Sign in to use this feature.

Years

Between: -

Subjects

remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline

Journals

remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline

Article Types

Countries / Regions

remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline

Search Results (805)

Search Parameters:
Keywords = ease allowance

Order results
Result details
Results per page
Select all
Export citation of selected articles as:
10 pages, 1004 KiB  
Article
Removal of Octinoxate, a UV-filter Compound, from Aquatic Environment Using Polydimethylsiloxane Sponge
by Péter Szabó, Zoltán Németh, Ruben Szabó, István Lázár, Zsolt Pirger and Attila Gáspár
Water 2025, 17(15), 2306; https://doi.org/10.3390/w17152306 - 3 Aug 2025
Viewed by 65
Abstract
This work demonstrates the potential of polydimethylsiloxane sponges for removing organic UV filter compounds such as octinoxate from aqueous solutions. The sponges were fabricated using simple templates made of hydrophilic fused or pressed particles (sugar or NaCl salt) with an approximate particle size [...] Read more.
This work demonstrates the potential of polydimethylsiloxane sponges for removing organic UV filter compounds such as octinoxate from aqueous solutions. The sponges were fabricated using simple templates made of hydrophilic fused or pressed particles (sugar or NaCl salt) with an approximate particle size of 0.4 mm. Among the prepared sponges, those templated with sugar cubes or coarse salt exhibited the highest adsorption capacity, effectively adsorbing up to 0.6% of their own mass in octinoxate. The PDMS sponges were fully regenerable, allowing for the complete removal of octinoxate without any detectable changes in their adsorption properties or dry weight. Due to their simple fabrication, ease of handling, ability to float, and reusability, PDMS sponges present an environmentally friendly and low-maintenance alternative to conventional filtration systems for the removal of octinoxate and potentially other UV filter compounds from environmental surface waters and recreational water bodies. Full article
(This article belongs to the Section Water Quality and Contamination)
Show Figures

Figure 1

17 pages, 3738 KiB  
Article
Beyond Spheres: Evaluating Gold Nano-Flowers and Gold Nano-Stars for Enhanced Aflatoxin B1 Detection in Lateral Flow Immunoassays
by Vinayak Sharma, Bilal Javed, Hugh J. Byrne and Furong Tian
Biosensors 2025, 15(8), 495; https://doi.org/10.3390/bios15080495 - 1 Aug 2025
Viewed by 184
Abstract
The lateral flow immunoassay (LFIA) is a widely utilized, rapid diagnostic technique characterized by its short analysis duration, cost efficiency, visual result interpretation, portability and suitability for point-of-care applications. However, conventional LFIAs have limited sensitivity, a challenge that can be overcome by the [...] Read more.
The lateral flow immunoassay (LFIA) is a widely utilized, rapid diagnostic technique characterized by its short analysis duration, cost efficiency, visual result interpretation, portability and suitability for point-of-care applications. However, conventional LFIAs have limited sensitivity, a challenge that can be overcome by the introduction of gold nanoparticles, which provide enhanced sensitivity and selectivity (compared, for example, to latex beads or carbon nanoparticles) for the detection of target analytes, due to their optical properties, chemical stability and ease of functionalization. In this work, gold nanoparticle-based LFIAs are developed for the detection of aflatoxin B1, and the relative performance of different morphology particles is evaluated. LFIA using gold nano-labels allowed for aflatoxin B1 detection over a range of 0.01 ng/mL–100 ng/mL. Compared to spherical gold nanoparticles and gold nano-flowers, star-shaped gold nanoparticles show increased antibody binding efficiency of 86% due to their greater surface area. Gold nano-stars demonstrated the highest sensitivity, achieving a limit of detection of 0.01ng/mL, surpassing the performance of both spherical gold nanoparticles and gold nano-flowers. The use of star-shaped particles as nano-labels has demonstrated a five-fold improvement in sensitivity, underscoring the potential of integrating diverse nanostructures into LFIA for significantly improving analyte detection. Moreover, the robustness and feasibility of gold nano-stars employed as labels in LFIA was assessed in detecting aflatoxin B1 in a wheat matrix. Improved sensitivity with gold nano-stars holds promise for applications in food safety monitoring, public health diagnostics and rapid point-of-care diagnostics. This work opens the pathway for further development of LFIA utilizing novel nanostructures to achieve unparallel precision in diagnostics and sensing. Full article
Show Figures

Figure 1

26 pages, 3844 KiB  
Article
A No-Code Educational Platform for Introducing Internet of Things and Its Application to Agricultural Education
by George Lagogiannis and Avraam Chatzopoulos
IoT 2025, 6(3), 42; https://doi.org/10.3390/iot6030042 - 31 Jul 2025
Viewed by 220
Abstract
This study introduces a no-code educational platform created to introduce Internet of Things (IoT) to university students who lack programming experience. The platform allows users to set IoT sensor nodes, and create a wireless sensor network through a simple graphical interface. Sensors’ data [...] Read more.
This study introduces a no-code educational platform created to introduce Internet of Things (IoT) to university students who lack programming experience. The platform allows users to set IoT sensor nodes, and create a wireless sensor network through a simple graphical interface. Sensors’ data can be sent to cloud services but they can also be stored locally, which makes our platform particularly realistic in fieldwork settings where internet access may be limited. The platform was tested in a pilot activity within a university course that previously covered IoT only in theory and was evaluated using the Technology Acceptance Model (TAM). Results showed strong student engagement and high ratings for ease of use, usefulness, and future use intent. These findings suggest that a no-code approach can effectively bridge the gap between IoT technologies and learners in non-engineering fields. Full article
Show Figures

Figure 1

29 pages, 9521 KiB  
Article
The Chemical Fingerprint of Smokeless Powders: Insights from Headspace Odor Volatiles
by Miller N. Rangel, Andrea Celeste Medrano, Haylie Browning, Shawna F. Gallegos, Sarah A. Kane, Nathaniel J. Hall and Paola A. Prada-Tiedemann
Powders 2025, 4(3), 21; https://doi.org/10.3390/powders4030021 - 29 Jul 2025
Viewed by 700
Abstract
Smokeless powders are a commonly used low explosive within the ammunition industry. Their ease of purchase has allowed criminals to use these products to build improvised explosive devices. Canines have become a vital tool in locating such improvised devices. With differing fabrication processes, [...] Read more.
Smokeless powders are a commonly used low explosive within the ammunition industry. Their ease of purchase has allowed criminals to use these products to build improvised explosive devices. Canines have become a vital tool in locating such improvised devices. With differing fabrication processes, one of the most difficult challenges for canine handlers is the optimal selection of training aids to choose as odor targets to allow for broad generalization. Several studies have been underway to understand the chemical odor characterization of smokeless powders, which can help provide canine teams with essential information to understand odor signatures from powder varieties. In this study, a SPME method optimization was conducted using unburned smokeless powders to provide a chemical odor profile assessment. Concurrently, statistical analysis using PCA and Spearman’s rank correlations was performed to explore whether odor volatile composition depicted associations between and within powder brands. The results showed that a longer extraction time (24 h) was optimal across all powders, as this yielded higher compound abundance and number of extracted odor volatiles. The optimal SPME fiber varied per powder, depicting the complexity of powder composition. There were 66 highly frequent compounds among the 18 powders, including 2-ethyl-1-hexanol, diphenylamine (DPA), and dibutyl phthalate. Principal component analysis (PCA) showed that while powders may be of the same type (single/double base), they can still portray clustering differences across and within brands. The Spearman’s rank correlation within powder type suggested that the double-base powders had a slightly higher similarity index when compared with the single-base powder types. Understanding the volatile odor profiles of various smokeless powders can enhance canine training by informing the selection of effective training aids and supporting odor generalization. Full article
Show Figures

Figure 1

28 pages, 8135 KiB  
Article
Drastically Accelerating Fatigue Life Assessment: A Dual-End Multi-Station Spindle Approach for High-Throughput Precision Testing
by Abdurrahman Doğan, Kürşad Göv and İbrahim Göv
Machines 2025, 13(8), 665; https://doi.org/10.3390/machines13080665 - 29 Jul 2025
Viewed by 324
Abstract
This study introduces a time-efficient rotating bending fatigue testing system featuring 11 dual-end spindles, enabling simultaneous testing of 22 specimens. Designed for high-throughput fatigue life (S–N curve) assessment, the system theoretically allows over 93% reduction in total test duration, with 87.5% savings demonstrated [...] Read more.
This study introduces a time-efficient rotating bending fatigue testing system featuring 11 dual-end spindles, enabling simultaneous testing of 22 specimens. Designed for high-throughput fatigue life (S–N curve) assessment, the system theoretically allows over 93% reduction in total test duration, with 87.5% savings demonstrated in validation experiments using AISI 304 stainless steel. The PLC-based architecture provides autonomous operation, real-time failure detection, and automatic cycle logging. ER16 collet holders are easily replaceable within one minute, and all the components are selected from widely available industrial-grade parts to ensure ease of maintenance. The modular design facilitates straightforward adaptation to different station counts. The validation results confirmed an endurance limit of 421 MPa, which is consistent with the established literature and within ±5% deviation. Fractographic analysis revealed distinct crack initiation and propagation zones, supporting the observed fatigue behavior. This high-throughput methodology significantly improves testing efficiency and statistical reliability, offering a practical solution for accelerated fatigue life evaluation in structural, automotive, and aerospace applications. Full article
Show Figures

Figure 1

15 pages, 2636 KiB  
Article
Chest Compression Skill Evaluation System Using Pose Estimation and Web-Based Application
by Ryota Watanabe, Jahidul Islam, Xin Zhu, Emiko Kaneko, Ken Iseki and Lei Jing
Appl. Sci. 2025, 15(15), 8252; https://doi.org/10.3390/app15158252 - 24 Jul 2025
Viewed by 285
Abstract
It is critical to provide life-sustaining treatment to OHCA patients before ambulance care arrives. However, incorrectly performed resuscitation maneuvers reduce the chances of survival and recovery for the victims. Therefore, we must train regularly and learn how to do it correctly. To facilitate [...] Read more.
It is critical to provide life-sustaining treatment to OHCA patients before ambulance care arrives. However, incorrectly performed resuscitation maneuvers reduce the chances of survival and recovery for the victims. Therefore, we must train regularly and learn how to do it correctly. To facilitate regular chest compression training, this study aims to improve the accuracy of a chest compression evaluation system using posture estimation and to develop a web application. To analyze and enhance accuracy, the YOLOv8 posture estimation was used to examine compression depth, recoil, and tempo, and its accuracy was compared to that of the manikin, which has evaluation systems. We conducted comparative experiments with different camera angles and heights to optimize the accuracy of the evaluation. The experimental results showed that an angle of 30 degrees and a height of 50 cm produced superior accuracy. For web application development, a system has been designed to allow users to upload videos for analysis and obtain appropriate compression parameters. The usability evaluation of the application confirmed its ease of use and accessibility, and positive feedback was obtained. In the conclusion, these findings suggest that optimizing recording conditions significantly improves the accuracy of posture-based chest compression evaluation. Future work will focus on enhancing real-time feedback functionality and improving the user interface of the web application. Full article
(This article belongs to the Special Issue Machine Learning in Biomedical Applications)
Show Figures

Figure 1

18 pages, 479 KiB  
Article
Mitigating the Health Impairment Vicious Cycle of Air Traffic Controllers Using Intra-Functional Flexibility: A Mediation-Moderated Model
by Bader Alaydi, Siew-Imm Ng and Xin-jean Lim
Safety 2025, 11(3), 70; https://doi.org/10.3390/safety11030070 - 23 Jul 2025
Viewed by 205
Abstract
Air traffic controllers (ATCOs) make a significant contribution to ensuring flight safety, making this profession a highly stressful job globally. Job demands–resources (JDR) theory proposes a health impairment process stemming from job demand (complexity) to mental workload, which causes job stress, resulting in [...] Read more.
Air traffic controllers (ATCOs) make a significant contribution to ensuring flight safety, making this profession a highly stressful job globally. Job demands–resources (JDR) theory proposes a health impairment process stemming from job demand (complexity) to mental workload, which causes job stress, resulting in compromised flight safety. This vicious cycle is evident among ATCOs and is recognized as an unsustainable management practice. To curb this process, we propose intra-functional flexibility as a conditional factor. Intra-functional flexibility refers to the flexibility in the reallocation and coordination of resources among team members to help in urgent times. This is a relatively new concept and is yet to be empirically tested in the ATCO context. ATCOs work in a dynamic environment filled with sudden surges of urgent jobs to be handled within short time limits. Intra-functional flexibility allows standby crews to be called to ease these tensions when needed. To ascertain the role of intra-functional flexibility in mitigating health impairment among ATCOs, a questionnaire was administered to 324 ATCOs distributed across Saudi Arabia. Partial Least Squares Structural Equation Modeling (PLS-SEM) analysis exhibited two critical findings: First, the study revealed the prevalence of a vicious cycle of health impairment among Saudi ATCOs, whereby job complexity leads to increased mental workload, resulting in elevated levels of job stress. Secondly, the presence of intra-functional flexibility weakened this vicious cycle by mitigating the influence exerted by mental workload on job stress. That is, the mediation-moderated model proposed in this study provides empirical evidence supporting the applicability of intra-functional flexibility in mitigating the dire suffering of ATCOs. This study discusses limitations and future research directions in the end. Full article
Show Figures

Figure 1

16 pages, 2756 KiB  
Article
Development of a Surface-Inset Permanent Magnet Motor for Enhanced Torque Density in Electric Mountain Bikes
by Jun Wei Goh, Shuangchun Xie, Huanzhi Wang, Shengdao Zhu, Kailiang Yu and Christopher H. T. Lee
Energies 2025, 18(14), 3709; https://doi.org/10.3390/en18143709 - 14 Jul 2025
Viewed by 327
Abstract
Electric mountain bikes (eMTBs) demand compact, high-torque motors capable of handling steep terrain and variable load conditions. Surface-mounted permanent magnet synchronous motors (SPMSMs) are widely used in this application due to their simple construction, ease of manufacturing, and cost-effectiveness. However, SPMSMs inherently lack [...] Read more.
Electric mountain bikes (eMTBs) demand compact, high-torque motors capable of handling steep terrain and variable load conditions. Surface-mounted permanent magnet synchronous motors (SPMSMs) are widely used in this application due to their simple construction, ease of manufacturing, and cost-effectiveness. However, SPMSMs inherently lack reluctance torque, limiting their torque density and performance at high speeds. While interior PMSMs (IPMSMs) can overcome this limitation via reluctance torque, they require complex rotor machining and may compromise mechanical robustness. This paper proposes a surface-inset PMSM topology as a compromise between both approaches—introducing reluctance torque while maintaining a structurally simple rotor. The proposed motor features inset magnets shaped with a tapered outer profile, allowing them to remain flush with the rotor surface. This geometric configuration eliminates the need for a retaining sleeve during high-speed operation while also enabling saliency-based torque contribution. A baseline SPMSM design is first analyzed through finite element analysis (FEA) to establish reference performance. Comparative simulations show that the proposed design achieves a 20% increase in peak torque and a 33% reduction in current density. Experimental validation confirms these findings, with the fabricated prototype achieving a torque density of 30.1 kNm/m3. The results demonstrate that reluctance-assisted torque enhancement can be achieved without compromising mechanical simplicity or manufacturability. This study provides a practical pathway for improving motor performance in eMTB systems while retaining the production advantages of surface-mounted designs. The surface-inset approach offers a scalable and cost-effective solution that bridges the gap between conventional SPMSMs and more complex IPMSMs in high-demand e-mobility applications. Full article
Show Figures

Figure 1

14 pages, 1023 KiB  
Article
Economic Impact of Abortions in Dairy Cow Herds
by Osvaldo Palma, Lluís M. Plà-Aragonès, Alejandro Mac Cawley and Víctor M. Albornoz
Vet. Sci. 2025, 12(7), 645; https://doi.org/10.3390/vetsci12070645 - 7 Jul 2025
Viewed by 424
Abstract
This study aimed to explore Markov decision methods in order to solve the problem of dairy cow replacement, adding the special characteristics of two types of abortions due to different sanitary reasons that influence the economic, production, and reproduction performance of these animals. [...] Read more.
This study aimed to explore Markov decision methods in order to solve the problem of dairy cow replacement, adding the special characteristics of two types of abortions due to different sanitary reasons that influence the economic, production, and reproduction performance of these animals. The model was successfully validated against other models published in the literature. Python code v.3.13 was used to solve the problem and to ease future extensions with the inclusion of new variables. The results constitute tools that allow the veterinarian to explore more realistic scenarios by running a Markov simulation model that avoids the complexities leading to the problem of dimensionality in dynamic optimization models. In our study, the economic value of the herd considering RA and NLA abortions shows that the maximum net benefit is USD 178.77 per cow, and non-pregnant cows are slaughtered upon reaching six months of lactation, a value that is within the range of values reported by the literature that we have identified. At the optimum, the replacement model extended with abortion generates a difference of USD 0.69 per cow per month compared to the model that does not include the special abortion features. The changes in the net present value of each cow according to the month of culling depend on the variability of milk income and slaughter value and heifers’ replacement values, suggesting that any measure that seeks to improve the economic benefit of dairy cows should take greater account of these variables. Full article
Show Figures

Figure 1

13 pages, 3092 KiB  
Article
Carbon Dioxide Gas Sensor Based on Terahertz Metasurface with Asymmetric Cross-Shaped Holes Empowered by Quasi-Bound States in the Continuum
by Kai He and Tian Ma
Sensors 2025, 25(13), 4178; https://doi.org/10.3390/s25134178 - 4 Jul 2025
Viewed by 357
Abstract
In this paper, a novel type of polarization-insensitive terahertz metal metasurface with cross-shaped holes is presented, which is designed based on the theory of bound states in continuous media. The fundamental unit of the metasurface comprises a metal tungsten sheet with a cross-shaped [...] Read more.
In this paper, a novel type of polarization-insensitive terahertz metal metasurface with cross-shaped holes is presented, which is designed based on the theory of bound states in continuous media. The fundamental unit of the metasurface comprises a metal tungsten sheet with a cross-shaped hole structure. A thorough analysis of the optical properties and the quasi-BIC response is conducted using the finite element method. Utilizing the symmetry-breaking theory, the symmetry of the metal metasurface is broken, allowing the excitation of double quasi-BIC resonance modes with a high quality factor and high sensitivity to be achieved. Analysis of the multipole power distribution diagram and the spatial distribution of the electric field at the two quasi-BIC resonances verifies that the two quasi-BIC resonances of the metasurface are excited by electric dipoles and electric quadrupoles, respectively. Further simulation analysis demonstrates that the refractive index sensitivities of the two quasi-BIC modes of the metasurface reach 404.5 GHz/RIU and 578.6 GHz/RIU, respectively. Finally, the functional material PHMB is introduced into the metasurface to achieve highly sensitive sensing and detection of CO2 gas concentrations. The proposed metallic metasurface structure exhibits significant advantages, including high sensitivity, ease of preparation, and a high Q-value, which renders it highly promising for a broad range of applications in the domains of terahertz biosensing and highly sensitive gas sensing. Full article
(This article belongs to the Special Issue The Advanced Flexible Electronic Devices: 2nd Edition)
Show Figures

Figure 1

31 pages, 1602 KiB  
Article
Development and TAM-Based Validation of a User Experience Scale for Actual System Use in Online Courses
by Mei Wang, Siva Shankar Ramasamy, Ahmad Yahya Dawod and Xi Yu
Educ. Sci. 2025, 15(7), 855; https://doi.org/10.3390/educsci15070855 - 3 Jul 2025
Viewed by 431
Abstract
This study aims to develop and validate a user experience scale to construct an Actual System Use model for online courses based on the Technology Acceptance Model, allowing for a comprehensive assessment of the multidimensional factors affecting Learning Outcomes and Actual System Use [...] Read more.
This study aims to develop and validate a user experience scale to construct an Actual System Use model for online courses based on the Technology Acceptance Model, allowing for a comprehensive assessment of the multidimensional factors affecting Learning Outcomes and Actual System Use in the context of online courses. The scale includes six core dimensions: Interactive Experience, Content Quality, Learning Outcomes, Teaching Quality, Technical Support, and Learning Motivation. Through a literature review, pre-survey, exploratory factor analysis, and confirmatory factor analysis, the reliability and validity of the developed scale were verified. A second-order complex Structural Equation Model was used to measure users’ Actual System Use with respect to online courses. The results demonstrate that the Interactive Experience and Learning Motivation dimensions play crucial roles in enhancing learners’ engagement and learning satisfaction, while Perceived Usefulness and Perceived Ease of Use significantly influence system usage behaviors. This study provides a systematic theoretical basis and empirical data for the design of online courses, offering valuable insights for optimizing course design and enhancing user experiences. Full article
Show Figures

Figure 1

17 pages, 549 KiB  
Article
Cultural Differences in the Use of Augmented Reality Smart Glasses (ARSGs) Between the U.S. and South Korea: Privacy Concerns and the Technology Acceptance Model
by Se Jung Kim, Yoon Esther Lee and T. Makana Chock
Appl. Sci. 2025, 15(13), 7430; https://doi.org/10.3390/app15137430 - 2 Jul 2025
Viewed by 428
Abstract
Augmented Reality Smart Glasses (ARSGs) allow users to engage in picture-taking and video recording, as well as real-time storage and sharing of pictures and videos through cloud services. Unlike smartphones, newer ARSGs resemble ordinary sunglasses, allowing for unobtrusive recording. As these devices become [...] Read more.
Augmented Reality Smart Glasses (ARSGs) allow users to engage in picture-taking and video recording, as well as real-time storage and sharing of pictures and videos through cloud services. Unlike smartphones, newer ARSGs resemble ordinary sunglasses, allowing for unobtrusive recording. As these devices become available on an international market, it is important to understand how different cultural attitudes towards privacy and the recording and sharing of images of bystanders could impact the acceptance and adoption of ARSGs. South Korea and the United States have vastly different culturally based perceptions of photography and recording in public. S. Korea has cultural and legal restrictions in place, while the U.S.’s values of freedom of expression and individual rights are reflected in limited restrictions. Accordingly, drawing upon the Technology Acceptance Model (TAM), this paper explored the impact of privacy concerns on key constructs of the TAM for U.S. and S. Korean participants. This paper examined how Americans’ (U.S. = 402) and S. Koreans’ (S. Korea = 898) perceived usefulness, perceived ease of use, attitude toward using, and behavioral intention to use ARSGs were impacted by privacy concerns. The results of this study found that S. Korean respondents had significantly greater privacy concerns about using ARSGs than U.S. respondents. However, they also had significantly more positive attitudes and greater behavioral intentions to use ARSGs. Path analyses examining ARSGs’ acceptance revealed that privacy concerns impacted attitudes towards ARSGs, but that these had a greater impact on U.S. participants than on Koreans. The results highlight the importance of considering nuanced cultural perspectives, specifically privacy concerns, in examining the development and adoption of new technologies. Raw data and scripts for this study are available to ensure reproducibility. Full article
(This article belongs to the Special Issue Virtual and Augmented Reality: Theory, Methods, and Applications)
Show Figures

Figure 1

37 pages, 3339 KiB  
Review
Microfluidic Liquid Biopsy Minimally Invasive Cancer Diagnosis by Nano-Plasmonic Label-Free Detection of Extracellular Vesicles: Review
by Keshava Praveena Neriya Hegade, Rama B. Bhat and Muthukumaran Packirisamy
Int. J. Mol. Sci. 2025, 26(13), 6352; https://doi.org/10.3390/ijms26136352 - 1 Jul 2025
Viewed by 647
Abstract
Cancer diagnosis requires alternative techniques that allow for early, non-invasive, or minimally invasive identification. Traditional methods, like tissue biopsies, are highly invasive and can be traumatic for patients. Liquid biopsy, a less invasive option, detects cancer biomarkers in body fluids such as blood [...] Read more.
Cancer diagnosis requires alternative techniques that allow for early, non-invasive, or minimally invasive identification. Traditional methods, like tissue biopsies, are highly invasive and can be traumatic for patients. Liquid biopsy, a less invasive option, detects cancer biomarkers in body fluids such as blood and urine. However, early-stage cancer often presents low biomarker levels, making sensitivity a challenge for integrating liquid biopsy into early diagnosis. Recent studies revealed that extracellular vesicles (EVs) secreted by cells are apt markers for liquid biopsy. Detecting extracellular vesicles (EVs) for liquid biopsy faces challenges like low sensitivity, EV subtype heterogeneity, and difficulty isolating pure populations. Label-free methods, such as plasmonic biosensors and Raman spectroscopy, offer potential solutions by enabling direct analysis without markers, improving accuracy, and reducing complexity. This review paper discusses current challenges in EV-based liquid biopsy for cancer diagnosis and prognosis. It addresses the effective use of microfluidics and nano-plasmonic approaches to address these challenges. Enhancing label-free EV detection in liquid biopsy could revolutionize early cancer diagnosis by offering non-invasive, cost-effective, and rapid testing. This could improve patient outcomes through personalized treatment and ease the burden on healthcare systems. Full article
(This article belongs to the Section Molecular Nanoscience)
Show Figures

Graphical abstract

22 pages, 1595 KiB  
Review
Therapeutic Approaches of Viral Gene Silencing by Small Interfering RNA: Strategies to Prevent the Emergence of Antiviral Resistant Escape Mutants
by Hara Kang, Yun Ji Ga, Jung Won Kim, Chaeyeon Kim, Se-Hwan Son, Chaeeun Gwak and Jung-Yong Yeh
Pharmaceuticals 2025, 18(7), 987; https://doi.org/10.3390/ph18070987 - 1 Jul 2025
Viewed by 506
Abstract
RNA interference (RNAi) was originally regarded as a mechanism of eukaryotic post-transcriptional gene regulation mediated by small interfering RNA (siRNA)-induced sequence-specific RNA degradation. It is well known to exert as an important antiviral defense mechanism in a wide range of organisms, from plants [...] Read more.
RNA interference (RNAi) was originally regarded as a mechanism of eukaryotic post-transcriptional gene regulation mediated by small interfering RNA (siRNA)-induced sequence-specific RNA degradation. It is well known to exert as an important antiviral defense mechanism in a wide range of organisms, from plants to invertebrates. The specificity, ease of design, and ability to target conserved gene regions make siRNA technology a promising approach to combat viral pathogenesis, allowing the targeting of multiple virus strains. The mechanism of sequence complementarity utilized by siRNAs against their targets presents a novel strategy to combat viral infections, as they can specifically target and degrade viral RNA. Consequently, siRNA-based therapeutics have been applied to various viral diseases. This is largely due to the design flexibility and rapid response potential of RNAi technologies, which provide advantages over traditional antiviral agents. However, the emergence of viral escape mutants poses a major barrier to the sustained antiviral activity of siRNA-based therapy. Therefore, devising strategies to overcome the emergence of escape mutants to antiviral siRNAs could enhance the efficacy of siRNA-based therapeutics in providing a rapid response to emerging viral infectious diseases. This review aims to comprehensively summarize the current knowledge on siRNA-based therapeutic approaches against viral infections and elucidate the challenges associated with implementing siRNA treatment, with a specific emphasis on antiviral resistance. Full article
(This article belongs to the Special Issue Design, Synthesis and Development of Novel Antiviral Agents)
Show Figures

Figure 1

18 pages, 1571 KiB  
Article
Genetic Parameters, Linear Associations, and Genome-Wide Association Study for Endotoxin-Induced Cortisol Response in Holstein heifers
by Bruno A. Galindo, Umesh K. Shandilya, Ankita Sharma, Flavio S. Schenkel, Angela Canovas, Bonnie A. Mallard and Niel A. Karrow
Animals 2025, 15(13), 1890; https://doi.org/10.3390/ani15131890 - 26 Jun 2025
Viewed by 325
Abstract
Lipopolysaccharide (LPS) endotoxin is a well-characterized microbe-associated molecular pattern (MAMP) that forms the outer membrane of both pathogenic and commensal Gram-negative bacteria. It plays a crucial role in triggering inflammatory disorders such as mastitis, acidosis, and septicemia. In heifers, an LPS challenge induces [...] Read more.
Lipopolysaccharide (LPS) endotoxin is a well-characterized microbe-associated molecular pattern (MAMP) that forms the outer membrane of both pathogenic and commensal Gram-negative bacteria. It plays a crucial role in triggering inflammatory disorders such as mastitis, acidosis, and septicemia. In heifers, an LPS challenge induces a dynamic stress response, marked by elevated cortisol levels, increased body temperature, and altered immune function. Research indicates that LPS administration leads to a significant rise in cortisol post-challenge. Building on this understanding, the present study aimed to estimate genetic parameters for serum cortisol response to LPS challenge in Holstein heifers and its linear associations with production, health, reproduction, and conformation traits. Additionally, a genome-wide association study (GWAS) was conducted to identify genetic regions associated with cortisol response. A total of 252 animals were evaluated for cortisol response, with correlations estimated between cortisol levels and 55 genomic breeding values for key traits. Genetic parameters and heritability for cortisol response were estimated using Residual Maximum Likelihood (REML) in the Blupf90+ v 2.57 software. Single-Step GWAS (ssGWAS) employing a 10-SNP window approach and 42,123 SNP markers was performed to identify genomic regions that explained at least 0.5% of additive genetic variance. Finally, candidate genes and QTLs located 50 kb up and downstream of those windows were identified. The cortisol response showed significant but weak linear associations with cystic ovaries, body maintenance requirements, lactation persistency, milk yield, and protein yield (p-value ≤ 0.05) and showed suggestive weak linear associations with udder texture, clinical ketosis, heel horn erosion, and milking speed (p-value ≤ 0.15). Cortisol response showed significant additive genetic variance, along with moderate heritability of 0.26 (±0.19). A total of 34 windows explained at least 0.5% of additive genetic variance, and 75 QTLs and 11 candidate genes, comprising the genes CCL20, DAW1, CSMD2, HMGB4, B3GAT2, PARD3, bta-mir-2285aw, CFH, CDH2, ENSBTAG00000052242, and ENSBTAG00000050498, were identified. The functional enrichment analysis allowed us to infer two instances where these gene products could interfere with cortisol production: the first instance is related to the complement system, and the second one is related to the EMT (Epithelium–Mesenchymal Transition) and pituitary gland formation. Among the QTLs, 13 were enriched in the dataset, corresponding to traits related to milk (potassium content), the exterior (udder traits, teat placement, foot angle, rear leg placement, and feet and leg conformation), production (length of productive life, net merit, and type), and reproduction (stillbirth and calving ease). In summary, the cortisol response to LPS challenge in Holstein heifers seems to be moderately heritable and has weak but significant linear associations with important production and health traits. Several candidate genes identified could perform important roles, in at least two ways, for cortisol production, and QTLs were identified close to regions of the genome that explained a significant amount of additive genetic variance for cortisol response. Therefore, further investigations are warranted to validate these findings with a larger dataset. Full article
(This article belongs to the Special Issue Genetic Analysis of Important Traits in Domestic Animals)
Show Figures

Figure 1

Back to TopTop