Search Results (3,278)

The practice in agriculture of spreading polyethylene (PE) film over the soil surface as mulch is a common, global practice that aids in conserving water, increasing crop yields, suppressing weed growth, and decreasing growing time. However, these films are typically only used for a single growing season, and thus, their use and non-biodegradability come with some serious environmental consequences due to their persistence in the soil and potential for microplastic pollution, particularly when retrieval and disposal options are poor. On the microscale, particles < 5 mm from degraded films have been observed to disrupt soil structure, impede water and nutrient cycling, and affect soil organisms and plant health. On the macroscale, there are obvious and serious environmental consequences associated with the burning of plastic film and its leakage from poorly managed landfills. To maintain the crop productivity afforded by mulching with PE film while avoiding the environmental downsides, the development and use of biodegradable polymer technologies is being explored. Here, the efficacy of a newly developed, water-dispersible, sprayable, and biodegradable polyester–urethane–urea (PEUU)-based polymer was compared with two commercial PE mulches, non-degradable polyethylene (NPE) and OPE (ox-degradable polyethylene), in a greenhouse tomato growth trial. Water savings and the effects on plant growth and soil characteristics were studied. It was found that PEUU provided similar water savings to the commercial PE-based mulches, up to 30–35%, while showing no deleterious effects on plant growth. The results should be taken as preliminary indications that the sprayable, biodegradable PEUU shows promise as a replacement for PE mulch, with further studies under outside field conditions warranted to assess its cost effectiveness in improving crop yields and, importantly, its longer-term impacts on soil and terrestrial fauna. Full article

In most countries, liver disease is one of the most common pathologic conditions among the population. In this regard, the development of new methods to treat liver diseases is not possible without understanding the mechanisms of regeneration of this organ. A characteristic reaction of the liver to certain damaging factors is a pronounced cellular plasticity; this primarily concerns hepatocytes and cholangiocytes. This property is also characteristic of Ito stellate cells and macrophages. In this study, we focus on the plasticity of hepatocytes and cholangiocytes. We consider such manifestations of plasticity as the ability to enter the mitotic cycle, as well as transdifferentiation. The contribution of each type of plasticity to liver regeneration is considered, as well as the molecular mechanisms providing the cellular plasticity of hepatocytes and cholangiocytes. Full article

Background: Conventional practices for the cultivation of “Cipollotto Nocerino” spring onion are mainly based on growers’ experience, and up to 250 kg/ha for N is commonly furnished among growing cycles. Facing the issue of reduced availability of natural resources for crop production (for example mineral resources), we investigated the optimization of the productivity. Methods: In our research, we tested the use of depletion nutrient supply strategy (CAL-FERT^®) to enhance fertilization in accordance with the principle of sustainable agriculture included in the Farm to Fork strategy. In our study, besides the common initial fertilization, three different strategies for cover fertilizations have been elaborated with the support of CAL-FERT^® software. The treatments were as follows: (i) commercial standard fertilization as control (named CF); (ii) fertilization equivalent to 50% of the N applied in the control (named F-50); (iii) fertilization corresponding to 25% of the N applied in the control (named F-25); and (iv) strongly reduced fertilization compared to the control (named F-0). The parameters investigated included the following: plant height, yield, SPAD index, nitrogen use efficiency, dry matter, soluble solid content, and pyruvate contents in bulbs and leaves. Nitrogen content was also analyzed for both hypogeous and epigeous apparatuses. Results: Among the most interesting vegetative results, plant height and SPAD readings were reduced only by the extreme treatment F-0 compared with the other treatments at 104 days after planting. Regarding qualitative and productive parameters, the treatments F-50 and F-25 showed the highest yield without prejudging Soluble Solid Content and reducing pungency. Conclusion: In nutritional experiments, onion could be considered as a crop model to investigate quality in vegetables due to its consumption as fresh product and for its particular response, in terms of yield and quality, to fertilization. The use of simulation software can support the identification of strategies to reduce the nutrient supply without any detrimental effect on yield and other vegetative and qualitative parameters in onion crops. Full article

In wet subtropical environments, perennial groundcovers are common in horticultural plantations to protect the soil from erosion. However, there has been little investigation into whether seeding annual cover crops into the perennial groundcovers provides additional soil services including carbon and nutrient cycling in these systems. To investigate this, farmer participatory field trials were conducted in commercial avocado, macadamia, and coffee plantations in the wet Australian subtropics. Cover crops were direct-seeded into existing inter-row groundcovers in winter (cool season cover crops), and into the same plots the following summer (warm season cover crops). Inter-row biomass was quantified at the end of winter and summer in the control (no cover crop) and cover crops treatments. Soil carbon and nutrient cycling parameters including hot water extractable carbon, water soluble carbon, autoclavable citrate-extractable protein and soil enzyme activities were quantified every two months from early spring (September) 2021 to late autumn (May) 2022. Seeded cover crops produced 500 to 800 kg ha⁻¹ more total inter-row biomass over winter at the avocado coffee sites, and 3000 kg ha⁻¹ biomass in summer at the coffee site. However, they had no effect on biomass production in either season at the macadamia site. Soil functional parameters changed with season (i.e., time of sampling), with few significant effects of cover crop treatments on soil function parameters across the three sits. Growing a highly productive annual summer cover crop at the coffee site led to suppression and death of perennial groundcovers, exposing bare soil in the inter-row by 3 weeks after termination of the summer cover crop. Annual cover crops seeded into existing perennial groundcovers in tree crop systems had few significant impacts on soil biological function over the 12-month period, and their integration needs careful management to avoid investment losses and exacerbating the risk of soil erosion on sloping lands in the wet subtropics. Full article

The growing imperative to mitigate climate change and accelerate the shift toward energy sustainability has called for a critical evaluation of heat and electricity generation methods. This article presents a comparative life cycle assessment (LCA) of solar and biogas energy systems on a common basis of 1 kWh of useful energy using SimaPro, the ReCiPe 2016 methodology (both midpoint and endpoint indicators), and cumulative energy demand (CED) analysis. This study is the first to evaluate co-located solar PV, solar thermal compound parabolic concentrator (CPC) and biogas combined heat and power (CHP) systems with in situ data collected under identical climatic and operational conditions. The project costs yield levelized costs of electricity (LCOE) of INR 2.4/kWh for PV, 3.3/kWh for the solar thermal dish and 4.1/kWh for biogas. However, the collaborated findings indicate that neither solar-based systems nor biogas technology uniformly outperform the others; rather, their effectiveness hinges on contextual factors, including resource availability and local policy incentives. These insights will prove critical for policymakers, industry stakeholders, and local communities seeking to develop effective, context-sensitive strategies for sustainable energy deployment, emissions reduction, and robust resource management. Full article

In this cross-disciplinary investigation, we uncover a suite of previously unexamined factors and their intricate interplay that hold causal relationships with the distribution of Trachurus japonicus in the northern reaches of the South China Sea, thereby extending the existing research paradigms. Leveraging advanced machine learning algorithms and causal inference, our robust experimental design uncovered nine key global and regional factors affecting the distribution of T. japonicus density. A robust experimental design identified nine key factors significantly influencing this density: mean sea-level pressure (msl-0, msl-4), surface pressure (sp-0, sp-4), Summit ozone concentration (Ozone_sum), F10.7 solar flux index (F10.7_index), nitrate concentration at 20 m depth (N3M20), sonar-detected effective vertical range beneath the surface (Height), and survey month (Month). Crucially, stable causal relationships were identified among Ozone_sum, F10.7_index, Height, and N3M20. Variations in Ozone_sum likely impact surface UV radiation levels, influencing plankton dynamics (a primary food source) and potentially larval/juvenile fish survival. The F10.7_index, reflecting solar activity, may affect geomagnetic fields, potentially influencing the migration and orientation behavior of T. japonicus. N3M20 directly modulates primary productivity by limiting phytoplankton growth, thereby shaping the availability and distribution of prey organisms throughout the food web. Height defines the vertical habitat range acoustically detectable, intrinsically linking directly to the vertical distribution and availability of the fish stock itself. Surface pressures (msl-0/sp-0) and their lagged effects (msl-4/sp-4) significantly influence sea surface temperature profiles, ocean currents, and stratification, all critical determinants of suitable habitats and prey aggregation. The strong influence of Month predominantly reflects seasonal changes in water temperature, reproductive cycles, and associated shifts in nutrient supply and plankton blooms. Rigorous robustness checks (Data Subset and Random Common Cause Refutation) confirmed the reliability and consistency of these causal findings. This elucidation of the distinct biological and physical pathways linking these diverse factors leading to T. japonicus density provides a significantly improved foundation for predicting distribution patterns globally and offers concrete scientific insights for sustainable fishery management strategies. Full article

The building sector remains one of the largest contributors to global energy consumption and CO₂ emissions, yet selecting optimal retrofit strategies is often hindered by inconsistent evaluation practices and limited integration of environmental and social impacts. This review addresses that gap by systematically analyzing how various assessment methods are applied to building retrofits, particularly from a financial and environmental perspective. A structured literature review was conducted across four major scientific databases using predefined keywords, filters, and inclusion/exclusion criteria, resulting in a final sample of 50 studies (green colored citations of this paper). The review focuses on the application of Life Cycle Cost Analysis (LCCA), Cost–Benefit Analysis (CBA), and Life Cycle Assessment (LCA), as well as additional indicators that quantify energy and sustainability performance. Results show that LCCA is the most frequently used method, applied in over 60% of the studies, often in combination with LCA (particularly for long time horizons). CBA appears in fewer than 25% of cases. More than 50% of studies are based in Europe, and over 60% of case studies involve residential buildings. EnergyPlus and DesignBuilder were the most common simulation tools, used in 28% and 16% of the cases, respectively. Risk and uncertainty were typically addressed through Monte Carlo simulations (22%) and sensitivity analysis. Comfort and social impact indicators were underrepresented, with thermal comfort included in only 12% of studies and no formal use of tools like Social-LCA or SROI. The findings highlight the growing sophistication of retrofit assessments post-2020, but also reveal gaps such as geographic imbalance (absence of African case studies), inconsistent treatment of discount rates, and limited integration of social indicators. The study concludes that future research should develop standardized, multidimensional evaluation frameworks that incorporate social equity, stakeholder values, and long-term resilience alongside cost and carbon metrics. Full article

The black-spotted frog (Pelophylax nigromaculatus) is a common economic species in the rice–frog ecological cropping mode. The present study investigated microbial community structures in paddy water and black-spotted frog’s guts across rice monoculture and low-/high-density rice–frog co-cropping systems at four rice growth stages. Proteobacteria dominate in paddy water, while frog guts are enriched in Firmicutes and Actinobacteriota. The frog density shows no impact on the α-diversity, but rice growth stages significantly alter the Shannon, Simpson, and Pielou_e indices (p < 0.01). Co-cropping may promote amino acid synthesis, elemental cycling, and stress tolerance in paddy water microbiota, which are more diverse than gut microbiota. Strong correlations exist between paddy water and gut microbiotas, with Limnohabitans being linked to gut diversity (p < 0.05). Low-density co-cropping enhances Xenorhabdus, which is beneficial for pest control and stabilizes gut microbiota. The results of this study offer insights for managing rice–frog systems based on rice growth stages. Full article

Understanding how sample type may influence the probability of influenza A virus (IAV) sequencing and isolation success can help improve the use of diagnostic tests and refine surveillance strategies in swine populations. The objective of this study was to evaluate the probability of success for IAV hemagglutinin (HA) and neuraminidase (NA) Sanger sequencing and virus isolation in Madin–Darby Canine Kidney (MDCK) cells across different porcine sample types submitted to the Iowa State University Veterinary Diagnostic Laboratory (ISU VDL) from 2018 to 2024. Antemortem and postmortem sample types were selected and analyzed based on reverse transcription real-time polymerase chain reaction (RT-rtPCR) cycle threshold (Ct) values. The Ct values corresponding to 95%, 75%, and 50% probabilities of sequencing or virus isolation success were determined for each sample type. For antemortem samples, a 95% probability of success for HA Sanger sequencing on nasal swabs exhibited a Ct value of 27.8 from 1046 samples and 23.6 for NA sequencing based on 66 nasal swabs. Using oral fluids, HA and NA Sanger sequencing success was at Ct values of 27.3 from 3446 samples and 22.1 from 137 samples, respectively. For postmortem samples, lung tissue had the highest number of sequences for the HA and NA, with Ct values of 25.7 and 21.5, respectively. For a 95% probability of successful virus isolation, nasal swabs demonstrated a Ct value of 21.1 from 647 samples, while lungs had a Ct value of 18.7 from 5892 samples. This study determined that nasal swabs and lung tissue had the highest probability of IAV gene sequencing and virus isolation success, while oral fluids, a common swine diagnostic sample type that is easy to collect and welfare-friendly, can be effective for gene sequencing when using lower IAV RT-rtPCR Ct values, i.e., ≤27.3. These results provide practical expectations for successful IAV HA and NA gene sequencing and virus isolation at 95%, 75%, and 50% probabilities based on sample type and RT-rtPCR Ct values to improve diagnostic testing strategies in swine populations. Full article

Exercise is an important component in the treatment and improvement of function in populations with or at risk of lower limb injury. Cycling is the most common exercise modality used by these populations. However, reduced lower limb joint excursion and/or range of motion (ROM) during cycling might limit the optimization of functional improvements. Increased hip flexion gait (HFgait) is a new exercise modality that might result in larger lower limb joint excursions compared to cycling. The purpose of this study was to compare lower limb kinematics between HFgait and cycling. Twelve healthy individuals participated in the study. Each participant performed cycling and HFgait. Hip, knee, and ankle kinematics in the sagittal, frontal, and transverse planes were analyzed with and without phase offset reduction (POR). Discrete and continuous analyses were performed. Discrete analysis indicated differences for at least one of the variables analyzed (maximum, minimum, and ROM) for the hip (p ≤ 0.041), knee (p ≤ 0.008), and ankle (p ≤ 0.040) across all planes. For the continuous analysis, differences between HFgait and cycling kinematics were observed during the cycles for the hip, knee, and ankle sagittal (hip: original: 85%; with POR: 77%; knee: original: 93%; with POR: 76%; ankle: original: 14%; with POR: 14%), frontal (hip: original: 93%; with POR: 98%; knee: original: 41%; with POR: 12%; ankle: original: 4%; with POR: 5%), and transverse (hip: original: 66%; with POR: 0%; knee: original: 14%; with POR: 0%; ankle: original: 3%; with POR: 0%) planes. HFgait resulted in larger hip (+60.2°) and knee (+38.2°) sagittal plane ROM while maintaining the hip in a more neutral position in the frontal plane compared with cycling. These findings can support the development of rehabilitation strategies with the goal of improving function and joint range of motion while also receiving the health benefits of exercise. Full article

Oncidium has an important market value, with important high-grade cut orchids and potted flowers on the flower market. In the Oncidium cut flowers production industry, there is a common phenomenon that the development of vegetative buds disrupts the normal generation cycle of the inflorescence induction, so-called “bud jumping”. In this study, vegetative bud differentiation and flower bud differentiation were divided into three stages, namely, the initial stage of differentiation, the leaf primordial/flower primordial differentiation stage, and the late stage of leaf bud/flower bud differentiation, as observed by paraffin sectioning. Secondly, we analyzed the differences between the vegetative buds of “bud jumping” plants and the flower buds of normal flowering plants by transcriptome sequencing. The transcriptome analysis results revealed significant differences among plant signaling pathways, particularly in gibberellins, auxins, and cytokinins, which play important roles in this phenomenon’s formation. In conjunction with the transcriptome analysis, the researchers conducted field experiments by applying plant growth regulators on the newborn pseudobulb of young Oncidium plants measuring approximately 49 mm in length. The results showed that the treatment groups of 100 mg/L of gibberellic acid (GA₃) and 100 mg/L GA₃ + 10 mg/L 6-Benziladenine (6-BA) exhibited the highest rate of flower bud differentiation instead of the least “bud jumping” phenomenon, and the “bud jumping” phenomenon was significantly reduced under 25 mg/L, 50 mg/L, and 75 mg/L 3-indoleacetic acid (IAA) treatments. The application of exogenous gibberellins, cytokinins, and auxins can effectively reduce the occurrence of “bud jumping”. Full article

Against the backdrop of ecological civilization construction and regional coordinated development strategies, functional zone (MFOZ) planning guides national spatial development through differentiated policies. However, a prominent conflict exists between the ecological protection responsibilities and regional development rights in restricted and prohibited development zones, leading to a vicious cycle of “ecological protection → restricted development → poverty exacerbation”. This paper focuses on the synergistic mechanisms between ecological compensation and targeted poverty alleviation. Based on the capability approach and sustainable development goals (SDGs), it analyzes the dialectical relationship between the two in terms of goal coupling, institutional design, and practical pathways. The study finds that ecological compensation can break the “ecological poverty trap” through the internalization of externalities and the enhancement of livelihood capabilities. Nevertheless, challenges remain, including low compensation standards, unbalanced benefit distribution, and insufficient legalization. Through case studies of the compensation reform in the water source area of Southern Shaanxi, China, and the Common Agricultural Policy (CAP) of the European Union, this paper proposes the construction of a long-term mechanism integrating differentiated compensation standards, market-based fund integration, legal guarantees, and capability enhancement. The research emphasizes the need for institutional innovation to balance ecological protection and livelihood improvement, promoting a transition from “blood transfusion” compensation to “hematopoietic” development, thereby offering a Chinese solution for global sustainable development. Full article

Pedicle screw fixation is a common spinal surgery technique, but concerns remain about stability when screws are malpositioned. Traditional in vitro pull-out tests assess anchorage but lack physiological accuracy. This study examined the stability of correctly placed and intentionally malpositioned pedicle screws on forty vertebrae from five cadavers. Optimal screw paths were planned via CT scans and applied using 3D-printed guides. Four malposition types—medial, lateral, superior, and superior-lateral—were created by shifting the original trajectory. A custom setup applied three consecutive cycles of tensile and compressive load from 50 N to 200 N. Screw inclination under load was measured with a 3D optical system. The results showed increasing screw inclination with higher forces, reaching about 1° at 50 N and 2° at 100 N, similar in both load directions. Significant differences in inclination were only found at 100 N tensile load, where malpositioned screws showed a lower inclination. Overall, malpositioning had no major effect on screw loosening. These findings suggest that minor deviations in screw placement do not significantly compromise mechanical stability. Clinically, the main concern with malpositioning lies in the potential for injury to nearby structures rather than reduced screw fixation strength. Full article

As a key component in the hydraulic brake system of automobiles, the brake joint directly affects the braking performance and driving safety of the vehicle. Therefore, improving the quality of brake joints is crucial. During the processing, due to the complexity of the welding material and welding process, the weld seam is prone to various defects such as cracks, pores, undercutting, and incomplete fusion, which can weaken the joint and even lead to product failure. Traditional weld seam detection methods include destructive testing and non-destructive testing; however, destructive testing has high costs and long cycles, and non-destructive testing, such as radiographic testing and ultrasonic testing, also have problems such as high consumable costs, slow detection speed, or high requirements for operator experience. In response to these challenges, this article proposes a defect detection and classification method for laser welding seams of automotive brake joints based on machine vision inspection technology. Laser-welded automotive brake joints are subjected to weld defect detection and classification, and image processing algorithms are optimized to improve the accuracy of detection and failure analysis by utilizing the high efficiency, low cost, flexibility, and automation advantages of machine vision technology. This article first analyzes the common types of weld defects in laser welding of automotive brake joints, including craters, holes, and nibbling, and explores the causes and characteristics of these defects. Then, an image processing algorithm suitable for laser welding of automotive brake joints was studied, including pre-processing steps such as image smoothing, image enhancement, threshold segmentation, and morphological processing, to extract feature parameters of weld defects. On this basis, a welding seam defect detection and classification system based on the cascade classifier and AdaBoost algorithm was designed, and efficient recognition and classification of welding seam defects were achieved by training the cascade classifier. The results show that the system can accurately identify and distinguish pits, holes, and undercutting defects in welds, with an average classification accuracy of over 90%. The detection and recognition rate of pit defects reaches 100%, and the detection accuracy of undercutting defects is 92.6%. And the overall missed detection rate is less than 3%, with both the missed detection rate and false detection rate for pit defects being 0%. The average detection time for each image is 0.24 s, meeting the real-time requirements of industrial automation. Compared with infrared and ultrasonic detection methods, the proposed machine-vision-based detection system has significant advantages in detection speed, surface defect recognition accuracy, and industrial adaptability. This provides an efficient and accurate solution for laser welding defect detection of automotive brake joints. Full article

Pseudomonas aeruginosa is a highly adaptable opportunistic pathogen with significant clinical relevance in both human and veterinary medicine. Despite its well-documented role in hospital-acquired infections in human healthcare settings, its persistence and transmission within veterinary clinics remain underexplored. This review highlights the overlooked status of veterinary facilities as environmental reservoirs and amplification points for multidrug-resistant (MDR) P. aeruginosa, emphasizing their relevance to One Health surveillance. We examine the bacterium’s environmental survival strategies, including biofilm formation, resistance to disinfectants, and tolerance to nutrient-poor conditions that facilitate the long-term colonization of moist surfaces, drains, medical equipment, and plumbing systems. Common transmission vectors are identified, including asymptomatic animal carriers, contaminated instruments, and the hands of veterinary staff. The review synthesizes current data on antimicrobial resistance in environmental isolates, revealing frequent expression of efflux pumps and mobile resistance genes, and documents the potential for zoonotic transmission to staff and pet owners. Key gaps in environmental monitoring, infection control protocols, and genomic surveillance are identified, with a call for standardized approaches tailored to the veterinary context. Control strategies, including mechanical biofilm disruption, disinfectant cycling, effluent monitoring, and staff hygiene training, are evaluated for feasibility and impact. The article concludes with a One Health framework outlining cross-species and environmental transmission pathways. It advocates for harmonized surveillance, infrastructure improvements, and intersectoral collaboration to reduce the risk posed by MDR P. aeruginosa within veterinary clinical environments and beyond. By addressing these blind spots, veterinary facilities can become proactive partners in antimicrobial stewardship and global resistance mitigation. Full article