Search Results (372)

Colibacillosis is a poultry disease caused by the pathogenic bacterium Escherichia coli (E. coli). This study is an experimental cross-sectional study using herbal-based test materials from Javanese cardamom and turmeric rhizome as treatments to replace the role of antibiotics that experience drug resistance in several types of bacteria. A total of 32 samples were utilized in this study, separated into two control groups and six treatment groups. The analysis was carried out by an histopathological examination of the lung organs using H&E and ImageJ staining to calculate the area of the slide image. The data results were analyzed statistically with one-way ANOVA method and qualitatively. The outcome of the statistical test showed that the differences were not statistically significant p value = 0.922 [p > 0.05] in all groups, and findings from qualitative histopathology showed morphological differences in the alveoli, parabronchi, and vasculature in the lung organs. Full article

In recent years, most of the populations of the world have been using herbal materials for their strong antimicrobial properties and major health benefits. The objective of this study was to evaluate the phenolic profile, as well as the antioxidant and antimicrobial activities, of a dietary supplement composed of extracts from hibiscus (Hibiscus sabdariffa L.) calyces and lemon verbena (Lippia citriodora) leaves (Metabolaid^®, Patent P201731147) mixed at a weight ratio of 35:65 (w/w), respectively. The bioactive components of the methanolic extract were analyzed by UHPLC-ESI-MS/MS. The antioxidant activity was evaluated using spectrophotometric methods, while the antimicrobial activity was assessed through the microdilution method against selected Gram-negative and Gram-positive bacteria. The total phenols content resulted in being 256.10 ± 2.26 mg GAE/g f.w., the flavonoid content was 48.90 ± 2.95 mg CE/g f.w., flavonols were 60.17 ± 7.68 mg QE/g f.w., and anthocyanins were 3.78 ± 0.17 mg C3GE/g f.w. The FRAP value, observed in the natural mix additive, was 1.25 ± 0.03 mg Fe²⁺/g f.w., while the ORAC showed the value of 1893.77 ± 30.39 µmol TE/g f.w. and the DPPH was 23.33 ± 4.12 µg/mL. We found eight phenolic acids, seven flavonols, five anthocyanins, and nine other phenolic compounds. The extract showed a minimum inhibitory concentration (MIC) of 12.5 mg/mL against E. coli, E. aerogenes, and E. faecalis and of 25 mg/mL against S. enterica ser. Typhimurium and S. aureus and a minimum bactericidal concentration (MBC) of 25 mg/mL against E. coli, E. aerogenes, and E. faecalis and of 50 mg/mL against S. enterica ser. Typhimurium and S. aureus. In conclusion, our findings demonstrate that Metabolaid^® is a rich source of bioactive compounds and provides beneficial effects against oxidative stress and pathogenic bacteria, supporting its nutraceutical potential. Full article

The root of Plumbago indica L. is commercially available in herbal markets in both crude and powdered forms. P. indica root is a key ingredient in numerous polyherbal formulations. However, P. indica has two closely related species, P. zeylanica L. and P. auriculata Lam. Since only P. indica is traditionally used in Thai polyherbal products, adulteration with other species could potentially compromise the therapeutic efficacy and overall effectiveness of these formulations. To address this issue, a MassARRAY iPLEX assay was developed to accurately identify and differentiate P. indica from its closely related species. Five single nucleotide polymorphism (SNP) sites—positions 18, 112, 577, 623, and 652—within the internal transcribed spacer (ITS) region were selected as genetic markers for species identification. The assay demonstrated high accuracy in identifying P. indica and was capable of detecting the species at DNA concentrations as low as 0.01 ng/µL. Additionally, the assay successfully identified P. zeylanica in commercial crude drug samples, highlighting potential instances of adulteration. Furthermore, it was able to distinguish P. indica in mixed samples containing P. indica, along with either P. zeylanica or P. auriculata. The developed MassARRAY iPLEX assay proves to be a reliable and effective molecular tool for authenticating P. indica raw materials. Its application holds significant potential for ensuring the integrity of herbal products by preventing misidentification and adulteration. Full article

This study presents research on the production of activated biocarbons derived from herbal waste. Sage stems were chemically activated with two activating agents of different chemical natures—H₃PO₄ and K₂CO₃—and subjected to two thermal treatment methods: conventional and microwave heating. The effect of the activating agent type and heating method on the basic physicochemical properties of the resulting activated biocarbons was investigated. These properties included surface morphology, elemental composition, ash content, pH of aqueous extracts, the content and nature of surface functional groups, points of zero charge, and isoelectric points, as well as the type of porous structure formed. In addition, the potential of the prepared carbonaceous materials as adsorbents of model organic (represented by Triton X-100 and methylene blue) and inorganic (represented by iodine) pollutants was assessed. The influence of the initial adsorbate concentration (5–150 (dye) and 10–800 mg/dm³ (surfactant)), temperature (20–40 °C), and pH (2–10) of the system on the efficiency of contaminant removal from aqueous solutions was evaluated. The adsorption kinetics were also investigated to better understand the rate and mechanism of contaminant uptake by the prepared activated biocarbons. The results showed that materials activated with orthophosphoric acid exhibited a significantly higher sorption capacity for all tested adsorbates compared to their potassium carbonate-activated counterparts. Microwave heating was found to be more effective in promoting the formation of a well-developed specific surface area (471–1151 m²/g) and porous structure (mean pore size 2.17–3.84 nm), which directly enhanced the sorption capacity of both organic and inorganic contaminants. The maximum adsorption capacities for iodine, methylene blue, and Triton X-100 reached the levels of 927.0, 298.4, and 644.3 mg/g, respectively, on the surface of the H₃PO₄-activated sample obtained by microwave heating. It was confirmed that the heating method used during the activation step plays a key role in determining the physicochemical properties and sorption efficiency of activated biocarbons. Full article

Background: Allergic rhinitis (AR) is a prevalent allergic disorder characterized by a complex pathogenesis. Drawing on traditional Chinese medicine theory and contemporary pharmacological principles, this study developed an inhalation-based herbal formulation, ZHW, to explore a novel non-invasive therapeutic approach. Objective: To investigate the therapeutic effects of ZHW on AR and elucidate its underlying mechanisms and potential targets through an integrated analysis of network pharmacology and proteomics. Materials and Methods: The volatile components of ZHW were analyzed by gas chromatography–mass spectrometry (GC-MS). The mouse model of AR was induced by OVA sensitization. The therapeutic efficacy of ZHW was assessed based on nasal symptom scores, histopathological examination, and inflammatory cytokine levels. Furthermore, the underlying mechanisms and potential targets of ZHW were investigated through integrated network pharmacology and proteomics analyses. Results: GC-MS analysis identified 39 bioactive compounds in ZHW. Inhalation treatment with ZHW demonstrated significant anti-allergic effects in OVA-sensitized mice, as evidenced by (1) reduced sneezing frequency and nasal rubbing behaviors; (2) decreased serum levels of IL-4, histamine, and OVA-specific IgE; (3) attenuated IL-4 concentrations in both nasal lavage fluid and lung tissue; (4) diminished nasal mucosal thickening; and (5) suppression of inflammatory cell infiltration. Integrated network pharmacology and proteomics analyses indicated that ZHW’s therapeutic effects were mediated through the modulation of multiple pathways, including the PI3K-Akt signaling pathway, the B cell receptor signaling pathway, oxidative phosphorylation, and the FcεRI signaling pathway. Key molecular targets involved Rac1, MAPK1, and SYK. Molecular docking simulations revealed strong binding affinities between ZHW’s primary bioactive constituents (linalool, levomenthol, linoleic acid, Linoelaidic acid, and n-Valeric acid cis-3-hexenyl ester) and these target proteins. Conclusions: The herbal formulation ZHW demonstrates significant efficacy in alleviating allergic rhinitis symptoms through multi-target modulation of key signaling pathways, including PI3K-Akt- and FcεRI-mediated inflammatory responses. These findings substantiate ZHW’s therapeutic potential as a novel, non-invasive treatment for AR and provide a strong basis for the development of new AR therapies. Future clinical development will require systematic safety evaluation to ensure optimal therapeutic outcomes. Full article

The aim of this study was to determine the intraspecific variability among 11 wild-growing populations of wormwood (Artemisia absinthium L.) originating from Central Europe and preserved in the Polish Genebank Collection. The populations were introduced into ex situ conditions, and assessed in terms of selected developmental and chemical traits (essential oil, phenolic acids, polyphenols, and tannins content). Developmental observations and harvest of raw materials were carried out in the second year of plant vegetation, at the beginning of flowering. The populations exhibited significant differences. The greatest variability was observed in the number of shoots per plant (38–51) and dry mass of herb per plant (0.83–1.60 kg). Essential oil (EO) content ranged from 0.75 to 1.69 g/100 g dry weight (DW). A total of 41 compounds were identified in the EOs, with oxygenated monoterpenes (such as sabinyl acetate, cis-chrysanthenol, chrysantenyl acetate, 1,8-cineole, α- and β-thujone) as dominants, showing considerable variation among populations. Based on the EO profiles, several chemotypes were distinguished, mainly (1) a pure sabinyl acetate chemotype; (2) mixed chemotypes with sabinyl acetate accompanied by β-myrcene, cis-chrysanthenol, chrysanthenyl acetate, or 1,8-cineole; and (3) a thujone chemotype. The total content of phenolic acids (expressed as caffeic acid equivalent), tannins, and polyphenols (as pyrogallol equivalent) varied significantly, too (0.37–0.50; 0.10–0.26; 0.58–0.79%, respectively). The results confirm a high level of intraspecific variability in both developmental and chemical traits of A. absinthium populations originating from Poland. This diversity may be valuable for future breeding programs and for the selection of populations with desired phytochemical profiles for medicinal, food, and agricultural applications. It is worth noting that the floristic diversity among populations indicates the habitat heterogeneity, ranging from natural or semi-natural (populations 1, 6) to more anthropogenically influenced ones (populations 2, 4, 5, 7–11). Full article

Background and Objectives: Bell’s palsy, characterized by acute idiopathic facial nerve paralysis, exhibits variable recovery outcomes influenced by treatment timing, modality, and patient comorbidities. This study aimed to compare the effectiveness of corticosteroid-based treatment (Ear, Nose, and Throat [ENT]), nerve blocks/physical therapy (Pain Medicine), and acupuncture/herbal medicine (Traditional Korean Medicine [KM]) and identify predictors of recovery and recurrence. This retrospective cohort study leverages South Korea’s pluralistic healthcare system, where patients choose specialties, to provide novel insights into departmental treatment outcomes. Materials and Methods: We analyzed 600 patients treated within 72 h of Bell’s palsy onset (2010–2024) at Wonkwang University Hospital, South Korea, using propensity score matching (PSM) (1:1:1) for age, sex, comorbidities, and initial House–Brackmann (HB) grade. The primary outcome was complete recovery (HB grade I) at 6 months; secondary outcomes included recovery time, recurrence, complications, and patient satisfaction. Multivariate logistic regression identified predictors. Results: The ENT group achieved the highest complete recovery rate (87.5%, phi = 0.18) versus Pain Medicine (74.0%) and KM (69.5%) (p < 0.001), with the shortest recovery time (4 weeks, Cohen’s d = 0.65 vs. KM). Synkinesis was lowest in the ENT group (6.0%). ENT treatment (OR: 1.75; 95% CI: 1.29–2.37) and early corticosteroid application (OR: 1.95; 95% CI: 1.42–2.68) predicted recovery. Hypertension (OR: 4.40), hyperlipidemia (OR: 8.20), and diabetes (OR: 1.40) increased recurrence risk. Subgroup analyses showed that ENT treatment was most effective for severe cases (HB grade IV: 90% recovery vs. 65% in KM, p < 0.01). Conclusions: Corticosteroid-based treatment (ENT) yielded superior recovery outcomes. Comorbidity management is critical for recurrence prevention. Early ENT referral and integrated care models are recommended to optimize outcomes in diverse healthcare settings. Full article

Background and Objectives: The spontaneous reporting (SR) of adverse drug events (ADEs) is a cornerstone of pharmacovigilance and a critical mechanism for safeguarding patient safety. However, underreporting remains a persistent global challenge. In Korea, despite the widespread use of herbal medicines (HMs), adverse event reports from traditional Korean medicine doctors (KMDs) are remarkably scarce. This study aimed to assess the knowledge, attitudes, and experiences of KMDs regarding SR, identify key barriers, and suggest strategies to strengthen the safety culture within traditional Korean medicine. Materials and Methods: A cross-sectional, anonymous online survey was distributed to licensed KMDs registered with the Association of Korean Medicine. The questionnaire collected information on respondents’ sociodemographic characteristics and assessed their knowledge, attitudes, and experiences related to ADE reporting. Descriptive statistics and multivariate logistic regression were used to analyze the associations between the variables. Results: Of the 1021 KMDs who completed the survey, the vast majority acknowledged the importance of SR and recognized their role in pharmacovigilance. Nevertheless, only 5% had ever submitted an ADE report. A widespread lack of awareness about the national spontaneous reporting system (SRS), particularly its inclusion of licensed HMs, was evident. Although many respondents expressed support for expanding the scope of SR to cover all HMs, significant gaps in pharmacovigilance knowledge and limited access to relevant training were major barriers. KMDs affiliated with academic institutions or specialist groups showed higher levels of awareness, education, and reporting behavior. Conclusions: While KMDs exhibit positive attitudes toward patient safety and understand the importance of SR, their participation in it remains low due to knowledge deficits and insufficient training. Addressing these gaps through targeted education and expanding the national SRS to comprehensively include herbal medicines are essential steps toward enhancing pharmacovigilance and cultivating a proactive safety culture in Korean medicine. Full article

White mulberry (Morus alba L.) is a tree growing up to 15 m in height. It is a plant whose cultivation is historically associated with silk production. Mulberry leaves are the only food source of the mulberry silkworm caterpillars (Bombyx mori L.). The cultivation of this tree has recently gained renewed importance. Due to the content of numerous bioactive substances, mulberry is a valuable raw material for the food, pharmaceutical and herbal industries. This article presents the results of tests on pellets from 1-, 3- and 5-year-old branches, which are waste biomass remaining after pruning mulberry shrubs cultivated to obtain leaves to feed silkworms. Additionally, analyses of pellets from mulberry leaves were also carried out. For the specified mulberry biomass yield, analyses of chemical composition of mulberry biomass (branches and leaves) were carried out, and energy properties (heat of combustion and calorific value) and energy potential were calculated. The heat of combustion of pellet from mulberry branches was, on average, 19,266 MJ∙Mg⁻¹, and the calorific value was 17,726 MJ∙Mg⁻¹. The energy potential, on the other hand, was, on average, 159 GJ∙ha⁻¹ and 44 MWh∙ha⁻¹. The obtained results indicate the possibility of the effective use of mulberry branches after the annual pruning of bushes in plantations for energy purposes. Full article

Poplar leaves (Populi folium) are a herbal remedy traditionally used for the treatment of rheumatic diseases and prostate inflammation. The aim of our study was a comprehensive identification of secondary metabolites occurring in the leaves of Populus alba, Populus × candicans, and Populus nigra, in order to search for a source of raw plant material rich in active compounds. Total salicylate (TSC), flavonoid (TFC), and phenolic compound (TPC) contents were determined, and the antioxidant potential was assessed using DPPH (2,2-diphenyl-1-picrylhydrazyl), ABTS (2,2′-azino-bis(3-ethylbenzothiazoline- 6-sulfonic acid) diammonium salt), and FRAP (ferric reducing antioxidant power) assays as well as 2D-TLC (two-dimensional thin layer chromatography) bioautography using DPPH, riboflavin-light-NBT (nitro blue tetrazolium chloride), and xanthine oxidase inhibition tests. Secondary metabolites present in the analyzed poplar leaves were identified under the developed HPLC-DAD-ESI/MS (high performance liquid chromatography with photodiode array detection and electrospray ionization mass spectrometric detection analysis conditions and using the 2D-TLC method. Among the 80 identified compounds, 13 were shown for the first time in the genus Populus. The most diverse and similar set of flavonoids characterized the leaves of P. × candicans and P. nigra, while numerous salicylic compounds were present in the leaves of P. alba and P. × candicans. All analyzed leaves are a rich source of phenolic compounds. The highest flavonoid content was found in the leaves of P. × candicans and P. nigra, while the leaves of P. alba were characterized by the highest content of salicylates. All examined poplar leaves demonstrated antioxidant potential in all the assays used, which decreased in the following order: P. nigra, P. × candicans, P. alba. Full article

Zinc oxide nanoparticles (ZnONPs) are increasingly used in agriculture to stimulate plant growth and development, including under in vitro culture conditions. However, there is limited data on the effects of ZnONPs on the micropropagation of Scutellaria baicalensis Georgi. The pharmacological properties of this species make it a valuable medicinal plant. In Poland, it does not occur naturally but is cultivated for the production of herbal material. In vitro micropropagation is an effective method for obtaining genetically uniform plantlets. The aim of this study was to evaluate the effects of various concentrations of ZnONPs on growth parameters and the content of mineral nutrients, phenolic compounds, antioxidants, and photosynthetic pigments in Scutellaria baicalensis cultured in vitro. Shoot tip explants were cultured on MS medium supplemented with 1.0 mg dm⁻³ BA and 0.1 mg dm⁻³ IBA, together with ZnONPs at concentrations of 0 (control), 10, 20, 30, and 40 mg dm⁻³. The results showed that ZnONPs at concentrations of 10–20 mg dm⁻³ had no statistically significant effect on shoot or root development or on fresh weight gain. However, higher concentrations (30 and 40 mg dm⁻³) had a significantly negative impact on the number and length of shoots and roots, as well as on biomass accumulation. ZnONPs at 10–20 mg dm⁻³ significantly increased the content of potassium, calcium, magnesium, iron, and zinc in regenerated multi-shoot plantlets. A strong positive correlation (r = 0.951) was observed between ZnONP concentration and zinc accumulation in the plantlets. The levels of manganese and copper were not significantly different from the control. Plantlets treated with 30–40 mg dm⁻³ ZnONPs had significantly lower levels of calcium, iron, manganese, and copper. Those grown at 30 mg dm⁻³ had the highest potassium and magnesium levels, while plantlets exposed to 40 mg dm⁻³ had the highest zinc content. The total phenolic content and antioxidant activity (measured using ABTS and DPPH assays) were significantly higher in ZnONP-treated plantlets compared to the control. In contrast, the levels of chlorophyll a, chlorophyll b, total chlorophyll (a + b), and carotenoids were significantly lower in plants treated with ZnONPs. A strong negative correlation was found between ZnONP concentration and photosynthetic pigment content, while the ZnONP concentration was positively correlated with total phenolic content and antioxidant activity (ABTS⁺ and DPPH). Full article

The Quality by Design (QbD) concept has been widely applied to the optimization of traditional Chinese medicine production processes recently. This work focused on optimizing the critical purification process of Scutellaria baicalensis extract used in the preparation of Zhusheyong Shuanghuanglian. Considering the impact of noise parameters and changes in herbal properties, an experimental design method was employed for optimization. Multiple batches of Scutellaria baicalensis decoction were prepared in this research, and quantitative models of Scutellaria baicalensis herbal properties, critical process parameters (CPPs), and process evaluation indicators were established. The R² of the quantitative models were all higher than 0.80. According to the model, the yield of baicalin was identified as a critical material property (CMA). The pH of first acid precipitation (X₁), first temperature holding time (X₂), pH of alkalization (X₃), ethanol amount (X₄), and end pH of ethanol washing (X₅) were CPPs. Considering the difficulty in controlling the end pH of the ethanol washing, it was considered to be a noise parameter. The Monte Carlo probability-based method was used to calculate the design space, determining the range of controllable parameters, which was successfully validated through experiments. Normal operation ranges for controllable parameters are recommended as follows: X₁ of 0.8–2.2, X₂ of 25–35 min, X₃ of 6.5–7.5, and X₄ of 0.8–1.2 g/g. Full article

Chemotaxonomic profiling based on secondary metabolites offers a reliable approach for identifying and authenticating medicinal plants, addressing limitations associated with traditional morphological and genetic methods. Recent advances in microfluidics and nanoengineered technologies—including lab-on-a-chip systems as well as nano-enabled optical and electrochemical sensors—enable the rapid, accurate, and portable detection of key metabolites, such as alkaloids, flavonoids, terpenoids, and phenolics. Integrating artificial intelligence and machine learning techniques further enhances the analytical capabilities of these technologies, enabling automated, precise plant identification in field-based applications. Therefore, this review aims to highlight the potential applications of micro- and nanoengineered devices in herbal medicine markets, medicinal plant authentication, and biodiversity conservation. We discuss strategies to address current challenges, such as biocompatibility and material toxicity, technical limitations in device miniaturization, and regulatory and standardization requirements. Furthermore, we outline future trends and innovations necessary to fully realize the transformative potential of these technologies in real-world chemotaxonomic applications. Full article

The biological role of the fluorescence of flowers is a matter of debate. Anthocyanins are a group of compounds that are weakly fluorescent; their fluorescence in flowers has been rarely studied. This study aimed to compare the absorption and fluorescence spectra of anthocyanins extracted from several anthocyanin-containing autumn flowers and examine changes in these spectra during the storage of petals at cold-room and room temperatures and during the storage of dried petals. Petals of red clover Trifolium pratense, pink petunia Petunia × hybrida, Pelargonium horatum, Pelargonium. zonale, Pelargonium. peltatum, red and pink Begonia semperflorens, Buddleja japonica, and purple Chrysanthemum were studied. The results demonstrate that it is possible to distinguish between petals of various flowers based on the absorption spectra of petal extracts and the fluorescence spectra of petal extracts and intact petals. Spectral changes during storage were not always unidirectional and progressive; the most common one was the increase in the intensity of the fluorescence band at 500–560 nm at the excitation wavelength of 460 nm. These results point to the possibility of using fluorescence measurements to identify and estimate the freshness of petal-based material in herbalism, forensic analysis, and the food industry. The measurement of the spectra of whole petals or their fragments by front-face fluorimetry, including common plate readers, may be especially useful due to its simplicity and rapidity. Full article

The relationships between mycotoxins content in food commodities or feedstuffs and the foodborne diseases is well known. So far, the available data mainly include chemical methods of mycotoxins decontamination for agricultural commodities or raw materials, including mycotoxin binders. Therefore, the possible use of some natural and cost-effective supplements such as herbs, fungi, microorganisms, or plants with powerful and safe protection against mycotoxin-induced health ailments is the main subject of this review paper. Various antagonistic microorganisms or yeast with fungicidal properties, as well as some herbs or plants that suppress fungal development and the subsequent production of target mycotoxins and/or have protective effect against mycotoxins, are deeply studied in the literature, and practical suggestions are given in this regard. The protection by degradation, biotransformation, or binding of mycotoxins by using natural additives such as herbs or plants to feedstuffs or foods has also been thoroughly investigated and analyzed as a possible approach for ameliorating the target adverse effects of mycotoxins. Possible beneficial dietary changes have also been studied to potentially alleviate mycotoxin toxicity. Practical advice are provided for possible application of the same natural supplements in real-life practice for combating mycotoxin-induced health ailments. Natural feed supplements and bioactive compounds appeared to be safe emerging approaches to preventing health ailments caused by mycotoxins. However, the available data mainly address some in vitro studies, and more in vivo experiments are necessary for introducing such approaches in the real-life practice or industry. Generally, target herbal supplements, antioxidants, or polyenzyme complements could be used as powerful protectors in addition to natural mycotoxin binders. Bioactive agents and enzymatic degradation are reported to be very successful in regard to PAT and OTA, whereas antagonistic microorganisms/fungi/yeasts have a successful application against AFs and PAT-producing fungi. Full article