Search Results (243)

Electrophysiological studies reveal significant organizational and functional differences in the cortex of blind individuals compared to sighted individuals. These differences result from the nervous system’s reorganization to adapt to new sensory modalities used in daily life. Cortical visual prostheses offer a means to restore visual sensations in blind individuals by generating phosphenes, luminous perceptions that provide information about their surroundings. This study investigates the cortical changes associated with the use of a visual neuroprosthesis, focusing on how the brain adapts to the restored visual input. Our findings aim to contribute to understanding neuroplasticity in sensory restoration processes. Full article

Gene therapy has emerged as a promising treatment for several eye diseases since it may restore vision and stop blindness. Many eye diseases, including retinitis pigmentosa and macular degeneration, have historically been rather difficult to treat and usually cause permanent vision loss. However, thanks to advances in gene therapy, many disorders can now be effectively targeted and genetically changed, providing a safer, more direct, maybe even curative approach. By introducing, altering, or repairing specific genes inside the eye, gene therapy seeks to fix the defective genes causing these disorders, thereby improving general eye health and visual ability. Voretigene neparvovec is one FDA- and EMA-approved treatment for RPE65 mutations. Retinitis pigmentosa, age-related macular degeneration, X-linked retinoschisis, choroideremia, and Stargardt disease are among the several eye disorders still under clinical trials, and experimental treatment is in progress. As research on gene therapy develops, it opens the path for groundbreaking treatments that could fundamentally change the ophthalmic care scene. Full article

Cataracts remain one of the leading causes of reversible blindness in low- and middle-income countries such as Ecuador. This study assessed the efficacy of Small Incision Cataract Surgery (SICS) and analyzed sociodemographic and clinical factors associated with postoperative visual outcomes. A retrospective multicenter analysis was conducted across six ophthalmology clinics along the Ecuadorian coast between 2023 and 2024, including 558 patients aged 30 years or older. Postoperative visual acuity, measured using the LogMAR scale, improved significantly (mean improvement of 0.525 LogMAR units in the right eye (OD) and 0.489 LogMAR units in the left eye; p < 0.001). Ages between 60 and 69 years were associated with better outcomes in the right eye, while male sex was a protective factor against poor visual acuity in the left eye. Although diabetes mellitus and hypertension were prevalent, neither condition showed a significant association with postoperative visual outcomes. The findings confirm that SICS is a safe, effective, and cost-efficient surgical approach for restoring vision in resource-limited settings, supporting its inclusion in national public health strategies to reduce avoidable blindness in developing countries. Full article

Age-related macular degeneration (AMD) is a common cause of blindness worldwide, and it is projected to affect several million individuals by 2040. The human retinal pigment epithelium (hRPE) degenerates in dry AMD, prompting the need to develop stem cell therapies to replace the lost tissue by autologous transplantation and restore the visual function. Nevertheless, the molecular factors behind the hRPE cell fate determination have not been elucidated. Here we identify all molecular determinants of the hRPE cell fate identity by comprehensive and unbiased screening of predicted pioneer factors in the human genome: such TFs mediate coordinated transitions in chromatin accessibility and transcriptional outcome along three major stages of the hRPE genesis. Furthermore, we compile a complete census of all transcription factor-specific binding sites by footprinting analysis of the human epigenome along the RPE developmental trajectory. Gene regulatory networks were found to be involved in cellular responses to glucose and hypoxia, RPE nitrosative stress, type II epithelial-to-mesenchymal transition (EMT), and type III tumorigenic EMT, providing routes for therapeutic intervention on pleiotropic targets dysregulated in AMD, diabetic retinopathy, and cancer progression. Genome editing technologies may leverage this repository to devise functional screenings of regulatory elements and pharmacogenomic therapies in complex diseases, paving the way for strategies in precision medicine. Full article

Cataracts, characterized by the opacification of the eye lens, remain a leading cause of reversible blindness globally. Age and diabetes are key risk factors, and with the increasing aging and diabetic population, the global burden of cataracts is projected to rise significantly. Current treatment is predominantly surgical; however, pharmacological strategies could offer a non-invasive alternative with the potential to delay, prevent, or even reverse cataract progression. Recent research has enhanced our understanding of cataractogenesis, emphasizing oxidative stress as a key underlying mechanism, but also including other processes such as calcium dysregulation and altered lens homeostasis or specific events induced by hyperglycemia in diabetic cataracts. New therapeutic approaches have emerged considering the molecular mechanisms involved in cataracts, most of which focus on pharmacological agents with antioxidant properties. Additionally, small-molecule chaperones, aldose reductase inhibitors, and protein aggregation inhibitors have also demonstrated potential in stabilizing or restoring lens protein structure and transparency. While experimental results have shown encouraging results, further research is needed to optimize drug delivery systems to the lens, assess long-term safety, and confirm the clinical efficacy of these treatments. This article reviews current progress in pharmacological treatments for cataracts, outlining challenges and prospects for future integration into clinical practice. Full article

Draining wetland landscapes accelerates climate change, and multilateral support is therefore needed to speed up the transition to new land uses. This paper examines perceptions of ecosystem services (ES) in wetland areas in scientific and civic assessments. The case study area is Denmark’s largest drained wetland system, which is notable for its carbon sequestration potential. The area’s transformation efforts involving public participation offer a unique chance to examine differences between scientific and civic perceptions of ES. This exceptional case is ideal for revealing contextual differences, trade-offs, and controversies between scientific and civic perceptions of ES. Millennium ES Assessment and CICES are used as a conceptual framework for understanding and mapping human–nature interactions in a nature park. However, these systems are, in practice, not sufficiently developed to identify how citizens understand and value ES in real life. Therefore, we analyse perceptions using interviews, collaborative mapping, and media analysis. We compare these to scientific ES mappings based on local data, literature reviews, and fieldwork. The paper concludes that (1) scientific ES asymmetries are important; (2) environmental blind spots in scientific ES are due to its approach to knowledge collection; (3) citizens’ blind spots are due to their everyday life focus and tabooing the issue of local climate mitigation; and (4) science-based ES assessments and accounts are disconnected from local ES controversies. We argue that identifying ES controversies through various scientific methods may improve climate mitigation and restoration efforts if community planning becomes involved. Full article

Until now, the ultimate solution for blind people has not been achieved, because challenges still exist. Retinal implants have emerged as a promising solution for restoring vision in individuals suffering from retinal degenerative diseases such as retinitis pigmentosa and age-related macular degeneration. Central to the efficacy of these implants is the design and functionality of the electrode arrays responsible for stimulating retinal neurons. This review evaluates the evolution of retinal implants, with particular emphasis on electrode specifications, physiological considerations for electrical stimulation, and recent advancements in electrode design. A comprehensive analysis of state-of-the-art published studies provides a detailed cross-comparison of electrode characteristics, offering insights into current state-of-the-art technologies and future directions. Full article

With the rapid development of electronic technology, the electromagnetic interference encountered by airborne synthetic aperture radar (SAR) is no longer satisfied with a single type of interference, and it often encounters both suppressive and deceptive interference. In this manuscript, an algorithm based on blind signal separation (BSS) and deep residual learning is proposed for airborne SAR multi-electromagnetic interference suppression. Firstly, theoretical airborne SAR imaging in a multi-electromagnetic interference environment model is established, and the signal-mixed model of multi-electromagnetic interference is proposed. Then, a BSS algorithm using maximum kurtosis deconvolution and improved principal component analysis (PCA) is presented for suppressing the composite electromagnetic interference encountered by airborne SAR. Finally, in order to find the desired signal among multiple separated sources and to cope with the residual noise, a deep residual network is designed for signal recognition and denoising. This method uses a BSS algorithm with maximum kurtosis deconvolution and improved PCA to perform mixed signal separation. After performing signal separation, the original echo signal and the jamming can be obtained. To solve the separation order uncertainty and residual noise problems of the existing BSS algorithms, the deep residual network is designed to recognize airborne SAR signals after airborne SAR imaging. This algorithm has a better signal restoration degree, higher image restoration degree, and better compound interference suppression performance before and after anti-interference. Simulation and measurement results demonstrate the effectiveness of our presented algorithm. Full article

Acid-etched zirconia has emerged as a high-strength alternative to traditional glass ceramics for laminate veneers in aesthetic dentistry. This randomized, double-blind controlled clinical trial aimed to evaluate the one-year clinical performance of zirconia veneers etched with a hydrofluoric-nitric acid mixture and bonded using a 10-methacryloyloxydecyl dihydrogen phosphate (MDP) containing polymeric adhesive system, compared to lithium disilicate veneers. Fifty-two patients were treated with either translucent zirconia or lithium disilicate veneers, and restorations were bonded using light-cured resin-based adhesives. Clinical parameters, including veneer survival, esthetics, marginal adaptation, postoperative sensitivity, and periodontal health, were assessed using modified United States Public Health Service (USPHS) criteria and periodontal indexes at 2 weeks, 6 months, and 12 months. Both materials showed high survival rates with no statistically significant differences in clinical outcomes. One zirconia veneer debonded early but was successfully rebonded without fracture, while one lithium disilicate veneer fractured upon debonding. The findings support the viability of acid-etched zirconia veneers bonded with polymer-based adhesives as a durable and esthetic restorative option. The study highlights the clinical relevance of polymeric bonding systems in enhancing zirconia veneer performance and reinforces their role in modern adhesive dentistry. Full article

Background/Objectives: Primary insomnia is characterized by persistent sleeplessness that is not caused by medical, psychological, or environmental factors. It is defined by difficulty initiating or maintaining sleep for at least one month, leading to significant distress or impairment in daily functioning. This randomized, crossover, double-blind, placebo-controlled clinical trial aimed to assess the efficacy and tolerability of a Scutellaria lateriflora L. extract-based food supplement in subjects with mild to moderate primary insomnia. Methods: A total of 66 participants, aged 18–70 years, were randomly allocated into two groups (1:1 allocation ratio) and received either the food supplement (400 mg/day) or a placebo for 56 days, separated by a 28-day washout period. The clinical effectiveness of the food supplement was assessed using the Pittsburgh Sleep Quality Index (PSQI) as the primary outcome measure. Secondary outcomes included sleep-wake cycle parameters (sleep onset latency, sleep efficiency, and total sleep time) and a Visual Analog Scale (VAS). Results: A significant improvement in sleep-wake balance following supplementation with S. lateriflora, as reflected by enhanced scores in both primary and secondary outcomes, was observed. Furthermore, none of the participants reported adverse effects from the food supplement. Conclusions: Overall, these findings suggest that a S. lateriflora extract-based food supplement is a safe and effective strategy for restoring the sleep-wake cycle and improving quality of life in individuals with primary insomnia. Full article

Over the last decades, plenty of ancient manuscripts have been digitized all over the world, and particularly in Europe. The fruition of these huge digital archives is often limited by the bleed-through effect due to the acid nature of the inks used, resulting in very noisy images. Several authors have recently worked on bleed-through removal, using different approaches. With the aim of developing a bleed-through removal tool, capable of batch application on a large number of images, of the order of hundred thousands, we used machine learning and robust statistical methods with four different methods, and applied them to two medieval manuscripts. The methods used are (i) non-local means (NLM); (ii) Gaussian mixture models (GMMs); (iii) biweight estimation; and (iv) Gaussian blur. The application of these methods to the two quoted manuscripts shows that these methods are, in general, quite effective in bleed-through removal, but the selection of the method has to be performed according to the characteristics of the manuscript, e.g., if there is no ink fading and the difference between bleed-through pixels and the foreground text is clear, we can use a stronger model without the risk of losing important information. Conversely, if the distinction between bleed-through and foreground pixels is less pronounced, it is better to use a weaker model to preserve useful details. Full article

Background/Objectives: Adequate vitamin D levels are critical for overall health, yet vitamin D deficiency remains prevalent. This study aims to evaluate the efficacy and safety of a standardized weekly supplementation regimen of 100 μg calcifediol for patients with varying degrees of vitamin D deficiency. Methods: A post hoc pool analysis was conducted from a randomized, double-blind, placebo-controlled, multicenter, two-cohort trial. Cohort 1 included vitamin D mild deficiency patients (25(OH)D levels > 10 < 20 ng/mL) and Cohort 2 severe deficiency patients (25(OH)D levels ≤ 10 ng/mL). As both had placebo and weekly calcifediol 100 μg arms (ratio 1:2), a pooled analysis of safety and efficacy was conducted. The primary outcome was the percentage of subjects achieving 25(OH)D levels ≥ 20 ng/mL and/or ≥30 ng/mL at various time points. Results: A total of 401 participants across both cohorts were included in the analysis, 130 who received a placebo and 271 calcifediol 100 µg weekly. By week 52, 94.5% of individuals in the calcifediol group achieved 25(OH)D levels ≥ 20 ng/mL, compared to 25.3% in the placebo group (p < 0.0001). At this same week, 80.5% of subjects in the calcifediol group, but none in the placebo group (p < 0.0001), had 25(OH)D levels ≥ 30 ng/mL. The mean 25(OH)D level plateaued around 40.7 ng/mL from weeks 16 to 52. The frequency of treatment-emergent adverse events was similar in both groups, placebo and calcifediol. Conclusions: Weekly supplementation of 100 μg calcifediol effectively restores vitamin D levels in individuals with both mild and severe deficiencies, demonstrating a favourable safety profile. Full article

Background/Objectives: This double-blinded study aimed to evaluate the clinical performance of a giomer restorative material in comparison to a nanotechnology-based restorative system for the restoration of non-carious cervical lesions over a period of 48 months. Methods: A 48-month randomized, controlled, split-mouth trial was conducted with 49 subjects at its baseline, which was reduced to 34 subjects at follow-up, yielding a statistical power of 69.55%. Cervical lesions were restored using either BEAUTIFIL II LS (BL) or 3M/ESPE Filtek Supreme Universal Restorative (FS). Clinical assessments were performed by blinded examiners, excluding the one who placed the restorations. Evaluations were based on the Hickel criteria, covering esthetic, functional, and biological properties, with comparisons made using the Wilcoxon signed-rank test. Results: Hickel scores were analyzed with the Mann–Whitney U test to compare BL and FS groups within subjects. Changes in median Hickel scores, sensitivity, and the gingival index were assessed via Friedman’s test, followed by Wilcoxon signed-rank tests with a Bonferroni correction for post-hoc comparisons. The p-values under 0.05 were considered significant, except with the Bonferroni correction. Statistical analysis showed no significant differences between materials across most Hickel parameters (p > 0.05), though BL exhibited a trend of better surface staining (76.5% excellent with BL vs. 76.5% excellent with FS) and adjacent mucosa, while FS showed slight advantages in surface luster and color match. Conclusions: The clinical evaluation of restorations for non-carious cervical lesions using giomer and nanotechnology-based restorative systems revealed no statistically significant differences according to the Hickel criteria, indicating a similar clinical performance for both restorative materials. Full article

Several studies have suggested that adiponectin is an anti-aging molecule based on its potential involvement of adipose tissue in skin aging. In this study, we investigated the anti-photoaging efficacy of an adiponectin expression-stimulating peptide derivative, pentasodium tetracarboxymethyl hexanoyl dipeptide-12 (PTHD-12), in in vitro and ex vivo human skin explant models. A double-blind, randomized, comparator placebo-controlled study was performed to confirm clinical efficacy. After irradiation with 50 mJ/cm² of UVB, a UV-induced decrease in adiponectin expression and an increase in inflammatory cytokines in cultured human dermal fibroblasts were prevented by the PTHD-12 treatment test peptide. Mitigation of cellular senescence and senescence-associated secretory phenotype (SASP) expressions induced by UVB (50 mJ/cm²) exposure were also mitigated by the post-treatment of PTHD-12, which was also observed in an ex vivo human skin explant model. The restoration of filaggrin, loricrin, and claudin-1 protein expression in a cultured human skin explant was observed. A clinical study further confirmed that the restoration of UVB-induced skin damage, represented by increased skin redness and trans-epidermal water loss, was accelerated by the use of test peptide PTHD-12-containing products. These results suggest that targeting adiponectin may be a plausible strategy for the development of anti-aging ingredients. Full article

Background: In prosthodontics, achieving esthetic success requires precise shade matching of restorations to natural teeth. This study evaluated the shade-matching abilities of fourth-year dental students using different tools and conditions in laboratory (LB) and clinical (CL) settings. Methods: In the LB setting, students matched blinded shade tabs to the VITA classical shade guide under natural daylight (ND), artificial light (AL), and a polarized filter (PF). In the CL setting, they determined the shades of patients’ central incisors using the same conditions. Participants also completed a questionnaire about their experience. Results: Quantitative analysis showed better shade matching in the LB setting, particularly with the PF (52% correct), compared to ND (50%) and AL (43%). In the CL setting, overall accuracy dropped to 32% across all conditions. No results were statistically significant. Qualitative feedback revealed that students found the spectrophotometer to have improved their accuracy and reliability compared to the ND and AL conditions. Conclusions: This study highlights the importance of incorporating technology as a validation tool in dental practice. Leveraging tools like spectrophotometers may enhance shade-matching accuracy, streamline processes, and improve patient satisfaction while balancing traditional methods with technological advancements. Full article