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14 pages, 7190 KB  
Article
Chaos Prediction and Nonlinear Dynamic Analysis of a Dimple-Equipped Electrostatically Excited Microbeam
by Ayman M. Alneamy
Mathematics 2025, 13(18), 2925; https://doi.org/10.3390/math13182925 - 10 Sep 2025
Viewed by 355
Abstract
As MEMS design encounters growing challenges, particularly stiction between movable and stationary electrodes, dielectric charging, pull-in instability, and multi-valued response characteristics, the integration of dimple-equipped structures has emerged as a pivotal solution to mitigate these fundamental issues. Consequently, this study investigates the dynamic [...] Read more.
As MEMS design encounters growing challenges, particularly stiction between movable and stationary electrodes, dielectric charging, pull-in instability, and multi-valued response characteristics, the integration of dimple-equipped structures has emerged as a pivotal solution to mitigate these fundamental issues. Consequently, this study investigates the dynamic behavior of an electrostatically actuated double-clamped microbeam incorporating dimples and contact pads. While the dimples enhance the beam’s travel range, they may also induce an impact mode upon contact with the landing pads, leading to complex nonlinear dynamic phenomena. A reduced-order model was developed to numerically solve the governing equation of motion. The microbeam’s response was analyzed both with and without dimples using multiple analytical techniques, including bifurcation diagrams and discrete excitation procedures near the impacting regime. The findings demonstrate that the inclusion of dimples effectively suppresses stiction, pull-in instability, and multi-valued responses. The results indicate that upon contacting the landing pads, the beam exhibits pronounced nonlinear dynamic behaviors, manifesting as higher-period oscillations such as period-3, period-4 and period-5 and then fully developed chaotic attractors. Indeed, this specifically demonstrates the potential of using the dynamic transition from a steady-state to a chaotic response to build novel MEMS sensors. Full article
(This article belongs to the Special Issue Advances in Nonlinear Analysis: Theory, Methods and Applications)
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23 pages, 4352 KB  
Article
Quantifying Inter-Ply Friction and Clamping Effects via an Experimental–Numerical Framework: Advancing Non-Coherent Deformation Control of Uncured Metal–Fiber-Reinforced Polymer Laminates
by Yunlong Chen and Shichen Liu
Polymers 2025, 17(17), 2330; https://doi.org/10.3390/polym17172330 - 28 Aug 2025
Viewed by 1332
Abstract
Pre-stacked uncured metal–fiber-reinforced polymer (FRP) laminates, which are critical for aerospace components like double-curved fuselage panels, wing ribs, and engine nacelles, exhibit better deformation behavior than their fully cured counterparts. However, accurate process simulation requires precise material characterization and process optimization to achieve [...] Read more.
Pre-stacked uncured metal–fiber-reinforced polymer (FRP) laminates, which are critical for aerospace components like double-curved fuselage panels, wing ribs, and engine nacelles, exhibit better deformation behavior than their fully cured counterparts. However, accurate process simulation requires precise material characterization and process optimization to achieve a defect-free structural design. This study focuses on two core material behaviors of uncured laminates—inter-ply friction at metal–prepreg interfaces and out-of-plane bending—and optimizes process parameters for their non-coherent deformation. Experimental tests included double-lap sliding tests (to quantify inter-ply friction) and clamped-beam bending tests (to characterize out-of-plane bending); a double-curved dome part was designed to assess the effects of the material constituent, fiber orientation, inter-ply friction, and clamping force, with validation via finite element modeling (FEM) in Abaqus software. The results indicate that the static–kinetic friction model effectively predicts inter-ply friction behavior, with numerical friction coefficient–displacement trends closely matching experimental data. Additionally, the flexural bending model showed that greater plastic deformation in metal layers increased bending force while reducing post-unloading spring-back depth. Furthermore, for non-coherent deformation, higher clamping forces improve FRP prepreg deformation and mitigate buckling, but excessive plastic deformation raises metal cracking risk. This work helps establish a combined experimental–numerical framework for the defect prediction and process optimization of complex lightweight components, which address the core needs of modern aerospace manufacturing. Full article
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21 pages, 5059 KB  
Article
Experimental and Numerical Validation of an Extended FFR Model for Out-of-Plane Vibrations in Discontinuous Flexible Structures
by Sherif M. Koda, Masami Matsubara, Ahmed M. R. Fath El-Bab and Ayman A. Nada
Appl. Syst. Innov. 2025, 8(5), 118; https://doi.org/10.3390/asi8050118 - 22 Aug 2025
Viewed by 644
Abstract
Toward the innovative design of tunable structures for energy generation, this paper presents an extended Floating Frame of Reference (FFR) formulation capable of modeling slope discontinuities in flexible multibody systems—overcoming a key limitation of conventional FFR methods that assume slope continuity. The model [...] Read more.
Toward the innovative design of tunable structures for energy generation, this paper presents an extended Floating Frame of Reference (FFR) formulation capable of modeling slope discontinuities in flexible multibody systems—overcoming a key limitation of conventional FFR methods that assume slope continuity. The model is validated using a spatial double-pendulum structure composed of circular beam elements, representative of out-of-plane energy harvesting systems. To investigate the influence of boundary constraints on dynamic behavior, three electromagnetic clamping configurations—Fixed–Free–Free (XFF), Fixed–Free–Fixed (XFX), and Free–Fixed–Free (FXF)—are implemented. Tri-axial accelerometer measurements are analyzed via Fast Fourier Transform (FFT), revealing natural frequencies spanning from 38.87 Hz (lower frequency range) to 149.01 Hz (higher frequency range). For the lower frequency range, the FFR results (38.76 Hz) show a close match with the experimental prediction (38.87 Hz) and ANSYS simulation (36.49 Hz), yielding 0.28% error between FFR and experiments and 5.85% between FFR and ANSYS. For the higher frequency range, the FFR model (148.17 Hz) achieves 0.56% error with experiments (149.01 Hz) and 0.85% with ANSYS (146.91 Hz). These high correlation percentages validate the robustness and accuracy of the proposed FFR formulation. The study further shows that altering boundary conditions enables effective frequency tuning in discontinuous structures—an essential feature for the optimization of application-specific systems such as wave energy converters. This validated framework offers a versatile and reliable tool for the design of vibration-sensitive devices with geometric discontinuities. Full article
(This article belongs to the Section Control and Systems Engineering)
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18 pages, 7292 KB  
Article
Optimization of Acceleration and Driving Force for Double-Toggle Stephenson-Chain Mold Clamping Mechanisms
by Tzu-Hsia Chen and Po-Cheng Lai
Appl. Sci. 2025, 15(15), 8463; https://doi.org/10.3390/app15158463 - 30 Jul 2025
Viewed by 380
Abstract
The mold clamping mechanism is crucial in injection molding machines and significantly influences molding. This research optimizes the Stephenson-chain mechanism with double-toggle effects, particularly focusing on acceleration and driving force. A design incorporating double-toggle effects in the closed position enhances clamping force and [...] Read more.
The mold clamping mechanism is crucial in injection molding machines and significantly influences molding. This research optimizes the Stephenson-chain mechanism with double-toggle effects, particularly focusing on acceleration and driving force. A design incorporating double-toggle effects in the closed position enhances clamping force and ensures safety. For a 6-bar linkage, the Watt-chain mechanism and Stephenson-chain mechanism are available. In this paper, Stephenson-chain mechanisms were selected and subjected to a comprehensive analysis of their kinematic characteristics using vector loop and finite difference methods. The optimal design process included defining the objective function and evaluating the maximum acceleration and force ratio. The results show that the optimal Stephenson-I mechanism achieves a 1.92% increase in the maximum acceleration, and the maximum driving force decreases by 12.34% compared to the optimal Watt-chain mechanism. The Stephenson-II mechanism performs even better, with a 33.94% reduction in maximum acceleration and a 6.81% decrease in maximum driving force compared to the optimal Watt-chain mechanism. The results indicate that the Stephenson-II mechanism outperforms the Stephenson-I mechanism and other existing designs in terms of the maximum acceleration and driving force. Full article
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18 pages, 3197 KB  
Article
The Progressive Damage Modeling of Composite–Steel Lapped Joints
by Alaa El-Sisi, Ahmed Elbelbisi, Ahmed Elkilani and Hani Salim
J. Compos. Sci. 2025, 9(7), 350; https://doi.org/10.3390/jcs9070350 - 7 Jul 2025
Viewed by 1026
Abstract
In advanced structural applications—aerospace and automotive—fiber-laminated composite (FRP) materials are increasingly used for their superior strength-to-weight ratios, making the reliability of their mechanical joints a critical concern. Mechanically fastened joints play a major role in ensuring the structural stability of FRP Composite structures; [...] Read more.
In advanced structural applications—aerospace and automotive—fiber-laminated composite (FRP) materials are increasingly used for their superior strength-to-weight ratios, making the reliability of their mechanical joints a critical concern. Mechanically fastened joints play a major role in ensuring the structural stability of FRP Composite structures; however, accurately predicting their failure behavior remains a major challenge due to the anisotropic and heterogeneous nature of composite materials. This paper presents a progressive damage modeling approach to investigate the failure modes and joint strength of mechanically fastened carbon fiber-laminated (CFRP) composite joints. A 3D constitutive model based on continuum damage mechanics was developed and implemented within a three-dimensional finite element framework. The joint model comprises a composite plate, a steel plate, a steel washer, and steel bolts, capturing realistic assembly behavior. Both single- and double-lap joint configurations, featuring single and double bolts, were analyzed under tensile loading. The influence of clamping force on joint strength was also investigated. Model predictions were validated against existing experimental results, showing a good correlation. It was observed that double-lap joints exhibit nearly twice the strength of single-lap joints and can retain up to 85% of the strength of a plate with a hole. Furthermore, double-lap configurations support higher clamping forces, enhancing frictional resistance at the interface and load transfer efficiency. However, the clamping force must be optimized, as excessive values can induce premature damage in the composite before external loading. The stiffness of double-bolt double-lap (3DD) joints was found to be approximately three times that of single-bolt single-lap (3DS) joints, primarily due to reduced rotational flexibility. These findings provide useful insights into the design and optimization of composite bolted joints under tensile loading. Full article
(This article belongs to the Special Issue Characterization and Modelling of Composites, Volume III)
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28 pages, 9836 KB  
Article
Cascaded H-Bridge Multilevel Converter Topology for a PV Connected to a Medium-Voltage Grid
by Hammad Alnuman, Essam Hussain, Mokhtar Aly, Emad M. Ahmed and Ahmed Alshahir
Machines 2025, 13(7), 540; https://doi.org/10.3390/machines13070540 - 21 Jun 2025
Viewed by 964
Abstract
When connecting a renewable energy source to a medium-voltage grid, it has to fulfil grid codes and be able to work in a medium-voltage range (>10 kV). Multilevel converters (MLCs) are recognized for their low total harmonic distortion (THD) and ability to work [...] Read more.
When connecting a renewable energy source to a medium-voltage grid, it has to fulfil grid codes and be able to work in a medium-voltage range (>10 kV). Multilevel converters (MLCs) are recognized for their low total harmonic distortion (THD) and ability to work at high voltage compared to other converter types, making them ideal for applications connected to medium-voltage grids whilst being compliant with grid codes and voltage ratings. Cascaded H-bridge multilevel converters (CHBs-MLC) are a type of MLC topology, and they does not need any capacitors or diodes for clamping like other MLC topologies. One of the problems in these types of converters involves the double-frequency harmonics in the DC linking voltage and power, which can increase the size of the capacitors and converters. The use of line frequency transformers for isolation is another factor that increases the system’s size. This paper proposes an isolated CHBs-MLC topology that effectively overcomes double-line frequency harmonics and offers isolation. In the proposed topology, each DC source (renewable energy source) supplies a three-phase load rather than a single-phase load that is seen in conventional MLCs. This is achieved by employing a multi-winding high-frequency transformer (HFT). The primary winding consists of a winding connected to the DC sources. The secondary windings consist of three windings, each supplying one phase of the load. This configuration reduces the DC voltage link ripples, thus improving the power quality. Photovoltaic (PV) renewable energy sources are considered as the DC sources. A case study of a 1.0 MW and 13.8 kV photovoltaic (PV) system is presented, considering two scenarios: variations in solar irradiation and 25% partial panel shedding. The simulations and design results show the benefits of the proposed topology, including a seven-fold reduction in capacitor volume, a 2.7-fold reduction in transformer core volume, a 50% decrease in the current THD, and a 30% reduction in the voltage THD compared to conventional MLCs. The main challenge of the proposed topology is the use of more switches compared to conventional MLCs. However, with advancing technology, the cost is expected to decrease over time. Full article
(This article belongs to the Special Issue Power Converters: Topology, Control, Reliability, and Applications)
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17 pages, 2482 KB  
Article
Heterosynaptic Regulation of α2A-Adrenoceptors on Glutamate/GABA Release in the Prefrontal Cortex of Rats
by Yaru Wei, Yuhan Jiao, Xiaoting He, Xiaodong Tao, Baoming Li and Xuehan Zhang
Biomedicines 2025, 13(6), 1322; https://doi.org/10.3390/biomedicines13061322 - 28 May 2025
Viewed by 859
Abstract
Background/Objectives: Norepinephrine (NE) plays a crucial role in modulating cognitive processes via α2A-adrenoceptors (α2A-ARs) within the prefrontal cortex (PFC), an essential brain region responsible for higher cognitive functions. The α2A-ARs are found on both postsynaptic and presynaptic membranes in the PFC. Previous studies [...] Read more.
Background/Objectives: Norepinephrine (NE) plays a crucial role in modulating cognitive processes via α2A-adrenoceptors (α2A-ARs) within the prefrontal cortex (PFC), an essential brain region responsible for higher cognitive functions. The α2A-ARs are found on both postsynaptic and presynaptic membranes in the PFC. Previous studies have shown that presynaptic α2A-ARs, predominantly located at NE terminals, function as autoreceptors that inhibit NE release. However, the expression of α2A-ARs at non-NE terminals, such as glutamate and GABA, remains ambiguous. To clarify the expression patterns and potential roles of α2A-ARs at non-NE terminals, we investigated their presence at the axon terminals of excitatory glutamate neurons and inhibitory GABA neurons in the rat PFC using immunofluorescence double-labeling, whole-cell patch-clamp recordings, and pharmacological approaches. Methods: To clarify the expression patterns and potential roles of α2A-ARs at non-NE terminals, we investigated their presence at the axon terminals of glutamate neurons and GABA neurons in the rat PFC using immunofluorescence double-labeling, whole-cell patch-clamp recordings, and pharmacological approaches. Results: Our findings delineated the distribution of α2A-ARs at the axon terminals of both glutamate and GABA neurons, and the expression of α2A-AR in the pyramidal neurons within the rat PFC as well. Furthermore, we employed the selective α2A-AR agonist guanfacine to assess the functional role of presynaptic α2A-ARs at these non-NE terminals. Following the application of the PKA inhibitor PKI5–24 to block postsynaptic α2A-AR function, guanfacine still significantly decreased the frequency (not the amplitude) of miniature excitatory postsynaptic currents (mEPSCs) and miniature inhibitory postsynaptic currents (mIPSCs) in layer 5–6 pyramidal neurons. Notably, the frequency reduction induced by guanfacine persisted even after the depletion of presynaptic NE vesicles. Conclusions: These findings offer a comprehensive analysis of presynaptic α2A-AR expression and function in the PFC, revealing for the first time their role as heteroreceptors that modulate the release of glutamate and GABA. Our results provide morphological and electrophysiological insights into a potential mechanism through which α2A-AR stimulation enhances cognitive functions. Full article
(This article belongs to the Special Issue Synaptic Function and Modulation in Health and Disease)
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22 pages, 5496 KB  
Article
Design Optimization of RF MEMS-Driven Triangular Resonators with Sierpinski Geometry for Dual-Band Applications
by Alina Cismaru, Flavio Giacomozzi, Mircea Pasteanu and Romolo Marcelli
Micromachines 2025, 16(4), 446; https://doi.org/10.3390/mi16040446 - 9 Apr 2025
Cited by 1 | Viewed by 2306
Abstract
This paper proposes a detailed design study of resonating high-frequency notch filters driven by RF MEMS switches and their optimization for dual-band operation in the X-Band. Microstrip configurations will be considered for single and dual-band applications. An SPDT (single-pole-double-thru) switch composed of double-clamped [...] Read more.
This paper proposes a detailed design study of resonating high-frequency notch filters driven by RF MEMS switches and their optimization for dual-band operation in the X-Band. Microstrip configurations will be considered for single and dual-band applications. An SPDT (single-pole-double-thru) switch composed of double-clamped ohmic microswitches has been introduced to connect triangular resonators with Sierpinski geometry, symmetrically placed with respect to a microstrip line to obtain a dual notch response. Close frequencies or spans as wide as 2 GHz can be obtained depending on the internal complexity and the edge side. The internal complexity has been modified to introduce the possibility of using the same edge size for the frequency tuning of an elementary cell, maintaining a fixed footprint, and allowing coupled structures to implement high-frequency filters of the same size and variable operational frequencies. Preliminary experimental results have been obtained as a confirmation of the predicted device functionality. Full article
(This article belongs to the Special Issue The 15th Anniversary of Micromachines)
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24 pages, 4831 KB  
Article
Analysis and Evaluation of the Experimental Effect of Double-Disc Knife-Cutting Device for Carrot Combine Harvester
by Bokai Wang, Zhichao Hu, Fengwei Gu, Feng Wu, Mingzhu Cao and Yunjing Sun
Agriculture 2025, 15(7), 682; https://doi.org/10.3390/agriculture15070682 - 24 Mar 2025
Cited by 1 | Viewed by 792
Abstract
At present, the problems of the low cutting reliability and poor cutting quality of carrot harvesters in China are particularly prominent, directly leading to the problems of high root and stem damage rates, low stem and leaf cutting rates, and low cutting surface [...] Read more.
At present, the problems of the low cutting reliability and poor cutting quality of carrot harvesters in China are particularly prominent, directly leading to the problems of high root and stem damage rates, low stem and leaf cutting rates, and low cutting surface flatness rates. In order to solve these problems, we developed a disc-type double-disc cutting device. Based on the structural analysis and the central combination design theory of Box–Behnken, using three factors as influencing factors, namely, clamping and conveying speed, the rotary speed of the disc cutter, and the thickness of the disc cutter. A response surface experiment was carried out to analyze the influence of each factor on the high damage rates of the rhizome, the clean rates of stems and leaves, and the flatness rate of cutting surfaces to optimize the influencing factors. According to the test results, a regression mathematical model between test parameters and performance indexes was established, and optimization verification was carried out according to the regression model between test factors and indexes. Finally, the optimal parameter combination is as follows: a clamping and conveying speed of 1.0 m/s, a rotary speed of the disc cutter of 193.5 r/min, and a thickness of the disc cutter of 3.6 mm. The results of the field experiment showed that the root and stem damage rate was 2.61%, the stem and leaf-cutting rate was 87.32%, and the cutting surface flatness rate was 89.87%. Compared with a set of parameters commonly used in double-disc cutters to harvest carrots under the same conditions, the corresponding root and stem damage rates, stem and leaf-cutting rates, and productivity decreased by 2.16%, 1.97%, and 1.87%, respectively, and the comprehensive performance was obviously improved. The proposed research method can well simulate the cutting process in carrot harvesting and provide support for the development of carrot harvesting equipment. Full article
(This article belongs to the Section Agricultural Technology)
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21 pages, 7487 KB  
Article
Free- and Forced-Vibration Characteristic Analysis of a Double-Layered Cylindrical Shell with General Boundary Conditions
by Jianghai Wu, Hongzhen Zhu and Yong Duan
J. Mar. Sci. Eng. 2025, 13(4), 641; https://doi.org/10.3390/jmse13040641 - 24 Mar 2025
Cited by 1 | Viewed by 659
Abstract
The double-layered cylindrical shell represents a key structural configuration for underwater vehicles, where its vibration behavior remains a primary concern in engineering design and analysis. This study develops a spectral element method (SEM) for dynamic modeling of multi-component shell systems by extending the [...] Read more.
The double-layered cylindrical shell represents a key structural configuration for underwater vehicles, where its vibration behavior remains a primary concern in engineering design and analysis. This study develops a spectral element method (SEM) for dynamic modeling of multi-component shell systems by extending the vibrational governing equations of conical shells. The methodology is validated through finite element method (FEM) case studies on both conical shells and double-layered cylindrical configurations. Parametric investigations examine ribbed substructures and solid rib plates within the cylindrical shell assembly, while artificial spring techniques model arbitrary boundary conditions—with validation against classical benchmarks confirming their effectiveness for elastic constraints. Numerical demonstrations reveal the following: rib and plate thickness variations exhibit a negligible impact on low-frequency vibrational responses; the natural frequency sensitivity peaks when the elastic boundary stiffness approaches the inherent dynamic stiffness of the shell’s base configuration, while extreme stiffness values approximate clamped or free boundary conditions with engineering significance. The proposed SEM framework demonstrates a superior computational efficiency and accuracy compared to conventional FEM approaches. These findings deliver practical guidance for marine structural engineering, particularly in the boundary condition specifications and performance optimization of composite shell systems. Full article
(This article belongs to the Section Ocean Engineering)
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16 pages, 5201 KB  
Article
Robotic Fast Patch Clamp in Brain Slices Based on Stepwise Micropipette Navigation and Gigaseal Formation Control
by Jinyu Qiu, Qili Zhao, Ruimin Li, Yuzhu Liu, Biting Ma and Xin Zhao
Sensors 2025, 25(4), 1128; https://doi.org/10.3390/s25041128 - 13 Feb 2025
Viewed by 1102
Abstract
The patch clamp technique has become the gold standard for neuron electrophysiology research in brain science. Brain slices have been widely utilized as the targets of the patch clamp technique due to their higher optical transparency compared to a live brain and their [...] Read more.
The patch clamp technique has become the gold standard for neuron electrophysiology research in brain science. Brain slices have been widely utilized as the targets of the patch clamp technique due to their higher optical transparency compared to a live brain and their intercellular connectivity in comparison to cultured single neurons. However, the narrow working space, small scope, and depth of the field of view make the positioning of the operation’s micropipette to the target neuron a time-consuming task reliant on a high level of experience, significantly slowing down operation of the patch clamp technique in brain slices. Further, the current poor controllability in gigaseal formation, which is the key to electrophysiology signal recording, significantly lowers the patch clamp success rate. In this paper, a stepwise navigation of the micropipette is conducted to accelerate the positioning process of the micropipette tip to the target neuron in the brain slice. Then, a fuzzy proportional–integral–derivative controller is designed to control the gigaseal formation process along a designed resistance curve. The experimental results demonstrate an almost doubled patch clamp technique speed, with a 25% improvement in the success rate compared to the conventional manual method. The above advantages may promote the application of our method in brain science research based on brain slice platforms. Full article
(This article belongs to the Special Issue Advanced Sensors and AI Integration for Human–Robot Teaming)
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15 pages, 3846 KB  
Article
Model of Quality Factor for (111) 3C-SiC Double-Clamped Beams
by Angela Garofalo, Annamaria Muoio, Sergio Sapienza, Matteo Ferri, Luca Belsito, Alberto Roncaglia and Francesco La Via
Micromachines 2025, 16(2), 148; https://doi.org/10.3390/mi16020148 - 28 Jan 2025
Cited by 1 | Viewed by 2838
Abstract
Silicon carbide (SiC) is an interesting semiconductor for MEMS devices. The high-value Young’s modulus of silicon carbide facilitates high frequencies and quality (Q) factors in resonant devices built with double-clamped beams. The aim of this work is to achieve the determination and modeling [...] Read more.
Silicon carbide (SiC) is an interesting semiconductor for MEMS devices. The high-value Young’s modulus of silicon carbide facilitates high frequencies and quality (Q) factors in resonant devices built with double-clamped beams. The aim of this work is to achieve the determination and modeling of the Q-Factor for samples of micromachined 3C-SiC film on <111> silicon substrates. This study demonstrates that the experimental datasets created by Romero, integrated with the thicker samples reported in this work, fit the theoretical model presented in the paper. Furthermore, the influence of the crystallographic defects present at the 3C-SiC/Si interface on the Q-factor can be observed both in the analytical model of Romero and in the numerical model present in COMSOL. 3C-SiC layers with thickness greater than 600 nm are needed to achieve an ideal performance from double-clamped beams. Full article
(This article belongs to the Special Issue SiC Based Miniaturized Devices, 3rd Edition)
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18 pages, 3589 KB  
Article
Addressing the Evolution of Cardenolide Formation in Iridoid-Synthesizing Plants: Site-Directed Mutagenesis of PRISEs (Progesterone-5β-Reductase/Iridoid Synthase-like Enzymes) of Plantago Species
by Maja Dorfner, Jan Klein, Katharina Senkleiter, Harald Lanig, Wolfgang Kreis and Jennifer Munkert
Molecules 2024, 29(23), 5788; https://doi.org/10.3390/molecules29235788 - 7 Dec 2024
Cited by 1 | Viewed by 1207
Abstract
Enzymes capable of processing a variety of compounds enable plants to adapt to diverse environmental conditions. PRISEs (progesterone-5β-reductase/iridoid synthase-like enzymes), examples of such substrate-promiscuous enzymes, are involved in iridoid and cardenolide pathways and demonstrate notable substrate promiscuity by reducing the activated C=C double [...] Read more.
Enzymes capable of processing a variety of compounds enable plants to adapt to diverse environmental conditions. PRISEs (progesterone-5β-reductase/iridoid synthase-like enzymes), examples of such substrate-promiscuous enzymes, are involved in iridoid and cardenolide pathways and demonstrate notable substrate promiscuity by reducing the activated C=C double bonds of plant-borne and exogenous 1,4-enones. In this study, we identified PRISE genes in Plantago media (PmdP5βR1) and Plantago lanceolata (PlP5βR1), and the corresponding enzymes were determined to share a sequence identity of 95%. Despite the high sequence identity, recombinant expressed PmdP5βR1 was 70 times more efficient than PlP5βR1 for converting progesterone. In order to investigate the underlying reasons for this significant discrepancy, we focused on specific residues located near the substrate-binding pocket and adjacent to the conserved phenylalanine “clamp”. This clamp describes two phenylalanines influencing substrate preferences by facilitating the binding of smaller substrates, such as 2-cyclohexen-1-one, while hindering larger ones, such as progesterone. Using structural analysis based on templates PDB ID: 5MLH and 6GSD from PRISE of Plantago major, along with in silico docking, we identified positions 156 and 346 as hot spots. In PlP5βR1 amino acid residues, A156 and F346 seem to be responsible for the diminished ability to reduce progesterone. Moreover, the double mutant PlP5βR_F156L_A346L, which contains the corresponding amino acids from PmdP5βR1, showed a 15-fold increase in progesterone 5β-reduction. Notably, this modification did not significantly alter the enzyme’s ability to convert other substrates, such as 8-oxogeranial, 2-cyclohexen-1-one, and methyl vinyl ketone. Hence, a rational enzyme design by reducing the number of hotspots selectively, specifically improved the substrate preference of PlP5βR1 for progesterone. Full article
(This article belongs to the Special Issue Metabolites of Biofunctional Interest from Plant Sources)
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13 pages, 6406 KB  
Article
An Experimental Study on Vortex-Induced Vibration Suppression of a Long Flexible Catenary Cable by Using Vibration Dampers
by Li Ruan, Hongzhong Zhu and Changhong Hu
J. Mar. Sci. Eng. 2024, 12(11), 1995; https://doi.org/10.3390/jmse12111995 - 6 Nov 2024
Viewed by 1292
Abstract
In this paper, an experimental study is conducted to investigate the effectiveness of vibration dampers in suppressing vortex-induced vibration in a long, flexible catenary cable with a low mass ratio. The dampers, consisting of two small, symmetric, lightweight pipes clamped to the cable, [...] Read more.
In this paper, an experimental study is conducted to investigate the effectiveness of vibration dampers in suppressing vortex-induced vibration in a long, flexible catenary cable with a low mass ratio. The dampers, consisting of two small, symmetric, lightweight pipes clamped to the cable, are sparsely deployed along the cable to shape the vibration characteristics. The experimental results demonstrate that dampers significantly reduce the vibration amplitude by up to 60% and axial tension by up to 61% at high flow velocities, effectively suppressing the cable vibration in perpendicular flow. In addition, it is observed that the in-line and cross-flow vibration frequencies are approximately equal when the dampers are applied. This behavior contrasts with the conventional undamped catenary cable, where the in-line vibration frequencies are double those of the cross-flow frequencies. Full article
(This article belongs to the Special Issue Safety and Reliability of Ship and Ocean Engineering Structures)
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13 pages, 9651 KB  
Article
Design and Fatigue Life Analysis of the Rope-Clamping Drive Mechanism in a Knotter
by Jianjun Yin, Zefu Chen, Shiyu Lv, Han Wu, Yansu Gao and Luning Wu
Agriculture 2024, 14(8), 1254; https://doi.org/10.3390/agriculture14081254 - 30 Jul 2024
Cited by 2 | Viewed by 1592
Abstract
A knotter is a core component for the automatic bundling of agricultural materials, and a knotter with double-fluted discs is one type. Currently, the research on knotters with double-fluted discs has gradually transitioned from structural design to reliability optimization. To address rope-clamping failures [...] Read more.
A knotter is a core component for the automatic bundling of agricultural materials, and a knotter with double-fluted discs is one type. Currently, the research on knotters with double-fluted discs has gradually transitioned from structural design to reliability optimization. To address rope-clamping failures in the rope-clamping drive mechanisms in knotters, the specific failure position of the rope-clamping mechanism and the failure causes were analyzed first. The redesign of the rope-clamping drive mechanism in knotters with double synclastic fluted discs was proposed, including structure optimization and 3D modeling using the GearTrax/KISSsoft and SolidWorks software. A virtual prototype model of a knotter with a flexible rope was established by combining ANSYS with the ADAMS software. A rigid–flexible coupling dynamic simulation of the knotter was carried out using ADAMS, and the simulation results were used as the data input for the ANSYS nCode DesignLife module for the fatigue life simulation of the weak parts (the worm shaft) of the knotter. The operation test results for the rope-clamping drive mechanism indicate that the redesigned rope-clamping drive mechanism is reliable in transmission, with a rope-clamping success rate of 100%. The actual operation times for the worm shaft exceed the minimum fatigue life obtained through joint simulation. The applied joint simulation method has high simulation accuracy. Full article
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