Search Results (75,102)

Studies show microplastics (MPs) impair lung function directly and indirectly, yet effective solutions are lacking. In light of this, sesamin (Ses), a natural lignan-like compound with diverse pharmacological properties, may offer protection. The study aims to investigate whether Ses pretreatment can mitigate MPs-induced lung damage and to elucidate the underlying mechanisms. Male C57BL/6 mice received MPs (10,000 μg/L) in drinking water, with varying Ses doses gavaged daily for 28 days. Computational pharmacology and in vivo/in vitro experiments, including histology, immunofluorescence, and western blot, were used to elucidate Ses’s protective mechanisms. In vivo experiments showed Ses can alleviate MPs-induced histopathological alterations, inflammatory responses, and oxidative stress in lung tissue. Computational pharmacology suggested that the protective mechanism of Ses may be associated with the apoptotic signaling pathway, with Bcl2 as its potential target. Both in vivo and in vitro studies demonstrated that Ses significantly upregulates Bcl2 expression while downregulating Bax and Casp3. Notably, a Bcl2 inhibitor substantially attenuated Ses’s protective effects. Our research suggests that Ses can mitigate MPs-induced lung injury by modulating the apoptotic signaling pathway, with Bcl2 identified as a key target. Dietary supplementation may represent a promising intervention strategy for preventing and managing food safety risks associated with MPs. Full article

To investigate the effect of different grouting pressures on the reinforcement of fractured sandy mudstone, grouting tests, mechanical experiments, CT scanning, and SEM analysis were conducted on fractured rock samples. Based on CT data, the precise internal structure of the grouted rock samples was obtained. High-fidelity numerical models were constructed in ABAQUS through image processing and mesh mapping techniques and then imported into ANSYS for uniaxial compression simulation. The results showed that under grouting pressures of 1 MPa, 3 MPa, and 5 MPa, the compressive strengths of the samples were 10.08 MPa, 12.39 MPa, and 13.28 MPa, corresponding to increases of 22.9% and 7.2%, respectively. The elastic moduli were 1.16 GPa, 1.52 GPa, and 1.63 GPa, with increases of 31% and 7.2%, respectively. The toughness index and brittleness index exhibited opposite trends: the toughness index increased from 1.6555 to 1.7135 and then to 1.7648 (rises of 3.5% and 2.9%), while the brittleness index decreased from 1.5255 to 1.4020 and then to 1.3075 (reductions of 8.1% and 6.7%). The ductility index rose from 1.8760 to 2.0972 and then to 2.2637 (increases of 11.8% and 7.9%). The failure mode of the grouted rock samples shifted from brittle to ductile behavior, with the most pronounced overall mechanical improvement observed at 3 MPa grouting pressure. SEM analysis indicated that as the grouting pressure increased, the dominant crack type changed from large cracks to micro-cracks. At 3 MPa, the grout fully penetrated micro-pores and enhanced the sample’s integrity, whereas at 5 MPa, excessive grouting pressure induced damage to the rock matrix itself. Fracture simulations further demonstrated that as the grouting pressure increased from 1 MPa to 3 MPa and above, the failure mode shifted from being controlled by pre-existing fractures to a holistic rupture involving both the grout and the rock matrix, leading to significantly improved structural integrity. This study establishes an integrated numerical simulation approach of “CT scanning—in situ modeling—mechanical analysis”, providing a scientific basis for optimizing grouting parameters. Full article

Myocardial ischemia–reperfusion (I/R) injury remains a major contributor to infarct expansion and adverse cardiac remodeling despite advances in timely reperfusion therapy. Although restoration of blood flow is essential for myocardial salvage, the abrupt transition from ischemia to reperfusion paradoxically exacerbates cardiomyocyte injury through profound metabolic, ionic, and mitochondrial disturbances. Reperfusion should be viewed not simply as restoration of blood flow, but as a critical biological transition that converts ischemic stress into a self-amplifying injury network. Reperfusion induces excessive reactive oxygen species generation, calcium overload, endothelial barrier disruption, and dysregulated innate immune activation, which converge on mitochondrial dysfunction and diverse forms of cell death, including apoptosis, necroptosis, pyroptosis, and ferroptosis. Emerging evidence highlights that these pathological processes are tightly interconnected through damage-associated molecular pattern signaling, microvascular leakage, and inflammatory amplification, underscoring the limitations of single-target therapeutic approaches. This review summarizes the molecular and cellular mechanisms underlying myocardial I/R injury with a particular focus on oxidative stress, immune modulation, vascular integrity, and ferroptosis. We further discuss current and emerging cardioprotective strategies, including antioxidant therapies, modulation of neutrophil recruitment, microvascular leakage blockade, and anti-ferroptotic interventions. Finally, we address key translational challenges and future perspectives for developing integrated cardioprotective therapies aimed at improving clinical outcomes in acute myocardial infarction. Full article

The N88S mutation in human seipin causes a dominant motor neuron disease marked by ER stress and inclusion body formation, lipid imbalance, and oxidative damage. However, the metabolic mechanisms connecting these defects remain poorly understood. Previous proteomic profiling in our yeast model of N88S human seipinopathy revealed decreased protein levels of enzymes involved in the tricarboxylic acid cycle, fatty acid and carboxylic acid metabolism, and the glyoxylate cycle, suggesting impaired downstream utilization of peroxisome-derived acetyl-CoA. Guided by these findings, we investigated how peroxisomal function contributes to cellular dyshomeostasis. N88S seipin-expressing cells exhibited increased peroxisome abundance but defective routing of acetyl-CoA into mitochondrial and glyoxylate pathways, resulting in elevated reactive oxygen species (ROS), impaired glyoxylate cycle activation, and reduced metabolic adaptability to non-fermentable carbon sources. Loss of peroxisomes or forced cytosolic redirection of acetyl-CoA further exacerbated ER stress, ROS accumulation, lipid peroxidation, and the growth defect on N88S seipin-expressing cells, whereas inhibition of fatty acid synthesis mitigated oxidative damage. These findings demonstrate that N88S seipin triggers a futile cycle in which misrouted cytosolic acetyl-CoA drives lipogenesis, amplifying oxidative damage and ER stress. We conclude that defective peroxisome–mitochondria metabolic coupling and acetyl-CoA misrouting may represent central pathogenic mechanisms driving cellular dysfunction in N88S-linked seipinopathy. Full article

Malaria remains a major global health challenge, driven in part by widespread antimalarial drug resistance in Plasmodium parasites. Artemisinin-based combination therapies (ACTs) are currently the first-line treatment; however, resistance has also emerged. Artemisinin damages parasite proteins, promoting their ubiquitination and subsequent proteasomal degradation. Because inhibitors of the Plasmodium 26S proteasome synergize with artemisinin, the proteasome has emerged as a promising drug target, yet tools to monitor its function in live parasites remain limited. Here, we generated a P. falciparum line expressing green fluorescent protein fused to a destabilization domain (GFP-DD) to assess proteasome activity and combined it with MitoTracker^TM staining. In the absence of the stabilizing ligand Shield-1, the GFP-DD reporter is rapidly degraded by the proteasome. Using fluorescence microscopy and flow cytometry, we show that GFP-DD fluorescence provides a quantitative, inverse readout of proteasomal activity, increasing upon ligand-mediated stabilization or pharmacological inhibition with MG132. Shield-1 titration identified an optimal stabilization range, and MG132 induced a dose-dependent fluorescence increase. This work establishes a practical live-cell platform to probe ubiquitin–proteasome system function, with potential applications in future phenotypic screening and antimalarial resistance studies. Full article

The early warning of rock mass failure in deep hard-rock mines presents a significant challenge for mine safety management. Microseismic monitoring data offer a novel analytical approach to address this issue. This study investigates the evolutionary patterns of rock mass failure in mining areas through the analysis of spatiotemporal energy data from microseismic events. Initially, key spatiotemporal energy parameters are extracted to identify microseismic events associated with localized damage and their periodic characteristics. Subsequently, a spatiotemporal fractal dimension analysis method is established to achieve fractal interpretation of the data by integrating field cloud maps. Finally, an early warning model centered on temporal energy is constructed, which delineates warning zones through a comprehensive evaluation of fractal dimensions, thereby providing decision-making support for mine safety. Full article

With regard to space telescopes, the processing of large optical mirrors has always been a highlight in the field of optical processing. These mirrors are typically made of hard and brittle materials such as quartz glass, microcrystalline glass, and silicon carbide. These materials have long been considered challenging to work with due to their processing efficiency and propensity for damage. This study proposes a trochoid model considering the actual motion trajectory of the cup wheel with discrete consolidated abrasive grains. Through the establishment of a process parameter–mathematical model to establish the multi-grain coupled motion trajectory, the uniformity of the trajectory is optimized to increase the material removal rate and reduce the surface damage caused by abrasive interference. The results show that the process parameter optimization using this model can effectively reduce the surface roughness of quartz glass grinding. The surface and sub-surface damage caused by grinding stress are significantly reduced, and the edge fracture area of quartz glass is decreased. The large contact area at the end face of the cup-grinding wheel enables a larger grinding depth while ensuring that cracks do not extend to the sub-surface, improving the overall surface integrity of the mirror. Full article

High-strength concrete (HSC) is vital for large-scale tunnel infrastructure; however, its durability is often compromised by rigorous freeze–thaw cycles in cold-region environments. This study investigates the synergistic effects of incorporating hybrid steel fiber (SF) and polypropylene fiber (PPF) to enhance the frost resistance of HSC. Experimental testing involved 125 freeze–thaw cycles across various fiber dosages and lengths, monitoring mass loss and the relative dynamic modulus of elasticity. Additionally, a concrete damage plasticity (CDP) model was utilized in numerical simulations to analyze thermal stress distribution and damage evolution under coupled freeze–thaw and axial loading. Results indicate that the hybrid fiber integration significantly improved durability, with Group A3 (35 kg/m³ SF and 1.5 kg/m³ of 18 mm PPF) achieving the highest performance. After 125 cycles, Group A3 maintained a relative dynamic modulus of 94.5% and restricted mass loss to 1.42%, a 41% improvement over the fiber-free control. Numerical simulations corroborated these findings, demonstrating that the dual-fiber system preserves load-bearing capacity, limiting compressive strength degradation to just 6.7%. These findings quantitatively validate the synergistic mechanisms of hybrid fibers, providing a robust reference for designing high-durability concrete in cold-climate engineering applications. Full article

Anthropogenic activities interact through multifactorial processes that generate harmful factors, hindering wastewater management (WWM) and causing environmental degradation, particularly in rapidly urbanizing coastal regions. Understanding how these processes operate is essential for identifying effective interventions under data-limited conditions. This study introduces a process-based and context-driven approach whose main contribution lies in the construction of semantic pathways that represent how indirect anthropogenic drivers give rise to direct harmful factors affecting WWM and the environmental state. Semantic networks are used as a formal representation tool to model these pathways, where nodes represent factors and directed arcs represent causal relationships. Harmful semantic pathways are evaluated within a multidisciplinary decision-making framework supported by multi-criteria decision-making (MCDM) methods that account for environmental, social, economic, and sustainability criteria. The approach is applied to a coastal tourist municipality on the Mexican Pacific coast, where rapid urban expansion and insufficient basic services have severely constrained wastewater management and contributed to environmental damage. Results show that constructing and analyzing semantic pathways enables decision-makers to identify critical harmful factors and prioritize viable pro-environmental actions. The resulting priorities (range 0–1) highlight the restoration of wastewater treatment plants and improved urban planning as the most effective interventions, followed by mangrove reforestation and changes in agricultural practices. The proposed approach supports transparent, context-sensitive decision-making and is transferable to coastal tourist municipalities facing similar wastewater management challenges. Full article

Soil salinity is a major abiotic constraint on crop growth, yield, and quality. Advancing salt-tolerant agriculture requires an integrated understanding of salinity-induced osmotic stress, ionic toxicity, and oxidative damage, and how physiological and molecular networks regulate these processes. This review synthesizes key responses to salt stress, including osmotic adjustment, ion transport and compartmentalization, photosynthetic acclimation, reactive oxygen species detoxification, and phytohormone-mediated regulation by integrating mechanistic trial-level and deployment-focused insights while providing a fundamental translational insight into durable crop salt tolerance. We further summarize transcriptional and post-transcriptional control mechanisms involving stress-responsive genes, transcription factor families, and microRNA/non-coding RNA regulation. Finally, we compare progress and constraints in conventional breeding, transgenic approaches, genome editing, and epigenetic strategies for improving salinity tolerance and highlight priorities for translating these mechanistic insights into durable field performance. Full article

Background/Objectives: This study investigated the systemic metabolic effects of two probiotic strains, Lacticaseibacillus paracasei MG5012 and Bifidobacterium animalis subsp. lactis MG741, on metabolic dysfunction-associated steatotic liver disease (MASLD) and obesity-related muscle dysfunction in high-fat-diet (HFD)-induced obese mice. Methods: Obesity was induced in C57BL/6 mice via high-fat diet (HFD) feeding for 6 weeks. Subsequently, the mice were orally administered MG5012 or MG741 for 8 weeks. We assessed systemic metabolic parameters, including body weight, adiposity, and serum biomarkers. Additionally, histological and molecular analyses were performed to evaluate hepatic steatosis, intestinal barrier integrity, and muscle oxidative status. Results: Both strains significantly attenuated body weight gain and adiposity, reduced serum liver injury markers (γ-GTP, ALT, AST), and improved systemic metabolic parameters by restoring serum GLP-1 levels and reducing hyperinsulinemia. Crucially, MG5012 and MG741 strengthened intestinal barrier integrity by upregulating the tight junction proteins Occludin and Claudin-1. In the liver, histological analyses revealed reductions in hepatic steatosis and triglyceride content, accompanied by the downregulation of lipogenic genes (SREBP-1c, FAS). Furthermore, the probiotics preserved skeletal muscle integrity; while muscle weight remained unchanged, the strains increased muscle fiber cross-sectional area (CSA) and reduced serum markers of muscle damage (CPK, LDH). This protective effect was associated with significantly enhanced expression of antioxidant enzymes (SOD, CAT, GPx) in muscle tissue. Conclusions: These findings suggest that MG5012 and MG741 confer systemic metabolic benefits through the modulation of the gut–liver–muscle axis and may serve as promising functional food ingredients for the management of MASLD and obesity-associated muscle atrophy. Full article

Fiber Bragg Gratings (FBGs) are essential components in fiber-optic sensing systems owing to their high sensitivity, compact structure, and immunity to electromagnetic interference, and have been widely applied in structural health monitoring, aerospace, energy, and biomedical fields. Conventional FBG fabrication methods, including standing-wave, two-beam interference and phase mask methods, rely heavily on the photosensitivity of optical fibers and are limited in terms of fabrication flexibility and grating structural diversity. Femtosecond Laser Direct Writing (FLDW) has emerged as a prospective approach for FBG fabrication due to its nonlinear absorption mechanism, low thermal damage, three-dimensional processing capability and broad material compatibility. This review summarizes recent progress in FLDW-FBGs, with particular emphasis on the characteristics of point-by-point (PbP), line-by-line (LbL) and plane-by-plane (Pl-by-Pl) methods. The implementation of these methods in various fiber, including standard single-mode fibers, sapphire fibers, and polymer optical fibers, is discussed in detail. In addition, recent advances in FBG-based sensing applications under extreme environments, as well as in biomedical sensing and optical fiber communication, are reviewed. Key challenges related to fabrication efficiency, process stability, and microstructural characterization are further analyzed. Finally, potential development directions toward improved controllability, structural design flexibility, and engineering applicability of FLDW-FBGs are outlined. Full article

Background: Although the definition of dietary fibre is complex and constantly evolving, today we can identify it as “carbohydrate polymers with at least 10 monomeric units, which are not hydrolysed in the small intestine of humans”. In addition to the numerous and well-known benefits of dietary fibre for human health, our attention is drawn to its antioxidant properties, achieved through polyphenolic compounds linked to polysaccharide complexes. This study investigated the antioxidant effects of an extract from the fruit of Punica granatum (PUN), particularly rich in polyphenols, fibre, flavonoids, vitamins, organic acids, minerals, amino acids, and alkaloids. Furthermore, these effects were evaluated in two human nervous system cell lines under oxidative stress induced by hydrogen peroxide. Methodology: After examining the fibre composition, some polyphenols present in the extract were identified and quantified by HPLC. Furthermore, the antioxidant power of PUN was measured using the DPPH method, the chelating activity assay, the reducing power test, the ORAC method, the measurement of reactive oxygen species accumulation, the quantification of lipid peroxidation, and the detection of mitochondrial superoxide in cell cultures. Results: The results were consistent, and PUN demonstrated a strong antioxidant potential, justified not only by the high content of easily extractable polyphenols (EPPs) but also by a further addition of these more difficult to identify compounds (NEPPs), indicated as “hidden polyphenols”; therefore, the total polyphenol content in the extract resulted from the sum of EPPs + NEPPs (71 ± 7.9 + 55 ± 6.4 mg = 126 ± 14.3 mg gallic acid equivalent (GAE)/g dry weight). The fraction of hidden polyphenols could therefore explain a mechanism by which the fibre exerts an antioxidant effect. Another important result was achieved by the cell lines used, both of which were significantly protected by PUN following oxidative damage generated by a pro-oxidant treatment. However, astrocytes were found to be more responsive and sensitive than were human neurons. At the same time, PUN mitigated the effects of oxidative damage, and it could be hypothesised that this extract could be used to extinguish the A1 phenotype. Conclusions: We can conclude that the fibrous component of pomegranate is related to the antioxidant property exerted, and the neurodegeneration caused by oxidative stress could be slowed following the intake of Punica granatum. It is possible to identify the pomegranate as a “superfood” or “functional food”, with excellent nutritional characteristics and chemical composition. Full article

Glaucoma is no longer viewed solely as a pressure-mediated optic neuropathy but as a chronic neurodegenerative disease with a strong immune component. Across experimental models and patient samples, convergent inflammatory circuitry complement activation, NLRP3 inflammasome signaling, and microglial reactivity emerge as a central driver of retinal ganglion cell (RGC) dysfunction and death. Local complement upregulation (C1q, C3, C5) in the retina and optic nerve head (ONH) promotes aberrant synaptic tagging, phagoptosis, and membrane attack complex stress. In parallel, biomechanical strain, ischemia, mitochondrial damage, and danger-associated molecular patterns prime and activate the NLRP3 inflammasome in microglia, astrocytes, and ONH cells, leading to caspase-1 activation, IL-1β/IL-18 maturation, and pyroptotic or apoptotic injury. Microglia integrate these cues, shifting from early protective surveillance to chronic maladaptive states that amplify complement and inflammasome outputs. This review synthesizes mechanistic links within the complement NLRP3 microglia axis, considers systemic and adaptive immune contributions, and proposes a translational framework for immune-based clinical stratification. The literature for this review was identified through searches of PubMed, Web of Science, and Scopus using combinations of the terms ‘glaucoma’, ‘complement’, ‘inflammasome’, ‘NLRP3’, ‘microglia’, and ‘neuroinflammation’. Priority was given to recent experimental, translational, and clinical studies. We then evaluate emerging immunomodulatory therapies, complement inhibitors, inflammasome blockers, microglial state reprogrammers, cytokine biologics, and cell-derived immunoregulatory approaches, highlighting biomarkers and trial design needs. An immune systems view of glaucoma enables precision neuroprotection for patients who progress despite controlled intraocular pressure. Full article

Oxidative stress-induced endometrial injury has been shown to contribute to infertility; however, effective strategies that can simultaneously scavenge reactive oxygen species (ROS) and restore mitochondrial and antioxidant homeostasis remain elusive. In this study, we isolated extracellular nanovesicles from Artemisia argyi (A-NVs) and investigated their protective effects on H₂O₂-damaged human endometrial stromal cells (hESCs). We discovered that A-NVs possess a typical lipid bilayer structure and contain a variety of bioactive components. Our metabolomic analysis indicates that A-NVs can be regarded as a “natural drug reservoir”, in which flavonoids account for approximately 10.8%. We demonstrate that A-NVs can be efficiently taken up by cells, improve cell viability, reduce intracellular and mitochondrial ROS levels, enhance superoxide dismutase (SOD) activity, upregulate the expression of catalase (CAT), SOD1, and SOD2, and partially restore mitochondrial membrane potential. Mechanistically, A-NVs exert antioxidant effects by activating the SIRT1/PGC-1α/Nrf2 signaling axis. SIRT1 activation further alleviates H₂O₂-induced premature senescence, as evidenced by a 71.8% reduction in SA-β-Gal-positive cells compared with the H₂O₂ group, together with downregulation of p53 and p21 expression. These positive protective effects can be blocked by the SIRT1 inhibitor EX-527, confirming the central role of this pathway. Collectively, our findings demonstrate that A-NVs can maintain redox and mitochondrial homeostasis while inhibiting oxidative stress-related senescence progression, underscoring their application potential in endometrial repair and functional recovery. Full article