Search Results (524)

Heracleum sosnowskyi is considered to be a dangerous invasive plant species that has successfully naturalized within a variety of plant communities across numerous countries. As a result of its superior competitiveness, the alien species is able to displace the indigenous species from their native habitats, thus changing the ecosystems and decreasing biodiversity. The phytochemicals present in the H. sosnowskyi aqueous extracts were revealed using GC/MS and HPLC/DAD/TOF techniques. Isopsoralen, methoxsalen, (iso)pimpinellin and/or bergapten were determined to be major compounds in the leaf, inflorescence and root extracts. Glutaric, quinic, linolenic, (iso)chlorogenic and other polyphenolic acids were identified in the extracts. Furthermore, a number of furanocoumarins, including hermandiol, bakuchicin, candinols (A and C) and candibirin F, and coumarins, umbelliferone and yunngnins (A and B), were identified in the roots. Additionally, the presence of flavonoids, including astragalin, quercetin 7,3,4-trimethyl ether, nicotiflorin and rutin, has been detected in the flower and leaf extracts. Allelopathic effects of H. sosnowskyi aqueous extracts were tested on four model plants, lettuce (Lactuca sativa L.) and three native Lithuanian meadow herbs, perennial ryegrass (Lolium perenne L.), timothy (Phleum pratense L.) and white clover (Trifolium repens L.), using the Petri dish method. H. sosnowskyi flower and leaf extracts demonstrated the strongest inhibitory effects on the germination and growth of the tested plant seeds. At the highest relative concentrations, 0.5 and 1.0, extracts of Sosnowsky’s hogweed inflorescences inhibited timothy seedling growth by 95.47% (from 19.64 ± 2.57 mm to 0.89 ± 0.73 mm) and 100%, respectively. The leaf extracts exhibited the strongest inhibitory effects on white clover seedlings. The highest relative concentrations tested (0.5 and 1.0) suppressed clover seedling growth by 94.66% (from 41.22 ± 2.53 mm to 2.20 ± 0.63 mm) and 100%, respectively. Additionally, the germination rate and vigor index of model plants were assessed. The research is of significance for the regulation and monitoring of the spreading of aggressive H. sosnowskyi plants. Moreover, it is important for the development of natural herbicides based on active phytotoxic compounds from these plants. Full article

Weeds pose a significant threat to crop yields, both in quantitative and qualitative terms. Modern agriculture relies heavily on herbicides; however, their excessive use can lead to negative environmental impacts. As a result, recent research has increasingly focused on Integrated Weed Management (IWM), which employs multiple complementary strategies to control weeds in a holistic manner. Nevertheless, large-scale adoption of this approach requires a solid understanding of the underlying tactics. This systematic review analyses recent studies (2013–2022) on herbicide alternatives for weed control across major cropping systems in the EU-27 and the UK, providing an overview of current knowledge, the extent to which IWM tactics have been investigated, and the main gaps that help define future research priorities. The review relied on the IWMPRAISE framework, which classifies weed control tactics into five pillars (direct control, field and soil management, cultivar choice and crop establishment, diverse cropping systems, and monitoring and evaluation) and used Scopus as a scientific database. The search yielded a total of 666 entries, and the most represented pillars were Direct Control (193), Diverse Cropping System (183), and Field and Soil Management (172). The type of crop most frequently studied was arable crops (450), and the macro-area where the studies were mostly conducted was Southern Europe (268). The tactics with the highest number of entries were Tillage Type and Cultivation Depth (110), Cover Crops (82), and Biological Control (72), while those with the lowest numbers were Seed Vigor (2) and Sowing Depth (2). Overall, this review identifies research gaps and sets priorities to boost IWM adoption, leading policy and funding to expand sustainable weed management across Europe. Full article

Weed management has become a critical agricultural practice, as weeds compete with crops for nutrients, host pests and diseases, and cause major economic losses. The invasive weed Ambrosia artemisiifolia (common ragweed) is particularly problematic in Hungary, endangering crop productivity and public health through its fast proliferation and allergenic pollen. This review examines the current challenges and impacts of A. artemisiifolia while exploring sustainable approaches to its management through precision agriculture. Recent advancements in unmanned aerial vehicles (UAVs) equipped with advanced imaging systems, remote sensing, and artificial intelligence, particularly deep learning models such as convolutional neural networks (CNNs) and Support Vector Machines (SVMs), enable accurate detection, mapping, and classification of weed infestations. These technologies facilitate site-specific weed management (SSWM) by optimizing herbicide application, reducing chemical inputs, and minimizing environmental impacts. The results of recent studies demonstrate the high potential of UAV-based monitoring for real-time, data-driven weed management. The review concludes that integrating UAV and AI technologies into weed management offers a sustainable, cost-effective, mitigate the socioeconomic impacts and environmentally responsible solution, emphasizing the need for collaboration between agricultural researchers and technology developers to enhance precision agriculture practices in Hungary. Full article

Urinary bladder neoplasms are clinically relevant in dogs and cats and are also common in humans, all of which may share exposure to environmental factors that influence disease risk. In Veterinary Medicine, however, their etiological determinants remain poorly defined. Urinary bladder neoplasia range from non-invasive lesions limited to the mucosa to invasive forms that infiltrate the muscular layer, which are more aggressive and metastatic. In dogs, invasive urothelial carcinoma (UC) represents the most frequently diagnosed type, while in cats, it is less common but displays similar biological behavior. Hematuria and dysuria are the predominant clinical signs, and although urinary bladder cancer accounts for only a small proportion of canine neoplasms, it is associated with considerable morbidity and mortality. Several risk factors have been identified, including breed, sex, age, obesity, diet, neuter status, and environmental exposures. Female dogs, especially Terrier breeds, are more susceptible, whereas in cats, males and short-haired animals are more often affected. Contact with insecticides, herbicides, and antiparasitic products is a recognized risk factor in dogs, although this association has not been consistently demonstrated in cats. Neutering and obesity appear to increase risk in dogs, and dietary patterns may offer protection, with regular vegetable consumption linked to a reduced incidence. Understanding these determinants is essential to improve early detection, guide preventive measures, and strengthen comparative oncology research. Full article

Background: Gestational diabetes mellitus (GDM) is a frequent pregnancy pathology with poor maternal and fetal outcomes and risk of type 2 diabetes in later life. Despite known risk factors, such as obesity, age, and familial history, new data suggest that environmental exposure to agents, such as pesticides, can play a role in the etiogenesis of GDM. Objective: The epidemiologic, experimental, and mechanistic evidence between pesticide exposure and GDM risk is summarized here, and we concentrate on recent research (2000–2025). Methods: We conducted a literature search in PubMed, Embase, and the Cochrane Library for studies published from January 2000 to December 2025 using combinations of the terms “fertilizers”, “herbicides”, and “pesticides” with “diabetes mellitus” and “gestational diabetes”. After deduplication, 12 unique studies met inclusion criteria for qualitative synthesis (GDM endpoint or pregnancy glycemia with pesticide exposure). Results: Occupational and agricultural exposure to pesticides during first pregnancy was determined to be associated with a significantly increased risk of GDM through various epidemiologic studies. New studies have implicated new classes of pesticides, including pyrethroids and neonicotinoids, with higher GDM risk with first-trimester exposure. Experimental studies suggest that pesticides provide potential endocrine-disrupting chemicals that can induce insulin resistance through disruption of hormonal signaling, oxidative stress, inflammation, β-cell toxicity, and epigenetic modifications. However, significant limitations exist. Most of the evidence is observational, measurement of exposure is often indirect, and confounding factors are difficult to exclude. Notably, low dietary and residential exposure is not well studied, and dose–response relationships are undefined. Conclusions: New data indicate that pesticide exposure during early pregnancy and occupational exposure may increase the risk of GDM. Prospective cohort studies using biomonitoring, chemical mixture exposure, and geographic variation in pesticide exposure should be the focus of future research. Due to potential public health implications, preventive strategies to ensure the quality of nutrition and to reduce maternal exposure to pesticides during pregnancy are rational. Full article

The widespread use of glyphosate-based herbicides (GBHs) has raised concerns about their potential role in hormone-sensitive cancers such as breast cancer. This systematic review aimed to evaluate preclinical evidence on the effects of glyphosate (pure compound) or glyphosate-based herbicide formulations (GBHs) exposure on breast cancer cell proliferation and related molecular pathways. A structured search was conducted across PubMed, ScienceDirect, and Springer Nature Link, Web of Science databases, covering studies published up to 9 November 2025, following a PROSPERO-registered protocol (ID: CRD42021238350). Eligible studies included original in vitro and in vivo preclinical research using human breast cancer cell lines (e.g., MCF-7, T47D, MDA-MB-231, MCF-12A, and MCF-10A) or relevant animal models. Outcomes assessed included cell viability, proliferation, tumor growth, apoptosis, cell cycle regulation, and molecular markers associated with endocrine signaling. Two reviewers independently screened and extracted data, resolving disagreements via discussion or third-party adjudication. From an initial pool of 699 articles, seven in vitro studies met the inclusion and quality criteria. Glyphosate exposure demonstrated weak estrogenic activity in ER-positive breast cancer cells, primarily via ERα modulation and altered gene expression related to proliferation and DNA repair. GBHs showed greater cytotoxic and epigenetic effects in non-tumorigenic cells, often independent of ER signaling. No included study employed in vivo breast cancer models. Overall, preclinical evidence suggests glyphosate may act as a weak endocrine disruptor under specific conditions, but findings are limited by the short-term in vitro designs, heterogeneous methodologies, and lack of chronic or in vivo data. Further research using long-term exposure and animal models is needed to clarify potential risks and inform regulatory and public health decisions. Full article

Oats are regaining interest because of their nutritional and agro-environmental benefits. Hence, research into increasing oat productivity through sustainable agronomic practices has become increasingly important, especially as new varieties are developed and weather patterns become more unpredictable. The paper presents the effects of the cropping system (six-field crop rotation, continuous cropping since 1968), variety (two per six-year period), chemical crop protection (control, herbicide, herbicide plus fungicide), and study year, on spring oat grain yields for two six-year crop rotation cycles (2011–2016, 2017–2022) of a long-term experiment in Poland. The cropping system was the most influential factor. Studies confirmed that growing oats in crop rotation ensures higher productivity than continuous cropping and sustains satisfactory yields in Polish conditions despite yearly weather variability. The cultivated varieties differed in yield levels and degree of yield reduction in response to continuous oat cropping. Only during the 2011–2016 cycle was a decreasing trend in yields observed as continuous cropping was prolonged. Oats grown in crop rotation rarely benefited from chemical protection against weeds and pathogens. In continuous cropping, herbicide and fungicide treatments typically did not mitigate oat yield losses associated with the system, exacerbating them in the 2017–2022 cycle. Among the evaluated agronomic practices, the six-field crop rotation system proved the most reliable yield-enhancing strategy, whereas chemical protection rarely improved oat performance. In individual years, contradictory reactions of the two cultivated varieties to cropping systems and crop protection levels were often noted. Full article

The invasive plant Sphagneticola trilobata (Asteraceae), known for its rapid growth and strong adaptability, has spread widely across tropical and subtropical regions worldwide, posing a serious threat to local plant diversity. Traditional weed control approaches have limited effectiveness, and the overuse of chemical herbicides such as glyphosate not only leads to resistance but also harms the environment. This study elucidated the important roles of jasmonic acid (JA) and arbuscular mycorrhizal fungi (AMF) in jointly promoting the herbicide resistance of S. trilobata. Firstly, the herbicide tolerance of S. trilobata was tested. Then, a field experiment was conducted to test the relation between AMF colonization and herbicide resistance in S. trilobata by high-throughput sequencing, and the metabolomics analysis was conducted to test the secondary metabolite difference by AMF colonization. Lastly, a greenhouse experiment was conducted to assess the synergistic effects of JA and AMF on S. trilobata’s herbicide resistance. Results showed that invasive S. trilobata has stronger glyphosate tolerance than its native congener. The field experiment showed that glyphosate treatment significantly increased the AMF colonization in S. trilobata and altered the composition of the rhizosphere AMF community. Metabolomics analysis revealed that AMF colonization upregulates the expression of stress-related metabolites, especially JA content. The greenhouse experiment further validated that both AMF colonization and JA application could enhance the stem and root length and plant biomass. Under glyphosate application, AMF and JA enhanced plant growth and relative chlorophyll content, while reducing relative flavonol and anthocyanin contents. Furthermore, the interaction of AMF and JA treatments led to a significant synergistic effect in plant growth and resistance to glyphosate. Our findings emphasize the necessity to simultaneously consider eliminating the promoting effects of JA and AMF on the herbicide resistance when implementing chemical control management strategies for the control of S. trilobata. This study provides new theoretical bases and sustainable control strategies for invasive plant management, as well as important references for research on plant-microbe interactions and stress resistance mechanisms. Full article

To address the issue of improving the accuracy and efficiency of variable-rate application under large-scale field conditions, an RBFNN-SMC variable-rate application control system was designed and experimentally verified. A first-order inertial pure lag model of the spray flow rate was identified through step tests. Based on this, three controllers, namely traditional SMC, fuzzy SMC, and RBFNN-SMC, were designed for comparison. The simulation results indicated that RBFNN-SMC had the shortest adjustment time and the smallest steady-state error under conditions of model uncertainty and time delay. Its maximum overshoot was reduced by 11.2% compared to traditional SMC, and the steady-state error was controlled within ±0.51%. The control strategy was implemented on a microcontroller and integrated with a prescription-based spray system. Field trials showed that the average absolute error of the proposed system in tracking the target flow rate was 3.2%, and it achieved a weed control effect comparable to that of traditional uniform spraying. These results suggest that the RBFNN-SMC-based variable spray rate system can enhance flow rate control performance and support more precise and potentially more sustainable herbicide application, providing a reference and ideas for the research on variable-rate application flow rate control based on prescription maps. Full article

Chemical control of weeds with the herbicide glyphosate under vines in the vineyards is currently easy, effective, and cheap. There are currently no completely equivalent alternative herbicides or suitable mechanical control methods that have the same efficacy in suppressing weeds under vines in vineyards as glyphosate. Therefore, in this research, we tested two alternative technologies for controlling weeds under the vines as a counterweight to the predominant control approach with the herbicide glyphosate: (1) chemical control with pelargonic acid, acetic acid, and the plant extract-based fertilizer Stopeco^® with herbicidal action, and (2) mechanical control with a combined tool consisting of a rotary star tiller and finger weeder. A comparative analysis was conducted on time and fuel consumption, the extent of the carbon footprint, grape yield, and quality, which showed that the tested alternative methods of weed control were not comparable to the herbicide glyphosate in terms of effectiveness in weed suppression but were comparable at grape yield. In our trial, at the number of treatments we performed, differences in environmental footprint between different treatments were significant (glyphosate variant 10.55–11.21 gha anno⁻¹; other variants 7.48–8.08 gha anno⁻¹). Alternative mechanical and chemical methods need to be applied at least three to four times a year to achieve results comparable to those from two applications of glyphosate. For this reason, it is possible that, in the case of a slightly increased number of passes by mechanical tools or a slightly increased number of sprayings with alternative preparations to reach the efficacy level of glyphosate treatments, the foot print parameter, CO₂ emissions and global warming potential (GWP) parameter in alternative treatments would no longer be more favorable than when using the herbicide glyphosate twice a year. Full article

Glyphosate, a widely used non-selective herbicide, has been a subject of intense scientific debate due to its environmental persistence and potential health risks. This review examines glyphosate’s mechanisms of action, its effects on crop production, and its broader environmental impact, including soil degradation, water contamination, and biodiversity loss. Furthermore, it examines the expanding body of research linking glyphosate exposure to various human health concerns, including metabolic, neurological, reproductive, and oncological disorders. The review also assesses glyphosate’s role in hindering the achievement of the Sustainable Development Goals (SDGs), particularly those related to food security, health, access to clean water, and the protection of marine ecosystems. Finally, potential alternatives to glyphosate-based weed control, including organic and non-chemical methods, are discussed to promote sustainable agricultural practices that balance productivity with ecological and public health considerations. The evidence reviewed highlights glyphosate’s pervasive presence across ecosystems and its potential to disrupt both environmental and human health. The findings underscore the urgent need to regulate glyphosate use, prioritize soil and water protection, and accelerate the transition toward sustainable, low-toxicity weed management strategies that align with global sustainability objectives. Full article

Weeds are among the primary constraints reducing soybean productivity, and their effective control is especially important in edamame, a vegetable soybean valued for its nutritional potential. As chemical control remains the dominant strategy, rational herbicide use is essential. This study aimed to evaluate the response of two edamame varieties (Chiba Green and Midori Giant) and the effectiveness of applied herbicides in weed control during the 2022–2024 growing seasons. Treatments included the following: pre-emergence herbicides (S-metolachlor + metribuzin) (H1); pre- (S-metolachlor + metribuzin) and post-emergence herbicides (imazamox + cycloxydim) (H2); and an untreated control (H0). The growing season influenced pod yield and biomass, with the highest yield recorded in 2022 (11.7 t ha⁻¹), while variety affected only pod yield: on average, Midori Giant outperformed Chiba Green (10.6 vs. 6.1 t ha⁻¹). Herbicide treatment affected weed dry biomass (3.3 g m⁻² in H2 compared to 341.8 g m⁻² in H0) and pod yield (4.3 t ha⁻¹ in H0 for Chiba Green compared to 11.9 t ha⁻¹ in H2 for Midori Giant). The results indicate that pre-emergence herbicides could satisfactorily reduce weed infestation under suitable meteorological conditions. The combined application of pre- and post-emergence herbicides increases production security (particularly in seasons with higher weed infestation), likely by extending the weed control period through pre- and post-emergence herbicide combinations, targeting different weed species during the soybean vegetative period. In addition, weed diversity was associated with a yield increase in Midori Giant. This research provides practical information and options for weed management in edamame production in the Western Balkan region. Full article

The problem of water pollution by various xenobiotics has gained a lot of interest due to their persistence, bioaccumulation potential, and toxic effects on ecosystems and humans. Electrochemical sensors offer a rapid, sensitive, and cost-effective method for on-site monitoring. In this research, an electrochemical sensor for xenobiotics based on a biochar–alumina composite is developed. The biochar–alumina composites were obtained by the air-limited pyrolysis of oak sawdust in the presence of alumina. Two types of alumina were mixed with oak sawdust in three ratios and subjected to thermal treatment. The resulting composites were characterized by SEM, N₂ adsorption isotherm, XRD, and electrochemical characterization. The detection of the herbicide pendimethalin and the antibiotic ciprofloxacin was investigated, and the composite with the optimal biochar/alumina ratio was selected for each of the xenobiotics studied. A linear current response was obtained for pendimethalin in the concentration range 0.7 μM to 70.0 μM with an LOD of 0.5 μM. A linear current response was obtained for ciprofloxacin in the concentration range 1.6 μM to 55.4 μM with an LOD of 0.63 μM. A comparison of the characterization results with the electroanalytical performance implied the importance of the hydrophobic/hydrophilic nature of the electrode surface for detecting the analyte under investigation. Full article

Weed management is always a challenge in crop production, exacerbated by the issue of herbicide resistance. Excessive herbicide application not only leads to the development of herbicide resistance weeds but also causes environmental problems. In precision agriculture, innovative weed management methods, especially advanced remote sensing and computer vision technologies for targeted herbicide applications, i.e., site-specific weed management (SSWM), have recently drawn a lot of attention. Challenges exist in accurately and reliably detecting diverse weed species under varying field conditions. Significant efforts have been made to advance computer vision technologies for weed detection. This comprehensive review provides an in-depth examination of various methodologies used in developing weed detection systems. These methodologies encompass a spectrum ranging from traditional image processing techniques to state-of-the-art machine and deep learning models. The review further discusses the potential of these methods for real-time applications, highlighting recent innovations, and identifying future research hotspots in SSWM. These advancements hold great promise for further enhancing and innovating weed management practices in precision agriculture. Full article

2,4-Dichlorophenoxyacetic acid (2,4-D) is a widely used herbicide, yet its potential to induce hepatorenal injury via oxidative stress and apoptosis raises significant health concerns. Lycium barbarum polysaccharides (LBP) possess recognized antioxidant and anti-apoptotic properties, but their protective mechanisms against 2,4-D toxicity, particularly through a multi-target network, remain inadequately explored. This study aimed to systematically investigate the mechanisms of 2,4-D-induced hepatorenal injury and the protective efficacy of LBP by integrating network toxicology, molecular docking, and experimental validation. An integrated approach was employed. Core targets and pathways were identified via network toxicology. Molecular docking predicted interactions between 2,4-D and these targets. In vivo validation was conducted on Sprague-Dawley rats treated with 2,4-D (75 mg/kg) and/or LBP (50 mg/kg) for 28 days, assessing histopathology, serum oxidative stress markers superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), malondialdehyde (MDA) and cellular apoptosis (TUNEL staining). Network analysis identified PPARG, NFKB1, PPARA, NFE2L2, and SERPINE1 as core targets, with molecular docking confirming strong binding affinities (binding energies: −5.1 to −6.3 kcal·mol⁻¹) and KEGG enrichment implicating cAMP, Ca²⁺, and PPAR signaling pathways. Experimentally, 2,4-D exposure induced significant histopathological damage, suppressed SOD/GSH-Px activities (p < 0.001), elevated MDA levels (p < 0.001), and markedly increased renal apoptosis (p < 0.01). Crucially, LBP intervention substantially mitigated these alterations, ameliorating tissue injury, restoring antioxidant defenses, increasing SOD/GSH-Px (p < 0.01), reducing MDA (p < 0.001) and significantly decreasing renal apoptosis (p < 0.05). This study elucidates a multi-target mechanism for 2,4-D-induced hepatorenal injury centered on oxidative stress–apoptosis dysregulation and demonstrates that LBP confers significant protection likely via modulation of this network. These findings underscore the potential of LBP as a natural protective agent against pesticide-induced organ damage and highlight the utility of integrated network approaches in toxicological research. Full article