Search Results (2,990)

As a common substitute for brominated flame retardants (BFRs), organophosphate flame retardants (OPFRs) have been insufficiently studied in terms of their ecotoxicological impacts on plastic biodegradation processes in invertebrate systems. This study investigated the impact of an OPFR, tris (1-chloro-2-propyl) phosphate (TCPP), on the dietary behavior and gut microbiota of Galleria mellonella (Linnaeus, 1758) (Lepidoptera: Pyralidae) larvae during the biodegradation of rigid polyurethane (RPU), as well as the fate of TCPP. The results show that TCPP interfered with larval feeding activity, hindered the nutritional conversion of food, and triggered metabolic compensation through lipid reserve catabolism. Notably, mass balance analysis revealed that bioaccumulation of TCPP was negligible, with most of it excreted through frass, indicating limited biodegradation of this organophosphate ester. 16S rRNA sequencing indicated that TCPP drove the reconstruction of gut microbiota in larvae and identified three dominant bacteria of Morganellaceae, Enterobacteriaccae, and Staphylococcaceae families, as well as non-dominant bacteria of Klebsiella and Vagococcaceae families, as characteristic microbiota contributing to RPU and TCPP biotransformation. This study serves as a reminder to pay attention to the toxicity, migration, and transformation of OPFRs in biodegradable plastics. Notably, TCPP, a dominant chlorinated OPFR, exhibits environmental persistence with limited biodegradability and low bioaccumulation, traits which hinder the spontaneous attenuation of plastic waste in ecosystems and undermine the sustainability of the plastic lifecycle. This work emphasizes the need to integrate risk assessments of specific additives into the plastic waste management framework and to develop targeted detoxification strategies for promoting a sustainable material lifecycle. Full article

There is currently no routine systematic assessment of welfare on Iranian dairy farms and no industry-recognised welfare assessment protocols. Therefore, we aimed to design a comprehensive welfare assessment protocol and use it to assess dairy cattle welfare on Iranian dairy farms that could serve as baseline data. Out of the 54 farms on which milking time was measured, 14 had cows spending ≥4 h a day for milking. Additionally, 17/43 farms provided <6 cm of water trough length per cow, and 9/46 farms provided <47 cm of feed trough length per cow, falling short of international guidelines. Hoof trimming was considered a routine procedure with 51/56 farms trimming the hooves ≥2 times a year. The main housing problem in bedded-pack farms was lack of resting areas with 18/29 farms providing less space than the minimum requirement of 5.4 m²/cow, while in free-stall farms bedding depth was the principal housing issue with 16/28 providing ≤10 cm of bedding. Finally, only 31/42 farms that provided farm data kept a record of more than half of the parameters that we asked for. Our findings indicate that a high proportion of farms did not provide sufficient resources or implement management practices necessary to meet welfare requirements of dairy cattle on Iranian farms. Full article

Climate change is causing an increase in global temperatures, with significant impacts on dairy production. This systematic review analyzes the challenges of new climate scenarios, focusing on the resilience and adaptation strategies of dairy systems. The PRISMA methodology guided the review process using the Scopus and Web of Science databases. After applying exclusion criteria, 30 articles published between 2015 and 2025 were selected. The studies included analyses of the effects of heat stress on animal parameters (I), socioeconomic parameters (II), and technological adaptation tools (III) in various geographic regions. Most research over the last decade addresses category (I), with a greater concentration in the Northern Hemisphere. The Temperature Humidity Index (THI) emerged as the main indicator of heat stress, associated with the physiological responses of dairy cattle. Heat stress reduces milk production, feed intake, reproductive performance, and overall animal health, often leading to increased slaughter rates. Adaptation strategies include genetic selection for heat tolerance, improved environmental monitoring, cooling technologies, and optimized nutritional and management practices, applicable to both pasture and feedlot systems. Despite being among the main producers, studies on the topic in South American countries are still scarce in the literature. Full article

Calcium propionate serves as an effective feed additive for preventing hypocalcemia and ketosis in early-lactation dairy cows. This study investigated the effects of dietary calcium propionate levels (0, 200, 350, and 500 g/d) on productive performance, milk minerals, and fatty acid profiles in 32 Holstein cows. Productive performance was monitored daily through the peak of lactation (36–125 days in milk, DIM), with milk samples collected on 7, 21, and 35 DIM for mineral and fatty acid analysis. Results showed that calcium propionate supplementation tended to increase peak lactation milk yield, with the highest value (53.67 kg/d) observed in the 350 g/d group. The milk K concentration exhibited a quadratic increased response, with greater values in the 200 g/d and 350 g/d groups. Conversely, milk Mg and P concentrations and the proportions of short-chain fatty acids (C6:0, C8:0, and C12:0) decreased linearly with increasing calcium propionate supplementation levels. Furthermore, the proportions of C18:2 cis-9,12 and the sum of polyunsaturated fatty acids exhibited a quadratic decreased response, being lowest in the 350 g/d group. In conclusion, supplementing 350 g/d of calcium propionate optimally improved lactation performance and altered milk mineral and fatty acid profiles, suggesting its value in early-lactation management. Full article

To provide a scientific basis for pest control, this study evaluated the insecticidal activity of novaluron against the fall armyworm (Spodoptera frugiperda) and diamondback moth (Plutella xylostella). The leaf-dip method determined the toxicity of novaluron to second-instar larvae, while corn leaves and cabbage treated with sublethal (LC₁₀) and median lethal concentrations (LC₅₀) of novaluron were used to feed the larvae. Enzyme-linked immunosorbent assays (ELISA) measured the activities of detoxifying enzymes [carboxylesterase (CarE), cytochrome P450 (P450), glutathione S-transferase (GST), and acetylcholinesterase (AChE)] and ecdysteroid (Ecd) levels in the pests after 24 and 48 h of treatment. Results indicated that after 24 h, AChE was involved in diamondback moth metabolism and CarE activity was inhibited. After 48 h, P450 and GST participated in fall armyworm detoxification, whereas P450 and GST were active in diamondback moth detoxification, with other enzyme activities normalizing. Novaluron also altered Ecd levels in both pests. These results demonstrate differing detoxification mechanisms in fall armyworm and diamondback moth, likely due to their unique physiological and ecological traits, and support the potential use of novaluron in pest management strategies. Full article

Campylobacter is a leading cause of human gastroenteritis, with poultry serving as the primary reservoir host. Effective preharvest control strategies are crucial for preventing or reducing Campylobacter contamination on meat surfaces. As concerns grow regarding the use of antimicrobials in animal agriculture, the importance of non-antimicrobial preharvest strategies in poultry production has become increasingly significant. This comprehensive review focuses on the biology of Campylobacter, its impact on public health, and current and emerging preharvest strategies, with a special emphasis on vaccination. Preharvest strategies are broadly classified into biosecurity measures, gut microbiota modifications using prebiotics, probiotics, postbiotics, feed additives, and vaccination. While some vaccines have proven to be effective in research settings, no commercial vaccines are currently available. Because no single strategy can effectively combat Campylobacter, integrating multiple approaches, such as improved biosecurity measures, immunization, and dietary modifications, may provide a solution for reducing Campylobacter loads in poultry. Embracing a “One Health” approach, gaining a deeper understanding of Campylobacter pathophysiology, advancing vaccine technology, and implementing holistic farm management practices will be essential for the sustainable control of Campylobacter and for reducing the risk of human campylobacteriosis. Full article

This review examines the diverse ways in which probiotics, defined as live microorganisms that provide health benefits to the host when administered in adequate amounts, contribute to animal health and welfare across both livestock and companion species. By modulating gut microbiota, enhancing immune responses, and suppressing harmful pathogens, probiotics represent an effective strategy for disease prevention and performance improvement without reliance on antibiotics. In livestock production, these beneficial microbes have been shown to optimize feed utilization, support growth, and reduce methane emissions, thereby contributing to more sustainable farming practices. Their role extends beyond productivity, as probiotics also help mitigate antimicrobial resistance (AMR) by offering natural alternatives to conventional treatments. In aquaculture, they further promote environmental sustainability by improving water quality and reducing pathogen loads. For companion animals such as dogs and cats, probiotics are increasingly recognized for their ability to support gastrointestinal balance, alleviate stress through gut–brain axis interactions, and aid in the management of common conditions including diarrhea, food sensitivities, and allergies. The integration of probiotics into veterinary practice thus reflects a growing emphasis on holistic and preventive approaches to animal health. Despite these advances, several challenges remain, including variability in strain-specific efficacy, regulatory limitations, and cost-effectiveness in large-scale applications. Emerging research into precision probiotics, host–microbiome interactions, and innovative delivery methods offers promising avenues to overcome these barriers. As such, probiotics can be regarded not only as functional supplements but also as transformative tools that intersect animal health, productivity, and sustainability. Full article

The diverse phytochemical profiles of host plants can significantly influence their interactions with herbivores and natural enemies. This study investigated the ‘bottom-up’ effects of several bell pepper and eggplant cultivars on the development, reproduction, and survival of the green peach aphid, Myzus persicae (Sulzer) (Hemiptera: Aphididae), and its predators, Aphidoletes aphidimyza (Rondani) (Diptera: Cecidomyiidae) and Chrysoperla carnea (Stephens) (Neuroptera: Chrysopidae). We analyzed the leaves of each cultivar for levels of total flavonoids, phenols, anthocyanins, and key defensive enzymes. The eggplant cultivar ‘Longo’ exhibited the highest concentration of secondary metabolites. Aphid populations reared on this cultivar’s leaves showed a slower growth rate compared to those on other cultivars. Conversely, predators fed on these aphids demonstrated higher rates of population growth and produced more offspring. Accordingly, the intrinsic rate of natural increase (r) was lower for aphids feeding on ‘Longo’, but significantly higher for both A. aphidimyza and C. carnea when fed those aphids. These results demonstrate that elevated secondary metabolites on ‘Longo’ suppress the performance of M. persicae while enhancing predator efficiency, thereby providing a phytochemical-based approach that can serve as an effective component of integrated pest management (IPM) programs. Full article

Rice is a major dietary component for more than half of the world’s population, and its cultivation requires a careful balance of nutrients to ensure high yields and sustainable practices. Soil-derived N₂O fluxes represent a major environmental challenge with global implications. While agriculture is a necessary activity to feed a growing population, it must evolve to minimize its ecological footprint. This review provides an update on the effects of nitrogen fertilizer application, such as ammonium nitrate (NH₄NO₃), urea (CO(NH₂)₂), ammonium sulfate ((NH₄)₂SO₄), and calcium ammonium nitrate (CAN), on N₂O emissions from rice cultivation. The role of various nitrification inhibitors (e.g., dicyandiamide (DCD), 2-chloro-6-(trichloromethyl) pyridine (nitrapyrin) and 3,4-dimethylpyrazole phosphate (DMPP)) in minimizing the release of N₂O from soils to the atmosphere was also discussed. Here, we described N₂O production by nitrification and denitrification processes in the paddy rice field, and then summarized the strategies, such as optimized fertilizer use, improved drainage and water management, and the use of organic amendments, that can enhance crop productivity while promoting sustainable reductions in N₂O emissions. Full article

Waterlogging (WL) is an abiotic stress that severely limits crop yield. However, limited research has addressed the effects of long-term WL stress at different developmental stages on the yield and physiological responses of forage maize. In this study, forage maize plants were subjected to 14-day WL stress at the emergence (E), four-leaf (V4), eleven-leaf (V11), and tasseling (VT) stages. Plant height significantly decreased by 60% at the E stage and 48% at the V4 stage when exposed to 14-day WL. Leaf area decreased by 79% at the E stage, and the number of green leaves decreased most significantly at the VT stage. Chlorophyll fluorescence (Fv/Fm) and the relative chlorophyll content index (RCI) decreased most significantly at the V4 stage. The lysigenous aerenchyma formation rate of the roots increased significantly after 14-day WL at the V4 stage, whereas the number of adventitious roots increased most significantly at the V11 stage. The hydrogen peroxide (H₂O₂) and malondialdehyde (MDA) contents, which are indicative of the root oxidation state, exhibited the highest increase at the E stage. In addition, at the E and V4 stages, the expression of genes related to energy metabolism and lysigenous aerenchyma formation in the roots was upregulated after 14-day WL. The total dry matter (DM) of maize after harvest decreased most significantly when exposed to 14-day WL at the V4 stage, while acid detergent fiber (ADF) and neutral detergent fiber (NDF) increased with the developmental stages. Consequently, total digestible nutrients (TDNs) and the relative feed value (RFV) decreased with advancing developmental stages, with the highest decrease at the VT stage. These results demonstrate that effective drainage management during the early developmental stage (V4) is more important to prevent forage maize yield loss due to prolonged WL stress, which is expected to increase in frequency due to climate change, and management during the later developmental stage (VT) is critical to prevent decreases in feed values. These findings provide valuable insights into the physiological responses of forage maize to WL stress. Full article

Background: Schizophrenia (SCZ) is a psychiatric disorder that negatively impacts patients’ quality of life, frequently inducing difficulties in managing day-to-day tasks. Current research is persistently working on finding therapeutic methods to alleviate the positive and negative symptoms, as well as the associated cognitive dysfunctions. Since the main therapeutic approach in SCZ is antipsychotics, the current study aimed to explore the effects of typical (haloperidol, HAL) vs. atypical (risperidone, RIS) antipsychotics on the cognitive functions in an animal model (Danio rerio) of SCZ, obtained by ketamine (KET) administration. Methods: The cognitive evaluation of the zebrafish was performed using memory and learning tests based on two stimuli: food and colours (i.e., T memory test and novel object recognition (NOR) test, respectively). Results: According to the behavioural analyses, HAL significantly enhanced the cognitive performances of the SCZ model, as compared to RIS. Nonetheless, HAL and RIS exhibited comparable effects on social behaviour in the SCZ model. Interestingly, both HAL and RIS enhanced the interest for the novel object in the NOR test in control individuals, but significantly decreased it in the SCZ model. The interaction between KET and RIS could exhibit sedative properties. Conclusions: Both typical (HAL) and atypical (RIS) antipsychotics alleviated cognitive, socio-affective, and decision-making impairments in a ketamine-based adult zebrafish model of schizophrenia. HAL was more effective, particularly in food-stimulated decision-making compared to novel object or social stimuli. Colour influenced behavioural responses, with silver linked to prey/feeding effects and red perceived as aversive. The KET–RIS combination induced exploratory impairments, possibly due to sedative effects. These findings highlight differential pharmacological and ethological modulation of schizophrenia-like behaviours. Full article

In modern livestock production, obtaining accurate body weight measurements for pigs is essential for feeding management and economic assessment, yet conventional weighing is laborious and can stress animals. To address these limitations, we developed a contactless image-based pipeline that first uses BiRefNet for high-precision background removal and YOLOv11-seg to extract the pig dorsal mask from top-view RGB images; from these masks we designed and extracted 17 representative phenotypic features (for example, dorsal area, convex hull area, major/minor axes, curvature metrics and Hu moments) and included camera height as a calibration input. We then compared eight machine-learning and deep-learning regressors to map features to body weight. The segmentation pipeline achieved mAP₅₀–₉₅ = 0.995 on the validation set, and the XGBoost regressor gave the best test performance (MAE = 3.9350 kg, RMSE = 5.2372 kg, R² = 0.9814). These results indicate the method provides accurate, low-cost and computationally efficient weight prediction from simple RGB images, supporting frequent, noninvasive monitoring and practical deployment in smart-farming settings. Full article

Despite the oligotrophic conditions of the southeastern Levantine Sea and northern Red Sea, six lobster species—five slipper lobsters (Scyllaridae) and one spiny lobster (Palinuridae)—maintain permanent, reproducing populations in the study area. Additionally, there are isolated records of four other sporadic lobster species. In the southeastern Mediterranean, permanent species include the Mediterranean slipper lobster,Scyllarides latus, small European locust lobster, Scyllarus arctus, and pygmy locust lobster, Scyllarus pygmaeus. In the northern Red Sea, they include the clamkiller slipper lobster, Scyllarides tridacnophaga, Lewinsohn locust slipper lobster, Eduarctus lewinsohni, and pronghorn spiny lobster, Panulirus penicillatus. This review synthesizes current knowledge of their biology and ecology, including distribution, habitat, reproduction and development, feeding, predators and anti-predatory adaptations, behavior, sensory modalities, environmental impacts, threats, and conservation. Recent advances focus mainly on larger, commercially valuable species (S. latus, S. tridacnophaga, P. penicillatus), while major gaps remain for oceanic post-embryonic stages and the nektonic nisto postlarva, as well as for smaller, often cryptic species (S. arctus, S. pygmaeus, E. lewinsohni). Addressing these gaps will require targeted research, using modern methodologies, in coastal, deep, and open waters, coupled with citizen-science surveys. While many Indo-Pacific decapods have been established in the Mediterranean, no immigrant lobster species have successfully colonized Levant waters, despite rare records of three non-indigenous species (NIS). However potential NIS predators and shifts in mollusk compositions, the main prey of some native lobsters, may affect the latter. Large lobsters remain targeted by fisheries despite protective regulations, which are not always effective or obeyed. No-take marine protected areas (MPAs) or nature reserves can be effective if sufficiently large and well-managed. Habitat loss from marine construction can be partly compensated by stable, environmentally safe artificial reefs tailored to lobster behavioral ecology. The categories of the studied lobsters’ species in the International Union for Conservation of Nature (IUCN) Red List of Threatened Species, last updated over fifteen years ago, should be re-evaluated. Full article

Background: Antimicrobial drugs are used to treat bacterial diseases in livestock production systems, including bovine respiratory disease (BRD) in feedlot cattle. It is recommended that therapeutic antimicrobial use (AMU) in food animals be informed by diagnostic tests to limit the emergence of antimicrobial resistance (AMR) and preserve the effectiveness of available drugs. Recent evidence demonstrates preliminary support for the pen as a prospective target for AMR testing-based interventions in higher-risk cattle. Methods: A previously reported agent-based model (ABM) was modified and then used in this study to investigate the potential for different pen-level sampling and laboratory testing-informed BRD treatment strategies to favorably impact selected antimicrobial stewardship and management outcomes in the western Canadian context. The incorporation of sample testing to guide treatment choice was hypothesized to reduce BRD relapses, subsequent AMU treatments and resultant AMR in sentinel pathogen Mannheimia haemolytica. The ABM was extended to include a discrete event simulation (DES) workflow that models the testing process, including the time at sample collection (0 or 13 days on feed) and the type of AMR diagnostic test (antimicrobial susceptibility testing or long-read metagenomic sequencing). Candidate testing scenarios were simulated for both a test-only control and testing-informed treatment (TI) setting (n = 52 total experiments). Key model outputs were generated for both the pen and feedlot levels and extracted to data repositories. Results: There was no effect of the TI strategy on the stewardship or economic outcomes of interest under baseline ecological and treatment conditions. Changes in the type and number of uses by antimicrobial class were observed when baseline AMR in M. haemolytica was assumed to be higher at feedlot arrival, but there was no corresponding impact on subsequent resistance or morbidity measures. The impacts of sample timing and diagnostic test accuracy on AMR test positivity and other outputs were subsequently explored with a theoretical “extreme” BRD treatment protocol that maximized selection pressure for AMR. Conclusions: The successful implementation of a pen-level sampling and diagnostic strategy would be critically dependent on many interrelated factors, including the BRD treatment protocol, the prevalences of resistance to the treatment classes, the accuracy of available AMR diagnostic tests, and the selected “treatment change” thresholds. This study demonstrates how the hybrid ABM-DES model can be used for future experimentation with interventions proposed to limit AMR risk in the context of BRD management. Full article

Given the climate change observed in the past few decades, sustainable development and the use of renewable energy sources are urgent. In this scenario, hydrogen production through electrolyzers is a promising renewable source and energy vector because of its ultralow greenhouse emissions and high energy content. Hydrogen can be used in a variety of applications, from transportation to electricity generation, contributing to the diversification of the energy matrix. In this context, this paper presents an autonomous isolated DC microgrid system for generating and storing electrical energy to be exclusively used for feeding an electrolyzer hydrogen production plant, which has been retrofitted for green hydrogen production. Experimental verification was performed at Itaipu Parquetec, which consists of an alkaline electrolysis unit directly integrated with a battery energy storage system and renewable sources (e.g., photovoltaic and wind) by using an isolated DC microgrid concept based on DC/DC and AC/DC converters. Experimental results revealed that the new electrolyzer DC microgrid increases the system’s overall efficiency in comparison to the legacy thyristor-based power supply system by 26%, and it autonomously controls the energy supply to the electrolyzer under optimized conditions with an extremely low output current ripple. Another advantage of the proposed DC microgrid is its ability to properly manage the startup and shutdown process of the electrolyzer plant under power generation outages. This paper is the result of activities carried out under the R&D project of ANEEL program No. PD-10381-0221/2021, entitled “Multiport DC-DC Converter and IoT System for Intelligent Energy Management”, which was conducted in partnership with CTG-Brazil. Full article