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17 pages, 13144 KB  
Article
Performance Evaluation of Satellite Observation of Sand/Dust Weather and Its Application in Assessing the Accuracy of Numerical Models
by Pak Wai Chan, Ying Wa Chan, Chun Kit Ho, Yuzhao Ma, Wai Ho Tang, Ho Yi Wong and Xiaoxue Zhang
Appl. Sci. 2025, 15(21), 11745; https://doi.org/10.3390/app152111745 - 4 Nov 2025
Viewed by 187
Abstract
Air quality monitoring and forecasting has been a challenging problem for years. In addition to traditional ground-based observational stations, in recent years there have been more geostationary and polar orbiting satellite observations on air quality. However, evaluation of performance of these observations is [...] Read more.
Air quality monitoring and forecasting has been a challenging problem for years. In addition to traditional ground-based observational stations, in recent years there have been more geostationary and polar orbiting satellite observations on air quality. However, evaluation of performance of these observations is lacking, especially for the region of southern China, which is rarely affected by severe sand/dust weather. In the spring of 2025, two events of sand/dust weather, one case of sand/dust spreading to southern China in April and another case of sand/dust confining to northern China in May, provide a good opportunity for detailed case study and examination of the performance of the tools. The surface particulate matter (PM) concentration retrieved from a geostationary satellite, Geostationary Korea Multi-Purpose Satellite—2B (GEO-KOMPSAT-2B, or GK2B), is studied by checking consistency with the analysis of two numerical models: the Copernicus Atmosphere Monitoring Service model of the European Centre of Medium Range Weather Forecast (ECMWF-CAMS) and Chinese Unified Atmospheric Chemistry Environment model of the China Meteorological Administration (CMA-CUACE). The former shows comparable PM concentration with satellite observations, while overestimation is found with the latter. It is also found that there may be latitude dependence of the quality of the satellite-based data. To further validate the satellite observation data, it is directly compared with the ground-based station measurements in Hong Kong for the event in mid-April 2025, the performance of satellite data points near Hong Kong is generally satisfactory. For polar orbiting satellite, there is information about the aerosol classification in addition to aerosol optical depth, and the classification result is found to be reasonable by comparison with ground-based observation, though some refinements appear to be necessary. The geostationary satellite images provide high spatial coverage and frequently updated air quality data, which are confirmed to be useful in monitoring the southward spread of sand/dust weather to southern China which is a very rare event. The monitoring can be both qualitative and quantitative. The performance of various monitoring and forecasting tools is examined in details based on the cases. It also forms a reference for the use in operation, and opens up a new era for air quality study for southern China. Full article
(This article belongs to the Section Environmental Sciences)
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19 pages, 6409 KB  
Article
Recycling Quarry Dust as a Supplementary Cementitious Material for Cemented Paste Backfill
by Yingying Zhang, Kaifeng Wang, Zhengkun Shi and Shiyu Zhang
Minerals 2025, 15(8), 817; https://doi.org/10.3390/min15080817 - 1 Aug 2025
Viewed by 2582
Abstract
Quarry dust (QD) landfill causes environmental issues that cannot be ignored. In this study, we systematically explore its potential application as a supplementary cementitious material (SCM) in cemented paste backfill (CPB), revealing the activated mechanism of modified QD (MQD) and exploring the hydration [...] Read more.
Quarry dust (QD) landfill causes environmental issues that cannot be ignored. In this study, we systematically explore its potential application as a supplementary cementitious material (SCM) in cemented paste backfill (CPB), revealing the activated mechanism of modified QD (MQD) and exploring the hydration process and workability of CPB containing QD/MQD. The experimental results show that quartz, clinochlore and amphibole components react with CaO to form reactive dicalcium silicate (C2S) and amorphous glass phases, promoting pozzolanic reactivity in MQD. QD promotes early aluminocarbonate (Mc) formation through CaCO3-derived CO32− release but shifts to hemicarboaluminate (Hc) dominance at 28 d. MQD releases active Al3+/Si4+ due to calcination and deconstruction, significantly increasing the amount of ettringite (AFt) in the later stage. With the synergistic effect of coarse–fine particle gradation, MQD-type fresh backfill can achieve a 161 mm flow spread at 20% replacement. Even if this replacement rate reaches 50%, a strength of 19.87 MPa can still be maintained for 28 days. The good workability and low carbon footprint of MQD-type backfill provide theoretical support for—and technical paths toward—QD recycling and the development of low-carbon building materials. Full article
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20 pages, 1118 KB  
Review
Atmospheric Microplastics: Inputs and Outputs
by Christine C. Gaylarde, José Antônio Baptista Neto and Estefan M. da Fonseca
Micro 2025, 5(2), 27; https://doi.org/10.3390/micro5020027 - 30 May 2025
Viewed by 3869
Abstract
The dynamic relationship between microplastics (MPs) in the air and on the Earth’s surface involves both natural and anthropogenic forces. MPs are transported from the ocean to the air by bubble scavenging and sea spray formation and are released from land sources by [...] Read more.
The dynamic relationship between microplastics (MPs) in the air and on the Earth’s surface involves both natural and anthropogenic forces. MPs are transported from the ocean to the air by bubble scavenging and sea spray formation and are released from land sources by air movements and human activities. Up to 8.6 megatons of MPs per year have been estimated to be in air above the oceans. They are distributed by wind, water and fomites and returned to the Earth’s surface via rainfall and passive deposition, but can escape to the stratosphere, where they may exist for months. Anthropogenic sprays, such as paints, agrochemicals, personal care and cosmetic products, and domestic and industrial procedures (e.g., air conditioning, vacuuming and washing, waste disposal, manufacture of plastic-containing objects) add directly to the airborne MP load, which is higher in internal than external air. Atmospheric MPs are less researched than those on land and in water, but, in spite of the major problem of a lack of standard methods for determining MP levels, the clothing industry is commonly considered the main contributor to the external air pool, while furnishing fabrics, artificial ventilation devices and the presence and movement of human beings are the main source of indoor MPs. The majority of airborne plastic particles are fibers and fragments; air currents enable them to reach remote environments, potentially traveling thousands of kilometers through the air, before being deposited in various forms of precipitation (rain, snow or “dust”). The increasing preoccupation of the populace and greater attention being paid to industrial ecology may help to reduce the concentration and spread of MPs and nanoparticles (plastic particles of less than 100 nm) from domestic and industrial activities in the future. Full article
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21 pages, 15399 KB  
Article
Research on the Inversion Method of Dust Content on Mining Area Plant Canopies Based on UAV-Borne VNIR Hyperspectral Data
by Yibo Zhao, Shaogang Lei, Xiaotong Han, Yufan Xu, Jianzhu Li, Yating Duan and Shengya Sun
Drones 2025, 9(4), 256; https://doi.org/10.3390/drones9040256 - 27 Mar 2025
Cited by 1 | Viewed by 608
Abstract
Monitoring dust on plant canopies around open-pit coal mines is crucial to assessing environmental pollution and developing effective dust suppression strategies. This research focuses on the Ha’erwusu open-pit coal mine in Inner Mongolia, China, using measured dust content on plant canopies and UAV-borne [...] Read more.
Monitoring dust on plant canopies around open-pit coal mines is crucial to assessing environmental pollution and developing effective dust suppression strategies. This research focuses on the Ha’erwusu open-pit coal mine in Inner Mongolia, China, using measured dust content on plant canopies and UAV-borne VNIR hyperspectral data as the data sources. The study employed five spectral transformation forms—first derivative (FD), second derivative (SD), logarithm transformation (LT), reciprocal transformation (RT), and square root (SR)—alongside the competitive adaptive reweighted sampling (CARS) method to extract characteristic bands associated with canopy dust. Various regression models, including extreme learning machine (ELM), random forest (RF), partial least squares regression (PLSR), and support vector machine (SVM), were utilized to establish dust inversion models. The spatial distribution of canopy dust was then analyzed. The results demonstrate that the geometric and radiometric correction of the UAV-borne VNIR hyperspectral images successfully restored the true spatial information and spectral features. The spectral transformations significantly enhance the feature information for canopy dust. The CARS algorithm extracted characteristic bands representing 20 to 30% of the total spectral bands, evenly spread across the entire range, thereby reducing the estimation model’s computational complexity. Both feature extraction and model selection influence the inversion accuracy, with the LT-CARS and RF combination offering the best predictive performance. Canopy dust content decreases with increasing distance from the dust source. These findings offer valuable insights for canopy dust retention monitoring and offer a solid foundation for dust pollution management and the development of suppression strategies. Full article
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15 pages, 6196 KB  
Article
Analysis and Control of Abnormal Wear of Reciprocating Compressors in Natural Gas Underground Storage Caverns
by Sijia Zheng, Zhixiang Dai, Fei Wang, Feng Wang, Yongbo Wang, Qin Bie, Wei Jiang, Jingdong Chen, Zicheng Peng and Jie Sun
Processes 2025, 13(4), 996; https://doi.org/10.3390/pr13040996 - 26 Mar 2025
Viewed by 651
Abstract
Throughout China’s 14th five-year plan, the national natural gas pipeline network has been interconnected, and the gas quality became increasingly complex. A certain amount of dust particles widely spread in the natural gas pipeline and lead to abnormal wear of the reciprocating compressor’s [...] Read more.
Throughout China’s 14th five-year plan, the national natural gas pipeline network has been interconnected, and the gas quality became increasingly complex. A certain amount of dust particles widely spread in the natural gas pipeline and lead to abnormal wear of the reciprocating compressor’s compression cylinder within the underground storage cavern. The wear characteristics of the compression cylinder are effectively demonstrated based on the tangential impact energy model, and combined with field measurement and the moving-grid method of computational fluid dynamics. The results reveal that the lubricating oil forms “grinding paste” when mixed with dust particles. With an increase in the dust mass concentration from 0.01% to 3.00%, the viscosity of the “grinding paste” increases from 450,800 mPa·s to 1,274,000 mPa·s, and the density increases from 890 kg/m3 to 980 kg/m3. The abnormal wear frequently occurs at the 12 o’clock and 6 o’clock directions of the compression cylinder. When the piston is in the midpoint of the stroke, the wall shear rate and the wear rate are the highest. When the piston is at both endpoints of the stroke, the wall shear rate and the wear rate are the lowest. For every 1000 h of operation without repairing the cylinder, the dust concentration should be controlled below 0.60%. For every 5000 h and 10,000 h of operation without replacing the cylinder, the dust concentration should be controlled below 1.3% and 0.4%. When the dust mass concentration is 0.01%, the wear rate decreases with decreasing lubricating oil viscosity. When the dust mass concentration is 0.51% and 1.0%, and the lubricating oil viscosities are 259,700 mPa·s, 220,500 mPa·s, and 196,980 mPa·s, the wear rate increases dramatically with decreasing lubricating oil viscosity. Full article
(This article belongs to the Topic Oil and Gas Pipeline Network for Industrial Applications)
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23 pages, 5994 KB  
Article
Three-Dimensional Distribution of Arctic Aerosols Based on CALIOP Data
by Yukun Sun and Liang Chang
Remote Sens. 2025, 17(5), 903; https://doi.org/10.3390/rs17050903 - 4 Mar 2025
Viewed by 1139
Abstract
Tropospheric aerosols play an important role in the notable warming phenomenon and climate change occurring in the Arctic. The accuracy of Cloud–Aerosol Lidar with Orthogonal Polarization (CALIOP) aerosol optical depth (AOD) and the distribution of Arctic AOD based on the CALIOP Level 2 [...] Read more.
Tropospheric aerosols play an important role in the notable warming phenomenon and climate change occurring in the Arctic. The accuracy of Cloud–Aerosol Lidar with Orthogonal Polarization (CALIOP) aerosol optical depth (AOD) and the distribution of Arctic AOD based on the CALIOP Level 2 aerosol products and the Aerosol Robotic Network (AERONET) AOD data during 2006–2021 were analyzed. The distributions, trends, and three-dimensional (3D) structures of the frequency of occurrences (FoOs) of different aerosol subtypes during 2006–2021 are also discussed. We found that the CALIOP AOD exhibited a high level of agreement with AERONET AOD, with a correlation coefficient of approximately 0.67 and an RMSE of less than 0.1. However, CALIOP usually underestimated AOD over the Arctic, especially in wet conditions during the late spring and early summer. Moreover, the Arctic AOD was typically higher in winter than in autumn, summer, and spring. Specifically, polluted dust (PD), dust, and clean marine (CM) were the dominant aerosol types in spring, autumn, and winter, while in summer, ES (elevated smoke) from frequent wildfires reached the highest FoOs. There were increasing trends in the FoOs of CM and dust, with decreasing trends in the FoOs of PD, PC (polluted continental), and DM (dusty marine) due to Arctic amplification. In general, the vertical distribution patterns of different aerosol types showed little seasonal variation, but their horizontal distribution patterns at various altitudes varied by season. Furthermore, locally sourced aerosols such as dust in Greenland, PD in eastern Siberia, and ES in middle Siberia can spread to surrounding areas and accumulate further north, affecting a broader region in the Arctic. Full article
(This article belongs to the Section Atmospheric Remote Sensing)
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15 pages, 7479 KB  
Article
Numerical Simulation Study of Gas–Liquid–Solid Triphase Coupling in Fully Mechanized Excavation Faces with Variation in Dust Source Points
by Jianguo Wang, Bolan Wang and Jinmeng Gai
Sustainability 2024, 16(19), 8523; https://doi.org/10.3390/su16198523 - 30 Sep 2024
Cited by 1 | Viewed by 1420
Abstract
In view of the current situation where research on the dust diffusion laws of different dust source points is limited and the gap with the actual field situation is too large; this study employs an innovative gas–liquid–solid triphase coupling method to investigate how [...] Read more.
In view of the current situation where research on the dust diffusion laws of different dust source points is limited and the gap with the actual field situation is too large; this study employs an innovative gas–liquid–solid triphase coupling method to investigate how dust moves and spreads in the fully mechanized excavation face 431305 at the Liangshuijing Mine; focusing on both the dust field and the dust–fog coupled field. The results indicate that using the long-pressure short-suction ventilation method; dust movement in the roadway is primarily influenced by the airflow; which can be classified into vortex; jet; and return flow regions. The analysis reveals that different dust source points affect dust distribution patterns. Dust source 1 generates the highest dust concentration; primarily accumulating on the duct side and return air side of the roadway. By contrast; dust source 2’s dust mainly gathers at the heading and the front of the cutting head. Dust sources 3 and 4 show lower dust concentrations near the top of the roadway. Dust source 5 achieves the most effective dust removal; aided by airflow and a suction fan; showcasing superior dust performance. A comprehensive comparison indicates that dust source 1 has the highest overall dust concentration. Therefore; further simulation of the distribution law of dust generated at dust source 1 under the action of water mist reveals that the dust concentration near the heading face is reduced from 2000 mg/m3 under the action of single air flow to about 1100 mg/m3. At t = 5 s; the spray droplets almost cover the entire tunneling face; leading to a significant decrease in dust concentration within 10–25 m from the tunneling face. Within 40 s; both coal dust and spray droplets are significantly reduced. The field measurement results verify the accuracy of the simulation results and provide certain guidance for promoting the sustainable development of the coal industry. Full article
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13 pages, 5522 KB  
Article
Managemental Impacts on Particulate Matter Emission in Tunnel-Ventilated Broiler Houses
by Hyo-Jae Seo, Il-Hwan Seo and Byung-Wook Oh
Agriculture 2024, 14(2), 204; https://doi.org/10.3390/agriculture14020204 - 27 Jan 2024
Cited by 3 | Viewed by 1831
Abstract
In livestock houses, particulate matter (PM) is a critical factor not only for disease and odor spread but also for the work environment. In particular, workers are exposed to high concentrations of organic particulate matter and harmful gases while performing their tasks, and, [...] Read more.
In livestock houses, particulate matter (PM) is a critical factor not only for disease and odor spread but also for the work environment. In particular, workers are exposed to high concentrations of organic particulate matter and harmful gases while performing their tasks, and, as they age, they become more vulnerable to respiratory diseases. This study analyzed the PM concentrations in commercial broiler houses, focusing on the differences in ventilation rates according to the season and the type of work, categorized into a static work period (SWP) and dynamic work period (DWP). In the regional monitoring using gravimetric methods, the average PM concentrations were found to be within acceptable limits, with TSP at 1042 µg/m3, PM-10 at 718 µg/m3, and PM-2.5 at 137 µg/m3. These values did not exceed the recommended exposure limits for inhalable dust at 2400 µg/m3 and respirable dust at 160 µg/m3 in chicken farmers. However, in the personal monitoring using a real-time aerosol spectrometer, it was revealed that the aerosol concentrations during DWP exceeded the standards by up to 214%. Specifically, during DWP, the concentrations were 1.74 times higher for TSP, 1.40 times higher for PM-10, and 1.22 times higher for PM-2.5 compared to SWP. It was observed that during the movement of workers, the physical generation of particles around 10 µm, such as feed and bedding, occurred due to the movement of chickens, which influenced the aerosol concentration. Full article
(This article belongs to the Section Farm Animal Production)
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18 pages, 6141 KB  
Article
A Forest Fire Recognition Method Based on Modified Deep CNN Model
by Shaoxiong Zheng, Xiangjun Zou, Peng Gao, Qin Zhang, Fei Hu, Yufei Zhou, Zepeng Wu, Weixing Wang and Shihong Chen
Forests 2024, 15(1), 111; https://doi.org/10.3390/f15010111 - 5 Jan 2024
Cited by 21 | Viewed by 3786
Abstract
Controlling and extinguishing spreading forest fires is a challenging task that often leads to irreversible losses. Moreover, large-scale forest fires generate smoke and dust, causing environmental pollution and posing potential threats to human life. In this study, we introduce a modified deep convolutional [...] Read more.
Controlling and extinguishing spreading forest fires is a challenging task that often leads to irreversible losses. Moreover, large-scale forest fires generate smoke and dust, causing environmental pollution and posing potential threats to human life. In this study, we introduce a modified deep convolutional neural network model (MDCNN) designed for the recognition and localization of fire in video imagery, employing a deep learning-based recognition approach. We apply transfer learning to refine the model and adapt it for the specific task of fire image recognition. To combat the issue of imprecise detection of flame characteristics, which are prone to misidentification, we integrate a deep CNN with an original feature fusion algorithm. We compile a diverse set of fire and non-fire scenarios to construct a training dataset of flame images, which is then employed to calibrate the model for enhanced flame detection accuracy. The proposed MDCNN model demonstrates a low false alarm rate of 0.563%, a false positive rate of 12.7%, a false negative rate of 5.3%, and a recall rate of 95.4%, and achieves an overall accuracy of 95.8%. The experimental results demonstrate that this method significantly improves the accuracy of flame recognition. The achieved recognition results indicate the model’s strong generalization ability. Full article
(This article belongs to the Section Natural Hazards and Risk Management)
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17 pages, 2372 KB  
Article
Advancing Sustainability: Effective Strategies for Carbon Footprint Reduction in Seaports across the Colombian Caribbean
by Liliana Centanaro Acuña, Esteban Ochoa Caceres, Brayan Caballero Campo, Edson Campanhola Bortoluzzi, Alcindo Neckel, Andrea Liliana Moreno-Ríos, Leila Dal Moro, Marcos L. S. Oliveira, Giana de Vargas Mores and Claudete Gindri Ramos
Sustainability 2023, 15(22), 15819; https://doi.org/10.3390/su152215819 - 10 Nov 2023
Cited by 4 | Viewed by 2626
Abstract
Colombian port terminals in the Caribbean are being called upon to increase the sustainability of their operations to better fit with the environmental dynamics of their locations. Within this context, the Palermo Sociedad Portuaria (PSP) has taken a proactive stance in identifying the [...] Read more.
Colombian port terminals in the Caribbean are being called upon to increase the sustainability of their operations to better fit with the environmental dynamics of their locations. Within this context, the Palermo Sociedad Portuaria (PSP) has taken a proactive stance in identifying the factors contributing to its CO2 emissions. This study evaluated the CO2 emissions of the PSP in 2019 and 2020 and, through the implementation of sustainable practices (rock dust spreading, composting and reducing the burning of fossil fuels), examined the mitigation of the port’s carbon footprint (CF) in the year 2022. Based on collaborative management results and efforts, a set of viable mitigation strategies adapted to port operations was formulated. Viability was assessed through monitoring of the practical implementations encompassing initiatives such as fuel reduction, waste composting and the application of rock dust. The introduction of the CARE system in the operational equipment led to a reduction in fuel consumption over five periods—amounting to an overall emission decrease of 1629 metric tons of CO2 equivalent (ton CO2 eq). Meanwhile, the strategic composting of waste generated by port activities (including organic waste, hand towels, coffee grounds and landscaping waste) resulted in the potential reduction of 2 metric tons of CO2 annually. The application of rock dust (10 kg m−2) in the available green spaces within the operational areas contributed to a decrease of 0.00080543 ton CO2 eq over 45 days. The implementation of these three key measures over the course of a year has the potential to prevent the release of 37 ton CO2 eq, signifying a 2% decrease in overall CF when compared to the base year of 2020. This investigation was rooted in the current operational reality of the port terminal and its correlated activities. The strategies deployed underscore the feasibility of low-cost solutions that can be emulated across port terminals in pursuit of the holistic aspirations encapsulated in the concepts of a “green port” and a “smart port”. Full article
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15 pages, 3198 KB  
Article
Sustainable Aspects of Multiple-Use Woven Fabric in the Hospital Environment: Comfort and Textile Dust Generation Perspectives
by Ana Palčić, Sandra Flinčec Grgac and Snježana Brnada
Sustainability 2023, 15(21), 15364; https://doi.org/10.3390/su152115364 - 27 Oct 2023
Viewed by 1647
Abstract
Textile dust released from hospital textiles is a considerable food source for pathogenic microorganisms and can lead to infections and illness in patients and medical staff. In addition, it often causes malfunctions in sophisticated medical equipment. The structural parameters of the fabric, such [...] Read more.
Textile dust released from hospital textiles is a considerable food source for pathogenic microorganisms and can lead to infections and illness in patients and medical staff. In addition, it often causes malfunctions in sophisticated medical equipment. The structural parameters of the fabric, such as the raw material composition, the thread density and the fabric weave, can influence the amount of dust produced. Friction between threads in a woven fabric plays a crucial role in dust generation, and friction is influenced by the surface structure of fibres, yarns and fabric. Understanding these factors can help in the development of fabrics with lower release of textile dust, which can reduce the risk of spreading infections in healthcare facilities. In this paper, the influence of the washing cycle on the change in morphological properties of fabrics in satin weave made of cotton–polyester blends was investigated. The study showed that as the number of maintenance washing cycles increases, the waviness, roughness and average amplitude of the surface roughness profile of the wove fabrics increases. Damage to the fibres during washing results in dust release, with synthetic fibres releasing less dust than cotton fibres. These results provide important information about the change in fabric properties during the washing process, which may be useful for further research and development of materials for use in a hospital environment. Full article
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15 pages, 4295 KB  
Article
Explosion Risk of Metal Powder Used in Additive Manufacturing
by Siheng Sun, Chonglin Xing, Jianxu Ding, Yan Cheng, Kai Liu and Lei Pang
Processes 2023, 11(10), 2943; https://doi.org/10.3390/pr11102943 - 10 Oct 2023
Cited by 4 | Viewed by 4396
Abstract
The explosive characteristic parameters of additive manufacturing (AM) dust were systematically studied. A systematic experimental study was performed on the characteristic parameters of dust explosions fueled by metal powder for AM, to obtain the distribution pattern of those parameters for four types of [...] Read more.
The explosive characteristic parameters of additive manufacturing (AM) dust were systematically studied. A systematic experimental study was performed on the characteristic parameters of dust explosions fueled by metal powder for AM, to obtain the distribution pattern of those parameters for four types of AM dust samples. Based on experimental data, the dust-explosive severity was classified via two-dimensional matrix analysis in terms of Pmax and Kst, and the dust-explosive sensitivity was classified via three-dimensional matrix analyses of the MIE, MIT, and MEC. Finally, a dust-explosive risk assessment model of AM metal powder was established based on comprehensive considerations of the dust-explosive severity and sensitivity, and four types of AM dust sample were applied empirically. The risk assessment method can provide a scientifically credible and effective basis for assessing and improving the AM process based on powder spreading. Full article
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14 pages, 9008 KB  
Article
Monodisperse Fluorescent Polystyrene Microspheres for Staphylococcus aureus Aerosol Simulation
by Siyu Lu, Fan Li, Bo Liu, Kun Yang, Feng Tian, Zhi Cheng, Sheng Ding and Kexin Hou
Polymers 2023, 15(17), 3614; https://doi.org/10.3390/polym15173614 - 31 Aug 2023
Cited by 5 | Viewed by 2782
Abstract
Staphylococcus aureus (SA) is one of the most common causes of hospital-acquired infections and foodborne illnesses and is commonly found in nature in air, dust, and water. The spread and transmission of SA aerosols in the air has the potential to cause epidemic [...] Read more.
Staphylococcus aureus (SA) is one of the most common causes of hospital-acquired infections and foodborne illnesses and is commonly found in nature in air, dust, and water. The spread and transmission of SA aerosols in the air has the potential to cause epidemic transmission among humans and between humans and animals. To effectively provide the timely warning of SA aerosols in the atmosphere, the identification and detection of SA aerosol concentrations are required. Due to their homogeneous physicochemical properties, highly monodisperse submicron polystyrene (PS) microspheres can be used as one of the simulants of SA aerosols. In this study, 800 nm monodisperse fluorescent PS (f-PS) microspheres with fluorescence spectra and particle size distribution similar to those of SA were prepared. The 800 nm monodisperse f-PS microspheres had a fluorescence characteristic peak at 465 nm; in aerosols, 800 nm monodisperse f-PS microspheres with a similar particle size distribution to that of SA were further verified, mainly in the range of 500 nm–1000 nm; finally, it was found that the f-PS microspheres still possessed similar fluorescence characteristics after 180 days. The f-PS microspheres prepared in this study are very close to SA in terms of particle size and fluorescence properties, providing a new idea for aerosol analogs of SA. Full article
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9 pages, 3563 KB  
Article
Preclinical Studies on the Safety and Toxicity of Photoditazine in the Antibacterial Photodynamic Therapy of Uropathogenic Bacteria
by Olga Streltsova, Artem Antonyan, Nadezhda Ignatova, Katerina Yunusova, Vadim Elagin and Vladislav Kamensky
Biomedicines 2023, 11(8), 2283; https://doi.org/10.3390/biomedicines11082283 - 17 Aug 2023
Cited by 1 | Viewed by 1772
Abstract
The ‘dusting’ technique of lithotripsy for the removal of infected urinary calculi and the wide use of drainage after endoscopic surgery may stimulate spreading of multidrug-resistant bacterial strains. Antibacterial photodynamic therapy (PDT) is one promising method for the elimination these strains. The purpose [...] Read more.
The ‘dusting’ technique of lithotripsy for the removal of infected urinary calculi and the wide use of drainage after endoscopic surgery may stimulate spreading of multidrug-resistant bacterial strains. Antibacterial photodynamic therapy (PDT) is one promising method for the elimination these strains. The purpose of our study was to evaluate alterations of renal pelvis morphology and renal function in laboratory animals after bactericidal regimens of PDT. Renal pelvises of pigs were filled with Photoditazine and then assessed either by examining the accumulation of Photoditazine in the urothelium or by illumination with a laser at a wavelength of 662 nm. A renal test and a complete blood count was performed to assess a negative effect of the treatment on health. Structural alterations of the kidney tissues were analyzed by histological examination. No photosensitizer fluorescence was detected in the urothelium of the pelvis. Histological study showed that PDT caused minor changes to the urothelium of the renal pelvis but did not affect the underlying connective tissue. No renal function abnormalities were found after PDT. Thus, the study indicates that antibacterial PDT is a safety technique that can complement common antibiotic therapy in the surgical treatment of urolithiasis. Full article
(This article belongs to the Special Issue Drug-Resistant Bacterial Infections and Alternative Therapies)
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11 pages, 4258 KB  
Article
Sampling and Characterization of Bioaerosols in Poultry Houses
by Brooke L. Smith and Maria D. King
Microorganisms 2023, 11(8), 2068; https://doi.org/10.3390/microorganisms11082068 - 11 Aug 2023
Cited by 5 | Viewed by 2116
Abstract
Two poultry Confined Animal Feeding Units (CAFUs), “House A” and “House B”, were selected from the TAMU poultry facility for the study, and samples were collected over a five-day period. Bioaerosol sampling was conducted using a Wetted Wall Cyclone (WWC) bioaerosol collector at [...] Read more.
Two poultry Confined Animal Feeding Units (CAFUs), “House A” and “House B”, were selected from the TAMU poultry facility for the study, and samples were collected over a five-day period. Bioaerosol sampling was conducted using a Wetted Wall Cyclone (WWC) bioaerosol collector at the two CAFU houses, in which House A housed approximately 720 broiler chickens and roosters, while House B remained unoccupied and served as a reference. Both houses consisted of 24 pens arranged on either side of a central walkway. Bacterial content analysis was conducted using microbial plating, real-time Polymerase Chain Reaction (PCR), and Fatty Acid Methyl Ester (FAME) analysis, while ambient temperature and relative humidity were also monitored. The concentrations of microorganisms in House A showed a highly dynamic range, ranging from 4000 to 60,000 colony forming units (CFU) per cubic meter of air. Second, the WWC samples contained approximately ten-fold more bacterial DNA than the filter samples, suggesting higher levels of viable cells captured by the WWC. Third, significant concentrations of pathogens, including Salmonella, Staphylococcus, and Campylobacter, were detected in the poultry facility. Lastly, the WWC system demonstrated effective functionality and continuous operation, even in the challenging sampling environment of the CAFU. The goal of this study was to characterize the resident population of microorganisms (pathogenic and non-pathogenic) present in the CAFUs and to evaluate the WWC’s performance in such an environment characterized by elevated temperature, high dust content, and feathers. This knowledge could then be used to improve understanding microorganism dynamics in CAFUs including the spread of bacterial infections between animals and from animals to humans that work in these facilities, as well as of the WWC performance in this type of environment (elevated temperature, high content of dust and feathers). A more comprehensive understanding can aid in improving the management of bacterial infections in these settings. Full article
(This article belongs to the Special Issue Bacterial and Antibiotic Resistance in the Environment)
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