Search Results (1,526)

Microplastic contamination in food systems has emerged as a growing concern for food safety and public health. The presence of microplastics in raw milk may represent a potential exposure pathway for both animals and humans. This study investigated the occurrence and characteristics of microplastics in raw milk collected from smallholder dairy farms in Northeastern Thailand. Hand-milked raw milk and bulk tank milk samples were obtained from ten farms and analyzed for microplastic contamination. Suspected microplastic particles were identified and quantified using stereomicroscopy and characterized according to their shape, color, and size, while polymer composition was confirmed by Fourier Transform Infrared Spectroscopy. Microplastics were detected in both hand-milked and bulk tank milk samples, with fiber-shaped particles being the most frequently observed. The majority of detected particles were 0.05–0.15 mm in size and predominantly yellow in color. Polymer analysis revealed that Polydimethylsiloxane, followed by a semi-synthetic composite of Elastane and Rayon. These findings demonstrate that microplastics can be present in raw milk produced by smallholder dairy farms, highlighting the need for improved farm management and milk-handling practices to reduce contamination risks. From a One Health perspective, reducing plastic use and enhancing hygiene during milking and milk storage may help protect animal health, food safety, and consumer well-being. Full article

Leather has been a commodity since ancient times, when primitive men hunted animals for food and used their hides and skins for clothes and tents. Nowadays, the tanning process is highly industrialised. The chromium tanning is the most widely used because it produces high-quality leather despite its serious environmental impacts. The purpose of this study is to analyse the environmental impact of an Indian company that carries out post-tanning operations on bovine hides, that is to say, from the so-called wet-blue to finished crust. To do this, the Life Cycle Assessment (LCA) is implemented using the primary data provided by the company. The analysis has been carried out by the OpenLCA software, and 16 environmental impact categories have been evaluated. The results show that the processes for producing fuel (coal and diesel oil) and chromium(III) salts are the main contributors to the environmental impact for nearly all categories. These types of impacts are upstream, whereas the operations carried out by the company have impacts on the climate change category, due to the use of fossil fuels in the production process. Therefore, the direct action that the company could take is the substitution of fuel to produce energy with a renewable energy source. The comparison of these results with the whole tanning process present in the software confirms the limited impact of the post-tanning. At last, the results also evidence the methodological value of Life Cycle Assessment, which can be used to show what can be improved in one installation to reduce its environmental impact. Full article

The escalating threat of antibiotic resistance, particularly from Staphylococcus aureus (S. aureus), has become a critical challenge in both public health and animal husbandry. The extensive use of conventional antibiotics in livestock production accelerates the emergence of resistant strains, heightening risks to food safety and human health. Although plant-derived bioactive compounds are increasingly recognized as promising alternatives to synthetic antimicrobials, the mechanisms underlying their efficacy—and the potential for synergistic action among different plant parts—remain poorly understood. In particular, the antibacterial interactions among extracts from different tissues of Cyperus esculentus L. (C. esculentus), a plant rich in flavonoids and phenolics, have yet to be systematically evaluated. Here, we investigated the antibacterial properties and mechanisms of ethanol extracts from the tubers, stems–leaves and their mixture of C. esculentus against S. aureus. Using Oxford cup diffusion assays, scanning electron microscopy (SEM), bacterial growth kinetics, and untargeted metabolomics, we assessed both phenotypic inhibition and metabolic disruption. The mixed extract exhibited the strongest antibacterial effect, producing a 26.15 mm inhibition zone—approximately 7% greater than that of single-part extracts—and induced cell wall rupture and disintegration as observed by SEM. Growth curve analyses revealed time-dependent bacterial suppression, while metabolomic profiling identified 845 differential metabolites, indicating disturbances in amino acid, lipid, and nucleotide metabolism. Flavonoids such as acacetin, diosmetin, naringenin, and silybin A were identified as principal active compounds contributing to these effects. Full article

Klebsiella pneumoniae is commonly known to cause a vast range of community-acquired or nosocomial infections. The isolation of K. pneumoniae has also been noted in diseased food-producing animals, including swine. The main goals of this study were to document clinical manifestation of a septicaemia outbreak in suckling piglets due to K. pneumoniae ST25 and provide molecular characterisation of the isolates. For the purpose of this investigation, 13 dead suckling piglets with cyanosis were selected. All the isolates obtained from affected lungs were susceptible to apramycin, ceftiofur, gentamycin, neomycin, and spectinomycin, presented intermediate susceptibility to florfenicol, and were resistant to other tested antibiotics. Histopathological examination of lungs, kidneys, and livers revealed lesions typical of septicaemia. MLST analysis of the isolates demonstrated a complex metabolic profile of the bacteria with genes attributable to the hypervirulent phenotype. To the best of our knowledge, we documented the first outbreak of K. pneumoniae septicaemia in suckling piglets reared in Poland. Full article

Bioactive compounds in food contribute to reducing the risk of developing colon cancer by modulating the gut microbiota. We have recently demonstrated that garambullo (Myrtillocactus geometrizans), an endemic fruit of Mexico rich in bioactive compounds, attenuates aberrant crypt foci in an animal model. However, its potential to modulate the gut microbiota is unknown. The main objective of this study was to evaluate whether its consumption modulates colon carcinogenesis by altering the microbiota in an in vivo model induced by azoxymethane and dextran sulfate sodium (AOM/DSS). Fecal samples were collected from twelve male Sprague-Dawley rats and analyzed for microbiota composition after 0, 8, and 16 weeks of treatment with saline (control), AOM/DSS, garambullo (G), or residue of garambullo (RG) with AOM/DSS (G+AOM/DSS and RG+AOM/DSS, respectively). Characterization of the microbiome was based on the conserved region of the 16S rRNA V3-V4 gene, and analyzed by the ZymoBIOMICS’ Targeted Metagenomics Sequencing (Zymo Research) service. In an animal model induced with AOM/DSS for 8 weeks, consumption of G and its residue increased the bacterial genera Shuttleworthiia, Subdoligranulum, Lactobacillus, Faecalibacterium, and Alloprevotella (p < 0.05). Consumption of G and its residue allowed the proliferation of bacteria that produce short-chain fatty acids and are associated with protective mechanisms of the colon. Full article

Microbial fermentation is a key biotechnological tool for producing bioactive metabolites such as alkaloids, carotenoids, essential oils, and phenolic compounds, among others, with applications in human health, agriculture, and food industries. This review comprehensively reviews recent information on the synthesis of valuable compounds and enzymes through fermentation processes. Here, we discuss the advantages of the different types of fermentation, such as submerged and solid-state fermentation, in optimizing metabolite production by bacteria, fungi, and yeast. The role of microbial metabolism, enzymatic activity, and fermentation conditions in enhancing the bioavailability and functionality of these compounds is discussed. Integrating fermentation with emerging biotechnologies, including metabolic engineering, further enhances yields and specificity. The potential of microbial-derived bioactive compounds in developing functional foods, pharmaceuticals, and eco-friendly agricultural solutions positions fermentation as a pivotal strategy for future biotechnological advancements. Therefore, microbial fermentation is a sustainable tool to obtain high-quality metabolites from different sources that can be used in agriculture, animal, and human health. Full article

Shiga toxin-producing Escherichia coli (STEC) is a zoonotic pathogen of public health concern, requiring a One Health approach to clarify its transmission and distribution. However, its prevalence and genomic characteristics in livestock and companion animals remain underexplored in low-income countries. We investigated prevalence and genomic features of STEC in animals in western Ghana, representing the first genomic report of STEC in Ghana. Fecal samples (97) were collected from goats (n = 33), sheep (n = 33), dogs (n = 30), and a cat (n = 1), with STEC detected in 12.1% of goats and sheep samples. Whole-genome sequencing identified serotypes O38:H26, O43:H2, and O157:H7. stx1c and stx2b genes were detected in O38:H26 and O43:H2, whereas stx2c and key virulence genes (chuA, eae, esp, nle, tir, and toxB) were exclusively found in O157:H7. Phylogenetic analysis revealed that O38:H26 isolates form a cluster closely related to clinical strains from the UK. O43:H2 isolates exhibited diverse stx profiles, linking animal, environmental, and clinical strains from North America and the UK. O157:H7 isolates were genetically similar to European clinical and food-derived strains, suggesting that goats and sheep are important STEC reservoirs in Ghana, offering data for public health risk assessment and effective One Health-based control strategies. Full article

Domoic acid (DA) is a neurotoxic terpenoid compound produced by certain marine algae. It accumulates through the food web and poses a significant threat to humans and animals by selectively targeting hippocampal neurons, leading to neuronal degeneration, necrosis, and subsequent memory impairment. The primary mechanism involves its potent agonism at glutamate receptors, which induces excessive calcium influx, resulting in excitotoxic cell swelling and death. Recent studies have further elucidated the critical role of downstream oxidative stress and other pathogenic factors in DA-induced neurotoxicity. These insights into its multifaceted mechanism have paved the way for novel therapeutic strategies, highlighting promising directions for future treatment development. Full article

Antimicrobial resistance (AMR) represents a significant global concern, undermining the efficacy of treatments in both human and veterinary medicine. Livestock production plays a major role in the emergence and dissemination of AMR, primarily due to the extensive use of antibiotics for therapeutic, prophylactic, and metaphylactic purposes. Addressing this multifaceted issue necessitates a One Health approach. At the international level, regulatory frameworks are predominantly non-binding, relying on soft-law instruments developed by the World Health Organization (WHO), the Food and Agriculture Organization (FAO), and the World Organization for Animal Health (WOAH, formerly OIE), which advocate for harmonized guidelines and national action plans. In contrast, the European Union has implemented binding regulations, including Regulation (EU) 2019/6 and Regulation (EU) 2019/4, which restrict non-essential antimicrobial use (AMU) and reinforce veterinary accountability. Initiatives such as the Farm to Fork Strategy and platforms like ClassyFarm further advance antimicrobial stewardship by integrating animal welfare, sustainability, and access to EU funding. Achieving substantial reductions in AMR within livestock systems requires coordinated, cross-disciplinary, and multi-level governance efforts. The EU model illustrates how enforceable legal frameworks, combined with science-based monitoring and welfare incentives, can facilitate prudent antibiotic use and promote sustainable animal production. This review aims to provide an integrated overview of international and European strategies for regulating antibiotic use in food-producing animals, focusing on how scientific, veterinary and legal perspectives contribute to combating AMR and promoting animal welfare by emphasizing prevention, and a prudent and responsible AMU. Full article

The production of fats and oils represents a task that is in demand in a variety of industries, including the food industry. Presently, the predominant method of acquiring them is through the processing of plant and animal products, a process that substantially increases the cost of production at all stages. Oleaginous yeasts can serve as an alternative source for obtaining edible oils, as demonstrated by yeast strains employed in the development of palm oil analogues. In this study, we created and characterized a collection of oil-producing yeast species obtained from various natural sources. These species were identified using MALDI-TOF and Sanger sequencing. The isolates were qualitatively and quantitatively tested for their ability to grow on various culture media compositions. The oil-producing strains were characterized by their fatty acid profile and lipidome composition. In addition, we evaluated the biotechnological potential of these organisms as producers of fatty acid- and fat-related products. As a result, the collection contains 100 strains, 31 of which are oleaginous yeasts, and three strains show potential as promising producers of edible oil analogues. Our research demonstrates the benefits of searching for and studying natural yeast strains, both from a fundamental science perspective and for the creation of future innovative biotechnological solutions in the food industry. Full article

As one of the few carotenoid-producing microorganisms, Rhodotorula mucilaginosa remains underexplored for its antioxidant activity. This study investigated the effects of R. mucilaginosa JAASSRY on D-galactose-induced aging mice. The high-dose JAASSRY (HR) significantly increased body weight by 9.89% compared to the model group (AM), while reducing organ indices of the spleen, liver, kidneys, and brain (p < 0.01). Compared with the AM group, the HR group exhibited increased serum activities of SOD (20.26%), GSH-Px (9.03%), and CAT (133.01%), with a 24.87% decrease in MDA level. In brain tissue, SOD, GSH-Px, and CAT activities increased by 79.49%, 8.45%, and 60.23%, respectively, while MDA decreased by 8.29%. R. mucilaginosa JAASSRY also dose-dependently alleviated structural damage in the hippocampus and spleen and improved motor strength and learning-memory capacity. Furthermore, R. mucilaginosa JAASSRY increased the abundance of Lactobacillus and reduced Proteobacteria, Helicobacter, and Oscillospira, while enhancing antioxidant capacity by modulating nucleotide, lipid, and carbohydrate metabolism. Lactobacillus and Pediococcus were positively correlated with memory latency and CAT/SOD activities (p < 0.05), whereas Actinormyces and Dehalobacterium showed negative correlations. Notably, HR performed comparably or superiorly to β-carotene in improving cerebral oxidative stress and beneficial microbiota, suggesting its potential in neuroprotection and gut–brain axis regulation. In conclusion, R. mucilaginosa JAASSRY mitigates aging-related oxidative damage and behavioral deficits by modulating gut microbiota structure and function, demonstrating its promise as a β-carotene alternative in animal husbandry and functional foods. Full article

The global demand for new ingredients and healthier food products is on the rise. Global challenges like rapid population growth, climate change, and emerging pandemics are putting a strain on food security for future generations. This makes it crucial to seek alternatives for producing nutrient-rich foods using more sustainable methods. In this context, proteins are an essential macronutrient for humanity. Plant-based proteins are becoming increasingly popular for the following reasons: their sustainability, as they have a lower environmental impact compared to animal-based proteins, provided they are consumed locally; their nutritional value, since they contain all the essential nutrients when consumed in a varied way and do not contain limiting amino acids; their potential accessibility; and the health benefits they offer. Consequently, the food industry is developing an increasing market of protein concentrates and isolates from plant sources using wet or dry methods. In particular, dry fractionation is expected to play a key role in enhancing food sustainability, as it allows protein enrichment without the use of water or energy-consuming operations. This review provides a detailed description of the application of dry fractionation method to Andean grains, with quinoa, amaranth, and kañiwa as prominent examples. The narrative review covers the essential primary processing and pretreatments, assesses the properties of the resulting fractions, and discusses their applications and future trends. This work aims to promote the development of innovative and sustainable food solutions. Full article

This study analyzes the establishment of Local Agri-Food Systems (LAFSs) in the triple-border region between the states of Minas Gerais, Rio de Janeiro, and São Paulo, by identifying and mapping potential areas of primary peasant agri-food production. An integrated analysis of data sources was treated, processed, and integrated into a common spatial support. Land use and land cover data were used from demographic and agricultural censuses, from the Rural Environmental Registry, agrarian reform settlement projects and conservation units. Our study revealed that 23.73% of the regional area has potential for peasant production, identifying four regions that stand out in terms of this potential. The area presented livestock and animal husbandry as the main agri-food chain, with potential for processing within the territory itself, in addition to extractive activities in the Atlantic Forest biome. The results indicate that there are possibilities for the establishment of LAFSs as a local development strategy associated with social inclusion and environmental responsibility, although there is a need to expand and strengthen the transportation and marketing channels for products from these short chains. The cartographies produced aim to contribute as auxiliary instruments to land use planning and management, seeking to strengthen LAFSs at different scales of governance. Full article

Organic foods are often more expensive because the use of synthetic pesticides, herbicides, and fertilizers is prohibited, and production generally requires more labor. Consumers may feel that organically produced foods are healthier than conventionally produced; however, studies on nutritional value are mixed. This review examines existing reports of micronutrients, including vitamins, minerals, and other bioactive compounds, in plant- and animal-based foods from organic and conventional systems. A literature review was conducted using keywords related to micronutrients, organic farming, and conventional farming. Because the number of available studies was limited, no publication date restrictions were applied. After a preliminary screening of abstracts to determine their relevance to the study’s purpose, 120 articles were included. Reports are reviewed describing higher amounts of vitamin C, vitamin E, magnesium, iron, and polyphenols in organic produce and of certain animal products showing more antioxidants or fat-soluble vitamins. At the same time, many studies found no noteworthy differences, and some authors report higher nutrient levels in conventionally raised foods. Factors such as soil fertility, fertilizer use, and climate conditions may then influence micronutrient food content as much or more than farming methods. While organic foods may sometimes provide enhanced micronutrient levels, the overall evidence from existing literature does not support a consistent nutritional advantage associated with organic production. Full article

Background: In silico diet design may represent a flexible approach in diet planning and adaptation to a variety of conditions, and it may take advantage from standard diet(s) as reference template(s). The concept of standard diet(s) is, however, quite vague and poorly defined. Objective: The aim of this work was to develop templates of omnivorous (OMN), lacto-ovo-vegetarian (LOV), and vegan (VEG) standard diets, based on data produced in European countries and the USA in 1998–2024, and adapted to an adult subject requiring ≈2200 kcal/day. Design: Online databases were used to identify papers containing experimentally determined (EXP) data of daily food frequencies, or reporting dietary recommendations (REC) from (inter)national agencies or specific studies. Only sources reporting quantitative food data (as g/day) in OMN, LOV, and VEG diets were accepted. Results: Out of >200 publications initially identified, 24 EXP and 20 REC sources complied with the selection criteria. By combining the EXP and REC data within each diet type, total meat intake in OMN diet was 99 ± 36 g/day. Total dairy food in LOV diets (247 ± 107 g/day) tended to be lower (by ≈15%, NS) than in OMN diets (272 ± 100). In VEG diets, total vegetal foods were ≈33% greater than in LOV (p < 0.01), and ≈1-fold greater than in OMN ones (p < 0.00001). Total cereal foods were similar in OMN (272 ± 122) and LOV (264 ± 122) diets, but tended to be ≈20–25% greater in VEG diets (to 326 ± 103, NS). Potato and other starchy foods were not different among the three diets. Legumes and pulses were modestly but insignificantly greater in LOV (55 ± 25) and VEG diets (112 ± 137) than in OMN ones (31 ± 24). Soy products were greater in VEG than in LOV diets. The “nuts, seeds, and spreads” food group in VEG diets was ≈3-fold greater than in OMN (p < 0.0005), and ≈90% greater than in LOV diets (p < 0.002). Fruit intake in VEG diets was ≈14% (p = NS) and ≈ 60% (p < 0.005) greater than in LOV and OMN diets, respectively. Finally, the “protein and energy-rich vegetal alternatives” food group in LOV and VEG diets was ≈5- to ≈6-fold greater than in the OMN diet (p ≤ 0.001). Conclusions: The exclusion of meat, fish, and egg in LOV diets is not compensated by increased dairy foods, rather by more total vegetal foods and protein-rich vegetal alternatives. VEG diets replace animal-derived proteins mainly with nuts, seeds, and spreads, soy products and protein-rich vegetal alternatives. On the basis of these data, templates to design “standard” OMN, LOV, and VEG diets are proposed. Full article