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11 pages, 638 KiB  
Communication
Millet in Bioregenerative Life Support Systems: Hypergravity Resilience and Predictive Yield Models
by Tatiana S. Aniskina, Arkady N. Kudritsky, Olga A. Shchuklina, Nikita E. Andreev and Ekaterina N. Baranova
Life 2025, 15(8), 1261; https://doi.org/10.3390/life15081261 (registering DOI) - 7 Aug 2025
Abstract
The prospects for long-distance space flights are becoming increasingly realistic, and one of the key factors for their implementation is the creation of sustainable systems for producing food on site. Therefore, the aim of our work is to assess the prospects for using [...] Read more.
The prospects for long-distance space flights are becoming increasingly realistic, and one of the key factors for their implementation is the creation of sustainable systems for producing food on site. Therefore, the aim of our work is to assess the prospects for using millet in biological life support systems and to create predictive models of yield components for automating plant cultivation control. The study found that stress from hypergravity (800 g, 1200 g, 2000 g, and 3000 g) in the early stages of millet germination does not affect seedlings or yield. In a closed system, millet yield reached 0.31 kg/m2, the weight of 1000 seeds was 8.61 g, and the yield index was 0.06. The paper describes 40 quantitative traits, including six leaf and trichome traits and nine grain traits from the lower, middle and upper parts of the inflorescence. The compiled predictive regression equations allow predicting the accumulation of biomass in seedlings on the 10th and 20th days of cultivation, as well as the weight of 1000 seeds, the number of productive inflorescences, the total above-ground mass, and the number and weight of grains per plant. These equations open up opportunities for the development of computer vision and high-speed plant phenotyping programs that will allow automatic correction of the plant cultivation process and modeling of the required yield. Predicting biomass yield will also be useful in assessing the load on the waste-free processing system for plant waste at planetary stations. Full article
(This article belongs to the Special Issue Physiological Responses of Plants Under Abiotic Stresses)
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17 pages, 2019 KiB  
Article
Sport Participation and Gender Differences in Dietary Preferences: A Cross-Sectional Study in Italian Adults
by Francesca Campoli, Elvira Padua, Michele Panzarino, Lucio Caprioli, Giuseppe Annino and Mauro Lombardo
Sports 2025, 13(8), 258; https://doi.org/10.3390/sports13080258 - 6 Aug 2025
Abstract
Background: The relationship between sports participation and food preferences in adults, as well as the influence of gender, is still unclear. Objective: The objective of this study was to investigate the association between sports participation and individual food preferences and to explore potential [...] Read more.
Background: The relationship between sports participation and food preferences in adults, as well as the influence of gender, is still unclear. Objective: The objective of this study was to investigate the association between sports participation and individual food preferences and to explore potential gender differences among sports participants in a large group of Italian adults. Methods: This cross-sectional study involved 2665 adults (aged ≥ 18 years) who lead normal lives and underwent a routine lifestyle and dietary assessment at a clinical centre specialising in nutrition, metabolic health, and lifestyle counselling in Rome. Participants completed an online questionnaire on food preferences (19 foods) and sports practice. Multivariate logistic regression models, adjusted for age, sex, and smoking, were used to assess associations. Results: Sports participation was defined as engaging in structured physical activity at least once per week and was reported by 53.5% of subjects (men: 60.1%; women: 49.0%; p < 0.0001). After adjustment, active individuals were significantly more likely to prefer plant-based drinks, low-fat yoghurt, fish, cooked and raw vegetables, fruit, whole grains, tofu, and dark chocolate (all p < 0.05) and less likely to prefer cow’s milk (p = 0.018). Among sport participants, males were more likely to prefer meat (general, white, red, processed) and eggs, while females preferred plant-based drinks. No significant gender differences were observed for dairy products, legumes, or fish. Differences in food preferences were also observed according to the type of sport, with bodybuilders showing higher preference for tofu and dark chocolate. The strongest associations were found in the 25–44 age group. Conclusions: Sports participation is independently associated with specific food preferences, characterised by greater preference for plant-based and fibre-rich foods, and gender differences in food choices persist even among active adults. These findings highlight the need to consider both sports participation and gender when designing nutritional interventions. Full article
(This article belongs to the Special Issue Enhancing Performance and Promoting Health Through Nutrition)
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12 pages, 2135 KiB  
Article
Development of Yellow Rust-Resistant and High-Yielding Bread Wheat (Triticum aestivum L.) Lines Using Marker-Assisted Backcrossing Strategies
by Bekhruz O. Ochilov, Khurshid S. Turakulov, Sodir K. Meliev, Fazliddin A. Melikuziev, Ilkham S. Aytenov, Sojida M. Murodova, Gavkhar O. Khalillaeva, Bakhodir Kh. Chinikulov, Laylo A. Azimova, Alisher M. Urinov, Ozod S. Turaev, Fakhriddin N. Kushanov, Ilkhom B. Salakhutdinov, Jinbiao Ma, Muhammad Awais and Tohir A. Bozorov
Int. J. Mol. Sci. 2025, 26(15), 7603; https://doi.org/10.3390/ijms26157603 - 6 Aug 2025
Abstract
The fungal pathogen Puccinia striiformis f. sp. tritici, which causes yellow rust disease, poses a significant economic threat to wheat production not only in Uzbekistan but also globally, leading to substantial reductions in grain yield. This study aimed to develop yellow rust-resistance [...] Read more.
The fungal pathogen Puccinia striiformis f. sp. tritici, which causes yellow rust disease, poses a significant economic threat to wheat production not only in Uzbekistan but also globally, leading to substantial reductions in grain yield. This study aimed to develop yellow rust-resistance wheat lines by introgressing Yr10 and Yr15 genes into high-yielding cultivar Grom using the marker-assisted backcrossing (MABC) method. Grom was crossed with donor genotypes Yr10/6*Avocet S and Yr15/6*Avocet S, resulting in the development of F1 generations. In the following years, the F1 hybrids were advanced to the BC2F1 and BC2F2 generations using the MABC approach. Foreground and background selection using microsatellite markers (Xpsp3000 and Barc008) were employed to identify homozygous Yr10- and Yr15-containing genotypes. The resulting BC2F2 lines, designated as Grom-Yr10 and Grom-Yr15, retained key agronomic traits of the recurrent parent cv. Grom, such as spike length (13.0–11.9 cm) and spike weight (3.23–2.92 g). Under artificial infection conditions, the selected lines showed complete resistance to yellow rust (infection type 0). The most promising BC2F2 plants were subsequently advanced to homozygous BC2F3 lines harboring the introgressed resistance genes through marker-assisted selection. This study demonstrates the effectiveness of integrating molecular marker-assisted selection with conventional breeding methods to enhance disease resistance while preserving high-yielding traits. The newly developed lines offer valuable material for future wheat improvement and contribute to sustainable agriculture and food security. Full article
(This article belongs to the Special Issue Molecular Advances in Understanding Plant-Microbe Interactions)
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17 pages, 1396 KiB  
Article
Dose-Dependent Effect of the Polyamine Spermine on Wheat Seed Germination, Mycelium Growth of Fusarium Seed-Borne Pathogens, and In Vivo Fusarium Root and Crown Rot Development
by Tsvetina Nikolova, Dessislava Todorova, Tzenko Vatchev, Zornitsa Stoyanova, Valya Lyubenova, Yordanka Taseva, Ivo Yanashkov and Iskren Sergiev
Agriculture 2025, 15(15), 1695; https://doi.org/10.3390/agriculture15151695 - 6 Aug 2025
Abstract
Wheat (Triticum aestivum L.) is a crucial global food crop. The intensive crop farming, monoculture cultivation, and impact of climate change affect the susceptibility of wheat cultivars to biotic stresses, mainly caused by soil fungal pathogens, especially those belonging to the genus [...] Read more.
Wheat (Triticum aestivum L.) is a crucial global food crop. The intensive crop farming, monoculture cultivation, and impact of climate change affect the susceptibility of wheat cultivars to biotic stresses, mainly caused by soil fungal pathogens, especially those belonging to the genus Fusarium. This situation threatens yield and grain quality through root and crown rot. While conventional chemical fungicides face resistance issues and environmental concerns, biological alternatives like seed priming with natural metabolites are gaining attention. Polyamines, including putrescine, spermidine, and spermine, are attractive priming agents influencing plant development and abiotic stress responses. Spermine in particular shows potential for in vitro antifungal activity against Fusarium. Optimising spermine concentration for seed priming is crucial to maximising protection against Fusarium infection while ensuring robust plant growth. In this research, we explored the potential of the polyamine spermine as a seed treatment to enhance wheat resilience, aiming to identify a sustainable alternative to synthetic fungicides. Our findings revealed that a six-hour seed soak in spermine solutions ranging from 0.5 to 5 mM did not delay germination or seedling growth. In fact, the 5 mM concentration significantly stimulated root weight and length. In complementary in vitro assays, we evaluated the antifungal activity of spermine (0.5–5 mM) against three Fusarium species. The results demonstrated complete inhibition of Fusarium culmorum growth at 5 mM spermine. A less significant effect on Fusarium graminearum and little to no impact on Fusarium oxysporum were found. The performed analysis revealed that the spermine had a fungistatic effect against the pathogen, retarding the mycelium growth of F. culmorum inoculated on the seed surface. A pot experiment with Bulgarian soft wheat cv. Sadovo-1 was carried out to estimate the effect of seed priming with spermine against infection with isolates of pathogenic fungus F. culmorum on plant growth and disease severity. Our results demonstrated that spermine resulted in a reduced distribution of F. culmorum and improved plant performance, as evidenced by the higher fresh weight and height of plants pre-treated with spermine. This research describes the efficacy of spermine seed priming as a novel strategy for managing Fusarium root and crown rot in wheat. Full article
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15 pages, 1507 KiB  
Article
Determination of Fumonisins B1 and B2 in Food Matrices: Optimisation of a Liquid Chromatographic Method with Fluorescence Detection
by Óscar Cebadero-Domínguez, Santiago Ruiz-Moyano, Alberto Martín and Elisabet Martín-Tornero
Toxins 2025, 17(8), 391; https://doi.org/10.3390/toxins17080391 - 5 Aug 2025
Abstract
Fumonisins, primarily produced by Fusarium spp. and Aspergillus section nigri, are common contaminants in maize, cereal grains, and other processed and derived products, representing a significant risk to food safety and public health. This study presents the development and optimisation of a [...] Read more.
Fumonisins, primarily produced by Fusarium spp. and Aspergillus section nigri, are common contaminants in maize, cereal grains, and other processed and derived products, representing a significant risk to food safety and public health. This study presents the development and optimisation of a high-performance liquid chromatography method with fluorescence detection (HPLC-FLD) for the quantification of fumonisin B1 (FB1) and B2 (FB2) in various food matrices. In contrast with conventional protocols employing potassium phosphate buffers as the mobile phase, the proposed method utilises formic acid, offering enhanced compatibility with liquid chromatography systems. An automated online precolumn derivatisation with o-phthaldialdehyde (OPA) was optimised through experimental design and response surface methodology, enabling baseline separation of FB1 and FB2 derivatives in less than 20 min. The method demonstrated high sensitivity, with limits of detection of 0.006 µg mL−1 for FB1 and 0.012 µg mL−1 for FB2, and excellent repeatability (intraday RSD values of 0.85% and 0.83%, respectively). Several solid-phase extraction (SPE) strategies were evaluated to enhance sample clean-up using a variety of food samples, including dried figs, raisins, dates, corn, cornmeal, wheat flour, and rice. FumoniStar Inmunoaffinity columns were the only clean-up method that provided optimal recoveries (70–120%) across all tested food matrices. However, the MultiSep™ 211 column yielded good recoveries for both fumonisins in dried figs and raisins. Additionally, the C18 cartridge achieved acceptable recoveries for both fumonisins in dried figs and wheat flour. Full article
(This article belongs to the Section Mycotoxins)
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16 pages, 2097 KiB  
Article
Dual Bioconversion Strategy: Synergistic Germination and Lactobacillus Fermentation Engineering for a γ-Aminobutyric Acid-Enriched Beverage from Brown Rice
by Di Yuan, Shan Zhang, Bin Hong, Shan Shan, Jingyi Zhang, Dixin Sha, Shiwei Gao, Qing Liu, Shuwen Lu and Chuanying Ren
Foods 2025, 14(15), 2733; https://doi.org/10.3390/foods14152733 - 5 Aug 2025
Viewed by 16
Abstract
Growing demand for plant-based nutraceuticals drives the need for innovative bioprocessing strategies. This study developed an integrated approach combining germination and Lactobacillus-mediated fermentation to produce a γ-aminobutyric acid (GABA)-enriched functional beverage from brown rice. Systematic screening identified an optimal rice cultivar for germination. [...] Read more.
Growing demand for plant-based nutraceuticals drives the need for innovative bioprocessing strategies. This study developed an integrated approach combining germination and Lactobacillus-mediated fermentation to produce a γ-aminobutyric acid (GABA)-enriched functional beverage from brown rice. Systematic screening identified an optimal rice cultivar for germination. Sequential enzymatic liquefaction and saccharification were optimized to generate a suitable hydrolysate. Screening of 13 probiotic strains revealed that a 10-strain Lactobacillus–Bifidobacterium consortium maximized GABA synthesis (12.2 mg/100 g). Fermentation parameters were optimized to 0.25% monosodium glutamate, 4% inoculum, 10 μmol/L pyridoxine hydrochloride, 37 °C, and 24 h. The resulting beverage achieved significantly elevated GABA concentrations while exhibiting low fat (0.2 g/100 g), reduced caloric content (233.6 kJ/100 g), and high viable probiotic counts (2 × 108 CFU/g). This strategy demonstrates significant potential for the scalable production of multifunctional, plant-based nutraceuticals with targeted bioactive components. Full article
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14 pages, 1567 KiB  
Article
Determining the Benzo[a]pyrene Degradation, Tolerance, and Adsorption Mechanisms of Kefir-Derived Bacterium Bacillus mojavensis TC-5
by Zhixian Duo, Haohao Li, Zeyu Wang, Zhiwei Zhang, Zhuonan Yang, Aofei Jin, Minwei Zhang, Rui Zhang and Yanan Qin
Foods 2025, 14(15), 2727; https://doi.org/10.3390/foods14152727 - 4 Aug 2025
Viewed by 109
Abstract
Microbial detoxification, as an environmentally friendly strategy, has been widely applied for benzo[a]pyrene (BaP) degradation. Within this approach, food-derived microbial strains offer unique advantages in safety, specificity, and sustainability for detoxifying food-borne BaP. In this study, we aimed to explore the potential of [...] Read more.
Microbial detoxification, as an environmentally friendly strategy, has been widely applied for benzo[a]pyrene (BaP) degradation. Within this approach, food-derived microbial strains offer unique advantages in safety, specificity, and sustainability for detoxifying food-borne BaP. In this study, we aimed to explore the potential of such strains in BaP degradation. Bacillus mojavensis TC-5, a strain that degrades BaP, was isolated from kefir grains. Surprisingly, 12 genes encoding dehydrogenases, synthases, and oxygenases, including betB, fabHB, qdoI, cdoA, and bioI, which are related to BaP degradation, were up-regulated by 2.01-fold to 4.52-fold in TC-5. Two potential degradation pathways were deduced. In pathway I, dioxygenase, betaine aldehyde dehydrogenase, and beta-ketoacyl-ACP synthase III FabHB act sequentially on BaP to form 4H-pyran-4-one,2,3-dihydro-3,5-dihydroxy-6-methyl via the phthalic acid pathway. In the presence of the cytochrome P450 enzyme, BaP progressively mediates ring cleavage via the anthracene pathway, eventually forming 3-methyl-5-propylnonane in pathway II. Notably, TC-5 achieved an impressive BaP removal efficiency of up to 63.94%, with a degradation efficiency of 32.89%. These results suggest that TC-5 has significant potential for application in addressing food-borne BaP contamination. Moreover, our findings expand the application possibilities of Xinjiang fermented milk products and add to the available green strategies for BaP degradation in food systems. Full article
(This article belongs to the Section Nutraceuticals, Functional Foods, and Novel Foods)
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21 pages, 1932 KiB  
Article
Exploring Agronomic Management Strategies to Improve Millet, Sorghum, Peanuts and Rice in Senegal Using the DSSAT Models
by Walter E. Baethgen, Adama Faye and Mbaye Diop
Agronomy 2025, 15(8), 1882; https://doi.org/10.3390/agronomy15081882 - 4 Aug 2025
Viewed by 165
Abstract
Achieving food security for a growing population under a changing climate is a key concern in Senegal, where agriculture employs 77% of the workforce with a majority of small farmers who rely on the production of crops for their subsistence and for income [...] Read more.
Achieving food security for a growing population under a changing climate is a key concern in Senegal, where agriculture employs 77% of the workforce with a majority of small farmers who rely on the production of crops for their subsistence and for income generation. Moreover, due to the underproductive soils and variable rainfall, Senegal depends on imports to fulfil 70% of its food requirements. In this research, we considered four crops that are crucial for Senegalese agriculture: millet, sorghum, peanuts and rice. We used crop simulation models to explore existing yield gaps and optimal agronomic practices. Improving the N fertilizer management in sorghum and millet resulted in 40–100% increases in grain yields. Improved N symbiotic fixation in peanuts resulted in yield increases of 20–100% with highest impact in wetter locations. Optimizing irrigation management and N fertilizer use resulted in 20–40% gains. The best N fertilizer strategy for sorghum and millet included applying low rates at sowing and in early development stages and adjusting a third application, considering the expected rainfall. Peanut yields of the variety 73-33 were higher than Fleur-11 in all locations, and irrigation showed no clear economic advantage. The best N fertilizer management for rainfed rice included applying 30 kg N/ha at sowing, 25 days after sowing (DAS) and 45 DAS. The best combination of sowing dates for a possible double rice crop depended on irrigation costs, with a first crop planted in January or March and a second crop planted in July. Our work confirmed results obtained in field research experiments and identified management practices for increasing productivity and reducing yield variability. Those crop management practices can be implemented in pilot experiments to further validate the results and to disseminate best management practices for farmers in Senegal. Full article
(This article belongs to the Section Farming Sustainability)
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28 pages, 3364 KiB  
Review
Principles, Applications, and Future Evolution of Agricultural Nondestructive Testing Based on Microwaves
by Ran Tao, Leijun Xu, Xue Bai and Jianfeng Chen
Sensors 2025, 25(15), 4783; https://doi.org/10.3390/s25154783 - 3 Aug 2025
Viewed by 170
Abstract
Agricultural nondestructive testing technology is pivotal in safeguarding food quality assurance, safety monitoring, and supply chain transparency. While conventional optical methods such as near-infrared spectroscopy and hyperspectral imaging demonstrate proficiency in surface composition analysis, their constrained penetration depth and environmental sensitivity limit effectiveness [...] Read more.
Agricultural nondestructive testing technology is pivotal in safeguarding food quality assurance, safety monitoring, and supply chain transparency. While conventional optical methods such as near-infrared spectroscopy and hyperspectral imaging demonstrate proficiency in surface composition analysis, their constrained penetration depth and environmental sensitivity limit effectiveness in dynamic agricultural inspections. This review highlights the transformative potential of microwave technologies, systematically examining their operational principles, current implementations, and developmental trajectories for agricultural quality control. Microwave technology leverages dielectric response mechanisms to overcome traditional limitations, such as low-frequency penetration for grain silo moisture testing and high-frequency multi-parameter analysis, enabling simultaneous detection of moisture gradients, density variations, and foreign contaminants. Established applications span moisture quantification in cereal grains, oilseed crops, and plant tissues, while emerging implementations address storage condition monitoring, mycotoxin detection, and adulteration screening. The high-frequency branch of the microwave–millimeter wave systems enhances analytical precision through molecular resonance effects and sub-millimeter spatial resolution, achieving trace-level contaminant identification. Current challenges focus on three areas: excessive absorption of low-frequency microwaves by high-moisture agricultural products, significant path loss of microwave high-frequency signals in complex environments, and the lack of a standardized dielectric database. In the future, it is essential to develop low-cost, highly sensitive, and portable systems based on solid-state microelectronics and metamaterials, and to utilize IoT and 6G communications to enable dynamic monitoring. This review not only consolidates the state-of-the-art but also identifies future innovation pathways, providing a roadmap for scalable deployment of next-generation agricultural NDT systems. Full article
(This article belongs to the Section Smart Agriculture)
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28 pages, 2191 KiB  
Article
An Evaluation of Food Security and Grain Production Trends in the Arid Region of Northwest China (2000–2035)
by Yifeng Hao and Yaodong Zhou
Agriculture 2025, 15(15), 1672; https://doi.org/10.3390/agriculture15151672 - 2 Aug 2025
Viewed by 244
Abstract
Food security is crucial for social stability and economic development. Ensuring food security in the arid region of Northwest China presents unique challenges due to limited water and soil resources. This study addresses these challenges by integrating a comprehensive water and soil resource [...] Read more.
Food security is crucial for social stability and economic development. Ensuring food security in the arid region of Northwest China presents unique challenges due to limited water and soil resources. This study addresses these challenges by integrating a comprehensive water and soil resource matching assessment with grain production forecasting. Based on data from 2000 to 2020, this research projects the food security status to 2035 using the GM(1,1) model, incorporating a comprehensive index of soil and water resource matching and regression analysis to inform production forecasts. Key assumptions include continued historical trends in population growth, urbanization, and dietary shifts towards an increased animal protein consumption. The findings revealed a consistent upward trend in grain production from 2000 to 2020, with an average annual growth rate of 3.5%. Corn and wheat emerged as the dominant grain crops. Certain provinces demonstrated comparative advantages for specific crops like rice and wheat. The most significant finding is that despite the projected growth in the total grain output by 2035 compared to 2020, the regional grain self-sufficiency rate is projected to range from 79.6% to 84.1%, falling below critical food security benchmarks set by the FAO and China. This projected shortfall carries significant implications, underscoring a serious challenge to regional food security and highlighting the region’s increasing vulnerability to external food supply fluctuations. The findings strongly signal that current trends are insufficient and necessitate urgent and proactive policy interventions. To address this, practical policy recommendations include promoting water-saving technologies, enhancing regional cooperation, and strategically utilizing the international grain trade to ensure regional food security. Full article
(This article belongs to the Topic Food Security and Healthy Nutrition)
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16 pages, 3713 KiB  
Article
Synergistic Alleviation of Saline–Alkali Stress and Enhancement of Selenium Nutrition in Rice by ACC (1-Aminocyclopropane-1-Carboxylate) Deaminase-Producing Serratia liquefaciens and Biogenically Synthesized Nano-Selenium
by Nina Zhu, Xinpei Wei, Xingye Pan, Benkang Xie, Shuquan Xin and Kai Song
Plants 2025, 14(15), 2376; https://doi.org/10.3390/plants14152376 - 1 Aug 2025
Viewed by 179
Abstract
Soil salinization and selenium (Se) deficiency threaten global food security. This study developed a composite bioinoculant combining ACC deaminase-producing Serratia liquefaciens and biogenically synthesized nano-selenium (SeNPs) to alleviate saline–alkali stress and enhance Se nutrition in rice (Oryza sativa L.). A strain of [...] Read more.
Soil salinization and selenium (Se) deficiency threaten global food security. This study developed a composite bioinoculant combining ACC deaminase-producing Serratia liquefaciens and biogenically synthesized nano-selenium (SeNPs) to alleviate saline–alkali stress and enhance Se nutrition in rice (Oryza sativa L.). A strain of S. liquefaciens with high ACC deaminase activity was isolated and used to biosynthesize SeNPs with stable physicochemical properties. Pot experiments showed that application of the composite inoculant (S3: S. liquefaciens + 40 mmol/L SeNPs) significantly improved seedling biomass (fresh weight +53.8%, dry weight +60.6%), plant height (+31.6%), and root activity under saline–alkali conditions. S3 treatment also enhanced panicle weight, seed-setting rate, and grain Se content (234.13 μg/kg), meeting national Se-enriched rice standards. Moreover, it increased rhizosphere soil N, P, and K availability and improved microbial α-diversity. This is the first comprehensive demonstration that a synergistic bioformulation of ACC deaminase PGPR and biogenic SeNPs effectively mitigates saline–alkali stress, enhances soil fertility, and enables safe Se biofortification in rice. Full article
(This article belongs to the Special Issue Nanomaterials in Plant Growth and Stress Adaptation—2nd Edition)
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22 pages, 3023 KiB  
Article
Improving Grain Safety Using Radiation Dose Technologies
by Raushangul Uazhanova, Meruyert Ametova, Zhanar Nabiyeva, Igor Danko, Gulzhan Kurtibayeva, Kamilya Tyutebayeva, Aruzhan Khamit, Dana Myrzamet, Ece Sogut and Maxat Toishimanov
Agriculture 2025, 15(15), 1669; https://doi.org/10.3390/agriculture15151669 - 1 Aug 2025
Viewed by 231
Abstract
Reducing post-harvest losses of cereal crops is a key challenge for ensuring global food security amid the limited arable land and growing population. This study investigates the effectiveness of electron beam irradiation (5 MeV, ILU-10 accelerator) as a physical decontamination method for various [...] Read more.
Reducing post-harvest losses of cereal crops is a key challenge for ensuring global food security amid the limited arable land and growing population. This study investigates the effectiveness of electron beam irradiation (5 MeV, ILU-10 accelerator) as a physical decontamination method for various cereal crops cultivated in Kazakhstan. Samples were irradiated at doses ranging from 1 to 5 kGy, and microbiological indicators—including Quantity of Mesophilic Aerobic and Facultative Anaerobic Microorganisms (QMAFAnM), yeasts, and molds—were quantified according to national standards. Experimental results demonstrated an exponential decline in microbial contamination, with a >99% reduction achieved at doses of 4–5 kGy. The modeled inactivation kinetics showed strong agreement with the experimental data: R2 = 0.995 for QMAFAnM and R2 = 0.948 for mold, confirming the reliability of the exponential decay models. Additionally, key quality parameters—including protein content, moisture, and gluten—were evaluated post-irradiation. The results showed that protein levels remained largely stable across all doses, while slight but statistically insignificant fluctuations were observed in moisture and gluten contents. Principal component analysis and scatterplot matrix visualization confirmed clustering patterns related to radiation dose and crop type. The findings substantiate the feasibility of electron beam treatment as a scalable and safe technology for improving the microbiological quality and storage stability of cereal crops. Full article
(This article belongs to the Section Agricultural Product Quality and Safety)
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19 pages, 300 KiB  
Review
Sprouted Grains as a Source of Bioactive Compounds for Modulating Insulin Resistance
by Yan Sun, Caiyun Li and Aejin Lee
Appl. Sci. 2025, 15(15), 8574; https://doi.org/10.3390/app15158574 - 1 Aug 2025
Viewed by 296
Abstract
Sprouted grains are gaining attention as a natural and sustainable source of bioactive compounds with potential benefits in managing insulin resistance (IR), a hallmark of obesity-related metabolic disorders. This review aims to synthesize current findings on the biochemical changes induced during grain germination [...] Read more.
Sprouted grains are gaining attention as a natural and sustainable source of bioactive compounds with potential benefits in managing insulin resistance (IR), a hallmark of obesity-related metabolic disorders. This review aims to synthesize current findings on the biochemical changes induced during grain germination and their relevance to metabolic health. We examined recent in vitro, animal, and human studies focusing on how germination enhances the nutritional and functional properties of grains, particularly through the synthesis of compounds such as γ-aminobutyric acid, polyphenols, flavonoids, and antioxidants, while reducing anti-nutritional factors. These bioactive compounds have been shown to modulate metabolic and inflammatory pathways by inhibiting carbohydrate-digesting enzymes, suppressing pro-inflammatory cytokines, improving redox balance, and influencing gut microbiota composition. Collectively, these effects contribute to improved insulin sensitivity and glycemic control. The findings suggest that sprouted grains serve not only as functional food ingredients but also as accessible dietary tools for preventing or alleviating IR. Their role in delivering multiple bioactive molecules through a simple, environmentally friendly process highlights their promise in developing future nutrition-based strategies for metabolic disease prevention. Full article
(This article belongs to the Special Issue New Insights into Bioactive Compounds)
21 pages, 4228 KiB  
Article
The Combined Effect of Caseinates, Native or Heat-Treated Whey Proteins, and Cryogel Formation on the Characteristics of Kefiran Films
by Nikoletta Pouliou, Eirini Chrysovalantou Paraskevaidou, Athanasios Goulas, Stylianos Exarhopoulos and Georgia Dimitreli
Molecules 2025, 30(15), 3230; https://doi.org/10.3390/molecules30153230 - 1 Aug 2025
Viewed by 228
Abstract
Kefiran, the extracellular polysaccharide produced from the Generally Recognized as Safe (GRAS) bacteria in kefir grains, with its well-documented functional and health-promoting properties, constitutes a promising biopolymer with a variety of possible uses. Its compatibility with other biopolymers, such as milk proteins, and [...] Read more.
Kefiran, the extracellular polysaccharide produced from the Generally Recognized as Safe (GRAS) bacteria in kefir grains, with its well-documented functional and health-promoting properties, constitutes a promising biopolymer with a variety of possible uses. Its compatibility with other biopolymers, such as milk proteins, and its ability to form standalone cryogels allow it to be utilized for the fabrication of films with improved properties for applications in the food and biomedical–pharmaceutical industries. In the present work, the properties of kefiran films were investigated in the presence of milk proteins (sodium caseinate, native and heat-treated whey proteins, and their mixtures), alongside glycerol (as a plasticizer) and cryo-treatment of the film-forming solution prior to drying. A total of 24 kefiran films were fabricated and studied for their physical (thickness, moisture content, water solubility, color parameters and vapor adsorption), mechanical (tensile strength and elongation at break), and optical properties. Milk proteins increased film thickness, solubility and tensile strength and reduced water vapor adsorption. The hygroscopic effect of glycerol was mitigated in the presence of milk proteins and/or the application of cryo-treatment. Glycerol was the most effective at reducing the films’ opacity. Heat treatment of whey proteins proved to be the most effective in increasing film tensile strength, reducing, at the same time, the elongation at break, while sodium caseinates in combination with cryo-treatment resulted in films with high tensile strength and the highest elongation at break. Cryo-treatment, carried out in the present study through freezing followed by gradual thawing of the film-forming solution, proved to be the most effective factor in decreasing film roughness. Based on our results, proper selection of the film-forming solution composition and its treatment prior to drying can result in kefiran–glycerol films with favorable properties for particular applications. Full article
(This article belongs to the Special Issue Development of Food Packaging Materials)
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30 pages, 4804 KiB  
Article
Deep Storage Irrigation Enhances Grain Yield of Winter Wheat by Improving Plant Growth and Grain-Filling Process in Northwest China
by Xiaodong Fan, Dianyu Chen, Haitao Che, Yakun Wang, Yadan Du and Xiaotao Hu
Agronomy 2025, 15(8), 1852; https://doi.org/10.3390/agronomy15081852 - 31 Jul 2025
Viewed by 246
Abstract
In the irrigation districts of Northern China, the flood resources utilization for deep storage irrigation, which is essentially characterized by active excessive irrigation, aims to have the potential to mitigate freshwater shortages, and long-term groundwater overexploitation. It is crucial to detect the effects [...] Read more.
In the irrigation districts of Northern China, the flood resources utilization for deep storage irrigation, which is essentially characterized by active excessive irrigation, aims to have the potential to mitigate freshwater shortages, and long-term groundwater overexploitation. It is crucial to detect the effects of irrigation amounts on agricultural yield and the mechanisms under deep storage irrigation. A three-year field experiment (2020–2023) was conducted in the Guanzhong Plain, according to five soil wetting layer depths (RF: 0 cm; W1: control, 120 cm; W2: 140 cm; W3: 160 cm; W4: 180 cm) with soil saturation water content as the irrigation upper limit. Results exhibited that, compared to W1, the W2, W3, and W4 treatments led to the increased plant height, leaf area index, and dry matter accumulation. Meanwhile, the W2, W3, and W4 treatments improved kernel weight increment achieving maximum grain-filling rate (Wmax), maximum grain-filling rate (Gmax), and average grain-filling rate (Gave), thereby enhancing the effective spikes (ES) and grain number per spike (GS), and thus increased wheat grain yield (GY). In relative to W1, the W2, W3, and W4 treatments increased the ES, GS, and GY by 11.89–19.81%, 8.61–14.36%, and 8.17–13.62% across the three years. Notably, no significant difference was observed in GS and GY between W3 and W4 treatments, but W4 treatment displayed significant decreases in ES by 3.04%, 3.06%, and 2.98% in the respective years. The application of a structural equation modeling (SEM) revealed that deep storage irrigation improved ES and GS by positively regulating Wmax, Gmax, and Gave, thus significantly increasing GY. Overall, this study identified the optimal threshold (W3 treatment) to maximize wheat yields by optimizing both the vegetative growth and grain-filling dynamics. This study provides essential support for the feasibility assessment of deep storage irrigation before flood seasons, which is vital for the balance and coordination of food security and water security. Full article
(This article belongs to the Section Water Use and Irrigation)
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