Search Results (84)

Petal blight caused by Neopestalotiopsis clavispora infection in Rhododendron delavayi petals severely reduces their ornamental value; however, the metabolic and transcriptional responses of petals to this pathogen remain unclear. In this study, we integrated widely targeted metabolomic and transcriptomic analyses to systematically characterize the response mechanisms of R. delavayi petals at early (1 day post-infection), middle (2 days post-infection), and late (4 days post-infection) stages of N. clavispora infection. A total of 1251 metabolites were identified, and K-means clustering analysis revealed that the differentially accumulated metabolites (DAMs) in Subclass 1 were significantly enriched in flavonoid, phenylpropanoid, and glutathione metabolic pathways. Transcriptomic analysis identified 3744, 6986, and 5407 differentially expressed genes (DEGs) at early, middle and late stages, respectively. Weighted gene co-expression network analysis (WGCNA) indicated that the key hub genes at early, middle, and late stages were mainly involved in maintaining reactive oxygen species (ROS) homeostasis, plant–pathogen interaction signal transduction, and cell wall remodeling, respectively. Joint metabolomic and transcriptomic analysis showed that phenylpropanoid, flavonoid, and glutathione pathways were commonly and significantly enriched. In vitro antifungal assays demonstrated that 2000 mg/L phloretin significantly inhibited mycelial growth of N. clavispora and effectively alleviated petal infection. Collectively, R. delavayi petals exhibit stage-specific defense strategies against N. clavispora: in the early stage, by inducing H₂O₂ accumulation and ROS homeostasis; in the middle stage, by activating immune signaling pathways; and in the late stage, by enhancing cell wall remodeling and antioxidant capacity. This study provides a theoretical basis and a candidate compound for green control of petal blight. Full article

Background/Objectives: In phenylketonuria (PKU), high blood phenylalanine (Phe) levels during pregnancy negatively influence foetal organogenesis and growth, leading to maternal PKU syndrome. Pregnancies must be carefully planned in order to maintain blood Phe levels ≤ 360 µmol/L pre-conception and throughout pregnancy. Our aim was to study metabolic control in PKU pregnancies across Europe. Methods: Eleven centres managing PKU participated. Data on blood Phe levels (µmol/L), natural protein intake (g/day), protein substitute intake (g/day) and maternal weight (kg) during pregnancy were collected retrospectively from dietetic records between 2012 and 2018. Results: In total, 84 female patients with PKU, accounting for 102 pregnancies (mean age: 30.4 ± 4.8 years), participated. Of these, 7 had hyperphenylalaninemia (HPA), 26 had mild PKU, 55 had classical PKU and 14 were unclassified. Sapropterin was prescribed in two pregnancies. Only 27% (28/102) of pregnancies successfully achieved consistent blood Phe levels ≤ 360 µmol/L for at least 2 weeks pre-conception. During pregnancy, 88% of blood Phe levels were ≤360 µmol/L, with a mean Phe of 229 ± 65 µmol/L. The mean number of blood Phe samples was 60 (1.5 per week) per pregnancy. In pre-pregnancy, over a mean of 2.9 years, only 35% of blood Phe levels were ≤360 µmol/L and 61% were <600 µmol/L. Post-pregnancy, over a mean of 2.8 years, 43% of Phe levels were <600 µmol/L with mean Phe 462 ± 226 µmol/L and 724 ± 230 µmol/L, respectively. 25% (25/102) had no levels performed post-pregnancy (mean of 2.8 ± 1.6 years) compared to 7% (7/102) pre-pregnancy (mean of 2.9 ± 1.5 years). Mean prescribed Phe intake pre-/during/post-pregnancy was 810 ± 721 vs. 787 ± 552 vs. 1110 ± 722 mg/day. Natural protein intake was 17 ± 15 vs. 17 ± 11 vs. 23 ± 15 g/day. Protein equivalent from protein substitute intake was 57 ± 21 vs. 66 ± 16 vs. 50 ± 23 g/day and total protein remained stable, 73 ± 14 vs. 83 ± 14 vs. 71 ± 19 g/day (1.1 ± 0.3 vs. 1.1 ± 0.4 vs. 1.0 ± 0.4 g/kg/day). Conclusions: Although a high level of metabolic control was maintained during pregnancy, fewer than 30% of pregnancies achieved constant Phe levels ≤ 360 µmol/L prior to conception, with minimal monitoring post-pregnancy. The long-term impact on the offspring remains unknown and requires further investigation. Full article

Water balance calculations at the watershed scale are fundamental to water resource planning and the sustainable management of limited water supplies. These calculations rely on stream and canal gaging networks operated by local, state and federal entities, whose availability has varied over time due to cost, staffing constraints, and limitations on suitable gaging locations. The Green River Basin (GRB) above Fontenelle Dam in Wyoming illustrates this trend, where the number of operational stream gaging sites has varied over time and the majority of locations have less than 15 years of streamflow records. Recent advancements in the ability to perform streamflow reconstruction and estimate agricultural water use offer a new avenue for estimating the water balance for watersheds with discontinuous gage observations. But the use of these datasets and approaches has not been tested. Therefore, this paper proposes and tests a novel framework that combines discontinuous streamflow observations with new datasets (OpenET, ET-Demands, and GEOGLOWS) to calculate monthly water balances in the GRB from water year 1991 to 2023. Focusing on two main test basins, the Green River and the New Fork River, the integration of modern datasets enables the successful calculation of the water balance in the GRB with good agreement with downstream gaging records, achieving a Nash–Sutcliffe efficiency (NSE) of 0.88 for the New Fork River and 0.80 for the Green River. By improving the ability to quantify water balance components in data-limited basins, this framework supports more transparent water accounting and informed decision-making for sustainable water management, including irrigation planning, drought response, and long-term resource allocation in semi-arid river systems. Full article

This study investigates how land use changes upstream and the Kamal Khan Dam have reshaped patterns of water allocation and intensified hydropolitical tensions in the Helmand/Hirmand Transboundary River Basin (HTRB). Remote sensing and deep learning techniques were employed to analyze land use changes from 2012 to 2024. After radiometric and atmospheric corrections were applied to Landsat imagery, pixel-based classification was performed using a Deep Belief Network model. Additionally, hydrological changes were analyzed using Sentinel-2 data, with attention paid to the diversion of water flows toward the Godzareh Depression. The classification results revealed considerable expansion of agricultural land downstream of the Kajaki Dam, primarily at the expense of rangeland, forest, and barren land. Moreover, sentinel imagery confirmed that, following the commissioning of the Kamal Khan Dam in 2021, systematically diverted Helmand/Hirmand hydrosystem flows toward the Godzareh Depression. Also, this study applied the painted water framework to analyze the evolution of hydropolitical dynamics in the HTRB across two critical periods, 1954–1980 and 2010–2025. The analysis revealed a fundamental transformation from green water-dominated natural flow regimes to infrastructure-controlled systems characterized by concurrent increases in both yellow water (controlled but not immediately consumed) and red water (controlled and consumed). The Kamal Khan Dam’s operationalization represents a pivotal inflection point, dramatically expanding Afghanistan’s yellow water reserves. This dual expansion of controlled water categories, empirically documented through satellite imagery, confirms the emergence of negative hydrohegemony in the basin. Consequently, Iran’s position has transitioned from a historically stable painted water class to one characterized by critical dependency. Full article

River sand is an essential resource for the construction industry and is extensively used worldwide. However, its limited availability has led to supply shortages and rising costs. As a non-renewable resource, sand accumulates behind river dams, and its extraction using mechanical dredgers harms river ecosystems. Overexploitation has depleted riverbeds and caused significant environmental concerns. The construction sector faces challenges such as water and sand scarcity, along with the use of substandard sand. Growing awareness of the environmental damage caused by excessive sand use has prompted researchers to explore alternatives to sand in cement and concrete. One promising approach is sand-free plastering, which is being investigated as a sustainable alternative to traditional sand-based plaster. We analyzed the physical properties of sand-free plaster materials and the development of an automated robotic system for applying these materials in green buildings. By promoting sustainability, these innovations aim to address the challenges posed by sand scarcity while minimizing environmental impacts. Full article

Southwest China’s karst region has developed a dam- and reservoir-dense pattern in which cascaded hydropower on mainstem rivers coexists with small hydropower on tributaries, forming a foundation for the region’s low-carbon energy supply. Under China’s “dual-carbon” targets and a strengthening ecological civilization agenda, it is urgent to clarify the mechanisms driving habitat quality (HQ) change under compound disturbances from cascaded hydropower, urbanization, and related pressures—especially the nonlinear pathway through which engineering disturbance propagates to ecological responses via land-use restructuring. To address this need, we develop a Cascade disturbance–Land restructuring–Habitat response chain framework and integrate an InVEST–IntPLUS–OPGD modeling approach to capture HQ dynamics in the Wujiang River Basin (1980–2020), attribute the interactive effects of coupled natural–social drivers, and project ecological responses under alternative 2035 scenarios. Results show that: (1) The basin maintained a stable ecological matrix, with forest land and cropland consistently >82.5% and forest cover near 50%, while construction land increased by 972.15 km² and water bodies by 354.23 km² (2) Mean HQ stayed high and declined by only 1.42%, with high and medium–high HQ dominating (>65%). HQ degradation is concentrated in urban expansion areas and reservoir shorelines, whereas most mountainous/forested regions remain stable; and (3) HQ spatial differentiation is mainly shaped by the synergy between forest structure and NDVI, while nonlinear urbanization edge effects impose stronger stress than hydropower development itself. Scenario simulations further indicate that a water protection pathway can enhance HQ by building integrated “water–forest” corridors that promote blue–green synergy. Overall, this study supports improved trade-off design between energy supply and ecological protection in vulnerable karst regions. Full article

Molecular sex determination in Syrian hamsters (Mesocricetus auratus) has been limited by the incomplete annotation of Y-linked loci in currently available genome assemblies. Here, we evaluate the Y-linked gene PRSSLY, which encodes a testis-specific serine protease-like protein, as a molecular marker for genetic sexing of Syrian hamster embryonic and placental tissues. Primers flanking a conserved PRSSLY coding region produced a male-specific amplicon showing 100% concordance with results from the established KDM5C/KDM5D PCR assay in E15.5 tail biopsies. SYBR Green–based qPCR enables the accurate detection of PRSSLY, characterized by a unique melt-curve profile, exclusively in male samples, allowing for efficient and sensitive mid-throughput analysis. Application of the PRSSLY assay to 417 placental samples from 39 dams demonstrated its suitability for large-scale sex genotyping, enabling sex assignment in the majority of samples despite the intrinsic complexity of placental tissue containing both maternal and embryonic genetic material. This assay provides a robust and reproducible approach for accurate sex genotyping in developmental and reproductive studies using Syrian hamsters. Full article

Water reservoirs are critical components of hydrological systems that mitigate floods and droughts, but their long-term performance under climate change and variable socioeconomic conditions remain insufficiently documented. This study examines the Bahlui River basin (northeastern Romania), where 17 reservoirs constructed mainly between the 1960s and 1980s have been operational for more than five decades. Using the most recent technical reservoir reports, land-use evolution, and present operational functions, the contribution of man-made reservoirs to flood attenuation and drought buffering over time was appraised. Flood mitigation is the most consistent and reliable function, with peak-flow reductions commonly exceeding 60–90% of design discharges at the basin scale. Engineered drought mitigation functions (irrigation and industrial water supply) have decreased significantly as a result of socioeconomic changes started in 1989. However, the gradual expansion of green infrastructure, such as wetlands and riparian vegetation, has improved passive water retention and low-flow buffering capacity. These unanticipated developments have resulted in variable levels of hybrid hydrological resilience. The findings show that, while artificial reservoirs have strong flood-control capacity over long periods of time, their contribution to drought mitigation is increasingly dependent on the integration of ecological components, emphasizing the importance of green-gray interactions in long-term reservoir management. Full article

Leaf color mutants are valuable resources for studying photosynthesis, pigment metabolism, and gene regulatory networks in plants. In this study, a naturally occurring sweetpotato albino mutant exhibiting a stable white-leaf phenotype across developmental stages was identified and compared with its green-leaf wild type to elucidate the molecular mechanisms underlying albinism. The mutant showed a dramatic 98.8% reduction in total chlorophyll content and a markedly decreased Fv/Fm value (0.59), indicating severe impairment of PSII efficiency. Integrated transcriptomic analysis identified 3520 differentially expressed genes (DEGs), while metabolomic profiling revealed 270 differentially accumulated metabolites (DAMs). Genes involved in chlorophyll and carotenoid biosynthesis, chloroplast development, and photosynthetic electron transport were strongly repressed, including key regulators such as GLK1, PORA, and PORB. Metabolomic alterations were mainly enriched in flavonoids, phenylpropanoids, and amino acid-derived pathways, reflecting broad reprogramming of both primary and secondary metabolism. These changes were accompanied by severely disrupted chloroplast ultrastructure, suggesting a primary defect in plastid development. Collectively, the integrated multi-omics evidence provides a comprehensive understanding of the coordinated transcriptional and metabolic alterations driving the albino phenotype in sweetpotato and establishes this mutant as a potential model for studying the interplay between chloroplast biogenesis, photosynthesis, and secondary metabolism. Full article

Lycium ruthenicum Murr. is a highly nutritional cash crop due to its fruit-abundant anthocyanins. With the development of the fruit, the color changes from green to dark purple and the anthocyanin content gradually increases. But the molecular mechanism of the anthocyanin biosynthesis process in L. ruthenicum fruit is still unclear. Five stages of L. ruthenicum fruit based on the color of the pericarp and flesh (BS1–BS5) were used for metabolomics and transcriptomics analyses to investigate the underlying mechanisms of the pigmentation. At the BS3 stage, the anthocyanin content was significantly increased and reached the highest level at the BS5 stage. A total of 25 DAMs related to flavonoids were identified by metabolomics, presenting a gradual increase with fruit development. Delphinidin-3-O-rutinoside and petunidin-3-O-rutinoside were identified as the main anthocyanins. Transcriptome sequencing and DEG analysis identified the key structural genes and transcription factors related to anthocyanin biosynthesis. Anthocyanin accumulation was driven mainly by the upregulation of six structural genes (F3′5′H, DFR, ANS, and UFGT) and eight key transcription factors from the HB, NAC, WRKY, Tify, AP2/ERF, and bHLH families that were significantly correlated with anthocyanin content in L. ruthenicum fruit. This study reveals key candidate genes in the anthocyanin biosynthetic pathway, providing new insights for improving fruit quality. Full article

The production of concrete strongly influences the environment. It is a versatile and sustainable construction material capable of creating a wide range of structures. It has always been indispensable as a material for the engineering and construction industry, including applications in hydraulic structures (e.g., dams, underwater tunnels, sluices, and other concrete structures), where mass concrete is a fundamental material in the construction industry. Developing sustainable concrete as an alternative construction material to the traditional one provides a reduction in the carbon dioxide footprint with regard to cement use and waste material disposal in landfills. This paper provides a comprehensive review of current trends and opportunities in sustainable construction using mass concrete. It underscores the importance of incorporating eco-friendly practices to mitigate environmental impact by using by-products as green materials. The review highlights how optimizing clinker content, supplementary cementitious materials (SCMs), and aggregates can improve the strength, durability, and thermal stability of mass concrete. Strategic material selection helps minimize thermal cracking, extend service life, and reduce environmental impact. Future research should focus on developing advanced mix design strategies and standardized practices for sustainable infrastructure. Full article

Background: Fruit skin color is a key determinant of grape quality and market value, primarily governed by anthocyanin biosynthesis. Methods: In this study, we explored the molecular basis of skin color variation in the grape cultivar Zaoheibao and its bud mutant, which displays a striking shift from purple-black to yellow-green. Integrated metabolomic and transcriptomic analyses revealed extensive reprogramming of the flavonoid pathway in the mutant. Results: Metabolite profiling identified 233 differentially accumulated metabolites (DAMs), with a drastic reduction in anthocyanins, particularly cyanidin- and peonidin-derivatives, together with altered levels of flavonols and flavonoid glycosides. Transcriptome analysis detected 4036 differentially expressed genes (DEGs), with key anthocyanin biosynthetic genes (DFR, ANS, UFGT, GST) and MYB significantly downregulated. Multi-omics integration confirmed consistent enrichment of flavonoid-related pathways, while correlation network analysis highlighted strong associations between MYB regulators, structural genes, and anthocyanin-type metabolites. Conclusions: Collectively, these findings demonstrate that suppression of a MYB-centered regulatory module underlies the loss of pigmentation in the bud mutant, providing new insights into the molecular regulation of grape skin coloration and a theoretical basis for grape breeding and quality improvement. Full article

With the characteristic of “decentralized distribution and local power supply”, small hydropower (SHP) in China has become a core means of solving the problem of insufficient power supply in rural and remote mountainous areas, effectively promoting the improvement of local livelihoods. However, for a long time, SHP has had many problems, such as irrational development, old equipment, and poor economic efficiency, resulting in some rivers with connectivity loss and reduced biodiversity, etc. The Chishui River Watershed is an ecologically valuable river in the upper reaches of the Yangtze River. As an important habitat for rare fish in the upper reaches of the Yangtze River and the only large-scale tributary that maintains a natural flow pattern, the SHP plants’ dismantling and ecological restoration practices in the Chishui River Watershed can set a model for regional sustainable development. This paper adopts the methods of literature review, field research, and case study analysis, combined with the comparison of ecological conditions before and after the dismantling, to systematically analyze the effectiveness and challenges of SHP rectification in the Chishui River Watershed. The study found that after dismantling 88.2% of SHP plants in ecologically sensitive areas, the number of fish species upstream and downstream of the original dam site increased by about 6.67% and 70%, respectively; the natural hydrological connectivity has been restored to the downstream of the Tongzi River, the Gulin River and other rivers, but there are short-term problems such as sediment underflow, increased economic pressure, and the gap of alternative energy sources; the retained power stations have achieved the success and challenges of power generation and ecological management ecological flow control and comprehensive utilization, achieving a balance between power generation and ecological protection. Based on the above findings, the author proposes dynamic monitoring and interdisciplinary tracking research to fill the gap of systematic data support and long-term effect research in the SHP exit mechanism, and the results can provide a reference for the green transition of SHP. Full article

This study examines research trends and identifies key gaps relevant to the field of structural safety and resilience; additionally, a systematic literature review (SLR) guided by the PRISMA methodology was conducted, analyzing 4188 documents ranging from 1975 to 2025. The research revealed key trends, including a focus on various aspects of the structural stability and resilience of buildings affected by earthquakes through analysis of various innovative methods and materials. The present study encompasses work describing the use of steel–wood composite columns to improve building stability, assessment of the impact of wood accumulation on bridges during floods, and the effect of debris flow on the stability of check dams. In addition, this study also evaluates the seismic performance of school buildings in Mexico, a method of diagnosing cracks in concrete dams, and the application of recycled materials from old tires for seismic disaster mitigation. Acoustic emission monitoring methods in medieval towers and the design of seismic isolation systems with variable damping are also discussed. Bibliometric analysis highlighted increased collaboration and a thematic shift towards green and data-driven approaches. However, significant gaps were identified. The findings explain that the use of innovative materials and methods can improve the stability and resistance of building structures with respect to dynamic loads, such as those associated with earthquakes and floods. The findings provide guidance for the design and maintenance of safer and more sustainable infrastructure in the future. Full article

Sulfur fumigation (SF), acid dipping (HCl treatment, HAT), and their combination (SF+HAT) are common methods for long-term preservation and color protection of litchi. However, their effects on the metabolic profile of the litchi pericarp have not been investigated. SF resulted in a yellowish-green pericarp by up-regulating lightness (L*), b*, C*, and h° but down-regulating total anthocyanin content (TAC) and a*, while HAT resulted in a reddish coloration by up-regulating a*, b*, and C* but down-regulating L*, h°, and TAC. SF+HAT recovered reddish color with similar L*, C* to SF but a*, b*, h°, and TAC between SF and HAT. Differential accumulated metabolites (DAMs) detected in HAT (vs. control) were more than those in SF (vs. control), but similar to those in SF+HAT (vs. control). SF specifically down-regulated the content of cyanidin-3-O-rutinoside, sinapinaldehyde, salicylic acid, and tyrosol, but up-regulated 6 flavonoids (luteolin, kaempferol-3-O-(6″-malonyl)galactoside, hesperetin-7-O-glucoside, etc.). Five pathways (biosynthesis of phenylpropanoids, flavonoid biosynthesis, biosynthesis of secondary metabolites, glutathione metabolism, and cysteine and methionine metabolism) were commonly enriched among the three treatments, which significantly up-regulated sulfur-containing metabolites (mainly glutathione, methionine, and homocystine) and down-regulated substrates for browning (mainly procyanidin B2, C1, and coniferyl alcohol). These results provide metabolic evidence for the effect of three treatments on coloration and storability of litchi. Full article