Search Results (1,903)

It is well known that food waste, especially perishable fruits, is one of the pressing issues worldwide, and as much as 50% of harvested fruits are wasted in developing countries as a result of poor preservation methods. Other traditional options such as plastic films or chemical preservatives are harmful to the environment and to our health. In this work, the limitations are overcome through the fabrication of an innovative camellia saponin/sodium alginate (CS/SA) composite hydrogel film that not only recycles agricultural waste but also improves fruit protection. CS/SA films were prepared by ionic crosslinking with CaCl₂ with different CS content (0–10% w/v, corresponding to 0–3.1 wt% in air-dried films). Detailed SEM, FTIR, XRD and rheological studies indicated that CS addition led to a gradual microstructural densification, stronger intermolecular interactions (involving hydrogen bonding and electrostatic complexation) and superior viscoelasticity, with the best performance at 8% CS (2.5 wt% in dried film). Mechanical tests confirmed that the stable CS/SA film showed higher tensile strength (152 kPa) and compressive strength (353 kPa) than pure SA (10 kPa) with a relatively low Young’s modulus (0.82 MPa) and high elongation at break (116.33%), which could be easily peeled off from fruit surfaces—an essential benefit of this over stiff chitosan/alginate composites. Structure: The composite film exhibited lower porosity (103.2%), reduced moisture content (94.7%), a controlled swelling ratio (800%) and improved barrier property with a water vapor permeability of 1.3 $\times {10}^{-6}$ g·m⁻¹·s⁻¹·kPa⁻¹ and an oxygen permeability of 1.9 × cm³$·\mathsf{\mu }$m·m⁻²·d⁻¹·kPa⁻¹. The 8% CS film showed very strong antioxidant activity (86% DPPH scavenging). Results of application tests on bananas and strawberries indicated that the ripening process was delayed by the CS/SA coatings, the decay rate was decreased from 99.9% (uncoated control) to 55.6% after 9 days, the weight loss was reduced to 29.3%, and the fruit’s firmness and titratable acidity were maintained. This degradable, multifunctional hydrogel film has the potential to be a sustainable measure to simultaneously mitigate food waste, valorize agricultural byproducts, and protect the environment, which could offer substantial benefit for enhancing global food security as well as fruit shelf life. Full article

The mosaic brain evolution perspective states that the relative sizes and functions of brain regions adapt to living environments and behavioural motivation. Early life adversity brings changes to brain structure, function, and patterns of cognitive processing of food cues. Specific brain development patterns are associated with subsequent disordered eating, which, on the one hand, increases the risk of obesity and metabolic syndrome, and, on the other hand, leads to mental health problems, such as depression and anxiety. This review intends to synthesise aberrant brain development indices, describe aberrant brain developmental trajectories, summarise aberrant neural markers of cognitive processing of food cues, conclude how early life adversity affects disordered eating through aberrant brain development patterns, and provide neural implications for future disordered eating research and intervention. Full article

Haloarchaea are moderate and extreme halophilic microorganisms inhabiting hypersaline environments characterised by high ionic and oxidative stress due to extremely high salt concentrations and high incidence of UV radiation (mainly in spring and summer). To be alive and metabolically active under these harsh conditions, haloarchaeal strains have developed molecular adaptations, like hyperpigmentation. Among the carotenoids produced by haloarchaeal species, the C₅₀ carotenoid called bacterioruberin (BR) and its derivatives, monoanhydrobacterioruberin and bisanhydrobacterioruberin, are the predominant natural pigments produced. This review aims to highlight the most significant characteristics of BR and their derivatives, as well as a description of the biological activities already reported that could provide benefits for human health, including antitumoral, immunomodulatory, antioxidant, skin protectant, antilipidemic, antiglycemic, and anti-atrophic effects, in addition to showing potential positive effects on sperm cells cryopreservation. Overall, C₅₀ carotenoids are fascinating natural biomolecules that could be utilised in processed food and nutraceuticals or as tools in the context of new strategies and/or pharmaceutical formulations to combat various human diseases or metabolic disorders. Full article

Egg products are a convenient and safe form of eggs, possessing valuable nutritional and functional properties. The egg processing industry is responsible for the enormous amounts of biomass in the form of animal by-products (ABPs). According to EU legislation, the ABPs are under strict control from the formation to the disposal of biomass, as they carry a risk to the ecosystem and public health. For this reason, restrictions have been introduced on their use after disposal, ranging from bioactive applications in medical, cosmetic, and pharmaceutical products, as well as feed. The shells are subject to special conditions for processing and use. The by-products of egg breaking are divided into solid (eggshells and eggshell membranes) and liquid (technical albumen) by-products. The biological value is determined by the composition, which varies significantly across the by-products. In the context of the circular economy, all egg by-products contain valuable substances that can be used in food and non-food industries. First, eggshells are the leading by-product, composing 95% of the inorganic substance calcium carbonate, which, after processing, can be used in agriculture, food and feed industries, and medicine. Second, there is a liquid by-product containing proteins from the egg white and a small part of fats from the yolk. Literature data on this by-product are scarce, but there is information about its use as a feed additive, while the extracted and purified proteins can be useful in pharmacy. Egg membranes constitute only 1% of the egg mass, but humanity has long known about the benefits of collagen, keratin, and glycosaminoglycans, including hyaluronic acid, which compose this material. The processed membranes can be used as a food additive, in cosmetics, medicine, or pharmacy, just like other egg by-products mentioned above. This literature review focuses on the possible methods and techniques for processing by-products and their potential application. The literature sources in this review have been selected according to their scientific and practical applicability. The utilization of these by-products not only reduces the impact on the environment but also facilitates the creation of value-added materials. Full article

Chestnut (Castanea sativa Mill.) is a Mediterranean staple food valued for its cultural heritage, gastronomic identity, nutritional profile, bioactivities, and socio-economic and environmental relevance. This narrative review synthesizes current knowledge on chestnut fruits and by-products, linking ecophysiology and genetic diversity to chemical composition and functionality. It summarizes the nutrient profile (high starch and dietary fiber; gluten-free; B vitamins; essential minerals; and favorable fatty acids) and the diversity of phytochemicals—particularly phenolic acids, flavonoids, and ellagitannins (e.g., castalagin and vescalagin)—that underpin antioxidant, anti-inflammatory, antimicrobial, anti-proliferative, and metabolic effects demonstrated across in vitro, cellular, and in vivo models. We compare conventional and green extraction strategies (e.g., hydroethanolic, ultrasound-/microwave-assisted, and supercritical and subcritical water), highlighting method-dependent yields, composition, and bioactivity, and the valorization of shells, burs, and leaves within circular bioeconomy frameworks. Technological applications span functional foods (gluten-free flours, beverages, and emulsions), nutraceuticals, and cosmetics (skin-protective and regenerative formulations), and active packaging/biopolymers with antioxidant and antimicrobial performance. We discuss sources of variability (cultivar, environment, maturation, and processing) affecting bioactive content and efficacy, and outline future directions. Finally, this review emphasizes the importance of university-facilitated co-creation with companies and consumers—within the framework of Responsible Research and Innovation—as a pathway to strengthen the economic valorization and full utilization of the chestnut value chain, enhancing its societal relevance, sustainability, and health-promoting potential. Full article

Heavy metal contamination in agricultural soils poses serious threats to food safety, ecosystem integrity, and public health. This study investigates the concentrations, ecological risks, and human health impacts of nine heavy metals Cr, Mn, Co, Ni, Cu, Zn, Pb, As, and V in homestead agricultural soils collected from two depths, surface (0–20 cm) and subsurface (21–50 cm), across industrial and non-industrial regions of Bangladesh, using inductively coupled plasma mass spectrometry (ICP-MS). Results revealed that surface soils from industrial areas exhibited the highest metal concentrations in order of Mn > Zn > Cr > Pb > V > Ni > Cu > As > Co. However, maximum As levels were detected in non-industrial areas, suggesting combined influences of local geology, intensive pesticide application, and prolonged irrigation with As-contaminated groundwater. Elevated concentrations in surface soils indicate recent contamination with limited downward migration. Multivariate statistical analyses indicated that industrial and urban activities are the major sources of contamination, whereas Mn remains primarily geogenic, controlled by natural soil forming processes. Contamination factor (CF) and pollution load index (PLI) analyses identified Pb and As as the principal pollutants, with hotspots in Nairadi, Majhipara (Savar), Gazipur sadar, and Chorkhai (Mymensingh). Ecological risk (ER) assessment highlighted As and Pb as the dominant environmental stressors, though overall risk remained low. Human health risk analysis showed that ingestion is the primary exposure pathway, with children being more susceptible than adults. Although the hazard index (HI) values were within the acceptable safety limits, the estimated carcinogenic risks for As and Cr exceeded the USEPA thresholds, indicating potential long term health concerns. Therefore, the cumulative carcinogenic risk (CCR) results demonstrate that As is the primary driver of lifetime carcinogenic risk in homestead soils, followed by Cr, while contributions from other metals are minimal. These findings emphasize the urgent need for continuous monitoring, improved industrial waste management, and targeted mitigation strategies to ensure safe food production, a cleaner environment, and better public health. Full article

Environmental and economic concerns due to the increasing use of fossil-based chemicals, especially fuel, may be alleviated by production of renewable fuels based on plant biomass, in particular, waste. Multistep cascades of enzymatic reactions are being increasingly sought to enhance the effectiveness of sustainable, environment-friendly processes. The biochemical transformation of lignocellulosic biomass and oils into fatty acid esters (“biodiesel”) involves biomass pretreatment, followed by polysaccharide hydrolysis and sugar fermentation to alcohol, either sequentially or simultaneously. Subsequent trans-esterification with waste or non-food-based oils is usually carried out in an organic solvent. Biocatalysis in aqueous emulsion offers significant advantages. This study presents a novel “one-pot” emulsion-based process for transforming unmodified cellulose and castor oil into biodiesel via hybridized yeasts with cellulose-coated micro-particles incorporating cellulolytic enzymes and lipases. The resultant consolidated bioprocess of saccharification, fermentation, and transesterification (cSFT) promotes effective substrate channeling and can potentially serve as a model for emulsion-based “one-pot” transformations of cellulose into valuable chemicals. Full article

The concept of using ‘acid-adapted’ challenge cultures in the microbial validation of food processes that incorporate an acidic treatment is that they would be more resistant to acid and require a robust process to obtain targeted log reductions. The recent confirmation that acid-adapted Salmonella challenge cultures for droëwors and biltong processes are more sensitive to those processes than non-adapted cultures changes that preference for the use of non-adapted cultures for validation studies with these specific processes. However, it is difficult to achieve > 5-log reductions with non-adapted cultures, one of two USDA-FSIS parameters available for validation of processes that are not aligned with traditional process conditions for dried beef products in the USA (i.e., beef jerky). A natural multipurpose (flavor, antimicrobial) commercial product, described as a refined liquid smoke flavorant, provided >7-log reductions with droëwors when challenged with non-adapted cultures of Salmonella (5-serovar mixture), Shiga toxigenic Escherichia coli (STEC, four-strain mixture), and Listeria monocytogenes (four-strain mixture) as well as a >7-log reduction with biltong processing (vs Salmonella). Comparisons between standard droëwors and biltong processes (all <5-log reductions) using non-adapted challenge cultures vs. the same formulation plus 0.75% pyrolyzed liquid smoke extracts (Flavoset) showed greater and significant (p < 0.05) reductions in duplicate trials with triplicate samples at each sampling point in each trial (total n = 6) when analyzed by repeated measures analysis of variance (RM-ANOVA). Although sold as a flavorant, this study examines the antimicrobial properties of Flavoset 5400L to improve the safety of droëwors and biltong by achieving a >5-log reduction with non-adapted pathogenic challenge cultures. Validation processes for droëwors and biltong established with these parameters should result in greater safety of marinaded, non-thermally processed meats from traditional foodborne pathogens commonly associated with meats or meat processing environments. Full article

The growing prevalence of emerging contaminants in the aquatic environment poses a critical challenge to global water security. Conventional treatment methods often fail to remove persistent pollutants, necessitating the development of sustainable and efficient alternatives. Heterogeneous photocatalysis, one of the advanced oxidation processes, driven by the light-induced generation of reactive species, has emerged as a promising solution. However, the high cost and environmental footprint of conventional photocatalysts limit large-scale implementation. This review explores the innovative use of waste-derived materials, originating from agricultural residues, industrial by-products, food waste, and electronic waste, as green precursors for photocatalyst synthesis. These materials offer unique structural and chemical properties, including a high surface area, tunable bandgaps, and enhanced stability, while supporting circular economy principles. Recent advances in synthesis strategies, performance optimization, and hybrid system integration are critically analyzed, alongside challenges related to scalability, environmental safety, and process standardization. Waste-to-resource approaches in photocatalysis represent a transformative pathway toward sustainable water treatment and resource recovery. Full article

Microbial biofilms in food processing environments pose significant challenges due to their exceptional resistance to conventional sanitation methods, presenting substantial risks to food safety and public health. This review systematically evaluates recent advances in understanding biofilm development across key stages, i.e., initial microbial adhesion, extracellular polymeric substance production, biofilm maturation including resistant phenotypes such as persister cells, and dispersion. Particular emphasis is placed on the molecular mechanisms underlying biofilm formation and the regulatory roles of cyclic-di-GMP and quorum sensing. Crucially, we highlight emerging targeted interventions including enzyme-mediated extracellular polymeric substance disruption, microenvironmental manipulation, quorum sensing inhibitors, metabolic reactivation of persisters (“wake-and-kill”), and controlled biofilm dispersion techniques, clearly outlining their practical applicability and potential limitations in real-world food industry contexts. Moreover, this review uniquely integrates innovative technological developments such as responsive antimicrobial coatings, real-time biosensors, predictive modeling systems, and precision biotechnology approaches. Uniquely, this review integrates molecular mechanisms with practical, stage-specific sanitation strategies and provides actionable insights that can enhance biofilm control, contributing to safer food production practices and im-proved public health outcomes. Full article

Background: Antimicrobial resistance (AMR) is a growing public health concern affecting both medicine and food safety. While Listeria monocytogenes is the primary pathogen of concern, Listeria innocua—commonly found in food and food-processing environments—may serve as a reservoir for resistance genes and a useful indicator of species for surveillance. This study aimed to assess the phenotypic antibiotic susceptibility and detect resistance-associated genes in L. innocua isolates from meat products and processing environments in Poland. Methods: A total of 51 L. innocua isolates were analyzed, originating from raw and processed meat products as well as meat-processing environments. Antimicrobial susceptibility was determined using the disc diffusion method against 18 antibiotics representing multiple classes. Phenotypic resistance was interpreted following CLSI guidelines (CLSI, 2020). Isolates exhibiting resistance or intermediate resistance were further screened for resistance-associated genes using PCR. Results: All isolates were fully susceptible to ampicillin, benzylpenicillin, chloramphenicol, gentamicin, rifampin, trimethoprim-sulfamethoxazole, and vancomycin. High susceptibility was observed for ciprofloxacin, erythromycin, meropenem, trimethoprim, and nitrofurantoin, with only sporadic intermediate responses. Moderate resistance levels were noted for streptomycin (10%) and tetracycline (12%). The lowest susceptibility was recorded for clindamycin and linezolid, with most isolates exhibiting intermediate or resistant phenotypes. Universal resistance to cefotaxime and oxacillin was found. Eighteen distinct resistance patterns were identified. PCR confirmed the presence of several resistance-associated genes, including mecA, lnuA, lnuB, cfr, optrA, and poxtA, consistent with observed phenotypes. Conclusions: This study provides the first detailed characterization of AMR in L. innocua from Polish meat and processing environments. The findings highlight its heterogeneous resistance profiles and potential role as a reservoir of clinically relevant resistance genes. Incorporating L. innocua into surveillance programs may strengthen early detection of emerging resistance and enhance food safety monitoring. Full article

The use of smartphones and digital cameras as color measurement tools is reported. Initially, a careful mathematical analysis of the intrinsic limitations of using an 8-bit RGB color space was conducted, determining the ΔE in terms of sensitivity and conversion error to the CIELab space. Metal coatings were subsequently analyzed under extremely different lighting conditions, obtaining equally different colors. The use of a colorimetric reference card, captured alongside the samples, enabled the minimization of these differences. The possibility of obtaining quantitative results using portable and widely available devices, such as smartphones, even in outdoor environments with uncontrollable lighting conditions provides a valuable analytical tool across various fields, including industrial, decorative, medical, and food applications, especially in the characterization of coatings. Eight-bit RGB devices limit sensitivity in the worst case to ∆E = 1.5. ∆E > 20, as measured by spectrophotometer and smartphone, which was reduced to ∆E < 5 after the proposed processing. Full article

Climate change has contributed to increased mycotoxin contamination in food systems, posing a growing threat to human health, including reproductive health. Our study aimed to investigate how mycotoxins entering the follicular fluid affect oxidative stress processes. We analyzed 88 follicular fluid samples from infertile patients for common mycotoxins, including deoxynivalenol (DON), zearalenone (ZEN), its main metabolite alpha-zearalenol (aZOL), and aflatoxin M1 (AfM1), and examined their relationship with oxidative stress markers (MDA, SOD, GPx, CAT, and TAOC) and hormones (cortisol, estradiol, and anti-Müllerian hormone). Higher mycotoxin levels were associated with increased oxidative stress, particularly elevated MDA levels, and disrupted antioxidant enzyme activity. Notably, DON showed a positive correlation with SOD and estradiol levels, indicating a compensatory antioxidant response, while AfM1 served as a negative predictor. The metabolite aZOL was strongly linked to cortisol, with effects influenced by estradiol levels, implying endocrine-disrupting activity. Importantly, the interaction between DON and AMH appeared to impact dominant follicle development, suggesting a potential mechanism by which environmental toxins impair fertility without directly reducing oocyte or embryo counts. These results highlight the complex, dose-dependent effects of mycotoxins on oxidative and hormonal balances within the follicular environment, with implications for oocyte quality and reproductive success. Better understanding these mechanisms could help develop early diagnostic markers and targeted interventions to improve fertility outcomes in women exposed to changing environmental conditions. Full article

The authors frame greenhouse operation as a Controlled Environment Agriculture (CEA) challenge involving multiple interdependent targets: air temperature and humidity, CO₂ enrichment, photoperiod-constrained lighting, and irrigation under dynamic and limited energy availability. We propose a knowledge-driven, multi-objective Model Predictive Controller whose cost function integrates expert priorities elicited via an online Analytic Hierarchy Process (AHP) survey; these AHP-derived weights parameterize the controller’s objectives and are solved over two 72 h seasonal episodes, so the MPC can anticipate renewable availability and coordinate HVAC, (de)humidification, CO₂ dosing, LED lighting, and irrigation alongside dispatch from photovoltaic and wind sources, battery storage, and the grid. By embedding the physical interdependence of climate variables directly into the decision layer, the controller schedules energy-intensive actions around renewable peaks and avoids counterproductive actuator conflicts. Seasonal case studies (summer/high solar and winter/low solar) demonstrate robust performance: temperature tracking errors of SMAPE 2.25%/3.05% and CO₂ SMAPE 3.72–3.92%; humidity control with SMAPE 7.04–8.56%; lighting and irrigation following setpoints with low NRMSE (0.08–0.14). Summer energy was 59% renewable; winter was only 13%, increasing grid reliance to 77.5% (peaks: 4.57 kW/6.92 kW for 197.7/181.5 kWh). Under water or energy scarcity, the controller degrades gracefully, protecting high-priority agronomic variables while allowing bounded relaxation on lower-priority targets. This expert-informed, predictive, and resource-aware orchestration offers a scalable route to precision greenhouse control within the food–water–energy nexus. Full article

Chitosan (chit) is a specific polymer, an oligosaccharide, that demonstrates several important properties alone or in combination with other compounds or elements. These important properties include being biocompatible with the human body and simultaneously effective in destroying bacteria. Moreover, it is a smart polymer, as it can change its properties when the pH value decreases below about 7. Therefore, chitosan is widely applied in dermo-cosmetics, but it is also intensively investigated for its use in food preservation and the targeted regeneration of teeth in dentistry. Due to these advantageous characteristics, chitosan has been employed in numerous coating systems for biomedical applications. These coatings can be fabricated through a wide variety of procedures involving different deposition techniques, process parameters, and the chemical characteristics of the environment, solution, air or vacuum, as well as the substrate. Chitosan–metallic coatings have often been investigated; however, the use of elementary metals in the form of nanoparticles (NPs) was rarely reported. The main part of this paper is focused on the presentation of chitosan–metallic NPs, in particular, an application of potentially antibacterial noble and semi-noble metals such as Au, Ag, Cu, and Zn, intensively investigated by the author. The deposition methods and their limitations, the differences in properties of such coatings and those possessing Ag, Sr, Zn, and other metals in the form of cations in chemical compounds, and the perspectives of chit–MeNPs (metal nanoparticles) are thoroughly considered, and future research is proposed. Full article