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To address the issue that existing raisin foreign object removal equipment cannot eliminate surface contaminants adhered to raisins through non-washing methods, this paper proposes an adhesive foreign object removal method based on “rapid freezing–rolling extrusion separation-airflow screening”. A raisin adhesive foreign object removal device was designed based on this method. The separation and removal processes of adhesive foreign objects were analyzed and optimized through simulation, followed by device fabrication and performance testing. Starting from the separation process of raisins and adhesive foreign objects, we conducted experimental studies on quick-freezing separation, determined the most suitable separation method based on experimental results, and performed structural design of the equipment accordingly. To conduct simulation analysis and optimization, material parameters were calibrated. The working process of foreign object separation was simulated and optimized using discrete element method (DEM) simulation, verifying the equipment’s separation capability for different adhesive foreign objects while determining the optimal rotational speed of 600 r/min. Through EDEM-Fluent coupled simulation, the working process of foreign object removal was analyzed and optimized, validating the influence of flow field on foreign object removal and determining the optimal air velocity of 11 m/s. The equipment was ultimately fabricated, with further parameter optimization and comprehensive performance testing conducted. The final optimal rotational speed and air velocity were determined as 650 r/min and 11 m/s, respectively. In terms of comprehensive performance, the equipment achieved a separation rate of 93.76%, damage rate of 3.05%, residue rate of 4.28%, removal rate of 94.52%, carry-over ratio of 71:1, and processing capacity of 120 kg/h. Full article

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14 pages, 3011 KiB

Open AccessArticle

Ameliorative Effects of Soybean Powder Fermented by Bacillus subtilis on Constipation Induced by Loperamide in Rats

by Gi Soo Lee, Su Kang Kim, Ju Yeon Ban and Chung-Hun Oh

Int. J. Mol. Sci. 2025, 26(15), 7615; https://doi.org/10.3390/ijms26157615 (registering DOI) - 6 Aug 2025

Abstract

Constipation is a prevalent gastrointestinal disorder that significantly impairs quality of life. While pharmacological agents such as loperamide are widely used to induce constipation in experimental models, there is increasing interest in natural alternatives for alleviating intestinal dysfunction. In this study, we investigated the laxative effects of soybean powder fermented by Bacillus subtilis DKU_09 in a loperamide-induced rat model of constipation. The probiotic strain was isolated from cheonggukjang, a traditional Korean fermented soybean paste, and its identity was confirmed through 16S rRNA sequencing. Fermented soybean powder was characterized morphologically via scanning electron microscopy and chemically via HPLC to assess its isoflavone content. Rats were administered loperamide (5 mg/kg) for four days to induce constipation and were then treated with fermented soybean powder at doses of 100, 200, or 300 mg/kg. No pharmacological laxatives (e.g., PEG) were used as a positive control; instead, values from the treatment groups were compared with those from the loperamide-only constipation group. Key outcomes of fecal output, water content, colonic fecal retention, and gastrointestinal transit ratio were measured. The fermented product significantly improved stool frequency and moisture content, reduced colonic fecal retention, and restored gastrointestinal transit in a dose-dependent manner. Notably, the 300 mg/kg group demonstrated nearly complete recovery of fecal parameters without affecting body weight. Statistical analysis was performed using one-way ANOVA followed by Tukey’s post hoc test. These findings suggest that Bacillus subtilis-fermented soybean powder exerts synergistic laxative effects through the combined action of probiotic viability and fermentation-enhanced bioactive compounds such as aglycone isoflavones. This study supports the potential use of fermented soybean-based nutraceuticals as a natural and safe intervention for constipation and gastrointestinal dysregulation. Full article

(This article belongs to the Special Issue Functions and Applications of Natural Products)

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18 pages, 11555 KiB

Open AccessArticle

Impacts of Land Use and Hydrological Regime on the Spatiotemporal Distribution of Ecosystem Services in a Large Yangtze River-Connected Lake Region

by Ying Huang, Xinsheng Chen, Ying Zhuo and Lianlian Zhu

Water 2025, 17(15), 2337; https://doi.org/10.3390/w17152337 (registering DOI) - 6 Aug 2025

Abstract

In river-connected lake regions, both land use and hydrological regime changes may affect the ecosystem services; however, few studies have attempted to elucidate their complex influences. In this study, the spatiotemporal dynamics of eight ecosystem services (crop production, aquatic production, water yield, soil retention, flood regulation, water purification, net primary productivity, and habitat quality) were investigated through remote-sensing images and the InVEST model in the Dongting Lake Region during 2000–2020. Results revealed that crop and aquatic production increased significantly from 2000 to 2020, particularly in the northwestern and central regions, while soil retention and net primary productivity also improved. However, flood regulation, water purification, and habitat quality decreased, with the fastest decline in habitat quality occurring at the periphery of the Dongting Lake. Land-use types accounted for 63.3%, 53.8%, and 40.3% of spatial heterogeneity in habitat quality, flood regulation, and water purification, respectively. Land-use changes, particularly the expansion of construction land and the conversion of water bodies to cropland, led to a sharp decline in soil retention, flood regulation, water purification, net primary productivity, and habitat quality. In addition, crop production and aquatic production were higher in cultivated land and residential land, while the accompanying degradation of flood regulation, water purification, and habitat quality formed a “production-pollution-degradation” spatial coupling pattern. Furthermore, hydrological fluctuations further complicated these dynamics; wet years amplified agricultural outputs but intensified ecological degradation through spatial spillover effects. These findings underscore the need for integrated land-use and hydrological management strategies that balance human livelihoods with ecosystem resilience. Full article

(This article belongs to the Section Ecohydrology)

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22 pages, 775 KiB

Open AccessReview

Bioactive Compounds, Technological Advances, and Sustainable Applications of Avocado (Persea americana Mill.): A Critical Review

by Amanda Priscila Silva Nascimento, Maria Elita Martins Duarte, Ana Paula Trindade Rocha and Ana Novo Barros

Foods 2025, 14(15), 2746; https://doi.org/10.3390/foods14152746 (registering DOI) - 6 Aug 2025

Abstract

Avocado (Persea americana), originally from Mesoamerica, has emerged as a focus of intense scientific and industrial interest due to its unique combination of nutritional richness, bioactive potential, and technological versatility. Its pulp, widely consumed across the globe, is notably abundant in monounsaturated fatty acids, especially oleic acid, which can comprise over two-thirds of its lipid content. In addition, it provides significant levels of dietary fiber, fat-soluble vitamins such as A, D, E and K, carotenoids, tocopherols, and phytosterols like β-sitosterol. These constituents are consistently associated with antioxidant, anti-inflammatory, glycemic regulatory, and cardioprotective effects, supported by a growing body of experimental and clinical evidence. This review offers a comprehensive and critical synthesis of the chemical composition and functional properties of avocado, with particular emphasis on its lipid profile, phenolic compounds, and phytosterols. It also explores recent advances in environmentally sustainable extraction techniques, including ultrasound-assisted and microwave-assisted processes, as well as the application of natural deep eutectic solvents. These technologies have demonstrated improved efficiency in recovering bioactives while aligning with the principles of green chemistry. The use of avocado-derived ingredients in nanostructured delivery systems and their incorporation into functional foods, cosmetics, and health-promoting formulations is discussed in detail. Additionally, the potential of native cultivars and the application of precision nutrition strategies are identified as promising avenues for future innovation. Taken together, the findings underscore the avocado’s relevance as a high-value matrix for sustainable development. Future research should focus on optimizing extraction protocols, clarifying pharmacokinetic behavior, and ensuring long-term safety in diverse applications. Full article

(This article belongs to the Special Issue Feature Review on Nutraceuticals, Functional Foods, and Novel Foods)

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11 pages, 327 KiB

Open AccessArticle

Metabolic Mediation of the Association Between Hyperandrogenism and Paratubal Cysts in Polycystic Ovary Syndrome: A Structural Equation Modeling Approach

by Jin Kyung Baek, Chae Eun Hong, Hee Yon Kim and Bo Hyon Yun

J. Clin. Med. 2025, 14(15), 5545; https://doi.org/10.3390/jcm14155545 (registering DOI) - 6 Aug 2025

Abstract

Objectives: Paratubal cysts (PTCs) are embryological remnants and are potentially hormonally responsive. Since hyperandrogenism (HA) is representative of polycystic ovary syndrome (PCOS), we examined whether biochemical hyperandrogenism is associated with PTCs in women with PCOS and if body mass index (BMI) and insulin resistance (IR) mediate this association. Methods: This retrospective study included 577 women diagnosed with PCOS at a tertiary academic center from 2010 to 2018. Clinical data included age at diagnosis, BMI, and diagnoses of hypertension, non-alcoholic fatty liver disease, and metabolic syndrome. Laboratory measures included total testosterone, sex hormone-binding globulin, anti-Müllerian hormone, luteinizing hormone, fasting glucose, insulin, and triglycerides (TG). Derived indices included a free androgen index (FAI), homeostasis model assessment of insulin resistance (HOMA-IR), and fasting glucose-to-insulin ratio. PTCs were identified through imaging or surgical findings. Structural equation modeling (SEM) assessed direct and indirect relationships between FAI, BMI, HOMA-IR, and PTCs, while adjusting for diagnostic age. Results: PTCs were identified in 2.77% of participants. BMI, FAI, TG, and IR indices were significantly higher for women with PTCs than those without PTCs. SEM revealed significant indirect effects of FAI on PTCs via BMI and HOMA-IR. The direct effect was negative, resulting in a non-significant total effect. A sensitivity model using HOMA-IR as the predictor showed a significant direct effect on PTCs without mediation via FAI. Conclusions: Biochemical HA may influence PTC development in PCOS through metabolic pathways, establishing the need to consider metabolic context when evaluating adnexal cysts in hyperandrogenic women. Full article

(This article belongs to the Special Issue Clinical Perspectives on Women’s Reproductive Health: Challenges and Management Strategies)

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16 pages, 1298 KiB

Open AccessArticle

Genetic Effects of Chicken Pre-miR-3528 SNP on Growth Performance, Meat Quality Traits, and Serum Enzyme Activities

by Jianzhou Shi, Jinbing Zhao, Bingxue Dong, Na Li, Lunguang Yao and Guirong Sun

Animals 2025, 15(15), 2300; https://doi.org/10.3390/ani15152300 (registering DOI) - 6 Aug 2025

Abstract

The aim was to investigate the genetic effects of a SNP located in the precursor region of gga-miR-3528. (1) Single-nucleotide polymorphisms within precursor regions of microRNAs play crucial biological roles. (2) Utilizing a Gushi–Anka F₂ resource population (n = 860), we screened and validated miRNA SNPs. A SNP mutation in the miR-3528 precursor region was identified. Specific primers were designed to amplify the polymorphic fragment. Genotyping was performed for this individual SNP across the population, using the MassArray system. Association analyses were conducted between this SNP and chicken growth and body measurement traits, carcass traits, meat quality traits, and serum enzyme activities. (3) The rs14098602 (+12 bp A > G) was identified within the precursor region of gga-miR-3528. Significant associations (p < 0.05) were observed between this SNP and chicken growth traits (body weight at the age of 0 day, body weight at the age of 2 weeks, and body weight at the age of 4 weeks), carcass traits (evisceration weight), meat quality traits (subcutaneous fat rate and pectoral muscle density), and serum enzyme activities (total protein, albumin, globulin, cholinesterase, and lactate dehydrogenase). (4) These findings suggest that the polymorphism at rs14098602 may influence chicken growth, meat quality, and serum biochemical indices, through specific mechanisms. The gga-miR-3528 gene likely plays an important role in chicken development. Therefore, this SNP can serve as a molecular marker for genetic breeding and auxiliary selection of growth-related traits, facilitating the rapid establishment of elite chicken populations with superior genetic resources. Full article

(This article belongs to the Section Poultry)

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33 pages, 891 KiB

Open AccessArticle

Effectiveness of a Mind–Body Intervention at Improving Mental Health and Performance Among Career Firefighters

by Anthony C. Santos, Seth Long, Christopher P. Moreno and Dierdra Bycura

Int. J. Environ. Res. Public Health 2025, 22(8), 1227; https://doi.org/10.3390/ijerph22081227 (registering DOI) - 6 Aug 2025

Abstract

Almost one in three firefighters develop mental health disorders at some point during their careers, a rate double that in the general population. Frequent exposures to potentially traumatic situations can contribute to symptoms of these disorders, two of the most common being depression and post-traumatic stress disorder (PTSD). While various psychological interventions have been implemented among this group, reports of their effectiveness include mixed results. To this end, the current study endeavored to test the effectiveness of a 12-week intervention combining occupationally-tailored high-intensity functional training (HIFT) and psychological resilience training (RES) in reducing depressive and post-traumatic stress symptoms (PTSSs), as well as increasing psychological resilience and mental wellbeing, in career firefighters. Thirty career firefighters completed four mental health measurements over 17 weeks while anthropometrics and physical performance (i.e., number of stations completed in 20 min during an eight-station simulated job-task circuit workout [T-CAC]) were measured pre- and post-intervention. Pre to post comparisons were made via repeated-measures t-tests. Significant mean differences were observed for T-CAC stations completed, PTSSs, and psychological resilience between pre- and post-intervention. In future interventions, researchers should actively engage firefighters, maximize integration with daily operations, and employ culturally-relevant practices to explore the links between physical and mental health. Full article

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20 pages, 1119 KiB

Open AccessArticle

Smartphone-Assisted Experimentation as a Medium of Understanding Human Biology Through Inquiry-Based Learning

by Giovanna Brita Campilongo, Giovanna Tonzar-Santos, Maria Eduarda dos Santos Verginio and Camilo Lellis-Santos

Educ. Sci. 2025, 15(8), 1005; https://doi.org/10.3390/educsci15081005 (registering DOI) - 6 Aug 2025

Abstract

The integration of Inquiry-Based Learning (IBL) and mobile technologies can transform science education, offering experimentation opportunities to students from budget-constrained schools. This study investigates the efficacy of smartphone-assisted experimentation (SAE) within IBL to enhance pre-service science teachers’ understanding of human physiology and presents a newly developed and validated rubric for assessing their scientific skills. Students (N = 286) from a Science and Mathematics Teacher Education Program participated in a summative IBL activity (“Investigating the Human Physiology”—iHPhys) where they designed experimental projects using smartphone applications to collect body sign data. The scoring rubric, assessing seven criteria including hypothesis formulation, methodological design, data presentation, and conclusion writing, was validated as substantial to almost perfect inter-rater reliability. Results reveal that students exhibited strong skills in hypothesis clarity, theoretical grounding, and experimental design, with a high degree of methodological innovation observed. However, challenges persisted in predictive reasoning and evidence-based conclusion writing. The students were strongly interested in inquiring about the cardiovascular and nervous systems. Correlational analyses suggest a positive relationship between project originality and overall academic performance. Thus, integrating SAE and IBL fosters critical scientific competencies, creativity, and epistemic cognition while democratizing access to scientific experimentation and engaging students in tech-savvy pedagogical practices. Full article

(This article belongs to the Special Issue Inquiry-Based Learning and Student Engagement)

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14 pages, 649 KiB

Open AccessArticle

Investigating the Moderating Effect of Attitudes Toward One’s Own Aging on the Association Between Body Mass Index and Executive Function in Older Adults

by Akihiko Iwahara, Taketoshi Hatta, Reiko Nakayama, Takashi Miyawaki, Seiji Sakate, Junko Hatta and Takeshi Hatta

Geriatrics 2025, 10(4), 105; https://doi.org/10.3390/geriatrics10040105 (registering DOI) - 6 Aug 2025

Abstract

Background: This cross-sectional study examined the association between body mass index (BMI) and executive function (EF) in older adults, with a focus on the moderating role of attitudes toward own aging (ATOA). Method: A total of 431 community-dwelling elderly individuals from Yakumo Town and Kyoto City, Japan, participated between 2023 and 2024. EF was assessed using the Digit Cancellation Test (D-CAT), and ATOA was measured via a validated subscale of the Philadelphia Geriatric Center Morale Scale. Results: Multiple linear regression analyses adjusted for demographic and health covariates revealed a significant interaction between BMI and ATOA in the younger-old cohort. Specifically, higher BMI was associated with lower executive function only in individuals with lower ATOA scores. No such association was observed in those with more positive views on aging. Conclusions: These results indicate that positive psychological constructs, particularly favorable self-perceptions of aging, may serve as protective factors against the detrimental cognitive consequences of increased body mass index in younger-old populations. Full article

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12 pages, 486 KiB

Open AccessArticle

Efficacy and Safety of Dose-Dense Chemotherapy in Breast Cancer: Real Clinical Data and Literature Review

by Keiko Yanagihara, Masato Yoshida, Tamami Yamakawa, Sena Kato, Miki Tamura and Koji Nagata

Curr. Oncol. 2025, 32(8), 441; https://doi.org/10.3390/curroncol32080441 (registering DOI) - 6 Aug 2025

Abstract

Dose-dense chemotherapy shortens the interval between chemotherapy cycles and has shown improved outcomes in high-risk breast cancer patients. We retrospectively evaluated the efficacy and safety of dose-dense chemotherapy in 80 breast cancer patients treated at our hospital from 2020 to 2024. The regimen included epirubicin and cyclophosphamide followed by paclitaxel or docetaxel, with pegfilgrastim support. The overall treatment completion rate was 82.5%. Of the 80 patients, 55 underwent neoadjuvant chemotherapy, and the pathological complete response rate was significantly higher in triple-negative breast cancer (59.1%) compared to that in luminal-type cancer (9.1%). Common adverse events included anemia, liver dysfunction, myalgia, and peripheral neuropathy. Febrile neutropenia occurred in 8.8% of patients, with some cases linked to pegfilgrastim body pod use, particularly in individuals with low subcutaneous fat. Notably, two patients developed pneumocystis pneumonia, potentially associated with steroid administration. Despite these toxicities, most were manageable and resolved after treatment. Our findings support the efficacy of dose-dense chemotherapy, particularly in triple-negative breast cancer, while highlighting the importance of individualized supportive care and vigilance regarding hematologic and infectious complications. Full article

(This article belongs to the Special Issue From Screening to Treatment: Technology’s Impact on Breast Cancer Care)

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28 pages, 3613 KiB

Open AccessReview

Epigenetic Alterations in Age-Related Macular Degeneration: Mechanisms and Implications

by Dana Kisswani, Christina Carroll, Fatima Valdes-Mora and Matt Rutar

Int. J. Mol. Sci. 2025, 26(15), 7601; https://doi.org/10.3390/ijms26157601 (registering DOI) - 6 Aug 2025

Abstract

Age-related macular degeneration (AMD) is one of the leading causes of irreversible vision loss among the elderly, and is influenced by a combination of genetic and environmental risk factors. While genetic associations in AMD are well-established, the molecular mechanisms underlying disease onset and progression remain poorly understood. A growing body of evidence suggests that epigenetic modifications may serve as a potential missing link regulating gene–environment interactions. This review incorporates recent findings on DNA methylation, including both hypermethylation and hypomethylation patterns affecting genes such as silent mating type information regulation 2 homolog 1 (SIRT1), glutathione S-transferase isoform (GSTM), and SKI proto-oncogene (SKI), which may influence key pathophysiological drivers of AMD. We also examine histone modification patterns, chromatin accessibility, the status of long non-coding RNAs (lncRNAs) in AMD pathogenesis and in regulating pathways pertinent to the pathophysiology of the disease. While the field of ocular epigenetics remains in its infancy, accumulating evidence to date points to a burgeoning role for epigenetic regulation in AMD, pre-clinical studies have yielded promising findings for the prospect of epigenetics as a future therapeutic avenue. Full article

(This article belongs to the Special Issue Research Advances in Retinal Neurodegeneration and Neuroprotection Strategies)

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16 pages, 1541 KiB

Open AccessArticle

A Ubiquitous Volatile in Noctuid Larval Frass Attracts a Parasitoid Species

by Chaowei Wang, Xingzhou Liu, Sylvestre T. O. Kelehoun, Kai Dong, Yueying Wang, Maozhu Yin, Jinbu Li, Yu Gao and Hao Xu

Biology 2025, 14(8), 1007; https://doi.org/10.3390/biology14081007 (registering DOI) - 6 Aug 2025

Abstract

Natural enemies commonly probe larval bodies and frass with their antennae for prey hunting. However, the attractants to natural enemies emitted directly from hosts and host-associated tissues remained largely unknown. Here, we used two generalist noctuid species, Helicoverpa armigera (Hübner) and Spodoptera frugiperda (JE Smith), along with the larval endoparasitoid Microplitis mediator (Haliday) to address the question. Extracts of larval frass of both the noctuid species were strongly attractive to M. mediator females when hosts were fed either maize, cotton, soybean leaves, or an artificial diet without leaf tissues. By using a combination of electrophysiological measurements and behavioral tests, we found that the attractiveness of frass mainly relied on a volatile compound ethyl palmitate. The compound was likely to be a by-product of host digestion involving gut bacteria because an antibiotic supplement in diets reduced the production of the compound in frass and led to the decreased attractiveness of frass to the parasitoids. In contrast, extracts of the larval bodies of both the noctuid species appeared to be less attractive to the parasitoids than their respective fecal extracts, independently of types of food supplied to the larvae. Altogether, larval frass of the two noctuid species was likely to be more important than their bodies in attracting the endoparasitoid species, and the main attractant of frass was probably one of the common metabolites of digestion involving gut microbes, and its emission is likely to be independent of host plant species. Full article

(This article belongs to the Special Issue The Biology, Ecology, and Management of Plant Pests)

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18 pages, 1528 KiB

Open AccessReview

Sex Differences in Colorectal Cancer: Epidemiology, Risk Factors, and Clinical Outcomes

by Sophia Tsokkou, Ioannis Konstantinidis, Menelaos Papakonstantinou, Paraskevi Chatzikomnitsa, Eftychia Liampou, Evdokia Toutziari, Dimitrios Giakoustidis, Petros Bangeas, Vasileios Papadopoulos and Alexandros Giakoustidis

J. Clin. Med. 2025, 14(15), 5539; https://doi.org/10.3390/jcm14155539 (registering DOI) - 6 Aug 2025

Abstract

Colorectal cancer (CRC) constitutes a major global health concern, ranking as the third most common cancer and the second leading cause of cancer-related mortality. The current review explores sex-based differences in CRC epidemiology, risk factors, tumor biology, and clinical outcomes. Males exhibit a higher incidence and mortality rate, with left-sided (distal) CRC predominating, while females are more frequently diagnosed with right-sided (proximal) tumors, which tend to be more aggressive and less responsive to conventional chemotherapy. Genetic disparities, including microsatellite instability and X-chromosome tumor suppressor genes, contribute to sex-specific differences in tumor progression and treatment response. Immune variations also influence disease outcomes, with females exhibiting stronger immune surveillance but higher exhaustion markers. Lifestyle factors such as body mass index (BMI), smoking, and hormonal influences further modulate CRC risk. While males are more vulnerable to obesity-related CRC, central obesity (waist-to-hip ratio) emerges as a stronger predictor in females. Additionally, smoking increases CRC risk differentially by tumor location. These findings underscore the importance of sex-specific approaches in CRC prevention, screening, and treatment, advocating for personalized medicine strategies tailored to gender-based biological and clinical distinctions. Full article

(This article belongs to the Special Issue Gastrointestinal Cancer: Outcomes and Therapeutic Management)

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19 pages, 1102 KiB

Open AccessArticle

Assessing the Adoption and Feasibility of Green Wall Systems in Construction Projects in Nigeria

by Oluwayinka Seun Oke, John Ogbeleakhu Aliu, Damilola Ekundayo, Ayodeji Emmanuel Oke and Nwabueze Kingsley Chukwuma

Sustainability 2025, 17(15), 7126; https://doi.org/10.3390/su17157126 (registering DOI) - 6 Aug 2025

Abstract

This study aims to evaluate the level of awareness and practical adoption of green wall systems in the Nigerian construction industry. It seeks to examine the current state of green wall implementation and recommend strategies to enhance their integration into construction practices among Nigerian construction professionals. A thorough review of the existing literature was conducted to identify different types of green wall systems. Insights from this review informed the design of a structured questionnaire, which was distributed to construction professionals based in Lagos State. The data collected were analyzed using statistical tests. The study reveals that while there is generally high awareness of green wall systems among Nigerian construction professionals, the practical use remains low, with just 8 out of the 18 systems being actively implemented, eclipsing the mean value of 3.0. The findings underscore the need for targeted education, industry incentives, and increased advocacy to encourage the use of green wall systems in the Nigerian construction sector. The results have significant implications for the Nigerian construction industry. The limited awareness and adoption of green wall systems highlight the need for strategic actions from policymakers, industry leaders and educational institutions. Promoting the use of green walls could drive more sustainable building practices, improve environmental outcomes and support the broader goals of decarbonization and circularity in construction. This research adds to the body of knowledge on sustainable construction by offering a detailed evaluation of green wall awareness and adoption within the Nigerian context. While green wall systems have been studied globally, this research provides a regional perspective, which in this case focuses on Lagos State. The study’s recognition of the gap between awareness and implementation highlights an important area for future research and industry development. Full article

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13 pages, 657 KiB

Open AccessArticle

Physical Fitness, Experiential Avoidance, and Psychological Inflexibility Among Adolescents: Results from the EHDLA Study

by Maria Mendoza-Muñoz, José Francisco López-Gil, Damián Pereira-Payo and Raquel Pastor-Cisneros

Children 2025, 12(8), 1032; https://doi.org/10.3390/children12081032 (registering DOI) - 6 Aug 2025

Abstract

Background/Introduction: Psychological inflexibility, which includes experiential avoidance, is a transdiagnostic process associated with multiple mental health issues in adolescence. Physical fitness (PF) has shown benefits for mental well-being, yet its specific relationship with psychological inflexibility remains understudied, particularly among youth. Objectives: To examine the association between components of PF and psychological inflexibility, measured by the Acceptance and Action Questionnaire-II (AAQ-II), in a representative sample of Spanish adolescents. Methods: A cross-sectional analysis was conducted using data from 631 adolescents (aged 12–17) participating in the Eating Healthy and Daily Life Activities (EHDLA) study. PF was assessed by the Assessing the Levels of PHysical Activity and Fitness (ALPHA-Fit) Test Battery (cardiorespiratory fitness, muscular strength, agility, and flexibility). Psychological inflexibility was measured using the AAQ-II. Generalized linear models (GLMs) were used to evaluate associations, adjusting for age, sex, body mass index, socioeconomic status, physical activity, sedentary behavior, sleep duration, and energy intake. Results: Unadjusted analyses showed weak but significant associations between psychological inflexibility and performance in the 20 m shuttle run test (p = 0.002), the 4 × 10 shuttle run test (p = 0.005), and the sit-and-reach test (p < 0.001). However, after adjusting for covariates, none of the PF components maintained a statistically significant association with the AAQ-II scores. Conclusions: In this adolescent sample, PF components were not independently associated with psychological inflexibility after adjustment for key confounders. These findings suggest that, while PF may contribute to general well-being, it is not a primary determinant of psychological inflexibility. Further longitudinal and intervention studies are needed to clarify the mechanisms linking physical and psychological health in youth. Full article

(This article belongs to the Special Issue Physical Fitness and Health in Adolescents)

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