Search Results (23)

Mulberry (Morus alba) is an economically important forest tree species, yet cutting propagation is constrained by low adventitious rooting efficiency. Although ABT, a composite rooting promoter, can improve cutting survival, its molecular basis remains unclear. Here, cuttings of the cultivar Qiangsang 1 were treated with ABT, NAA, or IAA (200–1000 mg/L) and subjected to transcriptome profiling to elucidate how ABT enhances rooting. Hormone-related analyses showed that ABT upregulated GH3 (auxin-amido synthetase) at days 0 and 20, implicating auxin homeostasis. ERF1/2 (ethylene response factors) exhibited a temporal oscillation, with induction at day 10 followed by repression from days 20 to 30, consistent with a shift from developmental programs to defense-related processes. In parallel, JAZ (jasmonate ZIM-domain) genes were downregulated at day 0 and subsequently upregulated; together with CYP94C1, these changes may attenuate jasmonate-associated defense signaling. For cell remodeling and defense coordination, ABT reduced the expression of genes associated with cell-wall rigidity while inducing EXPA11 (expansin) at day 20, potentially facilitating root primordium emergence. Meanwhile, PR-1 (pathogenesis-related protein 1) was transiently upregulated at days 0, 20, and 30, and the concomitant modulation of WRKY transcription factors and RPM1 suggests enhanced defense readiness. Integrative network analysis further indicated that a GH3–ERF1/2–PR-1 module links hormonal and defense cues and may activate BAT1 (energy metabolism) and RBOHB (ROS production) to support adventitious root elongation. Collectively, these results suggest that ABT improves rooting efficiency by reshaping hormonal homeostasis and coordinating cell-wall reconstruction with a pre-activated defense state, thereby providing a conceptual framework for balancing root induction and defense responses during vegetative propagation in forest trees. Full article

The interaction of emergent vegetation and three-dimensional (3D) bedforms is essential for understanding turbulent flow dynamics in curved channels. A laboratory investigation can help to collect required data under controlled conditions. Experiments were conducted in a 9.5 m-long, 0.9 m-wide recirculating flume incorporating a 90° bend and a sculpted 3D pool bedform. Artificial rigid vegetation, designed to replicate the hydraulic behavior of natural emergent plants, was installed along both sidewalls. Instantaneous three-dimensional velocities were recorded using an acoustic Doppler velocimeter (ADV) across multiple cross-sections under both bare-bed and vegetated conditions. The results reveal that emergent vegetation markedly increases flow resistance, distorts mean velocity distributions, and suppresses the classical logarithmic velocity profile, particularly within the bend and pool regions. The combined presence of vegetation and the 3D pool bedform amplified turbulence intensity, elevated Reynolds shear stresses, and redistributed turbulent kinetic energy (TKE), which increased by up to sevenfold from the bend entrance to its exit. In vegetated pool sections, Reynolds stresses were approximately 12% greater than under bare-bed conditions, underscoring the synergistic effects of vegetation drag, secondary circulation, and flow separation in producing anisotropic turbulence. These findings highlight the importance of incorporating vegetation–bedform interactions in fluvial modeling frameworks, with significant implications for sediment transport prediction, channel stability evaluation, river restoration, and aquatic habitat design. Full article

Riparian and floodplain vegetation play a key role in controlling flow resistance, sediment transport, and channel morphology, shaping the dynamics of riverine ecosystems. Accurately representing these vegetation–flow–sediment interactions in numerical models is essential for predicting system responses and supporting sustainable river management. This study introduces an enhanced biomorphodynamic model in the open-source framework openTELEMAC, which combines multiple vegetation friction approaches with a method for predicting sediment transport in vegetated flows. The modular structure of the framework enables flexible configurations for different vegetation types (rigid or flexible) and flow conditions (emergent or submerged) by selecting suitable vegetation friction approaches, improving usability and extending model applicability. Model performance is evaluated using two laboratory experiments on bedload transport through emergent and submerged rigid vegetation. Simulations reproduce the measured bed and water surface profiles with high accuracy, yielding low goodness-of-fit errors (RMSE ≈ 0.5–1.8 cm, MAE ≈ 0.5–1.6 cm). The results highlight the sensitivity of predictions to vegetational input parameters such as the drag coefficient. Overall, the enhanced biomorphodynamic model advances the representation of vegetation–sediment interactions and provides an adaptable, open-source tool for eco-hydraulic and morphodynamic research. Full article

Emergent vegetation in river corridors influences both the flow structure and subsequent fluvial processes. This investigation aimed to analyze the impact of the bending and vegetation components in a sharply curved open channel on the flow field. Experiments were undertaken in a meandering flume (0.9 m wide, wavelength of 3.2 m, and a sinuosity of 1.05) with a 90-degree bend at the end of it, with and without vegetation, to achieve this goal. The individual vegetation elements arranged across the 90-degree bend of the flow channel were physically modelled using rigid plastic stems (of 5 mm and 10 mm diameters). Analysis of the findings from the flow velocimetry, taken at five cross-sections oriented at angles of 0°, 30°, 45°, 60°, and 90°, along the 90-degree bend indicates that as the plant density increases, the effect of centrifugal force from the channel’s bend on the cross-sectional flow patterns decreases. At the same time, the restricting influence of vegetation on lateral momentum transfer becomes more pronounced. Specifically, for increasing vegetation density: (a) higher transverse and vertical velocities are observed (increased by 4.35% and 9.68% for 5 mm and 10 mm reed vegetation, respectively, compared to the non-vegetated case); (b) greater turbulence intensity is seen in the transverse flow direction, along with increased turbulent kinetic energy (TKE); and (c) reduced near-bed Reynolds stresses are found. The average transverse flow velocity for the non-vegetated case is 18.19% of the longitudinal flow velocity and the average vertical velocity for the non-vegetated case and 5 mm and 10 mm reed vegetation is 3.24%, 3.6%, and 5.44% of the longitudinal flow velocity, respectively. Full article

On the edges of rivers where the flow velocity is low, aquatic plants flourish, with emergent rigid herbs being the most common. Since the flow structures of vegetated flow are strongly influenced by vegetation distribution patterns, homogeneous and heterogeneous canopies are defined based on the characteristics of vegetation distribution. A review summarizing recent advances in flow structures under the influence of different types of canopy arrangements, including ribbon-like homogeneous canopies, ribbon-like heterogeneous canopies, and patched heterogeneous canopies, is needed. Their flow development process, shear layer properties, coherent structure features, and momentum exchange characteristics are summarized, and a future research agenda for an in-depth understanding of the interactions between vegetation and flow is also highlighted. Full article

Curved channels and aquatic vegetation are commonly present in the riverine environment. In this study, the effects of vegetation density and distribution on the hydrodynamic characteristics of a mixed layer developed over a 180-degree curved channel were investigated through flume experiments. Wooden sticks were used to simulate rigid vegetation distributed along the half side of the channel, and a 200 Hz acoustic Doppler velocimeter (ADV) was employed to measure the three-dimensional instantaneous velocity at five selected cross sections along the curved channel. Experimental results show that the vegetation covering the half of the channel significantly affects the hydrodynamic structure of the curved channel flow, and the unequal vegetation resistance induces the K-H instability at the vegetation and non-vegetation interface, resulting in a standard hyperbolic tangent function of streamwise velocity distribution along the lateral direction. The influence of curve position on turbulence kinetic energy is far greater than that of vegetation density and vegetation distribution. The peak value of turbulent kinetic energy is comprehensively affected by vegetation density and distribution, and the peak position of turbulent kinetic energy at the interface is changed by different vegetation distribution. The combined effect of the curve and the partly covered vegetation increases the mixing between the water bodies, enhancing turbulent kinetic energy, and vegetation along the concave bank plays a more significant role. For turbulent bursting, the inward and outward interactions are mainly bursting events in the vegetation area, while ejections and sweeps are dominant in the non-vegetation area. However, the critical vegetation condition to initiate large-scale coherent structure (LSS) in the mixed layer and the influence of flexible vegetation need to be further studied in the future. Full article

The effects of partially emergent rigid vegetation on the hydrodynamics of a curved open-channel confluence flow were simulated using OpenFOAM. The numerical model using the Volume of Fluid method and the RNG k-ε turbulence model in the Reynolds-averaged Navier–Stokes equations was first validated by existing experimental data with good agreement. Then the characteristics of hydrodynamics were analyzed in aspects of separation zone, water level, streamwise velocities, secondary flows, bed shear stress and flow resistance. Some main conclusions can be drawn from the results. Compared to the non-vegetated cases, the separation zones in vegetated cases are smaller in both length and width. With higher vegetation Solid Volume Fraction (SVF), the separation zone is divided into two parts, a smaller one right after the confluence point and a larger one on the second half of the curved reach after the confluence. The main circulation cell shrinks and the circulation near the concave bank moves towards the channel midline. The differences in velocities and bed shear stress between the convex and concave banks become larger with a higher SVF. Under the same SVF, a larger vegetation density has more disturbance on the tributary than a larger stem diameter. Full article

The paper presents the results relying on 12 experimental M2 water profiles observed in a flume with emergent stems in a square arrangement. The authors used a recently proposed approach to determine the drag coefficients in the flow direction. Since these showed a behavior difficult to interpret, the authors first computed for each profile the best value of the Manning coefficient for the profile simulation and then the drag coefficients. With the help of a classical dimensional analysis, a regression equation was found to predict the drag coefficients, and these were used to simulate the observed profiles with good results. Full article

A few decades ago, river erosion protective approaches were widely implemented, such as straightening the river course, enhancing riverbed/bank stability with layers of concrete or riprap, and increasing channel conveyance capacity (i.e., overwidening). However, recent research has established that such practices can be tremendously costly and adversely affect the rivers’ ecological health. To alleviate these effects, green river restoration has emerged as a sustainable and environmentally friendly approach that can reduce the negative impact of the riverbed and bank destabilization and flooding. One of the typical green restoration measures, especially for instream habitat improvement, is the establishment of instream vegetation, which leads to a more diversified flow regime, increasing habitat availability and serving as refugia for aquatic species. Within the perspective presented above, flow–vegetation interaction problems for several decades received significant attention. In these studies, rigid rods have commonly been used to simulate these vegetative roughness elements without directly assessing the riverbed destabilization potential. Here, an experimental study is carried out to investigate the effect of different instream vegetation porosity on the near-bed flow hydrodynamics and riverbed destabilization potential for a range of simulated vegetation species. Specifically, the flow field downstream, four distinct simulated vegetation elements is recorded using an acoustic Doppler velocimetry (ADV), assuming about the same solid volume fraction for the different vegetation elements. In addition, bed destabilization potential is assessed by recording with optical means (a He-Ne laser with a camera system) the entrainment rate of a 15 mm particle resting on the uniform bed surface and the number of impulses above a critical value. Results revealed that the number of impulses above a critical value at the normalized distance equal to two is a good indicator for cylinder and five for other vegetation to assess the riverbed destabilization potential. The experimental findings from this study have interesting geomorphological implications regarding the destabilization of the riverbed surface (removal of coarse particles induced by high magnitude turbulent impulses) and the successful establishment of seedlings downstream of instream vegetation. Full article

Even though both fluid mechanics and numerical studies have considerably progressed in the past decades, experimental knowledge remains an important tool for studying the resistance to flow in fluid media where a complex environment dominates the flow pattern. After a comprehensive review of the recent literature on the drag coefficient in open channels with emergent rigid vegetation, this paper presents the results related to 29 experimental accelerated subcritical flow profiles (i.e., M2 type) that were observed in flume experiments with emergent stems in a square arrangement at the University of Calabria (Italy). First of all, we used some of the literature formulas for the drag coefficient, concluding that they were unsatisfactory, probably because of their derivation for uniform or quasi-uniform flow conditions. Then, we tested a recently proposed approach, but when we plotted the drag coefficient versus the stem Reynolds number, the calculated drag coefficients showed an inconclusive behavior to interpret. Thus, we proposed a new approach that considers the calibration of the Manning coefficient for the simulation of the free surface profile, and then the evaluation of the drag coefficients based on the fundamental fluid mechanics equations. With the help of classical dimensional analysis, a regression equation was found to estimate the drag coefficients by means of non-dimensional parameters, which include vegetation density, stem Reynolds number and flow Reynolds number computed using the flow depth as characteristic length. This equation was used to simulate all the 26 observed profiles and, also, 4 experimental literature profiles, and the results were good. The regression equation could be used to estimate the drag coefficient for the M2 profiles in channels with squared stem arrangements, within the range of vegetation densities, flow Reynolds numbers and stem Reynolds numbers of the present study. However, in the case of the three profiles observed by the authors for staggered arrangement, the regression equation gives significantly underestimated flow depths. Full article

Large eddy simulation (combined with the mixture model) and laboratory experiment were used to investigate the impact of emergent and rigid vegetation on the dynamics of downslope gravity currents in stratified environments. The reliability of the numerical model was assessed with the corresponding laboratory measurements. The results show that the vegetation cylinders lead to severe lateral non-uniformity of the current front, causing more evident lobe and cleft structures. In stratified environments, the smaller driving force leads to less propagating velocity until the current separates from the slope. The transition point (from acceleration to deceleration phases) of current velocity appears earlier as the vegetation becomes denser. The peak value of the bulk entrainment coefficient $E_{buik}$ is inversely proportional to the vegetation density, while the final converged value of $E_{buik}$ is proportional to the vegetation density. Vegetation patches make the degree of fluctuation of the instantaneous entrainment coefficient $E_{inst}$ more intense, and even negative values appear locally, indicating that the gravity current is detrained into the ambient fluid. The velocity profiles of gravity current develop multi-peak patterns in stratified environments due to fingering intrusive patterns. Our analysis reveals that as the vegetation density increases, the generated wakes behind vegetation cylinders increase local entrainment and mixing, causing the density of current flow from vegetation to decrease and reach the neutral buoyancy layer of ambient fluids earlier, finally leading to a smaller separation depth. Full article

River vegetation radically modifies the flow field and turbulence characteristics. To analyze the vegetation effects on the flow, most scientific studies are based on laboratory tests or numerical simulations with vegetation stems on smooth beds. Nevertheless, in this manner, the effects of bed sediments are neglected. The aim of this paper is to experimentally investigate the effects of bed sediments in a vegetated channel and, in consideration of that, comparative experiments of velocity measures, performed with an Acoustic Doppler Velocimeter (ADV) profiler, were carried out in a laboratory flume with different uniform bed sediment sizes and the same pattern of randomly arranged emergent rigid vegetation. To better comprehend the time-averaged flow conditions, the time-averaged velocity was explored. Subsequently, the analysis was focused on the energetic characteristics of the flow field with the determination of the Turbulent Kinetic Energy (TKE) and its components, as well as of the energy spectra of the velocity components immediately downstream of a vegetation element. The results show that both the vegetation and bed roughness surface deeply affect the turbulence characteristics. Furthermore, it was revealed that the roughness influence becomes predominant as the grain size becomes larger. Full article

Flow resistance, velocity distribution, and turbulence intensity are significantly influenced by aquatic vegetations (AV) in riparian zones. Understanding the hydraulics of flow with planted floodplains is of great significance for determining the velocity distribution profile and supporting the fluvial processes management. However, the traditional flume experiment method is inefficient. Therefore, the multigroup simultaneous flume test method was carried out to describe the flow patterns affected by emerged rigid (reed and wooden stick) and submerged flexible vegetations (grass and chlorella). The Acoustic Doppler Velocimeter (ADV) was utilized to measure the velocity at one point for different experimental conditions. The results showed that hydraulic features were influenced by different types of vegetation. Furthermore, the relative depth (z/h) was a determining factor of those variations. In addition, the time-averaged velocity distributions of planted floodplains are not logarithmic. Instead, they represented “s-shape” profiles. In detail, for the vegetated floodplains, reed and wood followed an s-shape profile, but for grass and chlorella, they followed reverse s-shape profile. For all cases, turbulence is not isotropic and the change law of turbulence intensity is different in different sections. The flow resistance, turbulence intensities, and Reynold stresses influenced by different types of vegetation were also analyzed. Full article

Vegetation on the banks and flooding areas of watercourses significantly affects energy losses. To take the latter into account, computational models make use of resistance coefficients based on the evaluation of bed and walls roughness besides the resistance to flow offered by vegetation. This paper, after summarizing the classical approaches based on descriptions and pictures, considers the recent advancements related to the analytical methods relative both to rigid and flexible vegetation. In particular, emergent rigid vegetation is first analyzed by focusing on the methods for determining the drag coefficient, then submerged rigid vegetation is analyzed, highlighting briefly the principles on which the different models are based and recalling the comparisons made in the literature. Then, the models used in the case of both emergent and submerged rigid vegetation are highlighted. As to flexible vegetation, the paper reminds first the flow conditions that cause the vegetation to lay on the channel bed, and then the classical resistance laws that were developed for the design of irrigation canals. The most recent developments in the case of submerged and emergent flexible vegetation are then presented. Since turbulence studies should be considered as the basis of flow resistance, even though the path toward practical use is still long, the new developments in the field of 3D numerical methods are briefly reviewed, presently used to assess the characteristics of turbulence and the transport of sediments and pollutants. The use of remote sensing to map riparian vegetation and estimating biomechanical parameters is briefly analyzed. Finally, some applications are presented, aimed at highlighting, in real cases, the influence exerted by vegetation on water depth and maintenance interventions. Full article

Most of the existing works on vegetated flows are based on experimental tests in smooth channel beds with staggered-arranged rigid/flexible vegetation stems. Actually, a riverbed is characterized by other roughness elements, i.e., sediments, which have important implications on the development of the turbulence structures, especially in the near-bed flow zone. Thus, the aim of this experimental study was to explore for the first time the turbulence anisotropy of flows through emergent rigid vegetation on rough beds, using the so-called anisotropy invariant maps (AIMs). Toward this end, an experimental investigation, based on Acoustic Doppler Velocimeter (ADV) measures, was performed in a laboratory flume and consisted of three runs with different bed sediment size. In order to comprehend the mean flow conditions, the present study firstly analyzed and discussed the time-averaged velocity, the Reynolds shear stresses, the viscous stresses, and the vorticity fields in the free stream region. The analysis of the AIMs showed that the combined effect of vegetation and bed roughness causes the evolution of the turbulence from the quasi-three-dimensional isotropy to axisymmetric anisotropy approaching the bed surface. This confirms that, as the effects of the bed roughness diminish, the turbulence tends to an isotropic state. This behavior is more evident for the run with the lowest bed sediment diameter. Furthermore, it was revealed that also the topographical configuration of the bed surface has a strong impact on the turbulent characteristics of the flow. Full article