Search Results (42,138)

Medieval Islamic bustān gardens in the western Mediterranean played a crucial role in preserving and enriching rose diversity through the cultivation of species from the eastern Mediterranean and western Asia. These gardens, particularly in Al-Andalus, maintained distinctive rose varieties characterized by diverse flower morphology—ranging from white to deep crimson and near-black hues, including various yellow shades—and complex fragrance profiles with multiple olfactory nuances. The botanical heritage from these medieval Islamic gardens demonstrates remarkable persistence, with several of these cultivated rose species still found today in abandoned cortijos and aldeas throughout the mountains of eastern Spain. This study examines the transmission of rose culture through medieval Islamic bustān gardens, analyzing how these gardens served as repositories for ancient cultivars while introducing new varieties from eastern regions. Through examination of historical texts, iconographic evidence, and field documentation of surviving populations, we trace the continuity of medieval Islamic rose cultivation practices and their lasting impact on the rural landscape of eastern Spain. Flower scent is prominent as the leading factor determining preferences for medieval heritage rose cultivars, together with color and shape. The survival of these roses in abandoned settlements provides unique insight into the durability of medieval horticultural systems and the adaptation of cultivated species to semi-wild conditions over centuries. Full article

The precipitation of minerals, in particular carbonates, is a widespread phenomenon in all ecosystems, where it assumes a high relevance both from a geological and biogeochemical standpoint. Most carbonate rocks are of biological origin and made in an aquatic environment. In particular, bioprecipitation of carbonates is believed to have started in the Mesoproterozoic Era, thanks to a process often driven by photosynthetic microorganisms. Nevertheless, an important contribution to carbonate precipitation is also due to the metabolic activity of heterotrophic bacteria, which is not restricted to specific taxonomic groups or to specific environments, making this process a ubiquitous phenomenon. In this framework, the relationship between carbonatogenic microorganisms and other living organisms assumes a particular interest. This study aims to isolate and identify the culturable heterotrophic bacterial component associated with the coenosarc of Corallium rubrum in order to evaluate the occurrence of strains able to precipitate carbonates. In particular, the study was focused on the identification and characterisation of bacterial strains isolated from a coral coenosarc showing a high carbonatogenic capacity under laboratory conditions. Samples of C. rubrum were taken in the coastal waters of three Italian regions. The concentration of the aerobic heterotrophic microflora colonising C. rubrum coenosarc samples spanned from 3 to 6∙10⁶ CFU/cm². This variation in microbial populations colonising the C. rubrum coenosarc, spanning over 6 orders of magnitude, is not mirrored by a corresponding variability in the colony morphotypes recorded, with the mean being 5.1 (±2.1 sd). Among these bacteria, the carbonatogenic predominant species was Staphylococcus equorum (93% of the isolates), whereas Staphylococcus xylosus and Shewanella sp. accounted only for 3% of isolates each. All these strains showed a remarkable capacity of precipitating calcium carbonate, in the form of calcite crystals organised radially as well crystalised spherulites (S. equorum) or coalescing spherulites (Shewanella sp.). S. xylosus only produced amorphous precipitates of calcium carbonate. All bacterial strains identified were positive both for the production of urease and carbon anhydrase in vitro at 30 °C. It seems that they potentially possess the major biochemical abilities conducive to Ca²⁺ precipitation, as they showed in vitro. In addition, all our carbonatogenic isolates were able to hydrolyse the phytic acid calcium salt and then were potentially able to induce precipitation of calcium phosphates also through such a mechanism. Full article

Primary ciliary dyskinesia (PCD) is a genetically heterogeneous disorder characterized by impaired mucociliary clearance due to defects in motile cilia. This study investigates the impact of loss-of-function mutations in the CFAP300 gene on the ciliary structure and function in three PCD patients. Using a multimodal approach, we integrated molecular genetic testing, transmission electron microscopy, the high-speed video microscopy assay and immunofluorescence staining to analyze ciliary motility and protein expression in both ex vivo and in vitro-obtained ciliary cells. Our results revealed that the pathogenic variant c.198_200delinsCC (p.Phe67ProfsTer10) in CFAP300 led to the absence of the functional CFAP300 protein, the complete loss of outer and inner dynein arms and immotile cilia. Air–liquid interface (ALI)-cultured cells from patients exhibited no ciliary beating, contrasting with healthy controls. Immunostaining confirmed the absence of CFAP300 in patient-derived cilia, underscoring its critical role in dynein arm assembly. These findings highlight the diagnostic utility of ALI cultures combined with functional and protein analyses for PCD, offering a clinically actionable framework that can be readily incorporated into standard diagnostic workflows. Full article

Radegund of Poitiers (520–587) was a princess of the Thuringian kingdom, wife to the Merovingian king Clothar I, and ultimately domina of the abbey of Sainte-Croix in Poitiers. The literary persona of Saint Radegund, as constructed by the poet-hagiographer Venantius Fortunatus and, a few years later, by the nun Baudonivia, underpins the historical figure. The saint exerted a significant cultural influence across Frankish territories, and over the ages her image has been continuously received, reinterpreted, and expanded. The purpose of this study is to provide a survey of the critical reception of Radegund’s character, in order to explore how modern scholarship has interpreted and reimagined her persona over time. Full article

This study investigates the Enzyme Biofilm Method (EBM), a biological wastewater treatment technology previously developed by the authors. EBM employs microbial-derived hydrolytic enzyme groups in the initial treatment stage to break down high-molecular-weight organic matter—such as starch, proteins, and fats—into low-molecular-weight compounds. These compounds enhance the growth of native microorganisms, promoting biofilm formation on carriers and improving treatment efficiency. Over the past decade, EBM has been practically applied in food factory wastewater facilities handling high organic loads. The enzyme groups used in EBM are derived from cultures of Bacillus mojavensis, Saccharomyces cariocanus, and Lacticaseibacillus paracasei. To clarify the system’s mechanism and ensure its practical viability, this study focused on starch—a prevalent and recalcitrant component of food wastewater—using two evaluation approaches. Verification 1: Field testing at a starch factory showed that adding enzyme groups to the equalization tank effectively reduced biological oxygen demand (BOD) through starch degradation. Verification 2: Laboratory experiments confirmed that the enzyme groups possess both amylase and maltase activities, sequentially breaking down starch into glucose. The resulting glucose supports microbial growth, facilitating biofilm formation and BOD reduction. These findings confirm EBM’s potential as a sustainable and effective solution for treating high-strength food industry wastewater. Full article

Barn districts—ensembles of agricultural buildings situated at the edges of rural settlements—once played a key role in the spatial and economic organization of agrarian communities in Central Europe. Today, many of these structures remain marginalized and underexplored in contemporary landscape and heritage planning. This paper presents a comparative study of six barn districts in Poland’s Kraków-Częstochowa Upland, where vernacular construction, ecological adaptation, and local tradition shaped distinctive rural–urban interfaces. We applied a mixed-methods approach combining cartographic and archival analysis, field surveys, and interviews with residents and experts. The research reveals consistent patterns of landscape transformation, functional decline, and latent adaptive potential across varied morphological and material typologies. Despite differing levels of preservation, barn districts retain symbolic, spatial, and socio-cultural value for communities and local landscapes. The study emphasizes the importance of reintegrating these marginal heritage structures through adaptive reuse strategies rooted in the values of the New European Bauhaus—sustainability, aesthetics, and inclusion. The findings contribute to broader discussions on rural socio-ecological resilience and landscape-based development, highlighting how place-based strategies can bridge past identities with future-oriented spatial planning. Full article

Contemporary muralism has gained increasing cultural and social relevance in recent years, becoming a prominent form of urban artistic expression. However, its outdoor exposure makes it highly vulnerable to environmental degradation, raising significant challenges for long-term preservation. While solar radiation is widely recognized as a main agent of deterioration, the impact of rainfall has received comparatively little attention. This study addresses this gap by evaluating the durability of commercial protective coatings applied to modern paints (alkyd, acrylic, and styrene-acrylic) under simulated rain exposure. The ageing protocol replicates approximately 10 years of cumulative rainfall in Central-Southern Europe. A key innovation of this research is the use of a custom-built rain chamber, uniquely designed to expose a large number of samples simultaneously under highly uniform and controlled rain conditions. The system ensures reproducible exposure through a precision-controlled moving platform and programmable rain delivery. A comprehensive set of analytical techniques was employed to assess morphological, chemical, and functional changes in the coatings and paints before and after ageing. Results highlight the limited performance of current protective materials and the need for more effective solutions for the conservation of contemporary outdoor artworks. Full article

Fungi of the genus Trichoderma show strong potential as biological control agents (BCAs) against grapevine trunk diseases (GTDs) through mechanisms like antibiotic metabolite production and lytic enzymes. This study evaluated the biocontrol activity of four native Trichoderma strains—T. gamsii T065 and T071, T. carraovejensis T154, and T. harzianum T214—against Phaeoacremonium minimum, Phaeomoniella chlamydospora, and Diplodia seriata. Culture filtrates obtained at 8, 16, and 24 days post-incubation were tested using antibiogram and mycelial inhibition assays. Strains T071, T154, and T214 effectively inhibited D. seriata, while T154 and T214 also suppressed P. chlamydospora. Nevertheless, the limited effectiveness of all filtrates against P. minimum suggests that antibiosis is not the predominant mechanism involved in its control. These findings highlight the potential of specific Trichoderma strains and incubation times to directly control GTD pathogens and support the development of scalable biocontrol solutions. Full article

Background: Exposure-based cognitive behavioral therapy (Ex-CBT) is widely seen as the gold-standard treatment for anxiety and obsessive-compulsive disorder (OCD). Yet, minoritized youth are underrepresented in efficacy studies, raising questions about the applicability of Ex-CBT to minoritized youth. Effectiveness data suggest systematic adaptation of Ex-CBT to address youth culture and context is likely needed, and many clinicians make adaptations and augmentations in practice. However, research on the specific strategies clinicians use to address their youth clients’ culture and context within anxiety and OCD treatment is lacking. In the current study, we assess practice-based adaptations, augmentations, and process-based approaches utilized when delivering treatment to youth for OCD and anxiety in public mental health clinics. Methods: We conducted qualitative interviews with 16 clinicians from both specialty anxiety and general mental health clinics serving youth with anxiety or OCD in the public mental health system. Participating clinicians had a mean age of 32.19 (SD = 5.87) and 69% of therapists identified as female; 69% identified as White, 25% identified as Asian, and 6% as Black or African American. In qualitative interviews, clinicians shared how they addressed clients’ culture and context (e.g., social identities, stressors and strengths related to social identities and lived environment). Thematic analysis identified the strategies clinicians employed to address culture and context. Results: Clinicians reported incorporating culture and context through process-based approaches (e.g., building trust gradually, considering clients’ social identity stressors, engaging in self-awareness to facilitate cultural responsiveness) and through culturally adapting and augmenting treatment to promote person-centered care. Core strategies included proactive and ongoing assessment of clients’ cultural and contextual factors, adapting exposures and augmenting Ex-CBT with strategies such as case management and discussion of cultural context, and taking a systems-informed approach to care. Conclusions: Examining practice-based adaptations, augmentations, and process-based approaches to treatment for minoritized youth with OCD or anxiety can inform efforts to understand what comprises person-centered culturally responsive Ex-CBT. Empirical testing of identified strategies is a needed area of future research. Full article

Background: Midwifery’s Intangible Heritage was officially recognized by the United Nations Educational, Scientific and Cultural Organization on 6 December 2023, highlighting that elements of midwifery knowledge and practice, shaped over generations, constitute cultural heritage worth safeguarding. While previous studies have investigated midwives’ perceptions of professional identity, none have done so within the explicit framework of MIH. Objective: this study explored how midwives and student midwives in Cyprus perceive the intangible heritage of their profession and how it relates to their shared professional identity. Methods: A qualitative descriptive study was conducted between April and July 2023. Three focus groups were held, involving 22 participants: 15 registered midwives and 7 student midwives. A semi-structured interview guide consisting of 10 questions was used, developed by the lead author (M.P.) based on the literature and improvisation and finalized with the research team (E.H., S.V., N.M.) after expert input. Thematic analysis was performed inductively to identify recurrent themes. Results: Four major themes emerged: (1) key elements of Midwifery’s Intangible Heritage and their transmission across generations; (2) a sense of shared professional identity; (3) perceived threats to the midwifery profession; and (4) midwives’ expectations for the future of the profession. Conclusions: The findings reflect the historical background of midwifery in Cyprus and its contrast with contemporary practice, particularly within the context of the overmedicalization of birth and societal perceptions of midwifery in the socio-cultural setting. Safeguarding Midwifery’s Intangible Heritage requires both empowering women to seek midwifery-led care and enabling midwives to practice autonomously within their full professional scope. In addition, policymakers and educational bodies must support the preservation of midwives’ core skills through targeted educational curricula, structured mentorship, and continuous professional development. Full article

Archaeological research fundamentally relies on detecting features to uncover hidden historical information. Airborne (aerial) LiDAR technology has significantly advanced this field by providing high-resolution 3D terrain maps that enable the identification of ancient structures and landscapes with improved accuracy and efficiency. This technical note comprehensively reviews 45 recent studies to critically examine the integration of Machine Learning (ML) and Deep Learning (DL) techniques, particularly Convolutional Neural Networks (CNNs), with airborne LiDAR derivatives for automated archaeological feature detection. The review highlights the transformative potential of these approaches, revealing their capability to automate feature detection and classification, thus enhancing efficiency and accuracy in archaeological research. CNN-based methods, employed in 32 of the reviewed studies, consistently demonstrate high accuracy across diverse archaeological features. For example, ancient city walls were delineated with 94.12% precision using U-Net, Maya settlements with 95% accuracy using VGG-19, and with an IoU of around 80% using YOLOv8, and shipwrecks with a 92% F1-score using YOLOv3 aided by transfer learning. Furthermore, traditional ML techniques like random forest proved effective in tasks such as identifying burial mounds with 96% accuracy and ancient canals. Despite these significant advancements, the application of ML/DL in archaeology faces critical challenges, including the scarcity of large, labeled archaeological datasets, the prevalence of false positives due to morphological similarities with natural or modern features, and the lack of standardized evaluation metrics across studies. This note underscores the transformative potential of LiDAR and ML/DL integration and emphasizes the crucial need for continued interdisciplinary collaboration to address these limitations and advance the preservation of cultural heritage. Full article

Background: Patient safety culture is critical for enhancing the quality and safety of healthcare. Studies in low- and middle-income countries have reported challenges in developing patient safety culture, especially in implementing nonpunitive responses to errors and event reporting. However, evidence from Laos remains limited. Objectives: This study aimed to assess patient safety culture in hospitals in southern Laos, using a validated survey tool to identify strengths and areas of improvement. Methods: A cross-sectional study using purposive sampling was conducted in four provincial and twenty-three district hospitals in southern Laos. Healthcare workers on patient safety committees responded to the Hospital Survey on Patient Safety Culture. The positive response rate was analyzed. Bivariate tests (chi-square/Fisher’s exact) were applied to compare positive response rates between hospital types and professions. Results: A total of 253 valid responses (75.5%) were analyzed. “Organizational Learning–Continuous Improvement” scored over 75% in both provincial and district hospitals. In contrast, “Nonpunitive Response to Error” and “Frequency of Events Reported” were scored <20% on average. Provincial hospitals scored significantly higher than district hospitals in supervisory support and handoffs. Conclusions: This study illustrated strengths in organizational learning while identifying nonpunitive responses and event reporting as critical areas of improvement for hospitals in Laos. To improve patient safety, hospitals in Laos must promote a culture in which errors can be reported without fear of blame. Strengthening leadership support and reporting systems is essential. These findings can inform strategies to enhance patient safety in other low-resource healthcare settings. Full article

Gender-neutral terms such as “sex work” and “sex worker” have gained prominence in academic, activist, and policy discourses. Unlike terms such as ‘prostitutes’ and ‘prostitution,’ these terms serve to reduce stigma and emphasize the labor involved in sex work. However, numerous studies suggest that these terms carry different meanings across cultural contexts, and their impact is experienced differently by various sub-groups of sex workers. Although originally coined by American activist Carol Leigh to unify sex workers across genders and sectors, these terms are, in some settings—including Kolkata, India—met with silence, coded language, or secrecy, particularly among transient sex workers, including cisgender and transgender women. As researchers with two decades of ethnographic research with 46 cisgender and 37 transgender women engaged in sex work in Kolkata and Eastern India, the authors argue that such silence and non-verbal forms of communication should not be interpreted merely as ignorance or disengagement but rather as strategic/willful acts of agency or resistance. These practices enable sex workers situated in different contexts to navigate unequal power dynamics within NGOs, manage respectability, mitigate structural violence, and foster communal identity. By examining how diverse groups of sex workers employ silence and secrecy, this study contributes to a more nuanced and empathetic understanding of the complexities surrounding the former’s lives and their agency. The study results indicate that it is essential for scholars, advocates and activists to employ strengths-based, context-specific language and research practices to be able to hear the stories of subaltern groups like commercial sex workers. It is also suggested that training of service providers and helping professionals focus on accepting and honoring the language people use to identify themselves in varied spaces and contexts. Full article

The market for plant-based alternatives to animal foods has increased rapidly in the past decade, mainly due to consumer demand. Little evidence is available regarding nutritional impacts, drivers, and barriers to using these products as substitutes for animal foods in real-life conditions. This pilot study followed 16 Danish adults (30 ± 11 years old; 11 females) for 4 weeks with substituting milk, cheese, and yogurt with plant-based analogues to dairy (PBADs) and assessed their drivers and barriers to applying the intervention with a mixed-method approach. PBADs are constantly compared to their animal counterparts, both regarding product characteristics, such as price and sensory properties, as well as cultural roles and subjective memories. The mixed methods showed dairy attachment, price, and taste were the main barriers to consuming PBAD, while changes in life and social circles were drivers (qualitative data). As for the liking of PBADs, plant-based yoghurt was the preferred intervention product (73.5/100, p < 0.05), followed by plant-based drinks (65.9/100), while plant-based cheese was the lowest rated (47.9/100, p < 0.05). As for dietary changes, a lower average intake of sugars, saturated fatty acids, cholesterol, calcium, phosphorus, and zinc was observed after the intervention. Additionally, this study describes the attachment of the study population to milk and dairy products. It shows that choosing dairy is beyond nourishment but is connected to tradition, culture, pleasure, memories, and a sense of belonging. In contrast, there is no history or attachment to PBADs. Full article

Spain is among the European countries with the greatest number of preserved castles and defensive structures—some estimates place the total at around 10,000, the majority of which date back to the medieval period. Yet, surprisingly, many of these fortifications remain uncatalogued and in an advanced state of ruin. This study focuses on a small fortress that has been overlooked by historiography and neglected by public authorities, yet which still stands after seven centuries: the Tower of Lopera, a castle belonging to the so-called Banda Morisca (the frontier of Al-Andalus in the 14th century). Using a combination of digital documentation techniques—namely, portable laser scanning, photogrammetry (via drone and camera), and digital image processing software—we have been able to digitize, geometrize, and document both the surviving architectural remains and their immediate physical environment. Rather than pursuing the latest technological innovations, this methodology prioritizes practical and realistic solutions based on the resources typically available to cultural heritage administrations. Our work serves two main objectives: to demonstrate the viability of applying such tools to this typology of architectural heritage and to conduct a detailed graphic and geometric analysis of the structure. Given the abundance of similar abandoned fortresses in Spain, the findings presented here could inform future heritage documentation strategies on a broader, potentially national, scale. Full article