Search Results (161)

The production of fermented sausages from poultry meat using traditional technologies and natural maturation conditions is a major challenge. The aim of this study was to identify indigenous microbiota with antilisterial activity from an innovative, additive-free, traditionally fermented chicken sausage. Isolates (n = 88) of lactic acid bacteria (LAB) were collected during maturation and subjected to MALDI-TOF mass spectrometry identification. The capacity to combat Listeria was screened against five strains using the agar well diffusion method in 63 selected LAB isolates. MALDI-TOF mass spectrometry identified four different LAB genera, namely Enterococcus, Lactococcus, Leuconostoc and Lactobacillus, the proportions of which differed significantly during the production phases (p < 0.001). Enterococcus faecalis was the most prevalent LAB species in the initial sausage dough. The presence of lactococci (Lactococcus lactis) and enterococci was detected during the 14- and 30-day ripening period and was gradually displaced by leuconostocs and lactobacilli. Lactobacilli appeared to be abundant during the central and late maturation phases, and consisted of only two species—Latilactobacillus sakei and Latilactobacillus curvatus. In total, 38 LAB isolates (60%) showed antilisterial activity toward at least one Listeria indicator strain. The proportions of antilisterial LAB differed significantly during sausage maturation. Inhibitory activity against all indicator Listeria was detected in the neutralized cell-free supernatants of five strains of Enterococcus faecalis, two L. sakei strains and one Leuconostoc mesenteroides strain. The antilisterial activity observed in the indigenous LAB revealed the possible role of L. sakei as a bioprotective culture, as well as the role of Ln. mesenteroides and E. faecalis as bacteriocin producers, for practical applications. Full article

Vertebrate molecular genetic research methods typically employ single genetic loci (monolocus markers) and those involving a variable number of loci (multilocus markers). The former often employ microsatellites that ensure accuracy in establishing inbreeding, tracking pan-generational dynamics of genetic parameters, assessing genetic purity, and facilitating genotype/phenotype correlations. They also enable the determination and identification of unique alleles by studying and managing marker-assisted breeding regimes to control the artificial selection of agriculturally important traits. Microsatellites consist of 2–6 nucleotides that repeat numerous times and are widely distributed throughout genomes. Their main advantages lie in their ease of use for PCR amplification, their known genome localization, and their incredible polymorphism (variability) levels. Robust lab-based molecular technologies are supplemented by high-quality statistics and bioinformatics and have been widely employed, especially in those instances when more costly, high throughput techniques are not available. Here, we consider that human and livestock microsatellite studies have been a “roadmap” for the genetics, breeding, and conservation of wildlife and rare animal breeds. In this context, we examine humans and other primates, cattle and other artiodactyls, chickens and other birds, carnivores (cats and dogs), elephants, reptiles, amphibians, and fish. Studies originally designed for mass animal production have thus been adapted to save less abundant species, highlighting the need for molecular scientists to consider where research may be applied in different disciplines. Full article

Untreated olive oil mill wastewater (OOMW) from conventionally farmed olives was used in bread production to create a new functional product. Two types of bread were developed with 50% OOMW (EXP-1) and 100% OOMW (EXP-2) replacing water. Two leavening processes were tested: sourdough inoculum (S) vs. biga-like inoculum (B), with controls (CTR) without OOMW addition. The doughs were monitored throughout the acidification process by measuring pH, total titratable acidity, and the development of key fermentative microorganisms. To assess the hygienic quality during fermentation, plate count techniques were employed. After baking, the breads were evaluated for various quality parameters, including weight loss, specific volume, crumb and crust colors, image analysis, and the presence of spore-forming bacteria. Volatile compounds released from the breads were identified using solid-phase microextraction coupled with gas chromatography–mass spectrometry (SPME-GC/MS). Polyphenolic compounds were analyzed via liquid chromatography–mass spectrometry (LC-MS). To assess the functional properties of the final products, the breads were homogenized with synthetic human saliva and subjected to in vitro digestion. OOMW did not significantly affect the growth of yeasts and lactic acid bacteria (LAB) or the acidification process. However, in terms of the specific volume and alveolation, breads from the S process and OOMW had poor quality, while those from the B process had better quality. Experimental breads (EXP_B-1 and EXP_B-2) contained higher levels of alcohols (especially ethanol and isobutyl alcohol), carbonyl compounds (like benzaldehyde), esters (such as ethyl caproate and ethyl caprylate), and terpenes. OOMW introduced phenolic compounds like hydroxytyrosol, coumaric acid, caffeic acid, and trans-hydroxycinnamic acid, which were absent in CTR_B breads. Functionalization of EXP_B-1 and EXP_B-2 breads was demonstrated by a 2.4- and 3.9-fold increase in Trolox equivalents, respectively. However, OOMW did not reduce post-prandial hyper-glycemia, as starch digestibility was similar between CTR_B and EXP_B breads. The sensory analysis, which focused solely on the visual, structural, and olfactory characteristics of the breads, excluding taste testing to prevent potential health risks from residual pesticides, showed a high appreciation for EXP_B-1 and EXP_B-2 breads, scoring higher than CTR_B in the overall assessment. Full article

Food contact polymers require thermochromic pigments to provide temperature-sensitive visual cues for consumer safety and product integrity. However, their susceptibility to ultraviolet (UV) degradation limits long-term application. This study investigates the UV resistance of food-grade thermochromic polypropylene blends under simulated indoor and outdoor UV exposure for 500 and 1000 h. Visual properties, colorimetric (CIE L*a*b*) measurements, mechanical testing (tensile and impact), and mass variation analysis were performed to assess photostability and material integrity. Exposure to UV led to progressive discoloration (ΔE*_ab up to 34.07) and significant mechanical deterioration. Tensile strain at break decreased by 48.67%, and notched impact strength dropped by 44.15% after 1000 h of UV exposure. No measurable mass loss occurred, indicating degradation was confined to surface-level oxidation rather than bulk material erosion or leaching. These findings highlight the need for optimal pigment loading and UV stabilization to extend the shelf life of thermochromic food packaging materials in light-exposed storage and retail environments. The study offers a framework for improving the long-term reliability of smart packaging in the food industry. This work uniquely integrates optical, mechanical, and mass loss analyses to evaluate thermochromic packaging degradation under extended UVA exposure. Full article

In line with the recommended European policy for a zero-waste crop supply chain, a lab-pilot optimisation process to valorise the by-products of industrially produced rapeseed meal (RM) was performed. Three batches of RM were first processed into ethanol-wash solutes (EWS) and then optimised (OEWS) by an ultrasound-assisted (UA) treatment. After direct analysis in real time–high resolution mass spectrometry (DART-HRMS) analysis, data were processed applying a partial least square–discriminant analysis (PLS-DA), which retrieved the 15 most discriminative ions able to characterise the biochemical changes during the ethanol-washing and UA optimisation process. The metabolomic fingerprinting of EWS and OEWS generated an accurate and well-defined 3D spatial clusterisation based on a restricted pool of informative bioactive compounds. A significantly higher relative abundance of sinapic, azelaic, and vernolic acids and a lower incidence of the oleic and palmitic fatty acids were detected in OEWS. DART-HRMS generated a vast amount of biochemical information in one single run, also demonstrating that its association with an untargeted multivariate statistical approach would be a valuable tool for revealing specific functional biomarkers. This would eventually enhance the circular and effective use of rapeseed residuals coming from this plant’s oilseed industry. Full article

The construction sector’s increasing eco-consciousness is driving the need for higher-performance wood-based engineered products from underutilized timber resources, such as small-diameter trees from hazardous fuel treatments of our forests. Strand-based products, including oriented strand board (OSB) and lumber (OSL), are widely used. However, hot-pressing during their manufacturing generates approximately 10% waste, which includes a substantial amount of resinated strands that are landfilled. The huge potential of using strand-based products has led to many studies and growing interest in strand-based three-dimensional sandwich panels that can be used as wall, floor, or roofing panels. As the market grows, understanding the recyclability of these resinated strands becomes crucial. This study investigates the feasibility of using re-resinated waste strands that were collected during lab-scale production of strand-based panels. Results demonstrate significant improvements in dimensional stability, mechanical properties, and fire resistance. Specifically, recycling increased internal bond strength, flexural strength, time to ignition, time to flameout, mass loss, and time to peak heat release rate by 107%, 44%, 58%, 35%, 51%, and 27%, respectively, and helped decrease water absorption and thickness swell by 51% and 58%, respectively. Full article

Folate deficiency is a widespread nutritional issue, and biofortifying dairy products through lactic acid bacteria (LAB) is a promising strategy to enhance natural folate levels. This study aimed to develop a reliable method for selecting Streptococcus thermophilus and Lactobacillus delbrueckii subsp. bulgaricus strains with enhanced folate production for use as functional starter cultures. Initially, a traditional microbiological assay (MA) was used to measure folate production in 36 LAB strains isolated from fermented milks. Due to MA’s limitations, an untargeted and semi-quantitative method combining ultra-high-performance liquid chromatography (UHPLC) with high-resolution mass spectrometry (HRMS) was developed for a more comprehensive folate screening. The MA showed higher folate production in S. thermophilus strains (309–639 µg/L) compared to L. delbrueckii subsp. bulgaricus (up to 48 µg/L). Subsequently, nine selected LAB strains were further analyzed using the UHPLC-HRMS approach, which enabled the identification and semi-quantification of six folate metabolites, namely dihydrofolate, tetrahydrofolate (THF), 10-formyl-THF, 5,10-methenyl-THF, 5,10-methylene-THF, and 5-methyl-THF. Lab-scale yogurt production using the top-performing strains, as identified through the HRMS method, demonstrated an increase in folate content over a 14-day shelf life. These findings revealed the potential of UHPLC-HRMS as a high-throughput alternative method for folates detection, offering a promising tool for screening folate-enhanced LAB strains for biofortification. Full article

Lactic acid bacteria (LAB) fermentation has been claimed as an effective way of modifying the sensory properties of plant-based foods. However, not much has been published on the influence of different LAB strains on the flavour of the volatile organic compounds (VOCs) produced. Using a defined medium (DM) and proton-transfer-reaction time-of-flight mass spectrometry (PTR-ToF-MS), we assessed the VOCs produced by seven LAB strains, Levilactobacillus brevis WLP672 (LB672), Lactobacillus delbrueckii WLP677 (LD677), Pediococcus damnosus WLP661 (PD661), Lactiplantibacillus plantarum LP100 (LP100), Pediococcus pentosaceus PP100 (PP100), Pediococcus damnosus 5733 (PD5733), and Lentilactobacillus buchneri 5335 (LU5335), at three time points during fermentation (0, 7, and 14 days) at either 25 or 35 °C. Significant variations in VOC production were observed among LAB strains, growing in the same DM composition at either 25 °C or 35 °C. Specifically, the concentration of m/z 87.043 (t.i. diacetyl) was significantly (p < 0.05) higher at 7 days of fermentation at 35 °C by LP100, followed by PP100 at 35 °C and PD661 at 25 °C compared to the other strains at either 25 or 35 °C. The concentration of m/z 115.112 (t.i. 2-heptanone) was significantly (p < 0.05) higher at 7 days of fermentation at either 25 or 35 °C by LP100 compared to the other strains at all temperature and time points. The concentration of m/z 49.011 (t.i. methanethiol) was significantly (p < 0.05) higher after 7 days of fermentation at 35 °C by LB672 compared to the other strains at either 25 or 35 °C. The concentration of m/z 71.085 (t.i. 3-methyl butanol) was significantly (p < 0.05) higher after 7 days of fermentation at either 25 or 35 °C by PD661, LU5335, or PD5733 compared to the other strains studied. A notable increase in specific VOC concentrations was observed at 35 °C compared to 25 °C. This research demonstrates that LAB strains generate distinct VOC profiles in a DM based on strains and fermentation conditions. Therefore, this knowledge provides a basis for controlling and enhancing flavour in plant-based fermentations. Full article

Northeastern Thai ethnic foods are celebrated for their health benefits yet remain largely underexplored. This study assessed the antioxidant and cytotoxic properties of two ready-to-eat pastes—Jaew Hon (JH) and Gang Om (GO)—produced using laboratory (LAB) and industrial original equipment manufacturer (OEM) methods. Evaluations were conducted using 2,2-Diphenyl-1-picrylhydrazyl (DPPH), ferric reducing antioxidant power (FRAP), total phenolic content (TPC), and total flavonoid content (TFC) assays alongside the 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay for cytotoxicity. Physicochemical analyses revealed that JH OEM had the highest total dissolved solids (11.57°Brix) and water activity (0.91), while GO OEM exhibited the highest pH (5.28) and lightness (L* 31.43). Antioxidant results showed JH LAB outperformed in DPPH scavenging (96.25 mg AAE/100 g) and TPC (433.5 mg GAE/100 g), whereas GO OEM achieved the highest TFC (345.57 mg QE/100 g). Volatile compound profiling by Gas Chromatography–Mass Spectrometry (GC-MS) indicated distinct aroma profiles between LAB and OEM samples. Moreover, MTT assays revealed stronger cytotoxic effects for OEM products; specifically, GO OEM achieved 71.88% maximum inhibition and an IC50 of 276.10 µg/mL against HT-29 cells. Colony formation assays confirmed GO OEM’s significant antiproliferative activity, and gene expression analysis demonstrated upregulation of pro-apoptotic markers (Bax, Caspase-3) alongside downregulation of NF-κB p65, Cyclin D1, and MMP-9. Overall, these findings suggest that industrially produced GO and JH pastes hold promise as functional foods, integrating traditional culinary practices with modern production techniques. These findings lay the foundation for future research focused on uncovering bioactive mechanisms, optimizing processing methods, and confirming health benefits through in vivo studies. Full article

Phosphorus is essential to environmental systems because it affects both agricultural productivity and ecological balance. Since it contributes to eutrophication and pollution problems, its existence in a variety of environmental matrices, including soil, water, and air, necessitates precise and effective determination methods for monitoring and managing its levels. This review paper provides an extensive overview of the latest advancements in analytical techniques for measuring phosphorus in environmental samples. We investigate sophisticated spectroscopic, chromatographic, and electrochemical techniques in addition to conventional approaches like colorimetric analysis. Innovative techniques such as mass spectrometry (MS), X-ray fluorescence (XRF) spectrometry, and nuclear magnetic resonance (NMR) spectroscopy are also highlighted in this study, along with newly developed technologies such as biosensors, lab-on-a-chip devices, and nanotechnology-based techniques. Real-time and field-deployable monitoring technologies are also covered, with a focus on their advantages and usefulness. Among the techniques reviewed, XRF and colorimetry methods have proven to be the most reliable due to their precision, cost-effectiveness, and adaptability for different sample matrices. While emerging spectroscopic and electrochemical techniques offer promising alternatives, further validation and standardization are needed for routine environmental monitoring. Future research should focus on integrating automated and high-throughput techniques to enhance monitoring capabilities further. Full article

Cemented Sand, Gravel, and Rock (CSGR) dams have traditionally used either Conventional Vibrated Concrete (CVC) or Grout-Enriched Roller Compacted Concrete (GERCC) for protective and seepage control layers in low- to medium-height dams. However, these methods are complex, prone to interference, and uneconomical due to significant differences in the expansion coefficient, elastic modulus, and hydration heat parameters among CSGR, CVC, and GERCC. This complexity complicates quality control during construction, leading to the development of Grout-Enriched Vibrated Cemented Sand, Gravel, and Rock (GECSGR) as an alternative. Despite its potential, GECSGR has limited use due to concerns about freeze–thaw resistance. This project addresses these concerns by developing an air-entrained GECSGR grout formulation and construction technique. The study follows a five-phase approach: mix proportioning of C₁₈₀6 CSGR; optimization of the grout formulation; determination of grout addition rate; evaluation of small-scale lab samples of GECSGR; and field application. The results indicate that combining 8–12% of 223 kg/m³ cement grout with 2–2.23 kg/m³ of admixtures, mud content of 15%, a marsh time of 26–31 s. and a water/cement ratio of 0.5–0.6 with the C₁₈₀6 parent CSGR mixture achieved a post-vibration in situ air content of 4–6%, excellent freeze–thaw resistance (F300: mass loss <5% or initial dynamic modulus ≥60%), and permeability resistance (W12: permeability coefficient of 0.13 × 10⁻¹⁰ m/s). The development of a 2-in-1 slurry addition and vibration equipment eliminated performance risks and enhanced efficiency in field applications, such as the conversion of the C₁₈₀4 CSGR mixture into air-entrained GECSGR grade C9015W6F50 for the 2.76 km Qianwei protection dam. Economic analysis revealed that the unit cost of GECSGR production is 18.3% and 6.33% less than CVC and GERCC, respectively, marking a significant advancement in sustainable cement-based composite materials in the dam industry. Full article

Lactic acid bacteria (LAB) are widely used to produce various food products, adding flavor, texture, and health benefits. The bacteria are commonly grown on expensive nutrients like glucose, sucrose, and yeast extracts, which makes them commercially unappealing. In the current study, Lactobacillus acidophilus TISTR 1338 culture was studied using spent cell yeast as a nitrogen source and molasses as a carbon source. The drying process used to create starter cultures of Lactobacillus acidophilus TISTR 1338 was vacuum drying. After vacuum drying, this bacterium had a survival rate of 8.08 log CFU/g. The dried strain survived for four months at 37 °C. With wasted cells at 0.5%, molasses concentration at 11% at 2.14 109 CFU/mL at 22 h, precise growth rate at 0.39 h⁻¹, and yield cell mass at 1.67 1011 CFU/g sugar, yeast produced the maximum cell mass. The lower viability of the tested strain was induced by a higher temperature during this prolonged storage. Meanwhile, dehydrated starter culture was subjected to accelerated storage testing at 50, 60, and 70 °C. To determine the vacuum-dried Lactobacillus acidophilus TISTR 1338′s long-term storage viability, a temperature-dependent prophecy model was created. Molasses and spent cell yeast serve as promising carbon and nitrogen sources when optimized conditions are employed. The study also suggests that vacuum drying is a promising method for producing dried cells suitable for non-refrigerated storage conditions. Full article

Ensiling is a promising low-cost preservation approach that allows for a year-round supply of kelp feedstock for biofuel production via anaerobic digestion. In this study, farm-grown kelps of known age were ensiled with and without the addition of lactic acid bacterial (LAB) inoculant for a duration of up to one year in order to test long-term storage suitability. The study looked at the impacts of different bacterial inoculums on the chemical and microbial composition over the duration of storage. Significant fluctuations in the pH were observed during ensiling, leading to some cases of secondary fermentation and a loss of volatile components; however, over 12 months, the total mass loss was <2% on average. Biochemical compositional changes occurred in the silage over a period of 12 months, but protein, lipid and carbohydrate content remained suitable for biogas production. Microbial analysis showed variability in the bacterial distribution between the ensiled samples that was coincident with pH variability. Despite this variability, the bacterial communities underwent a succession with a selection for ensilage bacteria and drop in spoilage organisms. This shift supports the viability of this ensiled material for future usage. The impact of ensiling on bioenergy production through anaerobic digestion is explored in the second part of this two-part paper. Full article

Conventional lubricant testing methods focus on lab-scale constant contact conditions, which cannot represent the scenarios in actual hot-stamping processes. In recent studies, the concept of the ‘digital characteristics (DC)’ of metal forming has been proposed by unveiling the intrinsic nature of the specific forming, which presents a timely solution to address this challenge. In this work, the transient behaviours of three dedicated lubricants during the hot stamping of AA6111 material were investigated considering the effects of various contact conditions using an advanced friction testing system, and the interactive friction modelling was established accordingly. The lubricant limit diagram (LLD) of each lubricant was then generated to quantitatively evaluate the lubricant performance following the complex tool–workpiece interactions based on the tribological DCs, and a detailed investigation on the lubricant failure regions was conducted based on the interactive friction modelling. Finally, the industrial application index (IAI) was proposed and defined as a comprehensive evaluation of lubricant applications in the industry, and the most suitable lubricant was identified among the three candidates for mass production. Full article

The use of non-degradable plastic mulch has become an essential agricultural practice for increasing crop yields, but continued use has led to contamination problems and in some cropping areas decreases in agricultural productivity. The subsequent emergence of biodegradable plastic mulches is a technological solution to these issues, so it is important to understand how different soil characteristics and field management strategies will affect the rate at which these new materials degrade in nature. In this work, a series of lab-scale hydrolytic degradation experiments were conducted to determine how different soil characteristics (type, pH, microbial community composition, and particle size) affected the degradation rate of a sprayable polyester–urethane–urea (PEUU) developed as a biodegradable mulch. The laboratory experiments were coupled with long-term, outdoor, soil degradation studies, carried out in Clayton, Victoria, to build a picture of important factors that can control the rate of PEUU degradation. It was found that temperature and acidity were the most important factors, with increasing temperature and decreasing pH leading to faster degradation. Other important factors affecting the rate of degradation were the composition of the soil microbial community, the mass loading of PEUU on soil, and the degree to which the PEUU was in contact with the soil. Full article