Search Results (196)

Diploid Vaccinium fuscatum is a wild blueberry species with a low chilling requirement, an evergreen growth habit, and soil adaptability to southeast US growing regions. Regardless of its potential to improve the abiotic and biotic resilience of cultivated blueberries, this species has rarely been used for blueberry breeding. One hurdle is the ploidy barrier between diploid V. fuscatum and tetraploid cultivated highbush blueberries. To overcome the ploidy barrier, vegetative shoots micro-propagated from one genotype of V. fuscatum, selected because it grew vigorously in vitro and two southern highbush cultivars, ‘Emerald’ and ‘Rebel,’ were treated with colchicine. While shoot regeneration was severely repressed in ‘Emerald’ and ‘Rebel,’ shoot production from the V. fuscatum clone was not compromised at either 500 µM or 5000 µM colchicine concentrations. Due to the high number of shoots produced in vitro via the V. fuscatum clone shoots of this clone that had an enlarged stem diameter in vitro were subjected to flow cytometer analysis to screen for induced polyploidy. Sixteen synthetic tetraploid V. fuscatum, one synthetic octoploid ‘Emerald,’ and three synthetic octoploid ‘Rebel’ were identified. Growth rates of the polyploid-induced mutants were reduced compared to their respective wildtype controls. The leaf width and length of synthetic tetraploid V. fuscatum and synthetic octoploid ‘Emerald’ was increased compared to the wildtypes, whereas the leaf width and length of synthetic octoploid ‘Rebel’ were reduced compared to the wildtype controls. Significant increases in stem thickness and stomata guard cell length were found in the polyploidy-induced mutant lines compared to the wildtypes. In the meantime, stomata density was reduced in the mutant lines. These morphological changes may improve drought tolerance and photosynthesis in these mutant lines. Synthetic tetraploid V. fuscatum can be used for interspecific hybridization with highbush blueberries to expand the genetic base of cultivated blueberries. Full article

In recent years, early olive fruit drop has been observed in the northern Mediterranean regions, causing significant economic losses, although the exact cause remains unknown. Recent studies have identified several possible causes; however, our understanding of how olive trees respond to these environmental stresses remains limited. This study includes an analysis of selected meteorological and flowering data for Olea europaea L. “Istrska belica” to evaluate the use of a chilling and forcing model for a better understanding of flowering time dynamics under a changing climate. The flowering process is influenced by high diurnal temperature ranges (DTRs) during the pre-flowering period, resulting in earlier flowering. Despite annual fluctuations due to various climatic factors, an increase in DTRs has been observed in recent decades, although the mechanisms by which olive trees respond to high DTRs remain unclear. The chilling requirements are still well met in the region (1500 ± 250 chilling units), although their total has declined over the years. According to the Chilling Hours Model, chilling units—referred to as chilling hours—represent the number of hours with temperatures between 0 and 7.2 °C, accumulated throughout the winter season. Growing degree hours (GDHs) are strongly correlated with the onset of flowering. These results suggest that global warming is already affecting the synchrony between olive tree phenology and environmental conditions in the northern Mediterranean and may be one of the reason for the green drop. Full article

Climate change has contributed to a decline in winter chilling accumulation, a critical requirement for budbreak in temperate fruit crops. Its consequence has been a reduction in fruit production. To compensate for insufficient chilling, hydrogen cyanamide (HC) is widely applied, though its effectiveness remains limited. This study investigated the effect of HC application on budbreak in low-chill kiwifruit under warm conditions by correlating phenological responses with changes in carbohydrate and nitrogen concentrations in bark tissues across bud positions. Phenological observations revealed the highest budbreak percentage and total flower buds at the apical position. HC significantly increased budbreak by 58.82% at the apical position and by 375% at the middle position, with corresponding increases in total flower buds by 148.78% and 1066.67%, respectively. Additionally, shoot lengths were uniform among bud positions in HC-treated canes, whereas non-treated canes showed shoot length heterogeneity. Moreover, HC treatment triggered an earlier and more pronounced reduction in soluble sugars (sucrose and hexoses) concentrations along the gradient from apical to basal bud positions, where the response was strongest at the apical position, which was strongly associated with enhanced budbreak percentages and total flower bud formation. While total nitrogen content was highest in the apical position, it was unaffected by HC application. These findings indicate that HC may promote budbreak by enhancing the mobilization and consumption of soluble sugars for bud growth, thereby improving budbreak performance, flower bud production, and uniform shoot development in low-chill kiwifruit under warm conditions. Full article

Fossil fuels, including coal, are a basis of energy systems in many countries worldwide. However, coal mining is associated with several difficulties, which include high temperatures within the coal mining area. It causes a need for cooling for safety reasons and also for the comfort of miners’ work. Typical cooling systems in mines are based on central systems, in which chilled water is generated in the compressor or absorption coolers on the ground and transported via pipelines to the air coolers in the areas of mining. The progressive mining operation causes a gradual increase in the distance between chilled water generators and air coolers, causing a decrease in the efficiency of the entire system and insufficient cooling capacity. As a result, it is necessary to increase the diameter of the chilled water pipelines and increase the cooling capacity of the chillers, which is associated with additional investment and technical problems. One solution to this problem may be the use of so-called ice slurry instead of chilled water in the existing mine cooling system. This article presents the cooling system, located in the mine LW Bogdanka S.A., based on ice slurry. The structure of the system and its key parameters are presented. The results show that switching from cooling water to ice slurry allowed the cooling capacity of the entire system to increase by 50% while maintaining the existing piping. This demonstrates the very high potential for the use of ice slurry, not only in mines, but wherever further increases in piping diameters to maintain the required cooling capacity are not possible or cost-effective. Full article

Pear (Pyrus pyrifolia) is an important deciduous fruit tree that requires a specific period of low-temperature accumulation to trigger spring flowering. The warmer winter caused by global warming has led to insufficient winter chilling, disrupting floral initiation and significantly reducing pear yields in Southern China. In this study, we integrated targeted phytohormone metabolomics, full-length transcriptomics, and proteomics to explore the regulatory mechanisms of dormancy in ‘Mixue’, a pear cultivar with an extremely low chilling requirement. Comparative analyses across the multi-omics datasets revealed 30 differentially abundant phytohormone metabolites (DPMs), 2597 differentially expressed proteins (DEPs), and 7722 differentially expressed genes (DEGs). Integrated proteomic and transcriptomic expression clustering analysis identified five members of the dormancy-associated MADS-box (DAM) gene family among dormancy-specific differentially expressed proteins (DEPs) and differentially expressed genes (DEGs). Phytohormone correlation analysis and cis-regulatory element analysis suggest that DAM genes may mediate dormancy progression by responding to abscisic acid (ABA), gibberellin (GA), and salicylic acid (SA). A dormancy-associated transcriptional regulatory network centered on DAM genes and phytohormone signaling revealed 35 transcription factors (TFs): 19 TFs appear to directly regulate the expression of DAM genes, 18 TFs are transcriptionally regulated by DAM genes, and two TFs exhibit bidirectional regulatory interactions with DAM. Within this regulatory network, we identified a novel pathway involving REVEILLE 6 (RVE6), DAM, and CONSTANS-LIKE 8 (COL8), which might play a critical role in regulating bud dormancy in the ‘Mixue’ low-chilling pear cultivar. Furthermore, lncRNAs ONT.19912.1 and ONT.20662.7 exhibit potential cis-regulatory interactions with DAM1/2/3. This study expands the DAM-mediated transcriptional regulatory network associated with bud dormancy, providing new insights into its molecular regulatory mechanisms in pear and establishing a theoretical framework for future investigations into bud dormancy control. Full article

This paper presents a method of evaluating energy and environmental factors before and after chilled water production system retrofitting at an industrial facility. A general algorithm was used for the analysis of chilled water system retrofitting at a pharmaceutics factory. Two retrofitting variants based on dual-stage absorption chillers supplied from an existing gas-fueled co-generation plant were identified. The proposed variants, i.e., tri-generation systems, were compared with the basic variant, which relied on electric compression water chillers. An evaluation of the variants was performed on the basis of two criteria: annual primary energy consumption and annual carbon dioxide emission. Variant 2, i.e., with a 1650 kW dual-stage absorption water chiller supplied from an existing gas fueled co-generation plant, was chosen as the optimal variant. It achieved a 370 MWh annual primary energy consumption reduction and a 1140 Mg annual carbon dioxide emission reduction. It was found that increasing the co-generation ratio for the CHP plant powering the pharmaceutical factory resulted in lower consumption of primary energy in variants in which the cooling energy supply system was retrofitted based on absorption water chillers. The threshold values of the co-generation ratio were e = 0.37 for Variant 1 and e = 0.34 for Variant 2. A literature survey revealed that there is limited interest in the application of such a solution in industrial plants. The performed analysis showed that the evaluated systems may nonetheless be an attractive option for pharmaceutics factories, leading to the reduction of primary energy consumption and carbon dioxide emissions, thereby making more electrical power available for core production. The lessons learned during our analysis could be easily transferred to other industrial facilities requiring chilled water production systems. Full article

Ornamental plants play a crucial role in the mitigation of urban heat islands. Recent decades have seen an increased frequency of abnormal climatic events like warm springs, but how these climatic events impact plant phenology in ornamental plants in urban areas is unclear. This study examines how climate fluctuations affect the flowering patterns (1963–2018) and thermal requirements of 28 woody ornamental species in Xi’an, a principal city in Central China. Years were classified as cold (<13.3 °C), normal (between 13.3 and 17.2 °C), or warm (>17.2 °C) based on March–May temperatures. The results show that the first flowering dates (FFDs) advanced by 10.63 days in warm years but were delayed by 6.14 days in cold years compared to normal years. Notably, thermal requirements (5 °C threshold) were 11.3% higher in warm years (343.05 vs. 308.09 °C days) and 9.4% lower in cold years (279.19 °C days), likely due to reduced winter chilling accumulation in warm conditions. While thermal time models accurately predicted FFDs in normal years (error: 0.33–1.37 days), they showed systematic biases in abnormal years—overestimating advancement by 1.56 days in warm years and delays by 3.42 days in cold years. These findings highlight that the current phenological models assuming fixed thermal thresholds may significantly mispredict flowering times under climate variability. Our results emphasize the need to incorporate dynamic thermal requirements and chilling effects when forecasting urban plant responses to climate change, particularly for extreme climate scenarios. Full article

Climate change challenges existing strawberry cultivars, requiring adaptation and the introduction of new varieties better suited to new climate conditions. This research evaluated the response over time of new advanced breeding selections (AN15,07,53, AN16,53,54 and AN12,44,60) derived from intraspecific crosses, proposed for the Mediterranean environment and organic greenhouse cultivation, by comparing plant yield and fruit quality at each harvest stage against five commercial strawberry cultivars (Dina, Arwen, Melissa, Marimbella, and Elide). Results showed that Dina, AN15,07,53, and AN16,53,54 had higher levels of soluble sugars, organic acids, and anthocyanins than the other cultivars evaluated. In addition, AN16,53,54 showed anticipated peak production and plant yield similar to that of commercial cultivars. Elide showed on average the highest total yield (632 g plant⁻¹), while Dina, AN15,07,53 and AN12,44,60 showed lower yields. The lowest and highest percentages of discarded fruits were recorded in Arwen (10%) and AN 12,44,60 (27.7%), respectively. Two genotypes, AN16,53,54 and AN15,07,53 are susceptible to further evaluation; AN16,53,54 showed appropriate features for organic systems management. An important feature related to the environmental conditions of the Mediterranean area is the precocity of production, combined with good quality properties. The genotype AN15,07,53 derived from two parents with high and low chilling requirements, would need to be evaluated for its performance under very different climatic conditions. Full article

The abiotic stresses associated with spring/fall freezes and extreme winter cold cause significant economic losses in blueberry production. These problems are exacerbated by climate change and increasingly erratic weather patterns. Developing freeze-tolerant blueberry cultivars with optimized cold hardiness, chilling requirement, and flowering and fruiting phenology holds promise for mitigating the risk of these weather-related damages. These weather-resilient cultivars will ensure the long-term productivity and sustainability of the blueberry industry. The focus of this review is to present the current understanding of the major components of genetic breeding for blueberry freeze tolerance, i.e., phenotyping, genotyping, genetic association analysis, and marker development. The advancement in gene regulation and corresponding proteomic changes upon cold acclimation, dormancy, de-acclamation, and flowering and fruiting aids in the understanding of the adaptive stress response in blueberries. A wide range of genetic diversity in freeze tolerance and phenological traits has been identified among cultivated and wild blueberry relatives. Significant efforts have been made to phenotype freeze tolerance, chilling requirement, and flower and fruit development in both field and controlled environmental conditions. Recent studies emphasize the need for high-throughput, image-based phenotyping of blueberry flower development to improve the precision and efficiency of selecting freeze-resilient genotypes. In addition, advancements in blueberry genomics and pangenome resources expanded the potential of variant calling and high-density linkage map construction. Genetic association studies have identified QTL regions linked to freeze tolerance in blueberries, providing valuable targets for selection. The implementation of these advanced genomic tools and high-throughput phenotyping methodology will accelerate the development of weather-resilient blueberry cultivars. Full article

The UK government has detailed guidelines for assessing and guiding LGBTQI+ asylum seekers through the asylum process in the UK. This is an explorative paper assessing whether post the enactment of the Nationalities and Boarders Act 2022 the media narrative around queer refugees has had any influence on UK government policy. It does this by laying out the baseline UK LGBTQ+ asylum seeker legislative and UK civil service guidance and analysing UK newspapers in a media data analysis of mainstream UK media websites to see if there is any media influence on legislation or guidance post-enactment of the NBA 2022. These findings indicate there was no discernible influence on UK government policy towards LGBTQ+ asylum seekers by mainstream UK news media (Mainstream media for this research is defined as national newspapers with large website traffic, such as The Guardian, The Times, and The Daily Mail. These sources were chosen due to their digital footprint and searchability, which enabled textual analysis of the content) post-enactment of the NBA 2022 on 28 April 2022. In addition, the doctrinal analysis shows that the UK government is both opaque about LGBTQ+ asylum statistics and the outcome of Stage 1 asylum decisions, making it difficult to judge what sources influence these decisions and data. This makes it difficult for the UK media to cover these stories with clarity unless a claimant waives their right to anonymity, or a leak happens. Given the available data, it is also apparent that there has been a year-on-year decrease in LGBTQ asylum appeals, suggesting that while the media narrative may not be influencing policy, the New Plan itself may be having a chilling influence on LGBTQ asylum claims. More research over a longer period of time is required to explore this. Full article

Precise detection of meat freshness levels is essential for food consumer safety and real-time quality monitoring. This study aims to achieve the high-accuracy freshness detection of chilled mutton freshness by integrating hyperspectral imaging with deep learning methods. Although hyperspectral data can effectively capture changes in mutton freshness, sparse raw spectra require optimal data processing strategies to minimize redundancy. Therefore, this study employs a multi-stage data processing approach to enhance the purity of feature spectra. Meanwhile, to address issues such as overlapping feature categories, imbalanced sample distributions, and insufficient intermediate features, we propose a Dual-Branch Hierarchical Spectral Feature-Aware Network (DBHSNet) for chilled mutton freshness detection. First, at the feature interaction stage, the PBCA module addresses the drawback that global and local branches in a conventional dual-branch framework tend to perceive spectral features independently. By enabling effective information exchange and bidirectional flow between the two branches, and injecting positional information into each spectral band, the model’s awareness of sequential spectral bands is enhanced. Second, at the feature fusion stage, the task-driven MSMHA module is introduced to address the dynamics of freshness variation and the accumulation of different metabolites. By leveraging multi-head attention and cross-scale fusion, the model more effectively captures both the overall spectral variation trends and fine-grained feature details. Third, at the classification output stage, dynamic loss weighting is set according to training epochs and relative losses to balance classification performance, effectively mitigating the impact of insufficiently discriminative intermediate features. The results demonstrate that the DBHSNet enables a more precise assessment of mutton freshness, achieving up to 7.59% higher accuracy than conventional methods under the same preprocessing conditions, while maintaining superior weighted metrics. Overall, this study offers a novel approach for mutton freshness detection and provides valuable support for freshness monitoring in cold-chain meat systems. Full article

The present study was conducted in Extremadura, a region in southwestern Spain with a significant area dedicated to olive cultivation. An analysis of the olive growing climatology of its territory was conducted using bioclimatic indices that affect the development of olive cultivation, focusing on water requirements, thermal requirements, and leaf carbohydrate synthesis. The study revealed that very dry conditions during the olive growing season are the main characteristic of the Mediterranean climate in the region. A principal component analysis was performed to analyze the main sources of variability, revealing two main components, determined by annual rainfall, annual water requirement, mean annual temperature, degree days above 14.4 °C accumulated during the olive growing season, and the number of days with optimal temperatures for leaf carbohydrate synthesis. Three homogeneous groups were determined by cluster analysis, one of which had cooler thermal conditions and no water requirements. The study found that an increase in the olive growing season or a shortening of the dormant period could result in a higher water input during the growing season and a lack of accumulation of chilling hours during the dormant period, causing crop maintenance problems in warmer locations. Climate change is expected to have significant impacts on this crop where climatic conditions are already very hot and dry. In the future, it is possible that the current olive-growing areas in Extremadura will move to other areas where the temperature is cooler. Full article

The MIKC-type MADS-box (MIKC) gene family is essential for controlling various plant developmental processes, including flowering time and dormancy transitions. Although the MIKC gene family has been widely studied across different plants, its characterization and functional study in herbaceous peony remain limited. In this study, 19 Paeonia lactiflora Pall. MIKC-type (PlMIKC) genes were identified from the transcriptome of a low-chilling requirement Paeonia lactiflora Pall. cultivar ‘Hang Baishao’. These MIKC genes were categorized into seven clades: six were classified as MIKC^C-type, including FUL/AP1, DAM, PI, AGL18, AGL12, AG, and SOC1, and one, AGL30, was classified as MIKC*-type. Notably, the FLC clade genes were absent in Paeonia lactiflora Pall. The PlMIKC genes were predominantly localized to the nucleus, and their sequences contained highly conserved MADS and K-domains. Phylogenetic analysis demonstrated that PlMIKC genes share a strong evolutionary affinity with the MIKC genes from grapevine (Vitis vinifera) and poplar (Populus trichocarpa). A low-temperature-induced bud dormancy transition (BDT) experiment revealed that PlMIKC genes, such as PlFUL and PlDAM, were highly expressed during dormancy maintenance, while PlSOC1, PlAGL12, and PlAGL30 were upregulated during BDT. Additionally, the transient overexpression of PlSOC1 in ‘Hang Baishao’ significantly accelerated BDT and promoted bud break, suggesting that SOC1, traditionally linked to flowering regulation, also plays a key role in dormancy transition. Since limited literature on the MIKC gene family is currently available in herbaceous peony, this study expands the knowledge of the MIKC genes in Paeonia lactiflora Pall. and offers valuable insights into the molecular regulation of bud dormancy in response to low temperatures. Full article

The flowering and fruiting of sweet cherry (Prunus avium L.) depend on precise synchronization with seasonal events. During harsh autumn and winter conditions, floral buds enter dormancy to protect and prepare for the productive season. Dormancy release occurs after exposure to genotype-specific chilling temperatures, an event in which epigenetic reprogramming triggers further metabolic and gene expression activation. Similarly, several Arabidopsis ecotypes require chilling (vernalization) to transition from vegetative to floral states. At vernalization’s end, the decrease in the repressor complex formed by SHORT VEGETATIVE PHASE (SVP) and FLOWERING LOCUS C (FLC) allows FLOWERING LOCUS T (FT) to induce flowering. However, this alone does not fully explain the process. MicroRNAs (miRNAs) play a crucial role in gene regulation during plant development and environmental interactions, and miR396’s role during flower development and vernalization has been described in some plant species, although not for sweet cherry dormancy. We used ‘Regina’, a high-chill sweet cherry variety, to identify candidate small RNA molecules throughout dormancy, resulting in the detection of miR396. The transcript expression levels of the putative miRNA target genes were evaluated through quantitative PCR analyses of dormant buds. Additionally, an artificial sweet cherry miR396 was used to transform Arabidopsis Edi-0, a vernalization-requiring ecotype. Ectopic expression of this artificial molecule partially mirrored the effect on target genes observed in dormant buds and, more importantly, led to vernalization-independent flowering. Artificial miR396 expression also resulted in decreased FLC and increased SVP and FT transcript levels. These results could pave the way for future studies on the involvement of miR396 in the regulation of dormancy and flowering, with potential applications in improving crop resilience and productivity. Full article

Dormancy is a vital adaptive strategy in temperate and boreal plants, particularly fruit trees, enabling them to withstand harsh winter conditions and ensure survival and synchronized growth resumption in spring. This review comprehensively examines dormancy, focusing on its physiological, environmental, and molecular mechanisms. Dormancy is characterized by two distinct phases: endodormancy, which is regulated by internal plant signals and requires cold temperatures for release, and ecodormancy, which is influenced by external environmental factors. These stages are intricately linked to seasonal temperature fluctuations and the plant’s ability to synchronize growth cycles, ensuring survival through harsh winters and optimal growth in warmer seasons. The review delves into the role of chilling requirements, temperature thresholds, and hormonal regulation in the dormancy process, highlighting how these factors influence critical growth events such as budbreak, flowering, and fruiting. Plant hormones, including abscisic acid, gibberellins, and cytokinins, regulate dormancy by modulating gene expression and growth activity. Additionally, we explore the historical development of dormancy research, from early observations of chilling requirements to the formulation of the chilling hours model. Considering ongoing climate change, the review examines how rising winter temperatures may disrupt dormancy cycles, potentially affecting the timing of flowering, fruiting, and overall crop productivity. This shift necessitates new strategies for managing dormancy, particularly in regions experiencing inconsistent or insufficient chilling. The review concludes by discussing practical approaches to enhance dormancy release and mitigate the impact of environmental stress on deciduous fruit tree growth, offering insights into improving agricultural practices amidst a changing climate. Full article