Search Results (416)

Reproductive performance in livestock and poultry is a core determinant of economic efficiency in the animal industry. While traditional research has primarily focused on genetics, endocrinology, and immune regulation, emerging microbiome studies reveal that commensal microbiota within the gut and reproductive tracts play an underestimated yet pivotal role in host reproductive health. This review systematically synthesizes recent advances regarding the relationship between the microbiome and reproductive functions in major livestock species (cattle, pigs, sheep, and chickens). We first delineate the theoretical basis and mechanisms of the “gut-reproductive axis,” highlighting cross-system communication mediated by microbial metabolites, including short-chain fatty acids (SCFAs), indoles, and bile acids. Subsequently, we provide an in-depth comparative analysis of the microecological features of both female (vagina/uterus) and male (semen/epididymis) reproductive systems, examining their impacts on fertility, sperm quality, and pregnancy outcomes. Furthermore, we explore the molecular and systemic mechanisms governing microbial regulation of reproduction, encompassing the modulation of the hypothalamic-pituitary-gonadal (HPG) axis, the balance of local mucosal immunity and inflammation, and epigenetic regulation. Finally, we address current challenges—such as causal validation and the scarcity of multi-species databases—and propose future directions, including spatial multi-omics, AI-integrated analysis, and microbial intervention strategies. Ultimately, this review aims to offer a theoretical foundation and translational insights for elucidating reproductive regulatory networks and developing microbiome-driven precision strategies to enhance reproductive performance. Full article

Foot-and-mouth disease virus (FMDV) infection elicits sustained, high-level interleukin-10 (IL-10) secretion in cattle and pigs, which correlates with lymphopenia and immunosuppression. We previously showed that macrophages are the principal source of IL-10 during FMDV infection in mice, but the viral trigger and host pathways remained unknown. In the present study, we examined whether the FMDV structural protein VP3 regulates IL-10 expression. To this end, a eukaryotic VP3 expression vector was transfected into porcine alveolar macrophages (3D4/21 cells), and IL-10 expression together with related signaling pathways was interrogated by qRT-PCR, ELISA, Western blot, co-immunoprecipitation (Co-IP), confocal microscopy, and luciferase reporter assays. The results showed that VP3 significantly increased IL-10 mRNA and protein levels (p < 0.001) in a time-dependent manner. Mechanistically, VP3 promoted phosphorylation of PI3K, AKT, and mTOR; this effect was abolished by the PI3K inhibitor LY294002, which also abrogated VP3-induced IL-10 secretion (p < 0.05). Furthermore, VP3 upregulated mRNA expression of STAT3, ATF1, and CREB (p < 0.05) and enhanced IL-10 promoter activity. The STAT3 inhibitor Stattic reduced IL-10 secretion by 22% (p < 0.05). Co-IP and confocal microscopy confirmed direct binding of VP3 to PI3K in the cytoplasm. In conclusion, FMDV VP3 induces IL-10 overexpression by directly activating the PI3K/AKT-mTOR signaling pathway, thereby elucidating a key mechanism of FMDV-induced immunosuppression. Full article

The growing demand for robust food authentication methods has driven the establishment of fast, sensitive, and field-based detection systems for identifying meat species. This study presents a colorimetric-based PSR approach for identifying yak (Bos grunniens) meat within fresh, thermally processed, and blended meat samples. Targeting the mitochondrial D-loop locus, the assay incorporates a simple alkaline lysis (AL) procedure for efficient DNA extraction, eliminating the requirement for specialized instrumentation. The PSR assay demonstrated high specificity, showing no evidence of cross-reactivity with closely associated food animals such as buffalo, cattle, goat, sheep, mithun, and pig. Sensitivity assessment revealed the assay’s capability to detect 1 pg of yak DNA, with reliable performance in samples exposed to thermal conditions up to 121 °C. Additionally, the technique detected yak meat down to a concentration of 0.1% in binary beef mixtures. This method provides a significant improvement in sensitivity over end-point PCR and is particularly well-suited for field applications due to its practical simplicity, affordability, as well as no reliance on sophisticated instrument. This is, to the best of our understanding, the first reported PSR-based approach developed for the identification of yak meat, offering a robust tool for food origin verification, regulatory enforcement, and product integrity monitoring. Full article

Dog overpopulation poses serious challenges to public health, animal welfare, and environmental sustainability. While surgical castration remains the most commonly used method for controlling reproduction in dogs, it carries risks and limitations, including surgical complications and long-term behavioral or physiological side effects. This review examines the potential of immunocastration—vaccination targeting gonadotropin-releasing hormone (GnRH)—as an alternative approach. Immunocastration has been shown to suppress reproductive hormones, reduce gonadal function, and decrease behaviors such as roaming, aggression, and sexual mounting in dogs. The review also includes evidence from other species (e.g., pigs, cattle, and horses) to contextualize effectiveness and welfare implications. While behavioral effects are more variable and less frequently studied than physiological outcomes, recent findings suggest immunocastration may have fewer negative emotional side effects (e.g., anxiety and stress-related behaviors) than surgical sterilization. The review highlights the mechanisms, applications, and behavioral outcomes of immunocastration, including its reversibility, cost-effectiveness, and suitability for mass implementation. Overall, immunocastration offers a promising addition to dog population management strategies, particularly in regions with limited surgical infrastructure. Future research should focus on standardizing vaccine protocols and assessing long-term behavioral and welfare outcomes in diverse dog populations. Full article

This case study is an overview of architectural design solutions implemented in the construction of farming facilities and the technological processes necessary to support a sustainable farm that runs with nearly zero waste in a closed-loop system that functions with full energy independence. The research will thoroughly investigate the specific location and configuration of the farm units in the target area—providing an extensive description of all necessary building typologies and infrastructures. The text will provide a summary of the agricultural solutions implemented at the farm, which is located in the region of Vojvodina in the Republic of Serbia. This region consists mainly of fertile agricultural land and could be a template for further designs and innovations in sustainable farming. This case study concerns the design of a resilient and self-reliant farm complex that consists of multiple animal species (broilers, pigs, and cattle), including a biogas station. The study is meant to show that adjustments made in architectural design, variations in building typology, and smart urban planning can contribute significantly to the improvement of sustainability in agricultural practices. This case study demonstrates that investments in sustainable solutions not only benefit the environment but can also deliver significant economic returns for investors—thereby further stimulating growth and development in the field of sustainable agriculture. Full article

The remaining problems in pig farming may no longer be solved with traditional methods. The search for genetic variants associated with desired characteristics and involvement of animals with superior genetics in breeding programs is rarely effective for polygenic traits and pleiotropic genes. The lack of diversity in the germplasm also limits the use of breeding, but some beneficial mutations that did not occur naturally can be introduced manually via genome editing methods. Mutations discovered in other species, such as cattle, can be reproduced in pigs. Traits that were previously pursued for centuries might be achieved by genome editing in a few years. Enormous progress has been made in producing pigs resistant to viruses and in increasing meat productivity and quality. But there are still pressing problems such as lameness and damaging behaviors that probably cannot be solved without genome editing techniques. Their wider application is complicated by the requirement for large amounts of biomaterial, surgical manipulations and cell culture, as well as by the shift towards biomedical research. This review concentrates on the main achievements and challenges in pig agricultural genetics that can be addressed by genome editing. Full article

Heavy metals in livestock and poultry manure cause significant contamination; however, there is currently a lack of scenario analysis research on soil pollution risks under the influence of manure application. This study integrated multiple methods, including multi-source data fusion, heavy metal emission accounting, and ecological risk assessment, to investigate regional soil heavy metal pollution risks under baseline and improved scenarios of manure application, using Hunan Province, China, as a case study. The results indicate that pig manure (49.5%) and cattle manure (47.6%) are the primary sources of heavy metal emissions from livestock and poultry manure. The heavy metal loads on cropland (g/ha) were as follows: Cd (0.51), Hg (0.027), As (0.87), Pb (4.69), Cr (5.38), Cu (93.10), Zn (131.05), and Ni (5.07). Among the eight heavy metals, Cd poses the most prominent soil pollution risk. Under the baseline scenario (100% manure application), the study area exhibited an overall moderate ecological hazard level after 37 years of continuous application, with 71.93% of the cropland classified as Risk Level II and 7.04% as Risk Level III. After 184 years, a strong ecological hazard level was reached, with 54.93% of the cropland classified as Risk Level III and 19.64% as Risk Level IV. Under improved scenarios (75%, 50%, and 25% manure application), the overall moderate ecological hazard level was reached after 49, 74, and 147 years of continuous application, respectively. This study provides a theoretical and methodological basis for regional soil heavy metal pollution control and source analysis. Full article

Anaerobic digestion (AD) provides significant environmental benefits by converting livestock manures, such as cattle manure (CM) and pig manure (PM), into biogas and nutrient-rich digestate, supporting circular economy principles. However, challenges arise when feedstock overload disrupts microbial balance, leading to reduced methane (CH₄) yields and process instability. This study examined the performance of AD using CM and PM with gradually increasing organic loading rates (OLR). At steady state, CH₄ yields were 120.32 mL-CH₄/g VS for CM and 229 mL-CH₄/g VS for PM. The lower yield for CM is attributed to its high cellulose and hemicellulose content, which exceeds 50% and is difficult to degrade. In contrast, PM showed more efficient carbohydrate degradation, resulting in higher CH₄ production. Key methanogens, including Methanocorpusculum, Methanosaeta, Methanosarcina, Methanobacterium, and Methanospirillum, were present in both reactors. Metagenomic analysis revealed that pathways for degrading cellulose and hemicellulose were poorly represented in CM, while PM exhibited enhanced total volatile fatty acid metabolism. This study offers valuable insights into the metabolic pathways associated with CM and PM in anaerobic digestion. Full article

The conversion of livestock manure and peat into value-added fertilizers provides an environmentally sustainable approach to nutrient recycling and waste management. In this study, organic fertilizers were formulated from poultry, pig, and cattle manure mixed with peat and wood ash, with or without inoculation of the phosphate-solubilizing bacterium Priestia megaterium. Their efficiency was evaluated through plant growth and soil microbiological experiments involving conifer seedlings, herbaceous crops, and ornamental plants. Germination and growth trials with Norway spruce (Picea abies) and Scots pine (Pinus sylvestris) revealed clear species-specific responses: spruce seedlings performed best in substrates containing poultry or cattle manure, while pine showed enhanced growth with pig manure combined with bacterial inoculant. In pansies (Viola × wittrockiana), growth responses varied by cultivar; cattle manure enriched with bacteria increased leaf projection area, whereas poultry manure markedly suppressed growth. For cucumbers, basil, barley, radish, and garden beans, yields were lower than with mineral fertilizers, yet bacterial inoculation significantly influenced soil microbial activity by modifying respiration rates and hydrolytic enzyme intensity in plant- and manure-specific ways. The results demonstrate that microbial supplementation can alter soil biological processes and nutrient turnover, though its effects on plant productivity remain inconsistent. Further research is required to assess long-term performance under field conditions, as practical application will depend on achieving stable and reproducible results. Full article

Ex-ante appraisal of agricultural policy needs a transparent way to trace how sectoral interventions translate into production. We study the Polish CAP case and ask how much selected actions matter for livestock sectors. We assembled intervention-level budgets from the CAP Strategic Plan for Poland (2023–2027) and sectoral final output for milk, pigs, beef and poultry from Statistics Poland/Eurostat. We built matrices that map actions to sectors, normalized transfers by sectoral output, and separated dedicated from spillover effects. We report two cross-sections (2024, 2028) and a robustness test that perturbs I 1–I 2 allocation shares by ±10% under fixed envelopes. Horizontal income support dominates. In 2024, the cumulative effect of all analyzed actions equaled 16.68% of final output in milk, 14.43% in beef, 5.15% in pigs and 4.29% in poultry; by 2028, these values ease to 15.07%, 12.93%, 3.84% and 4.15%. Coupled payments to cows and young cattle add contributions in milk and beef. The ±10% reweighting of I 1–I 2 keeps the sector ranking unchanged; level changes are moderate (about 0.4–1.2 percentage points). A compact matrix approach provides a replicable map from interventions to sectors and highlights the preponderance of horizontal income support. The pattern—strongest relative support in milk and beef—appears robust to plausible allocation uncertainty. The main limitation is the use of final output as a revenue proxy; extending the matrix to all CAP actions and adding price–quantity feedback would be a natural next step. Policy implication: modest rebalancing of I 1–I 2 shares will not overturn sectoral exposure, but adjustments targeted at beef move levels the most. Full article

Background: Foot-and-mouth disease (FMD) is a highly contagious viral disease of cloven-hoofed animals such as cattle and pigs, characterized by vesicular lesions in the mouth, nose, teats, and feet. Globally, the most commonly used FMD vaccines are inactivated vaccines produced by chemical inactivation of the infectious FMD virus (FMDV). This study aimed to establish an infectious clone of the O/Boeun/SKR/2017 virus that has demonstrated the highest antigen productivity among the various type O vaccine strains developed in South Korea to date. Methods: An infectious clone was generated from a type O virus isolated during the 2017 FMD outbreak in South Korea. The viral genome was divided into two fragments, each amplified separately, and subsequently ligated to produce a full-length infectious clone. Results: Rescue of infectious FMDV was confirmed using a commercial antigen detection kit and electron microscopy. Under optimized culture conditions, the rescued virus titer reached 2 × 10⁷ TCID₅₀/mL, and the antigen yield was 6.4 µg/mL. Following inactivation, the antigen was formulated into a vaccine and administered to pigs. Four weeks post-vaccination, challenge with the live virus resulted in no clinical symptoms, demonstrating complete protective efficacy. Conclusions: To the best of our knowledge, this is the first report describing the construction of an infectious clone derived from a field FMDV isolate in South Korea and its application in vaccine development. The O/Boeun/SKR/2017 infectious clone may serve as a genetic backbone for the rapid generation of new FMD vaccine candidates with high antigen productivity by substituting epitopes from other FMDV. Full article

Enterocytozoon bieneusi is the most frequently diagnosed microsporidian parasite in humans and a recognized cause of diarrheal disease, particularly in immunocompromised individuals. Its broad host range, which includes livestock, companion animals, and wildlife, highlights its zoonotic potential and warrants careful epidemiological assessment. This narrative review synthesizes available data on the occurrence and genetic diversity of E. bieneusi in European domestic ungulates (cattle, pigs, sheep, goats, horses, and water buffaloes) and pets (dogs and cats), aiming to provide an integrated perspective on animal reservoirs and their relevance for public health. Publications retrieved from the Web of Science Core Collection database were systematically screened, and country-specific results were extracted, emphasizing prevalence rates, genotype distributions, and zoonotic implications. Across Europe, cattle and pigs emerged as the most studied hosts, frequently harboring zoonotic group 1 genotypes such as I, J, BEB4, BEB6, and EbpA, while small ruminants, horses, and buffaloes remain comparatively undocumented. In pets, the dog-adapted genotype PtEb IX was predominant, but several zoonotic genotypes were also identified. Overall, the current evidence confirms the wide host range of E. bieneusi in Europe but also reveals significant data gaps compared to regions such as China, underlining the need for broader surveillance and harmonized molecular approaches within a One Health framework. Full article

Background/Objectives: In an era of increasing bacterial resistance, Enterococcus, as a reservoir of antibiotic resistance genes, poses a serious threat to public health. Methods: This study conducted antibiotic susceptibility tests, whole-genome sequencing, and bioinformatics analysis on 89 Enterococcus isolates from chickens, pigs, cattle, and sheep in Ningxia Autonomous Region. Results: The resistance rates of Enterococcus to clindamycin, cefoxitin, sulfamethoxazole, and tamoxifen were all above 95%, and 96.6% (86/89) of the isolates were multi-antibiotic resistant. There were significant differences in resistance phenotypes among different species, with Enterococcus from pigs showing significantly higher resistance than those from other animals. optrA was commonly found in Enterococcus from pigs, accounting for 61.5% (8/13). ST480, ST16, ST116, and ST300 were the main MLST types, and ST16 was one of the important pathogenic Enterococcus types. Conclusions: The study revealed the occurrence of inter-species transmission events of Enterococcus. In conclusion, this study comprehensively described the resistance spectrum, sequence characteristics, and transmission features of resistance genes in Enterococcus isolated from farm animals, and emphasized the possibility of the spread of resistance genes carried by Enterococcus from farm animals to humans. Full article

Long-term fertilization affects soil nutrient levels and aggregate stability, eventually altering crop yield. However, their responses to organic fertilizer application and straw returning are still unclear, particularly as the contributions of soil nutrient levels and aggregate stability on crop yields remain poorly quantified. Therefore, topsoil samples (0–20 cm) were collected from six fertilization treatments in a long-term (13-year) Shajiang black soil field experiment with no fertilization (CK), chemical fertilization (NPK), 50% NPK plus pig manure (50%NPKP), 50% NPK plus cattle manure (50%NPKC), 70% NPK plus pig manure with straw return (70%NPKPS), and 70% NPK plus cattle manure with straw return (70%NPKCS). We examined the characteristics of crop yield, soil nutrient levels, and soil aggregate stability parameters, including under different long-term fertilization treatments. The results show that long-term fertilization significantly influenced the distribution of soil nutrients and soil aggregates in Shajiang black soil. Compared to CK, organic fertilizers and straw returning significantly increased the soil organic matter (SOM), total nitrogen (TN), and total phosphorus (TP) contents but decreased soil pH, respectively, indicating the best strategies for improving soil fertility. Compared to the CK and NPK treatments, long-term organic fertilization and straw returning significantly increased the mean weight diameter (MWD) and geometric mean diameter (GMD) values and significantly decreased the fractal dimension (Dm) and mean weight-specific surface area (MWSSA) values, with the 70%NPKCS treatment showing the most pronounced effect of improving aggregate stability. A redundancy analysis revealed that SOM and TN exert significant effects on aggregate stability. Furthermore, a stepwise regression analysis showed that SOM and TN were positive factors affecting the yields of wheat and maize, while MWD and pH were negative factors affecting wheat yield, demonstrating that high crop yields are derived from soils with limited stability and high fertility. Thus, our findings indicate that the integrated application of cattle manure with straw returning was the most effective strategy to promote soil nutrient accumulation, improve aggregate stability, and enhance crop yield, albeit with the potential risk of soil acidification, which requires management in the Shajiang black soil (Vertisol) region of Northern China. Full article

This work proposes to contribute through an interdisciplinary perspective to the evaluation of paleoeconomic and paleoenvironmental changes during Middle Holocene in Southwestern Transylvania. The study integrates archaeozoological data with phytolith analysis to reconstruct subsistence and vegetation dynamics from the Early Neolithic to the Late Bronze Age at Șoimuș-Teleghi (Hunedoara County, Romania). Animal remains are described in terms of their frequency (i.e., number of identified specimens and minimum number of individuals), taphonomic changes, and livestock management (i.e., animal selection by age and sex). Archaeozoological samples are dominated by skeletal remains from domestic mammals (e.g., cattle, sheep/goat, and pig), whose importance varies depending on the cultural level; the skeletal remains of wild mammals are less frequent, mainly belonging to species with large size (e.g., red deer, wild boar, roe deer, aurochs). This study tests whether animal exploitation strategies shifted from ruminant-dominated economies in the Neolithic to greater pig reliance in the Bronze Age, using the Shannon–Weaver diversity index and correspondence analysis. Phytolith analysis of eleven sediment samples from various cultural layers reveals the dominance of Pooideae-type grasses, with both vegetative plant parts and cereal inflorescences as resources. Bioarchaeological data presented in this study reveal a diachronic shift in subsistence practices, reflecting cultural and environmental transformations. Full article