Search Results (371)

Coastal ecosystems are increasingly threatened by climate change and anthropogenic pressures, which can disrupt microbial communities and favor the emergence of pathogenic organisms. In this study, we applied metagenomic analysis to characterize fungal communities in sediment samples from an urban mangrove subjected to environmental stress. The results revealed a fungal community with reduced richness—28% lower than expected for similar ecosystems—likely linked to physicochemical changes such as heavy metal accumulation, acidic pH, and eutrophication, all typical of urbanized coastal areas. Notably, we detected an increase in potentially pathogenic genera, including Candida, Aspergillus, and Pseudoascochyta, alongside a decrease in key saprotrophic genera such as Fusarium and Thelebolus, indicating a shift in ecological function. The fungal assemblage was dominated by the phyla Ascomycota and Basidiomycota, and despite adverse conditions, symbiotic mycorrhizal fungi remained present, suggesting partial resilience. A considerable fraction of unclassified fungal taxa also points to underexplored microbial diversity with potential ecological or health significance. Importantly, this study does not aim to compare pristine and contaminated environments, but rather to provide a sanitary alert by identifying the presence and potential proliferation of pathogenic fungi in a degraded mangrove system. These findings highlight the sensitivity of mangrove fungal communities to environmental disturbance and reinforce the value of metagenomic approaches for monitoring ecosystem health. Incorporating fungal metagenomic surveillance into environmental management strategies is essential to better understand biodiversity loss, ecological resilience, and potential public health risks in degraded coastal environments. Full article

In Taiwan, hydro-meteorological data are fragmented across multiple agencies, limiting the effectiveness of coordinated flood response. To address this challenge and the increasing uncertainty associated with extreme rainfall, a real-time disaster prevention platform has been developed. This system integrates multi-source data and geospatial information through a cluster-based architecture to enhance pluvial flood management. Built on a Service-Oriented Architecture (SOA) and incorporating Internet of Things (IoT) technologies, AI-based convolutional neural networks (CNNs), and 3D drone mapping, the platform enables automated alerts by linking sensor thresholds with real-time environmental data, facilitating synchronized operational responses. Deployed in New Taipei City over the past three years, the system has demonstrably reduced flood risk during severe rainfall events. Region-specific action thresholds and adaptive strategies are continually refined through feedback mechanisms, while integrated spatial and hydrological trend analyses extend the lead time available for emergency response. Full article

Malignant mediastinal tumors are a group representing some of the most demanding oncological challenges for early, multi-level, and successful management. The timely identification of any suspicious clinical symptomatology is urgent in achieving an accurate, staged histological diagnosis, in order to follow up with an equally detailed medical therapeutic plan (interventional or not) and determine the principal goals regarding efficient overall treatment in these patients. We report a case of a 24-year-old male patient with an incident-free prior medical history. An initial chest X-ray was performed after the patient reported short-term, consistent moderate chest pain symptomatology, early work fatigue, and shortness of breath. The following imaging procedures (chest CT, PET-CT) indicated the presence of an anterior mediastinal mass (meas. ~11 cm × 10 cm × 13 cm, SUV: 8.7), applying additional pressure upon both right heart chambers. The Alpha-Fetoprotein (aFP) blood levels had exceeded at least 50 times their normal range. Two consecutive diagnostic attempts with non-specific histological results, a negative-for-malignancy fine-needle aspiration biopsy (FNA-biopsy), and an additional tumor biopsy, performed via mini anterior (R) thoracotomy with “suspicious” cellular gatherings, were performed elsewhere. After admission to our department, an (R) Video-Assisted Thoracic Surgery (VATS) was performed, along with multiple tumor biopsies and moderate pleural effusion drainage. The tumor’s measurements had increased to DMax: 16 cm × 9 cm × 13 cm, with a severe degree of atelectasis of the Right Lower Lobe parenchyma (RLL) and a pressure-displacement effect upon the Superior Vena Cava (SVC) and the (R) heart sinus, based on data from the preoperative chest MRA. The histological report indicated elements of a combined, non-seminomatous germ-cell mediastinal tumor, posthuberal-type teratoma, and embryonal carcinoma. The imminent chemotherapeutic plan included a “BEP” (Bleomycin^®/Cisplatin^®/Etoposide^®) scheme, which needed to be modified to a “VIP” (Cisplatin^®/Etoposide^®/Ifosfamide^®) scheme, due to an acute pulmonary embolism incident. While the aFP blood levels declined, even reaching normal measurements, the tumor’s size continued to increase significantly (DMax: 28 cm × 25 cm × 13 cm), with severe localized pressure effects, rapid weight loss, and a progressively worsening clinical status. Thus, an emergency surgical intervention took place via median sternotomy, extended with a complementary “T-Shaped” mini anterior (R) thoracotomy. A large, approx. 4 Kg mediastinal tumor was extracted, with additional RML and RUL “en-bloc” segmentectomy and partial mediastinal pleura decortication. The following histological results, apart from verifying the already-known posthuberal-type teratoma, indicated additional scattered small lesions of combined high-grade rabdomyosarcoma, chondrosarcoma, and osteosarcoma, as well as numerous high-grade glioblastoma cellular gatherings. No visible findings of the previously discovered non-seminomatous germ-cell and embryonal carcinoma elements were found. The patient’s postoperative status progressively improved, allowing therapeutic management to continue with six “TIP” (Cisplatin^®/Paclitaxel^®/Ifosfamide^®) sessions, currently under his regular “follow-up” from the oncological team. This report underlines the importance of early, accurate histological identification, combined with any necessary surgical intervention, diagnostic or therapeutic, as well as the appliance of any subsequent multimodality management plan. The diversity of mediastinal tumors, especially for young patients, leaves no place for complacency. Such rare examples may manifest, with equivalent, unpredictable evolution, obliging clinical physicians to stay constantly alert and not take anything for granted. Full article

In the context of global demographic aging, falls among the elderly remain a major public health concern, often leading to injury, hospitalization, and loss of autonomy. This study proposes a real-time fall detection system that combines a modern computer vision model, YOLOv11 with integrated pose estimation, and an Artificial Intelligence (AI)-based voice assistant designed to reduce false alarms and improve intervention efficiency and reliability. The system continuously monitors human posture via video input, detects fall events based on body dynamics and keypoint analysis, and initiates a voice-based interaction to assess the user’s condition. Depending on the user’s verbal response or the absence thereof, the system determines whether to trigger an emergency alert to caregivers or family members. All processing, including speech recognition and response generation, is performed locally to preserve user privacy and ensure low-latency performance. The approach is designed to support independent living for older adults. Evaluation of 200 simulated video sequences acquired by the development team demonstrated high precision and recall, along with a decrease in false positives when incorporating voice-based confirmation. In addition, the system was also evaluated on an external dataset to assess its robustness. Our results highlight the system’s reliability and scalability for real-world in-home elderly monitoring applications. Full article

In today’s increasingly interconnected digital world, cyber threats have grown in frequency and sophistication, making intrusion detection systems a critical component of modern cybersecurity frameworks. Traditional IDS methods, often based on static signatures and rule-based systems, are no longer sufficient to detect and respond to complex and evolving attacks. To address these challenges, Artificial Intelligence and machine learning have emerged as powerful tools for enhancing the accuracy, adaptability, and automation of IDS solutions. This study presents a novel, hybrid ensemble learning-based intrusion detection framework that integrates deep learning and traditional ML algorithms with explainable artificial intelligence for real-time cybersecurity applications. The proposed model combines an Artificial Neural Network and Support Vector Machine as base classifiers and employs a Random Forest as a meta-classifier to fuse predictions, improving detection performance. Recursive Feature Elimination is utilized for optimal feature selection, while SHapley Additive exPlanations (SHAP) provide both global and local interpretability of the model’s decisions. The framework is deployed using a Flask-based web interface in the Amazon Elastic Compute Cloud environment, capturing live network traffic and offering sub-second inference with visual alerts. Experimental evaluations using the NSL-KDD dataset demonstrate that the ensemble model outperforms individual classifiers, achieving a high accuracy of 99.40%, along with excellent precision, recall, and F1-score metrics. This research not only enhances detection capabilities but also bridges the trust gap in AI-powered security systems through transparency. The solution shows strong potential for application in critical domains such as finance, healthcare, industrial IoT, and government networks, where real-time and interpretable threat detection is vital. Full article

Vehicular Communication Networks (VCNs) are essential to intelligent transportation systems, where real-time data exchange between vehicles and infrastructure supports safety, efficiency, and automation. However, achieving high Quality of Service (QoS)—especially under delay-sensitive conditions—remains a major challenge due to the high mobility and dynamic topology of vehicular environments. While some efforts have explored routing protocol optimization, few have systematically compared multiple optimization approaches tailored to distinct traffic and delay conditions. This study addresses this gap by evaluating and enhancing two widely used routing protocols, QOS-AODV and GPSR, through their improved versions, CM-QOS-AODV and CM-GPSR. Two distinct optimization models are proposed: the Traffic-Oriented Model (TOM), designed to handle variable and high-traffic conditions, and the Delay-Efficient Model (DEM), focused on reducing latency for time-critical scenarios. Performance was evaluated using key QoS metrics: throughput (rate of successful data delivery), packet delivery ratio (PDR) (percentage of successfully delivered packets), and end-to-end delay (latency between sender and receiver). Simulation results reveal that TOM-optimized protocols achieve up to 10% higher PDR, maintain throughput above 0.40 Mbps, and reduce delay to as low as 0.01 s, making them suitable for applications such as collision avoidance and emergency alerts. DEM-based variants offer balanced, moderate improvements, making them better suited for general-purpose VCN applications. These findings underscore the importance of traffic- and delay-aware protocol design in developing robust, QoS-compliant vehicular communication systems. Full article

Climate change and air pollution are reshaping viral circulation patterns and increasing host vulnerability, amplifying the burden of respiratory illness in early childhood. This narrative review synthesizes current evidence on how environmental exposures, particularly to nitrogen dioxide, ozone, and fine particulate matter, contribute to the incidence and severity of bronchiolitis, with a focus on biological mechanisms, epidemiological trends, and public health implications. Bronchiolitis remains one of the leading causes of hospitalization in infancy, with Respiratory Syncytial Virus (RSV) being responsible for the majority of severe cases. Airborne pollutants penetrate deep into the airways, triggering inflammation, compromising mucosal defenses, and impairing immune function, especially in infants with pre-existing vulnerabilities. These interactions can intensify the clinical course of viral infections and contribute to more severe disease presentations. Children in urban areas exposed to high levels of traffic-related emissions are disproportionately affected, underscoring the need for integrated public health interventions. These include stricter emission controls, urban design strategies to reduce exposure, and real-time health alerts during pollution peaks. Prevention strategies should also address indoor air quality and promote risk awareness among families and caregivers. Further research is needed to standardize exposure assessments, clarify dose–response relationships, and deepen our understanding of how pollution interacts with viral immunity. Bronchiolitis emerges as a sentinel condition at the crossroads of climate, environment, and pediatric health, highlighting the urgent need for collaboration across clinical medicine, epidemiology, and environmental science. Full article

We present a case of fatal necrotizing Staphylococcus aureus pneumonia with underlying influenza A (H3) infection. Next-generation-sequencing-based analysis revealed that the S. aureus isolate harbored the newly recognized exfoliative toxin etE2 gene. Molecular epidemiologic analysis showed that the isolate belonged to the MSSA ST152 lineage, harboring PVL genes and edinB co-located to etE2 as distinctive virulence factors. The etE2 gene is present in all isolates of this lineage co-located to the exotoxin gene edinB, both implicated in the destruction of tissue integrity. We alert as to the global emergence of this lineage causing serious infections in patients. Full article

Pharmacogenomics (PGx) has emerged as a powerful tool to personalize drug selection and dosing based on a patient’s genetic profile. However, there are a range of challenges that impede uptake in current clinical practice. For example, clinicians often express frustration with commercially available PGx panel tests, which fail to consistently include all key actionable PGx genes (according to the Clinical Pharmacogenetics Implementation Consortium (CPIC), Food and Drug Administration (FDA) PGx guidelines, or The Dutch Pharmacogenetics Working Group (DPWG) guidelines) and instead are too long with clinically unimportant information (unvalidated genotypes). Additionally, the lack of EMR integration, clinician education and awareness of the benefits of PGx impedes uptake. This paper examines key challenges identified in clinical practice and proposes future directions, focusing on limiting PGx reports to essential data, providing point-of-prescription alerts, and establishing reimbursement pathways that encourage adoption. Future directions include leveraging large language models, integrating point-of-prescription alerts and phenoconversion calculators into the electronic medical record, increasing the genomic diversity of PGx study populations, and streamlining coverage by payers. Full article

In recent years, the world has seen a significant increase in extreme weather events, such as floods, hurricanes, and storms, which have caused extensive damage to infrastructure and communities. These events result from natural phenomena and human-induced factors, including climate change and natural climate variations. For instance, the floods in Europe in 2024 and the hurricanes in the United States have highlighted the vulnerability of urban and rural areas. These extreme events are often unpredictable and pose considerable challenges for spatial planning and risk management. This study explores an innovative approach that employs machine learning and Markov chains to enhance spatial planning and predict flood risk areas. By utilizing data such as weather records, land use and land cover (LULC) information, topographic LIDAR data, and advanced predictive models, the study aims to identify the most vulnerable areas and provide recommendations for risk mitigation. The results indicate that integrating these technologies can improve forecasting accuracy, thereby supporting more informed decisions in land management. Given the effects of climate change and the increasing frequency of extreme events, adopting advanced forecasting and planning tools is crucial for protecting communities and reducing economic and social damage. This method was applied to the Calopinace area, also known as the Calopinace River or Fiumara della Cartiera, which crosses Reggio Calabria and is notorious for its historical floods. It can serve as part of an early warning system, enabling alerts to be issued throughout the monitored area. Furthermore, it can be integrated into existing emergency protocols, thereby enhancing the effectiveness of disaster response. Future research could investigate incorporating additional data and AI techniques to improve accuracy. Full article

Background: Rapid identification and treatment of stroke are essential for the patient. Our objective was to determine the diagnostic utility of glial fibrillary acidic protein (GFAP) and ubiquitin C-terminal hydrolase L1 (UCH-L1) in the emergency department to identify and differentiate acute stroke within 4.5 h of symptom onset in patients admitted with a stroke code alert. Methods: This study included 85 patients with a “code stroke alert” upon arrival at the emergency department. Individuals were grouped in two categories: patients with stroke (including 69 patients) and patients without stroke (including 16 patients). The research was conducted at the Emergency Municipal Clinical Hospital in Timișoara, Romania, the county’s second-largest hospital, which lacks a neurologist and a dedicated stroke unit. Results: No significant differences were observed between the two groups (with stroke and without stroke) regarding most demographic or admission parameters. Significant differences were observed for the biomarkers GFAP (142.91 ± 102.19 pg/mL in patients with acute stroke vs. 37.76 ± 19.92 pg/mL in patients without stroke (p < 0.001)) and UCH-L1 (1307.68 ± 967.54 pg/mL in stroke patients vs. 189.81 ± 92.69 pg/mL in patients without stroke (p < 0.001)). Within the stroke group, 37 patients had acute ischemic stroke, while 32 patients were diagnosed with hemorrhagic stroke based on brain CT imaging. GFAP achieved an accuracy of 94.2% for differentiating hemorrhagic from ischemic stroke, with a cut-off value of 77.15 pg/mL. Conclusions: GFAP excellently differentiated acute stroke from stroke mimics, with high sensitivity, perfect specificity, and strong predictive values. Integrating GFAP and UCH-L1 measurements into emergency protocols may enhance stroke diagnosis, optimize patient triage, and ultimately improve outcomes by facilitating the faster initiation of appropriate therapies. Full article

The rapid integration of AI into IoT systems has outpaced the ability to explain and audit automated decisions, resulting in a serious transparency gap. We address this challenge by proposing a blockchain-based framework to create immutable audit trails of AI-driven IoT decisions. In our approach, each AI inference comprising key inputs, model ID, and output is logged to a permissioned blockchain ledger, ensuring that every decision is traceable and auditable. IoT devices and edge gateways submit cryptographically signed decision records via smart contracts, resulting in an immutable, timestamped log that is tamper-resistant. This decentralized approach guarantees non-repudiation and data integrity while balancing transparency with privacy (e.g., hashing personal data on-chain) to meet data protection norms. Our design aligns with emerging regulations, such as the EU AI Act’s logging mandate and GDPR’s transparency requirements. We demonstrate the framework’s applicability in two domains: healthcare IoT (logging diagnostic AI alerts for accountability) and industrial IoT (tracking autonomous control actions), showing its generalizability to high-stakes environments. Our contributions include the following: (1) a novel architecture for AI decision provenance in IoT, (2) a blockchain-based design to securely record AI decision-making processes, and (3) a simulation informed performance assessment based on projected metrics (throughput, latency, and storage) to assess the approach’s feasibility. By providing a reliable immutable audit trail for AI in IoT, our framework enhances transparency and trust in autonomous systems and offers a much-needed mechanism for auditable AI under increasing regulatory scrutiny. Full article

Battery electric vehicles (BEVs) are seeing widespread adoption globally due to technological improvements, lower manufacturing costs, and supportive policies aimed at reducing greenhouse gas emissions. Governments have introduced incentives such as purchase subsidies and investments in charging infrastructure, while automakers continue to broaden their electric vehicle portfolios. Although BEVs show high overall safety performance comparable to internal combustion engine vehicles (ICEVs), they also raise distinct safety challenges that merit policy attention. This review synthesizes the current literature on safety concerns associated with BEVs, with particular attention to fire risks, vehicle weight, low-speed noise levels, and unique driving characteristics. Fire safety remains a significant issue, as lithium-ion battery fires, although less frequent than those in ICEVs, tend to be more severe and difficult to manage. Strategies such as improved thermal management, fire enclosures, and standardized response protocols are essential. BEVs are typically heavier than ICEVs, affecting crash outcomes and braking performance. These risks are especially important for interactions with pedestrians and smaller vehicles. Quiet operation at low speeds can also reduce pedestrian awareness, prompting regulations for vehicle sound alerts. Together, these issues highlight the need for policies that address both emerging safety risks and the evolving nature of BEV technology. Full article

The challenges and increasing number of elderly individuals requiring remote monitoring at home highlight the need for technological innovations. This study devised an eHealth mobile application designed to detect abnormal movement behavior and alert caregivers when a lack of movement is detected for an abnormal period. By utilizing the built-in accelerometer of a conventional mobile phone, an application was developed to accurately record movement patterns and identify active and idle states. Fuzzy logic, an artificial intelligence (AI)-inspired paradigm particularly effective for real-time reasoning under uncertainty, was integrated to analyze activity data and generate timely alerts, ensuring rapid response in emergencies. The approach reduced development costs while leveraging the widespread familiarity with mobile phones, facilitating easy adoption. The approach involved collecting real-time accelerometry data, analyzing movement patterns using fuzzy logic-based inferencing, and implementing a rule-based decision system to classify user activity and detect inactivity. This pilot study primarily validated the devised fuzzy logic method and the functional prototype of the mobile application, demonstrating its potential to leverage universal smartphone accelerometers for accessible remote monitoring. Using fuzzy logic, temporal and behavioral symmetry in movement patterns were adapted to detect asymmetric anomalies, e.g., abnormal inactivity or falls. The study is particularly relevant considering lonely individuals found deceased in their homes long after dying. By providing real-time monitoring and proactive alerts, this eHealth solution offers a scalable, cost-effective approach to improving elderly care, enhancing safety, and reducing the risk of unnoticed deaths through fuzzy logic. Full article

In early 2025, the Santorini–Amorgos area (Aegean Volcanic Arc, Greece) experienced a seismic swarm, with dozens of M ≥ 4.0 earthquakes and a maximum magnitude of M = 5.2. Beyond its seismological interest, the sequence was notable for triggering rare increased preparedness actions by Greek Civil Protection operational structures in anticipation of an imminent destructive earthquake. These actions included (i) risk communication, (ii) the reinforcement of operational structures with additional personnel and equipment on the affected islands, (iii) updates to local emergency plans, (iv) the dissemination of self-protection guidance, (v) the activation of emergency alert systems, and (vi) volunteer mobilization, including first aid and mental health first aid courses. Although it was in line with contingency plans, public participation was limited. Volunteers helped bridge this gap, focusing on vulnerable groups. The implemented actions in Greece are also compared with increased preparedness during the 2024–2025 seismic swarms in Ethiopia, as well as preparedness before the highly anticipated major earthquake in Istanbul (Turkey). In Greece and Turkey, legal and technical frameworks enabled swift institutional responses. In contrast, Ethiopia highlighted the risks of limited preparedness and the need to embed disaster risk reduction in national development strategies. All cases affirm that preparedness, through infrastructure, planning, communication, and community engagement, is vital to reducing earthquake impacts. Full article