Search Results (28)

Chemoresistance remains a major barrier to effective colorectal cancer (CRC) therapy, yet its metabolic underpinnings are poorly defined. Here, we integrate lipidomic profiling, enzymatic analysis, and functional perturbation approaches to elucidate the contribution of phosphatidylcholine (PC) metabolism and its biosynthetic regulator Choline Phosphotransferase 1 (CHPT1) to drug response. Comparative analysis of chemosensitive and chemoresistant CRC cell lines revealed that resistant HT29 cells exhibited significantly higher PC content and altered PC/lysophosphatidylcholine (LPC)ratios relative to sensitive counterparts. Importantly, functional perturbation confirmed causality: CHPT1 overexpression in SW620 cells was sufficient to promote PC accumulation and confer a chemoresistant phenotype. These findings identify CHPT1 as a metabolic gatekeeper of chemoresistance. Consistently, Human Protein Atlas survival analyses further support its clinical relevance, as elevated CHPT1 expression correlates with poor patient outcomes in CRC. Mechanistically, CHPT1-driven PC enrichment may sustain pro-survival signaling, while reducing lysophospholipid-mediated stress pathways. To therapeutically target this vulnerability, we investigated edelfosine (Edel), an alkyl-lysophospholipid that disrupts lipid rafts and inhibits PC biosynthesis upstream of CHPT1. Notably, edelfosine-mediated disruption of the Kennedy pathway enhances chemosensitivity in the resistant CRC model. Collectively, our study identifies CHPT1 and PC metabolism as central determinants of CRC drug response and proposes edelfosine-based metabolic reprogramming as a promising strategy to overcome resistance. Full article

Wine processing industries require a substantial amount of water and generate considerable volumes of wastewater. Winery wastewater (WWW) is notable for its high concentrations of biodegradable organic matter, while containing relatively low levels of nutrients. Due to seasonal variability in wastewater generation, treatment processes must be both efficient and adaptable. A range of wastewater treatment technologies are currently implemented at pilot and full scales, and ongoing research continues to yield innovative solutions in laboratory settings. This paper provides a comprehensive review of advancements in WWW treatment technologies, pinpoints gaps, and highlights future research directions. The treatment methods discussed include aerobic reactors, anaerobic systems, constructed wetlands (CWs) and biosand filters (BSFs), as well as advanced oxidation processes (AOPs). The advantages and limitations of these techniques, along with key factors affecting their performance, are examined. CWs are regarded as cost-effective and efficient solutions for small to medium wineries, whereas activated sludge and anaerobic digestion processes, which require a smaller footprint, are suitable for managing higher loads at large wineries. While anaerobic processes offer reduced operating costs, they often produce effluents of lower quality compared to aerobic processes, necessitating subsequent polishing prior to discharge. Advances in AOPs present promising alternatives for pre/post-treatment, facilitating the breakdown of persistent organics and achieving acceptable chemical oxygen demand (COD) levels. Nevertheless, further research is required to address operational optimization and reduce associated costs. Full article

The Centre for Affordable Water and Sanitation Technology (CAWST) (2012) recommends size standards for the effective size (ES) and uniformity coefficient (UC) of filtration media in biosand filters (BSFs) to ensure optimal effluent flow rates and contaminant removal. The recommended, laboratory-determined ES and UC ranges are also advised for field use despite differences in mass- versus volume-based protocols and no studies comparing laboratory versus field protocols to date. ES and UC values of five sand samples were compared using mass- versus volume-based measurements and lab versus field protocols. Results suggest that the use of mass- or volume-based measurements generally does not affect the ES or UC of a given method (except in cases where the density of sand grains varies significantly across the size distribution range). Differences between laboratory and field protocols, however, were found to affect ES and UC values by up to 24%. Overall, differences in laboratory and field sand size determination protocols should be further evaluated to ensure standardized field construction of BSFs and to limit potential impacts to filter efficacy and sustained, household filter usage. Full article

This paper applies a semi-quantitative approach to review the diversity, environmental controls, detection methods, human health risks, and mitigation of cyanotoxins in drinking water systems (DWSs). It discusses the environmental factors controlling the occurrence of cyanotoxins, presents the merits and limitations of emerging methods of their detection (qPCR, liquid chromatography–mass spectrometry, and electrochemical biosensors), and outlines the human exposure pathways and health outcomes with identification of high-risk groups and settings. High-risk groups include (1) communities relying on untreated drinking water from unsafe, polluted water sources and (2) low-income countries where cyanotoxins are not routinely monitored in DWSs. The fate and behavior processes are discussed, including removing cyanotoxins in DWSs based on conventional and advanced treatment processes. The available methods for cyanotoxin removal presented in this paper include (1) polymer-based adsorbents, (2) coagulation/flocculation, (3) advanced oxidation processes, (4) ultra- and nanofiltration, and (5) multi-soil layer systems. Future research should address (1) detection and fate in storage and conveyance facilities and at the point of consumption, (2) degradation pathways and toxicity of by-products or metabolites, (3) interactive health effects of cyanotoxins with legacy and emerging contaminants, (4) removal by low-cost treatment techniques (e.g., solar disinfection, boiling, bio-sand filtration, and chlorination), (5) quantitative health risk profiling of high-risk groups, and (6) epidemiological studies to link the prevalence of human health outcomes (e.g., cancer) to cyanotoxins in DWSs. Full article

To better understand diabetic nephropathy (DN), developing accurate animal models is crucial. Current models often fail to fully mimic human DN, showing only mild albuminuria, glomerular hypertrophy, and limited mesangial matrix expansion. Our study aims to develop a more robust model by combining streptozotocin (STZ)-induced diabetes with a high-protein diet (HPD). We divided C57Bl/6J mice into three groups: control, STZ with a standard diet (STZ-SD), and STZ with a HPD (45 kcal% protein) (STZ-HPD) for 12 weeks. Renal function was evaluated using the urinary albumin-to-creatinine ratio, and kidney tissues were analyzed for histological and molecular changes. The STZ-HPD group showed significantly higher albuminuria and more severe glomerular and tubular damage compared to the control and STZ-SD groups. These changes were accompanied by increased inflammatory and oxidative stress markers, highlighting the harmful effects of high-protein intake on renal injury. Our findings suggest that the STZ-HPD model could be a valuable tool for studying DN pathophysiology and evaluating therapeutic interventions, providing a new approach for preclinical research. Full article

Biosand reactors (BSRs), alternatively known as slow or biological sand filters, are passive systems that are used to remove contaminants from domestic wastewater, industrial wastewater and drinking water. This review focuses specifically on their application for remediation of industrial effluent and sewage-containing municipal and household effluent. The relationships between the physicochemical characteristics of the sand grains (size, size distribution, shape, chemical composition) and the hydraulic conductivity of the sand employed in BSFs are critically discussed in relationship to the achievable loading rates and hydraulic retention times. The modes of operation and influence of the functional microbial biomass as well as biodegradable and recalcitrant particulates on these parameters is comprehensively reviewed. Finally, the bioremediation of sewage-based and industrial wastewater is examined. This includes an account of the biotic and abiotic removal mechanisms and the limitations of BSRs for removal of pollutants such as phosphorus/phosphate and nitrates/nitrites. The removal mechanisms and removal efficiencies of macronutrients, micropollutants, fecal indicators and other microorganisms such as antibiotic-resistant bacteria in BSRs are discussed. Full article

Safe drinking water remains a major global challenge, especially in rural areas where, according to UNICEF, 80% of those without access to improved water systems reside. While water, sanitation, and hygiene (WASH)-related diseases and deaths are common outcomes of unsafe water, there is also an economic burden associated with unsafe water. These burdens are most prominent in rural areas in less-developed nations. Slow sand filters (SSFs), or biological sand filters (BSFs), are ideal water treatment solutions for these low-resource regions. SSFs are the oldest municipal drinking water treatment systems and improve water quality by removing suspended particles, dissolved organic chemicals, and other contaminants, effectively reducing turbidity and associated taste and odor problems. The removal of turbidity and dissolved organic compounds from the water enables the use of low-cost disinfection methods, such as chlorination. While the working principles of slow sand filtration have remained the same for over two centuries, the design, sizes, and application of slow sand filters have been customized over the years. This paper reviews these adaptations and recent reports on performance regarding contaminant removal. We specifically address the removal of turbidity and microbial contaminants, which are of great concern to rural populations in developing countries. Full article

Acidic wastewaters such as winery wastewater require treatment to increase the pH before discharge into the environment. Biosand filters have been shown to reduce the organic load while simultaneously providing a buffering function. Previous research has shown increases in pH which was assumed to mainly take place via dissolution of calcite from the sand particles. This study investigated the possible role of biotic mechanisms for pH adjustment in sand column experiments by comparing results obtained from irradiated (biotic) and non-irradiated (biotic and abiotic) sand columns extracted from biosand filters used to treat winery wastewater. The columns were fed with either synthetic winery wastewater or filtered water (control). It was shown that the specific hydroxide concentrations in the eluant from the non-irradiated columns was significantly (p < 0.05) higher than in the eluant from the irradiated columns (1.1 × 10⁻⁵ vs. 4.0 × 10⁻⁶ M/kgsand⁻¹), indicating the presence of both biotic (average 4.5 ± 0.13%) and abiotic (average 95.5 ± 0.16%) pH increases. Using multivariate statistical tools to analyze a combination of parameters linked with biotic and abiotic pH adjustment, significant differences (ANOVA, p < 0.05) were found between the four treatment groups (irradiated/non-irradiated SWW and control) and the groups showed good clustering in cluster plots (group average) linkages, and principal component analysis plots. Full article

Acidic effluent such as winery wastewater is challenging to remediate. Biological sand reactors can simultaneously remove organics and neutralize winery wastewater via biotic and abiotic mechanisms. The systems have been shown to be suitable for treating the intermittent flow of wastewater at small wineries. It has been shown that dissolution of calcite is the most important abiotic mechanism for increasing the pH of the influent. In this study, sand column experiments were used to determine the effects of (i) sand particle size distribution on calcite dissolution kinetics, and (ii) the effects of calcite particle dissolution on the hydraulic conductivity. The results were then used to calculate the theoretical temporal abiotic neutralization capacity of biological sand reactors with differently sized sand fractions, including unfractionated (raw) sand. The results were compared with those determined from a pilot system treating winery wastewater over a period of 3 years. Sand fractions with larger particles contained lower amounts of calcite (using Ca as a proxy), but exhibited higher hydraulic conductivities (3.0 ± 0.05 %Ca and 2.57 to 2.75 mm·s⁻¹, respectively) than those containing smaller particles and/or raw sand (4.8 ± 0.04 to 6.8 ± 0.03 %Ca and 0.19 to 1.25 mm·s⁻¹, respectively). The theoretical abiotic neutralization capacity of biological sand reactors was compared with a pilot system with the same flow rates, and a temporal abiotic neutralization capacity of 37 years was calculated for biological sand reactors, which compared favorably with the theoretical results obtained for wastewater with pH values between 2 (8.2 years) and 3 (82 years). It was concluded that biological sand filters with around 10% calcite will be able to abiotically neutralize winery wastewater and other wastewaters with similar acidities for the projected life span of the system. Future work should focus on determining the effect of sand grain size on the bioremediation capacity, as well as the use of biological sand reactors for treating other acidic organic wastewaters such as fruit processing, food production and distillery wastewater. Full article

In this study, granulated activated charcoal (GAC) and bio charcoal (BC) is used as a filler in P3 biosand bag filter to study their filtration performance against a range of fluoride impurities from 1–1400 mg/L. A set of experiments are done to analyze the filtration efficiency of the sandbag filter against fluoride impurities after incorporating different amounts (e.g., 0.2, 2 kg) and a combination of GAC and BC. A combination of filler GAC and BC (1 kg each) have exhibited excellent results with 100% fluoride removal efficiency against 5 mg/L fluoride impurities for an entire experimental time of 165 min. It is because of the synergetic effect of adsorption caused by the high surface area (739 m²/g) of GAC and hydroxyapatite groups in BC. The data from remediation experiments using individual GAC and BC are fitted into the Langmuir and Freundlich Isotherm Models to check their adsorption mechanism and determine GAC and BC’s maximum adsorption capacity (Q_m). The remediation data for both GAC and BC have shown the better fitting to the Langmuir Isotherm Model with a high R² value of 0.994 and 0.970, respectively, showing the excellent conformity with monolayer adsorption. While the GAC and BC have presented negative Kf values of −1.08 and −0.72, respectively, for Freundlich Model, showing the non-conformity to multilayer adsorption. The Q_m values obtained from Langmuir Model for GAC is 6.23 mg/g, and for BC, it is 9.13 mg/g. The pH study on adsorption efficiency of individual GAC and BC against 5 mg/L of fluoride impurities indicates the decrease in removal efficiency with an increase in pH from 3 to 9. For example, BC has shown removal efficiency of 99.8% at pH 3 and 99.5% at pH 9, while GAC has exhibited removal efficiency of 96.1% at pH 3 and 95.9% at pH 9. Importantly, this study presents the significance of the synergetic application of GAC and BC in the filters, where GAC and BC are different in their origin, functionalities, and surface characteristics. Full article

The lack of readily available sources of potable water is major problem in many parts of the world. This project engaged high school (HS) students in authentic and meaningful science and engineering activities to teach them about the lack and poor quality of potable water in many regions and how they can be addressed through the use of point of use (POU) treatments, such as biosand filters (BSFs). The HS students’ activities paralleled those of USF students, including research question development and BSF design, construction, operation, and monitoring. An ethnographic approach was utilized by incorporating participant observation, collection and review of artifacts, and interviews. It was found that the project’s focus on the need to provide potable water in the developing world provided authenticity and meaningfulness to the HS students, which encouraged their participation in activities and the learning of science and engineering practices. The HS students reported an awareness of the differences between this project and their regular science classes. The project had a positive impact on their perceptions of themselves as scientists and their interest in STEM careers. The HS students’ results were useful to the university-based research. In addition, the USF students gained teaching experience while investigating research questions in a low-stakes environment. Full article

This study assessed the impact of sanitation practices, hygienic and storage conditions on the quality of drinking water treated at point-of-use in Makwane Village. Subsequent to implementation of low-cost Household Water Treatment Devices which are the biosand filter with zeolite-silver (BSZ-SICG) and silver-impregnated porous pot (SIPP) filters in Makwane village, a structured questionnaire was designed to collect the following information: age of caretakers, number of children under the age of five, water storage conditions, sanitation amenities, and hygiene practices. Water quality from the sources to household level was assessed using culture-based and molecular techniques. The results revealed a significant association between the presence of Escherichia coli in treated drinking water with the age group of caregivers and the number of children ofless than the age of five [OR (95% CI) = 8.4737 (0.147–3.3497), p = 0.0141923 and OR (95% CI) = 9.1667 (0.1848–3.0159); p = 0.0165830, respectively]. Moreover, significant association was noted between hygiene practices (washing of hands with/without soap) and water quality in storage containers [OR (95% CI) = 16.000 (0.6763–3.9495), p = 0.0000125]. These findings further prove that there is still a dire need for reconsidering hygiene education in rural areas as the health benefits of water treated at point of use (POU) coupled with safe-storage condition interventions might not be guaranteed without proper hygiene. The results further highlighted the importance of washing hands in improving microbial quality of drinking water, which is the key factor for fighting against infectious diseases. Full article

Biological and chemical contamination of natural water bodies is a global health risk for more than one billion people, mostly living in low-income countries. Innovative, affordable, and efficient decentralized solutions for safe drinking water supply are urgently needed. Metallic iron (Fe⁰)-based filtration systems have been described as such an appropriate solution. This communication focuses on the Kanchan arsenic filter (KAF), presented in the early 2000s and widely assessed during the past decade. The KAF contains iron nails as the Fe⁰ source and is primarily designed to remove As from polluted tube well waters. Recent independent works assessing their performance have all reported on a high degree of variability in efficiency depending mostly on the following factors: (1) the current operating conditions, (2) the design, and (3) the groundwater chemistry. This communication shows that the major problems of the KAF are two-fold: (1) a design mistake as the Fe⁰ units disturb the operation and functionality of the biosand filter, and (2) the use of poorly characterized iron nails of unknown reactivity. This assertion is supported by the evidence that the very successful community filter designed by the Indian Institute of Technology Bombay works with iron nails and has been efficient for many years. Replacing iron nails by more reactive Fe⁰ materials (e.g., iron fillings and steel wool) should be tested in a new generation KAF. It is concluded that a methodological or systematic approach in introducing and monitoring the efficiency of KAF should be used to test and disseminate the next generation KAF worldwide. Moreover, better characterization of the Fe⁰ materials including their intrinsic reactivity is required. Full article

The current study investigated the acceptance rate and long-term effectiveness of cost-effective household water treatment systems deployed in Makwane Village. A structured questionnaire was used prior to implementation to collect information such as level of education, level of employment, and knowledge about point-of-use water treatment systems in the target area. The long-term effectiveness was determined by factors such as the Escherichia coli removal efficiency, turbidity reduction, silver leached, and flow rate of the household water treatment devices. The results of the survey prior to deployment revealed that only 4.3% of the community had a tertiary qualification. Moreover, 54.3% of the community were unemployed. The results further revealed that 65.9% of the community were knowledgeable about other point-of-use water treatment methods. The acceptance rate, which was found to be initially higher (100%), reduced after three months of implantation (biosand filter with zeolite-silver clay granular—82.9%; silver-impregnated porous pot filters—97.1%). Moreover, the long-term effectiveness was determined, taking into consideration the adoption rate, and it was found that silver-impregnated porous pot filters have a long life compared to biosand filter with zeolite-silver clay granular. Although household water treatment systems can effectively reduce the burden of waterborne diseases in impoverished communities, the success of adoption is dependent on the targeted group. This study highlights the significance of involving community members when making the decision to scale up household water treatment devices in rural areas for successful adoption. Full article