Search Results (177)

Aim: To prepare two different kinds of wound care creams containing plant extracts and examine their effectiveness in comparison with a placebo cream and a commercial wound care cream, Madecassol^®. Methods: The two cream formulations were developed using the same placebo cream (PC) as base cream. One formulation contained balsam of oriental sweet gum, or Levant storax, named as Levant Storax Cream (LSC); the other contained oil of Calendula, extract of St. John’s Wort, aescin (an extract of horse chestnut), and freeze-dried powder from Aloe vera (L.) Burm. f. leaf juice, designated as Complex Cream (CC). In the characterization of the creams, organoleptic properties, pH, viscosity, size distribution, and zeta potential of oil globules were measured. Furthermore, the stability of the creams was assessed under different environmental conditions. In vitro studies were performed by using an excisional wound model in rats to assess the potential of the creams for stimulating wound healing. The efficacy of LSC and CC was compared with a commercial reference cream, Madecassol^® (M), and the placebo control. The study was also designed with a negative control group of rats that were not treated but handled the same way as the other treatment groups. The wound contraction rate, total skin thickness recovery, and results of histopathological parameter examinations were used to compare the effectiveness of the treatments. Results: The stability of formulated creams confirmed that they were stable for the duration of the study. In vivo studies showed that rats treated with LSC achieved the highest wound healing rates when compared with the other groups. A better response was recorded for the CC-treated population when compared to both control and placebo groups, but there was no significant difference seen in healing score between CC and M groups. Full article

Chestnut (Castanea sativa Mill.) is an edible nut recognized for its nutritional attributes, particularly its elevated levels of carbohydrates (starch) and proteins. Chestnuts are popular for their health-promoting properties and hold significant environmental and economic importance in Europe. During this study, after the characterization of the fruit, attention was directed toward the valorization of chestnut shells, a predominant by-product of industrial chestnut processing that is typically discarded. Valuable bioactive compounds were extracted from the shells using Pressurized Liquid Extraction (PLE), a green, efficient, scalable method. Response surface methodology (RSM) was utilized to determine optimal extraction conditions, identified as 40% v/v ethanol as the solvent at a temperature of 160 °C for 25 min under a constant pressure of 1700 psi. High total polyphenol content (113.68 ± 7.84 mg GAE/g dry weight) and notable antioxidant activity—determined by FRAP (1320.28 ± 34.33 μmol AAE/g dw) and DPPH (708.65 ± 24.8 μmol AAE/g dw) assays—were recorded in the optimized extracts. Ultrahigh-performance liquid chromatography coupled with a hybrid trap ion mobility-quadrupole time-of-flight mass spectrometer (UHPLC-TIMS-QTOF-MS) was applied to further characterize the compound profile, enabling the identification of phenolic and antioxidant compounds. These findings highlight the possibility of using chestnut shell residues as a long-term resource to make valuable products for the food, medicine, cosmetics, and animal feed industries. This study contributes to the advancement of waste valorization strategies and circular bioeconomy approaches. Full article

Background/Objectives: Natural products such as honey and propolis have been widely studied for their antimicrobial properties. Combining these substances has shown synergistic effects against foodborne pathogens and has also demonstrated promising results in previous applications on fermented meat products. This study evaluated the antibacterial potential of Spanish thyme (Thymus spp.) and chestnut (Castanea sativa) honeys, enriched with 10% ethanolic extract of propolis, against two major foodborne pathogens: Listeria monocytogenes and Clostridium perfringens. Methods: Antibacterial activity was assessed using broth microdilution assays and colony-forming unit (CFU) counts. The phenolic composition of the most active samples was characterized by LC-MS-Q/TOF and UPLC-PDA to identify and quantify the bioactive compounds. Results: All samples exhibited differential responses depending on the pathogen, with C. perfringens being the most susceptible. Propolis addition significantly enhanced the bactericidal response of honey against L. monocytogenes and C. perfringens (p < 0.05). This effect correlated with higher levels of antimicrobial phenolic compounds, particularly cinnamic acid derivatives, pinobanksin-3-O-hexanoside, sakuranetin, quercetin, and quercetin-3,7-dimethyl ether. Conclusions: These findings support the synergistic antibacterial potential of honey-propolis combinations, highlighting their application as natural preservatives for reducing the risk of foodborne diseases, as well as bioactive ingredients in nutraceutical formulations with antibacterial properties and additional health benefits. Full article

Background/Objectives: Chestnut inner shells, traditionally used in Korean and Chinese herbal medicine, contain antioxidant and anti-inflammatory compounds that contribute to complementary medicine. This study aimed to explore the therapeutic effects of chestnut inner-shell extract (CIE) on skeletal muscle injury and atrophy using both in vivo and in vitro models. Methods: We used three experimental models representing distinct pathological mechanisms: (1) barium chloride (BaCl₂)-induced muscle injury to model acute myofiber damage, (2) sciatic nerve transection to model chronic neurogenic muscle atrophy, and (3) H₂O₂-treated C2C12 myoblasts to model oxidative-stress-related myogenic impairment. Histological analyses (e.g., hematoxylin and eosin staining and cross-sectional area measurement) and molecular analyses were performed to evaluate the effects of CIE on muscle structure, apoptosis, and oxidative stress. Results: In the BaCl₂ injury model, CIE treatment significantly restored the muscle fiber structure, with muscle protein levels returning to near-normal levels. In the denervation-induced muscle atrophy model, CIE treatment led to a dose-dependent decrease in apoptosis-related factors (especially cleaved caspase-3) and mitigated the Akt/mTOR signaling pathway. In the in vitro oxidative stress model, CIE suppressed the expression of NRF2 and HO-1, which are key oxidative stress response regulators. Conclusions: These findings suggest that CIE may offer therapeutic potential for mitigating skeletal muscle damage, atrophy, and oxidative stress. Full article

Chestnut blight, caused by Cryphonectria parasitica (Murrill) M.E. Bar, is a destructive fungal disease threatening chestnut cultivation and production. In response to the limitations of chemical control, biological control using antagonistic microbes has gained increasing attention. A rhizosphere-derived bacterium, strain D39, was isolated from healthy chestnut trees and identified as Bacillus amyloliquefaciens based on morphological characteristics and the phylogenetic analysis of 16S rRNA and gyrA genes. The antifungal activity of strain D39 against C. parasitica was evaluated using dual-culture and double-layer Oxford cup assays. The strain exhibited broad-spectrum and stable antagonistic effects and harbored five key genes associated with antimicrobial compound biosynthesis (srfAA, ituC, fenD, bmyB, and bacA), as confirmed by PCR. A 145 kDa extracellular protein with strong antifungal activity was extracted and purified by ammonium sulfate precipitation, DEAE ion-exchange chromatography, and Sephadex G-75 gel filtration. LC-MS analysis identified the protein as a serine peptidase belonging to the S8 family, and its structure was predicted using multiple bioinformatic tools. In pot experiments, treatment with the strain D39 significantly reduced disease severity, achieving control efficiencies of 66.07% and 70.89% at 10 and 20 days post-inoculation, respectively. These results demonstrate that B. amyloliquefaciens D39 has strong potential as a biocontrol agent against chestnut blight, offering an effective and environmentally friendly alternative for disease management. Full article

Few studies have investigated the influence on physicochemical and phytochemical compositions during storage in the bottle of wine spirits (WSs) aged using alternative ageing technology (AAT) compared to traditional ageing technology (TAT). The aim of this study was to evaluate the effect of the bottle storage over one and four years on the evolution of chromatic characteristics (CIELab method) and physicochemical characteristics (alcoholic strength, acidity, and total dry extract), total phenolic index (TPI), low molecular weight compound contents (HPLC-DAD technique), in vitro antioxidant activities (DPPH, FRAP, and ABTS assays), and phenolic characterisation (HPLC-DAD-ESI-MS/MS technique) of WSs aged with chestnut wood using TAT (barrels, B) and AAT (micro-oxygenation levels (MOX): O15, O30, and O60; and control (N)). The results showed that after four years of storage in the bottle, the O60 modality resulted in smaller changes in physicochemical characteristics, higher preservation of phenolic content, and greater evolution of chromatic characteristics, ensuring its overall quality compared to other modalities. Antioxidant activity decreased similarly in both technologies, such as phenolic acid content, in particular, gallic acid content. According to the findings of this study, alternative ageing technology might be the best alternative for wine spirit quality and ageing process sustainability. Full article

Mead is described as a traditional alcoholic drink produced by fermentation from a solution of honey and water. It has been produced as a refreshing drink. However, in the past, it was more expensive than wine, which led to a decrease in demand. Due to the simple method of production, the mead industry is growing again. The quality and physicochemical properties of mead depend on the type of honey used. The goal of this study is to produce mead from two kinds of honey of different floral origins, chestnut and sunflower, in order to determine the differences using sensory and physicochemical analyses. The fermentation process was monitored until the extract values were consecutively the same. The results obtained in this research indicate that chestnut honey mead took a longer time to ferment, 2 months, while sunflower honey mead took 1.5 months to ferment. The alcohol content in chestnut honey mead was 7.2% v/v, and sunflower honey mead contained 8.6% v/v. Sensory-wise, the chestnut mead was more acceptable to consumers due to a more pronounced color and thus received a one-point higher score (15) than sunflower honey mead (14). Full article

Industrial residues are sources of functional biopolymers with interesting properties for textile applications. This study aims to evaluate the impact of enzymatic pre-treatment on oil yield and phenolic compounds’ content in an aqueous extraction process, as well as the functional properties incorporated into textiles. This research investigated the influence of residue granulometry, biomass percentage, and the application of enzymatic pre-treatment with different enzymes (cellulase, pectinase, xylanase) individually or in combination. Chestnut hedgehog (CH), tobacco plant stems (TPSs), vine shoot trimmings (VSTs), and beer spent grain (BSG) were explored. For textile functionalization, the extracted oils were incorporated into a bio-based formulation and applied on cotton fabric through pad-dry-cure. For CH, the pre-treatment with cellulase and xylanase achieved an oil yield of 149 and 148 mg oil/mL extract, respectively. With the combination of both enzymes, the richest oil in phenolic compounds was extracted: 1967.73 ± 16.86 mg GAE/g biomass. CH and TPS oils presented an antioxidant activity above 60%, and the functionalized textiles also showed the highest antioxidant potential and a UPF of 30. The textiles presented water repellence and washing fastness. This study demonstrates a sustainable oil extraction method and its potential application in the development of functional textiles. Full article

This study evaluated the use of brewer’s spent yeast (BSY) as an adsorbent for tannins from a chestnut shell extract (CS tannin extract). This extract was derived from an alkaline treatment (5% NaOH (v/v)) to recover cellulosic material from chestnut shells and needed valorization. Various BSY treatments, including lyophilization, immobilization in calcium alginate beads, and alkaline and acid treatments, were tested to identify which had the best tannin adsorption capacity. The results highlight BSY’s potential as a system to valorize tannins from this treatment solution. Full article

The irradiation by gamma-rays is a widely used technique for disinfection in the pharmaceutical and cosmetic industries. In view of growing concerns by consumers about this technique, further investigation of the effects of radiation is required. In this work electron paramagnetic resonance (EPR) spectroscopy was applied to study the free radicals in irradiated horse chestnut (Aesculus hippocastanum L.) seeds and to evaluate the free radical scavenging activity (FRSA) using the stable DPPH radical. In order to evaluate the antiradical potential, a spectrophotometric study was also used. The identification and quantification of some individual polyphenol compounds before and after irradiation by 1, 5, and 10 kGy gamma rays of peeled and shell seeds were obtained by high performance liquid chromatography (HPLC). The EPR spectrum recorded on irradiated horse chestnut is a typical signal for irradiated cellulose-contained substances. The results show that the signal is stable, and it can be found in the samples irradiated with a dose of 1 kGy, 45 days after treatment, whereas for samples irradiated by 5 and 10 kGy, it is even found 250 days later. The study showed that free radical scavenging activity increases in shell seeds, while it decreases in peeled seed extracts after irradiation depending on the dosage, which corresponds to the total phenolic content. Shell seed extracts have significantly stronger antiradical activity than that of peeled seeds. Regarding the HPLC analysis, some polyphenolics were degraded and others were formed as a result of irradiation. The irradiation by 5 kGy dosage has a most significant positive effect on the antioxidant potential of shell chestnut seeds. Full article

The aim of our study was to compare the composition of polyphenolic compounds between the Croatian acacia (Robinia pseudoacacia L.) and chestnut (Castanea sativa Mill.) honey from several aspects: production season, hive position (on the edge and in the middle of a series of hives), part of the hive (small or normal extension), and honey extraction method (centrifuging or draining honey combs). Additionally, in acacia honey, we also monitored the influence of different storage temperatures (room temperature (RT) and 4 °C) on the content of phenolic compounds. To separate, identify and quantify individual flavonoids and phenolic acids from the honey, we used the HPLC method. The total polyphenols and antioxidant activity of the samples, their antimicrobial activity and their elemental content were also measured. The significant influence of the season, hive position, and extraction method on the total identified phenolic compounds, phenolic acids, flavonoids, total phenols and antioxidant activity was detected in almost all the acacia and chestnut honey samples. Chestnut honey from 2013 had more total phenolics (TPs) and antioxidant capacity (FRAP) than chestnut from 2014 and 2015. Honey collected from smaller extensions of hives had significantly higher TPs and FRAP compared to normal hive extensions. Centrifugation reduced the TPs and FRAP in most cases, but not always uniformly. Storage at RT led to the predominance of gallic, p-coumaric and benzoic acid in acacia honey, while storage at 4 °C maintained p-coumaric acid as the dominant phenolic acid. Flavonoids, particularly pinobanksin in acacia honey and hesperetin/pinobanksin in chestnut honey, were less affected by the storage conditions compared to phenolic acids. The non-centrifuged chestnut sample from 2015 showed the lowest MIC values against the most tested pathogenic bacteria. All the honey samples showed an extremely low concentration of heavy metals and relatively high concentrations of potassium and calcium. Full article

Fermented soybean meal (FSM) can improve protein utilization efficiency but is easily degraded in the rumen; therefore, it cannot provide sufficient metabolizable protein (MP) for dairy cows. Here, the rumen degradation characteristics of FSM combined with tannin extracts from quebracho or chestnut trees or heat-treated were investigated regarding in vitro gas production and in situ in the rumens of three fistulated cows. The results of the rumen in situ incubation showed that the dry matter (DM) degradation rate decreased in the three rumen treatments. Compared with those of the FSM, the effective degradation rate of the FSM with chestnut tannin was significantly reduced and the rumen undegradable protein was significantly increased. The MP% (DM%) and MP (DM% × DM recovery) indicators in the FSM with chestnut or quebracho tannins increased, but the difference was not significant, whereas those of the FSM decreased following heat treatment. The results concerning in vitro gas production showed that tannin could reduce the rate of biogas production of the slowly degraded part. In conclusion, chestnut tannin is more suitable for protecting FSM from rumen degradation than heat treatment. This study provides a feasible treatment solution for applying FSM to dairy cows, which improves the soybean meal protein utilization efficiency and reduces the amount of soybean meal used. Full article

This study presents findings on the metal and metalloid composition of red horse-chestnut (Aesculus × carnea, AXC) seeds, determined by the ICP OES technique. Samples were collected from five AXC plants located in Modena (Italy) over four consecutive years (2016–2019). The seeds underwent proximate analysis, which included measurements of moisture content, proteins, lipids, carbohydrates, ash, and elemental composition. The analysis revealed consistent values for these parameters throughout the study period. The metal content of the AXC seeds can be categorized into two groups: (i) major components, with concentrations ranging from 1 to <1500 mg/100 g dry basis (d.b.), where K was identified as the most abundant element, and (ii) minor constituents, with concentrations between 1 and <1000 μg/100 g d.b., with Li, Mo, and Ti at the lower concentration limit. Comparative analyses were performed using literature data on AHP and AHH seeds, which, like AXC, belong to the Sapindaceae family and were collected from the same area and period. A hydroalcoholic extract of AXC seeds was prepared annually and characterized, with results compared to a commercial product (AXC_herb). AXC extracts had approximately 30% higher analyte concentrations than AXC_herb, while AXC seeds showed 20–30% higher metal and metalloid levels than AHP and AHH. Full article

Background/Objectives: Irritable Bowel Syndrome (IBS) is a disorder of the gut-brain axis for which the gastroenterologist is most often consulted. Gastrointestinal symptoms and decreased quality of life lead to a considerable burden of disease. The exact causes of IBS are not well understood, and no standard therapy has been established. The primary outcome of the study focused on the improvements of the IBS symptoms assessed through the validated questionnaire IBS-Severity Scoring System (IBS-SSS). Similarly, secondary outcomes geared towards the improvement of the quality of life (IBS-Quality of Life (IBS-QoL) and the Gastrointestinal Quality of Life Index (GIQLI)) and specific IBS symptoms (bloating, abdominal distension, feeling of heaviness, abdominal pain, and flatulence), were assessed through self-administered questionnaires. Intestinal habits (consistency and frequency of depositions) through subject stratification into diarrhoea (IBS-D), constipation (IBS-C), mixed type (IBS-M), as well as the treatment tolerability were also evaluated. Methods: A randomised, placebo-controlled, double-blind, clinical trial was conducted on 156 enrolled IBS patients (79 female and 77 male), aged 18–70 years, randomised (1:1 allocation ratio) to receive either two capsules per day of the food supplement (containing 480 mg of a complex of tannin extracts) or a placebo for 56 days. Linear random intercept mixed models (LMM) were used to analyse all experimental variables Results: Supplementation resulted in a significant improvement (p < 0.05) in the primary outcome IBSS score, with respect to the placebo group, changing the IBS condition going from mild (242.3 ± 89.8) to moderate (148.1 ± 60.6). Similarly, all indicators concerning quality of life, and specific intestinal symptoms resulted in a significant improvement (p < 0.05). Furthermore, the tannin-based treatment showed the ability to modulate the response to different symptomatology such as diarrhoea and constipation, without side effects being reported. Conclusions: The use of a supplement based on chestnut and quebracho tannins presents great application potential in the management of IBS-related disorders, with the peculiarity of resolving opposite symptoms, such as diarrhoea and constipation, indiscriminately. Full article

Rizhao green tea (RZT), a renowned green tea, is cultivated in China’s northernmost tea region. Its unique environment endows it with a strong chestnut- and seaweed-like aroma. This study sought to explore the volatile profiles of RZT and pinpoint its key odorants by employing stir bar sorptive extraction (SBSE) coupled with gas chromatography–mass spectrometry (GC-MS), determining the odor activity value (OAV), and performing gas chromatography–olfactometry (GC-O). A total of 112 volatiles were identified, and the major volatile compounds were esters (2035.25 μg/kg), alcohols (1799.02 μg/kg), alkanes (991.88 μg/kg), and ketones (691.96 μg/kg), comprising 74.91% of the total. A molecular aroma wheel was preliminarily established based on these key odorants. These insights might contribute to the scientific elucidation of the flavor chemical basis of RZT. Full article