Search Results (243)

Apple harvesting faces increasing challenges owing to rising labor costs and the limited seasonal workforce availability, highlighting the need for robotic harvesting solutions in precision agriculture. This study presents a 6-DOF robotic arm system designed for harvesting in dwarf hedge-planted orchards, featuring a lightweight perception module, a task-adaptive motion planner, and an adaptive soft gripper. A lightweight approach was introduced by integrating the Faster module within the C2f module of the You Only Look Once (YOLO) v8n architecture to optimize the real-time apple detection efficiency. For motion planning, a Dynamic Temperature Simplified Transition Adaptive Cost Bidirectional Transition-Based Rapidly Exploring Random Tree (DSA-BiTRRT) algorithm was developed, demonstrating significant improvements in the path planning performance. The adaptive soft gripper was evaluated for its detachment and load-bearing capacities. Field experiments revealed that the direct-pull method at 150 mN·m torque outperformed the rotation-pull method at both 100 mN·m and 150 mN·m. A custom control system integrating all components was validated in partially controlled orchards, where obstacle clearance and thinning were conducted to ensure operation safety. Tests conducted on 80 apples showed a 52.5% detachment success rate and a 47.5% overall harvesting success rate, with average detachment and full-cycle times of 7.7 s and 15.3 s per apple, respectively. These results highlight the system’s potential for advancing robotic fruit harvesting and contribute to the ongoing development of autonomous agricultural technologies. Full article

This article addresses the impact of deficit irrigation on the agronomic and physiological performance of “Rojo Brillante” persimmon trees in a Mediterranean climate. It compares the effect of a sustained deficit irrigation (SDI; imposing water deficit uniformly throughout the entire crop cycle) strategy and two regulated deficit irrigation (RDI; enforcing a water deficit during the phenological phases that are less sensitive to water stress) strategies. Field trials were conducted from 2022 to 2024 at the Cajamar Experimental Center in Paiporta, Valencia, Spain. The trees respond to mild water stress reducing transpiration through stomatal closure. RDI resulted in modest irrigation water savings (11–16%), minimizing fruit drop, leading to an increased number of fruits per tree and a higher marketable yield, although this came at the cost of a reduced unit fruit weight. SDI achieved a 30% reduction in irrigation water usage without impacting on the marketable yield, but it also caused a decrease in unit fruit weight. RDI increased water productivity (yield obtained per amount of water applied) primarily through higher yields, while SDI improved productivity mainly by lowering the amount of irrigation water applied. Both irrigation strategies are recommended for cultivating “Rojo Brillante” persimmons. RDI is especially advisable in years with lower fruit loads as more intensive thinning may be necessary in years with higher fruit loads. Conversely, SDI is recommended in situations where water availability is limited. Full article

The large-scale disposal of loquat (Eriobotrya japonica Lindl.) flowers during fruit thinning represents a significant waste of bioactive resources. This study systematically evaluated how three processing methods—fresh (FS), heat-dried (HD), and freeze-dried (FD) treatments—affect the flavonoid composition and antioxidant capacity of loquat flower extracts, with the aim of developing value-added, sugar-free functional tea ingredients. Using UPLC-MS/MS and DPPH assays, we analyzed both pre-(FS/HD/FD) and post-extraction samples (FSP/HDP/FDP) to assess processing-specific metabolic signatures and extraction efficiency. The results revealed that heat-dried powder (HDP) exhibited the highest total flavonoid content and DPPH scavenging capacity (615.24 µg Trolox/g), attributed to enhanced release of stable compounds like quercetin. Freeze-dried powder (FDP) better preserved heat-sensitive flavonoids, such as catechin-(4α→8)-gallocatechin and naringenin, but showed lower overall antioxidant activity. Multivariate analysis confirmed distinct clustering patterns, with heat-drying favoring flavonoid extractability while freeze-drying maintained metabolic diversity. These findings demonstrate that processing methods significantly influence bioactive compound retention and functionality, with heat-drying offering optimal balance between yield and practicality for industrial applications. This work provides a scientific foundation for upcycling loquat flowers into standardized nutraceutical ingredients, addressing both agricultural waste reduction and the growing demand for natural functional foods. Full article

Peach fruit faces severe postharvest losses due to thin epidermis and susceptibility to Rhizopus stolonifer-induced soft rot. Chemical control risks residue and resistance issues, demanding eco-friendly alternatives. This study elucidated the mechanism by which trans-2-hexenal (E2H) mitigated postharvest soft rot caused by Rhizopus stolonifer in peach (Prunus persica cv. Hujing Milu) fruit. The results demonstrated that E2H treatment significantly delayed lesion expansion by 44.7% and disease incidence by 23.9% while effectively maintaining fruit quality by delaying firmness loss, reducing juice leakage, and suppressing malondialdehyde (MDA) accumulation. E2H treatment upregulated phenylpropanoid pathway gene expression, enhancing key phenylpropanoid metabolism enzymes activities (phenylalanine ammonia-lyase (PAL), cinnamate 4-hydroxylase (C4H), 4-coumarate-CoA ligase (4CL), polyphenol oxidase (PPO), peroxidase (POD)), leading to the increase of total phenolics by 7.9%. E2H treatment analysis revealed significant enhancements in both chitinolytic activity (CHI) and β-1,3-glucanase (GLU) activity by 85.7% and 12.9%, indicating potentiation of the enzymatic defense system. Concurrently, E2H treatment could improve the redox modulation capacity of peach fruits through promoting catalytic efficiency of redox-regulating enzymes, increasing the accumulation of ascorbic acid (AsA) by 8.1%, inhibiting the synthesis of dehydroascorbic acid (DHA) by 18.6%, as well as suppressing the biosynthesis of reactive oxygen species (ROS). These coordinated enhancements in pathogenesis-related proteins (CHI, GLU), phenylpropanoid metabolism activation, and antioxidant systems are strongly associated with E2H-induced resistance against Rhizopus stolonifer, though contributions from other factors may also be involved. Full article

Sugar Belle^® mandarin is considered tolerant to Huanglongbing (HLB); however, recent reports have raised concerns about its fruit quality, noting issues such as reduced fruit size, thin peel, poor coloration, decreased firmness, and suboptimal juice quality. Two-year field experiments were conducted to improve external and internal fruit characteristics through foliar application of potassium (K) in five-year-old Sugar Belle mandarin grown in Florida sandy soil. The experiment consisted of foliar K supply (17 kg/ha) via Potassium Nitrate (PN, 4.7 kg/ha N), Dipotassium Phosphate (DKP, 12.7 kg/ha P₂O₅), PN with boron (PNB, 0.84 kg/ha B) at different application times (May, July, September), including one-time Gibberellic acid spray (GA@10 mg/L) and control treatments. PN application during July (PN_J) or two applications of PN with B during May and July (PNB_MJ) resulted in a larger fruit size (>65 mm). Results showed that PN application before fall (May or July) resulted in a significantly thicker peel (2.3 mm), 1.15 fold more than the control and GA treatment. Fruit puncture resistance force was significantly higher (33.1 N) with GA treatment (p = 0.07), followed by PNB_MJ (32.6 N). Meanwhile, K spray positively influenced juice qualities and peel color, regardless of application time or source. However, GA treatment significantly reduced juice quality and peel color. These findings highlighted the benefits of foliar K supply as PN to improve fruit qualities in HLB-affected citrus grown in sandy soil. Full article

Xyloglucan endotransglucosylases/hydrolases (XTHs) are key enzymes involved in cell wall remodeling by modifying xyloglucan–cellulose networks, thereby influencing plant growth, development, and secondary cell wall formation. While the roles of XTHs have been extensively studied in primary and secondary growth, their functions in the formation and thickening of lignified nut shells remain largely unknown. Pecan (Carya illinoinensis), an economically important nut crop, develops a hard, lignified shell that protects the seed during fruit maturation. In this study, we performed a comprehensive genome-wide characterization of the XTH gene family in pecan and identified 38 XTH genes, which were categorized into four distinct phylogenetic groups. Structural analyses of the deduced proteins revealed conserved catalytic residues alongside divergent loop regions, suggesting functional diversification. Expression profiling across various tissues and among pecan cultivars with contrasting shell phenotypes indicated that specific XTH genes may play critical roles in shell structure formation. Moreover, gene regulatory networks in thin- and thick-shelled pecans provided new insights into the molecular mechanisms underlying shell development and thickness regulation. These findings lay a foundation for future genetic improvement strategies targeting nut shell traits in woody perennials. Full article

The successful introduction of new cultivars depends on the evaluation of complex parameters essential for the consumers, market, and fruit producers. A new scab-resistant apple cultivar, ‘Wuranda’ (SQ159/Natyra^®/Magic Star^® × Honeycrisp), recently introduced in Norway and managed under the name Fryd^©, is prone to biennial bearing. Therefore, one of the first tasks, investigated in Southwestern Norway by the Norwegian Institute of Bioeconomy Research, NIBIO-Ullensvang in 2021–2024, was the establishment of optimal crop load level based on the combination of productivity, fruit quality, and return bloom. The apple cultivar Fryd (‘Wuranda’) was propagated on ‘M.9’ rootstock and planted in 2019. The trial was performed in the same orchard for four consecutive years, starting three years after planting. Crop load level affected average fruit mass but had no impact on cv. Fryd fruit quality parameters at harvest such as blush, ground color, firmness, soluble solid content, or starch degradation. Fruit size variation was diminished by crop load regulation, and most fruits fell into 2–3 grading classes. Crop load, not the yield per tree, was the determining factor for the return bloom. The optimal crop load level depended on the orchard age. To guarantee a regular bearing mode of cv. Fryd planted on M.9 rootstock at a 3.5 × 1 m distance and trained as slender spindle, crop load of 5.5–6 fruits cm⁻² TCSA (trunk cross-sectional area) in the 3rd year, 7.5–8 fruits cm⁻² TCSA in the 4th year, and 6.5–7 fruits cm⁻² TCSA in the 5th year should be maintained. Full article

Thinned peach fruit represents a substantial source of polyphenols, primarily due to its early developmental stage. Utilizing ultrasound-assisted extraction optimized through a Box–Behnken design, we determined the optimal extraction parameters to be 45 min, 360 W, a liquid-to-solid ratio of 15:1 mL/g, and a temperature of 70 °C. Under these conditions, the total phenolic content (TPC) achieved was 1.12 g GAE/kg FW, with an extraction efficiency of 97.06%. Additionally, an extensive evaluation of 179 peach cultivars revealed that wild accessions possessed significantly higher polyphenol content, including TPC, total flavonoid content (TFC), and total anthocyanin content (TAC), alongside enhanced antioxidant activities as measured by ferric reducing antioxidant power (FRAP), 2,2′-Azino-di-3-ethylbenzthiazoline Sulfonic Acid (ABTS), and 2,2-Diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assays, in comparison to landraces and cultivated varieties. Notably, the wild accession ‘Gansu Peach 2’ exhibited the highest TPC (2.61 g GAE/kg FW), whereas the landrace ‘Early White Blossom Peach’ demonstrated the highest TFC (137.32 g RTE/kg FW), TAC (25.30 g PAE/kg FW), and antioxidant capacity. Additionally, as expected, significant positive correlations (0.73 < r < 0.96) were also observed between polyphenol components and antioxidant activities (p < 0.0001). This study establishes a foundational framework for the utilization of thinned peach fruit as valuable polyphenol-rich resources. Full article

In this study, the melon variety ‘Da Shetou’ was used as the test material, and pot cultivation was employed with soil collected from Da’an City to investigate the effects of biochar addition on melon yield and quality, rhizosphere soil physicochemical properties, and soil microbial community. The experiment was set up with five treatments: saline–alkali soil (B0), 1% biochar and 99% saline–alkali soil (B1), 3% biochar and 97% saline–alkali soil (B3), 5% biochar and 95% saline–alkali soil (B5), and 7% biochar and 93% saline–alkali soil (B7). This study found that the addition of 3% biochar increased the fruit yield of melons. Compared to the control, the soil bulk density was reduced by 4.99%, 8.66%, 1.77%, and 7.71% under the 1%, 3%, 5%, and 7% biochar treatments, respectively. Biochar addition increased organic matter, alkaline-hydrolyzable nitrogen, available phosphorus, and available potassium concentrations in the rhizosphere soil. Additionally, the total nitrogen, salt concentration, and exchangeable sodium percentage were also reduced. Compared to the B0 treatment, the concentrations of K⁺, Ca²⁺, and Mg²⁺ increased to varying degrees across different treatments, while the concentrations of Na⁺ and Cl⁻ decreased. The relative abundance of dominant bacterial phyla in the soil varied across different treatments. The dominant bacterial phyla included Proteobacteria, Actinobacteriota, Acidobacteriota, and a total of 10 others. The dominant fungal phyla included Ascomycota, Basidiomycota, Mortierellomycota, and a total of seven others. Redundancy analysis (RDA) identified key drivers. Available potassium in the rhizosphere soil of melons was the dominant factor influencing bacterial community composition at the phylum level. Soil bulk density, exchangeable sodium percentage, and total nitrogen were identified as the dominant factors influencing fungal community composition at the phylum level. This study confirmed that 3% biochar application synergistically regulated nutrient cycling and microbial functional groups, thereby enhancing yield of thin-skinned melons (yield increase: 45.22%). Full article

High quality natural zeolites may have insecticidal effects and could be used for pest control. We determined the mineralogical and chemical composition of four representative samples of zeolite-rich rocks (zeot1–zeot4) collected from north-eastern Greece and their oviposition deterrent effect for the olive fruit fly Bactrocera oleae (Rossi) (Diptera: Tephritidae). Samples zeot1–zeot4 contain 54–70 wt.% clinoptilolite (HEU-type zeolite) and are free of fibrous minerals. Regarding the chemical composition, samples zeot1–zeot4 contain SiO₂ between 64.29 (zeot4) and 68.03 wt.% (zeot3). The values of the sorption ability ranged from 134 to 195 meq/100 g, and the specific surface area ranged from 6.5 to 8.4 cm²/g. In addition, the concentration of toxic heavy metals (As, Cd, Cr, Cu, Hg, Ni, Pb, Se, V, and Zn) is very low and within the acceptable limits for the food sector. When females of the olive fruit fly had access to olive fruits treated with aqueous suspensions of zeot1–zeot4, a significant oviposition deterrent effect was observed. The highest oviposition deterrent effect was observed after the application of zeot3 on the olive fruits, i.e., the mean number of eggs laid by 5 females on the treated and non-treated (control) olive fruits after 8 days was 43.1 and 172.3, respectively. Among the tested zeolites, zeot3 had the highest levels of HEU-type zeolite (clinoptilolite), SiO₂, Si, and Ca and the strongest sorption ability and specific surface area. The zeolites oviposition deterrent effect found in our experiments can be attributed to the creation of a thin layer (hymen) of natural zeolite on the surface of the olive fruits which inhibits females landing and egg laying. The oviposition deterrent effect of high-quality Greek zeolites with unique characteristics, if verified with field experiments, could improve the effective control of the olive fruit fly. Full article

Grapes, one of the most delicious and refreshing fruits in the world, are a source of sugar, minerals, and vitamins. Summer pruning affects ripening, disease control, yield, and quality parameters by controlling the vine microclimate. In our study, leaf removal, fruit thinning, and cluster thinning and their combination were applied to the Alphonse Lavallée grape variety, aiming to improve yield, cluster, and berry characteristics. As a result of the applications, cluster and berry characteristics, SSC, pH, titratable acidity (TA), total phenolic content, antioxidant activity, and color parameters were examined. In our study, all summer pruning applications and their combinations caused increases in cluster and berry parameters (weight, length, and width) compared to the control. In addition to these, the SSC, pH, and maturity index increased and TA decreased. All these applications also increased berry detachment and skin rupture force, which have an important place in road resistance in table grape varieties. Significant improvements were also seen in the quality parameters of total phenolic content and antioxidant activity. In addition, there were increases in the lightness and chroma values that determine the fruit quality in table grapes. Considering all these data, the summer pruning applications we made had significant effects on yield and quality. It is thought that cutting a part of the clusters instead of the whole cluster will especially prevent the yield loss experienced in cluster thinning applications. Full article

Pollination is essential for producing temperate-zone tree fruits like apples (Malus × domestica). While traditionally considered insect-dependent, this view may result from orchard designs tailored to European honeybees. Previous research showed that low-seed apples could develop in insect exclusion nets, suggesting wind as an alternative pollinator. This study investigated the paternal origin of seeds and fruit set under nets compared to open canopies. Netted canopies of ‘Gala’, Fuji’, and ‘Honeycrisp’ set commercial fruit numbers without manual thinning. To determine the parental source of seeds, genotyping was performed using 16 SNP markers tailored for distinguishing apple cultivars, with primer design and genotyping conducted via the KASP™ system. Results showed significant genetic overlap between seeds from netted and non-netted fruits and nearby pollinizers, ruling out self-pollination. Netted canopies retained fruits with similar or fewer seeds compared to abscised fruits in open canopies, indicating fruit set depends on the population’s seed content rather than individual fruit seed count. These findings supporting the hypothesis that apple trees are adapted to utilize both wind and insect pollination. While wind pollination offers a sustainable approach, it requires adjustments in orchard design to ensure sufficient pollen transfer for reliable fertilization and yield. Full article

Introduction: Taiwan green propolis (TGP) is rich in prenylflavonoids and exhibits antioxidant, antibacterial, antiviral, and antitumour properties. It induces apoptosis in various cancer cells, making it a highly promising natural medicine. Although the health benefits and food applications of TGP are widely recognised, no study has explored its effects on Taiwan oral cancer cells (OECM1). This study investigated whether TGP induces apoptosis in OECM1 cells. Methods: High-performance liquid chromatography (HPLC), thin-layer chromatography, and liquid chromatography/mass spectrometry were used to identify the components in TGP and the fruit peel of Macaranga tanarius. The inhibitory activities of TGP dissolved in DMSO (TGP_DMSO) and encapsulated in food-grade zein nanoparticles (TGP_NP) against OECM1 cells were compared using MTT assays. The morphological changes, cell cycle analysis, and protein expression profiles of OECM1 cells after the TGP treatments were performed using microscopy, flow cytometry, and Western blot, respectively. Results: An MTT assay of TGP_DMSO-treated OECM1 cells suggested an IC₅₀ of 12.6 µg/mL, demonstrating that TGP_DMSO exhibits significant cytotoxicity. Subsequent MTT assays revealed TGP_NP’s cytotoxicity against OECM1 with an IC₅₀ of 11.6 µg/mL. Flow cytometry revealed that TGP_NP induced a cell arrest in S phase and DNA fragmentation. Western blotting analyses manifested an increase in Bax and cl-Casp9 and a decrease in Bcl2 and PARP. Conclusion: This study demonstrated that both TGP_DMSO and TGP_NP treatments induced apoptosis in OECM1 cells with a comparable IC₅₀. Notably, utilising edible zein as a nanoparticle carrier for TGP mitigates the cytotoxicity risk associated with DMSO, providing a novel and safe approach for cancer treatment. Full article

Composite edible coatings based on arabic gum with mango peel hydrocolloids and mango seed extracts were prepared and used to evaluate grape conservation. Hydroethanolic solutions were used for the obtention of mango seed extracts, by microwave-assisted extraction, with total phenolic compounds (5.48 and 9.85 GAE/g of extract) and antioxidant activity (<13.03 µmol Trolox/g of extract). The extracts were selected for the development of edible coatings. The rheological properties of edible coating solutions present a non-Newtonian behavior-type shear thinning fluid; the addition of extracts improves their viscoelastic properties, favoring their application into grapes. The coated grapes maintained physicochemical parameters, such as weight, pH, acidity, soluble solids, and color during the 15 days of storage. The results of this research offer the possibility of using by-products from fruit industries, especially mango, to obtain functional ingredients and their application in food systems, taking advantage of their biological activity. Full article

Autonomous navigation is key to improving efficiency and addressing labor shortages in the fruit industry. Semi-structured orchards, with straight tree rows, dense weeds, thick canopies, and varying light conditions, pose challenges for tree identification and navigation line extraction. Traditional 3D lidars suffer from a narrow vertical FoV, sparse point clouds, and high costs. Furthermore, most lidar-based tree-row-detection algorithms struggle to extract high-quality navigation lines in scenarios with thin trunks and dense foliage occlusion. To address these challenges, we developed a 3D perception system using a servo motor to control the rolling motion of a 2D lidar, constructing 3D point clouds with a wide vertical FoV and high resolution. In addition, a method for trunk feature point extraction and tree row line detection for autonomous navigation has been proposed, based on trunk geometric features and RANSAC. Outdoor tests demonstrate the system’s effectiveness. At speeds of 0.2 m/s and 0.5 m/s, the average distance errors are 0.023 m and 0.016 m, respectively, while the average angular errors are 0.272° and 0.146°. This low-cost solution overcomes traditional lidar-based navigation method limitations, making it promising for autonomous navigation in semi-structured orchards. Full article