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Keywords = hardening spring

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21 pages, 1310 KB  
Article
Spring Warming Impact on the Reproductive and Vegetative Phenology and Biomass of Two Olive Cultivars in Argentina
by Leila M. Hamze, Peter S. Searles, Maria Agustina Iglesias and M. Cecilia Rousseaux
Plants 2026, 15(3), 493; https://doi.org/10.3390/plants15030493 - 5 Feb 2026
Viewed by 373
Abstract
Olive cultivation in warm regions is likely to be vulnerable to the expected temperature increases associated with climate change. The objectives of this study were to evaluate the effects of experimental warming at the end of late winter and spring on the timing [...] Read more.
Olive cultivation in warm regions is likely to be vulnerable to the expected temperature increases associated with climate change. The objectives of this study were to evaluate the effects of experimental warming at the end of late winter and spring on the timing of phenological stages, the duration of developmental periods, plant growth, and biomass production. The experiment was conducted in control (T0) and warmed (+4 °C, T+) open-top chambers (OTCs) during 2018 and 2019 using two olive cultivars (‘Arbequina’, ‘Coratina’) in northwest Argentina. Warming generally led to statistically significant earlier inflorescence development, flowering, fruit set, and pit hardening, with the responses being more pronounced as the spring progressed. Earlier vegetative bud opening occurred due to warming in 2018, but not in 2019. Additionally, no differences in shoot elongation or aboveground biomass were observed due to warming at the end of spring in either 2018 or 2019. Fruit set was reduced by warming, particularly in ‘Coratina’. Overall, the experimental results show that reproductive development is more sensitive to warming than vegetative growth in young olive trees. Further studies should be conducted in a larger number of olive cultivars and regions to improve our ability to predict responses to global warming. Full article
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20 pages, 1071 KB  
Article
Modeling for Data Efficiency: System Identification as a Precursor to Reinforcement Learning for Nonlinear Systems
by Nusrat Farheen, Golam Gause Jaman and Marco P. Schoen
Machines 2026, 14(2), 157; https://doi.org/10.3390/machines14020157 - 30 Jan 2026
Viewed by 484
Abstract
Safe and sample-conscious controller synthesis for nonlinear dynamics benefits from reinforcement learning that exploits an explicit plant model. A nonlinear mass–spring–damper with hardening effects and hard stops is studied, and off-plant Q-learning is enabled using two data-driven surrogates: (i) a piecewise linear model [...] Read more.
Safe and sample-conscious controller synthesis for nonlinear dynamics benefits from reinforcement learning that exploits an explicit plant model. A nonlinear mass–spring–damper with hardening effects and hard stops is studied, and off-plant Q-learning is enabled using two data-driven surrogates: (i) a piecewise linear model assembled from operating region transfer function estimates and blended by triangular memberships and (ii) a global nonlinear autoregressive model with exogenous input constructed from past inputs and outputs. In unit step reference tracking on the true plant, the piecewise linear route yields lower error and reduced steady-state bias (MAE = 0.03; SSE = 3%) compared with the NLARX route (MAE = 0.31; SSE = 30%) in the reported configuration. The improved regulation is obtained at a higher identification cost (60,000 samples versus 12,000 samples), reflecting a fidelity–knowledge–data trade-off between localized linearization and global nonlinear regression. All reported performance metrics correspond to deterministic validation runs using fixed surrogate models and trained policies and are intended to support methodological comparison rather than statistical performance characterization. These results indicate that model-based Q-learning with identified surrogates enables off-plant policy training while containing experimental risk and that performance depends on modeling choices, state discretization, and reward shaping. Full article
(This article belongs to the Special Issue Advances in Dynamics and Vibration Control in Mechanical Engineering)
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27 pages, 5609 KB  
Article
Characteristics of Compressive Stress Wave Propagation Across a Nonlinear Viscoelastic Filled Rock Joint
by Zhifa Zhan, Xiaolin Huang, Jiahu Du, Yilin Sun and Jilin Wang
Appl. Sci. 2026, 16(1), 428; https://doi.org/10.3390/app16010428 - 30 Dec 2025
Viewed by 311
Abstract
Filled joints significantly influence the dynamic response of rock masses, exhibiting coupled nonlinear compression-hardening and viscous deformation. However, the combined effects of these mechanisms on wave propagation remain unclear. This study develops a theoretical model based on a nonlinear viscoelastic formulation, in which [...] Read more.
Filled joints significantly influence the dynamic response of rock masses, exhibiting coupled nonlinear compression-hardening and viscous deformation. However, the combined effects of these mechanisms on wave propagation remain unclear. This study develops a theoretical model based on a nonlinear viscoelastic formulation, in which a compression-hardening spring (governed by the Bandis–Barton model, with its initial compressive stiffness and maximum allowable closure) is connected in series with a viscous dashpot. Using the displacement discontinuity method and the method of characteristics, we analyze the transmission of compressive stress waves across a filled joint. The results show that the transmission coefficient increases with incident wave amplitude but decreases with frequency, whereas reflection exhibits the opposite trends. The initial compressive stiffness has a minimal impact on transmission but induces a nonlinear decrease in reflection. Increasing the maximum allowable closure slightly reduces transmission but sharply increases reflection, whereas higher viscous stiffness enhances transmission and slightly suppresses reflection. Energy attenuation grows rapidly with amplitude before stabilizing. The initial compressive stiffness is most influential at low amplitudes, the maximum allowable closure is most significant at moderate amplitudes, and viscous effects remain consistent across all amplitudes. Increases in frequency lead to a nonlinear decrease in attenuation, with the initial compressive stiffness and maximum allowable closure dominating at high frequencies, and viscous effects prevailing at low frequencies. This work systematically reveals the coupled roles of nonlinear compression-hardening and viscosity in wave propagation across filled joints, providing theoretical support for dynamic hazard mitigation and geophysical exploration. Full article
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31 pages, 34012 KB  
Article
Finite Element Parametric Study of Nailed Non-Cohesive Soil Slopes
by Sohaib Ali Tarmom, Mohd. Ahmed, Mahmoud H. Mohamed, Meshel Q. Alkahtani and Javed Mallick
Symmetry 2025, 17(12), 2125; https://doi.org/10.3390/sym17122125 - 10 Dec 2025
Viewed by 546
Abstract
Computational modeling offers a cost-effective approach to exploring complex geotechnical behavior. This study uses PLAXIS 2D finite element software to simulate nailed soil slopes under plane strain conditions, with models calibrated against laboratory-scale experiments involving a sand-filled Perspex box, steel nail reinforcements, and [...] Read more.
Computational modeling offers a cost-effective approach to exploring complex geotechnical behavior. This study uses PLAXIS 2D finite element software to simulate nailed soil slopes under plane strain conditions, with models calibrated against laboratory-scale experiments involving a sand-filled Perspex box, steel nail reinforcements, and a rigid foundation. The soil mass, structural elements, and reinforcements are modeled using fifteen-node triangular elements, five-node plate elements, and two-node elastic spring elements, respectively. In this paper, parametric studies evaluate the influence of slope angles, mesh density, domain dimensions, constitutive models, and reinforcement configurations. Both prototype-scale and 3D-approximated models are included to assess scale effects and spatial behavior. The results highlight the significant impact of model size and material behavior, particularly when using the Hardening Soil model and its small-strain extension. Reinforcement optimization, including nail length reduction strategies, demonstrates the potential for maintaining slope stability while improving material efficiency. Validation against experimental data confirms that the numerical models accurately capture deformation patterns and internal stress development across different construction and loading phases. This study observed that the Hardening Soil (small-strain) material model significantly improved slope performance by reducing settlements and better capturing stress behavior, especially for steep slopes. Optimized redistribution of nail lengths across the slope depth enhanced stability while reducing reinforcement usage, demonstrating a cost-effective alternative to uniform configurations. The findings offer practical guidance for optimizing nailed slope stabilization in sandy soils, supporting safer and more economical geotechnical design for real-world applications. Full article
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15 pages, 4474 KB  
Article
Spectroscopic Study of Electrolytic-Plasma Discharge During Hardening of 20GL Steel and Its Effect on Microstructure and Mechanical Properties
by Bauyrzhan Rakhadilov, Rinat Kurmangaliyev, Nurlat Kadyrbolat, Rinat Kussainov, Zarina Satbayeva, Almasbek Maulit and Yerzhan Shayakhmetov
Crystals 2025, 15(11), 976; https://doi.org/10.3390/cryst15110976 - 13 Nov 2025
Viewed by 653
Abstract
This study investigated the electrolytic-plasma hardening (EPH) of cast 20GL steel, used for railway spring beams. The main objective was to analyze the spectral characteristics of the cathodic discharge and establish correlations between the plasma parameters, processing regimes, and resulting surface properties. Optical [...] Read more.
This study investigated the electrolytic-plasma hardening (EPH) of cast 20GL steel, used for railway spring beams. The main objective was to analyze the spectral characteristics of the cathodic discharge and establish correlations between the plasma parameters, processing regimes, and resulting surface properties. Optical emission spectroscopy revealed that the plasma at 260 V exhibited a high-energy state with an electron density of ~5.3 × 1016 cm−3 and an electron temperature of 10,031 K. Using these parameters, the heat flux from the plasma to the steel surface was estimated at ~1.5 × 107 W/m2, confirming that the discharge provides sufficient energy for surface austenitization. Microstructural analysis demonstrated that the electrolyte flow rate, which determines the cooling rate, is the key parameter controlling phase transformations. At low flow rates, ferrite–pearlite and bainitic structures formed, while a fully martensitic structure and maximum hardness (1046 HV) were achieved at 10 L/min. Tribological tests confirmed the superior wear resistance of the martensitic layers, showing a friction coefficient of 0.454 and a wear volume 3.4 times lower than in the as-cast state. These findings verify that EPH offers an energy-efficient, low-cost method for improving the surface performance and service life of 20GL steel components in heavy-duty railway applications. Full article
(This article belongs to the Section Inorganic Crystalline Materials)
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34 pages, 16849 KB  
Article
A Deformational Analysis of a Titanium Alloy Supported by the Mathematical Modelling of the Sheet Metal Forming Process via Numerical Simulation
by David Koreček, Pavel Solfronk and Jiří Sobotka
Materials 2025, 18(7), 1598; https://doi.org/10.3390/ma18071598 - 1 Apr 2025
Cited by 1 | Viewed by 980
Abstract
The issue of the mathematical modelling of the deformation process nowadays affects an indispensable part of the industrial stamping production process. In view of this issue, this paper focuses on the research and analysis of the mechanical properties and stress–strain characteristics of the [...] Read more.
The issue of the mathematical modelling of the deformation process nowadays affects an indispensable part of the industrial stamping production process. In view of this issue, this paper focuses on the research and analysis of the mechanical properties and stress–strain characteristics of the titanium alloy AMS4900. The investigated material properties and characteristics serve as input parameters defining the yield criterion in the environment of sheet metal forming process numerical simulations. Through the quality of the input parameters and the choice of the material model, the accuracy of the resulting numerical simulations is quite fundamentally affected. For this reason, the influence of the selected material models and the relevant material parameters with respect to the real sheet metal forming process is compared and evaluated in this paper. Full article
(This article belongs to the Section Advanced Materials Characterization)
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22 pages, 3301 KB  
Article
Cyclic Elasto-Plastic Behaviour of 51CrV4 Steel for Railway Parabolic Leaf Spring Design
by Vítor M. G. Gomes, Rita Dantas, José A. F. O. Correia and Abílio M. P. de Jesus
Appl. Sci. 2025, 15(5), 2549; https://doi.org/10.3390/app15052549 - 27 Feb 2025
Cited by 1 | Viewed by 996
Abstract
Parabolic leaf springs are components typically found in suspensions of freight railway rolling stock. These components are produced in high-strength alloyed steel, DIN 51CrV4, to resist severe loading and environmental conditions. Despite the material’s good mechanical characteristics, the geometric notches and high surface [...] Read more.
Parabolic leaf springs are components typically found in suspensions of freight railway rolling stock. These components are produced in high-strength alloyed steel, DIN 51CrV4, to resist severe loading and environmental conditions. Despite the material’s good mechanical characteristics, the geometric notches and high surface roughness that features its leaves might raise local stress levels to values above the elastic limit, with cyclic elasto-plastic behaviour models being more appropriate. In this investigation, the parameters of the Chaboche model combining the kinematic and isotropic hardening models are determined using experimental data previously obtained in strain-controlled cyclic tests. Once the parameters of the cyclic hardening model are determined, they are validated using a finite element approach considering the Chaboche cyclic plasticity model. As a result, the material properties specified in this investigation can be used in the fatigue mechanical design of parabolic leaf springs made with 51CrV4 (local approaches to notches and at surface roughness level) or even in other components produced with the same steel. Full article
(This article belongs to the Section Mechanical Engineering)
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17 pages, 4164 KB  
Article
Sterol Profile in Leaves of Spring Oats (Avena sativa L.) Under Conditions of the Cryolithozone
by Vasiliy V. Nokhsorov, Valentina E. Sofronova, Igor V. Sleptsov, Svetlana V. Senik, Lidia V. Petrova and Klim A. Petrov
Int. J. Plant Biol. 2024, 15(4), 1304-1320; https://doi.org/10.3390/ijpb15040090 - 12 Dec 2024
Cited by 1 | Viewed by 1442
Abstract
Plant sterols (STs) are essential for the regulation of fluidity and permeability of cell membranes, which have a wide structural diversity. The dynamics of changes in sterol molecular species in leaves of a valuable cereal crop, spring oat (Avena sativa L.), as [...] Read more.
Plant sterols (STs) are essential for the regulation of fluidity and permeability of cell membranes, which have a wide structural diversity. The dynamics of changes in sterol molecular species in leaves of a valuable cereal crop, spring oat (Avena sativa L.), as a function of different sowing dates were studied. In particular, 11 molecular species of sterols (STs) and triterpenoids in A. sativa leaves were identified by GC-MS. Triterpenoids Ψ-taraxasterol, cyclolaudenol, and betulin were identified in A. sativa leaves for the first time, which may be related to adaptation to extreme climatic conditions of the cryolithozone. The dynamics of STs and triterpenoids changes were revealed during growth and development of the standard term and late summer sowing term during A. sativa hardening to low ambient temperatures. The ratio of β-sitosterol to campesterol was found to increase in response to low positive air temperatures, while the ratio of stigmasterol to β-sitosterol remained constant from mid-September to the end of October. Overall, leaves of standard-seeded A. sativa plants maintained higher levels of absolute STs and triterpenoids by 1.9-fold than leaves of late-seeded A. sativa plants. It is suggested that the ability of A. sativa plants to synthesize β-sitosterol and stigmasterol may be part of an evolutionary adaptation process to cope with wide temperature fluctuations and to maintain important membrane-bound metabolic processes. Full article
(This article belongs to the Section Plant Physiology)
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17 pages, 4507 KB  
Article
Simplified Gravity Load Collapse Dynamic Analysis of Old-Type Reinforced Concrete Frames
by Konstantinos G. Megalooikonomou
Constr. Mater. 2024, 4(4), 704-720; https://doi.org/10.3390/constrmater4040038 - 14 Nov 2024
Viewed by 3901
Abstract
The results of shaking table tests from previous studies on a one-story, two-bay reinforced concrete frame—exhibiting both shear and axial failures—were compared with nonlinear dynamic analyses using simplified models intended to evaluate the collapse potential of older reinforced concrete structures. To replicate the [...] Read more.
The results of shaking table tests from previous studies on a one-story, two-bay reinforced concrete frame—exhibiting both shear and axial failures—were compared with nonlinear dynamic analyses using simplified models intended to evaluate the collapse potential of older reinforced concrete structures. To replicate the nonlinear behavior of columns, whether shear-critical or primarily flexure-dominant, a one-component beam model was applied. This model features a linear elastic element connected in series to a rigid plastic, linearly hardening spring at each end, representing a concentrated plasticity component. To account for strength degradation through path-dependent plasticity, a negative slope model as degradation was implemented, linking points at both shear and axial failure. The shear failure points were determined through pushover analysis of shear-critical columns using Phaethon software. Although the simplified model provided a reasonable approximation of the overall frame response and lateral strength degradation, especially in terms of drift, its reduced computational demands led to some discrepancies between the calculated and measured shear forces and drifts during certain segments of the time-history response. Full article
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23 pages, 7505 KB  
Article
Dynamic Analysis and PD Control in a 12-Pole Active Magnetic Bearing System
by Yigen Ren and Wensai Ma
Mathematics 2024, 12(15), 2331; https://doi.org/10.3390/math12152331 - 25 Jul 2024
Cited by 13 | Viewed by 2004
Abstract
This paper conducts an in-depth study on the dynamic stability and complex vibration behavior of a 12-pole active magnetic bearing (AMB) system considering gravitational effects under a PD controller. Firstly, based on electromagnetic theory and Newton’s second law, a two-degree-of-freedom control equation of [...] Read more.
This paper conducts an in-depth study on the dynamic stability and complex vibration behavior of a 12-pole active magnetic bearing (AMB) system considering gravitational effects under a PD controller. Firstly, based on electromagnetic theory and Newton’s second law, a two-degree-of-freedom control equation of the system, including PD control terms and gravitational effects, is constructed. This equation involves not only parametric excitation, quadratic nonlinearity, and cubic nonlinearity but also a more pronounced coupling effect between the magnetic poles due to the presence of gravity. Secondly, using the multi-scale method, a four-dimensional averaged equation of the system in Cartesian and polar coordinates is derived. Finally, through numerical analysis, the system’s amplitude–frequency response, motion trajectory, the relationship between energy and amplitude, and global dynamic behaviors such as bifurcation and chaos are discussed in detail. The results show that the PD controller significantly affects the system’s spring hardening/softening characteristics, excitation, amplitude, energy, and stability. Specifically, increasing the proportional gain can quickly suppress the rotor’s motion, but it also increases the system’s instability. Adjusting the differential gain can transition the system from a chaotic state to a stable periodic motion. Full article
(This article belongs to the Special Issue Modeling, Simulation and Control of Dynamical Systems)
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14 pages, 10274 KB  
Article
Influence of Nickel on Microstructure and Mechanical Properties in Medium-Carbon Spring Steel
by Qian Yu, Yuliang Zhao and Feiyu Zhao
Materials 2024, 17(10), 2423; https://doi.org/10.3390/ma17102423 - 17 May 2024
Cited by 8 | Viewed by 2701
Abstract
The effects of adding nickel on the phase transition temperature, microstructure, and mechanical properties of medium-carbon spring steel have been investigated. The results show that adding nickel reduces the martensite start (Ms) temperature, improves hardenability, and refines the sub-microstructure of [...] Read more.
The effects of adding nickel on the phase transition temperature, microstructure, and mechanical properties of medium-carbon spring steel have been investigated. The results show that adding nickel reduces the martensite start (Ms) temperature, improves hardenability, and refines the sub-microstructure of the martensite, thereby improving yield stress. The yield strength of martensitic steel increases by approximately 100 MPa due to a synergistic combination of grain refinement strengthening and dislocation strengthening, with an increase in the nickel content from 0 wt.% to 1 wt.%. The cryogenic impact toughness of martensitic steel also improved with a higher nickel content due to packet and block refinement and an increase in the proportion of high-angle grain boundaries (HAGBs). Full article
(This article belongs to the Special Issue Enhancing In-Use Properties of Advanced Steels)
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17 pages, 13214 KB  
Article
Numerical Analysis of Flow-Induced Transverse Vibration of a Cylinder with Cubic Non-Linear Stiffness at High Reynolds Numbers
by Sreeja Sadasivan, Grzegorz Litak and Michał Jan Gęca
Energies 2024, 17(7), 1776; https://doi.org/10.3390/en17071776 - 8 Apr 2024
Cited by 4 | Viewed by 2523
Abstract
Numerical calculations were performed to study the vortex-induced vibration (VIV) of a circular cylinder, which was elastically supported by springs of linear and cubic terms. These simulations were conducted at high Reynolds numbers ranging from 4200 to 42,000. To simulate the cylinder’s motion [...] Read more.
Numerical calculations were performed to study the vortex-induced vibration (VIV) of a circular cylinder, which was elastically supported by springs of linear and cubic terms. These simulations were conducted at high Reynolds numbers ranging from 4200 to 42,000. To simulate the cylinder’s motion and the associated aerodynamic forces, Computational Fluid Dynamics were employed in conjunction with dynamic mesh capabilities. The numerical method was initially verified by testing it with various grid resolutions and time steps, and subsequently, it was validated using experimental data. The response of cubic nonlinearities was investigated using insights gained from a conventional linear vortex-induced vibration (VIV) system. This 2D study revealed that both the amplitude and frequency of vibrations are contingent on the flow velocity. The highest output was achieved within the frequency lock-in region, where internal resonance occurs. In the case of a hardening spring, the beating response was observed from the lower end of the initial branch to the upper end of the initial branch. The response displacement amplitude obtained for the linear spring case was 27 mm, whereas in the cubic nonlinear case, the value was 31.8 mm. More importantly, the results indicate that the inclusion of nonlinear springs can substantially extend the range of wind velocities in which significant energy extraction through vortex-induced vibration (VIV) is achievable. Full article
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34 pages, 26836 KB  
Article
Analysis of TRIP Steel HCT690 Deformation Behaviour for Prediction of the Deformation Process and Spring-Back of the Material via Numerical Simulation
by David Koreček, Pavel Solfronk and Jiří Sobotka
Materials 2024, 17(3), 535; https://doi.org/10.3390/ma17030535 - 23 Jan 2024
Cited by 3 | Viewed by 2110
Abstract
This paper deals with the analysis of TRIP steel HCT690 deformation behaviour. The mechanical properties and deformation characteristics of the tested material are determined using selected material tests and tests that consider the required stress states used to define the yield criterion boundary [...] Read more.
This paper deals with the analysis of TRIP steel HCT690 deformation behaviour. The mechanical properties and deformation characteristics of the tested material are determined using selected material tests and tests that consider the required stress states used to define the yield criterion boundary condition and subsequent deformation behaviour in the region of severe plastic deformation. The measured data are subsequently implemented in the numerical simulation of sheet metal forming, where they are used as input data for the computational process in the form of a selected material model defining the yield criterion boundary and, furthermore, the material hardening law during deformation of the material. The chosen numerical simulation process corresponds to the sheet metal forming process, including the subsequent spring-back of the material, when the force does not affect the material. Furthermore, the influence of the chosen computational model and selected process parameters on the deformation and spring-back process of the material is evaluated. In addition to that, at the end of the paper, the results from the numerical simulation are compared with experimentally produced sheet stamping. Full article
(This article belongs to the Section Materials Simulation and Design)
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17 pages, 5318 KB  
Article
Predictive Modeling of Spring-Back Behavior in V-Bending of SS400 Steel Sheets under Elevated Temperatures Using Combined Hardening Laws
by Quy-Huy Trieu, Gia-Hai Vuong and Duc-Toan Nguyen
Appl. Sci. 2023, 13(18), 10347; https://doi.org/10.3390/app131810347 - 15 Sep 2023
Cited by 7 | Viewed by 2998
Abstract
This research presents an innovative methodology for accurately predicting spring-back tendencies in V-bending of SS400 steel sheets under elevated temperatures. The study leverages extensive tensile test data to determine parameters for pure isotropic and kinematic hardening laws at varying temperatures, crucial inputs for [...] Read more.
This research presents an innovative methodology for accurately predicting spring-back tendencies in V-bending of SS400 steel sheets under elevated temperatures. The study leverages extensive tensile test data to determine parameters for pure isotropic and kinematic hardening laws at varying temperatures, crucial inputs for Finite Element Method (FEM) simulations. While using pure isotropic or kinematic hardening laws alone has limitations, especially at elevated temperatures, a hybrid approach is recommended for robust predictive models in ABAQUS 6.13 software. To address this challenge, a novel method is introduced, utilizing flow stress curve ratios between elevated and room temperatures as a function of equivalent strain to derive combined hardening law parameters. Rigorous comparison of simulation and experimental results confirms the model’s effectiveness in predicting spring-back in the V-bending of SS400 steel sheets, particularly under elevated temperatures. This innovative approach enhances understanding of material behavior at high temperatures and improves predictive capabilities for designing and optimizing complex V-bending processes. Full article
(This article belongs to the Section Mechanical Engineering)
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11 pages, 1475 KB  
Article
Viscoelastic Properties of Zona Pellucida of Oocytes Characterized by Transient Electrical Impedance Spectroscopy
by Danyil Azarkh, Yuan Cao, Julia Floehr and Uwe Schnakenberg
Biosensors 2023, 13(4), 442; https://doi.org/10.3390/bios13040442 - 30 Mar 2023
Cited by 8 | Viewed by 3687
Abstract
The success rate in vitro fertilization is significantly linked to the quality of the oocytes. The oocyte’s membrane is encapsulated by a shell of gelatinous extracellular matrix, called zona pellucida, which undergoes dynamic changes throughout the reproduction cycle. During the window of highest [...] Read more.
The success rate in vitro fertilization is significantly linked to the quality of the oocytes. The oocyte’s membrane is encapsulated by a shell of gelatinous extracellular matrix, called zona pellucida, which undergoes dynamic changes throughout the reproduction cycle. During the window of highest fertility, the zona pellucida exhibits a softening phase, while it remains rigid during oocyte maturation and again after fertilization. These variations in mechanical properties facilitate or inhibit sperm penetration. Since successful fertilization considerably depends on the state of the zona pellucida, monitoring of the hardening process of the zona pellucida is vital. In this study, we scrutinized two distinct genetic mouse models, namely, fetuin-B wild-type and fetuin-B/ovastacin double deficient with normal and super-soft zona pellucida, respectively. We evaluated the hardening with the help of a microfluidic aspiration-assisted electrical impedance spectroscopy system. An oocyte was trapped by a microhole connected to a microfluidic channel by applying suction pressure. Transient electrical impedance spectra were taken by microelectrodes surrounding the microhole. The time-depending recovery of zona pellucida deflections to equilibrium was used to calculate the Young’s modulus and, for the first time, absolute viscosity values. The values were obtained by fitting the curves with an equivalent mechanical circuit consisting of a network of dashpots and springs. The observer-independent electrical readout in combination with a fitting algorithm for the calculation of the viscoelastic properties demonstrates a step toward a more user-friendly and easy-to-use tool for the characterizing and better understanding of the rheological properties of oocytes. Full article
(This article belongs to the Special Issue Impedance-Spectroscopy-Based Biosensors)
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