Search Results (2,359)

Japanese quail production can be optimized by selecting appropriate dietary lipid sources, yet comparative effects on performance and egg quality during the laying phase are not fully established. This study evaluated the impact of five lipid sources, namely soybean oil, corn oil, canola oil, sunflower oil, and poultry fat, on performance, egg quality, nutrient metabolism, serum metabolites, and organ traits of 350 Japanese quail aged 60 days with an average weight of 170 ± 10 g. Birds were assigned to diets containing 2800 kcal/kg in a completely randomized design with 10 replicates of seven birds each. Performance was recorded over three 28-day periods and egg quality assessed at the end of each period; at 84 days, one bird per replicate was sampled for nutrient metabolism, serum metabolites, and organ characteristics, and a metabolism trial estimated metabolizability coefficients and metabolizable energy. Data were analyzed by Tukey’s test at the 5% level. Egg production (p = 0.010) and marketable egg production (p = 0.008) were highest with soybean, corn, and sunflower oils, while feed conversion per dozen eggs was less efficient with canola oil (p = 0.048). Egg quality differed in specific gravity (p = 0.027), yolk color (p = 0.008), Haugh unit (p = 0.011), and air cell size (p = 0.001), with poultry fat improving yolk color and Haugh unit. Canola oil increased dry matter (p = 0.027) and ether extract digestibility (p = 0.026), while serum metabolites, organ weights, and reproductive traits were not affected (p > 0.05). All diets supported physiological health, and lipid sources can be chosen according to cost and availability to optimize quail production without compromising performance or health. Full article

This study presents the design and simulation of an integrated pathway to produce Biodimethyl ether (Bio-DME) from biomethane derived from Agave sisalana residues, focusing on the downstream sections such as: (i) steam reforming of biogas and water-gas shift to generate syngas and (ii) indirect methanol synthesis followed by methanol dehydration to Bio-DME, including separation and recycle steps. The modeled scope excludes the anaerobic digestion stage. Benchmarking against the literature was used to validate model fidelity. The simulation delivered a single-pass methanol conversion of 81.8%, a Bio-DME reactor conversion of 44.6 mol%, and a Bio-DME yield/selectivity of ≈99 mol%; product purities reached ≈99.99 mol% Bio-DME at the first distillation column and ≈99.9 mol% MeOH in the recycle, indicating efficient separation. Compared to the literature, Bio-DME conversion in this study is slightly below the reported values (0.446 vs. 0.499, Δ = 0.053), while yield is very close to literature (0.99 vs. 0.9979, Δ = 0.0079). Incomplete methanol conversion emerges as the primary optimization lever, pointing to adjustments in operating conditions (T, p), recycle/purge strategy, and H₂/CO control. Overall, the results confirm the technical feasibility of the simulated sections and support the development of a sisal-based, low-carbon Bio-DME route relevant to Northeast Brazil. Full article

As the environmental burden of traditional plastics continues to grow, bioplastics (BPs) have emerged as a promising alternative due to their renewable origins and potential for biodegradability. However, the most popular anaerobic systems (ASs)—anaerobic digestion (AD), acidogenic fermentation (AF), and enzyme hydrolysis (EH)—for BPs degradation still face many challenges, e.g., low degradation efficiency, process instability, etc. As a sustainable clean energy technology, bioelectrochemical systems (BESs) have demonstrated strong potential in the treatment of complex organic waste when integrated with ASs. Nevertheless, research on the synergistic degradation of BPs using BES-ASs remains relatively limited. This review systematically summarizes commonly used anaerobic degradation methods for BPs, along with their advantages and limitations, and highlights the BES-AS as an innovative strategy to enhance BPs degradation efficiency. BESs can accelerate the decomposition of complex polymer structures through the activity of electroactive microorganisms, while also offering benefits such as energy recovery and real-time process monitoring. When coupled with anaerobic digestion, the BES-AS demonstrates significant synergistic effects, improving degradation efficiency and promoting the production of high-value-added products such as volatile fatty acids (VFAs) and biogas, thereby showing great application potential. This review outlines current research progress, identifies key knowledge gaps in mechanism elucidation, system design, source recovery, etc., and proposes future research directions. These include system optimization, microbial community engineering, development of advanced electrode materials, and omics-based mechanistic studies. Advancing multidisciplinary integration is expected to accelerate the practical application of BES-ASs in BP waste management and contribute to achieving the goals of sustainability, efficiency, and circular utilization. Full article

This study evaluated how dietary supplementation with Haematococcus pluvialis powder (HPP), Arthrobacter bussei powder (ABP), and A. bussei probiotics affects growth, whole-body composition, non-specific immunity, antioxidant capacity, and nutrient digestibility in Pacific white shrimp (Litopenaeus vannamei). Juvenile shrimps were fed for 8 weeks with five diets: a control diet (CON), H. pluvialis powder (HPP, 1%), A. bussei powder (ABP, 1%), or A. bussei probiotics at 10⁵ (ABL) or 10⁸ (ABH) CFU g⁻¹ feed. Shrimp fed the ABP diet exhibited the highest final body weight, weight gain, and protein efficiency ratio, with a significantly improved feed conversion ratio than that of CON, ABL, and ABH groups. The HPP group exhibited significantly better growth than that of the control. Regarding immunity and antioxidant responses, lysozyme and phenoloxidase specific activities, as well as glutathione peroxidase and superoxide dismutase specific activities, were significantly enhanced in shrimp fed ABP and HPP diets, whereas malondialdehyde levels were significantly reduced compared with those in CON. Apparent crude protein digestibility was significantly higher in all supplemented diets than those in the CON group, with ABP showing the highest value. ABP and HPP supplementation improved growth, protein digestibility, and immune-antioxidant responses in L. vannamei, whereas probiotic forms showed limited effects. ABP is a superior functional feed additive than its probiotic form for enhancing productivity and health in shrimp aquaculture. Full article

This study presents a novel approach to intensifying the anaerobic digestion of sewage sludge through enzymatic pretreatment using hydrolytic enzymes—cellulase and lysozyme. It aims to determine how enzymatic activation affects the efficiency of methane fermentation, defined as the degree of organic matter decomposition and yield and composition of biogas. An experiment was carried out under mesophilic conditions over 20 days, analyzing the physicochemical properties of sludge, biogas production, methane content, and sanitary parameters. The addition of cellulase and lysozyme significantly enhanced process efficiency, increasing both the rate of organic matter degradation and biogas yield. The highest biogas production values (0.73 L·g⁻¹ d.m. for cellulase and 0.72 L·g⁻¹ d.m. for lysozyme) were obtained at a 4% (w/w) enzyme concentration, with a corresponding increase in the degree of organic matter decomposition to 78.7% and 80.0%, respectively. The produced biogas contained 58–61% methane, exceeding the values observed in the control sample, which indicates a positive effect of enzymatic activation on methane selectivity. Enhanced biogas production was attributed to improved hydrolysis of complex organic compounds, resulting in greater substrate bioavailability for methanogenic microorganisms. Moreover, methane fermentation led to the complete elimination of E. coli from all supernatants, confirming the hygienization potential of the process. The results of this study indicate that enzymatic pretreatment may serve as a viable strategy to improve both the energy efficiency and hygienic safety of anaerobic digestion processes, with relevance for future optimization and full-scale wastewater treatment applications. Full article

The growing consumption of oilseed-pressed cakes (OSCs), a largely underutilized feedstock, plays a significant role in animal feed. The study evaluates the use of three OSCs—flax (FSC), pumpkin (PSC), and hemp (HSC)—as substrates for Bacillus subtilis ATCC 6051a (BS) in a solid-state fermentation (SSF) to enhance enzyme production and probiotic viability. The SSF process was assessed to evaluate the microbial growth, sporulation efficiency, enzymatic activity (protease, cellulase, xylanase, and phytase), and in vitro digestibility of fermented substrates. The results indicate that bacterial growth and sporulation varied significantly among substrates (p < 0.05). FSC presents the highest spore resistance (86.52%), followed by PSC (82.87%) and HSC (81.29%). Notably, protease was highest in HSC (184.67 U/g), while FSC supported maximum cellulase activity. HSC exhibited superior xylanase (1.86 ± 0.043 U/g DW, p < 0.05) and phytase production, while pH analysis indicated a shift toward alkalinity in PSC and HSC due to proteolytic activity. FSC maintained the most stable bacterial population during digestion, suggesting its potential as a probiotic carrier. These findings highlight that fermentation of OSCs with BS improved their nutritional value and can be used as a sustainable solution in feeding programs for piglets. Full article

Precision Feeding Systems (PFS) demonstrate transformative potential in advancing sustainable and efficient production within modern animal husbandry. However, existing research lacks a synthesis of PFS applications in livestock farming and offers little targeted guidance for China’s rapidly growing rabbit industry. The objective of this review is to bridge this gap by synthesizing current knowledge on PFS technologies—including sensor networks, artificial intelligence (AI), automated controls, and data analytics—and providing a structured framework for their implementation in rabbit production. This study selects and analyzes 112 core references, establishing a foundational database for comprehensive evaluation. The key contributions of this work are threefold: first, it outlines the core components and operational mechanisms of PFS; second, it identifies major challenges such as sensor reliability in dynamic environments, data security risks, limited explainability of AI models, and interoperability barriers; and third, it proposes a customized strategy for PFS adoption in rabbit farming, emphasizing phased implementation, cross-system integration, and iterative optimization. The primary outcomes and advantages of adopting such a system include significant improvements in feed efficiency, resource utilization, animal welfare, and waste reduction—critical factors given rabbits’ sensitive digestive systems and precise nutritional needs. Furthermore, this review outlines a future research agenda aimed at developing resilient sensors, explainable AI frameworks, and multi-objective optimization engines to enhance the commercial scalability and sustainability of PFS in rabbit husbandry and beyond. Full article

Feed and water scarcity are major challenges for the sustainability of livestock production, particularly in semi-arid regions with structural limitations in resource availability. In this context, the valorization of agro-industrial by-products contributes to circular agriculture, reduces waste, and promotes more efficient resource use, in line with the United Nations Sustainable Development Goals. This study evaluated the inclusion of partial mixed tomato residue (PMR) silage in sheep diets and its effects on productive performance, total water intake, and meat quality. Eighteen ewe lambs were assigned to two groups: control (concentrate and deferred pasture) and PMR (tomato residue silage and deferred pasture). The PMR silage had a pH of 3.97 and was mainly characterized by lactic and acetic acids, with minor amounts of propionic and butyric acids. The butyric acid concentration (8.9 g kg⁻¹ DM) slightly exceeded the recommended threshold (0.5% DM), suggesting some clostridial activity but remaining below levels associated with severe deterioration. Animals fed PMR silage showed a 36% higher dry matter intake (p = 0.001), with greater intake of total digestible nutrients and fiber. This translated into a 54% higher average daily gain (p = 0.02) and an 11% greater final body weight compared with the control group (p = 0.02). Dietary water intake was also higher in the PMR group, reducing direct water consumption from drinkers by 38% (p < 0.001). Meat quality parameters were unaffected by the diet. Pesticide residue screening by LC-MS/MS revealed no detectable levels of abamectin, cymoxanil, chlorothalonil, difenoconazole, or mancozeb in silage. In meat samples, only chlorothalonil was tested and it was not detected. However, the use of PMR silage increased direct energy demand due to transport and compaction, while feeding costs per unit of weight gain were reduced. Overall, PMR silage proved to be a safe, fermentatively stable, and effective feeding alternative that enhances performance, reduces direct water intake, and maintains meat quality, representing a viable strategy for small ruminant production in water-limited regions. Full article

In this study, the electrospinning technique was employed to encapsulate mountain germander (MG) polyphenolic extract into pullulan/zein (PUL:ZE) delivery systems stabilized with sunflower lecithin. The rheological and physical properties of the pullulan (PUL), PUL:ZE, and zein (ZE) polymer solutions were evaluated to assess their electrospinnability potential. Fabricated nanofibers were then characterized for their morphology, physicochemical, and thermal properties, as well as encapsulation efficiency and simulated in vitro digestion. The elastic component of the polymer solution, quantified by the Deborah number, showed a strong correlation with nanofiber diameter (r = 0.75). FT-IR spectra confirmed the role of sunflower lecithin as a mediator in the formation of hydrogen and hydrophobic interactions among PUL, ZE, and polyphenols. The circular dichroism spectra confirmed the influence of the MG extract on the change in the secondary conformation of the protein structure. The PUL:ZE delivery matrix proved to be suitable for the retention of phenylethanoid glycosides (encapsulation efficiency > 73%). The formulation 50PUL:50ZE was found to have the highest potential for prolonged release of polyphenols under gastrointestinal in vitro conditions. These findings propose a water-based electrospinning approach for designing polyphenolic delivery systems stabilized with lecithin for potential applications in active food packaging or nutraceutical products. Full article

The aim of this study was to determine the effects of low-temperature pretreatment of microalgae (Tetraselmis subcordiformis (Wille) Butcher) and cyanobacteria (Limnospira platensis (Gomont) Ciferri et Tiboni) using solidified carbon dioxide (SCO₂) on the progression of methane fermentation. The experiment was carried out under batch conditions with six process variants that differed in the volumetric ratio of SCO₂ to the biomass tested. Changes in organic matter solubility, anaerobic digestion kinetics and overall CH₄ production performance were analysed. The results showed that pretreatment effectively increased the solubility of organic compounds, especially in the case of L. platensis biomass, where the highest increases in soluble sTOC (up to 21.6%) and sCOD (up to 14.3%) were observed. CH₄ yield in the most efficient variant (SCO₂:biomass = 1:2.5) increased to 354 ± 16 mL CH₄/gVS for T. subcordiformis and 403 ± 18 mL CH₄/gVS for L. platensis, respectively. Despite the apparently less favourable physicochemical parameters of the biomass for anaerobic digestion, L. platensis showed a higher susceptibility to digestion and better kinetic indicators for methane fermentation. The results indicate that the efficiency of anaerobic biodegradation of biomass depends not only on the chemical composition but also on the cellular structure and physicochemical interactions during pretreatment. The use of SCO₂ as a disintegrant could be an effective, energy-saving method to increase the fermentation efficiency of photosynthetic microorganisms in biowaste management. Full article

Olive cultivation is one of the most widespread agro-industrial activities in the Mediterranean area. However, required pretreatments often affect the anaerobic digestion process, promoting or inhibiting the overall yield. Therefore, the efficiency of Anaerobic Digestion (AD) processes cannot be established in advance but needs to be experimentally validated for each biomass-pretreatment combination. Following the present purpose, these biomasses were firstly treated: the olive pomace (OP) with a procedure based on the use of an ionic liquid (IL) composed of triethylamine and sulfuric acid [Et₃N][HSO₄] to remove hemicellulose and lignin and recover the insolubilized OP, while olive mill wastewater (OW) was processed via freeze-drying. The resulting materials, the pulp from olive pomace (POP) and freeze-dried OW (FDOW), were then digested using lab-scale anaerobic reactors. The biogas production was then compared with the quantity obtained by digesting the same untreated biomasses (OW and OP). The FDOW showed the highest biogas production due to the freeze-drying treatment that led to some morphological and structural surface modifications of OW (respectively, 658 mL vs. 79 mL/g for the two matrices), prompting microorganism activity. Conversely, the method based on the use of IL significantly reduced the nitrogen content of POP, thus resulting in the lowest biogas production, which ceased by the second day. To address this issue, we co-digested POP with the brewery’s spent grain, a biomass rich in nitrogen. This step enhanced the biogas yield of POP, resulting in an extended anaerobic digestion period and the production of 466 mL/g. Additionally, we tested FDOW in co-digestion with BSG to evaluate improvements in production. The codigestion of the two matrices increased the biogas yield of FDOW from 944 to 1131 mL/g. Full article

Background: The water-soluble Grifola frondosa polysaccharides (GFPs) are the primary bioactive component of the edible and medicinal fungus Grifola frondosa. However, the digestive behavior of GFPs in the human gastrointestinal (GI) tract and their subsequent interaction with gut microbiota (GM) to exert health effects remain unclear. Methods: In this study, GFPs were extracted based on a traditional hot water decoction. An in vitro simulated GI digestion model and a human fecal microbiota fermentation model were established to systematically investigate the digestive stability of GFPs, GM modulation, and metabolite changes. Results: Results showed that GFPs remained structurally stable during in vitro oral, gastric, and small intestinal digestion, allowing them to reach the colon intact for microbial fermentation. During colonic fermentation, GFPs were efficiently degraded by GM, and significantly increased the relative abundance of beneficial bacteria such as Akkermansia, Bacteroides, Parabacteroides, and Lactobacillus while reducing the abundance of pathogenic Escherichia-Shigella. Meanwhile, GFPs enriched metabolites beneficial for intestinal health, among which γ-aminobutyric acid (GABA) was the most significantly upregulated. Single-strain fermentation confirmed that Lactobacillus (L. plantarum) was the core GABA-producing genus. Conclusions: This study highlights the potential of GFPs as prebiotics for GM modulation, expands the understanding of the health-promoting effects of fungal polysaccharides, and provides a theoretical basis for the development of GFP-based functional foods. Full article

The cherry barb (Puntius titteya) is a staple of the ornamental aquaculture industry, with an estimated 60,000 individuals produced monthly in Florida, USA. On a commercial scale, small improvements in efficiency may yield considerable economic and operational benefits. This study investigated first-feeding protocols aimed at reducing the use of live Artemia spp. nauplii by evaluating microdiets (MDs) top-dressed with feed attractants and commercial liquid Artemia replacements (LAs). Larvae were fed MDs top-dressed with 0.25%, 0.50%, or 1.0% of L-alanine, betaine, or L-tryptophan for seven days. Diets with L-alanine and L-tryptophan significantly increased survival compared to the reference diet. A 21-day trial that tested three feed attractants combined into a single diet at previously determined inclusion levels (L-alanine, 0.5%; betaine, 0.25%; and L-tryptophan, 0.25%) showed no additive or synergistic survival benefits. No differences were observed for RNA/DNA ratios at 15 days post-hatch, suggesting no effect on larval quality. Additional experiments were conducted comparing the performance of two commercial LA diets (EZ Artemia Ultra [Zeigler Bros., Inc., Gardners, PA, USA] and LiquaLife^® [Cargill Inc., Minneapolis, MN, USA]) against live Artemia. Partial Artemia replacement with EZ Artemia Ultra maintained comparable survival to larvae fed only Artemia. Larval growth was significantly reduced in 100% LA groups, suggesting limitations in nutrition or digestibility. These findings demonstrate that targeted use of feed attractants can enhance early rearing outcomes in P. titteya aquaculture, facilitating the goal of reducing Artemia use and increasing production efficiency and hatchery output. Full article

To enhance the high-value utilization of camellia oil and innovation in functional foods, this study developed a stable emulsion template using xanthan gum (XG) and sodium caseinate (CAS) for the preparation of camellia oil microcapsules via spray drying. Employing scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), and thermogravimetric analysis (TGA), alongside additional analytical methods, this study systematically examined the influence of drying temperature (145 °C, 165 °C, and 185 °C) and XG concentration (0.2%, 0.3%, and 0.4%) on the physicochemical properties and functional attributes of the microcapsules. Results indicated that 0.3% XG was the optimal concentration, enabling uniform emulsion droplet dispersion while balancing microcapsule bulk density and solubility, thereby optimizing processing and dissolution properties. 165 °C was identified as the optimal drying temperature, yielding the highest microcapsule yield (53.68%), moisture content (<2.84%) meeting storage standards, and optimal β-carotene encapsulation efficiency (89.6%) and DPPH radical scavenging rate (74.80 ± 0.34%). FTIR analysis confirmed successful encapsulation of camellia oil within microcapsules. TGA and in vitro digestion experiments demonstrated excellent thermal stability and digestive characteristics of the microcapsules. In summary, this study identified the most favorable preparation conditions for camellia oil microcapsules, providing theoretical support and technical reference for expanding camellia oil applications in the food industry. Full article

This work focuses on assessing the potential for energy recovery from biomass obtained from unproductive lands in the Jiu Valley. The main objective of the study is to identify a viable solution for the restoration of degraded land. The research aims to utilise these lands through energy crops; the biomass is then introduced into an anaerobic digestion plant to produce biogas. The novelty of this research consists of the physical transformation of these lands into a living laboratory, intended exclusively for the cultivation and study of biomass. Maize, soybean and sorghum plants were chosen for the study. The biomass was analysed in detail to determine the total solids (TS) and volatile solids (VS) content. In maize, VS ranged from 62.67% by mass to 76.58% by mass. In soybean, VS content was between 35.35% by mass and 36.60% by mass. In sorghum, VS ranged between 35.29% by mass and 38.86% by mass. This research showed that the largest amount of biogas resulted from fresh soybean biomass. Dry biomass, with its high lignin content, significantly reduced the digestion process’s efficiency. The study highlights that it is important to use fresh biomass after an appropriate mechanical pre-treatment to optimise biogas production. Full article