Xie
Minerals 2021, 11(6), 569; https://doi.org/10.3390/min11060569 - 27 May 2021
Cited by 25 | Viewed by 4794
Abstract
In this study, a Eulerian-Eulerian liquid-solid two-phase flow model combined with kinetic theory of granular flow was established to study the hydrodynamic characteristics and fluidization behaviors of coarse coal particles in a 3D liquid-solid fluidized bed. First, grid independence analysis was conducted to [...] Read more.
In this study, a Eulerian-Eulerian liquid-solid two-phase flow model combined with kinetic theory of granular flow was established to study the hydrodynamic characteristics and fluidization behaviors of coarse coal particles in a 3D liquid-solid fluidized bed. First, grid independence analysis was conducted to select the appropriate grid model parameters. Then, the developed computational fluid dynamics (CFD) model was validated by comparing the experimental data and simulation results in terms of the expansion degree of low-density fine particles and high-density coarse particles at different superficial liquid velocities. The simulation results agreed well with the experimental data, thus validating the proposed CFD mathematical model. The effects of particle size and particle density on the homogeneous or heterogeneous fluidization behaviors were investigated. The simulation results indicate that low-density fine particles are easily fluidized, exhibiting a certain range of homogeneous expansion behaviors. For the large and heavy particles, inhomogeneity may occur throughout the bed, including water voids and velocity fluctuations. Full article
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19 pages, 5611 KB  
Article
Numerical Simulation of Effect of Different Initial Morphologies on Melt Hydrodynamics in Laser Polishing of Ti6Al4V
by Kai Li, Zhenyu Zhao, Houming Zhou, Hao Zhou, Jie Yin, Wei Zhang and Guiyao Zhou
Micromachines 2021, 12(5), 581; https://doi.org/10.3390/mi12050581 - 20 May 2021
Cited by 17 | Viewed by 3314
Abstract
As a surface finishing technique for rapid remelting and re-solidification, laser polishing can effectively eliminate the asperities so as to approach the feature size. Nevertheless, the polished surface quality is significantly sensitive to the processing parameters, especially with respect to melt hydrodynamics. In [...] Read more.
As a surface finishing technique for rapid remelting and re-solidification, laser polishing can effectively eliminate the asperities so as to approach the feature size. Nevertheless, the polished surface quality is significantly sensitive to the processing parameters, especially with respect to melt hydrodynamics. In this paper, a transient two-dimensional model was developed to demonstrate the molten flow behavior for different surface morphologies of the Ti6Al4V alloy. It is illustrated that the complex evolution of the melt hydrodynamics involving heat conduction, thermal convection, thermal radiation, melting and solidification during laser polishing. Results show that the uniformity of the distribution of surface peaks and valleys can improve the molten flow stability and obtain better smoothing effect. The high cooling rate of the molten pool resulting in a shortening of the molten lifetime, which prevents the peaks from being removed by capillary and thermocapillary forces. It is revealed that the mechanism of secondary roughness formation on polished surface. Moreover, the double spiral nest Marangoni convection extrudes the molten to the outsides. It results in the formation of expansion and depression, corresponding to nearby the starting position and at the edges of the polished surface. It is further found that the difference between the simulation and experimental depression depths is only about 2 μm. Correspondingly, the errors are approximately 8.3%, 14.3% and 13.3%, corresponding to Models 1, 2 and 3, respectively. The aforementioned results illustrated that the predicted surface profiles agree reasonably well with the experimentally measured surface height data. Full article
(This article belongs to the Special Issue Emerging Micro Manufacturing Technologies and Applications)
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14 pages, 43651 KB  
Article
Influence of High-Intensity Focused Ultrasound (HIFU) Ablation on Arteries: Ex Vivo Studies
by Yufeng Zhou and Wei Chun Daniel Lim
Micromachines 2021, 12(5), 485; https://doi.org/10.3390/mi12050485 - 25 Apr 2021
Cited by 7 | Viewed by 5266
Abstract
High-intensity focused ultrasound (HIFU) has been used to ablate solid tumors and cancers. Because of the hypervascular structure of the tumor and circulating blood inside it, the interaction between the HIFU burst and vessel is a critical issue in the clinical environment. Influences [...] Read more.
High-intensity focused ultrasound (HIFU) has been used to ablate solid tumors and cancers. Because of the hypervascular structure of the tumor and circulating blood inside it, the interaction between the HIFU burst and vessel is a critical issue in the clinical environment. Influences on lesion production and the potential of vessel rupture were investigated in this study for the efficiency and safety of clinical ablation. An extracted porcine artery was embedded in a transparent polyacrylamide gel phantom, with bovine serum albumin (BSA) as an indicator of the thermal lesion, and degassed water was driven through the artery sample. The HIFU focus was aligned to the anterior wall, middle of the artery, and posterior wall. After HIFU ablation, the produced lesion was photographically recorded, and then its size was quantified and compared with that in the gel phantom without artery. In addition, the bubble dynamics (i.e., generation, expansion, motion, and shrinkage of bubbles and their interaction with the artery) were captured using high-speed imaging. It was found that the presence of the artery resulted in a decrease in lesion size in both the axial and lateral directions. The characteristics of the lesion are dependent on the focus alignment. Acoustic and hydrodynamic cavitation play important roles in lesion production and interaction with the artery. Both thermal and mechanical effects were found on the surface of the artery wall after HIFU ablation. However, no vessel rupture was found in this ex vivo study. Full article
(This article belongs to the Special Issue Microbubbles for Ultrasound Therapy)
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12 pages, 4151 KB  
Article
Fluidization Dynamics of Hydrophobic Nanosilica with Velocity Step Changes
by Ebrahim H. Al-Ghurabi, Mohammad Asif, Nadavala Siva Kumar and Sher Afghan Khan
Appl. Sci. 2020, 10(22), 8127; https://doi.org/10.3390/app10228127 - 17 Nov 2020
Cited by 3 | Viewed by 2818
Abstract
Nanosilica is widely used in various applications, with its market expected to grow over USD 5 billion by 2025. The fluidized bed technology, owing to its intimate contact and efficient mixing of phases, is ideally suited for the large scale processing of powders. [...] Read more.
Nanosilica is widely used in various applications, with its market expected to grow over USD 5 billion by 2025. The fluidized bed technology, owing to its intimate contact and efficient mixing of phases, is ideally suited for the large scale processing of powders. However, the bulk processing and dispersion of ultrafine nanosilica using the fluidized bed technology are critically affected by the interparticle forces, such that the hydrophilic nanosilica shows agglomerate bubbling fluidization (ABF), while the hydrophobic nanosilica undergoes agglomerate particulate fluidization (APF). This study carried out a detailed investigation into the fluidization hydrodynamic of the hydrophobic nanosilica by monitoring the region-wise dynamics of the fluidized bed subjected to a regular step change of fixed duration in the gas velocity. The gas flow was controlled using a mass controller operated with an analog output signal from a data acquisition system. The analog input data were acquired at the sampling rate of 100 Hz and analyzed in both time and temporal frequency domains. The effect of velocity transients on the bed dynamics was quickly mitigated and appeared as lower frequency events, especially in regions away from the distributor. Despite the apparent particulate nature of the fluidization, strong hysteresis was observed in both pressure drop and bed expansion. Moreover, the fully fluidized bed’s pressure drop was less than 75% of the theoretical value even though the bed appeared to free from non-homogeneities. Key fluidization parameters, e.g., minimum fluidization velocity (Umf) and the agglomerate size, were evaluated, which can be readily used in the large scale processing of nanosilica powders using fluidized bed technology. Full article
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23 pages, 6956 KB  
Article
An Approach towards a GMP Compliant In-Vitro Expansion of Human Adipose Stem Cells for Autologous Therapies
by Valentin Jossen, Francesco Muoio, Stefano Panella, Yves Harder, Tiziano Tallone and Regine Eibl
Bioengineering 2020, 7(3), 77; https://doi.org/10.3390/bioengineering7030077 - 20 Jul 2020
Cited by 20 | Viewed by 6660
Abstract
Human Adipose Tissue Stem Cells (hASCs) are a valuable source of cells for clinical applications (e.g., treatment of acute myocardial infarction and inflammatory diseases), especially in the field of regenerative medicine. However, for autologous (patient-specific) and allogeneic (off-the-shelf) hASC-based therapies, in-vitro expansion is [...] Read more.
Human Adipose Tissue Stem Cells (hASCs) are a valuable source of cells for clinical applications (e.g., treatment of acute myocardial infarction and inflammatory diseases), especially in the field of regenerative medicine. However, for autologous (patient-specific) and allogeneic (off-the-shelf) hASC-based therapies, in-vitro expansion is necessary prior to the clinical application in order to achieve the required cell numbers. Safe, reproducible and economic in-vitro expansion of hASCs for autologous therapies is more problematic because the cell material changes for each treatment. Moreover, cell material is normally isolated from non-healthy or older patients, which further complicates successful in-vitro expansion. Hence, the goal of this study was to perform cell expansion studies with hASCs isolated from two different patients/donors (i.e., different ages and health statuses) under xeno- and serum-free conditions in static, planar (2D) and dynamically mixed (3D) cultivation systems. Our primary aim was I) to compare donor variability under in-vitro conditions and II) to develop and establish an unstructured, segregated growth model as a proof-of-concept study. Maximum cell densities of between 0.49 and 0.65 × 105 hASCs/cm2 were achieved for both donors in 2D and 3D cultivation systems. Cell growth under static and dynamically mixed conditions was comparable, which demonstrated that hydrodynamic stresses (P/V = 0.63 W/m3, τnt = 4.96 × 10−3 Pa) acting at Ns1u (49 rpm for 10 g/L) did not negatively affect cell growth, even under serum-free conditions. However, donor-dependent differences in the cell size were found, which resulted in significantly different maximum cell densities for each of the two donors. In both cases, stemness was well maintained under static 2D and dynamic 3D conditions, as long as the cells were not hyperconfluent. The optimal point for cell harvesting was identified as between cell densities of 0.41 and 0.56 × 105 hASCs/cm2 (end of exponential growth phase). The growth model delivered reliable predictions for cell growth, substrate consumption and metabolite production in both types of cultivation systems. Therefore, the model can be used as a basis for future investigations in order to develop a robust MC-based hASC production process for autologous therapies. Full article
(This article belongs to the Special Issue Stem Cell Bioprocessing and Manufacturing)
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