Search Results (72)

Achieving sustainability in rural cold chain logistics requires resolving inherent conflicts among participating agents. This paper develops an evolutionary game theory framework to examine the dynamic interactions between government regulators, cold chain enterprises, and agricultural producers. The model identifies three evolutionarily stable strategies (ESS) under different policy environments. Numerical simulations, using parameters calibrated from industry data and survey results, quantify the impact of key policy variables: (1) Subsidy intensity has a diminishing marginal effect on green technology adoption, with an optimal range between 12–18% of project cost; (2) Monitoring probability exhibits a threshold effect, needing to exceed 60% to deter non-compliance effectively; (3) Farmer organization reduces system stabilization time by approximately 30%. Our results challenge the conventional focus on single-policy solutions and instead demonstrate the necessity of integrated approaches that simultaneously address economic viability, operational efficiency, and community engagement. These insights offer evidence-based guidance for designing multi-stakeholder governance mechanisms in resource-constrained rural settings. Full article

Dengue virus (DENV) is a mosquito-borne RNA virus that causes serious illness in humans, ranging from mild fever to severe clinical manifestations, with dengue virus type 2 (DENV-2) being the most virulent among its four serotypes. Despite extensive research, no specific antiviral therapy is currently available, making the host-directed method an appealing therapeutic approach. Evidence shows that DENV manipulates host kinase-driven phosphorylation pathways to control viral pathogenesis. Using the kinase–substrate phosphomotif approach, we predicted phosphorylation sites across the DENV proteome and their potential human kinases. The predicted kinase–substrate interactions were systematically integrated with DENV-2-induced human phosphoproteome datasets, protein–protein interactions, and experimentally-validated viral phosphosites. The therapeutic relevance of the identified host kinases was corroborated by the impact of their inhibitors on DENV-2 infection. Among the 359 potential human kinases predicted to phosphorylate DENV-2 proteins, based on human phosphoproteome and kinase–viral protein interaction analyses, CDK9 emerged as a central hub kinase. Molecular docking analyses further revealed that the host kinases CDK9, EEF2K, HASPIN, and TNNI3K form stable interactions with the viral capsid and NS5 proteins. Additionally, a conservation analysis suggested that the predicted phosphorylation sites are evolutionarily conserved across DENV-2 strains. Computational prediction tools supported the predicted kinase–substrate interactions, underscoring the role of host kinases as key regulators of DENV infection, which may act as potential therapeutic targets. This study highlights the interplay between dengue viral and host proteins, providing insights into host-directed therapeutic strategies for DENV-2 infection and their potential to address the current lack of effective antiviral interventions. Full article

Driven by the “dual carbon” goal and the strategy for cultivating new productive forces, China’s economy is undergoing a crucial transformation from high-speed growth to high-quality development. As a typical high-energy consumption and high-emission sector, the green and low-carbon transformation of the building materials industry directly affects the optimization of the national energy structure and the realization of ecological goals. However, traditional building material enterprises generally face practical challenges such as low resource utilization efficiency, insufficient digitalization and greening integration of the industrial chain, and weak green innovation momentum. The transformation actions of a single entity are difficult to break through systemic bottlenecks, and it is urgently necessary to establish a dynamic evolution mechanism involving multiple entities in collaboration. This paper aims to explore the evolutionary rules and stability of digital green (DG) transformation strategies of building materials enterprises (BMEs) under multi-agent interactions involving government, universities, and consumers. Centering on BMEs, a four-party evolutionary game model among the government, enterprises, universities, and consumers is constructed, and the evolutionary processes of strategic behaviors are characterized through replicator dynamic equations. Using MATLAB R2022 (Version number: 9.13.0.2049777) bnumerical simulations, this study investigates how key parameters, such as government subsidies, penalty intensity, and consumers’ green preferences, affect the transformation pathways of enterprises. The results reveal that the DG transformation behavior of BMEs is significantly influenced by governmental policy incentives and universities’ knowledge innovation. Stronger subsidies and penalties enhance enterprises’ willingness to adopt proactive DG strategies, while consumers’ green preferences further accelerate transformation through market mechanisms. Among multiple strategic combinations, active DG transformation emerges as the main evolutionarily stable strategy. This study provides a systematic multi-agent collaborative analysis framework for the transformation of BME DG, revealing the mechanisms by which policies, knowledge, and market demands influence enterprise decisions. Thus, it offers theoretical and decision-making references for the green and low-carbon transformation of the building materials industry. Full article

Over-the-Air updates have emerged as a critical competitive frontier in the Software-Defined Vehicle era. While offering value creation opportunities, automakers face strategic uncertainty regarding pricing models (e.g., subscription vs. one-time purchase). To clarify these dynamics, this study develops an evolutionary game model of duopolistic pricing competition. Unlike traditional studies with exogenous payoff assumptions, we innovatively employ the Hotelling model to endogenously derive firm profit functions based on consumer utility maximization. The highlights of this study include: (1) We establish an integrated “static–dynamic” framework connecting micro-level consumer choice with macro-level strategy evolution; (2) We identify that product differentiation is the decisive variable governing market stability; (3) We demonstrate that under moderate differentiation, the market exhibits a robust self-correcting tendency towards “Tacit Collusion” (mutual high pricing). However, simulation results also warn that an asymmetric disruptive strategy by a market leader can override this robustness, forcing the market into a low-profit equilibrium. These findings provide theoretical guidance for automakers to optimize pricing strategies and avoid value-destroying price wars. Full article

In the context of large-scale renewable energy integration driven by China’s dual-carbon goals, and under distribution network scenarios with continuously increasing shares of wind and photovoltaic generation, this paper proposes a source–grid–load–storage coordinated planning method embedded with a multi-agent game mechanism. First, the interest transmission pathways among distributed generation operators (DGOs), distribution network operators (DNOs), energy storage operators (ESOs), and electricity users are mapped, based on which a profit model is established for each stakeholder. Building on this, a coordinated planning framework for active distribution networks (DN) is developed under the assumption of bounded rationality. Through an evolutionary-game process among DGOs, DNOs, and ESOs, and in combination with user-side demand response, the model jointly determines the optimal network reinforcement scheme as well as the optimal allocation of distributed generation (DG) and energy storage system (ESS) resources. Case studies are then conducted to verify the feasibility and effectiveness of the proposed method. The results demonstrate that the approach enables coordinated planning of DN, DG, and ESS, effectively guides users to participate in demand response, and improves both planning economy and renewable energy accommodation. Moreover, by explicitly capturing the trade-offs among multiple stakeholders through evolutionary-game interactions, the planning outcomes align better with real-world operational characteristics. Full article

To identify pancoronaviral inhibitors, we sought to identify peptides that bound the evolutionarily conserved SARS-CoV-2 spike fusion peptide (FP). We screened the NEB PhD-7-mer random combinatorial phage display library against FP, synthesised as a D-peptide, to identify peptides from the L-library to be synthesised as proteolytically resistant D peptides. We selected the top ten peptides that were not seen in another published screen with this library, as these were more likely to be specific. All ten D-peptides had no impact on the infection of Vero-E6/TMPRSS2 cells by SARS-CoV-2. Screening of a proteomic-derived phage display library from the disordered regions of human proteins identified two overlapping 14mer peptides from a region of OTUD1. While a synthetic peptide based on their sequences failed to markedly inhibit viral entry, molecular dynamics structural modelling highlighted a stable binding mode where positive residues on one side of the OTUD1 helix interacted with hydrophobic residues of the FP triple-helical wedge. Thus, while the two phage display strategies failed to yield peptide sequences that are themselves strong inhibitors of viral infection, they led to the development of a computational model that can underpin future designs of potential pancoronaviral FP disruptors. Full article

Agricultural supply chain finance plays a vital role in alleviating the financing constraints faced by agricultural business entities in developing countries and promoting inclusive and sustainable agricultural development. However, issues such as high operational risks, weak credit foundations, and insufficient risk safeguards among stakeholders in the agricultural supply chain have hindered its long-term stability. From the perspective of cooperative sustainability, this study develops a tripartite evolutionary game model involving agricultural enterprises, financial institutions, and farmers to explore the behavioral dynamics and evolutionary stability of their strategies. Using the Fuping mushroom supply chain as a case, Matlab-based simulation analysis reveals that the three-party strategy combinations failed to converge to an evolutionarily stable strategy (ESS) but instead exhibited dynamic changes characterized by non-periodic oscillations. Sensitivity analysis further demonstrates that farmers’ credit behavior is a key determinant of the sustainable operation of the supply chain financing system, while enhancing enterprises’ guarantee willingness can effectively mitigate farmers’ default risk. Moreover, stronger cooperative relationships between enterprises and farmers improve the overall resilience and stability of the system. The findings provide practical insights for building sustainable and resilient agricultural financial ecosystems, emphasizing the need to introduce third-party guarantee institutions, strengthen credit constraint systems, and design incentive mechanisms that promote long-term cooperation among stakeholders. Full article

High-quality development highlights the importance of environmental protection and green low-carbon development. The high-quality development of the manufacturing industry is not only the key content for achieving green transformation, but also an important cornerstone for building a modern national industrial system. Current research focuses on companies and governments, ignoring the important value of suppliers and consumers. As a result, existing mechanisms have failed to deliver the desired results. This paper constructs an evolutionary game model involving manufacturing enterprises, local governments, suppliers, and consumers, and systematically analyzes the strategy selection process of the four participating populations. On this basis, the impact of exogenous and endogenous factors on the evolutionarily stable strategy is studied at the microscopic level using numerical simulation methods. The results show that (1) increasing any of the endogenous factors, such as innovative capability, organization building, and industrial resources, can accelerate the evolution of manufacturing enterprises evolve to smart upgrade strategy. (2) Increasing any one of the exogenous factors, such as policy environment, industrial cooperation, and market demand, can accelerate the rate at which manufacturing enterprises choose to adopt the strategy of smart upgrade. The purpose of this paper is to provide a theoretical reference for the behavioral strategies of manufacturing enterprises, and to provide a realistic reference for local governments to build a mechanism to promote the high-quality development of the manufacturing industry. Full article

The increasing reliance on light-based antimicrobial technologies, such as antimicrobial blue light (aBL) and antimicrobial photodynamic inactivation (aPDI), underscores the urgent need to comprehend bacterial survival strategies beyond conventional resistance. Two key phenotypes—tolerance and resilience—have emerged as critical but often conflated mechanisms by which bacteria withstand oxidative and photodynamic stress. While tolerance refers to delayed bacterial killing without changes in MIC, resilience encompasses the active restoration of cellular function after transient stress exposure. Both phenomena may impair treatment outcomes and contribute to long-term persistence, even in the absence of genetic resistance. This review dissects the molecular mechanisms underlying tolerance and resilience, with a focus on their relevance to bacterial responses to reactive oxygen species generated by light-based or chemical stressors. The regulatory and effector overlap between these phenotypes is examined, including antioxidant defense systems, DNA repair pathways, and metabolic rewiring. Furthermore, the role of phenotypic heterogeneity and cross-stress protection in blurring the boundary between survival and recovery is discussed, highlighting challenges in experimental interpretation. Finally, the implications of these adaptive strategies are evaluated in the context of antimicrobial efficacy and safety, with an emphasis on kinetic assays and multidimensional profiling as tools to capture complex treatment outcomes. Clarifying the distinction between tolerance and resilience may help guide the development of robust and evolutionarily stable antimicrobial phototherapies. Full article

Vertical alliances within agricultural supply chains serve as critical institutional vehicles for deepening triple-sector integration (primary–secondary–tertiary) in rural economies, driving agricultural modernization, and advancing rural revitalization. However, sustaining alliance stability constitutes a complex dynamic process wherein inadequate stakeholder engagement and collaborative failures frequently precipitate alliance instability or even dissolution. Existing scholarship exhibits limited systematic examination of the micro-mechanisms and regulatory pathways through which multi-agent strategic interactions affect alliance stability from a dynamic evolutionary perspective. To address this gap, this research focuses on China’s core agricultural innovation vehicle—the Agricultural Industrialization Consortium—and examines the tripartite structure of “Leading Enterprise–Family Farm–Integrated Agricultural Service Providers.” We construct a tripartite evolutionary game model to systematically analyze (1) the influence mechanisms governing cooperative strategy selection, and (2) the regulatory effects of key parameters on consortium stability through strategic stability analysis and multi-scenario simulations. Our key findings are as follows: Four strategic equilibrium scenarios emerge under specific conditions, with synergistic parameter optimization constituting the fundamental driver of alliance stability. Specific mechanisms are as follows: (i) compensation mechanisms effectively mobilize leading enterprises under widespread defection, though excessive penalties erode reciprocity principles; (ii) strategic reductions in benefit sharing ratios coupled with moderate factor value-added coefficients are critical for reversing leading enterprises’ defection; (iii) dual adjustment of cost sharing and benefit sharing coefficients is necessary to resolve bilateral defection dilemmas; and (iv) synchronized optimization of compensation, cost sharing, benefit sharing, and value-added parameters represents the sole pathway to achieving stable (1,1,1) full-cooperation equilibrium. Critical barriers include threshold effects in benefit sharing ratios (defection triggers when shared benefits > cooperative benefits) and the inherent trade-off between penalty intensity and alliance resilience. Consequently, policy interventions must balance immediate constraints with long-term cooperative sustainability. This study extends the application of evolutionary game theory in agricultural organization research by revealing the micro-level mechanisms underlying alliance stability and providing a novel analytical framework for addressing the ‘strategy–equilibrium’ paradox in multi-agent cooperation. Our work not only offers new theoretical perspectives and methodological support for understanding the dynamic stability mechanisms of agricultural vertical alliances but also establishes a substantive theoretical foundation for optimizing consortium governance and promoting long-term alliance stability. Full article

This paper introduces a novel four-party evolutionary game model to analyze cooperation dynamics in High-Value Mixed Cropping (HMC) systems integrating non-pesticide cacao, cashew nut, and free-range chicken farming within circular and sharing economy frameworks. The model uniquely examines strategic interactions among local government and three farming family types (cacao, cashew, and chicken), incorporating both regulatory mechanisms and cooperative behaviors. Through rigorous stability analysis and MATLAB simulations based on empirical data from Southeast Vietnam, we identify precise conditions for Evolutionarily Stable Strategies (ESSs) that sustain long-term cooperation. Our results demonstrate that government incentives (subsidies, technical support) and reputational sanctions critically shape farmers’ and consumers’ payoffs, thereby steering the system toward collective action equilibria. In particular, increasing the strength of positive incentives or reputational benefits enlarges the basin of attraction for full-cooperation ESSs, regardless of initial strategy distributions. Conversely, overly punitive sanctions can destabilize collaborative outcomes. These findings underscore the pivotal role of well-balanced policy instruments in fostering resilience, innovation, and resource circulation within rural agroecosystems. Finally, we propose targeted policy recommendations, such as graduated subsidy schemes, participatory monitoring platforms, and cooperative branding initiatives, to reinforce circular economy practices and accelerate progress toward the United Nations Sustainable Development Goals. Full article

AI-powered health assistants offer promising opportunities to enhance health management among older adults. However, real-world uptake remains limited, not only due to individual hesitation, but also because of complex interactions among users, platforms, and public policies. This study investigates the dynamic behavioral mechanisms behind adoption in aging populations using a tripartite evolutionary game model. Based on replicator dynamics, the model simulates the strategic behaviors of older adults, platforms, and government. It identifies evolutionarily stable strategies, examines convergence patterns, and evaluates parameter sensitivity through a Jacobian matrix analysis. Results show that when adoption costs are high, platform trust is low, and government support is limited, the system tends to converge to a low-adoption equilibrium with poor service quality. In contrast, sufficient policy incentives, platform investment, and user trust can shift the system toward a high-adoption state. Trust coefficients and incentive intensity are especially influential in shaping system dynamics. This study proposes a novel framework for understanding the co-evolution of trust, service optimization, and institutional support. It emphasizes the importance of coordinated trust-building strategies and layered policy incentives to promote sustainable engagement with AI health technologies in aging societies. Full article

Green building materials play a vital role in mitigating the significant carbon emissions produced by the construction industry. However, the widespread presence of greenwashing, where firms falsely portray their products or practices as environmentally friendly, presents a critical obstacle to the adoption of genuinely sustainable materials. The risk of collusion between building material enterprises and certification institutions further exacerbates this challenge by undermining trust in green certification processes. To investigate these issues, this study develops an evolutionary game model that captures the strategic interactions between building material enterprises and certification institutions. The model incorporates the behavioral assumptions of prospect theory, specifically bounded rationality, loss aversion, and diminishing sensitivity, to reflect the real-world decision-making behavior of the involved actors. The findings reveal three evolutionarily stable strategies (ESS) within the system. First, a higher initial willingness by both enterprises and certifiers to engage in ethical practices increases the likelihood of convergence to an optimal and stable outcome. Second, a greater degree of diminishing sensitivity in the value function promotes the adoption of authentic green behavior by enterprises. In contrast, a lower degree of diminishing sensitivity encourages certification institutions to refrain from collusion. Third, although the loss aversion coefficient does not directly affect strategy selection, higher levels of loss aversion lead to stronger preferences for green behavior among enterprises and noncollusive behavior among certifiers. This research makes a novel theoretical contribution by introducing prospect theory into the analysis of greenwashing behavior in the building materials sector. It also provides actionable insights for improving regulatory frameworks and certification standards to mitigate greenwashing and enhance institutional accountability. Full article

Antibiotic pressure exerts profound effects on bacterial physiology, not limited to classical genetic resistance mechanisms. Increasing evidence highlights the ability of pathogens to undergo metabolic rewiring—an adaptive, reversible reorganization of core metabolic pathways that promotes survival under antimicrobial stress. This review provides a comprehensive analysis of antibiotic-induced metabolic adaptations, encompassing glycolysis, the tricarboxylic acid cycle, fermentation, redox balance, amino acid catabolism, and membrane biosynthesis. We critically examine how diverse antibiotic classes—including β-lactams, aminoglycosides, quinolones, glycopeptides, polymyxins, and antimetabolites—interact with bacterial metabolism to induce tolerance and persistence, often preceding stable resistance mutations. In parallel, we explore the ecological and host-derived signals—such as immunometabolites and quorum sensing—that modulate these metabolic responses. Therapeutically, targeting metabolic pathways offers promising strategies to potentiate antibiotic efficacy, including enzyme inhibition, metabolic adjuvants, and precision-guided therapy based on pathogen metabolic profiling. By framing metabolic plasticity as a dynamic and evolutionarily relevant phenomenon, this review proposes a unifying model linking transient tolerance to stable resistance. Integrating metabolic rewiring into antimicrobial research, clinical diagnostics, and therapeutic design represents a necessary paradigm shift in combating bacterial persistence and resistance. Full article

This study employs an evolutionary game theory framework to analyze the interactive learning, imitation, and strategic evolution among multiple actors within China’s aquatic product traceability system. It focuses on four types of strategic interactions: between fishers and the government, fishers and consumers, fishers who adopt the traceability system and those who do not, and between consumers who purchase traceable aquatic products and those who do not. The evolutionarily stable strategies and equilibrium outcomes in each game depend on the net benefits obtained and the various costs borne by each party. Among these factors, transaction costs within the traceability system play a particularly critical role in shaping stakeholder behavior. The lower the transaction costs, the more likely stakeholders are to adopt strategies that support or enhance the functioning of the system. Therefore, reducing the operational and transaction costs of the traceability system should be a key policy focus for the government. This includes efforts in policy and regulatory development, platform and infrastructure construction, and the improvement of information exchange mechanisms to foster sustainable development in aquaculture. Full article