Search Results (3,653)

Abiotic stresses increasingly threaten crop productivity, whereas reliance on chemical and resource-intensive interventions can compromise environmental sustainability. Existing literature identifies Trichoderma spp. as multifunctional biocontrol agents, biofertilizers, and microbial biostimulants capable of influencing plant growth, stress signaling, and rhizosphere processes; however, evidence remains fragmented across strains, crops, formulations, and stress conditions. This review aimed to integrate current knowledge on Trichoderma-mediated resilience to salinity, drought, heavy metals, temperature extremes, and emerging pollutants, while distinguishing experimentally validated mechanisms from statistical associations and conceptual inference. It evaluates constraints governing reproducibility from controlled studies to field deployment. The synthesis shows that selected crop–strain systems improve root architecture, photosynthesis, antioxidant regulation, osmotic adjustment, nutrient acquisition, ion homeostasis, hormonal balance, and stress-responsive gene expression. Benefits arise through coordinated delivery, root colonization, metabolite and protein signaling, physiological reprogramming, and rhizosphere modulation. Nevertheless, microbiome co-occurrence patterns do not establish causal network repair, evidence for broad heat and cold protection remains limited, and biochar co-application should not be interpreted as a carrier formulation without direct validation. Future progress requires strain- and crop-specific screening, mechanistic gene and protein studies, standardized formulations, combined-stress experiments, multi-location field trials, biosafety evaluation, and farmer-level economic assessment to develop reliable precision microbial technologies. Full article

The Fruit-Weight 2.2-Like (FWL) gene family plays crucial roles in determining organ size and stress responsiveness in plants. However, the FWL family members remain largely unexplored in Gossypium species. In this study, we identified and characterized the FWL family in Gossypium hirsutum to investigate their gene expansion, functional evolution, and potential association with key agronomic traits. A total of 51 GhFWLs were identified, and their gene structures, domain compositions, and phylogenetic relationships were comprehensively analyzed. Expression profiling under heat, cold, salt, and drought stress conditions revealed that most GhFWLs participate in abiotic stress response pathways. Haplotype-based association analysis revealed significant associations for 14 GhFWLs with fiber-related traits, suggesting their potential functions in regulating fiber development. Furthermore, silencing the family member GhMCA1 provided supporting evidence for its role in salt stress tolerance. Our findings provided insightful information about the FWL gene family in G. hirsutum and highlighted candidate genes for improving fiber-related traits and stress tolerance through molecular breeding approaches. Full article

Plasticized polyvinyl chloride (PVC-P) geomembranes (GMBs) are widely used in cold regions as impervious barriers, facing combined low-temperature and variable loading effects. However, design specifications rely on room-temperature tests at fixed rates, not reflecting actual conditions. This study systematically investigates the axial tensile properties of a 1.5 mm thick PVC-P GMB across eight temperatures (−40 °C to 20 °C) and five tensile rates (1–100 mm/min). A total of 211 uniaxial tensile tests were conducted using a low-temperature system with a servo-hydraulic machine and DIC extensometer. Nominal and true stress–strain curves were analyzed. Results show that fracture strength, fracture strain, and elastic modulus are highly sensitive to temperature and tensile rate, with a pronounced coupling effect between these two factors. Lower temperatures increase fracture strength and elastic modulus but reduce fracture strain, leading to brittle transition at −40 °C, especially at high rates. The fracture strength, fracture strain, and elastic modulus all increased with tensile rates at low tensile rates (1–20 mm/min). However, negligible difference in these parameters at high rates (20–100 mm/min) was observed. Elastic modulus follows a Boltzmann function with temperature, and fracture strain linearly correlates with temperature (R² > 0.93). The mechanical properties measured at room temperature overestimate the deformability at low-temperature and hence underestimate the brittle failure risk. Therefore, future cold-region testing should adopt tensile rates of 10 or 20 mm/min, and temperature-rate coupled constitutive models should be developed. These findings provide essential data and guidance for material selection, design, and standard revision for PVC-P GMBs in cold-region applications. Full article

Perennial wild relatives of annual crops possess traits associated with stress tolerance, efficient nutrient uptake, and increased soil carbon sequestration. They therefore represent valuable genetic resources for developing perennial crops that may reduce environmental and climate impacts and enhance food security. We evaluated 151 introgression lines (ILs) derived from crosses between barley and the perennial relative Hordeum bulbosum, each carrying unique H. bulbosum chromosome segments in a barley genetic background. Growth and regrowth after harvest were evaluated in two field trials, established by spring and fall planting in separate years in central Sweden, and under simulated two-year seasonal cycles in a climate chamber. In the climate chamber, 54% of the ILs exhibited strong regrowth, and two-thirds of these ILs showed reproductive growth across two seasonal cycles. In the field, most ILs showed regrowth after harvest but did not survive the cold winter. The parental genetic background had a strong effect from the barley parent on several growth and reproductive traits, but some ILs exhibited phenotypes not expected based on the barley parent. Of the divergent ILs with high regrowth, identified by PCA combining all studied traits, H. bulbosum introgressions at chromosome arms 2HL and 4HL were highly represented among ILs with cultivar Emir as a barley parent, and introgressions at 1HL among ILs with cultivar Morex as a parent. Perennial growth was observed only under mild conditions, indicating that perenniality of these ILs in a cold-temperate climate requires not only regrowth capacity but also adaptation to photoperiod, vernalization, and frost. ILs with high regrowth and reproductive growth may be used in crossing efforts with cold-tolerant germplasm to support the development of perennial barley. Full article

Poa pratensis var. anceps cv. Qinghai is a high-quality forage grass widely distributed across the Qinghai–Tibetan Plateau, exhibiting exceptional cold tolerance, a well-developed root system, and outstanding soil consolidation capacity, rendering it of great significance for ecological management in alpine regions. Jasmonic acid (JA), as an endogenous phytohormone, plays a pivotal role in regulating low-temperature adaptation and is hypothesized to be a core regulatory hormone underlying the cold tolerance of this cultivar. In this study, using seedlings of P. pratensis var. anceps cv. Qinghai (PQ) and the cold-sensitive cultivar P. pratensis “Barun” (PB) as a control, exogenous methyl jasmonate (MeJA, a JA analog) and the JA biosynthesis inhibitor DIECA were applied, and physiological and transcriptomic analyses were conducted to elucidate the mechanism by which JA regulates low-temperature adaptation. The results showed that MeJA significantly alleviated the growth inhibition induced by low temperature (4 °C) in both materials, with a more pronounced effect in PQ. Specifically, compared with the corresponding cold-only controls, MeJA treatment increased chlorophyll content by 5.55% in PQ and 22.40% in PB after 72 h of cold stress; soluble sugar content by 61.40% and 33.40%; soluble protein content by 47.16% and 36.50%; and proline content by 80.82% and 59.17%, respectively. MeJA also enhanced antioxidant enzyme activities, with SOD increasing by 49.28% and 15.61%, POD by 73.93% and 43.10%, CAT by 36.68% and 12.95%, and APX by 53.05% and 21.10% in PQ and PB, respectively. Additionally, non-enzymatic antioxidant contents (GSH and AsA) increased by 15.55% and 48.31% in PQ, versus 3.37% and 19.54% in PB. In contrast, DIECA suppressed these enhancements, with a more distinct inhibitory effect on PB, confirming that the JA pathway is a critical mechanism mediating low-temperature response in PQ, with significantly higher regulatory efficiency than that in the control. Transcriptome sequencing generated 1.787 million transcripts and identified 16,267 differentially expressed genes (10,054 up-regulated, 6213 down-regulated) in the MeJA-treated PQ group. GO and KEGG enrichment analyses revealed pathways associated with photomorphogenesis and carotenoid biosynthesis. Weighted gene co-expression network analysis (WGCNA) identified two core modules, and eight key genes including PpHY5 and PpCOR410 were screened, suggesting their coordinated roles in JA-mediated cold tolerance. These findings provide a comprehensive physiological and transcriptomic basis for understanding JA-enhanced cold tolerance in PQ seedlings. Full article

Stress-induced perturbations in rumen microbial community structure and function disrupt fermentation homeostasis, consequently impairing production performance and increasing health risks in ruminants. Quorum sensing (QS), a crucial mechanism governing microbial collective behavior and intercellular communication, is increasingly recognized for its role in ecological adaptation of rumen microbiota, yet direct evidence for its regulatory role in the rumen remains limited. This review summarizes the impacts of diverse stressors, including heat stress, cold stress, transport stress, and nutritional stress, on rumen fermentation characteristics and microbial community composition. Building upon this foundation, we discuss the alterations in QS signaling molecules and elucidate underlying microbial adaptation mechanisms from the perspectives of homeostasis disruption, signal transduction, and collective behavior regulation. Although QS-based nutritional and management strategies have shown preliminary potential for alleviating rumen stress, their effectiveness under practical production conditions requires further validation. This review provides a conceptual framework for understanding the potential role of QS in rumen microbial adaptation to environmental and nutritional stress, while highlighting current knowledge gaps and future research directions. Full article

NCED proteins play a critical role in drought, salt, and cold stress responses through ABA biosynthesis in plants. However, little information is currently available regarding the NCED gene family in Brassica species. Twenty-six putative BjNCED genes were identified in the genome of Brassica juncea, and found to be distributed on 15 chromosomes. Phylogenetic analysis suggested that these members could be classified into six subfamilies. The putative cis-elements were identified in the promoter regions of these BjNCED genes, and thought to be related to phytohormones, light, and abiotic stress responses. qRT-PCR analysis of five genes (BjNCED1, BjNCED4, BjNCED7, BjNCED16 and BjNCED18) revealed that, under salt stress, BjNCED16 and BjNCED18 showed transient induction, whereas exogenous ABA produced gene-specific and time-dependent expression patterns, including pronounced induction of BjNCED16 at 24 h. Low-temperature treatment strongly induced four of the five examined genes at 6 h. Analysis of the BjNCED genes in this study provides a valuable foundation for future investigations into the functional roles of the BjNCED family in response to growth, development and stress. Full article

Overwintering represents a critical bottleneck for farmed fish, during which low temperature and starvation stress frequently co-occur, yet their synergistic effects on fish health remain poorly understood. Here, we exposed Pelteobagrus vachelli, a cold-sensitive freshwater species, to four conditions for 10 days: control (25 °C, feeding), starvation alone (25 °C, starvation), cold alone (11 °C, feeding), and combined cold–starvation (11 °C, starvation). Hepatic histopathology, antioxidant and liver function indices, gut microbiota (16S rRNA sequencing), and untargeted metabolomics (LC–MS) were integrated to elucidate gut–liver axis mechanisms underlying synergistic injury. Combined stress synergistically aggravated liver injury, as evidenced by hepatocellular vacuolation and necrosis, elevated aspartate aminotransferase (AST), alanine transaminase (ALT), malondialdehyde (MDA), and suppressed total superoxide dismutase (T–SOD), glutathione (GSH), catalase (CAT), and total antioxidant capacity (T–AOC). Two-way ANOVA confirmed significant interactive effects between cold and starvation stress (p < 0.05). Multi-omics revealed that dual stress uniquely activated multiple cell death pathways (FoxO, autophagy, ferroptosis, apoptosis), with marked oxidative phosphorylation (OXPHOS) activation and glutathione depletion—a signature absent under single stressors. Gut microbiota restructuring showed beneficial commensals (Cetobacterium, Prevotella, Lactobacillus) depleted and opportunistic pathogens (Plesiomonas, Pseudomonas, Flavobacterium) enriched, with Plesiomonas identified as the dominant biomarker (LDA score = 4.82). Integrative networks identified these pathogenic genera as hubs linking metabolic dysregulation to liver damage. Collectively, combined cold–starvation induces synergistic liver injury via gut–liver axis disruption, driving metabolic reprogramming and oxidative damage. These findings provide candidate biomarkers for overwintering stress monitoring and inform management strategies to mitigate cold–starvation-induced hepatic injury in aquaculture. Full article

Reservoir stimulation is essential in enhanced geothermal systems and unconventional reservoirs where low permeability, inadequate fracture connectivity, or near-wellbore damage restricts commercial injection or production. This review evaluates hydraulic fracturing, thermal stimulation, plasma-pulse stimulation, and selected dynamic stimulation technologies. It compares their physical mechanisms, fracture-network development, reservoir applications, permeability enhancement, operational maturity, deployment challenges, and future perspectives. Hydraulic fracturing remains the most mature method for reservoir-scale fracture creation, fracture conductivity, and reservoir connectivity. In enhanced geothermal systems, however, performance depends on the heat-exchange area, distributed flow, thermal sweep, long-term energy recovery, and induced-seismicity control rather than permeability enhancement alone. Thermal stimulation is integral to geothermal reservoir development. Cold-fluid injection generates thermoelastic stress redistribution, enlarges the fracture aperture, activates natural fractures, promotes thermally assisted fracture propagation, and influences thermal breakthrough. Plasma-pulse stimulation, also termed pulsed-power plasma, electrohydraulic, or shock-wave stimulation, provides a low-water method for near-wellbore permeability enhancement, damage bypass, fracture reactivation, and restimulation. Its broader deployment remains constrained by the limited treatment radius, scale-up uncertainty, energy-transfer efficiency, tool durability, completion integrity, and insufficient field validation. Liquid CO₂ phase-transition, propellant, and explosive stimulation provide additional dynamic-loading options with distinct fracture responses, controllability, safety, and technology readiness. Stimulation technologies should therefore be selected according to the dominant reservoir limitation and evaluated using sustained injectivity or productivity, effective reservoir contact, distributed flow, delayed thermal breakthrough, treatment durability, wellbore integrity, and a controlled geomechanical response. Future progress requires hybrid stimulation, coupled thermal–hydraulic–mechanical–chemical (THMC) modeling, integrated monitoring, adaptive control, physics-informed artificial intelligence, digital twins, standardized field validation, and techno-economic and life-cycle assessments. Full article

Current thermal validation in food engineering commonly depends on rigid, temperature-only endpoints that generate thermal stress and the associated energy cost in the case of high-caliber matrices. To overcome this, this study provides a proof of concept for post-heating cooling inertia as the main kinetic driver of cumulative integrated lethality in mass-transfer-limited geometries (p value framework), followed by separate enzymatic and not microbial (or, e.g., oxidative) degradation following product heat transfer. Time-series and multi-spatial thermodynamic profiling was performed for the commercial validation runs of three pasteurization processes of large-diameter (90 mm) finely minced cooked chicken emulsions packed in polyamide casing. When active heating was stopped at a lower core limit of 71 °C, the thermal cooling inertia led total integrated lethality to increase by more than 216%, leading to an ultimate core p value of 76.87–89.56 min. It systematically exceeded the mandatory food safety limit (p ≥ 40 min) in all spatial coordinates in the forced convection chamber, confirming absolute thermal homogeneity. In a subsequent cold-chain stability challenge at around 70 days (0–4 °C), foodborne pathogens were not detected in the product, and saprophytic microflora was limited below critical spoilage bounds (total viable counts (TVC) and dominant lactic acid bacteria (LAB) progressively increased to 3.7 and 3.5 log10 CFU/g on day 70). The autoxidation pathways for primary and secondary lipids remained low (peroxide at 0.00 mmol/kg and malondialdehyde at less than 0.15 mg MDA/kg). On the other hand, a linear progression of free fatty acids (R² = 0.9673) determined that the single enzymatic lipid hydrolysis is responsible for the final biochemical limiting effect of the sensory lifespan. Shelf life evaluation via rigorous quantitative descriptive analysis showed that all organoleptic attributes remained well above market acceptance criteria until day 70. At the same time, this study supports a scalable, prediction-based thermodynamic paradigm that ensures biological safety and fundamentally optimizes industrial energy use. Full article

Diabetic painful neuropathy (DPN) is a common complication of diabetes. Despite ongoing efforts, the underlying biological mechanisms of DPN remain poorly understood. Hyperglycemia-mediated oxidative stress can affect cell barrier properties, triggering low-grade inflammation. Previous studies have reported increased histone deacetylase (HDAC) activity and altered expression of tight junction proteins in diabetes, which may link to nerve damage. This study examined the effects of the HDAC inhibitor FK228 on cell barrier-mediated changes in the spinal cord and dorsal root ganglia (DRG) of type 2 diabetic mice. Diabetic mice were treated with FK228 (1 mg/kg, twice a week for 3 weeks). Behavioral assessment of cold hypersensitivity was assessed at the end of the treatment regimen. In vitro studies were conducted using ND7/23 immortalized DRG cells. The expression of tight junction proteins (occludin, claudin-1, and zona occludens-1 or ZO-1) and a number of stress-related cellular markers, including HDAC2, growth-associated protein (GAP) 43, epidermal growth factor receptor (EGFR), and nuclear factor erythroid 2-related factor (Nrf2), were evaluated in DRG, spinal cord tissue, and cultured ND7/23 DRG cells following treatment. FK228 treatment demonstrated changes in cold pain sensitivity as well as the expression of tight junction proteins and stress related markers in both in vivo and in vitro models of diabetic neuropathy. These findings indicate that HDAC inhibition by FK228 may support the maintenance of tight junction protein expression and alter cellular stress responses in the spinal cord and DRG, suggesting a possible role of FK228 in mitigating DPN. Full article

Cold stress severely impairs grapevine (Vitis vinifera L.) growth, development, and productivity, necessitating the identification of elite cold-resistance genes for breeding tolerant cultivars. Chinese wild grape germplasm, particularly the endemic Vitis yeshanensis ‘Yanshan’ ecotype, represents a valuable reservoir of stress-resistance alleles with exceptional cold hardiness. In this study, we isolated an R2R3-MYB transcription factor gene VyMYB24 from ‘Yanshan’ grape and systematically characterized its function in low-temperature responses. VyMYB24 expression was rapidly and strongly induced by cold stress, with transcript levels peaking at 12 h after treatment. Heterologous overexpression of VyMYB24 in transgenic tobacco (Nicotiana benthamiana) induced pronounced architectural changes. Transgenic plants exhibited a dwarf and compact stature with enhanced lateral branching, thickened stems, and robust root systems. In addition, anatomical analysis revealed markedly increased xylem and phloem thickness. Under cold stress, transgenic lines outperformed wild-type plants, with reduced wilting, lower water loss, and improved survival and recovery rates. Physiologically, VyMYB24 activated the antioxidant defense system, elevated the activities of key reactive oxygen species (ROS)-scavenging enzymes, suppressed H₂O₂ and superoxide accumulation, and reduced malondialdehyde content and electrolyte leakage, thereby preserving cellular homeostasis. This study provides functional evidence for the positive regulatory role of VyMYB24 in cold tolerance via heterologous expression, underscores the genetic value of ‘Yanshan’ grape germplasm, and lays a preliminary foundation for improving crop stress resistance through the utilization of native wild germplasm genes. Full article

Fructans and fructooligosaccharides (FOSs) are structurally diverse fructose-based carbohydrates synthesized from sucrose through the coordinated action of fructosyltransferases. These compounds are widely distributed in plants and have attracted increasing attention due to their dual relevance in plant physiology and human nutrition. In plants, fructans function as dynamic carbon reserves and play key roles in tolerance to abiotic stresses such as drought, cold, and salinity. In parallel, their selective fermentability and prebiotic properties have positioned them as valuable functional ingredients in food and nutraceutical applications. This review provides an updated overview of fructan and FOS metabolism, focusing on their biosynthetic pathways, structural diversity, and physiological roles in plants. Particular attention is given to the occurrence and potential functions of fructans in fruits, a topic that remains comparatively underexplored. We also discuss recent advances in strategies aimed at enhancing fructan accumulation through breeding, metabolic engineering, and synthetic biology approaches. Finally, emerging opportunities for the biotechnological exploitation of fructan metabolism are highlighted, including crop biofortification, development of functional foods, and the improvement of plant resilience to environmental stress. Full article

Thermal variability associated with climate change is an increasing constraint for finfish aquaculture because water temperature directly affects feed intake, nutrient utilization, endocrine stress responses, oxidative balance, immunity, intestinal integrity and survival. The literature search was conducted without a lower publication-year restriction through 5 July 2026. Database and citation-linked searches identified 386 candidate records, of which 274 remained after duplicate removal; 71 full-text articles were assessed; and 28 peer-reviewed primary feeding studies with publicly available final versions of record were retained for qualitative evidence mapping. The eligible studies were published between 2012 and 2026, with 2012 representing the earliest eligible publication rather than a prespecified starting year. Functional aquafeeds have therefore emerged as promising nutritional tools for supporting physiological resilience under heat, cold and sub-optimal temperature conditions. This review developed a PRISMA-guided systematic evidence map of dietary functional-aquafeed strategies evaluated for thermal-stress tolerance, immune regulation, antioxidant defense and metabolic homeostasis in cultured finfish. The main synthesis emphasizes studies with direct dietary thermal-stress interventions and extractable mechanistic outcomes, including sodium butyrate, organic selenium, selenium nanoparticles with riboflavin, astaxanthin, Bacillus spp., propolis, bay laurel essential oil, Spirulina-based strategies and essential oils. Overall, the available evidence suggests that selected functional aquafeeds may support thermal-stress resilience through coordinated improvements in antioxidant capacity, reduced oxidative damage, modulation of cortisol/glucose and heat-shock responses, enhancement of innate and mucosal immunity, improved intestinal or tissue condition and stabilization of metabolic indicators. The most biologically coherent translational patterns were observed when dietary interventions improved multiple response domains simultaneously rather than a single biomarker. However, such cross-domain improvement was interpreted as mechanistic and translational support rather than as an independent indicator of evidence confidence, which depended primarily on independent replication, consistency of response direction and methodological quality. Nevertheless, the current evidence remains uneven across species, stress models, life stages and outcome domains, with limited data for marine carnivores, repeated temperature fluctuation, post-stress recovery, disease challenge and multiomics integration. Therefore, the findings should be interpreted as a structured evidence map rather than universal formulation guidance. Future studies should use standardized thermal-challenge protocols, dose–response designs, clear tank-level experimental-unit reporting and integrated biomarker panels to support practical development of climate-resilient functional aquafeeds. Full article

To elucidate the response characteristics of radial growth and water use strategies in coniferous forests of cold-arid regions to drought stress, this study focused on Larix sibirica in different forestry areas of the Altai Mountains. Using dendrochronology and stable isotope techniques, we calculated the basal area increment (BAI) and intrinsic water-use efficiency (iWUE), and combined these with the standardized precipitation–evapotranspiration index (SPEI) to identify drought events, to investigate tree growth and water-use efficiency responses to climate variability. The results showed that drought years were characterized by reduced radial growth and increased iWUE in Larix sibirica across both forest regions, and tree-ring-derived intercellular CO₂ concentration (Ci) increased with rising atmospheric CO₂ concentrations, whereas the Ci/Ca ratio remained relatively stable throughout the study period. Scenario analysis revealed that, prior to 1980, the long-term trend in iWUE was more consistent with the constant Ci scenario, suggesting relatively strong stomatal regulation. After 1980, iWUE trends became more closely aligned with the constant Ci/Ca scenario, indicating that trees maintained a relatively stable Ci/Ca ratio to balance carbon assimilation and water loss. With increasing drought severity, drought resistance declined in both forest regions; however, substantial spatial differences were observed in drought responses. Under moderate drought conditions, trees in the Haba-River forest area exhibited higher resistance, whereas trees in the Hanaslin forest area showed greater recovery capacity and ecological resilience. Winter temperature, growing-season temperature, late-season temperature, and water availability were identified as key climatic factors influencing variations in the radial growth and iWUE of Larix sibirica. Overall, under the combined influences of rising atmospheric CO₂ concentrations and increasing water limitations, Larix sibirica exhibited adaptive adjustments in carbon–water regulation; however, enhanced iWUE did not fully compensate for the negative effects of drought on radial growth. These findings provide valuable insights into the responses and adaptive strategies of cold-arid forest ecosystems under ongoing climate change and offer scientific support for the conservation and sustainable management of Larix sibiric forests. Full article